Rroxscaffold_1G00020290

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001754
Physical Location & Seq
Forward (+)
24637441 .. 24640098
2658 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00020290.1

Sequence Viewer

Length: 1560 bp
ATGGGAGACGAGGATTGGTTCTTGTTGCAGAACTCAAAGCAGGTGGTGAAAACTCGAGCTGGTGAAATGAGGGTGGTGAAAAGCGTAGGTGGGAGGATTGTTGATAAGCCAATGCATATTGGGTTTATTACAATGGAGCCCAAGTCCCTCTTTGTACCTCAGTACATGGACTCCAGCTTGATCCTCTTCGTCCATTCTGGGGAAGCAAAGGTAGGGCTGATATACAGAGATGAACTGGGAGAGAGGAGATTGAGGGCTGGGGATGTGTACAGAATTCCAGCTGGTTCGCCGTTTTATTTGGTGAATACAGGGGAGGGTCAGAGACTTCACATCATCTGTAGCGTTGATACATCTGAGAGTTTGGGAATGGGTACTTTGCAGTCTTTCTACATTGGTGGAGGATATAACCCAGCATCTGTACTTGCTGGATTTGACCAGGAAATACTCGAAAGTGCATTCAATGTTACATCATCAGAACTGAGTGAATTCTTGTCAAGGCAACAAGATGGTCCGATTGTATATGTGACTGATTCACCTCATCCACCAAGCCTTTGGTCAAGATTCCTAGAGCTGAAAGAGCATGACAGACTCCAAAATATGAAGAGAATGGTGGGCTTCACAAAAGAACCTGATCACCAAGAAGAAGACCATTCATTAGCATGGTCATGGAGGAAGCTTCTCAACTCTGTGTTCGGATCAAATGACAAGAGAAGAGACGATCCCGATGCTTACAAGGGAAAAGGAAAATCACATGATGCTTACAACCTCTTCAAGAGATCTGCAGATTTCAAAAACGACTATGGCTGGAGCAAGGCACTTGACGAATCTGATTACTCGCCACTAAAAGACTCTGGCGTTGGTGTCTTCCTTGTCAACCTTTCTGCAGGGTCAATGATGGCACCACACGTGAACCCAATTGCAACAGAATATGGGATTGTATTGAGTGGCTCTGGCACAATACAGATTGTATTTCCAAACGGAACCTCGGCAATGAATACTAAAGTTCAAGAAGGAGATGTGTTTTGGGTCCCAAGGTACTTCCCATTCTGTCAAATTGCATCAAGAACTGGTCCTCTTGAGTTCTTTGGATTCACCACCTCGGCACGCAAGAACAGGCCACAGTTCTTGGTTGGTGCTAGCTCTGTTCTTAGGGAGCTCAGAGGACCTGAGCTTGCAGCTGCTTTTGGTGTGAATGAGGATCGGTTGAGTCGCATTGTTCATGCTCAGCGTGAGTCTGTGATCTTGCCTTCGGTACAAGCAGCTCCTCCTTACAAGGGGGAGGAGAGAGAGCCAACCCCGGAGGAGGAGACAGGGAAGCAAACCCCATACAAGGAGGACGAGAGGAGGCAGGAAGACAGGAGGAGGCGGGAAGACGAGAGGGAGCAATCAGCTGAGGAAGATGAGAGAAGGCAACCCCCATACAAGGAAGATGAGAGGCAGCAGTCGTCACAGGAGAAGCAGAGGAGGCAACCTCCATACGGGGAAGGGGATGTGATGTTTCAGAGGCTCCCAAGGGTGATCAAGAGCTTCGGGAATGAGATGGTTATGGGTTTTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

519

Amino Acids

58.82

Weight (kDa)

5.67

Isoelectric Point (pI)

54.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 41 - 159 3.4e-09 Cupin
Cupin_1 PF00190 254 - 399 1.2e-32 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 31
Acc36I ACCTGC 1 cut(s) 31
AccB1I GGYRCC 1 cut(s) 898
AciI CCGC 1 cut(s) 1366
AclWI GGATC 4 cut(s) 175, 703, 713, 1206
AcsI RAATTY 2 cut(s) 273, 485
AcvI CACGTG 1 cut(s) 907
AfaI GTAC 7 cut(s) 156, 164, 269, 373, 420, 1037, 1254
AfiI CCNNNNNNNGG 6 cut(s) 199, 1274, 1303, 1330, 1423, 1478
AflIII ACRYGT 1 cut(s) 904
AgsI TTSAA 4 cut(s) 460, 772, 790, 1007
AjnI CCWGG 1 cut(s) 435
Alw21I GWGCWC 1 cut(s) 1158
Alw26I GTCTC 3 cut(s) 316, 708, 1301
AlwI GGATC 4 cut(s) 175, 703, 713, 1206
AlwNI CAGNNNCTG 1 cut(s) 416
Ama87I CYCGRG 1 cut(s) 54
AoxI GGCC 1 cut(s) 1115
ApeKI GCWGC 4 cut(s) 1175, 1178, 1259, 1438
ApoI RAATTY 2 cut(s) 273, 485
AspS9I GGNCC 4 cut(s) 509, 1027, 1070, 1163
AsuC2I CCSGG 1 cut(s) 1298
AsuHPI GGTGA 8 cut(s) 58, 74, 88, 313, 525, 626, 1084, 1528
AsuNHI GCTAGC 1 cut(s) 1136
AvaI CYCGRG 1 cut(s) 54
AvaII GGWCC 4 cut(s) 509, 1027, 1070, 1163
BanI GGYRCC 1 cut(s) 898
BanII GRGCYC 2 cut(s) 141, 1158
BbrPI CACGTG 1 cut(s) 907
BbsI GAAGAC 4 cut(s) 651, 856, 1359, 1377
Bbv12I GWGCWC 1 cut(s) 1158
BbvCI CCTCAGC 1 cut(s) 1392
BbvI GCAGC 4 cut(s) 1165, 1187, 1271, 1450
BccI CCATC 3 cut(s) 500, 889, 1534
BceAI ACGGC 1 cut(s) 274
BcgI CGANNNNNNTGC 2 cut(s) 707, 741
BciT130I CCWGG 1 cut(s) 437
BclI TGATCA 2 cut(s) 631, 1518
BcnI CCSGG 1 cut(s) 1298
BcoDI GTCTC 3 cut(s) 316, 708, 1301
BfaI CTAG 2 cut(s) 566, 1137
BfmI CTRYAG 3 cut(s) 337, 780, 882
BfuAI ACCTGC 1 cut(s) 31
BglII AGATCT 1 cut(s) 776
BisI GCNGC 4 cut(s) 1176, 1179, 1260, 1439
BlpI GCTNAGC 1 cut(s) 1224
BlsI GCNGC 4 cut(s) 1177, 1180, 1261, 1440
Bme1390I CCNGG 2 cut(s) 437, 1298
Bme18I GGWCC 4 cut(s) 509, 1027, 1070, 1163
BmeT110I CYCGRG 1 cut(s) 54
BmgT120I GGNCC 4 cut(s) 509, 1027, 1070, 1163
BmiI GGNNCC 6 cut(s) 138, 900, 982, 1028, 1029, 1508
BmrFI CCNGG 2 cut(s) 437, 1298
BmrI ACTGGG 1 cut(s) 245
BmsI GCATC 4 cut(s) 422, 715, 745, 1067
BmtI GCTAGC 1 cut(s) 1140
BmuI ACTGGG 1 cut(s) 245
BpiI GAAGAC 4 cut(s) 651, 856, 1359, 1377
BplI GAGNNNNNCTC 2 cut(s) 1456, 1488
BpmI CTGGAG 2 cut(s) 157, 826
Bpu10I CCTNAGC 2 cut(s) 1167, 1392
Bpu1102I GCTNAGC 1 cut(s) 1224
BpuEI CTTGAG 1 cut(s) 1097
BpuMI CCSGG 1 cut(s) 1298
BsaAI YACGTR 1 cut(s) 907
BsaJI CCNNGG 5 cut(s) 984, 1031, 1098, 1296, 1511
BsaXI ACNNNNNCTCC 4 cut(s) 128, 155, 158, 185
Bsc4I CCNNNNNNNGG 6 cut(s) 199, 1274, 1303, 1330, 1423, 1478
Bse1I ACTGG 2 cut(s) 240, 1072
Bse3DI GCAATG 1 cut(s) 996
BseBI CCWGG 1 cut(s) 437
BseDI CCNNGG 5 cut(s) 984, 1031, 1098, 1296, 1511
BseGI GGATG 3 cut(s) 268, 538, 1495
BseLI CCNNNNNNNGG 6 cut(s) 199, 1274, 1303, 1330, 1423, 1478
BseMI GCAATG 1 cut(s) 996
BseMII CTCAG 7 cut(s) 173, 345, 470, 1158, 1171, 1238, 1383
BseNI ACTGG 2 cut(s) 240, 1072
BseRI GAGGAG 8 cut(s) 259, 1254, 1295, 1316, 1319, 1357, 1375, 1477
BseXI GCAGC 4 cut(s) 1165, 1187, 1271, 1450
BseYI CCCAGC 2 cut(s) 257, 409
BshFI GGCC 1 cut(s) 1117
BshNI GGYRCC 1 cut(s) 898
BsiHKAI GWGCWC 1 cut(s) 1158
BsiHKCI CYCGRG 1 cut(s) 54
BsiSI CCGG 1 cut(s) 1298
BslFI GGGAC 2 cut(s) 130, 1013
BslI CCNNNNNNNGG 6 cut(s) 199, 1274, 1303, 1330, 1423, 1478
BsmAI GTCTC 3 cut(s) 316, 708, 1301
BsmBI CGTCTC 1 cut(s) 708
BsmFI GGGAC 2 cut(s) 130, 1013
BsmI GAATGC 1 cut(s) 455
BsnI GGCC 1 cut(s) 1117
BsoBI CYCGRG 1 cut(s) 54
Bsp1286I GDGCHC 2 cut(s) 141, 1158
Bsp1407I TGTACA 1 cut(s) 267
Bsp143I GATC 8 cut(s) 180, 631, 695, 718, 776, 1198, 1239, 1518
Bsp1720I GCTNAGC 1 cut(s) 1224
BspACI CCGC 1 cut(s) 1366
BspANI GGCC 1 cut(s) 1117
BspCNI CTCAG 7 cut(s) 172, 346, 471, 1159, 1170, 1237, 1384
BspLI GGNNCC 6 cut(s) 138, 900, 982, 1028, 1029, 1508
BspMAI CTGCAG 2 cut(s) 784, 886
BspMI ACCTGC 1 cut(s) 31
BspOI GCTAGC 1 cut(s) 1140
BspPI GGATC 4 cut(s) 175, 703, 713, 1206
BspT107I GGYRCC 1 cut(s) 898
BsrDI GCAATG 1 cut(s) 996
BsrGI TGTACA 1 cut(s) 267
BsrI ACTGG 2 cut(s) 240, 1072
BssECI CCNNGG 5 cut(s) 984, 1031, 1098, 1296, 1511
BssMI GATC 8 cut(s) 180, 631, 695, 718, 776, 1198, 1239, 1518
BssT1I CCWWGG 2 cut(s) 1031, 1511
Bst2UI CCWGG 1 cut(s) 437
Bst4CI ACNGT 1 cut(s) 1122
Bst6I CTCTTC 4 cut(s) 191, 596, 706, 773
BstAUI TGTACA 1 cut(s) 267
BstBAI YACGTR 1 cut(s) 907
BstC8I GCNNGC 3 cut(s) 1105, 1138, 1173
BstDEI CTNAG 8 cut(s) 159, 354, 479, 1148, 1157, 1167, 1224, 1392
BstF5I GGATG 3 cut(s) 268, 538, 1495
BstKTI GATC 8 cut(s) 183, 634, 698, 721, 779, 1201, 1242, 1521
BstMAI GTCTC 3 cut(s) 316, 708, 1301
BstMBI GATC 8 cut(s) 180, 631, 695, 718, 776, 1198, 1239, 1518
BstMWI GCNNNNNNNGC 2 cut(s) 577, 1465
BstNI CCWGG 1 cut(s) 437
BstSCI CCNGG 2 cut(s) 435, 1296
BstSFI CTRYAG 3 cut(s) 337, 780, 882
BstV1I GCAGC 4 cut(s) 1165, 1187, 1271, 1450
BstV2I GAAGAC 4 cut(s) 651, 856, 1359, 1377
BstX2I RGATCY 1 cut(s) 776
BstXI CCANNNNNNTGG 1 cut(s) 552
BstYI RGATCY 1 cut(s) 776
BsuRI GGCC 1 cut(s) 1117
BtsCI GGATG 3 cut(s) 268, 538, 1495
BveI ACCTGC 1 cut(s) 31
Cac8I GCNNGC 3 cut(s) 1105, 1138, 1173
CaiI CAGNNNCTG 1 cut(s) 416
Cfr13I GGNCC 4 cut(s) 509, 1027, 1070, 1163
Csp6I GTAC 7 cut(s) 155, 163, 268, 372, 419, 1036, 1253
CspCI CAANNNNNGTGG 4 cut(s) 24, 59, 1107, 1142
CviAII CATG 6 cut(s) 166, 581, 660, 666, 752, 1220
CviQI GTAC 7 cut(s) 155, 163, 268, 372, 419, 1036, 1253
DdeI CTNAG 8 cut(s) 159, 354, 479, 1148, 1157, 1167, 1224, 1392
DpnI GATC 8 cut(s) 182, 633, 697, 720, 778, 1200, 1241, 1520
DpnII GATC 8 cut(s) 180, 631, 695, 718, 776, 1198, 1239, 1518
Eam1104I CTCTTC 4 cut(s) 191, 596, 706, 773
EarI CTCTTC 4 cut(s) 191, 596, 706, 773
Ecl136II GAGCTC 1 cut(s) 1156
Eco130I CCWWGG 2 cut(s) 1031, 1511
Eco24I GRGCYC 2 cut(s) 141, 1158
Eco47I GGWCC 4 cut(s) 509, 1027, 1070, 1163
Eco53kI GAGCTC 1 cut(s) 1156
Eco72I CACGTG 1 cut(s) 907
Eco88I CYCGRG 1 cut(s) 54
EcoICRI GAGCTC 1 cut(s) 1156
EcoO109I RGGNCCY 2 cut(s) 1027, 1163
EcoRI GAATTC 2 cut(s) 273, 485
EcoRII CCWGG 1 cut(s) 435
EcoT14I CCWWGG 2 cut(s) 1031, 1511
EcoT22I ATGCAT 1 cut(s) 117
EcoT38I GRGCYC 2 cut(s) 141, 1158
ErhI CCWWGG 2 cut(s) 1031, 1511
Esp3I CGTCTC 1 cut(s) 708
FaeI CATG 6 cut(s) 169, 584, 663, 669, 755, 1223
FalI AAGNNNNNCTT 2 cut(s) 134, 166
FaqI GGGAC 2 cut(s) 130, 1013
FatI CATG 6 cut(s) 165, 580, 659, 665, 751, 1219
FauI CCCGC 1 cut(s) 1359
FbaI TGATCA 2 cut(s) 631, 1518
Fnu4HI GCNGC 4 cut(s) 1176, 1179, 1260, 1439
FokI GGATG 3 cut(s) 275, 525, 1502
FriOI GRGCYC 2 cut(s) 141, 1158
Fsp4HI GCNGC 4 cut(s) 1176, 1179, 1260, 1439
FspBI CTAG 2 cut(s) 566, 1137
GluI GCNGC 4 cut(s) 1176, 1179, 1260, 1439
GsaI CCCAGC 2 cut(s) 261, 413
GsuI CTGGAG 2 cut(s) 157, 826
HaeIII GGCC 1 cut(s) 1117
HapII CCGG 1 cut(s) 1298
Hin1II CATG 6 cut(s) 169, 584, 663, 669, 755, 1223
HincII GTYRAC 1 cut(s) 874
HindII GTYRAC 1 cut(s) 874
HindIII AAGCTT 1 cut(s) 674
HinfI GANTC 9 cut(s) 170, 530, 561, 588, 824, 848, 1089, 1207, 1232
HpaII CCGG 1 cut(s) 1298
HphI GGTGA 8 cut(s) 58, 74, 88, 313, 525, 626, 1084, 1528
Hpy166II GTNNAC 3 cut(s) 268, 874, 910
Hpy188I TCNGA 8 cut(s) 321, 355, 475, 513, 695, 829, 1160, 1503
Hpy188III TCNNGA 8 cut(s) 558, 722, 772, 1007, 1062, 1076, 1522, 1531
Hpy8I GTNNAC 3 cut(s) 268, 874, 910
HpyAV CCTTC 4 cut(s) 1004, 1257, 1401, 1478
HpyCH4III ACNGT 1 cut(s) 1122
HpyCH4IV ACGT 1 cut(s) 906
HpyCH4V TGCA 9 cut(s) 28, 115, 379, 455, 782, 884, 920, 1058, 1175
HpyF10VI GCNNNNNNNGC 2 cut(s) 577, 1465
HpyF3I CTNAG 8 cut(s) 159, 354, 479, 1148, 1157, 1167, 1224, 1392
HpySE526I ACGT 1 cut(s) 906
Hsp92II CATG 6 cut(s) 169, 584, 663, 669, 755, 1223
KflI GGGWCCC 1 cut(s) 1027
Ksp22I TGATCA 2 cut(s) 631, 1518
Kzo9I GATC 8 cut(s) 180, 631, 695, 718, 776, 1198, 1239, 1518
LmnI GCTCC 6 cut(s) 136, 807, 1153, 1267, 1381, 1512
Lsp1109I GCAGC 4 cut(s) 1165, 1187, 1271, 1450
LweI GCATC 4 cut(s) 422, 715, 745, 1067
MaeI CTAG 2 cut(s) 566, 1137
MaeII ACGT 1 cut(s) 906
MaeIII GTNAC 3 cut(s) 463, 523, 1446
MalI GATC 8 cut(s) 182, 633, 697, 720, 778, 1200, 1241, 1520
MboI GATC 8 cut(s) 180, 631, 695, 718, 776, 1198, 1239, 1518
MfeI CAATTG 1 cut(s) 915
MflI RGATCY 1 cut(s) 776
MhlI GDGCHC 2 cut(s) 141, 1158
MluCI AATT 4 cut(s) 273, 485, 915, 1053
MlyI GAGTC 5 cut(s) 164, 582, 842, 1216, 1241
Mph1103I ATGCAT 1 cut(s) 117
MslI CAYNNNNRTG 2 cut(s) 658, 664
MspA1I CMGCKG 3 cut(s) 281, 1178, 1391
MspI CCGG 1 cut(s) 1298
MspR9I CCNGG 2 cut(s) 437, 1298
MunI CAATTG 1 cut(s) 915
Mva1269I GAATGC 1 cut(s) 455
MvaI CCWGG 1 cut(s) 437
MwoI GCNNNNNNNGC 2 cut(s) 577, 1465
NciI CCSGG 1 cut(s) 1298
NdeII GATC 8 cut(s) 180, 631, 695, 718, 776, 1198, 1239, 1518
NheI GCTAGC 1 cut(s) 1136
NlaIII CATG 6 cut(s) 169, 584, 663, 669, 755, 1223
NlaIV GGNNCC 6 cut(s) 138, 900, 982, 1028, 1029, 1508
NmeAIII GCCGAG 2 cut(s) 965, 1079
NmuCI GTSAC 2 cut(s) 523, 1446
NsiI ATGCAT 1 cut(s) 117
PaeR7I CTCGAG 1 cut(s) 54
PaqCI CACCTGC 1 cut(s) 31
PctI GAATGC 1 cut(s) 455
PfeI GAWTC 4 cut(s) 530, 561, 824, 1089
PkrI GCNGC 4 cut(s) 1177, 1180, 1261, 1440
PleI GAGTC 5 cut(s) 164, 582, 842, 1215, 1240
PmaCI CACGTG 1 cut(s) 907
PmlI CACGTG 1 cut(s) 907
PpsI GAGTC 5 cut(s) 164, 582, 842, 1215, 1240
Ppu21I YACGTR 1 cut(s) 907
PpuMI RGGWCCY 2 cut(s) 1027, 1163
Psp124BI GAGCTC 1 cut(s) 1158
Psp5II RGGWCCY 2 cut(s) 1027, 1163
Psp6I CCWGG 1 cut(s) 435
PspCI CACGTG 1 cut(s) 907
PspFI CCCAGC 2 cut(s) 257, 409
PspGI CCWGG 1 cut(s) 435
PspN4I GGNNCC 6 cut(s) 138, 900, 982, 1028, 1029, 1508
PspPI GGNCC 4 cut(s) 509, 1027, 1070, 1163
PspPPI RGGWCCY 2 cut(s) 1027, 1163
PspXI VCTCGAGB 1 cut(s) 54
PstI CTGCAG 2 cut(s) 784, 886
PstNI CAGNNNCTG 1 cut(s) 416
PsuI RGATCY 1 cut(s) 776
PvuII CAGCTG 3 cut(s) 281, 1178, 1391
RsaI GTAC 7 cut(s) 156, 164, 269, 373, 420, 1037, 1254
RsaNI GTAC 7 cut(s) 155, 163, 268, 372, 419, 1036, 1253
RseI CAYNNNNRTG 2 cut(s) 658, 664
SacI GAGCTC 1 cut(s) 1158
SatI GCNGC 4 cut(s) 1176, 1179, 1260, 1439
Sau3AI GATC 8 cut(s) 180, 631, 695, 718, 776, 1198, 1239, 1518
Sau96I GGNCC 4 cut(s) 509, 1027, 1070, 1163
SchI GAGTC 5 cut(s) 164, 582, 842, 1216, 1241
ScrFI CCNGG 2 cut(s) 437, 1298
SduI GDGCHC 2 cut(s) 141, 1158
SfaNI GCATC 4 cut(s) 422, 715, 745, 1067
SfcI CTRYAG 3 cut(s) 337, 780, 882
Sfr274I CTCGAG 1 cut(s) 54
SinI GGWCC 4 cut(s) 509, 1027, 1070, 1163
SlaI CTCGAG 1 cut(s) 54
SmiMI CAYNNNNRTG 2 cut(s) 658, 664
SmlI CTYRAG 2 cut(s) 54, 1076
SmoI CTYRAG 2 cut(s) 54, 1076
Sse9I AATT 4 cut(s) 273, 485, 915, 1053
SsiI CCGC 1 cut(s) 1366
SspMI CTAG 2 cut(s) 566, 1137
SstI GAGCTC 1 cut(s) 1158
StyD4I CCNGG 2 cut(s) 435, 1296
StyI CCWWGG 2 cut(s) 1031, 1511
TaaI ACNGT 1 cut(s) 1122
TaiI ACGT 1 cut(s) 909
TaqI TCGA 2 cut(s) 55, 447
TasI AATT 4 cut(s) 273, 485, 915, 1053
TatI WGTACW 3 cut(s) 162, 267, 418
TfiI GAWTC 4 cut(s) 530, 561, 824, 1089
TseFI GTSAC 2 cut(s) 523, 1446
TseI GCWGC 4 cut(s) 1175, 1178, 1259, 1438
Tsp45I GTSAC 2 cut(s) 523, 1446
TspDTI ATGAA 5 cut(s) 246, 614, 642, 1007, 1208
TspGWI ACGGA 1 cut(s) 993
VpaK11BI GGWCC 4 cut(s) 509, 1027, 1070, 1163
XapI RAATTY 2 cut(s) 273, 485
XcmI CCANNNNNNNNNTGG 1 cut(s) 549
XhoI CTCGAG 1 cut(s) 54
XspI CTAG 2 cut(s) 566, 1137
Zsp2I ATGCAT 1 cut(s) 117
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.