Rh5BG407700

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0001754
Physical Location & Seq
Reverse (-)
65970310 .. 66162932
192623 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG407700.1

Sequence Viewer

Length: 1416 bp
ATGGGAAACAGAGTGATGGTGAGCCTCCTGGTTCTTGTGCTTGTGCTTTCCTATGGAGTTATGGCAATGGCTATGAGGTTTAATCATGAAGATGAAGAGTGGGGAAGAGATCAGAGAGAAGAAGAGTGGGAAGGGCACCGAGGGCATCGCCATCTTTTCCTTTTGCAACAATCAAGGCTCCTGGTGAAAACTGAAGCTGGGCAAATGAGGGTGGTGAGAAGTGTAGGTCAGAGAATGGTAGATAGGCATATAAATGTTGCCTATCTCACCTTGGAACCTAACTCCCTCTTCATCCCCCAGTACCTCGACTCCAGCTTGATCCTCTTTGTCCAAAGAGGGCAAGCAAATGTGGGACTGGTACACCGACAGGAATTGGGGGAGAGACAATTGAAGGCTGGGGATGTGTACAGAATTCCAGCAGGTTCCACATTTTACTTGCAGAATGTCAGGGAGAACAGGAGACTTCAGATCATTCTCAGCATCGACACATCTGACAGTTTGAGAATGGGTACTTTGGAGAATTTCTTCATTGGTGGAGGAACCTACCCAAAATCTGTACTTTCTGGGTTCGATTCTGAGATACTCACAAATGCATTTAACGTCTCATCTTCAGAACTAAAACACATTTTGACAAGGCAACAAGAGGGTCCAATTGTATCTCTGAATAATTCTCAATCATCATCAAACCTTTGGACAAAATTCCTAAGCATGAAAGAGCAGGACAGACTACAAGAACTAAAGAAAATGGTCGACTTCCCACAAGAACAAGAGCAAACCCAAACATGGTCATGGAGGAAACTCTTGACCTCTATGTTCGGAACAATCGAAAACGAGAAGAGGAAGCAAAATCATCATGAGCACTCCCAGAAGGCTTACAACCTCTATGCGAGGAGGCCGGATTTCAAGAACAACTATGGATCGAGCTTGTCAGTTGATGAGTCTGATTACTCACCGCTACAAAACTCCGGCATTGGCGTTTACCTTGTCAATCTTAACGCAGGATCGATGTTGGCACCACATGTGAACCCAGCTGCAACAGAATACGGCGTCGTTCTAAGAGGCTCAGGATCACTCCAGATTGTGTTTCCAAACGGAACCTTGGCAATGAAGGCGAAACTAAAACCAGGGAGAGTGTTCTGGGTTCCGAGGTACTTCCCATTTGTCCAGGTGGCATCTAACAACGAGCAACTTCAGATTTTCGGGTTCACTACCTCAAAGCAGCCGAACCACCCACAATTTCTGGCTGGTTCTAGCTCGGTGCTTGAAGCTCTAAGAGGTCCGGAACTTGCAGCTGCTTTTGGTGTGAGTGAGGGAAGGTTGAATCGTTTTGTTAATGCTCAGCGCGAAGCTGTAATCTTACCTTACAATGCGGTGAGGCATCATGAGACACATGGGATCCCCATGGCGGACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

471

Amino Acids

53.46

Weight (kDa)

9.17

Isoelectric Point (pI)

45.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 55 - 205 5.4e-12 Cupin
Cupin_1 PF00190 292 - 437 1e-30 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 410
AccB1I GGYRCC 2 cut(s) 135, 1012
AccI GTMKAC 1 cut(s) 750
AccII CGCG 1 cut(s) 1344
AccIII TCCGGA 1 cut(s) 1279
AciI CCGC 3 cut(s) 953, 1370, 1406
AclWI GGATC 6 cut(s) 313, 925, 1009, 1075, 1390, 1403
AcsI RAATTY 3 cut(s) 411, 520, 698
AcuI CTGAAG 4 cut(s) 213, 449, 594, 1175
AcyI GRCGYC 1 cut(s) 1047
AfaI GTAC 6 cut(s) 302, 360, 407, 511, 558, 1151
AfiI CCNNNNNNNGG 2 cut(s) 783, 1405
AflIII ACRYGT 1 cut(s) 1018
AgsI TTSAA 4 cut(s) 391, 904, 1265, 1321
AjnI CCWGG 4 cut(s) 27, 180, 1123, 1164
AluBI AGCT 8 cut(s) 197, 315, 924, 1031, 1254, 1268, 1292, 1349
AluI AGCT 8 cut(s) 197, 315, 924, 1031, 1254, 1268, 1292, 1349
Alw21I GWGCWC 1 cut(s) 861
Alw26I GTCTC 4 cut(s) 376, 454, 607, 1379
AlwI GGATC 6 cut(s) 313, 925, 1009, 1075, 1390, 1403
Aor13HI TCCGGA 1 cut(s) 1279
AoxI GGCC 1 cut(s) 893
ApeKI GCWGC 4 cut(s) 1031, 1219, 1289, 1292
ApoI RAATTY 3 cut(s) 411, 520, 698
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 1 cut(s) 1344
AspS9I GGNCC 2 cut(s) 647, 1277
AsuHPI GGTGA 6 cut(s) 31, 196, 226, 259, 942, 1384
AvaII GGWCC 2 cut(s) 647, 1277
BaeGI GKGCMC 1 cut(s) 138
BamHI GGATCC 1 cut(s) 1395
BanI GGYRCC 2 cut(s) 135, 1012
Bbv12I GWGCWC 1 cut(s) 861
BbvI GCAGC 4 cut(s) 1018, 1231, 1279, 1301
BccI CCATC 2 cut(s) 10, 159
BceAI ACGGC 1 cut(s) 1060
BciT130I CCWGG 4 cut(s) 29, 182, 1125, 1166
BcoDI GTCTC 4 cut(s) 376, 454, 607, 1379
BfaI CTAG 1 cut(s) 1251
BfuAI ACCTGC 1 cut(s) 410
BisI GCNGC 4 cut(s) 1032, 1220, 1290, 1293
BlpI GCTNAGC 1 cut(s) 1338
BlsI GCNGC 4 cut(s) 1033, 1221, 1291, 1294
Bme1390I CCNGG 4 cut(s) 29, 182, 1125, 1166
Bme18I GGWCC 2 cut(s) 647, 1277
BmgT120I GGNCC 2 cut(s) 647, 1277
BmrFI CCNGG 4 cut(s) 29, 182, 1125, 1166
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 4 cut(s) 154, 489, 1181, 1387
BmuI ACTGGG 1 cut(s) 292
BpmI CTGGAG 2 cut(s) 295, 1058
Bpu10I CCTNAGC 2 cut(s) 704, 1063
Bpu1102I GCTNAGC 1 cut(s) 1338
Bsa29I ATCGAT 1 cut(s) 1004
BsaHI GRCGYC 1 cut(s) 1047
BsaJI CCNNGG 6 cut(s) 139, 270, 1098, 1124, 1145, 1401
BsaWI WCCGGW 1 cut(s) 1279
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 2 cut(s) 783, 1405
Bse1I ACTGG 3 cut(s) 298, 360, 1415
Bse3DI GCAATG 2 cut(s) 72, 1110
BseAI TCCGGA 1 cut(s) 1279
BseBI CCWGG 4 cut(s) 29, 182, 1125, 1166
BseCI ATCGAT 1 cut(s) 1004
BseDI CCNNGG 6 cut(s) 139, 270, 1098, 1124, 1145, 1401
BseGI GGATG 2 cut(s) 291, 406
BseLI CCNNNNNNNGG 2 cut(s) 783, 1405
BseMI GCAATG 2 cut(s) 72, 1110
BseMII CTCAG 4 cut(s) 490, 567, 1077, 1352
BseNI ACTGG 3 cut(s) 298, 360, 1415
BseRI GAGGAG 1 cut(s) 904
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 4 cut(s) 1018, 1231, 1279, 1301
BseYI CCCAGC 3 cut(s) 197, 395, 1027
Bsh1236I CGCG 1 cut(s) 1344
BshFI GGCC 1 cut(s) 895
BshNI GGYRCC 2 cut(s) 135, 1012
BshVI ATCGAT 1 cut(s) 1004
BsiHKAI GWGCWC 1 cut(s) 861
BsiSI CCGG 3 cut(s) 896, 966, 1280
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 2 cut(s) 783, 1405
BsmAI GTCTC 4 cut(s) 376, 454, 607, 1379
BsmBI CGTCTC 1 cut(s) 607
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 1 cut(s) 895
Bsp1286I GDGCHC 2 cut(s) 138, 861
Bsp13I TCCGGA 1 cut(s) 1279
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 7 cut(s) 109, 318, 468, 917, 1001, 1067, 1395
Bsp1720I GCTNAGC 1 cut(s) 1338
Bsp19I CCATGG 1 cut(s) 1401
BspACI CCGC 3 cut(s) 953, 1370, 1406
BspANI GGCC 1 cut(s) 895
BspCNI CTCAG 4 cut(s) 489, 568, 1076, 1351
BspDI ATCGAT 1 cut(s) 1004
BspEI TCCGGA 1 cut(s) 1279
BspFNI CGCG 1 cut(s) 1344
BspHI TCATGA 3 cut(s) 85, 853, 1381
BspMI ACCTGC 1 cut(s) 410
BspPI GGATC 6 cut(s) 313, 925, 1009, 1075, 1390, 1403
BspT107I GGYRCC 2 cut(s) 135, 1012
BsrDI GCAATG 2 cut(s) 72, 1110
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 3 cut(s) 298, 360, 1415
BssECI CCNNGG 6 cut(s) 139, 270, 1098, 1124, 1145, 1401
BssMI GATC 7 cut(s) 109, 318, 468, 917, 1001, 1067, 1395
BssNI GRCGYC 1 cut(s) 1047
BssT1I CCWWGG 3 cut(s) 270, 1098, 1401
Bst2UI CCWGG 4 cut(s) 29, 182, 1125, 1166
Bst4CI ACNGT 1 cut(s) 497
Bst6I CTCTTC 5 cut(s) 90, 100, 117, 293, 830
BstACI GRCGYC 1 cut(s) 1047
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 7 cut(s) 476, 576, 704, 1055, 1063, 1271, 1338
BstDSI CCRYGG 1 cut(s) 1401
BstF5I GGATG 2 cut(s) 291, 406
BstFNI CGCG 1 cut(s) 1344
BstHHI GCGC 1 cut(s) 1344
BstKTI GATC 7 cut(s) 112, 321, 471, 920, 1004, 1070, 1398
BstMAI GTCTC 4 cut(s) 376, 454, 607, 1379
BstMBI GATC 7 cut(s) 109, 318, 468, 917, 1001, 1067, 1395
BstMWI GCNNNNNNNGC 2 cut(s) 142, 1109
BstNI CCWGG 4 cut(s) 29, 182, 1125, 1166
BstNSI RCATGY 1 cut(s) 1022
BstSCI CCNGG 4 cut(s) 27, 180, 1123, 1164
BstSLI GKGCMC 1 cut(s) 138
BstUI CGCG 1 cut(s) 1344
BstV1I GCAGC 4 cut(s) 1018, 1231, 1279, 1301
BstX2I RGATCY 1 cut(s) 1395
BstYI RGATCY 1 cut(s) 1395
Bsu15I ATCGAT 1 cut(s) 1004
BsuRI GGCC 1 cut(s) 895
BsuTUI ATCGAT 1 cut(s) 1004
BtgI CCRYGG 1 cut(s) 1401
BtgZI GCGATG 1 cut(s) 131
BtsCI GGATG 2 cut(s) 291, 406
BveI ACCTGC 1 cut(s) 410
Cac8I GCNNGC 1 cut(s) 342
CciI TCATGA 3 cut(s) 85, 853, 1381
CfoI GCGC 1 cut(s) 1344
Cfr13I GGNCC 2 cut(s) 647, 1277
ClaI ATCGAT 1 cut(s) 1004
CseI GACGC 1 cut(s) 1036
Csp6I GTAC 6 cut(s) 301, 359, 406, 510, 557, 1150
CviAII CATG 9 cut(s) 86, 709, 783, 789, 854, 1019, 1382, 1391, 1402
CviQI GTAC 6 cut(s) 301, 359, 406, 510, 557, 1150
DdeI CTNAG 7 cut(s) 476, 576, 704, 1055, 1063, 1271, 1338
DpnI GATC 7 cut(s) 111, 320, 470, 919, 1003, 1069, 1397
DpnII GATC 7 cut(s) 109, 318, 468, 917, 1001, 1067, 1395
Eam1104I CTCTTC 5 cut(s) 90, 100, 117, 293, 830
EarI CTCTTC 5 cut(s) 90, 100, 117, 293, 830
Eco130I CCWWGG 3 cut(s) 270, 1098, 1401
Eco47I GGWCC 2 cut(s) 647, 1277
Eco57I CTGAAG 4 cut(s) 213, 449, 594, 1175
EcoRI GAATTC 1 cut(s) 411
EcoRII CCWGG 4 cut(s) 27, 180, 1123, 1164
EcoT14I CCWWGG 3 cut(s) 270, 1098, 1401
EcoT22I ATGCAT 1 cut(s) 595
ErhI CCWWGG 3 cut(s) 270, 1098, 1401
Esp3I CGTCTC 1 cut(s) 607
FaeI CATG 9 cut(s) 89, 712, 786, 792, 857, 1022, 1385, 1394, 1405
FaqI GGGAC 1 cut(s) 366
FatI CATG 9 cut(s) 85, 708, 782, 788, 853, 1018, 1381, 1390, 1401
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 4 cut(s) 1032, 1220, 1290, 1293
FokI GGATG 2 cut(s) 278, 413
Fsp4HI GCNGC 4 cut(s) 1032, 1220, 1290, 1293
FspBI CTAG 1 cut(s) 1251
GlaI GCGC 1 cut(s) 1343
GluI GCNGC 4 cut(s) 1032, 1220, 1290, 1293
GsaI CCCAGC 3 cut(s) 201, 399, 1031
GsuI CTGGAG 2 cut(s) 295, 1058
HaeIII GGCC 1 cut(s) 895
HapII CCGG 3 cut(s) 896, 966, 1280
HgaI GACGC 1 cut(s) 1036
HhaI GCGC 1 cut(s) 1344
Hin1I GRCGYC 1 cut(s) 1047
Hin1II CATG 9 cut(s) 89, 712, 786, 792, 857, 1022, 1385, 1394, 1405
Hin6I GCGC 1 cut(s) 1342
HinP1I GCGC 1 cut(s) 1342
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HinfI GANTC 4 cut(s) 308, 572, 938, 1321
HpaII CCGG 3 cut(s) 896, 966, 1280
HphI GGTGA 6 cut(s) 31, 196, 226, 259, 942, 1384
Hpy166II GTNNAC 6 cut(s) 361, 406, 751, 979, 1024, 1206
Hpy188III TCNNGA 8 cut(s) 86, 802, 854, 904, 1065, 1075, 1280, 1382
Hpy8I GTNNAC 6 cut(s) 361, 406, 751, 979, 1024, 1206
Hpy99I CGWCG 1 cut(s) 1052
HpyAV CCTTC 5 cut(s) 125, 385, 862, 1102, 1308
HpyCH4III ACNGT 1 cut(s) 497
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 5 cut(s) 166, 439, 593, 1034, 1289
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 1109
HpyF3I CTNAG 7 cut(s) 476, 576, 704, 1055, 1063, 1271, 1338
HpySE526I ACGT 1 cut(s) 600
Hsp92I GRCGYC 1 cut(s) 1047
Hsp92II CATG 9 cut(s) 89, 712, 786, 792, 857, 1022, 1385, 1394, 1405
HspAI GCGC 1 cut(s) 1342
Kpn2I TCCGGA 1 cut(s) 1279
Kzo9I GATC 7 cut(s) 109, 318, 468, 917, 1001, 1067, 1395
LmnI GCTCC 1 cut(s) 183
Lsp1109I GCAGC 4 cut(s) 1018, 1231, 1279, 1301
LweI GCATC 4 cut(s) 154, 489, 1181, 1387
MaeI CTAG 1 cut(s) 1251
MaeII ACGT 1 cut(s) 600
MalI GATC 7 cut(s) 111, 320, 470, 919, 1003, 1069, 1397
MboI GATC 7 cut(s) 109, 318, 468, 917, 1001, 1067, 1395
MboII GAAGA 9 cut(s) 101, 107, 117, 131, 134, 280, 517, 600, 847
MfeI CAATTG 2 cut(s) 386, 651
MflI RGATCY 1 cut(s) 1395
MhlI GDGCHC 2 cut(s) 138, 861
MluCI AATT 8 cut(s) 371, 386, 411, 520, 651, 667, 698, 1235
MlyI GAGTC 2 cut(s) 302, 947
Mph1103I ATGCAT 1 cut(s) 595
MroI TCCGGA 1 cut(s) 1279
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 4 cut(s) 81, 597, 993, 1332
MslI CAYNNNNRTG 3 cut(s) 90, 252, 787
MspA1I CMGCKG 2 cut(s) 1031, 1292
MspI CCGG 3 cut(s) 896, 966, 1280
MspR9I CCNGG 4 cut(s) 29, 182, 1125, 1166
MunI CAATTG 2 cut(s) 386, 651
MvaI CCWGG 4 cut(s) 29, 182, 1125, 1166
MvnI CGCG 1 cut(s) 1344
MwoI GCNNNNNNNGC 2 cut(s) 142, 1109
NcoI CCATGG 1 cut(s) 1401
NdeII GATC 7 cut(s) 109, 318, 468, 917, 1001, 1067, 1395
NlaIII CATG 9 cut(s) 89, 712, 786, 792, 857, 1022, 1385, 1394, 1405
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 1 cut(s) 1022
PagI TCATGA 3 cut(s) 85, 853, 1381
PciI ACATGT 1 cut(s) 1018
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 2 cut(s) 572, 1321
PkrI GCNGC 4 cut(s) 1033, 1221, 1291, 1294
PleI GAGTC 2 cut(s) 302, 946
PpsI GAGTC 2 cut(s) 302, 946
PscI ACATGT 1 cut(s) 1018
Psp6I CCWGG 4 cut(s) 27, 180, 1123, 1164
PspFI CCCAGC 3 cut(s) 197, 395, 1027
PspGI CCWGG 4 cut(s) 27, 180, 1123, 1164
PspPI GGNCC 2 cut(s) 647, 1277
PsuI RGATCY 1 cut(s) 1395
PvuII CAGCTG 2 cut(s) 1031, 1292
RsaI GTAC 6 cut(s) 302, 360, 407, 511, 558, 1151
RsaNI GTAC 6 cut(s) 301, 359, 406, 510, 557, 1150
RseI CAYNNNNRTG 3 cut(s) 90, 252, 787
SalI GTCGAC 1 cut(s) 749
SaqAI TTAA 4 cut(s) 81, 597, 993, 1332
SatI GCNGC 4 cut(s) 1032, 1220, 1290, 1293
Sau3AI GATC 7 cut(s) 109, 318, 468, 917, 1001, 1067, 1395
Sau96I GGNCC 2 cut(s) 647, 1277
SchI GAGTC 2 cut(s) 302, 947
ScrFI CCNGG 4 cut(s) 29, 182, 1125, 1166
SduI GDGCHC 2 cut(s) 138, 861
SfaNI GCATC 4 cut(s) 154, 489, 1181, 1387
SinI GGWCC 2 cut(s) 647, 1277
SmiMI CAYNNNNRTG 3 cut(s) 90, 252, 787
Sse9I AATT 8 cut(s) 371, 386, 411, 520, 651, 667, 698, 1235
SsiI CCGC 3 cut(s) 953, 1370, 1406
SspMI CTAG 1 cut(s) 1251
StyD4I CCNGG 4 cut(s) 27, 180, 1123, 1164
StyI CCWWGG 3 cut(s) 270, 1098, 1401
TaaI ACNGT 1 cut(s) 497
TaiI ACGT 1 cut(s) 603
TaqI TCGA 7 cut(s) 306, 483, 570, 750, 825, 920, 1004
TasI AATT 8 cut(s) 371, 386, 411, 520, 651, 667, 698, 1235
TatI WGTACW 2 cut(s) 405, 556
TfiI GAWTC 2 cut(s) 572, 1321
Tru1I TTAA 4 cut(s) 81, 597, 993, 1332
Tru9I TTAA 4 cut(s) 81, 597, 993, 1332
TseI GCWGC 4 cut(s) 1031, 1219, 1289, 1292
TspDTI ATGAA 6 cut(s) 102, 108, 280, 517, 725, 1121
TspGWI ACGGA 1 cut(s) 1107
VpaK11BI GGWCC 2 cut(s) 647, 1277
XapI RAATTY 3 cut(s) 411, 520, 698
XceI RCATGY 1 cut(s) 1022
XmiI GTMKAC 1 cut(s) 750
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 1 cut(s) 1251
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.