Rw5G037200

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0001754
Physical Location & Seq
Reverse (-)
65529540 .. 65531647
2108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G037200.1

Sequence Viewer

Length: 1380 bp
ATGGGAAACAGAGTGATGGTGAGCCTCCTGATTCTTGTGCTTGTGCTTTCCTATGGAGTTATGGCAATGGCTATGAGGTTTAATCATGAAGATGAAGAGTGGGGAAGAGATCAGAGAGAAGAAGAGTGGGAAGGGCACCGAGGGCATCGCCATCTGTTCCTTTTGCAACAATCAAGGCTCCTGGTGAAAACTGAAGCTGGGCAAATGAGGGTGGTGAGAAGTGCAGGTCAGAGAGTGGTAGATAGGCATATAAATGTTGCCTATCTCACCTTGGAACCTAACTCCCTCTTCATCCCCCAGTACCTCGACTCCAGCTTGATCCTCTTTGTCCAAAGAGGGCAAGCAAATGTGGGACTGGTACACCGACAGGAATTGGGGGAGAGACAATTGAAGGCTGGGGATGTGTACAGAATTCCAGCAGGTTCCACATTTTACTTGCAGAATGTCAGGGAGAACAGGAGACTTCAGATCATTCTCAGCATCGACACATCTGACAGTTTGAGAATGGGTACTTTGGAGAATTTCTTCATTGGTGGAGGAACCTACCCAAAATCTGTACTTTCTGGGTTCGATTCTGAGATACTCACAAATGCATTTAATGTCTCATCTTCAGAAATAAGGCAGGTTTTGACAAGGCAACAAGAGGGTCCAATTATATATCTAAATAATTCCCAATCATCATCAAACCTTTGGACCAAATTCGTAAGCATGAAAGAGCAGGACAGACTACAAGAATTGAAGAAAATGGTGGACTTCCCAGAAGAATCAGAACAAACCCTAACTTGGTCATGGAGGAAACTGTTGAACTCCGTGTTCGGAACACTTGAAAATGAGGAGAGGAAGCAACATCATCATGAGCACTCCCAGAAATCCTACAACCTCTATGATAGAAAGCCAGATTTCAAGAACAACCATGGATGGACCCTCTCAGTTGATGAGTCTGATTACTCTCCCCTAAAGGACTCCGGCATTGGCGTTTACCTCGTTAGTCTCAACGCAGGGTCAATGTTGGCACCACATGTGAACCCAAGAGCAACAGAATACGGCGTCGTTCTGAGAGGCTCAGGATCACTGCAGATTGTATTTCCAAATGCCAGGGAGAGTGTTTGGGTGCCAAGGTATTTCCCATTTGTCCAAGTGGCATCTAACGACGAGCAACTTGAGATTTTTGGGTTCACTACCTCAGCACAGCCGAACCGGCCACAGTTTTTGGCTGGTTCTAGCTCGGTGCTGCAAGCTCTAAGAGGTCAAGAACTTGCAGCTGCTTTTGGTGTGAGTGAGGGAAGGTTGAATCATTTTGTTAATGCTCAGCGCGAAGCTGTCATTTTGCCATACAATGCAGAGAGGAATCAAAAGGCATATGGGATGGCAGACCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

459

Amino Acids

52.3

Weight (kDa)

6.69

Isoelectric Point (pI)

44.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 54 - 206 1.4e-12 Cupin
Cupin_1 PF00190 292 - 427 5.1e-24 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 3 cut(s) 215, 410, 613
AccB1I GGYRCC 3 cut(s) 135, 1012, 1111
AccII CGCG 1 cut(s) 1314
AclWI GGATC 2 cut(s) 313, 1075
AcoI YGGCCR 1 cut(s) 1199
AcsI RAATTY 3 cut(s) 411, 520, 698
AcuI CTGAAG 3 cut(s) 213, 449, 594
AcyI GRCGYC 1 cut(s) 1047
AfaI GTAC 5 cut(s) 302, 360, 407, 511, 558
AfiI CCNNNNNNNGG 1 cut(s) 783
AflIII ACRYGT 1 cut(s) 1018
AgeI ACCGGT 1 cut(s) 1374
AgsI TTSAA 6 cut(s) 391, 739, 805, 827, 904, 1291
AjnI CCWGG 2 cut(s) 180, 1094
AluBI AGCT 6 cut(s) 197, 315, 1224, 1238, 1262, 1319
AluI AGCT 6 cut(s) 197, 315, 1224, 1238, 1262, 1319
Alw21I GWGCWC 1 cut(s) 861
Alw26I GTCTC 4 cut(s) 376, 454, 607, 995
AlwI GGATC 2 cut(s) 313, 1075
AoxI GGCC 1 cut(s) 1199
ApeKI GCWGC 3 cut(s) 1231, 1259, 1262
ApoI RAATTY 3 cut(s) 411, 520, 698
AsiGI ACCGGT 1 cut(s) 1374
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 1 cut(s) 1314
AspS9I GGNCC 3 cut(s) 647, 693, 921
AsuHPI GGTGA 4 cut(s) 31, 196, 226, 259
AvaII GGWCC 3 cut(s) 647, 693, 921
BaeGI GKGCMC 1 cut(s) 138
BanI GGYRCC 3 cut(s) 135, 1012, 1111
Bbv12I GWGCWC 1 cut(s) 861
BbvCI CCTCAGC 1 cut(s) 1183
BbvI GCAGC 3 cut(s) 1218, 1249, 1271
BccI CCATC 4 cut(s) 10, 159, 912, 1360
BceAI ACGGC 1 cut(s) 1060
BciT130I CCWGG 2 cut(s) 182, 1096
BcoDI GTCTC 4 cut(s) 376, 454, 607, 995
BfaI CTAG 1 cut(s) 1221
BfmI CTRYAG 1 cut(s) 1073
BfuAI ACCTGC 3 cut(s) 215, 410, 613
BglI GCCNNNNNGGC 1 cut(s) 1198
BisI GCNGC 3 cut(s) 1232, 1260, 1263
BlpI GCTNAGC 1 cut(s) 1308
BlsI GCNGC 3 cut(s) 1233, 1261, 1264
Bme1390I CCNGG 2 cut(s) 182, 1096
Bme18I GGWCC 3 cut(s) 647, 693, 921
BmgT120I GGNCC 3 cut(s) 647, 693, 921
BmiI GGNNCC 9 cut(s) 137, 179, 276, 424, 541, 648, 923, 1014, 1113
BmrFI CCNGG 2 cut(s) 182, 1096
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 3 cut(s) 154, 489, 1151
BmuI ACTGGG 1 cut(s) 292
BpmI CTGGAG 1 cut(s) 295
Bpu10I CCTNAGC 2 cut(s) 1063, 1183
Bpu1102I GCTNAGC 1 cut(s) 1308
BpuEI CTTGAG 1 cut(s) 1181
BsaHI GRCGYC 1 cut(s) 1047
BsaJI CCNNGG 5 cut(s) 139, 270, 913, 1095, 1115
BsaWI WCCGGW 1 cut(s) 1374
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 1 cut(s) 783
Bse118I RCCGGY 2 cut(s) 1197, 1374
Bse1I ACTGG 2 cut(s) 298, 360
Bse3DI GCAATG 1 cut(s) 72
BseBI CCWGG 2 cut(s) 182, 1096
BseDI CCNNGG 5 cut(s) 139, 270, 913, 1095, 1115
BseGI GGATG 4 cut(s) 291, 406, 923, 1371
BseLI CCNNNNNNNGG 1 cut(s) 783
BseMI GCAATG 1 cut(s) 72
BseMII CTCAG 7 cut(s) 490, 567, 942, 1046, 1077, 1197, 1322
BseNI ACTGG 2 cut(s) 298, 360
BseRI GAGGAG 1 cut(s) 848
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 3 cut(s) 1218, 1249, 1271
BseYI CCCAGC 2 cut(s) 197, 395
BsgI GTGCAG 1 cut(s) 243
Bsh1236I CGCG 1 cut(s) 1314
BshFI GGCC 1 cut(s) 1201
BshNI GGYRCC 3 cut(s) 135, 1012, 1111
BshTI ACCGGT 1 cut(s) 1374
BsiHKAI GWGCWC 1 cut(s) 861
BsiSI CCGG 3 cut(s) 966, 1198, 1375
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 1 cut(s) 783
BsmAI GTCTC 4 cut(s) 376, 454, 607, 995
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 1 cut(s) 1201
Bsp1286I GDGCHC 2 cut(s) 138, 861
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 4 cut(s) 109, 318, 468, 1067
Bsp1720I GCTNAGC 1 cut(s) 1308
Bsp19I CCATGG 1 cut(s) 913
BspANI GGCC 1 cut(s) 1201
BspCNI CTCAG 7 cut(s) 489, 568, 941, 1047, 1076, 1196, 1321
BspFNI CGCG 1 cut(s) 1314
BspHI TCATGA 2 cut(s) 85, 853
BspLI GGNNCC 9 cut(s) 137, 179, 276, 424, 541, 648, 923, 1014, 1113
BspMAI CTGCAG 1 cut(s) 1077
BspMI ACCTGC 3 cut(s) 215, 410, 613
BspPI GGATC 2 cut(s) 313, 1075
BspT107I GGYRCC 3 cut(s) 135, 1012, 1111
BsrDI GCAATG 1 cut(s) 72
BsrFI RCCGGY 2 cut(s) 1197, 1374
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 2 cut(s) 298, 360
BssAI RCCGGY 2 cut(s) 1197, 1374
BssECI CCNNGG 5 cut(s) 139, 270, 913, 1095, 1115
BssMI GATC 4 cut(s) 109, 318, 468, 1067
BssNI GRCGYC 1 cut(s) 1047
BssT1I CCWWGG 3 cut(s) 270, 913, 1115
Bst2UI CCWGG 2 cut(s) 182, 1096
Bst4CI ACNGT 3 cut(s) 497, 801, 1206
Bst6I CTCTTC 4 cut(s) 90, 100, 117, 293
BstACI GRCGYC 1 cut(s) 1047
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 2 cut(s) 342, 1236
BstDEI CTNAG 8 cut(s) 476, 576, 928, 1055, 1063, 1183, 1241, 1308
BstDSI CCRYGG 1 cut(s) 913
BstF5I GGATG 4 cut(s) 291, 406, 923, 1371
BstFNI CGCG 1 cut(s) 1314
BstHHI GCGC 1 cut(s) 1314
BstKTI GATC 4 cut(s) 112, 321, 471, 1070
BstMAI GTCTC 4 cut(s) 376, 454, 607, 995
BstMBI GATC 4 cut(s) 109, 318, 468, 1067
BstMWI GCNNNNNNNGC 2 cut(s) 142, 1198
BstNI CCWGG 2 cut(s) 182, 1096
BstNSI RCATGY 1 cut(s) 1022
BstSCI CCNGG 2 cut(s) 180, 1094
BstSFI CTRYAG 1 cut(s) 1073
BstSLI GKGCMC 1 cut(s) 138
BstUI CGCG 1 cut(s) 1314
BstV1I GCAGC 3 cut(s) 1218, 1249, 1271
BsuRI GGCC 1 cut(s) 1201
BtgI CCRYGG 1 cut(s) 913
BtgZI GCGATG 1 cut(s) 131
BtsCI GGATG 4 cut(s) 291, 406, 923, 1371
BtsI GCAGTG 1 cut(s) 1070
BtsIMutI CAGTG 1 cut(s) 1070
BveI ACCTGC 3 cut(s) 215, 410, 613
Cac8I GCNNGC 2 cut(s) 342, 1236
CciI TCATGA 2 cut(s) 85, 853
CfoI GCGC 1 cut(s) 1314
Cfr10I RCCGGY 2 cut(s) 1197, 1374
Cfr13I GGNCC 3 cut(s) 647, 693, 921
CseI GACGC 1 cut(s) 1036
Csp6I GTAC 5 cut(s) 301, 359, 406, 510, 557
CspAI ACCGGT 1 cut(s) 1374
CspCI CAANNNNNGTGG 2 cut(s) 1191, 1226
CviAII CATG 6 cut(s) 86, 709, 789, 854, 914, 1019
CviQI GTAC 5 cut(s) 301, 359, 406, 510, 557
DdeI CTNAG 8 cut(s) 476, 576, 928, 1055, 1063, 1183, 1241, 1308
DpnI GATC 4 cut(s) 111, 320, 470, 1069
DpnII GATC 4 cut(s) 109, 318, 468, 1067
EaeI YGGCCR 1 cut(s) 1199
Eam1104I CTCTTC 4 cut(s) 90, 100, 117, 293
EarI CTCTTC 4 cut(s) 90, 100, 117, 293
Eco130I CCWWGG 3 cut(s) 270, 913, 1115
Eco47I GGWCC 3 cut(s) 647, 693, 921
Eco57I CTGAAG 3 cut(s) 213, 449, 594
EcoRI GAATTC 1 cut(s) 411
EcoRII CCWGG 2 cut(s) 180, 1094
EcoT14I CCWWGG 3 cut(s) 270, 913, 1115
EcoT22I ATGCAT 1 cut(s) 595
ErhI CCWWGG 3 cut(s) 270, 913, 1115
FaeI CATG 6 cut(s) 89, 712, 792, 857, 917, 1022
FaqI GGGAC 1 cut(s) 366
FatI CATG 6 cut(s) 85, 708, 788, 853, 913, 1018
FauNDI CATATG 1 cut(s) 1360
Fnu4HI GCNGC 3 cut(s) 1232, 1260, 1263
FokI GGATG 3 cut(s) 278, 413, 930
Fsp4HI GCNGC 3 cut(s) 1232, 1260, 1263
FspBI CTAG 1 cut(s) 1221
GlaI GCGC 1 cut(s) 1313
GluI GCNGC 3 cut(s) 1232, 1260, 1263
GsaI CCCAGC 2 cut(s) 201, 399
GsuI CTGGAG 1 cut(s) 295
HaeIII GGCC 1 cut(s) 1201
HapII CCGG 3 cut(s) 966, 1198, 1375
HgaI GACGC 1 cut(s) 1036
HhaI GCGC 1 cut(s) 1314
Hin1I GRCGYC 1 cut(s) 1047
Hin1II CATG 6 cut(s) 89, 712, 792, 857, 917, 1022
Hin6I GCGC 1 cut(s) 1312
HinP1I GCGC 1 cut(s) 1312
HinfI GANTC 8 cut(s) 31, 308, 572, 764, 938, 962, 1291, 1348
HpaII CCGG 3 cut(s) 966, 1198, 1375
HphI GGTGA 4 cut(s) 31, 196, 226, 259
Hpy166II GTNNAC 6 cut(s) 361, 406, 751, 979, 1024, 1176
Hpy188III TCNNGA 6 cut(s) 28, 86, 854, 904, 1065, 1250
Hpy8I GTNNAC 6 cut(s) 361, 406, 751, 979, 1024, 1176
Hpy99I CGWCG 2 cut(s) 1052, 1154
HpyAV CCTTC 3 cut(s) 125, 385, 1278
HpyCH4III ACNGT 3 cut(s) 497, 801, 1206
HpyCH4V TGCA 8 cut(s) 166, 224, 439, 593, 1075, 1234, 1259, 1340
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 1198
HpyF3I CTNAG 8 cut(s) 476, 576, 928, 1055, 1063, 1183, 1241, 1308
Hsp92I GRCGYC 1 cut(s) 1047
Hsp92II CATG 6 cut(s) 89, 712, 792, 857, 917, 1022
HspAI GCGC 1 cut(s) 1312
Kzo9I GATC 4 cut(s) 109, 318, 468, 1067
LmnI GCTCC 1 cut(s) 183
Lsp1109I GCAGC 3 cut(s) 1218, 1249, 1271
LweI GCATC 3 cut(s) 154, 489, 1151
MaeI CTAG 1 cut(s) 1221
MalI GATC 4 cut(s) 111, 320, 470, 1069
MboI GATC 4 cut(s) 109, 318, 468, 1067
MfeI CAATTG 1 cut(s) 386
MhlI GDGCHC 2 cut(s) 138, 861
MluCI AATT 8 cut(s) 371, 386, 411, 520, 651, 667, 698, 734
MlyI GAGTC 3 cut(s) 302, 947, 956
Mph1103I ATGCAT 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 3 cut(s) 81, 597, 1302
MslI CAYNNNNRTG 3 cut(s) 90, 252, 852
MspA1I CMGCKG 1 cut(s) 1262
MspI CCGG 3 cut(s) 966, 1198, 1375
MspR9I CCNGG 2 cut(s) 182, 1096
MunI CAATTG 1 cut(s) 386
MvaI CCWGG 2 cut(s) 182, 1096
MvnI CGCG 1 cut(s) 1314
MwoI GCNNNNNNNGC 2 cut(s) 142, 1198
NcoI CCATGG 1 cut(s) 913
NdeI CATATG 1 cut(s) 1360
NdeII GATC 4 cut(s) 109, 318, 468, 1067
NlaIII CATG 6 cut(s) 89, 712, 792, 857, 917, 1022
NlaIV GGNNCC 9 cut(s) 137, 179, 276, 424, 541, 648, 923, 1014, 1113
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 1 cut(s) 1022
PagI TCATGA 2 cut(s) 85, 853
PciI ACATGT 1 cut(s) 1018
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 5 cut(s) 31, 572, 764, 1291, 1348
PinAI ACCGGT 1 cut(s) 1374
PkrI GCNGC 3 cut(s) 1233, 1261, 1264
PleI GAGTC 3 cut(s) 302, 946, 956
PpsI GAGTC 3 cut(s) 302, 946, 956
PscI ACATGT 1 cut(s) 1018
Psp6I CCWGG 2 cut(s) 180, 1094
PspFI CCCAGC 2 cut(s) 197, 395
PspGI CCWGG 2 cut(s) 180, 1094
PspN4I GGNNCC 9 cut(s) 137, 179, 276, 424, 541, 648, 923, 1014, 1113
PspPI GGNCC 3 cut(s) 647, 693, 921
PstI CTGCAG 1 cut(s) 1077
PvuII CAGCTG 1 cut(s) 1262
RsaI GTAC 5 cut(s) 302, 360, 407, 511, 558
RsaNI GTAC 5 cut(s) 301, 359, 406, 510, 557
RseI CAYNNNNRTG 3 cut(s) 90, 252, 852
SaqAI TTAA 3 cut(s) 81, 597, 1302
SatI GCNGC 3 cut(s) 1232, 1260, 1263
Sau3AI GATC 4 cut(s) 109, 318, 468, 1067
Sau96I GGNCC 3 cut(s) 647, 693, 921
SchI GAGTC 3 cut(s) 302, 947, 956
ScrFI CCNGG 2 cut(s) 182, 1096
SduI GDGCHC 2 cut(s) 138, 861
SfaNI GCATC 3 cut(s) 154, 489, 1151
SfcI CTRYAG 1 cut(s) 1073
SinI GGWCC 3 cut(s) 647, 693, 921
SmiMI CAYNNNNRTG 3 cut(s) 90, 252, 852
SmlI CTYRAG 1 cut(s) 1160
SmoI CTYRAG 1 cut(s) 1160
Sse9I AATT 8 cut(s) 371, 386, 411, 520, 651, 667, 698, 734
SspMI CTAG 1 cut(s) 1221
StyD4I CCNGG 2 cut(s) 180, 1094
StyI CCWWGG 3 cut(s) 270, 913, 1115
TaaI ACNGT 3 cut(s) 497, 801, 1206
TaqI TCGA 3 cut(s) 306, 483, 570
TasI AATT 8 cut(s) 371, 386, 411, 520, 651, 667, 698, 734
TatI WGTACW 2 cut(s) 405, 556
TfiI GAWTC 5 cut(s) 31, 572, 764, 1291, 1348
Tru1I TTAA 3 cut(s) 81, 597, 1302
Tru9I TTAA 3 cut(s) 81, 597, 1302
TscAI CASTG 1 cut(s) 1077
TseI GCWGC 3 cut(s) 1231, 1259, 1262
TspDTI ATGAA 5 cut(s) 102, 108, 280, 517, 725
TspGWI ACGGA 1 cut(s) 799
TspRI CASTG 1 cut(s) 1077
VpaK11BI GGWCC 3 cut(s) 647, 693, 921
XapI RAATTY 3 cut(s) 411, 520, 698
XceI RCATGY 1 cut(s) 1022
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 1 cut(s) 1221
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.