Rh5DG422500

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001754
Physical Location & Seq
Reverse (-)
67360976 .. 67411939
50964 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG422500.1

Sequence Viewer

Length: 1416 bp
ATGGGAAAGAAAGTGATCGTAAGCGTCCTAGTTCTTGCACTTGTGATGTGCTTTGGAGTCATGGCAATGGCTATGAGCTTCGATCACGAAGACGAGGACTGGGGATGGGATCAGAGAGAAGAAGAGTGGGAAGGGCACAAAGGGCATCGCCATTGGTTCCTTTTGCAACGATCTAGACTACTGCTGAGAACTGAAGCTGGGGAAATGAGGGTGGTGAGGAGTGTAGGTCGGAGAATGGTCAATAGACATATAAATGTTGGGTTTCTCTCCATGAAACCCAACTCTCTTTTCATCCCTCAGTACCTTGACTCCAGCGTGGTCCTCTTTGTCCACACAGGGCAAGCAAATGTGGGACTAGTGCACCGACATGAACTCGGAGAGAGGCAATTAAAGGCTGGGGATTTGTACAGAATCCCAGCTGGTTCAACATTTTACTTGCAGAATGTTGGGGAGGGCCAGAGACTTGAGCTCATTCTCAGCATTGACACATCTGACAGTTTGAGAATGGGTACTTTGGAGAATTTCTTCATTGGTGGAGGAAACTACCCGAAATCTGTCATTGCTGGGTTTGACTCTGAAATACTCAGAAATGCATTTAACGTCTCATCTTCAGAACTAAGGCAGGTTTTGACAAGGCAACAAGAGGGTCCAATTATATCTTTGAATAATTCTCAGTCATCATCAAACCTTTGGACAAAATTCCTAAGCATGAAAGAGCAGGACAGACTACAAGAACTAAAGAAAATGGTCGACTTCCCACAAGAACAAGAGCAAACCCAAACATGGTCATGGAGGAAACTCTTGAACTCTATGTTCGGAACAATCGAAAAAGAGAAGAGAAAGCAGAATCATCATGAGCACTCCCAGGAGGCTTACAACCTCTATGAGAGGAGGCCAGATTTCAAGAACAACTATGGATCAAGCTTGGCAGTTGATGAGTCTGATTACTCACCGCTACAAAACTCCGGCATTGGCGTTTACCTTGTCAATCTTAACGCAGGATCGATGTTGGCACCACATGTGAACCCAACAGCAACAGAATACGGCGTCGTTCTAAGAGGCTCGGGATCACTCCAGATTGTGTTTCCAAATGGAACCTTGGCAATGAAGGCCAAACTAAAACCAGGGAGAGTGTTCTGGGTGCCTAGGTACTTCCCATTTGTCCAAGTGGCATCTAACAACGAAAAACTTGAGATTTTTGGATTCACTACCTCGGCACAGCCGAACCGGCCACAGTTTTTGGCCGGTTCTAGCTCGGTGCTTGAAGCTCTAAGAGGTCCGGAACTTGCAGCTGCTTTTGGTGTGAGTGAGGAAAGGTTGAATCGTTTTGTTAATGCTCAGCGCGAAGCTGTAATCTTGCCTTACAATGCGGAGAGGCATCATGAGACACATGGGATCCCCATGGCGGACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

471

Amino Acids

53.47

Weight (kDa)

7.3

Isoelectric Point (pI)

44.94

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 83 - 205 1.1e-08 Cupin
Cupin_1 PF00190 292 - 438 5.1e-32 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 613
AccB1I GGYRCC 2 cut(s) 1012, 1141
AccI GTMKAC 1 cut(s) 750
AccII CGCG 1 cut(s) 1344
AccIII TCCGGA 1 cut(s) 1279
AciI CCGC 3 cut(s) 953, 1370, 1406
AclWI GGATC 6 cut(s) 117, 925, 1009, 1075, 1390, 1403
AcoI YGGCCR 2 cut(s) 1229, 1242
AcsI RAATTY 2 cut(s) 520, 698
AcuI CTGAAG 2 cut(s) 213, 594
AcyI GRCGYC 1 cut(s) 1047
AfaI GTAC 4 cut(s) 302, 407, 511, 1151
AfiI CCNNNNNNNGG 2 cut(s) 783, 1405
AflIII ACRYGT 1 cut(s) 1018
AgsI TTSAA 6 cut(s) 426, 664, 805, 904, 1265, 1321
AhlI ACTAGT 1 cut(s) 355
AjnI CCWGG 2 cut(s) 864, 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
AluBI AGCT 9 cut(s) 78, 197, 419, 469, 924, 1254, 1268, 1292, 1349
AluI AGCT 9 cut(s) 78, 197, 419, 469, 924, 1254, 1268, 1292, 1349
Alw21I GWGCWC 3 cut(s) 363, 471, 861
Alw26I GTCTC 3 cut(s) 454, 607, 1379
Alw44I GTGCAC 1 cut(s) 359
AlwI GGATC 6 cut(s) 117, 925, 1009, 1075, 1390, 1403
Ama87I CYCGRG 1 cut(s) 1063
Aor13HI TCCGGA 1 cut(s) 1279
AoxI GGCC 5 cut(s) 454, 893, 1110, 1229, 1242
ApaLI GTGCAC 1 cut(s) 359
ApeKI GCWGC 2 cut(s) 1289, 1292
ApoI RAATTY 2 cut(s) 520, 698
Asp700I GAANNNNTTC 1 cut(s) 524
AspA2I CCTAGG 1 cut(s) 1145
AspLEI GCGC 1 cut(s) 1344
AspS9I GGNCC 4 cut(s) 319, 454, 647, 1277
AsuHPI GGTGA 2 cut(s) 226, 942
AvaI CYCGRG 1 cut(s) 1063
AvaII GGWCC 3 cut(s) 319, 647, 1277
AvrII CCTAGG 1 cut(s) 1145
BaeGI GKGCMC 2 cut(s) 138, 363
BamHI GGATCC 1 cut(s) 1395
BanI GGYRCC 2 cut(s) 1012, 1141
BanII GRGCYC 1 cut(s) 471
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 3 cut(s) 363, 471, 861
BbvI GCAGC 2 cut(s) 1279, 1301
BccI CCATC 1 cut(s) 99
BceAI ACGGC 1 cut(s) 1060
BciT130I CCWGG 2 cut(s) 866, 1125
BcoDI GTCTC 3 cut(s) 454, 607, 1379
BcuI ACTAGT 1 cut(s) 355
BfaI CTAG 5 cut(s) 29, 174, 356, 1146, 1251
BfuAI ACCTGC 1 cut(s) 613
BglI GCCNNNNNGGC 1 cut(s) 1228
BisI GCNGC 2 cut(s) 1290, 1293
BlnI CCTAGG 1 cut(s) 1145
BlpI GCTNAGC 1 cut(s) 1338
BlsI GCNGC 2 cut(s) 1291, 1294
Bme1390I CCNGG 2 cut(s) 866, 1125
Bme18I GGWCC 3 cut(s) 319, 647, 1277
BmeT110I CYCGRG 1 cut(s) 1063
BmgT120I GGNCC 4 cut(s) 319, 454, 647, 1277
BmiI GGNNCC 6 cut(s) 158, 648, 1014, 1096, 1143, 1397
BmrFI CCNGG 2 cut(s) 866, 1125
BmrI ACTGGG 1 cut(s) 109
BmsI GCATC 3 cut(s) 154, 1181, 1387
BmuI ACTGGG 1 cut(s) 109
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 2 cut(s) 295, 1058
Bpu10I CCTNAGC 1 cut(s) 704
Bpu1102I GCTNAGC 1 cut(s) 1338
BpuEI CTTGAG 2 cut(s) 485, 1211
Bsa29I ATCGAT 1 cut(s) 1004
BsaHI GRCGYC 1 cut(s) 1047
BsaJI CCNNGG 6 cut(s) 864, 1098, 1124, 1145, 1212, 1401
BsaWI WCCGGW 1 cut(s) 1279
BsaXI ACNNNNNCTCC 4 cut(s) 211, 241, 293, 323
Bsc4I CCNNNNNNNGG 2 cut(s) 783, 1405
Bse118I RCCGGY 2 cut(s) 1227, 1244
Bse1I ACTGG 2 cut(s) 104, 1415
Bse3DI GCAATG 3 cut(s) 72, 558, 1110
BseAI TCCGGA 1 cut(s) 1279
BseBI CCWGG 2 cut(s) 866, 1125
BseCI ATCGAT 1 cut(s) 1004
BseDI CCNNGG 6 cut(s) 864, 1098, 1124, 1145, 1212, 1401
BseGI GGATG 2 cut(s) 110, 291
BseLI CCNNNNNNNGG 2 cut(s) 783, 1405
BseMI GCAATG 3 cut(s) 72, 558, 1110
BseMII CTCAG 6 cut(s) 176, 311, 490, 598, 686, 1352
BseNI ACTGG 2 cut(s) 104, 1415
BseRI GAGGAG 2 cut(s) 232, 904
BseSI GKGCMC 2 cut(s) 138, 363
BseXI GCAGC 2 cut(s) 1279, 1301
BseYI CCCAGC 4 cut(s) 197, 395, 415, 563
Bsh1236I CGCG 1 cut(s) 1344
BshFI GGCC 5 cut(s) 456, 895, 1112, 1231, 1244
BshNI GGYRCC 2 cut(s) 1012, 1141
BshVI ATCGAT 1 cut(s) 1004
BsiHKAI GWGCWC 3 cut(s) 363, 471, 861
BsiHKCI CYCGRG 1 cut(s) 1063
BsiSI CCGG 4 cut(s) 966, 1228, 1245, 1280
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 2 cut(s) 783, 1405
BsmAI GTCTC 3 cut(s) 454, 607, 1379
BsmBI CGTCTC 1 cut(s) 607
BsmFI GGGAC 1 cut(s) 366
BsnI GGCC 5 cut(s) 456, 895, 1112, 1231, 1244
BsoBI CYCGRG 1 cut(s) 1063
Bsp1286I GDGCHC 4 cut(s) 138, 363, 471, 861
Bsp13I TCCGGA 1 cut(s) 1279
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 8 cut(s) 15, 82, 109, 170, 917, 1001, 1067, 1395
Bsp1720I GCTNAGC 1 cut(s) 1338
Bsp19I CCATGG 1 cut(s) 1401
BspACI CCGC 3 cut(s) 953, 1370, 1406
BspANI GGCC 5 cut(s) 456, 895, 1112, 1231, 1244
BspCNI CTCAG 6 cut(s) 177, 310, 489, 597, 685, 1351
BspDI ATCGAT 1 cut(s) 1004
BspEI TCCGGA 1 cut(s) 1279
BspFNI CGCG 1 cut(s) 1344
BspHI TCATGA 2 cut(s) 853, 1381
BspLI GGNNCC 6 cut(s) 158, 648, 1014, 1096, 1143, 1397
BspMI ACCTGC 1 cut(s) 613
BspPI GGATC 6 cut(s) 117, 925, 1009, 1075, 1390, 1403
BspT107I GGYRCC 2 cut(s) 1012, 1141
BsrDI GCAATG 3 cut(s) 72, 558, 1110
BsrFI RCCGGY 2 cut(s) 1227, 1244
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 2 cut(s) 104, 1415
BssAI RCCGGY 2 cut(s) 1227, 1244
BssECI CCNNGG 6 cut(s) 864, 1098, 1124, 1145, 1212, 1401
BssMI GATC 8 cut(s) 15, 82, 109, 170, 917, 1001, 1067, 1395
BssNI GRCGYC 1 cut(s) 1047
BssT1I CCWWGG 3 cut(s) 1098, 1145, 1401
Bst2UI CCWGG 2 cut(s) 866, 1125
Bst4CI ACNGT 2 cut(s) 497, 1236
Bst6I CTCTTC 2 cut(s) 117, 830
BstACI GRCGYC 1 cut(s) 1047
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 342
BstDSI CCRYGG 1 cut(s) 1401
BstF5I GGATG 2 cut(s) 110, 291
BstFNI CGCG 1 cut(s) 1344
BstHHI GCGC 1 cut(s) 1344
BstKTI GATC 8 cut(s) 18, 85, 112, 173, 920, 1004, 1070, 1398
BstMAI GTCTC 3 cut(s) 454, 607, 1379
BstMBI GATC 8 cut(s) 15, 82, 109, 170, 917, 1001, 1067, 1395
BstMWI GCNNNNNNNGC 3 cut(s) 142, 1109, 1228
BstNI CCWGG 2 cut(s) 866, 1125
BstNSI RCATGY 1 cut(s) 1022
BstSCI CCNGG 2 cut(s) 864, 1123
BstSLI GKGCMC 2 cut(s) 138, 363
BstUI CGCG 1 cut(s) 1344
BstV1I GCAGC 2 cut(s) 1279, 1301
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 1395
BstYI RGATCY 1 cut(s) 1395
Bsu15I ATCGAT 1 cut(s) 1004
BsuRI GGCC 5 cut(s) 456, 895, 1112, 1231, 1244
BsuTUI ATCGAT 1 cut(s) 1004
BtgI CCRYGG 1 cut(s) 1401
BtgZI GCGATG 1 cut(s) 131
BtsCI GGATG 2 cut(s) 110, 291
BveI ACCTGC 1 cut(s) 613
Cac8I GCNNGC 1 cut(s) 342
CciI TCATGA 2 cut(s) 853, 1381
CfoI GCGC 1 cut(s) 1344
Cfr10I RCCGGY 2 cut(s) 1227, 1244
Cfr13I GGNCC 4 cut(s) 319, 454, 647, 1277
ClaI ATCGAT 1 cut(s) 1004
CseI GACGC 2 cut(s) 13, 1036
Csp6I GTAC 4 cut(s) 301, 406, 510, 1150
CspCI CAANNNNNGTGG 2 cut(s) 1221, 1256
CviQI GTAC 4 cut(s) 301, 406, 510, 1150
DpnI GATC 8 cut(s) 17, 84, 111, 172, 919, 1003, 1069, 1397
DpnII GATC 8 cut(s) 15, 82, 109, 170, 917, 1001, 1067, 1395
EaeI YGGCCR 2 cut(s) 1229, 1242
Eam1104I CTCTTC 2 cut(s) 117, 830
EarI CTCTTC 2 cut(s) 117, 830
Ecl136II GAGCTC 1 cut(s) 469
Eco130I CCWWGG 3 cut(s) 1098, 1145, 1401
Eco24I GRGCYC 1 cut(s) 471
Eco47I GGWCC 3 cut(s) 319, 647, 1277
Eco53kI GAGCTC 1 cut(s) 469
Eco57I CTGAAG 2 cut(s) 213, 594
Eco88I CYCGRG 1 cut(s) 1063
EcoICRI GAGCTC 1 cut(s) 469
EcoRII CCWGG 2 cut(s) 864, 1123
EcoT14I CCWWGG 3 cut(s) 1098, 1145, 1401
EcoT22I ATGCAT 1 cut(s) 595
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 3 cut(s) 1098, 1145, 1401
Esp3I CGTCTC 1 cut(s) 607
FaqI GGGAC 1 cut(s) 366
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 2 cut(s) 1290, 1293
FokI GGATG 2 cut(s) 117, 278
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 2 cut(s) 1290, 1293
FspBI CTAG 5 cut(s) 29, 174, 356, 1146, 1251
GlaI GCGC 1 cut(s) 1343
GluI GCNGC 2 cut(s) 1290, 1293
GsaI CCCAGC 4 cut(s) 201, 399, 419, 567
GsuI CTGGAG 2 cut(s) 295, 1058
HaeIII GGCC 5 cut(s) 456, 895, 1112, 1231, 1244
HapII CCGG 4 cut(s) 966, 1228, 1245, 1280
HgaI GACGC 2 cut(s) 13, 1036
HhaI GCGC 1 cut(s) 1344
Hin1I GRCGYC 1 cut(s) 1047
Hin6I GCGC 1 cut(s) 1342
HinP1I GCGC 1 cut(s) 1342
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HindIII AAGCTT 1 cut(s) 922
HinfI GANTC 8 cut(s) 57, 308, 411, 572, 847, 938, 1203, 1321
HpaII CCGG 4 cut(s) 966, 1228, 1245, 1280
HphI GGTGA 2 cut(s) 226, 942
Hpy166II GTNNAC 5 cut(s) 331, 361, 751, 979, 1024
Hpy188I TCNGA 9 cut(s) 114, 231, 377, 493, 577, 587, 613, 818, 943
Hpy188III TCNNGA 9 cut(s) 86, 174, 802, 854, 904, 1065, 1075, 1280, 1382
Hpy8I GTNNAC 5 cut(s) 331, 361, 751, 979, 1024
Hpy99I CGWCG 1 cut(s) 1052
HpyAV CCTTC 2 cut(s) 125, 1102
HpyCH4III ACNGT 2 cut(s) 497, 1236
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 6 cut(s) 38, 166, 361, 439, 593, 1289
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 1109, 1228
HpySE526I ACGT 1 cut(s) 600
Hsp92I GRCGYC 1 cut(s) 1047
HspAI GCGC 1 cut(s) 1342
Kpn2I TCCGGA 1 cut(s) 1279
Kzo9I GATC 8 cut(s) 15, 82, 109, 170, 917, 1001, 1067, 1395
Lsp1109I GCAGC 2 cut(s) 1279, 1301
LweI GCATC 3 cut(s) 154, 1181, 1387
MaeI CTAG 5 cut(s) 29, 174, 356, 1146, 1251
MaeII ACGT 1 cut(s) 600
MalI GATC 8 cut(s) 17, 84, 111, 172, 919, 1003, 1069, 1397
MboI GATC 8 cut(s) 15, 82, 109, 170, 917, 1001, 1067, 1395
MboII GAAGA 6 cut(s) 101, 131, 134, 517, 600, 847
MflI RGATCY 1 cut(s) 1395
MhlI GDGCHC 4 cut(s) 138, 363, 471, 861
MluCI AATT 5 cut(s) 386, 520, 651, 667, 698
MlyI GAGTC 4 cut(s) 66, 302, 566, 947
MmeI TCCRAC 1 cut(s) 209
Mph1103I ATGCAT 1 cut(s) 595
MroI TCCGGA 1 cut(s) 1279
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 4 cut(s) 389, 597, 993, 1332
MslI CAYNNNNRTG 4 cut(s) 65, 252, 366, 787
MspA1I CMGCKG 2 cut(s) 419, 1292
MspI CCGG 4 cut(s) 966, 1228, 1245, 1280
MspR9I CCNGG 2 cut(s) 866, 1125
MvaI CCWGG 2 cut(s) 866, 1125
MvnI CGCG 1 cut(s) 1344
MwoI GCNNNNNNNGC 3 cut(s) 142, 1109, 1228
NcoI CCATGG 1 cut(s) 1401
NdeII GATC 8 cut(s) 15, 82, 109, 170, 917, 1001, 1067, 1395
NlaIV GGNNCC 6 cut(s) 158, 648, 1014, 1096, 1143, 1397
NmeAIII GCCGAG 1 cut(s) 1193
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 1 cut(s) 1022
PagI TCATGA 2 cut(s) 853, 1381
PciI ACATGT 1 cut(s) 1018
PcsI WCGNNNNNNNCGW 2 cut(s) 822, 1220
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 4 cut(s) 411, 847, 1203, 1321
PkrI GCNGC 2 cut(s) 1291, 1294
PleI GAGTC 4 cut(s) 65, 302, 566, 946
PpsI GAGTC 4 cut(s) 65, 302, 566, 946
PscI ACATGT 1 cut(s) 1018
Psp124BI GAGCTC 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 864, 1123
PspFI CCCAGC 4 cut(s) 197, 395, 415, 563
PspGI CCWGG 2 cut(s) 864, 1123
PspN4I GGNNCC 6 cut(s) 158, 648, 1014, 1096, 1143, 1397
PspPI GGNCC 4 cut(s) 319, 454, 647, 1277
PsuI RGATCY 1 cut(s) 1395
PvuII CAGCTG 2 cut(s) 419, 1292
RsaI GTAC 4 cut(s) 302, 407, 511, 1151
RsaNI GTAC 4 cut(s) 301, 406, 510, 1150
RseI CAYNNNNRTG 4 cut(s) 65, 252, 366, 787
SacI GAGCTC 1 cut(s) 471
SalI GTCGAC 1 cut(s) 749
SaqAI TTAA 4 cut(s) 389, 597, 993, 1332
SatI GCNGC 2 cut(s) 1290, 1293
Sau3AI GATC 8 cut(s) 15, 82, 109, 170, 917, 1001, 1067, 1395
Sau96I GGNCC 4 cut(s) 319, 454, 647, 1277
SchI GAGTC 4 cut(s) 66, 302, 566, 947
ScrFI CCNGG 2 cut(s) 866, 1125
SduI GDGCHC 4 cut(s) 138, 363, 471, 861
SfaNI GCATC 3 cut(s) 154, 1181, 1387
SinI GGWCC 3 cut(s) 319, 647, 1277
SmiMI CAYNNNNRTG 4 cut(s) 65, 252, 366, 787
SmlI CTYRAG 2 cut(s) 464, 1190
SmoI CTYRAG 2 cut(s) 464, 1190
SpeI ACTAGT 1 cut(s) 355
Sse9I AATT 5 cut(s) 386, 520, 651, 667, 698
SsiI CCGC 3 cut(s) 953, 1370, 1406
SspMI CTAG 5 cut(s) 29, 174, 356, 1146, 1251
SstI GAGCTC 1 cut(s) 471
StyD4I CCNGG 2 cut(s) 864, 1123
StyI CCWWGG 3 cut(s) 1098, 1145, 1401
TaaI ACNGT 2 cut(s) 497, 1236
TaiI ACGT 1 cut(s) 603
TaqI TCGA 4 cut(s) 81, 750, 825, 1004
TasI AATT 5 cut(s) 386, 520, 651, 667, 698
TatI WGTACW 1 cut(s) 405
TfiI GAWTC 4 cut(s) 411, 847, 1203, 1321
Tru1I TTAA 4 cut(s) 389, 597, 993, 1332
Tru9I TTAA 4 cut(s) 389, 597, 993, 1332
TseI GCWGC 2 cut(s) 1289, 1292
TspDTI ATGAA 6 cut(s) 280, 287, 384, 517, 725, 1121
VneI GTGCAC 1 cut(s) 359
VpaK11BI GGWCC 3 cut(s) 319, 647, 1277
XapI RAATTY 2 cut(s) 520, 698
XbaI TCTAGA 1 cut(s) 173
XceI RCATGY 1 cut(s) 1022
XmaJI CCTAGG 1 cut(s) 1145
XmiI GTMKAC 1 cut(s) 750
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 5 cut(s) 29, 174, 356, 1146, 1251
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.