Rroxscaffold_1G00020220

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001754
Physical Location & Seq
Forward (+)
24559056 .. 24561206
2151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00020220.1

Sequence Viewer

Length: 1410 bp
ATGGGAAACAGAGTGATGGTGAGCCTCCTGGTTCTTGTGCTTGTGCTTTCCTATGGAGTTATGGCAATGGCTATGAGGTTTAATCATGAAGATGAAGAGTGGGGAAGAGATCAGAGAGAAGAAGAGTGGGAAGGGCACCAAGGGCATCGCCATCTTTTCCTTTTGCAACAATCAAGGCTCCTGGTGAAAACTGAAGCTGGGCAAATGAGGGTGGTGAGAAGTGTAGGTCAGAGAATGGTAGATAGGCATATAAATGTTGGCTATCTCACCTTGGAACCTAACTCCCTCTTCATCCCCCAGTACCTCGACTCCAGCTTGATCCTCTTTGTCCAAAGAGGGCAAGCAAATGTTGGACTGGTACACCGACAGGAATTGGGGGAGAGAAAATTGAAGGCTGGGGATGTGTACAGAATTCCAGCAGGTTCCACATTTTACTTGCAGAATGTCAGGGAGAACAGGAGACTTCAGATCATTCTCAGCATCGACACATCTGACAGTTTGAGAATGGGTACTTTGGAGAATTTCTTCATAGGTGGAGGAACCTACCCAAAATCTGTACTTTCTGGGTTCGATTCTGAGATACTCACAAATGCATTTAACGTCTCATCTTCAGAAATAAGGCAGGTTTTGACAAGGCACCAAGAGGGTCCAATTATATATCTAAATAATTCCCAATCATCATCAAACCTTTGGACCAAATTCGTAACCATGAAAGAGCAGGACAGACTACAAGAATTGAAGAAAATGGTGGACTTCCCAGAAGAACCAGAGCAAACCCTAACTTGTTCATGGAGGAAACTGTTGAACTCCGTGTTCGGAACACTTGAAAATGAGGAGAGGAAGCAACATCATCATGAGCACTCCCAGAAATCCTACAACCTCTATGATAGAAAGCCAGATTTTAAGAACCACCATGGATGGACCCTCTCAGTTGATGAGTCTGATTACTCTCCCCTAAAGGACTCCGGCATTGGCGTTTACCTTGTTAGTCTCAACGCAGGGTCAATGTTGGCACCACATGTGAACCCAAGAGCAACAGAATACGGCGTCGTTCTGAGAGGCTCAGGATCACTACAGATTGTATTTCCAAATGGTACCTTGGCAATGAAGGCGAAACTAAAGCCAGGGAGAGTGTTTTGGGTGCCAAGGTATTTCCCATTTGTCCAAGTGGCATCTAACAACGAGCAACTTGAGATTTTTGGGTTCACTACCTCAGCACAGCCGAACCGGCCACAGTTTTTGGCTGGCTCTAGCTCGGTGCTGCAAGCTCTAAGAGGTCAAGAACTTGCAGCTGCTTTTGGTGTGAGTGAGGGAAGGTTGAATCATTTTGTTAATGCTCAACGCGAAGCTGTCATTTTGCCATACAATGCAGAGAGGAATCAAAAGGCAGATGGGATGGCAGACCGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

469

Amino Acids

53.28

Weight (kDa)

8.44

Isoelectric Point (pI)

40.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 55 - 206 2.3e-11 Cupin
Cupin_1 PF00190 292 - 437 7.3e-33 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 410, 613
Acc65I GGTACC 1 cut(s) 1094
AccB1I GGYRCC 5 cut(s) 135, 636, 1012, 1094, 1141
AccII CGCG 1 cut(s) 1344
AclWI GGATC 2 cut(s) 313, 1075
AcoI YGGCCR 1 cut(s) 1229
AcsI RAATTY 3 cut(s) 411, 520, 698
AcuI CTGAAG 3 cut(s) 213, 449, 594
AcyI GRCGYC 1 cut(s) 1047
AfaI GTAC 6 cut(s) 302, 360, 407, 511, 558, 1096
AflIII ACRYGT 1 cut(s) 1018
AgeI ACCGGT 1 cut(s) 1404
AgsI TTSAA 5 cut(s) 391, 739, 805, 827, 1321
AjnI CCWGG 3 cut(s) 27, 180, 1123
AluBI AGCT 6 cut(s) 197, 315, 1254, 1268, 1292, 1349
AluI AGCT 6 cut(s) 197, 315, 1254, 1268, 1292, 1349
Alw21I GWGCWC 1 cut(s) 861
Alw26I GTCTC 3 cut(s) 454, 607, 995
AlwI GGATC 2 cut(s) 313, 1075
AoxI GGCC 1 cut(s) 1229
ApeKI GCWGC 3 cut(s) 1261, 1289, 1292
ApoI RAATTY 3 cut(s) 411, 520, 698
AsiGI ACCGGT 1 cut(s) 1404
Asp700I GAANNNNTTC 1 cut(s) 524
Asp718I GGTACC 1 cut(s) 1094
AspS9I GGNCC 3 cut(s) 647, 693, 921
AsuHPI GGTGA 4 cut(s) 31, 196, 226, 259
AvaII GGWCC 3 cut(s) 647, 693, 921
BaeGI GKGCMC 1 cut(s) 138
BanI GGYRCC 5 cut(s) 135, 636, 1012, 1094, 1141
Bbv12I GWGCWC 1 cut(s) 861
BbvCI CCTCAGC 1 cut(s) 1213
BbvI GCAGC 3 cut(s) 1248, 1279, 1301
BccI CCATC 5 cut(s) 10, 159, 912, 1385, 1390
BceAI ACGGC 1 cut(s) 1060
BciT130I CCWGG 3 cut(s) 29, 182, 1125
BcoDI GTCTC 3 cut(s) 454, 607, 995
BfaI CTAG 1 cut(s) 1251
BfmI CTRYAG 1 cut(s) 1073
BfuAI ACCTGC 2 cut(s) 410, 613
BglI GCCNNNNNGGC 1 cut(s) 1228
BisI GCNGC 3 cut(s) 1262, 1290, 1293
BlsI GCNGC 3 cut(s) 1263, 1291, 1294
Bme1390I CCNGG 3 cut(s) 29, 182, 1125
Bme18I GGWCC 3 cut(s) 647, 693, 921
BmgT120I GGNCC 3 cut(s) 647, 693, 921
BmrFI CCNGG 3 cut(s) 29, 182, 1125
BmrI ACTGGG 1 cut(s) 292
BmsI GCATC 3 cut(s) 154, 489, 1181
BmuI ACTGGG 1 cut(s) 292
BpmI CTGGAG 1 cut(s) 295
Bpu10I CCTNAGC 2 cut(s) 1063, 1213
BpuEI CTTGAG 1 cut(s) 1211
BsaHI GRCGYC 1 cut(s) 1047
BsaJI CCNNGG 6 cut(s) 139, 270, 913, 1098, 1124, 1145
BsaWI WCCGGW 1 cut(s) 1404
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bse118I RCCGGY 2 cut(s) 1227, 1404
Bse1I ACTGG 2 cut(s) 298, 360
Bse3DI GCAATG 2 cut(s) 72, 1110
BseBI CCWGG 3 cut(s) 29, 182, 1125
BseDI CCNNGG 6 cut(s) 139, 270, 913, 1098, 1124, 1145
BseGI GGATG 4 cut(s) 291, 406, 923, 1401
BseMI GCAATG 2 cut(s) 72, 1110
BseMII CTCAG 6 cut(s) 490, 567, 942, 1046, 1077, 1227
BseNI ACTGG 2 cut(s) 298, 360
BseRI GAGGAG 1 cut(s) 848
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 3 cut(s) 1248, 1279, 1301
BseYI CCCAGC 2 cut(s) 197, 395
Bsh1236I CGCG 1 cut(s) 1344
BshFI GGCC 1 cut(s) 1231
BshNI GGYRCC 5 cut(s) 135, 636, 1012, 1094, 1141
BshTI ACCGGT 1 cut(s) 1404
BsiHKAI GWGCWC 1 cut(s) 861
BsiSI CCGG 3 cut(s) 966, 1228, 1405
BsmAI GTCTC 3 cut(s) 454, 607, 995
BsmBI CGTCTC 1 cut(s) 607
BsnI GGCC 1 cut(s) 1231
Bsp1286I GDGCHC 2 cut(s) 138, 861
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 4 cut(s) 109, 318, 468, 1067
Bsp19I CCATGG 1 cut(s) 913
BspANI GGCC 1 cut(s) 1231
BspCNI CTCAG 6 cut(s) 489, 568, 941, 1047, 1076, 1226
BspFNI CGCG 1 cut(s) 1344
BspHI TCATGA 2 cut(s) 85, 853
BspMI ACCTGC 2 cut(s) 410, 613
BspPI GGATC 2 cut(s) 313, 1075
BspT107I GGYRCC 5 cut(s) 135, 636, 1012, 1094, 1141
BsrDI GCAATG 2 cut(s) 72, 1110
BsrFI RCCGGY 2 cut(s) 1227, 1404
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 2 cut(s) 298, 360
BssAI RCCGGY 2 cut(s) 1227, 1404
BssECI CCNNGG 6 cut(s) 139, 270, 913, 1098, 1124, 1145
BssMI GATC 4 cut(s) 109, 318, 468, 1067
BssNI GRCGYC 1 cut(s) 1047
BssT1I CCWWGG 5 cut(s) 139, 270, 913, 1098, 1145
Bst2UI CCWGG 3 cut(s) 29, 182, 1125
Bst4CI ACNGT 3 cut(s) 497, 801, 1236
Bst6I CTCTTC 4 cut(s) 90, 100, 117, 293
BstACI GRCGYC 1 cut(s) 1047
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 3 cut(s) 342, 1246, 1266
BstDEI CTNAG 7 cut(s) 476, 576, 928, 1055, 1063, 1213, 1271
BstDSI CCRYGG 1 cut(s) 913
BstF5I GGATG 4 cut(s) 291, 406, 923, 1401
BstFNI CGCG 1 cut(s) 1344
BstKTI GATC 4 cut(s) 112, 321, 471, 1070
BstMAI GTCTC 3 cut(s) 454, 607, 995
BstMBI GATC 4 cut(s) 109, 318, 468, 1067
BstMWI GCNNNNNNNGC 3 cut(s) 142, 1109, 1228
BstNI CCWGG 3 cut(s) 29, 182, 1125
BstNSI RCATGY 1 cut(s) 1022
BstSCI CCNGG 3 cut(s) 27, 180, 1123
BstSFI CTRYAG 1 cut(s) 1073
BstSLI GKGCMC 1 cut(s) 138
BstUI CGCG 1 cut(s) 1344
BstV1I GCAGC 3 cut(s) 1248, 1279, 1301
BsuRI GGCC 1 cut(s) 1231
BtgI CCRYGG 1 cut(s) 913
BtgZI GCGATG 1 cut(s) 131
BtsCI GGATG 4 cut(s) 291, 406, 923, 1401
BveI ACCTGC 2 cut(s) 410, 613
Cac8I GCNNGC 3 cut(s) 342, 1246, 1266
CciI TCATGA 2 cut(s) 85, 853
Cfr10I RCCGGY 2 cut(s) 1227, 1404
Cfr13I GGNCC 3 cut(s) 647, 693, 921
CseI GACGC 1 cut(s) 1036
Csp6I GTAC 6 cut(s) 301, 359, 406, 510, 557, 1095
CspAI ACCGGT 1 cut(s) 1404
CspCI CAANNNNNGTGG 2 cut(s) 1221, 1256
CviAII CATG 6 cut(s) 86, 709, 789, 854, 914, 1019
CviQI GTAC 6 cut(s) 301, 359, 406, 510, 557, 1095
DdeI CTNAG 7 cut(s) 476, 576, 928, 1055, 1063, 1213, 1271
DpnI GATC 4 cut(s) 111, 320, 470, 1069
DpnII GATC 4 cut(s) 109, 318, 468, 1067
EaeI YGGCCR 1 cut(s) 1229
Eam1104I CTCTTC 4 cut(s) 90, 100, 117, 293
EarI CTCTTC 4 cut(s) 90, 100, 117, 293
Eco130I CCWWGG 5 cut(s) 139, 270, 913, 1098, 1145
Eco47I GGWCC 3 cut(s) 647, 693, 921
Eco57I CTGAAG 3 cut(s) 213, 449, 594
EcoRI GAATTC 1 cut(s) 411
EcoRII CCWGG 3 cut(s) 27, 180, 1123
EcoT14I CCWWGG 5 cut(s) 139, 270, 913, 1098, 1145
EcoT22I ATGCAT 1 cut(s) 595
ErhI CCWWGG 5 cut(s) 139, 270, 913, 1098, 1145
Esp3I CGTCTC 1 cut(s) 607
FaeI CATG 6 cut(s) 89, 712, 792, 857, 917, 1022
FatI CATG 6 cut(s) 85, 708, 788, 853, 913, 1018
Fnu4HI GCNGC 3 cut(s) 1262, 1290, 1293
FokI GGATG 3 cut(s) 278, 413, 930
Fsp4HI GCNGC 3 cut(s) 1262, 1290, 1293
FspBI CTAG 1 cut(s) 1251
GluI GCNGC 3 cut(s) 1262, 1290, 1293
GsaI CCCAGC 2 cut(s) 201, 399
GsuI CTGGAG 1 cut(s) 295
HaeIII GGCC 1 cut(s) 1231
HapII CCGG 3 cut(s) 966, 1228, 1405
HgaI GACGC 1 cut(s) 1036
Hin1I GRCGYC 1 cut(s) 1047
Hin1II CATG 6 cut(s) 89, 712, 792, 857, 917, 1022
HinfI GANTC 6 cut(s) 308, 572, 938, 962, 1321, 1378
HpaII CCGG 3 cut(s) 966, 1228, 1405
HphI GGTGA 4 cut(s) 31, 196, 226, 259
Hpy166II GTNNAC 6 cut(s) 361, 406, 751, 979, 1024, 1206
Hpy188I TCNGA 9 cut(s) 114, 231, 468, 493, 577, 613, 818, 943, 1056
Hpy188III TCNNGA 4 cut(s) 86, 854, 1065, 1280
Hpy8I GTNNAC 6 cut(s) 361, 406, 751, 979, 1024, 1206
Hpy99I CGWCG 1 cut(s) 1052
HpyAV CCTTC 4 cut(s) 125, 385, 1102, 1308
HpyCH4III ACNGT 3 cut(s) 497, 801, 1236
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 6 cut(s) 166, 439, 593, 1264, 1289, 1370
HpyF10VI GCNNNNNNNGC 3 cut(s) 142, 1109, 1228
HpyF3I CTNAG 7 cut(s) 476, 576, 928, 1055, 1063, 1213, 1271
HpySE526I ACGT 1 cut(s) 600
Hsp92I GRCGYC 1 cut(s) 1047
Hsp92II CATG 6 cut(s) 89, 712, 792, 857, 917, 1022
KpnI GGTACC 1 cut(s) 1098
Kzo9I GATC 4 cut(s) 109, 318, 468, 1067
LmnI GCTCC 1 cut(s) 183
Lsp1109I GCAGC 3 cut(s) 1248, 1279, 1301
LweI GCATC 3 cut(s) 154, 489, 1181
MaeI CTAG 1 cut(s) 1251
MaeII ACGT 1 cut(s) 600
MaeIII GTNAC 1 cut(s) 703
MalI GATC 4 cut(s) 111, 320, 470, 1069
MboI GATC 4 cut(s) 109, 318, 468, 1067
MhlI GDGCHC 2 cut(s) 138, 861
MluCI AATT 8 cut(s) 371, 386, 411, 520, 651, 667, 698, 734
MlyI GAGTC 3 cut(s) 302, 947, 956
MmeI TCCRAC 1 cut(s) 331
Mph1103I ATGCAT 1 cut(s) 595
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 4 cut(s) 81, 597, 903, 1332
MslI CAYNNNNRTG 3 cut(s) 90, 252, 852
MspA1I CMGCKG 1 cut(s) 1292
MspI CCGG 3 cut(s) 966, 1228, 1405
MspR9I CCNGG 3 cut(s) 29, 182, 1125
MvaI CCWGG 3 cut(s) 29, 182, 1125
MvnI CGCG 1 cut(s) 1344
MwoI GCNNNNNNNGC 3 cut(s) 142, 1109, 1228
NcoI CCATGG 1 cut(s) 913
NdeII GATC 4 cut(s) 109, 318, 468, 1067
NlaIII CATG 6 cut(s) 89, 712, 792, 857, 917, 1022
NsiI ATGCAT 1 cut(s) 595
NspI RCATGY 1 cut(s) 1022
PagI TCATGA 2 cut(s) 85, 853
PciI ACATGT 1 cut(s) 1018
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 3 cut(s) 572, 1321, 1378
PinAI ACCGGT 1 cut(s) 1404
PkrI GCNGC 3 cut(s) 1263, 1291, 1294
PleI GAGTC 3 cut(s) 302, 946, 956
PpsI GAGTC 3 cut(s) 302, 946, 956
PscI ACATGT 1 cut(s) 1018
Psp6I CCWGG 3 cut(s) 27, 180, 1123
PspFI CCCAGC 2 cut(s) 197, 395
PspGI CCWGG 3 cut(s) 27, 180, 1123
PspPI GGNCC 3 cut(s) 647, 693, 921
PvuII CAGCTG 1 cut(s) 1292
RsaI GTAC 6 cut(s) 302, 360, 407, 511, 558, 1096
RsaNI GTAC 6 cut(s) 301, 359, 406, 510, 557, 1095
RseI CAYNNNNRTG 3 cut(s) 90, 252, 852
SaqAI TTAA 4 cut(s) 81, 597, 903, 1332
SatI GCNGC 3 cut(s) 1262, 1290, 1293
Sau3AI GATC 4 cut(s) 109, 318, 468, 1067
Sau96I GGNCC 3 cut(s) 647, 693, 921
SchI GAGTC 3 cut(s) 302, 947, 956
ScrFI CCNGG 3 cut(s) 29, 182, 1125
SduI GDGCHC 2 cut(s) 138, 861
SfaNI GCATC 3 cut(s) 154, 489, 1181
SfcI CTRYAG 1 cut(s) 1073
SinI GGWCC 3 cut(s) 647, 693, 921
SmiMI CAYNNNNRTG 3 cut(s) 90, 252, 852
SmlI CTYRAG 1 cut(s) 1190
SmoI CTYRAG 1 cut(s) 1190
Sse9I AATT 8 cut(s) 371, 386, 411, 520, 651, 667, 698, 734
SspMI CTAG 1 cut(s) 1251
StyD4I CCNGG 3 cut(s) 27, 180, 1123
StyI CCWWGG 5 cut(s) 139, 270, 913, 1098, 1145
TaaI ACNGT 3 cut(s) 497, 801, 1236
TaiI ACGT 1 cut(s) 603
TaqI TCGA 3 cut(s) 306, 483, 570
TasI AATT 8 cut(s) 371, 386, 411, 520, 651, 667, 698, 734
TatI WGTACW 2 cut(s) 405, 556
TfiI GAWTC 3 cut(s) 572, 1321, 1378
Tru1I TTAA 4 cut(s) 81, 597, 903, 1332
Tru9I TTAA 4 cut(s) 81, 597, 903, 1332
TseI GCWGC 3 cut(s) 1261, 1289, 1292
TspDTI ATGAA 7 cut(s) 102, 108, 280, 517, 725, 777, 1121
TspGWI ACGGA 1 cut(s) 799
VpaK11BI GGWCC 3 cut(s) 647, 693, 921
XapI RAATTY 3 cut(s) 411, 520, 698
XceI RCATGY 1 cut(s) 1022
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 1 cut(s) 1251
Zsp2I ATGCAT 1 cut(s) 595
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.