Rroxscaffold_1G00020280

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0001754
Physical Location & Seq
Forward (+)
24632872 .. 24634799
1928 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00020280.1

Sequence Viewer

Length: 1416 bp
ATGGGAAAGAGAGTGATGGTGAGCGTCCTGGTTCTTGCAATTGTGATGTGCTTTGGAGTCATGGCAATGGCTATGAGCTTCGATCACGAAGACGAGGACTGGCGAAGGGATCATAGAGAAGAAGAGTGGGAAGGGCACAGAAGGCATCGCCATTGGTTCCTTTTGCAACGATCTAGACTACTGCTGAGAACTGAAGCTGGGGAAATGAGGGTGGTGAGAAGTGTAGGTCAGAGAATGGTTGATAGACATATAAATGTTGGGTTTCTCTCCATGGAACCCAACTCTCTTTTCATCCCTCAGTACCTTGACTCCAGCGTGGTCCTCTTTGTCCACACAGGGCAAGCAAATGTGGGATTGGTACGCCAACATGAACTCGGAGAGAGGCAATTGAAGGCTGGGGATTTGTACAGAATCCCAGCTGGTTCAACATTTTACTTGCAAAATGTTGGGGAGGGCCAGAGACTTGAGCTCATTCTCAGCATTGACACATCTGATAGTTTAAGAATGGGTACTTTGGAGAATTTCTTCATTGGTGGAGGAACCTACCCAAAATCCGTCCTTGCTGGGTTCGATTCTCAAGTACTCACAAGTGCATTTAACGTCTCATCTTCAGAACTAAGGCACATTTTGACAAGGCAACAAGAGGGTCCAATTGTATCTCTGAATAATTCTCAATCATCATCAAACCTTTGGACAAAATTCCTAAGCATGAAAGAGCAGGACAGACTACAAGAACTAAAGAAAATGGTCGACTTCCCACAAGAACAAGAGCAAACCCAAACATGGTCATGGAGGAAACTCTTGACCTCTATGTTCGGAATAATCGAAAACGAGAAGAGGAAGCAAAATCATCATGAGCACTCCCAGAAGGCTTACAACCTCTATGCGAGGAGGCCGGATTTCAAGAACAACTATGGATCGAGCTTGTCAGTTGATGAGTCTGATTACTCACCGCTACAAAACTCCGGCATTGGCGTTTACCTTGTCAATCTTAACGCAGGATCGATGTTGGCACCACATGTGAACCCAGCTGCAACAGAATACGGCGTCGTTCTAAGAGGCTCAGGATCACTCCAGATTGTGTTTCCAAACGGAACCTTGGCAATGAAGGCGAAACTAAAACCAGGGAGAGTGTTCTGGGTGCCGAGGTACTTCCCATTTGTCCAAGTGGCATCTAACAACGAGCAACTTCAGATTTTCGGGTTCACTACCTCAAAGCAGCCGAACCACCCACAATTTCTGGCCGGTTCTAGCTCGGTGCTTGAAGCTCTAAGAGGTCCGGAACTTGCAGCTGCTTTTGGTGTGAGTGAGGGAAGGTTGAATCGTTTTGTTAATGCTCAGCGCGAAGCTGTAATCTTACCTTACAATGCGGAGAGGCATCATGAGACACATGAGATCCCCTTGGCGGACTGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

471

Amino Acids

53.52

Weight (kDa)

7.92

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Cupin_1 PF00190 80 - 205 1.1e-09 Cupin
Cupin_1 PF00190 292 - 437 1e-30 Cupin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 1012, 1141
AccI GTMKAC 1 cut(s) 750
AccII CGCG 1 cut(s) 1344
AccIII TCCGGA 1 cut(s) 1279
AciI CCGC 3 cut(s) 953, 1370, 1406
AclWI GGATC 5 cut(s) 117, 925, 1009, 1075, 1390
AcoI YGGCCR 1 cut(s) 1242
AcsI RAATTY 2 cut(s) 520, 698
AcuI CTGAAG 3 cut(s) 213, 594, 1175
AcyI GRCGYC 1 cut(s) 1047
AfaI GTAC 6 cut(s) 302, 360, 407, 511, 582, 1151
AfiI CCNNNNNNNGG 2 cut(s) 783, 1405
AflIII ACRYGT 1 cut(s) 1018
AgsI TTSAA 5 cut(s) 391, 426, 904, 1265, 1321
AjnI CCWGG 2 cut(s) 27, 1123
AjuI GAANNNNNNNTTGG 2 cut(s) 272, 304
Alw21I GWGCWC 2 cut(s) 471, 861
Alw26I GTCTC 3 cut(s) 454, 607, 1379
AlwI GGATC 5 cut(s) 117, 925, 1009, 1075, 1390
Aor13HI TCCGGA 1 cut(s) 1279
AoxI GGCC 3 cut(s) 454, 893, 1242
ApeKI GCWGC 4 cut(s) 1031, 1219, 1289, 1292
ApoI RAATTY 2 cut(s) 520, 698
Asp700I GAANNNNTTC 1 cut(s) 524
AspLEI GCGC 1 cut(s) 1344
AspS9I GGNCC 4 cut(s) 319, 454, 647, 1277
AsuHPI GGTGA 3 cut(s) 31, 226, 942
AvaII GGWCC 3 cut(s) 319, 647, 1277
BaeGI GKGCMC 1 cut(s) 138
BanI GGYRCC 2 cut(s) 1012, 1141
BanII GRGCYC 1 cut(s) 471
BbsI GAAGAC 1 cut(s) 96
Bbv12I GWGCWC 2 cut(s) 471, 861
BbvI GCAGC 4 cut(s) 1018, 1231, 1279, 1301
BccI CCATC 1 cut(s) 10
BceAI ACGGC 1 cut(s) 1060
BciT130I CCWGG 2 cut(s) 29, 1125
BcoDI GTCTC 3 cut(s) 454, 607, 1379
BfaI CTAG 2 cut(s) 174, 1251
BisI GCNGC 4 cut(s) 1032, 1220, 1290, 1293
BlpI GCTNAGC 1 cut(s) 1338
BlsI GCNGC 4 cut(s) 1033, 1221, 1291, 1294
BmcAI AGTACT 1 cut(s) 582
Bme1390I CCNGG 2 cut(s) 29, 1125
Bme18I GGWCC 3 cut(s) 319, 647, 1277
BmgT120I GGNCC 4 cut(s) 319, 454, 647, 1277
BmiI GGNNCC 7 cut(s) 158, 276, 541, 648, 1014, 1096, 1143
BmrFI CCNGG 2 cut(s) 29, 1125
BmsI GCATC 3 cut(s) 154, 1181, 1387
BpiI GAAGAC 1 cut(s) 96
BpmI CTGGAG 2 cut(s) 295, 1058
Bpu10I CCTNAGC 2 cut(s) 704, 1063
Bpu1102I GCTNAGC 1 cut(s) 1338
BpuEI CTTGAG 2 cut(s) 485, 561
Bsa29I ATCGAT 1 cut(s) 1004
BsaHI GRCGYC 1 cut(s) 1047
BsaJI CCNNGG 5 cut(s) 270, 1098, 1124, 1145, 1401
BsaWI WCCGGW 1 cut(s) 1279
BsaXI ACNNNNNCTCC 2 cut(s) 293, 323
Bsc4I CCNNNNNNNGG 2 cut(s) 783, 1405
Bse118I RCCGGY 1 cut(s) 1244
Bse1I ACTGG 2 cut(s) 104, 1415
Bse3DI GCAATG 2 cut(s) 72, 1110
BseAI TCCGGA 1 cut(s) 1279
BseBI CCWGG 2 cut(s) 29, 1125
BseCI ATCGAT 1 cut(s) 1004
BseDI CCNNGG 5 cut(s) 270, 1098, 1124, 1145, 1401
BseGI GGATG 1 cut(s) 291
BseLI CCNNNNNNNGG 2 cut(s) 783, 1405
BseMI GCAATG 2 cut(s) 72, 1110
BseMII CTCAG 5 cut(s) 176, 311, 490, 1077, 1352
BseNI ACTGG 2 cut(s) 104, 1415
BseRI GAGGAG 1 cut(s) 904
BseSI GKGCMC 1 cut(s) 138
BseXI GCAGC 4 cut(s) 1018, 1231, 1279, 1301
BseYI CCCAGC 5 cut(s) 197, 395, 415, 563, 1027
Bsh1236I CGCG 1 cut(s) 1344
BshFI GGCC 3 cut(s) 456, 895, 1244
BshNI GGYRCC 2 cut(s) 1012, 1141
BshVI ATCGAT 1 cut(s) 1004
BsiHKAI GWGCWC 2 cut(s) 471, 861
BsiSI CCGG 4 cut(s) 896, 966, 1245, 1280
BslI CCNNNNNNNGG 2 cut(s) 783, 1405
BsmAI GTCTC 3 cut(s) 454, 607, 1379
BsmBI CGTCTC 1 cut(s) 607
BsnI GGCC 3 cut(s) 456, 895, 1244
Bsp1286I GDGCHC 3 cut(s) 138, 471, 861
Bsp13I TCCGGA 1 cut(s) 1279
Bsp1407I TGTACA 1 cut(s) 405
Bsp143I GATC 7 cut(s) 82, 109, 170, 917, 1001, 1067, 1395
Bsp1720I GCTNAGC 1 cut(s) 1338
Bsp19I CCATGG 1 cut(s) 270
BspACI CCGC 3 cut(s) 953, 1370, 1406
BspANI GGCC 3 cut(s) 456, 895, 1244
BspCNI CTCAG 5 cut(s) 177, 310, 489, 1076, 1351
BspDI ATCGAT 1 cut(s) 1004
BspEI TCCGGA 1 cut(s) 1279
BspFNI CGCG 1 cut(s) 1344
BspHI TCATGA 2 cut(s) 853, 1381
BspLI GGNNCC 7 cut(s) 158, 276, 541, 648, 1014, 1096, 1143
BspPI GGATC 5 cut(s) 117, 925, 1009, 1075, 1390
BspT107I GGYRCC 2 cut(s) 1012, 1141
BsrDI GCAATG 2 cut(s) 72, 1110
BsrFI RCCGGY 1 cut(s) 1244
BsrGI TGTACA 1 cut(s) 405
BsrI ACTGG 2 cut(s) 104, 1415
BssAI RCCGGY 1 cut(s) 1244
BssECI CCNNGG 5 cut(s) 270, 1098, 1124, 1145, 1401
BssMI GATC 7 cut(s) 82, 109, 170, 917, 1001, 1067, 1395
BssNI GRCGYC 1 cut(s) 1047
BssT1I CCWWGG 3 cut(s) 270, 1098, 1401
Bst2UI CCWGG 2 cut(s) 29, 1125
Bst6I CTCTTC 2 cut(s) 117, 830
BstACI GRCGYC 1 cut(s) 1047
BstAUI TGTACA 1 cut(s) 405
BstC8I GCNNGC 1 cut(s) 342
BstDEI CTNAG 9 cut(s) 185, 297, 476, 617, 704, 1055, 1063, 1271, 1338
BstDSI CCRYGG 1 cut(s) 270
BstF5I GGATG 1 cut(s) 291
BstFNI CGCG 1 cut(s) 1344
BstHHI GCGC 1 cut(s) 1344
BstKTI GATC 7 cut(s) 85, 112, 173, 920, 1004, 1070, 1398
BstMAI GTCTC 3 cut(s) 454, 607, 1379
BstMBI GATC 7 cut(s) 82, 109, 170, 917, 1001, 1067, 1395
BstMWI GCNNNNNNNGC 2 cut(s) 142, 1109
BstNI CCWGG 2 cut(s) 29, 1125
BstNSI RCATGY 1 cut(s) 1022
BstSCI CCNGG 2 cut(s) 27, 1123
BstSLI GKGCMC 1 cut(s) 138
BstUI CGCG 1 cut(s) 1344
BstV1I GCAGC 4 cut(s) 1018, 1231, 1279, 1301
BstV2I GAAGAC 1 cut(s) 96
BstX2I RGATCY 1 cut(s) 1395
BstYI RGATCY 1 cut(s) 1395
Bsu15I ATCGAT 1 cut(s) 1004
BsuRI GGCC 3 cut(s) 456, 895, 1244
BsuTUI ATCGAT 1 cut(s) 1004
BtgI CCRYGG 1 cut(s) 270
BtgZI GCGATG 1 cut(s) 131
BtsCI GGATG 1 cut(s) 291
Cac8I GCNNGC 1 cut(s) 342
CciI TCATGA 2 cut(s) 853, 1381
CfoI GCGC 1 cut(s) 1344
Cfr10I RCCGGY 1 cut(s) 1244
Cfr13I GGNCC 4 cut(s) 319, 454, 647, 1277
ClaI ATCGAT 1 cut(s) 1004
CseI GACGC 2 cut(s) 13, 1036
Csp6I GTAC 6 cut(s) 301, 359, 406, 510, 581, 1150
CviQI GTAC 6 cut(s) 301, 359, 406, 510, 581, 1150
DdeI CTNAG 9 cut(s) 185, 297, 476, 617, 704, 1055, 1063, 1271, 1338
DpnI GATC 7 cut(s) 84, 111, 172, 919, 1003, 1069, 1397
DpnII GATC 7 cut(s) 82, 109, 170, 917, 1001, 1067, 1395
EaeI YGGCCR 1 cut(s) 1242
Eam1104I CTCTTC 2 cut(s) 117, 830
EarI CTCTTC 2 cut(s) 117, 830
Ecl136II GAGCTC 1 cut(s) 469
Eco130I CCWWGG 3 cut(s) 270, 1098, 1401
Eco24I GRGCYC 1 cut(s) 471
Eco47I GGWCC 3 cut(s) 319, 647, 1277
Eco53kI GAGCTC 1 cut(s) 469
Eco57I CTGAAG 3 cut(s) 213, 594, 1175
EcoICRI GAGCTC 1 cut(s) 469
EcoRII CCWGG 2 cut(s) 27, 1123
EcoT14I CCWWGG 3 cut(s) 270, 1098, 1401
EcoT38I GRGCYC 1 cut(s) 471
ErhI CCWWGG 3 cut(s) 270, 1098, 1401
Esp3I CGTCTC 1 cut(s) 607
FblI GTMKAC 1 cut(s) 750
Fnu4HI GCNGC 4 cut(s) 1032, 1220, 1290, 1293
FokI GGATG 1 cut(s) 278
FriOI GRGCYC 1 cut(s) 471
Fsp4HI GCNGC 4 cut(s) 1032, 1220, 1290, 1293
FspBI CTAG 2 cut(s) 174, 1251
GlaI GCGC 1 cut(s) 1343
GluI GCNGC 4 cut(s) 1032, 1220, 1290, 1293
GsaI CCCAGC 5 cut(s) 201, 399, 419, 567, 1031
GsuI CTGGAG 2 cut(s) 295, 1058
HaeIII GGCC 3 cut(s) 456, 895, 1244
HapII CCGG 4 cut(s) 896, 966, 1245, 1280
HgaI GACGC 2 cut(s) 13, 1036
HhaI GCGC 1 cut(s) 1344
Hin1I GRCGYC 1 cut(s) 1047
Hin6I GCGC 1 cut(s) 1342
HinP1I GCGC 1 cut(s) 1342
HincII GTYRAC 1 cut(s) 751
HindII GTYRAC 1 cut(s) 751
HinfI GANTC 6 cut(s) 57, 308, 411, 572, 938, 1321
HpaII CCGG 4 cut(s) 896, 966, 1245, 1280
HphI GGTGA 3 cut(s) 31, 226, 942
Hpy166II GTNNAC 5 cut(s) 331, 751, 979, 1024, 1206
Hpy188I TCNGA 8 cut(s) 231, 377, 493, 613, 663, 818, 943, 1194
Hpy188III TCNNGA 9 cut(s) 86, 174, 802, 854, 904, 1065, 1075, 1280, 1382
Hpy8I GTNNAC 5 cut(s) 331, 751, 979, 1024, 1206
Hpy99I CGWCG 1 cut(s) 1052
HpyAV CCTTC 7 cut(s) 99, 125, 135, 385, 862, 1102, 1308
HpyCH4IV ACGT 1 cut(s) 600
HpyCH4V TGCA 6 cut(s) 38, 166, 439, 593, 1034, 1289
HpyF10VI GCNNNNNNNGC 2 cut(s) 142, 1109
HpyF3I CTNAG 9 cut(s) 185, 297, 476, 617, 704, 1055, 1063, 1271, 1338
HpySE526I ACGT 1 cut(s) 600
Hsp92I GRCGYC 1 cut(s) 1047
HspAI GCGC 1 cut(s) 1342
Kpn2I TCCGGA 1 cut(s) 1279
Kzo9I GATC 7 cut(s) 82, 109, 170, 917, 1001, 1067, 1395
Lsp1109I GCAGC 4 cut(s) 1018, 1231, 1279, 1301
LweI GCATC 3 cut(s) 154, 1181, 1387
MaeI CTAG 2 cut(s) 174, 1251
MaeII ACGT 1 cut(s) 600
MalI GATC 7 cut(s) 84, 111, 172, 919, 1003, 1069, 1397
MboI GATC 7 cut(s) 82, 109, 170, 917, 1001, 1067, 1395
MboII GAAGA 6 cut(s) 101, 131, 134, 517, 600, 847
MfeI CAATTG 3 cut(s) 39, 386, 651
MflI RGATCY 1 cut(s) 1395
MhlI GDGCHC 3 cut(s) 138, 471, 861
MluCI AATT 7 cut(s) 39, 386, 520, 651, 667, 698, 1235
MlyI GAGTC 3 cut(s) 66, 302, 947
MroI TCCGGA 1 cut(s) 1279
MroXI GAANNNNTTC 1 cut(s) 524
MseI TTAA 4 cut(s) 500, 597, 993, 1332
MslI CAYNNNNRTG 3 cut(s) 65, 252, 787
MspA1I CMGCKG 3 cut(s) 419, 1031, 1292
MspI CCGG 4 cut(s) 896, 966, 1245, 1280
MspR9I CCNGG 2 cut(s) 29, 1125
MunI CAATTG 3 cut(s) 39, 386, 651
MvaI CCWGG 2 cut(s) 29, 1125
MvnI CGCG 1 cut(s) 1344
MwoI GCNNNNNNNGC 2 cut(s) 142, 1109
NcoI CCATGG 1 cut(s) 270
NdeII GATC 7 cut(s) 82, 109, 170, 917, 1001, 1067, 1395
NlaIV GGNNCC 7 cut(s) 158, 276, 541, 648, 1014, 1096, 1143
NmeAIII GCCGAG 1 cut(s) 1170
NspI RCATGY 1 cut(s) 1022
PagI TCATGA 2 cut(s) 853, 1381
PciI ACATGT 1 cut(s) 1018
PcsI WCGNNNNNNNCGW 1 cut(s) 822
PdmI GAANNNNTTC 1 cut(s) 524
PfeI GAWTC 3 cut(s) 411, 572, 1321
PkrI GCNGC 4 cut(s) 1033, 1221, 1291, 1294
PleI GAGTC 3 cut(s) 65, 302, 946
PpsI GAGTC 3 cut(s) 65, 302, 946
PscI ACATGT 1 cut(s) 1018
Psp124BI GAGCTC 1 cut(s) 471
Psp6I CCWGG 2 cut(s) 27, 1123
PspFI CCCAGC 5 cut(s) 197, 395, 415, 563, 1027
PspGI CCWGG 2 cut(s) 27, 1123
PspN4I GGNNCC 7 cut(s) 158, 276, 541, 648, 1014, 1096, 1143
PspPI GGNCC 4 cut(s) 319, 454, 647, 1277
PsuI RGATCY 1 cut(s) 1395
PvuII CAGCTG 3 cut(s) 419, 1031, 1292
RsaI GTAC 6 cut(s) 302, 360, 407, 511, 582, 1151
RsaNI GTAC 6 cut(s) 301, 359, 406, 510, 581, 1150
RseI CAYNNNNRTG 3 cut(s) 65, 252, 787
SacI GAGCTC 1 cut(s) 471
SalI GTCGAC 1 cut(s) 749
SaqAI TTAA 4 cut(s) 500, 597, 993, 1332
SatI GCNGC 4 cut(s) 1032, 1220, 1290, 1293
Sau3AI GATC 7 cut(s) 82, 109, 170, 917, 1001, 1067, 1395
Sau96I GGNCC 4 cut(s) 319, 454, 647, 1277
ScaI AGTACT 1 cut(s) 582
SchI GAGTC 3 cut(s) 66, 302, 947
ScrFI CCNGG 2 cut(s) 29, 1125
SduI GDGCHC 3 cut(s) 138, 471, 861
SfaNI GCATC 3 cut(s) 154, 1181, 1387
SinI GGWCC 3 cut(s) 319, 647, 1277
SmiMI CAYNNNNRTG 3 cut(s) 65, 252, 787
SmlI CTYRAG 2 cut(s) 464, 576
SmoI CTYRAG 2 cut(s) 464, 576
Sse9I AATT 7 cut(s) 39, 386, 520, 651, 667, 698, 1235
SsiI CCGC 3 cut(s) 953, 1370, 1406
SspMI CTAG 2 cut(s) 174, 1251
SstI GAGCTC 1 cut(s) 471
StyD4I CCNGG 2 cut(s) 27, 1123
StyI CCWWGG 3 cut(s) 270, 1098, 1401
TaiI ACGT 1 cut(s) 603
TaqI TCGA 6 cut(s) 81, 570, 750, 825, 920, 1004
TasI AATT 7 cut(s) 39, 386, 520, 651, 667, 698, 1235
TatI WGTACW 2 cut(s) 405, 580
TfiI GAWTC 3 cut(s) 411, 572, 1321
Tru1I TTAA 4 cut(s) 500, 597, 993, 1332
Tru9I TTAA 4 cut(s) 500, 597, 993, 1332
TseI GCWGC 4 cut(s) 1031, 1219, 1289, 1292
TspDTI ATGAA 5 cut(s) 280, 384, 517, 725, 1121
TspGWI ACGGA 2 cut(s) 544, 1107
VpaK11BI GGWCC 3 cut(s) 319, 647, 1277
XapI RAATTY 2 cut(s) 520, 698
XbaI TCTAGA 1 cut(s) 173
XceI RCATGY 1 cut(s) 1022
XmiI GTMKAC 1 cut(s) 750
XmnI GAANNNNTTC 1 cut(s) 524
XspI CTAG 2 cut(s) 174, 1251
ZrmI AGTACT 1 cut(s) 582
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.