Basic Information

Type: gene

Biological Identity

Species

rosa_multiflora

Chromosome

Rmu_sc0007864.1

Orthogroup ID

OG0001754

Physical Location & Seq

Genomic Coordinates Reverse (-)

28126 .. 30252

Gene Structure 2127 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rmu_sc0007864.1_g000006.1.cds

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1410 bp

atgggaaacagagtgatggtgagcctcctggttcttgtgcttgtgctttcctatggagttatggcaatggctatgaggtttaatcatgaagatgaagagtggggaagagatcagagagaagaagagtgggaagggcaccgagggcatcgccatcttttccttttgcaacaatcaaggctcctggtgaaaactgaagctggccaaatgagggtggtgagaagtgtaggtcagagaatggtagataggcatataaatgttgcctatctcaccttggaacctaactccctcttcatcccccagtacctcgactccagcttgatcctctttgtccaaagagggcaagcaaatgtgggactggtacaccgacaggaattgggggagagacaattgaaggctggggatgtgtacagaattccagcaggttccacgttttacttgcagaatgtcagggagaacaggagacttcagatcattctcagcatcgacacatctgacagtttgagaatgggtactttggagaatttcttcattggtggaggaacctacccaaaatctgtactttctgggttcgattctgagatactcacaaatgcatttaacgtctcatcttcagaaataaggcaggttttgactaggcaccaagagggtccaattatatatctaaataattcccaatcatcatcaaacctttggaccaaattcgtaagcatgaaagagcaggacagactacaagaattgaagaaaatggtggacttcccagaagaaccagagcaaaccctaacttggtcatggaagaaactgttgaactccgtgttcggaacacttgaaaatgaggagaggaagcaacagcatcatgagcactcccagaaatcctacaacctctatgatagaaagccagatttcaagaacaaccatggatggaccctctcagttgatgagtctgattactctcccctaaaggactccggcattggcgtttaccttgttagtctcaacgcagggtcaatgttggcaccacatgtgaacccaagagcaacagaatacggcgtcgttctgagaggctcaggatcactgcagattgtatttccaaatggtaccttggcaatgaaggcgaaactaaagccagggagagtgttttgggtgccaaggtatttcccatttgtccaagtggcatctaacaacgagcaacttgagatttttgggttcactacctcagcacagccgaaccggccacagtttttggctggttctagctcggtgctgcaagctctaagaggtcaagaacttgcagcggcttttggtgtgagtgagggaaggttgaatcattttgttaatgctcagcgcgaagctgtcattttgccatacaatgcagagaggaatcaaaaggcagatgggatggcagatcggtga

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Cupin_1

Protein Analysis

469

Amino Acids

53.33

Weight (kDa)

8.53

Isoelectric Point (pI)

41.38

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0001754)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G28490
fragaria_vesca	FvH4_3g33180 FvH4_3g33190 FvH4_3g33240
malus_domestica	MD03G1105100.v1.1 MD11G1130400.v1.1
prunus_persica	Prupe.6G095000_v2.0.a1
pyrus_communis	pycom11g10790 pycom11g10800
rosa_chinensis	RchiOBHm_Chr5g0060491 RchiOBHm_Chr5g0060501 RchiOBHm_Chr5g0060531 RchiOBHm_Chr5g0060591
rosa_laevigata	RLG00000035368 RLG00000035369 RLG00000035372 RLG00000035373
rosa_multiflora	Rmu_co8403353.1_g000001 Rmu_sc0001650.1_g000002 Rmu_sc0001650.1_g000006 Rmu_sc0001650.1_g000014 Rmu_sc0001650.1_g000017 Rmu_sc0007864.1_g000004 Rmu_sc0007864.1_g000006 Rmu_sc0034083.1_g000001
rosa_roxburghii	Rroxscaffold_1G00020220 Rroxscaffold_1G00020240 Rroxscaffold_1G00020280 Rroxscaffold_1G00020290
rosa_rugosa	Rorug05G0330900 Rorug05G0330900 Rorug05G0331000
rosa_samantha	Rh5AG395200 Rh5AG395300 Rh5AG395400 Rh5BG407600 Rh5BG407700 Rh5CG431300 Rh5CG431400 Rh5CG431700 Rh5CG432100 Rh5DG422400 Rh5DG422500 Rh5DG422600
rosa_wichuraiana	Rw5G037170 Rw5G037180 Rw5G037190 Rw5G037200

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc36I	ACCTGC	2 cut(s)	410, 613
Acc65I	GGTACC	1 cut(s)	1094
AccB1I	GGYRCC	5 cut(s)	135, 636, 1012, 1094, 1141
AccII	CGCG	1 cut(s)	1344
AciI	CCGC	1 cut(s)	1292
AclWI	GGATC	2 cut(s)	313, 1075
AcoI	YGGCCR	2 cut(s)	199, 1229
AcsI	RAATTY	3 cut(s)	411, 520, 698
AcuI	CTGAAG	3 cut(s)	213, 449, 594
AcyI	GRCGYC	1 cut(s)	1047
AfaI	GTAC	6 cut(s)	302, 360, 407, 511, 558, 1096
AfiI	CCNNNNNNNGG	2 cut(s)	208, 783
AflIII	ACRYGT	1 cut(s)	1018
AgsI	TTSAA	6 cut(s)	391, 739, 805, 827, 904, 1321
AjnI	CCWGG	3 cut(s)	27, 180, 1123
AluBI	AGCT	5 cut(s)	197, 315, 1254, 1268, 1349
AluI	AGCT	5 cut(s)	197, 315, 1254, 1268, 1349
Alw21I	GWGCWC	1 cut(s)	861
Alw26I	GTCTC	4 cut(s)	376, 454, 607, 995
AlwI	GGATC	2 cut(s)	313, 1075
AoxI	GGCC	2 cut(s)	199, 1229
ApeKI	GCWGC	2 cut(s)	1261, 1289
ApoI	RAATTY	3 cut(s)	411, 520, 698
Asp700I	GAANNNNTTC	1 cut(s)	524
Asp718I	GGTACC	1 cut(s)	1094
AspLEI	GCGC	1 cut(s)	1344
AspS9I	GGNCC	3 cut(s)	647, 693, 921
AsuHPI	GGTGA	4 cut(s)	31, 196, 226, 259
AvaII	GGWCC	3 cut(s)	647, 693, 921
BaeGI	GKGCMC	1 cut(s)	138
BalI	TGGCCA	1 cut(s)	201
BanI	GGYRCC	5 cut(s)	135, 636, 1012, 1094, 1141
Bbv12I	GWGCWC	1 cut(s)	861
BbvCI	CCTCAGC	1 cut(s)	1213
BbvI	GCAGC	2 cut(s)	1248, 1301
BccI	CCATC	5 cut(s)	10, 159, 912, 1385, 1390
BceAI	ACGGC	1 cut(s)	1060
BciT130I	CCWGG	3 cut(s)	29, 182, 1125
BcoDI	GTCTC	4 cut(s)	376, 454, 607, 995
BfaI	CTAG	2 cut(s)	633, 1251
BfmI	CTRYAG	1 cut(s)	1073
BfuAI	ACCTGC	2 cut(s)	410, 613
BglI	GCCNNNNNGGC	1 cut(s)	1228
BisI	GCNGC	3 cut(s)	1262, 1290, 1293
BlpI	GCTNAGC	1 cut(s)	1338
BlsI	GCNGC	3 cut(s)	1263, 1291, 1294
Bme1390I	CCNGG	3 cut(s)	29, 182, 1125
Bme18I	GGWCC	3 cut(s)	647, 693, 921
BmgT120I	GGNCC	3 cut(s)	647, 693, 921
BmrFI	CCNGG	3 cut(s)	29, 182, 1125
BmrI	ACTGGG	1 cut(s)	292
BmsI	GCATC	4 cut(s)	154, 489, 859, 1181
BmuI	ACTGGG	1 cut(s)	292
BpmI	CTGGAG	1 cut(s)	295
Bpu10I	CCTNAGC	2 cut(s)	1063, 1213
Bpu1102I	GCTNAGC	1 cut(s)	1338
BpuEI	CTTGAG	1 cut(s)	1211
BsaHI	GRCGYC	1 cut(s)	1047
BsaJI	CCNNGG	6 cut(s)	139, 270, 913, 1098, 1124, 1145
BsaXI	ACNNNNNCTCC	2 cut(s)	293, 323
Bsc4I	CCNNNNNNNGG	2 cut(s)	208, 783
Bse118I	RCCGGY	1 cut(s)	1227
Bse1I	ACTGG	2 cut(s)	298, 360
Bse3DI	GCAATG	2 cut(s)	72, 1110
BseBI	CCWGG	3 cut(s)	29, 182, 1125
BseDI	CCNNGG	6 cut(s)	139, 270, 913, 1098, 1124, 1145
BseGI	GGATG	4 cut(s)	291, 406, 923, 1401
BseLI	CCNNNNNNNGG	2 cut(s)	208, 783
BseMI	GCAATG	2 cut(s)	72, 1110
BseMII	CTCAG	7 cut(s)	490, 567, 942, 1046, 1077, 1227, 1352
BseNI	ACTGG	2 cut(s)	298, 360
BseRI	GAGGAG	1 cut(s)	848
BseSI	GKGCMC	1 cut(s)	138
BseXI	GCAGC	2 cut(s)	1248, 1301
BseYI	CCCAGC	1 cut(s)	395
Bsh1236I	CGCG	1 cut(s)	1344
BshFI	GGCC	2 cut(s)	201, 1231
BshNI	GGYRCC	5 cut(s)	135, 636, 1012, 1094, 1141
BsiHKAI	GWGCWC	1 cut(s)	861
BsiSI	CCGG	2 cut(s)	966, 1228
BslFI	GGGAC	1 cut(s)	366
BslI	CCNNNNNNNGG	2 cut(s)	208, 783
BsmAI	GTCTC	4 cut(s)	376, 454, 607, 995
BsmBI	CGTCTC	1 cut(s)	607
BsmFI	GGGAC	1 cut(s)	366
BsnI	GGCC	2 cut(s)	201, 1231
Bsp1286I	GDGCHC	2 cut(s)	138, 861
Bsp1407I	TGTACA	1 cut(s)	405
Bsp143I	GATC	5 cut(s)	109, 318, 468, 1067, 1402
Bsp1720I	GCTNAGC	1 cut(s)	1338
Bsp19I	CCATGG	1 cut(s)	913
BspACI	CCGC	1 cut(s)	1292
BspANI	GGCC	2 cut(s)	201, 1231
BspCNI	CTCAG	7 cut(s)	489, 568, 941, 1047, 1076, 1226, 1351
BspFNI	CGCG	1 cut(s)	1344
BspHI	TCATGA	2 cut(s)	85, 853
BspMAI	CTGCAG	1 cut(s)	1077
BspMI	ACCTGC	2 cut(s)	410, 613
BspPI	GGATC	2 cut(s)	313, 1075
BspT107I	GGYRCC	5 cut(s)	135, 636, 1012, 1094, 1141
BsrDI	GCAATG	2 cut(s)	72, 1110
BsrFI	RCCGGY	1 cut(s)	1227
BsrGI	TGTACA	1 cut(s)	405
BsrI	ACTGG	2 cut(s)	298, 360
BssAI	RCCGGY	1 cut(s)	1227
BssECI	CCNNGG	6 cut(s)	139, 270, 913, 1098, 1124, 1145
BssMI	GATC	5 cut(s)	109, 318, 468, 1067, 1402
BssNI	GRCGYC	1 cut(s)	1047
BssT1I	CCWWGG	4 cut(s)	270, 913, 1098, 1145
Bst2UI	CCWGG	3 cut(s)	29, 182, 1125
Bst4CI	ACNGT	3 cut(s)	497, 801, 1236
Bst6I	CTCTTC	4 cut(s)	90, 100, 117, 293
BstACI	GRCGYC	1 cut(s)	1047
BstAUI	TGTACA	1 cut(s)	405
BstC8I	GCNNGC	3 cut(s)	199, 342, 1266
BstDEI	CTNAG	8 cut(s)	476, 576, 928, 1055, 1063, 1213, 1271, 1338
BstDSI	CCRYGG	1 cut(s)	913
BstF5I	GGATG	4 cut(s)	291, 406, 923, 1401
BstFNI	CGCG	1 cut(s)	1344
BstHHI	GCGC	1 cut(s)	1344
BstKTI	GATC	5 cut(s)	112, 321, 471, 1070, 1405
BstMAI	GTCTC	4 cut(s)	376, 454, 607, 995
BstMBI	GATC	5 cut(s)	109, 318, 468, 1067, 1402
BstMWI	GCNNNNNNNGC	4 cut(s)	142, 856, 1109, 1228
BstNI	CCWGG	3 cut(s)	29, 182, 1125
BstNSI	RCATGY	1 cut(s)	1022
BstSCI	CCNGG	3 cut(s)	27, 180, 1123
BstSFI	CTRYAG	1 cut(s)	1073
BstSLI	GKGCMC	1 cut(s)	138
BstUI	CGCG	1 cut(s)	1344
BstV1I	GCAGC	2 cut(s)	1248, 1301
BsuRI	GGCC	2 cut(s)	201, 1231
BtgI	CCRYGG	1 cut(s)	913
BtgZI	GCGATG	1 cut(s)	131
BtsCI	GGATG	4 cut(s)	291, 406, 923, 1401
BtsI	GCAGTG	1 cut(s)	1070
BtsIMutI	CAGTG	1 cut(s)	1070
BveI	ACCTGC	2 cut(s)	410, 613
Cac8I	GCNNGC	3 cut(s)	199, 342, 1266
CciI	TCATGA	2 cut(s)	85, 853
CfoI	GCGC	1 cut(s)	1344
Cfr10I	RCCGGY	1 cut(s)	1227
Cfr13I	GGNCC	3 cut(s)	647, 693, 921
CseI	GACGC	1 cut(s)	1036
Csp6I	GTAC	6 cut(s)	301, 359, 406, 510, 557, 1095
CspCI	CAANNNNNGTGG	2 cut(s)	1221, 1256
CviAII	CATG	6 cut(s)	86, 709, 789, 854, 914, 1019
CviQI	GTAC	6 cut(s)	301, 359, 406, 510, 557, 1095
DdeI	CTNAG	8 cut(s)	476, 576, 928, 1055, 1063, 1213, 1271, 1338
DpnI	GATC	5 cut(s)	111, 320, 470, 1069, 1404
DpnII	GATC	5 cut(s)	109, 318, 468, 1067, 1402
EaeI	YGGCCR	2 cut(s)	199, 1229
Eam1104I	CTCTTC	4 cut(s)	90, 100, 117, 293
EarI	CTCTTC	4 cut(s)	90, 100, 117, 293
Eco130I	CCWWGG	4 cut(s)	270, 913, 1098, 1145
Eco47I	GGWCC	3 cut(s)	647, 693, 921
Eco57I	CTGAAG	3 cut(s)	213, 449, 594
EcoRI	GAATTC	1 cut(s)	411
EcoRII	CCWGG	3 cut(s)	27, 180, 1123
EcoT14I	CCWWGG	4 cut(s)	270, 913, 1098, 1145
EcoT22I	ATGCAT	1 cut(s)	595
ErhI	CCWWGG	4 cut(s)	270, 913, 1098, 1145
Esp3I	CGTCTC	1 cut(s)	607
FaeI	CATG	6 cut(s)	89, 712, 792, 857, 917, 1022
FaqI	GGGAC	1 cut(s)	366
FatI	CATG	6 cut(s)	85, 708, 788, 853, 913, 1018
Fnu4HI	GCNGC	3 cut(s)	1262, 1290, 1293
FokI	GGATG	3 cut(s)	278, 413, 930
Fsp4HI	GCNGC	3 cut(s)	1262, 1290, 1293
FspBI	CTAG	2 cut(s)	633, 1251
GlaI	GCGC	1 cut(s)	1343
GluI	GCNGC	3 cut(s)	1262, 1290, 1293
GsaI	CCCAGC	1 cut(s)	399
GsuI	CTGGAG	1 cut(s)	295
HaeIII	GGCC	2 cut(s)	201, 1231
HapII	CCGG	2 cut(s)	966, 1228
HgaI	GACGC	1 cut(s)	1036
HhaI	GCGC	1 cut(s)	1344
Hin1I	GRCGYC	1 cut(s)	1047
Hin1II	CATG	6 cut(s)	89, 712, 792, 857, 917, 1022
Hin6I	GCGC	1 cut(s)	1342
HinP1I	GCGC	1 cut(s)	1342
HinfI	GANTC	6 cut(s)	308, 572, 938, 962, 1321, 1378
HpaII	CCGG	2 cut(s)	966, 1228
HphI	GGTGA	4 cut(s)	31, 196, 226, 259
Hpy166II	GTNNAC	6 cut(s)	361, 406, 751, 979, 1024, 1206
Hpy188I	TCNGA	9 cut(s)	114, 231, 468, 493, 577, 613, 818, 943, 1056
Hpy188III	TCNNGA	5 cut(s)	86, 854, 904, 1065, 1280
Hpy8I	GTNNAC	6 cut(s)	361, 406, 751, 979, 1024, 1206
Hpy99I	CGWCG	1 cut(s)	1052
HpyAV	CCTTC	4 cut(s)	125, 385, 1102, 1308
HpyCH4III	ACNGT	3 cut(s)	497, 801, 1236
HpyCH4IV	ACGT	2 cut(s)	428, 600
HpyCH4V	TGCA	7 cut(s)	166, 439, 593, 1075, 1264, 1289, 1370
HpyF10VI	GCNNNNNNNGC	4 cut(s)	142, 856, 1109, 1228
HpyF3I	CTNAG	8 cut(s)	476, 576, 928, 1055, 1063, 1213, 1271, 1338
HpySE526I	ACGT	2 cut(s)	428, 600
Hsp92I	GRCGYC	1 cut(s)	1047
Hsp92II	CATG	6 cut(s)	89, 712, 792, 857, 917, 1022
HspAI	GCGC	1 cut(s)	1342
KpnI	GGTACC	1 cut(s)	1098
Kzo9I	GATC	5 cut(s)	109, 318, 468, 1067, 1402
LmnI	GCTCC	1 cut(s)	183
Lsp1109I	GCAGC	2 cut(s)	1248, 1301
LweI	GCATC	4 cut(s)	154, 489, 859, 1181
MaeI	CTAG	2 cut(s)	633, 1251
MaeII	ACGT	2 cut(s)	428, 600
MalI	GATC	5 cut(s)	111, 320, 470, 1069, 1404
MboI	GATC	5 cut(s)	109, 318, 468, 1067, 1402
MfeI	CAATTG	1 cut(s)	386
MhlI	GDGCHC	2 cut(s)	138, 861
MlsI	TGGCCA	1 cut(s)	201
MluCI	AATT	8 cut(s)	371, 386, 411, 520, 651, 667, 698, 734
MluNI	TGGCCA	1 cut(s)	201
MlyI	GAGTC	3 cut(s)	302, 947, 956
Mox20I	TGGCCA	1 cut(s)	201
Mph1103I	ATGCAT	1 cut(s)	595
MroXI	GAANNNNTTC	1 cut(s)	524
MscI	TGGCCA	1 cut(s)	201
MseI	TTAA	3 cut(s)	81, 597, 1332
MslI	CAYNNNNRTG	2 cut(s)	90, 252
Msp20I	TGGCCA	1 cut(s)	201
MspA1I	CMGCKG	1 cut(s)	1292
MspI	CCGG	2 cut(s)	966, 1228
MspR9I	CCNGG	3 cut(s)	29, 182, 1125
MunI	CAATTG	1 cut(s)	386
MvaI	CCWGG	3 cut(s)	29, 182, 1125
MvnI	CGCG	1 cut(s)	1344
MwoI	GCNNNNNNNGC	4 cut(s)	142, 856, 1109, 1228
NcoI	CCATGG	1 cut(s)	913
NdeII	GATC	5 cut(s)	109, 318, 468, 1067, 1402
NlaIII	CATG	6 cut(s)	89, 712, 792, 857, 917, 1022
NsiI	ATGCAT	1 cut(s)	595
NspI	RCATGY	1 cut(s)	1022
PagI	TCATGA	2 cut(s)	85, 853
PciI	ACATGT	1 cut(s)	1018
PdmI	GAANNNNTTC	1 cut(s)	524
PfeI	GAWTC	3 cut(s)	572, 1321, 1378
PkrI	GCNGC	3 cut(s)	1263, 1291, 1294
PleI	GAGTC	3 cut(s)	302, 946, 956
PpsI	GAGTC	3 cut(s)	302, 946, 956
PscI	ACATGT	1 cut(s)	1018
Psp6I	CCWGG	3 cut(s)	27, 180, 1123
PspFI	CCCAGC	1 cut(s)	395
PspGI	CCWGG	3 cut(s)	27, 180, 1123
PspPI	GGNCC	3 cut(s)	647, 693, 921
PstI	CTGCAG	1 cut(s)	1077
RsaI	GTAC	6 cut(s)	302, 360, 407, 511, 558, 1096
RsaNI	GTAC	6 cut(s)	301, 359, 406, 510, 557, 1095
RseI	CAYNNNNRTG	2 cut(s)	90, 252
SaqAI	TTAA	3 cut(s)	81, 597, 1332
SatI	GCNGC	3 cut(s)	1262, 1290, 1293
Sau3AI	GATC	5 cut(s)	109, 318, 468, 1067, 1402
Sau96I	GGNCC	3 cut(s)	647, 693, 921
SchI	GAGTC	3 cut(s)	302, 947, 956
ScrFI	CCNGG	3 cut(s)	29, 182, 1125
SduI	GDGCHC	2 cut(s)	138, 861
SfaNI	GCATC	4 cut(s)	154, 489, 859, 1181
SfcI	CTRYAG	1 cut(s)	1073
SinI	GGWCC	3 cut(s)	647, 693, 921
SmiMI	CAYNNNNRTG	2 cut(s)	90, 252
SmlI	CTYRAG	1 cut(s)	1190
SmoI	CTYRAG	1 cut(s)	1190
Sse9I	AATT	8 cut(s)	371, 386, 411, 520, 651, 667, 698, 734
SsiI	CCGC	1 cut(s)	1292
SspMI	CTAG	2 cut(s)	633, 1251
StyD4I	CCNGG	3 cut(s)	27, 180, 1123
StyI	CCWWGG	4 cut(s)	270, 913, 1098, 1145
TaaI	ACNGT	3 cut(s)	497, 801, 1236
TaiI	ACGT	2 cut(s)	431, 603
TaqI	TCGA	3 cut(s)	306, 483, 570
TasI	AATT	8 cut(s)	371, 386, 411, 520, 651, 667, 698, 734
TatI	WGTACW	2 cut(s)	405, 556
TauI	GCSGC	1 cut(s)	1295
TfiI	GAWTC	3 cut(s)	572, 1321, 1378
Tru1I	TTAA	3 cut(s)	81, 597, 1332
Tru9I	TTAA	3 cut(s)	81, 597, 1332
TscAI	CASTG	1 cut(s)	1077
TseI	GCWGC	2 cut(s)	1261, 1289
TspDTI	ATGAA	6 cut(s)	102, 108, 280, 517, 725, 1121
TspGWI	ACGGA	1 cut(s)	799
TspRI	CASTG	1 cut(s)	1077
VpaK11BI	GGWCC	3 cut(s)	647, 693, 921
XapI	RAATTY	3 cut(s)	411, 520, 698
XceI	RCATGY	1 cut(s)	1022
XmnI	GAANNNNTTC	1 cut(s)	524
XspI	CTAG	2 cut(s)	633, 1251
Zsp2I	ATGCAT	1 cut(s)	595

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.