Rmu_sc0001650.1_g000017

Vicilin-like antimicrobial peptides

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0001650.1
OG0001754
Physical Location & Seq
Forward (+)
63520 .. 66750
3231 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0001650.1_g000017.1.cds

Sequence Viewer

Length: 1647 bp
atggaaaacagagtcattagcctcctggtcattgctctcattatgtgctatggattgtcaacggccgttggattttatcgagatgaagactgggggaagcagagagaagaccccgatcaagagcaggaagagcaagagcaagaagcgatgatgggagacgaggattggttcttgttgcagaactcaaagcaggtggtgaaaactcgagctggtgaaatgagagtggtgaaaagcgtaggtgggaggattgttgataagccaatgcatattgggtttattacaatggagcccaagtccctctttgtacctcagtacatggactccagcttgatcctcttcgtccattctggggaagcaaaggtagggctgatatacagagatgaactgggtgagaggagattgagggctggggatgtgtacagaattccagctggttcgccgttttatttggtgaatacaggggagggtcagagacttcacatcatctgtagcgttgatacatctgagagtttgggaatgggtactttgcaggttacatcatcagaactaagtgatttcttgtcaaggcaacaagatggtccgattgtatatgtgactgattcacctcatccaccaagcctttggtcaagattcctagagctgaaagagcatgacagactccaaaatatgaagagaatggtgggcttcacaaaagaacctgatcaccaagaagaagaccaatcattagcatggtcatggaggaagcttctcaactctgtgttcggatcaaatgacaagagaagagacgatcccgatgcttacaagggaaaaggaaaatctcatgatgcttacaacctcttcaagagatctgcagatttcaaaaacgactatggctggagcaaggcacttgacgaatctgattactcgccactaaaagactctggcgttggtgtcttccttgtcaacctttctgcagggtcaatgatggcaccacatgtgaacccaatagcaacagagtatgggattgtattgagaggctctggcacaatacagattgtatttccaaacggaacctcggcaatgaatactaaagttcaagaaggagatgtgttttgggtcccaaggtacttcccattctgtcaaattgcatcaagaactggtcctcttgagttctttggattcaccacctcggcacgcaagaacaggccacagttcttggttggtgctagctctgttcttagggagctcagaggacctgagcttgcagctgcttttggtgtgaatgaggatcggttgagtcgcattgttcatgctcagcgtgagtccgtgatcttgccttcggtgcaagcagctcctccttacaagggggaggagagagagtcaaccccggaggaggagacagggaagcaaaccccatacaaggaggacgagaggaggcaggaagacaggaagaggcgggaagataagaagaggcgggaagacgagagggagcaatcagctgaggaagatgagagaaggcaacccccatacaaggaagatgagaggcagcagtcgtcacaggagaagcagaggaggcaacctccatacggggaaggggatgtgatgtttcagaggctcccaaaggtgatcaagagcttcgggaatgagatggttatgggttttgattga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005911 GO:0006950 GO:0006952 GO:0008150 GO:0009506 GO:0009605 GO:0009607 GO:0009620 GO:0030054 GO:0043207 GO:0050832 GO:0050896 GO:0051704 GO:0051707 GO:0055044 GO:0098542
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

548

Amino Acids

62.61

Weight (kDa)

5.52

Isoelectric Point (pI)

53.25

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 181
Acc36I ACCTGC 2 cut(s) 181, 520
AccB1I GGYRCC 1 cut(s) 967
AciI CCGC 2 cut(s) 1435, 1453
AclWI GGATC 4 cut(s) 325, 772, 782, 1275
AcoI YGGCCR 1 cut(s) 63
AcsI RAATTY 1 cut(s) 423
AfaI GTAC 5 cut(s) 306, 314, 419, 523, 1106
AfiI CCNNNNNNNGG 6 cut(s) 349, 1343, 1372, 1399, 1510, 1565
AflIII ACRYGT 1 cut(s) 973
AgsI TTSAA 3 cut(s) 841, 859, 1076
AjnI CCWGG 1 cut(s) 24
Alw21I GWGCWC 1 cut(s) 1227
Alw26I GTCTC 4 cut(s) 150, 466, 777, 1370
AlwI GGATC 4 cut(s) 325, 772, 782, 1275
Ama87I CYCGRG 1 cut(s) 204
AoxI GGCC 2 cut(s) 63, 1184
ApeKI GCWGC 4 cut(s) 1244, 1247, 1328, 1525
ApoI RAATTY 1 cut(s) 423
AspS9I GGNCC 4 cut(s) 578, 1096, 1139, 1232
AsuC2I CCSGG 1 cut(s) 1367
AsuHPI GGTGA 9 cut(s) 208, 224, 238, 401, 463, 594, 695, 1153, 1615
AsuNHI GCTAGC 1 cut(s) 1205
AvaI CYCGRG 1 cut(s) 204
AvaII GGWCC 4 cut(s) 578, 1096, 1139, 1232
BanI GGYRCC 1 cut(s) 967
BanII GRGCYC 2 cut(s) 291, 1227
BbsI GAAGAC 6 cut(s) 93, 114, 720, 925, 1428, 1464
Bbv12I GWGCWC 1 cut(s) 1227
BbvCI CCTCAGC 1 cut(s) 1479
BbvI GCAGC 4 cut(s) 1234, 1256, 1340, 1537
BccI CCATC 4 cut(s) 145, 569, 958, 1621
BceAI ACGGC 3 cut(s) 50, 78, 424
BcgI CGANNNNNNTGC 2 cut(s) 776, 810
BciT130I CCWGG 1 cut(s) 26
BclI TGATCA 2 cut(s) 700, 1605
BcnI CCSGG 1 cut(s) 1367
BcoDI GTCTC 4 cut(s) 150, 466, 777, 1370
BfaI CTAG 2 cut(s) 635, 1206
BfmI CTRYAG 3 cut(s) 487, 849, 951
BfuAI ACCTGC 2 cut(s) 181, 520
BglII AGATCT 1 cut(s) 845
BisI GCNGC 4 cut(s) 1245, 1248, 1329, 1526
BlpI GCTNAGC 1 cut(s) 1293
BlsI GCNGC 4 cut(s) 1246, 1249, 1330, 1527
Bme1390I CCNGG 2 cut(s) 26, 1367
Bme18I GGWCC 4 cut(s) 578, 1096, 1139, 1232
BmeT110I CYCGRG 1 cut(s) 204
BmgT120I GGNCC 4 cut(s) 578, 1096, 1139, 1232
BmiI GGNNCC 6 cut(s) 288, 969, 1051, 1097, 1098, 1595
BmrFI CCNGG 2 cut(s) 26, 1367
BmrI ACTGGG 2 cut(s) 100, 395
BmsI GCATC 3 cut(s) 784, 814, 1136
BmtI GCTAGC 1 cut(s) 1209
BmuI ACTGGG 2 cut(s) 100, 395
BpiI GAAGAC 6 cut(s) 93, 114, 720, 925, 1428, 1464
BplI GAGNNNNNCTC 2 cut(s) 1543, 1575
BpmI CTGGAG 2 cut(s) 307, 895
Bpu10I CCTNAGC 2 cut(s) 1236, 1479
Bpu1102I GCTNAGC 1 cut(s) 1293
BpuEI CTTGAG 1 cut(s) 1166
BpuMI CCSGG 1 cut(s) 1367
BsaJI CCNNGG 4 cut(s) 1053, 1100, 1167, 1365
BsaXI ACNNNNNCTCC 6 cut(s) 278, 305, 308, 335, 1343, 1373
Bsc4I CCNNNNNNNGG 6 cut(s) 349, 1343, 1372, 1399, 1510, 1565
Bse1I ACTGG 3 cut(s) 95, 390, 1141
Bse3DI GCAATG 2 cut(s) 30, 1065
BseBI CCWGG 1 cut(s) 26
BseDI CCNNGG 4 cut(s) 1053, 1100, 1167, 1365
BseGI GGATG 3 cut(s) 418, 607, 1582
BseLI CCNNNNNNNGG 6 cut(s) 349, 1343, 1372, 1399, 1510, 1565
BseMI GCAATG 2 cut(s) 30, 1065
BseMII CTCAG 6 cut(s) 323, 495, 1227, 1240, 1307, 1470
BseNI ACTGG 3 cut(s) 95, 390, 1141
BseRI GAGGAG 7 cut(s) 409, 1323, 1364, 1385, 1388, 1426, 1564
BseX3I CGGCCG 1 cut(s) 63
BseXI GCAGC 4 cut(s) 1234, 1256, 1340, 1537
BseYI CCCAGC 1 cut(s) 407
Bsh1285I CGRYCG 1 cut(s) 66
BshFI GGCC 2 cut(s) 65, 1186
BshNI GGYRCC 1 cut(s) 967
BsiEI CGRYCG 1 cut(s) 66
BsiHKAI GWGCWC 1 cut(s) 1227
BsiHKCI CYCGRG 1 cut(s) 204
BsiSI CCGG 1 cut(s) 1367
BslFI GGGAC 2 cut(s) 280, 1082
BslI CCNNNNNNNGG 6 cut(s) 349, 1343, 1372, 1399, 1510, 1565
BsmAI GTCTC 4 cut(s) 150, 466, 777, 1370
BsmBI CGTCTC 2 cut(s) 150, 777
BsmFI GGGAC 2 cut(s) 280, 1082
BsnI GGCC 2 cut(s) 65, 1186
BsoBI CYCGRG 1 cut(s) 204
Bsp1286I GDGCHC 2 cut(s) 291, 1227
Bsp1407I TGTACA 1 cut(s) 417
Bsp143I GATC 9 cut(s) 115, 330, 700, 764, 787, 845, 1267, 1308, 1605
Bsp1720I GCTNAGC 1 cut(s) 1293
BspACI CCGC 2 cut(s) 1435, 1453
BspANI GGCC 2 cut(s) 65, 1186
BspCNI CTCAG 6 cut(s) 322, 496, 1228, 1239, 1306, 1471
BspHI TCATGA 1 cut(s) 820
BspLI GGNNCC 6 cut(s) 288, 969, 1051, 1097, 1098, 1595
BspMAI CTGCAG 2 cut(s) 853, 955
BspMI ACCTGC 2 cut(s) 181, 520
BspOI GCTAGC 1 cut(s) 1209
BspPI GGATC 4 cut(s) 325, 772, 782, 1275
BspQI GCTCTTC 1 cut(s) 123
BspT107I GGYRCC 1 cut(s) 967
BsrDI GCAATG 2 cut(s) 30, 1065
BsrGI TGTACA 1 cut(s) 417
BsrI ACTGG 3 cut(s) 95, 390, 1141
BssECI CCNNGG 4 cut(s) 1053, 1100, 1167, 1365
BssMI GATC 9 cut(s) 115, 330, 700, 764, 787, 845, 1267, 1308, 1605
BssT1I CCWWGG 1 cut(s) 1100
Bst2UI CCWGG 1 cut(s) 26
Bst4CI ACNGT 1 cut(s) 1191
Bst6I CTCTTC 7 cut(s) 123, 341, 665, 775, 842, 1424, 1442
BstAUI TGTACA 1 cut(s) 417
BstC8I GCNNGC 4 cut(s) 1174, 1207, 1242, 1326
BstDEI CTNAG 8 cut(s) 309, 504, 548, 1217, 1226, 1236, 1293, 1479
BstF5I GGATG 3 cut(s) 418, 607, 1582
BstKTI GATC 9 cut(s) 118, 333, 703, 767, 790, 848, 1270, 1311, 1608
BstMAI GTCTC 4 cut(s) 150, 466, 777, 1370
BstMBI GATC 9 cut(s) 115, 330, 700, 764, 787, 845, 1267, 1308, 1605
BstMCI CGRYCG 1 cut(s) 66
BstMWI GCNNNNNNNGC 4 cut(s) 130, 646, 1321, 1552
BstNI CCWGG 1 cut(s) 26
BstNSI RCATGY 1 cut(s) 977
BstSCI CCNGG 2 cut(s) 24, 1365
BstSFI CTRYAG 3 cut(s) 487, 849, 951
BstV1I GCAGC 4 cut(s) 1234, 1256, 1340, 1537
BstV2I GAAGAC 6 cut(s) 93, 114, 720, 925, 1428, 1464
BstX2I RGATCY 1 cut(s) 845
BstXI CCANNNNNNTGG 1 cut(s) 621
BstYI RGATCY 1 cut(s) 845
BstZI CGGCCG 1 cut(s) 63
BsuRI GGCC 2 cut(s) 65, 1186
BtgZI GCGATG 1 cut(s) 161
BtsCI GGATG 3 cut(s) 418, 607, 1582
BveI ACCTGC 2 cut(s) 181, 520
Cac8I GCNNGC 4 cut(s) 1174, 1207, 1242, 1326
CciI TCATGA 1 cut(s) 820
Cfr13I GGNCC 4 cut(s) 578, 1096, 1139, 1232
Csp6I GTAC 5 cut(s) 305, 313, 418, 522, 1105
CspCI CAANNNNNGTGG 4 cut(s) 174, 209, 1176, 1211
CviAII CATG 7 cut(s) 316, 650, 729, 735, 821, 974, 1289
CviQI GTAC 5 cut(s) 305, 313, 418, 522, 1105
DdeI CTNAG 8 cut(s) 309, 504, 548, 1217, 1226, 1236, 1293, 1479
DpnI GATC 9 cut(s) 117, 332, 702, 766, 789, 847, 1269, 1310, 1607
DpnII GATC 9 cut(s) 115, 330, 700, 764, 787, 845, 1267, 1308, 1605
EaeI YGGCCR 1 cut(s) 63
EagI CGGCCG 1 cut(s) 63
Eam1104I CTCTTC 7 cut(s) 123, 341, 665, 775, 842, 1424, 1442
EarI CTCTTC 7 cut(s) 123, 341, 665, 775, 842, 1424, 1442
Ecl136II GAGCTC 1 cut(s) 1225
EclXI CGGCCG 1 cut(s) 63
Eco130I CCWWGG 1 cut(s) 1100
Eco24I GRGCYC 2 cut(s) 291, 1227
Eco47I GGWCC 4 cut(s) 578, 1096, 1139, 1232
Eco52I CGGCCG 1 cut(s) 63
Eco53kI GAGCTC 1 cut(s) 1225
Eco88I CYCGRG 1 cut(s) 204
EcoICRI GAGCTC 1 cut(s) 1225
EcoO109I RGGNCCY 2 cut(s) 1096, 1232
EcoRI GAATTC 1 cut(s) 423
EcoRII CCWGG 1 cut(s) 24
EcoT14I CCWWGG 1 cut(s) 1100
EcoT22I ATGCAT 1 cut(s) 267
EcoT38I GRGCYC 2 cut(s) 291, 1227
ErhI CCWWGG 1 cut(s) 1100
Esp3I CGTCTC 2 cut(s) 150, 777
FaeI CATG 7 cut(s) 319, 653, 732, 738, 824, 977, 1292
FalI AAGNNNNNCTT 2 cut(s) 284, 316
FaqI GGGAC 2 cut(s) 280, 1082
FatI CATG 7 cut(s) 315, 649, 728, 734, 820, 973, 1288
FauI CCCGC 2 cut(s) 1428, 1446
FbaI TGATCA 2 cut(s) 700, 1605
Fnu4HI GCNGC 4 cut(s) 1245, 1248, 1329, 1526
FokI GGATG 3 cut(s) 425, 594, 1589
FriOI GRGCYC 2 cut(s) 291, 1227
Fsp4HI GCNGC 4 cut(s) 1245, 1248, 1329, 1526
FspBI CTAG 2 cut(s) 635, 1206
GluI GCNGC 4 cut(s) 1245, 1248, 1329, 1526
GsaI CCCAGC 1 cut(s) 411
GsuI CTGGAG 2 cut(s) 307, 895
HaeIII GGCC 2 cut(s) 65, 1186
HapII CCGG 1 cut(s) 1367
Hin1II CATG 7 cut(s) 319, 653, 732, 738, 824, 977, 1292
HincII GTYRAC 3 cut(s) 60, 943, 1362
HindII GTYRAC 3 cut(s) 60, 943, 1362
HindIII AAGCTT 1 cut(s) 743
HpaII CCGG 1 cut(s) 1367
HphI GGTGA 9 cut(s) 208, 224, 238, 401, 463, 594, 695, 1153, 1615
Hpy166II GTNNAC 5 cut(s) 60, 418, 943, 979, 1362
Hpy188I TCNGA 8 cut(s) 471, 505, 544, 582, 764, 898, 1229, 1590
Hpy8I GTNNAC 5 cut(s) 60, 418, 943, 979, 1362
HpyAV CCTTC 4 cut(s) 1073, 1326, 1488, 1565
HpyCH4III ACNGT 1 cut(s) 1191
HpyCH4V TGCA 8 cut(s) 178, 265, 529, 851, 953, 1127, 1244, 1324
HpyF10VI GCNNNNNNNGC 4 cut(s) 130, 646, 1321, 1552
HpyF3I CTNAG 8 cut(s) 309, 504, 548, 1217, 1226, 1236, 1293, 1479
Hsp92II CATG 7 cut(s) 319, 653, 732, 738, 824, 977, 1292
KflI GGGWCCC 1 cut(s) 1096
Ksp22I TGATCA 2 cut(s) 700, 1605
Kzo9I GATC 9 cut(s) 115, 330, 700, 764, 787, 845, 1267, 1308, 1605
LguI GCTCTTC 1 cut(s) 123
LmnI GCTCC 6 cut(s) 286, 876, 1222, 1336, 1468, 1599
Lsp1109I GCAGC 4 cut(s) 1234, 1256, 1340, 1537
LweI GCATC 3 cut(s) 784, 814, 1136
MaeI CTAG 2 cut(s) 635, 1206
MaeIII GTNAC 3 cut(s) 532, 592, 1533
MalI GATC 9 cut(s) 117, 332, 702, 766, 789, 847, 1269, 1310, 1607
MboI GATC 9 cut(s) 115, 330, 700, 764, 787, 845, 1267, 1308, 1605
MflI RGATCY 1 cut(s) 845
MhlI GDGCHC 2 cut(s) 291, 1227
MluCI AATT 2 cut(s) 423, 1122
MlyI GAGTC 7 cut(s) 21, 314, 651, 911, 1285, 1310, 1367
MmeI TCCRAC 1 cut(s) 49
Mph1103I ATGCAT 1 cut(s) 267
MslI CAYNNNNRTG 2 cut(s) 727, 733
MspA1I CMGCKG 3 cut(s) 431, 1247, 1478
MspI CCGG 1 cut(s) 1367
MspR9I CCNGG 2 cut(s) 26, 1367
MvaI CCWGG 1 cut(s) 26
MwoI GCNNNNNNNGC 4 cut(s) 130, 646, 1321, 1552
NciI CCSGG 1 cut(s) 1367
NdeII GATC 9 cut(s) 115, 330, 700, 764, 787, 845, 1267, 1308, 1605
NheI GCTAGC 1 cut(s) 1205
NlaIII CATG 7 cut(s) 319, 653, 732, 738, 824, 977, 1292
NlaIV GGNNCC 6 cut(s) 288, 969, 1051, 1097, 1098, 1595
NmeAIII GCCGAG 2 cut(s) 1034, 1148
NmuCI GTSAC 2 cut(s) 592, 1533
NsiI ATGCAT 1 cut(s) 267
NspI RCATGY 1 cut(s) 977
PaeR7I CTCGAG 1 cut(s) 204
PagI TCATGA 1 cut(s) 820
PaqCI CACCTGC 1 cut(s) 181
PciI ACATGT 1 cut(s) 973
PciSI GCTCTTC 1 cut(s) 123
PfeI GAWTC 4 cut(s) 599, 630, 893, 1158
PkrI GCNGC 4 cut(s) 1246, 1249, 1330, 1527
PleI GAGTC 7 cut(s) 20, 314, 651, 911, 1284, 1309, 1366
PpsI GAGTC 7 cut(s) 20, 314, 651, 911, 1284, 1309, 1366
PpuMI RGGWCCY 2 cut(s) 1096, 1232
PscI ACATGT 1 cut(s) 973
Psp124BI GAGCTC 1 cut(s) 1227
Psp5II RGGWCCY 2 cut(s) 1096, 1232
Psp6I CCWGG 1 cut(s) 24
PspFI CCCAGC 1 cut(s) 407
PspGI CCWGG 1 cut(s) 24
PspN4I GGNNCC 6 cut(s) 288, 969, 1051, 1097, 1098, 1595
PspPI GGNCC 4 cut(s) 578, 1096, 1139, 1232
PspPPI RGGWCCY 2 cut(s) 1096, 1232
PspXI VCTCGAGB 1 cut(s) 204
PstI CTGCAG 2 cut(s) 853, 955
PsuI RGATCY 1 cut(s) 845
PvuII CAGCTG 3 cut(s) 431, 1247, 1478
RsaI GTAC 5 cut(s) 306, 314, 419, 523, 1106
RsaNI GTAC 5 cut(s) 305, 313, 418, 522, 1105
RseI CAYNNNNRTG 2 cut(s) 727, 733
SacI GAGCTC 1 cut(s) 1227
SapI GCTCTTC 1 cut(s) 123
SatI GCNGC 4 cut(s) 1245, 1248, 1329, 1526
Sau3AI GATC 9 cut(s) 115, 330, 700, 764, 787, 845, 1267, 1308, 1605
Sau96I GGNCC 4 cut(s) 578, 1096, 1139, 1232
SchI GAGTC 7 cut(s) 21, 314, 651, 911, 1285, 1310, 1367
ScrFI CCNGG 2 cut(s) 26, 1367
SduI GDGCHC 2 cut(s) 291, 1227
SfaNI GCATC 3 cut(s) 784, 814, 1136
SfcI CTRYAG 3 cut(s) 487, 849, 951
Sfr274I CTCGAG 1 cut(s) 204
SinI GGWCC 4 cut(s) 578, 1096, 1139, 1232
SlaI CTCGAG 1 cut(s) 204
SmiMI CAYNNNNRTG 2 cut(s) 727, 733
SmlI CTYRAG 2 cut(s) 204, 1145
SmoI CTYRAG 2 cut(s) 204, 1145
Sse9I AATT 2 cut(s) 423, 1122
SsiI CCGC 2 cut(s) 1435, 1453
SspMI CTAG 2 cut(s) 635, 1206
SstI GAGCTC 1 cut(s) 1227
StyD4I CCNGG 2 cut(s) 24, 1365
StyI CCWWGG 1 cut(s) 1100
TaaI ACNGT 1 cut(s) 1191
TaqI TCGA 2 cut(s) 79, 205
TasI AATT 2 cut(s) 423, 1122
TatI WGTACW 2 cut(s) 312, 417
TfiI GAWTC 4 cut(s) 599, 630, 893, 1158
TseFI GTSAC 2 cut(s) 592, 1533
TseI GCWGC 4 cut(s) 1244, 1247, 1328, 1525
Tsp45I GTSAC 2 cut(s) 592, 1533
TspDTI ATGAA 5 cut(s) 99, 396, 683, 1076, 1277
TspGWI ACGGA 2 cut(s) 1062, 1294
VpaK11BI GGWCC 4 cut(s) 578, 1096, 1139, 1232
XapI RAATTY 1 cut(s) 423
XceI RCATGY 1 cut(s) 977
XcmI CCANNNNNNNNNTGG 1 cut(s) 618
XhoI CTCGAG 1 cut(s) 204
XspI CTAG 2 cut(s) 635, 1206
Zsp2I ATGCAT 1 cut(s) 267
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.