AT2G33590

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002886
Physical Location & Seq
Forward (+)
14224268 .. 14226636
2369 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33590.1

Sequence Viewer

Length: 966 bp
ATGGCTGATGTTCACAAAGGAAAGGTTTGTGTAACCGGTGCCGGAGGGTTTTTGGGTTCGTGGGTCGTCGATCTTCTTCTCTCTAAGGATTACTTCGTCCATGGCACTGTCAGAGACCCTGATAATGAAAAGTATGCTCATTTGAAGAAGCTCGAGAAAGCTGGTGACAAACTCAAGCTCTTCAAGGCTGATTTGTTGGACTACGGCTCTCTTCAATCTGCTATTGCAGGTTGCAGCGGAGTCTTCCATGTCGCTTGCCCGGTTCCACCAGCTTCAGTCCCTAATCCTGAGGTAGAATTGATCGCACCTGCTGTGGATGGCACGCTGAATGTGCTTAAGGCATGCATTGAAGCGAATGTTAAGCGTGTTGTGTATGTTTCCTCTGTAGCTGCGGCTTTCATGAACCCTATGTGGTCAAAGAATCAAGTATTAGATGAGGCATGTTGGTCTGACCAGGAATACTGCAAGAAAACTGAGAATTGGTATTGTCTGGCAAAGACACGAGCAGAGAGTGAAGCTTTTGAATTTGCAAAACGAACCGGACTTCATCTCGTCAGTGTTTGCCCTACCCTTGTTTTGGGACCTATACTGCAGCAGAACACAGTAAATGCTAGTAGCCTGGTTCTTCTTAAGCTTCTTAAAGAGGGTTTTGAAACACGTGATAACCAAGAACGTCATTTGGTAGATGTGCGTGATGTAGCTCAAGCTTTACTCTTGGTTTATGAGAAAGCAGAAGCTGAAGGAAGATACATATGTACATCCCACACGGTTAAAGAAGAGATTGTGGTTGAGAAGCTGAAGAGTTTTTATCCACATTACAACTACCCAAAGAAATACATTGATGCGGAAGATAGAGTGAAGGTGAGTTCAGAGAAATTGCAGAAGTTGGGTTGGACTTACCGGCCGTTGGAGGAAACACTTGTGGATTCTGTTGAGAGCTACCGGAAAGCTAAGCTTGTGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

321

Amino Acids

35.87

Weight (kDa)

6.47

Isoelectric Point (pI)

31.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 129 3.1e-10 NmrA-like family
KR PF08659 7 - 137 3.5e-07 KR domain
Epimerase PF01370 9 - 246 6.4e-27 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 137 8.3e-09 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 11 - 239 1.9e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 292 2.2e-18 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 194 8.5e-08 Male sterility protein
NAD_binding_10 PF13460 13 - 133 2.5e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 316
Acc36I ACCTGC 2 cut(s) 218, 316
AccB1I GGYRCC 1 cut(s) 38
AciI CCGC 3 cut(s) 237, 392, 845
AcoI YGGCCR 1 cut(s) 902
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 3 cut(s) 258, 759, 818
AcvI CACGTG 1 cut(s) 659
AfaI GTAC 1 cut(s) 757
AfiI CCNNNNNNNGG 2 cut(s) 577, 907
AflII CTTAAG 2 cut(s) 335, 629
AflIII ACRYGT 1 cut(s) 656
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 6 cut(s) 145, 184, 215, 350, 524, 653
AjnI CCWGG 2 cut(s) 453, 618
Alw26I GTCTC 1 cut(s) 108
AlwNI CAGNNNCTG 1 cut(s) 737
Ama87I CYCGRG 1 cut(s) 152
AoxI GGCC 1 cut(s) 902
ApeKI GCWGC 3 cut(s) 234, 389, 592
ApoI RAATTY 1 cut(s) 524
AsiGI ACCGGT 1 cut(s) 35
AspS9I GGNCC 1 cut(s) 581
AsuC2I CCSGG 1 cut(s) 260
AsuHPI GGTGA 2 cut(s) 176, 874
AvaI CYCGRG 1 cut(s) 152
AvaII GGWCC 1 cut(s) 581
AxyI CCTNAGG 1 cut(s) 288
BaeI ACNNNNGTAYC 2 cut(s) 739, 772
BanI GGYRCC 1 cut(s) 38
BauI CACGAG 1 cut(s) 501
BbrPI CACGTG 1 cut(s) 659
BbsI GAAGAC 1 cut(s) 235
BbvI GCAGC 3 cut(s) 246, 376, 604
BccI CCATC 1 cut(s) 311
BceAI ACGGC 2 cut(s) 220, 889
BciT130I CCWGG 2 cut(s) 455, 620
BcnI CCSGG 1 cut(s) 260
BcoDI GTCTC 1 cut(s) 108
BfaI CTAG 1 cut(s) 612
BfmI CTRYAG 2 cut(s) 384, 590
BfrI CTTAAG 2 cut(s) 335, 629
BfuAI ACCTGC 2 cut(s) 218, 316
BisI GCNGC 4 cut(s) 235, 390, 393, 593
BlpI GCTNAGC 1 cut(s) 951
BlsI GCNGC 4 cut(s) 236, 391, 394, 594
Bme1390I CCNGG 3 cut(s) 260, 455, 620
Bme18I GGWCC 1 cut(s) 581
BmeT110I CYCGRG 1 cut(s) 152
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 3 cut(s) 40, 264, 582
BmrFI CCNGG 3 cut(s) 260, 455, 620
BmsI GCATC 1 cut(s) 832
BpiI GAAGAC 1 cut(s) 235
Bpu1102I GCTNAGC 1 cut(s) 951
BpuEI CTTGAG 2 cut(s) 158, 687
BpuMI CCSGG 1 cut(s) 260
BsaAI YACGTR 1 cut(s) 659
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 1 cut(s) 100
BsaWI WCCGGW 3 cut(s) 35, 539, 942
Bsc4I CCNNNNNNNGG 2 cut(s) 577, 907
Bse118I RCCGGY 2 cut(s) 35, 900
Bse21I CCTNAGG 1 cut(s) 288
BseBI CCWGG 2 cut(s) 455, 620
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 2 cut(s) 322, 758
BseLI CCNNNNNNNGG 2 cut(s) 577, 907
BseMII CTCAG 2 cut(s) 279, 465
BseX3I CGGCCG 1 cut(s) 902
BseXI GCAGC 3 cut(s) 246, 376, 604
Bsh1285I CGRYCG 1 cut(s) 905
BshFI GGCC 1 cut(s) 904
BshNI GGYRCC 1 cut(s) 38
BshTI ACCGGT 1 cut(s) 35
BsiEI CGRYCG 1 cut(s) 905
BsiHKCI CYCGRG 1 cut(s) 152
BsiSI CCGG 6 cut(s) 36, 42, 260, 540, 901, 943
BslFI GGGAC 2 cut(s) 263, 594
BslI CCNNNNNNNGG 2 cut(s) 577, 907
BsmAI GTCTC 1 cut(s) 108
BsmFI GGGAC 2 cut(s) 263, 594
BsnI GGCC 1 cut(s) 904
Bso31I GGTCTC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 152
Bsp1407I TGTACA 1 cut(s) 755
Bsp143I GATC 2 cut(s) 70, 300
Bsp1720I GCTNAGC 1 cut(s) 951
Bsp19I CCATGG 1 cut(s) 100
BspACI CCGC 3 cut(s) 237, 392, 845
BspANI GGCC 1 cut(s) 904
BspCNI CTCAG 2 cut(s) 280, 466
BspHI TCATGA 1 cut(s) 399
BspLI GGNNCC 3 cut(s) 40, 264, 582
BspMAI CTGCAG 1 cut(s) 594
BspMI ACCTGC 2 cut(s) 218, 316
BspQI GCTCTTC 1 cut(s) 185
BspT107I GGYRCC 1 cut(s) 38
BspTI CTTAAG 2 cut(s) 335, 629
BspTNI GGTCTC 1 cut(s) 108
BsrFI RCCGGY 2 cut(s) 35, 900
BsrGI TGTACA 1 cut(s) 755
BssAI RCCGGY 2 cut(s) 35, 900
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 2 cut(s) 70, 300
BssSI CACGAG 1 cut(s) 501
BssT1I CCWWGG 1 cut(s) 100
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 2 cut(s) 455, 620
Bst4CI ACNGT 3 cut(s) 109, 604, 769
Bst6I CTCTTC 4 cut(s) 185, 216, 771, 794
BstAFI CTTAAG 2 cut(s) 335, 629
BstAUI TGTACA 1 cut(s) 755
BstBAI YACGTR 1 cut(s) 659
BstC8I GCNNGC 3 cut(s) 256, 323, 343
BstDEI CTNAG 4 cut(s) 84, 288, 474, 951
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 2 cut(s) 322, 758
BstKTI GATC 2 cut(s) 73, 303
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 2 cut(s) 70, 300
BstMCI CGRYCG 1 cut(s) 905
BstMWI GCNNNNNNNGC 1 cut(s) 331
BstNI CCWGG 2 cut(s) 455, 620
BstNSI RCATGY 2 cut(s) 345, 444
BstSCI CCNGG 3 cut(s) 258, 453, 618
BstSFI CTRYAG 2 cut(s) 384, 590
BstV1I GCAGC 3 cut(s) 246, 376, 604
BstV2I GAAGAC 1 cut(s) 235
BstZI CGGCCG 1 cut(s) 902
Bsu36I CCTNAGG 1 cut(s) 288
BsuRI GGCC 1 cut(s) 904
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 2 cut(s) 322, 758
BtsIMutI CAGTG 2 cut(s) 105, 562
BveI ACCTGC 2 cut(s) 218, 316
Cac8I GCNNGC 3 cut(s) 256, 323, 343
CaiI CAGNNNCTG 1 cut(s) 737
CciI TCATGA 1 cut(s) 399
Cfr10I RCCGGY 2 cut(s) 35, 900
Cfr13I GGNCC 1 cut(s) 581
Csp6I GTAC 1 cut(s) 756
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 5 cut(s) 101, 248, 342, 400, 441
CviQI GTAC 1 cut(s) 756
DdeI CTNAG 4 cut(s) 84, 288, 474, 951
DpnI GATC 2 cut(s) 72, 302
DpnII GATC 2 cut(s) 70, 300
EaeI YGGCCR 1 cut(s) 902
EagI CGGCCG 1 cut(s) 902
Eam1104I CTCTTC 4 cut(s) 185, 216, 771, 794
EarI CTCTTC 4 cut(s) 185, 216, 771, 794
EclXI CGGCCG 1 cut(s) 902
Eco130I CCWWGG 1 cut(s) 100
Eco31I GGTCTC 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 581
Eco52I CGGCCG 1 cut(s) 902
Eco57I CTGAAG 3 cut(s) 258, 759, 818
Eco72I CACGTG 1 cut(s) 659
Eco81I CCTNAGG 1 cut(s) 288
Eco88I CYCGRG 1 cut(s) 152
EcoO109I RGGNCCY 1 cut(s) 581
EcoRII CCWGG 2 cut(s) 453, 618
EcoT14I CCWWGG 1 cut(s) 100
EcoT22I ATGCAT 1 cut(s) 347
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 5 cut(s) 104, 251, 345, 403, 444
FalI AAGNNNNNCTT 3 cut(s) 77, 109, 939
FaqI GGGAC 2 cut(s) 263, 594
FatI CATG 5 cut(s) 100, 247, 341, 399, 440
FauNDI CATATG 1 cut(s) 752
Fnu4HI GCNGC 4 cut(s) 235, 390, 393, 593
FokI GGATG 2 cut(s) 329, 745
Fsp4HI GCNGC 4 cut(s) 235, 390, 393, 593
FspBI CTAG 1 cut(s) 612
GluI GCNGC 4 cut(s) 235, 390, 393, 593
HaeIII GGCC 1 cut(s) 904
HapII CCGG 6 cut(s) 36, 42, 260, 540, 901, 943
Hin1II CATG 5 cut(s) 104, 251, 345, 403, 444
HindIII AAGCTT 4 cut(s) 516, 632, 705, 953
HinfI GANTC 3 cut(s) 240, 421, 926
HpaII CCGG 6 cut(s) 36, 42, 260, 540, 901, 943
HphI GGTGA 2 cut(s) 176, 874
Hpy166II GTNNAC 2 cut(s) 13, 961
Hpy188I TCNGA 3 cut(s) 113, 451, 871
Hpy188III TCNNGA 3 cut(s) 154, 287, 400
Hpy8I GTNNAC 2 cut(s) 13, 961
Hpy99I CGWCG 1 cut(s) 71
HpyAV CCTTC 2 cut(s) 734, 853
HpyCH4III ACNGT 3 cut(s) 109, 604, 769
HpyCH4IV ACGT 2 cut(s) 658, 673
HpyCH4V TGCA 7 cut(s) 227, 234, 345, 465, 530, 592, 880
HpyF10VI GCNNNNNNNGC 1 cut(s) 331
HpyF3I CTNAG 4 cut(s) 84, 288, 474, 951
HpySE526I ACGT 2 cut(s) 658, 673
Hsp92II CATG 5 cut(s) 104, 251, 345, 403, 444
Kzo9I GATC 2 cut(s) 70, 300
LguI GCTCTTC 1 cut(s) 185
Lsp1109I GCAGC 3 cut(s) 246, 376, 604
LweI GCATC 1 cut(s) 832
MaeI CTAG 1 cut(s) 612
MaeII ACGT 2 cut(s) 658, 673
MaeIII GTNAC 2 cut(s) 31, 164
MalI GATC 2 cut(s) 72, 302
MboI GATC 2 cut(s) 70, 300
MluCI AATT 4 cut(s) 296, 478, 524, 875
MlyI GAGTC 1 cut(s) 249
MmeI TCCRAC 3 cut(s) 177, 872, 888
MnlI CCTC 6 cut(s) 38, 283, 391, 430, 637, 904
Mph1103I ATGCAT 1 cut(s) 347
MseI TTAA 5 cut(s) 336, 360, 630, 639, 771
MspA1I CMGCKG 1 cut(s) 237
MspCI CTTAAG 2 cut(s) 335, 629
MspI CCGG 6 cut(s) 36, 42, 260, 540, 901, 943
MspR9I CCNGG 3 cut(s) 260, 455, 620
MvaI CCWGG 2 cut(s) 455, 620
MwoI GCNNNNNNNGC 1 cut(s) 331
NciI CCSGG 1 cut(s) 260
NcoI CCATGG 1 cut(s) 100
NdeI CATATG 1 cut(s) 752
NdeII GATC 2 cut(s) 70, 300
NlaIII CATG 5 cut(s) 104, 251, 345, 403, 444
NlaIV GGNNCC 3 cut(s) 40, 264, 582
NmuCI GTSAC 1 cut(s) 164
NsiI ATGCAT 1 cut(s) 347
NspI RCATGY 2 cut(s) 345, 444
PaeI GCATGC 1 cut(s) 345
PaeR7I CTCGAG 1 cut(s) 152
PagI TCATGA 1 cut(s) 399
PaqCI CACCTGC 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 185
PfeI GAWTC 2 cut(s) 421, 926
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 4 cut(s) 236, 391, 394, 594
PleI GAGTC 1 cut(s) 248
PmaCI CACGTG 1 cut(s) 659
PmlI CACGTG 1 cut(s) 659
PpsI GAGTC 1 cut(s) 248
Ppu21I YACGTR 1 cut(s) 659
PpuMI RGGWCCY 1 cut(s) 581
Psp5II RGGWCCY 1 cut(s) 581
Psp6I CCWGG 2 cut(s) 453, 618
PspCI CACGTG 1 cut(s) 659
PspGI CCWGG 2 cut(s) 453, 618
PspN4I GGNNCC 3 cut(s) 40, 264, 582
PspPI GGNCC 1 cut(s) 581
PspPPI RGGWCCY 1 cut(s) 581
PstI CTGCAG 1 cut(s) 594
PstNI CAGNNNCTG 1 cut(s) 737
RsaI GTAC 1 cut(s) 757
RsaNI GTAC 1 cut(s) 756
SapI GCTCTTC 1 cut(s) 185
SaqAI TTAA 5 cut(s) 336, 360, 630, 639, 771
SatI GCNGC 4 cut(s) 235, 390, 393, 593
Sau3AI GATC 2 cut(s) 70, 300
Sau96I GGNCC 1 cut(s) 581
SchI GAGTC 1 cut(s) 249
ScrFI CCNGG 3 cut(s) 260, 455, 620
SfaNI GCATC 1 cut(s) 832
SfcI CTRYAG 2 cut(s) 384, 590
Sfr274I CTCGAG 1 cut(s) 152
SinI GGWCC 1 cut(s) 581
SlaI CTCGAG 1 cut(s) 152
SmlI CTYRAG 5 cut(s) 152, 173, 335, 629, 702
SmoI CTYRAG 5 cut(s) 152, 173, 335, 629, 702
SphI GCATGC 1 cut(s) 345
Sse9I AATT 4 cut(s) 296, 478, 524, 875
SsiI CCGC 3 cut(s) 237, 392, 845
SspMI CTAG 1 cut(s) 612
StyD4I CCNGG 3 cut(s) 258, 453, 618
StyI CCWWGG 1 cut(s) 100
TaaI ACNGT 3 cut(s) 109, 604, 769
TaiI ACGT 2 cut(s) 661, 676
TaqI TCGA 2 cut(s) 69, 153
TasI AATT 4 cut(s) 296, 478, 524, 875
TatI WGTACW 1 cut(s) 755
TauI GCSGC 1 cut(s) 395
TfiI GAWTC 2 cut(s) 421, 926
Tru1I TTAA 5 cut(s) 336, 360, 630, 639, 771
Tru9I TTAA 5 cut(s) 336, 360, 630, 639, 771
TscAI CASTG 2 cut(s) 112, 562
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 3 cut(s) 234, 389, 592
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 4 cut(s) 141, 388, 416, 536
TspRI CASTG 2 cut(s) 112, 562
Vha464I CTTAAG 2 cut(s) 335, 629
VpaK11BI GGWCC 1 cut(s) 581
XapI RAATTY 1 cut(s) 524
XceI RCATGY 2 cut(s) 345, 444
XhoI CTCGAG 1 cut(s) 152
XspI CTAG 1 cut(s) 612
Zsp2I ATGCAT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.