Rroxscaffold_3G00242880

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002886
Physical Location & Seq
Forward (+)
35402010 .. 35405190
3181 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242880.1

Sequence Viewer

Length: 1035 bp
ATGGCAGTTCAGAATGGAAGGTTCTGTGTTACAGGGGCTGGAGGCTTCGTTGGCTCCTGGGTCGTTAAGCTTCTTCTTTCCAAGAACTGCATAGTCCACGGAACCGTCAGAGAACCCACGGATGACAAGAACTCTCACTTGAGAAAGCTTGACAAGGCATCTGAGAACCTGAAACTATTCAAGGCAGACTTATTGGATTATGACTCCCTTCGCTCGGCAATTGAAGGATGTGATGGAGTATTTCATGTTGCCAGTCCTGCTCCCTCCGGCAGTGTTCCAAATCCTGAGGCAACATCTAAAGGGTGCCAACGTATTTGTAGCCGTTGCTGTCCCATCACTATTCAGCGCTCAAAGTACACATGGATTGAGTTAATTGAACCTGCTGTTAAGGGGACACTTAATGTACTTAAAGCATCACTTGAAACAAAAGTCAAGCGAGTTGTTTCTGTGTCCTCTATTGCTGCTGTGGCCGCGAATCCTACGTTTGCTGAAGGTCAAGTGTTGGATGAGTCTTGTTGGTCAGATAAGGAATACTGCAGAAATACTGAGAACTGGTATTGCCTTTCAAAAACTGAAGCAGAATCTGAGGCTCTGCAGTTTGCAAAAACAACCGGGATTGATCTTGTGACTGTTTGTCCTACACTTATACTTGGCCCAATTCTGCAGTCGACTGTAAATGCAAGTACCTTCATCTTCATCAAGCTTTTGAAGGAAGGGAAGGAGTCACTGGAAAACAGGCCTCAGAAGATAGTTGACGTACGGGATTTAGCTGAAGCACTTCTTATGGCATATGAGAAGCCGGAGGCTGAAGGAAGATACATATGCATGTCCCACAATGTTAAGAATGGGGATCTAGTTGAGAAGCTGAGGCGCCTTTATCCTAATTACAATTATCCGAAGAACTTTGTTGAAGTAGGCAAAGACAGACAGGTGAGCTCAGAGAAATTGCAAAGGCTAGGTTGGAGTTACCGGTCATTGGACGATACTCTCATTGACTCTGTTGAAAGCTACCGGGAGGCTGGACTATTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

344

Amino Acids

38.2

Weight (kDa)

6.89

Isoelectric Point (pI)

37.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 84 4.9e-07 NmrA-like family
Epimerase PF01370 9 - 88 4.2e-11 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 116 1.3e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 98 4.9e-12 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 161 1.4e-12 NAD(P)H-binding
Epimerase PF01370 116 - 270 2.4e-11 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 123 - 227 6.4e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 388
AccB1I GGYRCC 2 cut(s) 303, 870
AccI GTMKAC 1 cut(s) 668
AccII CGCG 1 cut(s) 473
AciI CCGC 1 cut(s) 471
AclWI GGATC 1 cut(s) 858
AcoI YGGCCR 1 cut(s) 468
AcuI CTGAAG 4 cut(s) 510, 594, 792, 828
AcyI GRCGYC 1 cut(s) 871
AfaI GTAC 4 cut(s) 356, 405, 685, 759
AfeI AGCGCT 1 cut(s) 347
AfiI CCNNNNNNNGG 2 cut(s) 214, 976
AgeI ACCGGT 1 cut(s) 969
AgsI TTSAA 8 cut(s) 181, 224, 377, 422, 567, 709, 911, 1004
AhdI GACNNNNNGTC 1 cut(s) 633
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 7 cut(s) 70, 148, 703, 770, 865, 936, 1008
AluI AGCT 7 cut(s) 70, 148, 703, 770, 865, 936, 1008
Alw21I GWGCWC 1 cut(s) 938
AlwI GGATC 1 cut(s) 858
AlwNI CAGNNNCTG 2 cut(s) 38, 584
Aor51HI AGCGCT 1 cut(s) 347
AoxI GGCC 3 cut(s) 468, 652, 737
ApeKI GCWGC 1 cut(s) 461
AsiGI ACCGGT 1 cut(s) 969
Asp700I GAANNNNTTC 2 cut(s) 176, 777
AspLEI GCGC 2 cut(s) 348, 873
AspS9I GGNCC 1 cut(s) 653
AsuC2I CCSGG 2 cut(s) 613, 1013
AsuHPI GGTGA 1 cut(s) 943
AxyI CCTNAGG 1 cut(s) 285
BanI GGYRCC 2 cut(s) 303, 870
BanII GRGCYC 1 cut(s) 938
Bbv12I GWGCWC 1 cut(s) 938
BbvCI CCTCAGC 1 cut(s) 866
BbvI GCAGC 1 cut(s) 448
BccI CCATC 2 cut(s) 227, 341
BceAI ACGGC 1 cut(s) 306
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 2 cut(s) 613, 1013
BfaI CTAG 2 cut(s) 854, 956
BfmI CTRYAG 3 cut(s) 535, 593, 662
BfoI RGCGCY 2 cut(s) 349, 874
BfuAI ACCTGC 1 cut(s) 388
BisI GCNGC 2 cut(s) 462, 471
BlsI GCNGC 2 cut(s) 463, 472
Bme1390I CCNGG 3 cut(s) 58, 613, 1013
BmeRI GACNNNNNGTC 1 cut(s) 633
BmgT120I GGNCC 1 cut(s) 653
BmiI GGNNCC 4 cut(s) 55, 103, 305, 872
BmrFI CCNGG 3 cut(s) 58, 613, 1013
BmsI GCATC 2 cut(s) 167, 422
BpmI CTGGAG 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 866
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 2 cut(s) 613, 1013
BsaHI GRCGYC 1 cut(s) 871
BsaJI CCNNGG 3 cut(s) 57, 97, 117
BsaWI WCCGGW 1 cut(s) 969
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 976
Bse118I RCCGGY 1 cut(s) 969
Bse1I ACTGG 3 cut(s) 252, 557, 732
Bse21I CCTNAGG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 3 cut(s) 57, 97, 117
BseGI GGATG 3 cut(s) 127, 233, 511
BseLI CCNNNNNNNGG 2 cut(s) 214, 976
BseMII CTCAG 7 cut(s) 153, 276, 537, 576, 755, 857, 951
BseNI ACTGG 3 cut(s) 252, 557, 732
BseXI GCAGC 1 cut(s) 448
Bsh1236I CGCG 1 cut(s) 473
BshFI GGCC 3 cut(s) 470, 654, 739
BshNI GGYRCC 2 cut(s) 303, 870
BshTI ACCGGT 1 cut(s) 969
BsiHKAI GWGCWC 1 cut(s) 938
BsiSI CCGG 5 cut(s) 267, 612, 800, 970, 1012
BsiWI CGTACG 1 cut(s) 757
BslFI GGGAC 3 cut(s) 315, 406, 814
BslI CCNNNNNNNGG 2 cut(s) 214, 976
BsmFI GGGAC 3 cut(s) 315, 406, 814
BsnI GGCC 3 cut(s) 470, 654, 739
Bsp1286I GDGCHC 1 cut(s) 938
Bsp143I GATC 2 cut(s) 619, 850
BspACI CCGC 1 cut(s) 471
BspANI GGCC 3 cut(s) 470, 654, 739
BspCNI CTCAG 7 cut(s) 154, 277, 538, 577, 754, 858, 950
BspFNI CGCG 1 cut(s) 473
BspLI GGNNCC 4 cut(s) 55, 103, 305, 872
BspMAI CTGCAG 3 cut(s) 539, 597, 666
BspMI ACCTGC 1 cut(s) 388
BspPI GGATC 1 cut(s) 858
BspT107I GGYRCC 2 cut(s) 303, 870
BsrFI RCCGGY 1 cut(s) 969
BsrI ACTGG 3 cut(s) 252, 557, 732
BssAI RCCGGY 1 cut(s) 969
BssECI CCNNGG 3 cut(s) 57, 97, 117
BssMI GATC 2 cut(s) 619, 850
BssNI GRCGYC 1 cut(s) 871
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 3 cut(s) 106, 631, 673
BstACI GRCGYC 1 cut(s) 871
BstDEI CTNAG 7 cut(s) 162, 285, 546, 585, 741, 866, 937
BstDSI CCRYGG 2 cut(s) 97, 117
BstF5I GGATG 3 cut(s) 127, 233, 511
BstFNI CGCG 1 cut(s) 473
BstH2I RGCGCY 2 cut(s) 349, 874
BstHHI GCGC 2 cut(s) 348, 873
BstKTI GATC 2 cut(s) 622, 853
BstMBI GATC 2 cut(s) 619, 850
BstMWI GCNNNNNNNGC 4 cut(s) 51, 257, 467, 470
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 829
BstSCI CCNGG 3 cut(s) 56, 611, 1011
BstSFI CTRYAG 3 cut(s) 535, 593, 662
BstUI CGCG 1 cut(s) 473
BstV1I GCAGC 1 cut(s) 448
BstX2I RGATCY 1 cut(s) 850
BstYI RGATCY 1 cut(s) 850
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 3 cut(s) 470, 654, 739
BtgI CCRYGG 2 cut(s) 97, 117
BtsCI GGATG 3 cut(s) 127, 233, 511
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 2 cut(s) 277, 725
BveI ACCTGC 1 cut(s) 388
CaiI CAGNNNCTG 2 cut(s) 38, 584
CfoI GCGC 2 cut(s) 348, 873
Cfr10I RCCGGY 1 cut(s) 969
Cfr13I GGNCC 1 cut(s) 653
Csp6I GTAC 4 cut(s) 355, 404, 684, 758
CspAI ACCGGT 1 cut(s) 969
CviAII CATG 3 cut(s) 245, 360, 826
CviQI GTAC 4 cut(s) 355, 404, 684, 758
DdeI CTNAG 7 cut(s) 162, 285, 546, 585, 741, 866, 937
DinI GGCGCC 1 cut(s) 872
DpnI GATC 2 cut(s) 621, 852
DpnII GATC 2 cut(s) 619, 850
DriI GACNNNNNGTC 1 cut(s) 633
EaeI YGGCCR 1 cut(s) 468
Eam1105I GACNNNNNGTC 1 cut(s) 633
Ecl136II GAGCTC 1 cut(s) 936
Eco147I AGGCCT 1 cut(s) 739
Eco24I GRGCYC 1 cut(s) 938
Eco47III AGCGCT 1 cut(s) 347
Eco53kI GAGCTC 1 cut(s) 936
Eco57I CTGAAG 4 cut(s) 510, 594, 792, 828
Eco81I CCTNAGG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 936
EcoRII CCWGG 1 cut(s) 56
EcoT22I ATGCAT 1 cut(s) 827
EcoT38I GRGCYC 1 cut(s) 938
EgeI GGCGCC 1 cut(s) 872
EheI GGCGCC 1 cut(s) 872
FaeI CATG 3 cut(s) 248, 363, 829
FalI AAGNNNNNCTT 6 cut(s) 173, 205, 402, 434, 765, 797
FaqI GGGAC 3 cut(s) 315, 406, 814
FatI CATG 3 cut(s) 244, 359, 825
FauNDI CATATG 2 cut(s) 790, 821
FblI GTMKAC 1 cut(s) 668
Fnu4HI GCNGC 2 cut(s) 462, 471
FokI GGATG 3 cut(s) 134, 240, 518
FriOI GRGCYC 1 cut(s) 938
Fsp4HI GCNGC 2 cut(s) 462, 471
FspBI CTAG 2 cut(s) 854, 956
GlaI GCGC 2 cut(s) 347, 872
GluI GCNGC 2 cut(s) 462, 471
GsuI CTGGAG 1 cut(s) 60
HaeII RGCGCY 2 cut(s) 349, 874
HaeIII GGCC 3 cut(s) 470, 654, 739
HapII CCGG 5 cut(s) 267, 612, 800, 970, 1012
HhaI GCGC 2 cut(s) 348, 873
Hin1I GRCGYC 1 cut(s) 871
Hin1II CATG 3 cut(s) 248, 363, 829
Hin6I GCGC 2 cut(s) 346, 871
HinP1I GCGC 2 cut(s) 346, 871
HincII GTYRAC 2 cut(s) 669, 754
HindII GTYRAC 2 cut(s) 669, 754
HindIII AAGCTT 3 cut(s) 68, 146, 701
HinfI GANTC 6 cut(s) 203, 475, 509, 581, 722, 995
HpaII CCGG 5 cut(s) 267, 612, 800, 970, 1012
HphI GGTGA 1 cut(s) 943
Hpy166II GTNNAC 4 cut(s) 97, 357, 669, 754
Hpy188I TCNGA 8 cut(s) 12, 110, 163, 523, 586, 744, 897, 940
Hpy188III TCNNGA 1 cut(s) 284
Hpy8I GTNNAC 4 cut(s) 97, 357, 669, 754
HpyAV CCTTC 9 cut(s) 12, 218, 218, 485, 697, 703, 707, 712, 803
HpyCH4III ACNGT 3 cut(s) 106, 631, 673
HpyCH4IV ACGT 3 cut(s) 310, 482, 756
HpyCH4V TGCA 8 cut(s) 90, 537, 595, 602, 664, 680, 825, 949
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 257, 467, 470
HpyF3I CTNAG 7 cut(s) 162, 285, 546, 585, 741, 866, 937
HpySE526I ACGT 3 cut(s) 310, 482, 756
Hsp92I GRCGYC 1 cut(s) 871
Hsp92II CATG 3 cut(s) 248, 363, 829
HspAI GCGC 2 cut(s) 346, 871
KasI GGCGCC 1 cut(s) 870
Kzo9I GATC 2 cut(s) 619, 850
LmnI GCTCC 2 cut(s) 59, 265
Lsp1109I GCAGC 1 cut(s) 448
LweI GCATC 2 cut(s) 167, 422
MaeI CTAG 2 cut(s) 854, 956
MaeII ACGT 3 cut(s) 310, 482, 756
MaeIII GTNAC 4 cut(s) 28, 625, 723, 965
MalI GATC 2 cut(s) 621, 852
MboI GATC 2 cut(s) 619, 850
MboII GAAGA 5 cut(s) 65, 685, 757, 825, 910
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 850
MhlI GDGCHC 1 cut(s) 938
MluCI AATT 6 cut(s) 219, 372, 657, 883, 889, 944
Mly113I GGCGCC 1 cut(s) 871
MlyI GAGTC 4 cut(s) 197, 518, 731, 989
MmeI TCCRAC 2 cut(s) 483, 941
MnlI CCTC 9 cut(s) 35, 274, 280, 463, 580, 750, 796, 861, 1009
Mph1103I ATGCAT 1 cut(s) 827
MroXI GAANNNNTTC 2 cut(s) 176, 777
MseI TTAA 6 cut(s) 66, 371, 387, 399, 408, 840
MslI CAYNNNNRTG 1 cut(s) 824
MspI CCGG 5 cut(s) 267, 612, 800, 970, 1012
MspR9I CCNGG 3 cut(s) 58, 613, 1013
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 58
MvnI CGCG 1 cut(s) 473
MwoI GCNNNNNNNGC 4 cut(s) 51, 257, 467, 470
NarI GGCGCC 1 cut(s) 871
NciI CCSGG 2 cut(s) 613, 1013
NdeI CATATG 2 cut(s) 790, 821
NdeII GATC 2 cut(s) 619, 850
NlaIII CATG 3 cut(s) 248, 363, 829
NlaIV GGNNCC 4 cut(s) 55, 103, 305, 872
NmeAIII GCCGAG 1 cut(s) 194
NmuCI GTSAC 2 cut(s) 625, 723
NsiI ATGCAT 1 cut(s) 827
NspI RCATGY 1 cut(s) 829
PceI AGGCCT 1 cut(s) 739
PdmI GAANNNNTTC 2 cut(s) 176, 777
PfeI GAWTC 2 cut(s) 475, 581
Pfl23II CGTACG 1 cut(s) 757
PinAI ACCGGT 1 cut(s) 969
PkrI GCNGC 2 cut(s) 463, 472
PleI GAGTC 4 cut(s) 197, 517, 730, 989
PluTI GGCGCC 1 cut(s) 874
PpsI GAGTC 4 cut(s) 197, 517, 730, 989
Psp124BI GAGCTC 1 cut(s) 938
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspLI CGTACG 1 cut(s) 757
PspN4I GGNNCC 4 cut(s) 55, 103, 305, 872
PspPI GGNCC 1 cut(s) 653
PstI CTGCAG 3 cut(s) 539, 597, 666
PstNI CAGNNNCTG 2 cut(s) 38, 584
PsuI RGATCY 1 cut(s) 850
RsaI GTAC 4 cut(s) 356, 405, 685, 759
RsaNI GTAC 4 cut(s) 355, 404, 684, 758
RseI CAYNNNNRTG 1 cut(s) 824
SacI GAGCTC 1 cut(s) 938
SalI GTCGAC 1 cut(s) 667
SaqAI TTAA 6 cut(s) 66, 371, 387, 399, 408, 840
SatI GCNGC 2 cut(s) 462, 471
Sau3AI GATC 2 cut(s) 619, 850
Sau96I GGNCC 1 cut(s) 653
SchI GAGTC 4 cut(s) 197, 518, 731, 989
ScrFI CCNGG 3 cut(s) 58, 613, 1013
SduI GDGCHC 1 cut(s) 938
SfaNI GCATC 2 cut(s) 167, 422
SfcI CTRYAG 3 cut(s) 535, 593, 662
SfoI GGCGCC 1 cut(s) 872
SmiMI CAYNNNNRTG 1 cut(s) 824
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 6 cut(s) 219, 372, 657, 883, 889, 944
SseBI AGGCCT 1 cut(s) 739
SsiI CCGC 1 cut(s) 471
SspDI GGCGCC 1 cut(s) 870
SspMI CTAG 2 cut(s) 854, 956
SstI GAGCTC 1 cut(s) 938
StuI AGGCCT 1 cut(s) 739
StyD4I CCNGG 3 cut(s) 56, 611, 1011
TaaI ACNGT 3 cut(s) 106, 631, 673
TaiI ACGT 3 cut(s) 313, 485, 759
TaqI TCGA 1 cut(s) 668
TasI AATT 6 cut(s) 219, 372, 657, 883, 889, 944
TatI WGTACW 2 cut(s) 354, 403
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 2 cut(s) 475, 581
Tru1I TTAA 6 cut(s) 66, 371, 387, 399, 408, 840
Tru9I TTAA 6 cut(s) 66, 371, 387, 399, 408, 840
TscAI CASTG 2 cut(s) 277, 732
TseFI GTSAC 2 cut(s) 625, 723
TseI GCWGC 1 cut(s) 461
Tsp45I GTSAC 2 cut(s) 625, 723
TspDTI ATGAA 3 cut(s) 233, 679, 685
TspGWI ACGGA 2 cut(s) 114, 134
TspRI CASTG 2 cut(s) 277, 732
XceI RCATGY 1 cut(s) 829
XmiI GTMKAC 1 cut(s) 668
XmnI GAANNNNTTC 2 cut(s) 176, 777
XspI CTAG 2 cut(s) 854, 956
Zsp2I ATGCAT 1 cut(s) 827
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.