RchiOBHm_Chr7g0216761

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0002886
Physical Location & Seq
Reverse (-)
34424877 .. 34428117
3241 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ19396

Sequence Viewer

Length: 960 bp
ATGGCAGTTCAGAATGGAAGGTTCTGTGTTACAGGGGCTGGAGGCTTCGTTGGCTCCTGGGTCGTTAAGCTTCTTCTTTCCAAGAACTGCATAGTCCACGGAACCGTCAGAGAACCCACGGATGACAAGAACTCTCACTTGAGAAAGCTTGACAAGGCATCTGAGAACCTGAAACTTTTCAAGGCGGACTTATTGGATTATGACTCCCTTTGCTTGGCAATTGATGGATGTGTTGGAGTATTTCATGTTGCCAGTCCTGTTCCCTCCGGGAGTGTACCAAATCCTGAGGTAGAGTTAATTCAACCTGCTGTTAAGGGGACACTTAATGTACTTAAAGCATCTCTTGAAGCAGAAGTCAAGCGAGTTGTTTATGTGTCCTCTGTTGCTGCTGTGACCATGAATCCTACGTGGGCTGAAGGTCAAGTATTGGATGAGTCTTGTTGGTCAGATAAGGAATACTGCAGAAATACTGAGAACTGGTATTGCCTTTCAAAAACCGAAGCAGAATCTGAGGCTCTGCAGTTTGCAAAAACAACCGGGTTTGATCTTGTGACTGTTTGTCCTACACTTATACTTGGCCCAATTCTGCAGTTGACTGTAAATGCAAGTACCTTGGTCCTCATCAAGCTTTTGAAGGAAGGAAAGGAGTCACTGGAAAACAGGCCCCGGAAGATAGTTGATGTACGGGATTTTGCTGAAGCACTTCTTATGGCATATGAGAAGCCGGAGGCTGAAGGAAGATACATATGCATGTCCCACAATGTTAAGAATAGGGATCTAGTTGAGAAGCTGAGGCGCCTTTATCCGAATTACAATTATCCGAAGAACTTTGTTGAAGTAGGCGAAGACAGACAGTTGAGCTCAGAGAAACTGCAAAGGCTAGGTTGGAGTTACCGGTCATTGGATGATACTCTCATTGACTCTGTTGAAAGCTACCGGGAGGCTGGACTATTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

319

Amino Acids

35.6

Weight (kDa)

5.48

Isoelectric Point (pI)

29.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 132 2.4e-07 NmrA-like family
Epimerase PF01370 9 - 245 6.2e-25 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 132 6.6e-08 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 203 1.8e-20 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 263 1.9e-14 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 193 4.7e-07 Male sterility protein
NAD_binding_10 PF13460 12 - 133 1.4e-10 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 795
AciI CCGC 1 cut(s) 185
AclWI GGATC 1 cut(s) 783
AcuI CTGAAG 3 cut(s) 435, 717, 753
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 4 cut(s) 276, 330, 610, 684
AfiI CCNNNNNNNGG 2 cut(s) 214, 901
AgeI ACCGGT 1 cut(s) 894
AgsI TTSAA 7 cut(s) 181, 302, 347, 492, 634, 836, 929
AhdI GACNNNNNGTC 1 cut(s) 558
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 6 cut(s) 70, 148, 628, 790, 861, 933
AluI AGCT 6 cut(s) 70, 148, 628, 790, 861, 933
Alw21I GWGCWC 1 cut(s) 863
AlwI GGATC 1 cut(s) 783
AlwNI CAGNNNCTG 2 cut(s) 38, 509
AoxI GGCC 2 cut(s) 577, 662
ApeKI GCWGC 1 cut(s) 386
AsiGI ACCGGT 1 cut(s) 894
Asp700I GAANNNNTTC 2 cut(s) 176, 702
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 3 cut(s) 578, 616, 663
AsuC2I CCSGG 4 cut(s) 268, 538, 667, 938
AvaII GGWCC 1 cut(s) 616
AxyI CCTNAGG 1 cut(s) 285
BanI GGYRCC 1 cut(s) 795
BanII GRGCYC 1 cut(s) 863
BbsI GAAGAC 1 cut(s) 852
Bbv12I GWGCWC 1 cut(s) 863
BbvCI CCTCAGC 1 cut(s) 791
BbvI GCAGC 1 cut(s) 373
BccI CCATC 1 cut(s) 218
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 4 cut(s) 268, 538, 667, 938
BfaI CTAG 2 cut(s) 779, 881
BfmI CTRYAG 3 cut(s) 460, 518, 587
BfoI RGCGCY 1 cut(s) 799
BfuAI ACCTGC 1 cut(s) 313
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
Bme1390I CCNGG 5 cut(s) 58, 268, 538, 667, 938
Bme18I GGWCC 1 cut(s) 616
BmeRI GACNNNNNGTC 1 cut(s) 558
BmgT120I GGNCC 3 cut(s) 578, 616, 663
BmiI GGNNCC 4 cut(s) 55, 103, 665, 797
BmrFI CCNGG 5 cut(s) 58, 268, 538, 667, 938
BmsI GCATC 2 cut(s) 167, 347
BpiI GAAGAC 1 cut(s) 852
BpmI CTGGAG 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 791
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 4 cut(s) 268, 538, 667, 938
BsaAI YACGTR 1 cut(s) 408
BsaHI GRCGYC 1 cut(s) 796
BsaJI CCNNGG 5 cut(s) 57, 97, 117, 612, 665
BsaWI WCCGGW 1 cut(s) 894
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 901
Bse118I RCCGGY 1 cut(s) 894
Bse1I ACTGG 3 cut(s) 252, 482, 657
Bse21I CCTNAGG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 5 cut(s) 57, 97, 117, 612, 665
BseGI GGATG 4 cut(s) 127, 233, 436, 910
BseLI CCNNNNNNNGG 2 cut(s) 214, 901
BseMII CTCAG 6 cut(s) 153, 276, 462, 501, 782, 876
BseNI ACTGG 3 cut(s) 252, 482, 657
BseXI GCAGC 1 cut(s) 373
BshFI GGCC 2 cut(s) 579, 664
BshNI GGYRCC 1 cut(s) 795
BshTI ACCGGT 1 cut(s) 894
BsiHKAI GWGCWC 1 cut(s) 863
BsiSI CCGG 6 cut(s) 267, 537, 667, 725, 895, 937
BslFI GGGAC 2 cut(s) 331, 739
BslI CCNNNNNNNGG 2 cut(s) 214, 901
BsmFI GGGAC 2 cut(s) 331, 739
BsnI GGCC 2 cut(s) 579, 664
Bsp1286I GDGCHC 1 cut(s) 863
Bsp143I GATC 2 cut(s) 544, 775
BspACI CCGC 1 cut(s) 185
BspANI GGCC 2 cut(s) 579, 664
BspCNI CTCAG 6 cut(s) 154, 277, 463, 502, 783, 875
BspLI GGNNCC 4 cut(s) 55, 103, 665, 797
BspMAI CTGCAG 3 cut(s) 464, 522, 591
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 1 cut(s) 783
BspT107I GGYRCC 1 cut(s) 795
BsrFI RCCGGY 1 cut(s) 894
BsrI ACTGG 3 cut(s) 252, 482, 657
BssAI RCCGGY 1 cut(s) 894
BssECI CCNNGG 5 cut(s) 57, 97, 117, 612, 665
BssMI GATC 2 cut(s) 544, 775
BssNI GRCGYC 1 cut(s) 796
BssT1I CCWWGG 1 cut(s) 612
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 4 cut(s) 106, 556, 598, 855
BstACI GRCGYC 1 cut(s) 796
BstBAI YACGTR 1 cut(s) 408
BstDEI CTNAG 6 cut(s) 162, 285, 471, 510, 791, 862
BstDSI CCRYGG 2 cut(s) 97, 117
BstF5I GGATG 4 cut(s) 127, 233, 436, 910
BstH2I RGCGCY 1 cut(s) 799
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 2 cut(s) 547, 778
BstMBI GATC 2 cut(s) 544, 775
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 754
BstSCI CCNGG 5 cut(s) 56, 266, 536, 665, 936
BstSFI CTRYAG 3 cut(s) 460, 518, 587
BstV1I GCAGC 1 cut(s) 373
BstV2I GAAGAC 1 cut(s) 852
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 2 cut(s) 579, 664
BtgI CCRYGG 2 cut(s) 97, 117
BtsCI GGATG 4 cut(s) 127, 233, 436, 910
BtsIMutI CAGTG 1 cut(s) 650
BveI ACCTGC 1 cut(s) 313
CaiI CAGNNNCTG 2 cut(s) 38, 509
CfoI GCGC 1 cut(s) 798
Cfr10I RCCGGY 1 cut(s) 894
Cfr13I GGNCC 3 cut(s) 578, 616, 663
Csp6I GTAC 4 cut(s) 275, 329, 609, 683
CspAI ACCGGT 1 cut(s) 894
CviAII CATG 3 cut(s) 245, 397, 751
CviQI GTAC 4 cut(s) 275, 329, 609, 683
DdeI CTNAG 6 cut(s) 162, 285, 471, 510, 791, 862
DinI GGCGCC 1 cut(s) 797
DpnI GATC 2 cut(s) 546, 777
DpnII GATC 2 cut(s) 544, 775
DriI GACNNNNNGTC 1 cut(s) 558
Eam1105I GACNNNNNGTC 1 cut(s) 558
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 861
Eco130I CCWWGG 1 cut(s) 612
Eco24I GRGCYC 1 cut(s) 863
Eco47I GGWCC 1 cut(s) 616
Eco53kI GAGCTC 1 cut(s) 861
Eco57I CTGAAG 3 cut(s) 435, 717, 753
Eco81I CCTNAGG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 861
EcoO109I RGGNCCY 1 cut(s) 663
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 612
EcoT22I ATGCAT 1 cut(s) 752
EcoT38I GRGCYC 1 cut(s) 863
EgeI GGCGCC 1 cut(s) 797
EheI GGCGCC 1 cut(s) 797
ErhI CCWWGG 1 cut(s) 612
FaeI CATG 3 cut(s) 248, 400, 754
FalI AAGNNNNNCTT 6 cut(s) 173, 205, 327, 359, 690, 722
FaqI GGGAC 2 cut(s) 331, 739
FatI CATG 3 cut(s) 244, 396, 750
FauNDI CATATG 2 cut(s) 715, 746
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 4 cut(s) 134, 240, 443, 917
FriOI GRGCYC 1 cut(s) 863
Fsp4HI GCNGC 1 cut(s) 387
FspBI CTAG 2 cut(s) 779, 881
GlaI GCGC 1 cut(s) 797
GluI GCNGC 1 cut(s) 387
GsuI CTGGAG 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 799
HaeIII GGCC 2 cut(s) 579, 664
HapII CCGG 6 cut(s) 267, 537, 667, 725, 895, 937
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 3 cut(s) 248, 400, 754
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HincII GTYRAC 1 cut(s) 594
HindII GTYRAC 1 cut(s) 594
HindIII AAGCTT 3 cut(s) 68, 146, 626
HinfI GANTC 6 cut(s) 203, 400, 434, 506, 647, 920
HpaII CCGG 6 cut(s) 267, 537, 667, 725, 895, 937
Hpy166II GTNNAC 3 cut(s) 97, 275, 594
Hpy188I TCNGA 8 cut(s) 12, 110, 163, 448, 511, 807, 822, 865
Hpy188III TCNNGA 2 cut(s) 284, 344
Hpy8I GTNNAC 3 cut(s) 97, 275, 594
HpyAV CCTTC 5 cut(s) 12, 410, 628, 632, 728
HpyCH4III ACNGT 4 cut(s) 106, 556, 598, 855
HpyCH4IV ACGT 1 cut(s) 407
HpyCH4V TGCA 8 cut(s) 90, 462, 520, 527, 589, 605, 750, 874
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 6 cut(s) 162, 285, 471, 510, 791, 862
HpySE526I ACGT 1 cut(s) 407
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 3 cut(s) 248, 400, 754
HspAI GCGC 1 cut(s) 796
KasI GGCGCC 1 cut(s) 795
Kzo9I GATC 2 cut(s) 544, 775
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 2 cut(s) 167, 347
MaeI CTAG 2 cut(s) 779, 881
MaeII ACGT 1 cut(s) 407
MaeIII GTNAC 5 cut(s) 28, 391, 550, 648, 890
MalI GATC 2 cut(s) 546, 777
MboI GATC 2 cut(s) 544, 775
MboII GAAGA 5 cut(s) 65, 682, 750, 835, 857
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 1 cut(s) 863
MluCI AATT 5 cut(s) 219, 297, 582, 808, 814
Mly113I GGCGCC 1 cut(s) 796
MlyI GAGTC 4 cut(s) 197, 443, 656, 914
MmeI TCCRAC 2 cut(s) 214, 866
MnlI CCTC 9 cut(s) 35, 274, 280, 388, 505, 629, 721, 786, 934
Mph1103I ATGCAT 1 cut(s) 752
MroXI GAANNNNTTC 2 cut(s) 176, 702
MseI TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
MslI CAYNNNNRTG 1 cut(s) 749
MspI CCGG 6 cut(s) 267, 537, 667, 725, 895, 937
MspR9I CCNGG 5 cut(s) 58, 268, 538, 667, 938
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 1 cut(s) 51
NarI GGCGCC 1 cut(s) 796
NciI CCSGG 4 cut(s) 268, 538, 667, 938
NdeI CATATG 2 cut(s) 715, 746
NdeII GATC 2 cut(s) 544, 775
NlaIII CATG 3 cut(s) 248, 400, 754
NlaIV GGNNCC 4 cut(s) 55, 103, 665, 797
NmuCI GTSAC 3 cut(s) 391, 550, 648
NsiI ATGCAT 1 cut(s) 752
NspI RCATGY 1 cut(s) 754
PdmI GAANNNNTTC 2 cut(s) 176, 702
PfeI GAWTC 2 cut(s) 400, 506
PfoI TCCNGGA 1 cut(s) 266
PinAI ACCGGT 1 cut(s) 894
PkrI GCNGC 1 cut(s) 388
PleI GAGTC 4 cut(s) 197, 442, 655, 914
PluTI GGCGCC 1 cut(s) 799
PpsI GAGTC 4 cut(s) 197, 442, 655, 914
Ppu21I YACGTR 1 cut(s) 408
Psp124BI GAGCTC 1 cut(s) 863
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 4 cut(s) 55, 103, 665, 797
PspPI GGNCC 3 cut(s) 578, 616, 663
PstI CTGCAG 3 cut(s) 464, 522, 591
PstNI CAGNNNCTG 2 cut(s) 38, 509
PsuI RGATCY 1 cut(s) 775
RsaI GTAC 4 cut(s) 276, 330, 610, 684
RsaNI GTAC 4 cut(s) 275, 329, 609, 683
RseI CAYNNNNRTG 1 cut(s) 749
SacI GAGCTC 1 cut(s) 863
SaqAI TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 2 cut(s) 544, 775
Sau96I GGNCC 3 cut(s) 578, 616, 663
SchI GAGTC 4 cut(s) 197, 443, 656, 914
ScrFI CCNGG 5 cut(s) 58, 268, 538, 667, 938
SduI GDGCHC 1 cut(s) 863
SfaNI GCATC 2 cut(s) 167, 347
SfcI CTRYAG 3 cut(s) 460, 518, 587
SfoI GGCGCC 1 cut(s) 797
SinI GGWCC 1 cut(s) 616
SmiMI CAYNNNNRTG 1 cut(s) 749
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 5 cut(s) 219, 297, 582, 808, 814
SsiI CCGC 1 cut(s) 185
SspDI GGCGCC 1 cut(s) 795
SspMI CTAG 2 cut(s) 779, 881
SstI GAGCTC 1 cut(s) 863
StyD4I CCNGG 5 cut(s) 56, 266, 536, 665, 936
StyI CCWWGG 1 cut(s) 612
TaaI ACNGT 4 cut(s) 106, 556, 598, 855
TaiI ACGT 1 cut(s) 410
TasI AATT 5 cut(s) 219, 297, 582, 808, 814
TatI WGTACW 1 cut(s) 328
TfiI GAWTC 2 cut(s) 400, 506
Tru1I TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
Tru9I TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
TscAI CASTG 1 cut(s) 657
TseFI GTSAC 3 cut(s) 391, 550, 648
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 3 cut(s) 391, 550, 648
TspDTI ATGAA 2 cut(s) 233, 413
TspGWI ACGGA 2 cut(s) 114, 134
TspRI CASTG 1 cut(s) 657
VpaK11BI GGWCC 1 cut(s) 616
XceI RCATGY 1 cut(s) 754
XmnI GAANNNNTTC 2 cut(s) 176, 702
XspI CTAG 2 cut(s) 779, 881
Zsp2I ATGCAT 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.