FvH4_5g26020

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002886
Physical Location & Seq
Forward (+)
17522094 .. 17525671
3578 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g26020.t2

Sequence Viewer

Length: 960 bp
ATGGCACTTCAGAACGGAAGGTTTTGTGTTACAGGTGCTGGAGGCTTCGTTGGCTCCTGGGTCGTTAAGCTTCTTCTCTCCAAGAACTGCATAGTCCACGGAACCATCAGAGAACCCACGGATGCAAAGTACTCTCATTTGAAAAAACTTGACAAGGCATCTGAGAACCTGAAACTGTTCAAGGCGGACTTATTGAATTATGACTCCCTTCGCACGGCAATTGAAGGATGTGATGGAGTGTTTCATGTTGCCAGTCCAGTTCCCCCCGGCAGTGTGCCGAATCCTGAGGCAAAGATAATTGAACCTGCTGTTAAGGGGACACTTAATGTACTTAAAGCATCTCTTGAAGCAAAAGTCAAGCGCGTTGTTTTTGTGTCTTCTGTTGCTGCTGTCTCTGTGAATCCTACATGGGCAGAAGGTCAAGTGTTAGATGAGACTTGTTGGTCTGATAAGGAATACTGCAGAAATACTGAGAACTGGTATTGCCTCTCAAAAACTGAAGCAGAATATGAGGCTCTGCAGTTTGCAAATACAACAGGGATTGATCTTGTGACTGTTTGTCCTACACTTATATTTGGCCCAATTCTGCAGTCGACTGTAAATGCAAGTACCTTGATCCTCATCAAGCTTTTGAAGGAAGGGAAGGAGTCACTGGAAAACAGGCCCCGGAAGATAGTTGACGTGCGGGATTTAGCTGAAGCACTTCTTATGGCATATGAGAAGCCGAAGGCTGAAGGAAGATACATATGCATGTCCCATAGCGTTAAGAACAGGGATCTAGTTGAGAAGCTGAGGCGCCTTTATCCTAATTACAATTATCCTAAGAACTTTGTTGAAGTAGGTGAAGAGAAACAGGTCACCTCAGAGAAACTGCAAAGGCTTGGGTGGGGTTACCGGCCATTGGACGATACTCTCATTGACTCTGTTGAAAGCTACCGAGAAGCTGGACTATTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

320

Amino Acids

35.56

Weight (kDa)

6.91

Isoelectric Point (pI)

28.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 132 5e-09 NmrA-like family
KR PF08659 7 - 136 4.6e-06 KR domain
Epimerase PF01370 9 - 245 1.3e-28 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 136 3.2e-10 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 203 8.6e-22 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 271 1.1e-17 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 197 1.8e-09 Male sterility protein
NAD_binding_10 PF13460 12 - 132 1.4e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 442
Acc36I ACCTGC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 795
AccI GTMKAC 1 cut(s) 593
AccII CGCG 1 cut(s) 363
AciI CCGC 2 cut(s) 185, 685
AclWI GGATC 2 cut(s) 610, 783
AcoI YGGCCR 1 cut(s) 896
AcuI CTGAAG 3 cut(s) 519, 717, 753
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 3 cut(s) 131, 330, 610
AfiI CCNNNNNNNGG 2 cut(s) 214, 901
AgsI TTSAA 9 cut(s) 142, 181, 196, 224, 302, 347, 634, 836, 929
AhdI GACNNNNNGTC 1 cut(s) 558
AjiI CACGTC 1 cut(s) 682
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 6 cut(s) 70, 628, 695, 790, 933, 944
AluI AGCT 6 cut(s) 70, 628, 695, 790, 933, 944
Alw26I GTCTC 2 cut(s) 397, 428
AlwI GGATC 2 cut(s) 610, 783
AlwNI CAGNNNCTG 1 cut(s) 38
AoxI GGCC 3 cut(s) 577, 662, 896
ApeKI GCWGC 1 cut(s) 386
Asp700I GAANNNNTTC 2 cut(s) 176, 702
AspLEI GCGC 2 cut(s) 363, 798
AspS9I GGNCC 2 cut(s) 578, 663
AsuC2I CCSGG 2 cut(s) 267, 667
AsuHPI GGTGA 2 cut(s) 850, 854
AxyI CCTNAGG 1 cut(s) 285
BanI GGYRCC 1 cut(s) 795
BbsI GAAGAC 1 cut(s) 369
BbvCI CCTCAGC 1 cut(s) 791
BbvI GCAGC 1 cut(s) 373
BccI CCATC 2 cut(s) 113, 227
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 2 cut(s) 267, 667
BcoDI GTCTC 2 cut(s) 397, 428
BfaI CTAG 1 cut(s) 779
BfmI CTRYAG 3 cut(s) 460, 518, 587
BfoI RGCGCY 1 cut(s) 799
BfuAI ACCTGC 1 cut(s) 313
BisI GCNGC 1 cut(s) 387
BlsI GCNGC 1 cut(s) 388
BmcAI AGTACT 1 cut(s) 131
Bme1390I CCNGG 3 cut(s) 58, 267, 667
BmeRI GACNNNNNGTC 1 cut(s) 558
BmgBI CACGTC 1 cut(s) 682
BmgT120I GGNCC 2 cut(s) 578, 663
BmiI GGNNCC 4 cut(s) 55, 103, 665, 797
BmrFI CCNGG 3 cut(s) 58, 267, 667
BmsI GCATC 3 cut(s) 112, 167, 347
BpiI GAAGAC 1 cut(s) 369
BpmI CTGGAG 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 791
BpuMI CCSGG 2 cut(s) 267, 667
BsaBI GATNNNNATC 1 cut(s) 620
BsaHI GRCGYC 1 cut(s) 796
BsaJI CCNNGG 5 cut(s) 57, 97, 117, 265, 665
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 901
Bse118I RCCGGY 1 cut(s) 894
Bse1I ACTGG 4 cut(s) 252, 257, 482, 657
Bse21I CCTNAGG 1 cut(s) 285
Bse8I GATNNNNATC 1 cut(s) 620
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 5 cut(s) 57, 97, 117, 265, 665
BseGI GGATG 2 cut(s) 127, 233
BseJI GATNNNNATC 1 cut(s) 620
BseLI CCNNNNNNNGG 2 cut(s) 214, 901
BseMII CTCAG 5 cut(s) 153, 276, 462, 782, 876
BseNI ACTGG 4 cut(s) 252, 257, 482, 657
BseXI GCAGC 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 363
BshFI GGCC 3 cut(s) 579, 664, 898
BshNI GGYRCC 1 cut(s) 795
BsiSI CCGG 3 cut(s) 267, 667, 895
BslFI GGGAC 2 cut(s) 331, 739
BslI CCNNNNNNNGG 2 cut(s) 214, 901
BsmAI GTCTC 2 cut(s) 397, 428
BsmFI GGGAC 2 cut(s) 331, 739
BsnI GGCC 3 cut(s) 579, 664, 898
Bsp143I GATC 3 cut(s) 544, 615, 775
BspACI CCGC 2 cut(s) 185, 685
BspANI GGCC 3 cut(s) 579, 664, 898
BspCNI CTCAG 5 cut(s) 154, 277, 463, 783, 875
BspFNI CGCG 1 cut(s) 363
BspLI GGNNCC 4 cut(s) 55, 103, 665, 797
BspMAI CTGCAG 3 cut(s) 464, 522, 591
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 2 cut(s) 610, 783
BspT107I GGYRCC 1 cut(s) 795
BsrFI RCCGGY 1 cut(s) 894
BsrI ACTGG 4 cut(s) 252, 257, 482, 657
BssAI RCCGGY 1 cut(s) 894
BssECI CCNNGG 5 cut(s) 57, 97, 117, 265, 665
BssMI GATC 3 cut(s) 544, 615, 775
BssNI GRCGYC 1 cut(s) 796
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 3 cut(s) 177, 556, 598
Bst6I CTCTTC 1 cut(s) 840
BstACI GRCGYC 1 cut(s) 796
BstDEI CTNAG 6 cut(s) 162, 285, 471, 791, 822, 862
BstDSI CCRYGG 2 cut(s) 97, 117
BstEII GGTNACC 2 cut(s) 856, 890
BstF5I GGATG 2 cut(s) 127, 233
BstFNI CGCG 1 cut(s) 363
BstH2I RGCGCY 1 cut(s) 799
BstHHI GCGC 2 cut(s) 363, 798
BstKTI GATC 3 cut(s) 547, 618, 778
BstMAI GTCTC 2 cut(s) 397, 428
BstMBI GATC 3 cut(s) 544, 615, 775
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 754
BstPI GGTNACC 2 cut(s) 856, 890
BstSCI CCNGG 3 cut(s) 56, 265, 665
BstSFI CTRYAG 3 cut(s) 460, 518, 587
BstUI CGCG 1 cut(s) 363
BstV1I GCAGC 1 cut(s) 373
BstV2I GAAGAC 1 cut(s) 369
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 3 cut(s) 579, 664, 898
BtgI CCRYGG 2 cut(s) 97, 117
BtrI CACGTC 1 cut(s) 682
BtsCI GGATG 2 cut(s) 127, 233
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 2 cut(s) 277, 650
BveI ACCTGC 1 cut(s) 313
CaiI CAGNNNCTG 1 cut(s) 38
CfoI GCGC 2 cut(s) 363, 798
Cfr10I RCCGGY 1 cut(s) 894
Cfr13I GGNCC 2 cut(s) 578, 663
Csp6I GTAC 3 cut(s) 130, 329, 609
CviAII CATG 3 cut(s) 245, 408, 751
CviQI GTAC 3 cut(s) 130, 329, 609
DdeI CTNAG 6 cut(s) 162, 285, 471, 791, 822, 862
DinI GGCGCC 1 cut(s) 797
DpnI GATC 3 cut(s) 546, 617, 777
DpnII GATC 3 cut(s) 544, 615, 775
DrdI GACNNNNNNGTC 1 cut(s) 442
DriI GACNNNNNGTC 1 cut(s) 558
DseDI GACNNNNNNGTC 1 cut(s) 442
EaeI YGGCCR 1 cut(s) 896
Eam1104I CTCTTC 1 cut(s) 840
Eam1105I GACNNNNNGTC 1 cut(s) 558
EarI CTCTTC 1 cut(s) 840
EciI GGCGGA 1 cut(s) 200
Eco57I CTGAAG 3 cut(s) 519, 717, 753
Eco81I CCTNAGG 1 cut(s) 285
Eco91I GGTNACC 2 cut(s) 856, 890
EcoO109I RGGNCCY 1 cut(s) 663
EcoO65I GGTNACC 2 cut(s) 856, 890
EcoRII CCWGG 1 cut(s) 56
EcoT22I ATGCAT 1 cut(s) 752
EgeI GGCGCC 1 cut(s) 797
EheI GGCGCC 1 cut(s) 797
FaeI CATG 3 cut(s) 248, 411, 754
FalI AAGNNNNNCTT 6 cut(s) 173, 205, 327, 359, 690, 722
FaqI GGGAC 2 cut(s) 331, 739
FatI CATG 3 cut(s) 244, 407, 750
FauI CCCGC 1 cut(s) 678
FauNDI CATATG 2 cut(s) 715, 746
FblI GTMKAC 1 cut(s) 593
Fnu4HI GCNGC 1 cut(s) 387
FokI GGATG 2 cut(s) 134, 240
Fsp4HI GCNGC 1 cut(s) 387
FspBI CTAG 1 cut(s) 779
GlaI GCGC 2 cut(s) 362, 797
GluI GCNGC 1 cut(s) 387
GsuI CTGGAG 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 799
HaeIII GGCC 3 cut(s) 579, 664, 898
HapII CCGG 3 cut(s) 267, 667, 895
HhaI GCGC 2 cut(s) 363, 798
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 3 cut(s) 248, 411, 754
Hin6I GCGC 2 cut(s) 361, 796
HinP1I GCGC 2 cut(s) 361, 796
HincII GTYRAC 2 cut(s) 594, 679
HindII GTYRAC 2 cut(s) 594, 679
HindIII AAGCTT 2 cut(s) 68, 626
HinfI GANTC 5 cut(s) 203, 280, 400, 647, 920
HpaII CCGG 3 cut(s) 267, 667, 895
HphI GGTGA 2 cut(s) 850, 854
Hpy166II GTNNAC 3 cut(s) 97, 594, 679
Hpy188I TCNGA 5 cut(s) 12, 110, 163, 448, 865
Hpy188III TCNNGA 2 cut(s) 284, 344
Hpy8I GTNNAC 3 cut(s) 97, 594, 679
HpyAV CCTTC 9 cut(s) 12, 218, 218, 410, 628, 632, 637, 721, 728
HpyCH4III ACNGT 3 cut(s) 177, 556, 598
HpyCH4IV ACGT 1 cut(s) 681
HpyCH4V TGCA 9 cut(s) 90, 125, 462, 520, 527, 589, 605, 750, 874
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 6 cut(s) 162, 285, 471, 791, 822, 862
HpySE526I ACGT 1 cut(s) 681
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 3 cut(s) 248, 411, 754
HspAI GCGC 2 cut(s) 361, 796
KasI GGCGCC 1 cut(s) 795
Kzo9I GATC 3 cut(s) 544, 615, 775
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 3 cut(s) 112, 167, 347
MaeI CTAG 1 cut(s) 779
MaeII ACGT 1 cut(s) 681
MaeIII GTNAC 5 cut(s) 28, 550, 648, 856, 890
MalI GATC 3 cut(s) 546, 617, 777
MboI GATC 3 cut(s) 544, 615, 775
MboII GAAGA 5 cut(s) 65, 369, 682, 750, 857
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 775
MluCI AATT 6 cut(s) 196, 219, 297, 582, 808, 814
Mly113I GGCGCC 1 cut(s) 796
MlyI GAGTC 3 cut(s) 197, 656, 914
MnlI CCTC 7 cut(s) 35, 280, 497, 505, 629, 786, 871
Mph1103I ATGCAT 1 cut(s) 752
MroXI GAANNNNTTC 2 cut(s) 176, 702
MseI TTAA 5 cut(s) 66, 312, 324, 333, 765
MslI CAYNNNNRTG 1 cut(s) 749
MspI CCGG 3 cut(s) 267, 667, 895
MspR9I CCNGG 3 cut(s) 58, 267, 667
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 58
MvnI CGCG 1 cut(s) 363
MwoI GCNNNNNNNGC 1 cut(s) 51
NarI GGCGCC 1 cut(s) 796
NciI CCSGG 2 cut(s) 267, 667
NdeI CATATG 2 cut(s) 715, 746
NdeII GATC 3 cut(s) 544, 615, 775
NlaIII CATG 3 cut(s) 248, 411, 754
NlaIV GGNNCC 4 cut(s) 55, 103, 665, 797
NmuCI GTSAC 3 cut(s) 550, 648, 856
NsiI ATGCAT 1 cut(s) 752
NspI RCATGY 1 cut(s) 754
PdmI GAANNNNTTC 2 cut(s) 176, 702
PfeI GAWTC 2 cut(s) 280, 400
PkrI GCNGC 1 cut(s) 388
PleI GAGTC 3 cut(s) 197, 655, 914
PluTI GGCGCC 1 cut(s) 799
PpsI GAGTC 3 cut(s) 197, 655, 914
Psp6I CCWGG 1 cut(s) 56
PspEI GGTNACC 2 cut(s) 856, 890
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 4 cut(s) 55, 103, 665, 797
PspPI GGNCC 2 cut(s) 578, 663
PstI CTGCAG 3 cut(s) 464, 522, 591
PstNI CAGNNNCTG 1 cut(s) 38
PsuI RGATCY 1 cut(s) 775
RsaI GTAC 3 cut(s) 131, 330, 610
RsaNI GTAC 3 cut(s) 130, 329, 609
RseI CAYNNNNRTG 1 cut(s) 749
SalI GTCGAC 1 cut(s) 592
SaqAI TTAA 5 cut(s) 66, 312, 324, 333, 765
SatI GCNGC 1 cut(s) 387
Sau3AI GATC 3 cut(s) 544, 615, 775
Sau96I GGNCC 2 cut(s) 578, 663
ScaI AGTACT 1 cut(s) 131
SchI GAGTC 3 cut(s) 197, 656, 914
ScrFI CCNGG 3 cut(s) 58, 267, 667
SfaNI GCATC 3 cut(s) 112, 167, 347
SfcI CTRYAG 3 cut(s) 460, 518, 587
SfoI GGCGCC 1 cut(s) 797
SmiMI CAYNNNNRTG 1 cut(s) 749
Sse9I AATT 6 cut(s) 196, 219, 297, 582, 808, 814
SsiI CCGC 2 cut(s) 185, 685
SspDI GGCGCC 1 cut(s) 795
SspMI CTAG 1 cut(s) 779
StyD4I CCNGG 3 cut(s) 56, 265, 665
TaaI ACNGT 3 cut(s) 177, 556, 598
TaiI ACGT 1 cut(s) 684
TaqI TCGA 1 cut(s) 593
TasI AATT 6 cut(s) 196, 219, 297, 582, 808, 814
TatI WGTACW 2 cut(s) 129, 328
TfiI GAWTC 2 cut(s) 280, 400
Tru1I TTAA 5 cut(s) 66, 312, 324, 333, 765
Tru9I TTAA 5 cut(s) 66, 312, 324, 333, 765
TscAI CASTG 2 cut(s) 277, 657
TseFI GTSAC 3 cut(s) 550, 648, 856
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 3 cut(s) 550, 648, 856
TspDTI ATGAA 1 cut(s) 233
TspGWI ACGGA 3 cut(s) 30, 114, 134
TspRI CASTG 2 cut(s) 277, 657
XceI RCATGY 1 cut(s) 754
XmiI GTMKAC 1 cut(s) 593
XmnI GAANNNNTTC 2 cut(s) 176, 702
XspI CTAG 1 cut(s) 779
ZrmI AGTACT 1 cut(s) 131
Zsp2I ATGCAT 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.