Rroxscaffold_3G00242920

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002886
Physical Location & Seq
Forward (+)
35446074 .. 35449181
3108 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242920.1

Sequence Viewer

Length: 975 bp
ATGGCAGTTCAGAATGGCAGGTTCTGTGTTACAGGGGCTGGAGGCTTCGTTGGCTCCTGGGTCGTTAAGCTTCTTCTTTCCAAGAACTGCATAGTCCACGGAACCGTCAGAGAACCCATGGATGACAAGAACTCTCACTTGAGAAAGCTTGACAAAGCATCTGAGAACCTGAAACTATTCAAGGCGGACTTATTGGATTATGACTCCCTTTGCTTGGCAATTGATGGATGTGATGGAGTATTTCATGTTGCCAGTCCTGTTCCCTCCGGGAGTGTGCCAAATCCTGAGGCAAGTGTGTCTCTCGTAGAGTTAATTAAACCTGCTGTTAAGGGGACACTTAATGTACTTAAAGCATCTCTTGAAGCAAAAGTCAAGCGAGTTGTTTATGTGTCCTCTGTTGCTGCTGTGGCCATGAATCCTACGTGGGCTGAAGGTCAAGTATTGGATGAGTCTTGTTGGTCAGATAAGGAACACTGCAGAAATACTGAGAACTGGTATTGCCTTTCAAAAACTGAAGCAGAATCTGAGGCTCTGCAGTTTGCAAAAACAACCGGGATTGATCTTGTGACTGTTTGTCCTACACTTATACTTGGCCCAATTCTGCAGTCGACTGTAAATGCAAGTACCTTGGTCCTCATCAAGCTTTTGAAGGAAGGAAAAGAGTCACTGGAAAACAGGCCCCGGAAGATAGTTGACGTACGGGATTTAGCTGAAGCACTTCTTATGGCATATGAGAAGCCGGAGGCTGAAGGAAGATACATATGCATGTCCCACAATGTTAAGAATAGGGATCTAGTTGAGAAGCTGAGGCGCCTTTATCCGAATTACAATTATCCGAAGAACTTTGTTGAAGTTGGCGAAGACAGACAGGTGAGCTCAGAGAAACTGCAAAGGCTAGGTTGGAGTTACCGGTCATTGGACGATACTCTCATTGACTCTGTTGAAAGCTACCGGGAGGCTGGACTATTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

324

Amino Acids

35.94

Weight (kDa)

5.87

Isoelectric Point (pI)

29.5

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 137 1.1e-07 NmrA-like family
KR PF08659 7 - 140 8.5e-07 KR domain
Epimerase PF01370 9 - 250 1.8e-26 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 141 5.4e-09 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 208 1.4e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 269 6.9e-15 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 137 1.4e-11 NAD(P)H-binding
NAD_binding_4 PF07993 100 - 202 2.7e-06 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 9, 328
AccB1I GGYRCC 1 cut(s) 810
AccI GTMKAC 1 cut(s) 608
AciI CCGC 1 cut(s) 185
AclWI GGATC 1 cut(s) 798
AcoI YGGCCR 1 cut(s) 408
AcuI CTGAAG 4 cut(s) 450, 534, 732, 768
AcyI GRCGYC 1 cut(s) 811
AfaI GTAC 3 cut(s) 345, 625, 699
AfiI CCNNNNNNNGG 2 cut(s) 214, 916
AgeI ACCGGT 1 cut(s) 909
AgsI TTSAA 6 cut(s) 181, 362, 507, 649, 851, 944
AhdI GACNNNNNGTC 1 cut(s) 573
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 7 cut(s) 70, 148, 643, 710, 805, 876, 948
AluI AGCT 7 cut(s) 70, 148, 643, 710, 805, 876, 948
Alw21I GWGCWC 1 cut(s) 878
Alw26I GTCTC 1 cut(s) 303
AlwI GGATC 1 cut(s) 798
AlwNI CAGNNNCTG 3 cut(s) 24, 38, 524
AoxI GGCC 3 cut(s) 408, 592, 677
ApeKI GCWGC 1 cut(s) 401
AsiGI ACCGGT 1 cut(s) 909
Asp700I GAANNNNTTC 2 cut(s) 176, 717
AspLEI GCGC 1 cut(s) 813
AspS9I GGNCC 3 cut(s) 593, 631, 678
AsuC2I CCSGG 4 cut(s) 268, 553, 682, 953
AsuHPI GGTGA 1 cut(s) 883
AvaII GGWCC 1 cut(s) 631
AxyI CCTNAGG 1 cut(s) 285
BalI TGGCCA 1 cut(s) 410
BanI GGYRCC 1 cut(s) 810
BanII GRGCYC 1 cut(s) 878
BbsI GAAGAC 1 cut(s) 867
Bbv12I GWGCWC 1 cut(s) 878
BbvCI CCTCAGC 1 cut(s) 806
BbvI GCAGC 1 cut(s) 388
BccI CCATC 2 cut(s) 218, 227
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 4 cut(s) 268, 553, 682, 953
BcoDI GTCTC 1 cut(s) 303
BfaI CTAG 2 cut(s) 794, 896
BfmI CTRYAG 3 cut(s) 475, 533, 602
BfoI RGCGCY 1 cut(s) 814
BfuAI ACCTGC 2 cut(s) 9, 328
BisI GCNGC 1 cut(s) 402
BlsI GCNGC 1 cut(s) 403
Bme1390I CCNGG 5 cut(s) 58, 268, 553, 682, 953
Bme18I GGWCC 1 cut(s) 631
BmeRI GACNNNNNGTC 1 cut(s) 573
BmgT120I GGNCC 3 cut(s) 593, 631, 678
BmiI GGNNCC 4 cut(s) 55, 103, 680, 812
BmrFI CCNGG 5 cut(s) 58, 268, 553, 682, 953
BmsI GCATC 2 cut(s) 167, 362
BpiI GAAGAC 1 cut(s) 867
BpmI CTGGAG 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 806
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 4 cut(s) 268, 553, 682, 953
BsaAI YACGTR 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 811
BsaJI CCNNGG 5 cut(s) 57, 97, 117, 627, 680
BsaWI WCCGGW 1 cut(s) 909
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 916
Bse118I RCCGGY 1 cut(s) 909
Bse1I ACTGG 3 cut(s) 252, 497, 672
Bse21I CCTNAGG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 5 cut(s) 57, 97, 117, 627, 680
BseGI GGATG 3 cut(s) 127, 233, 451
BseLI CCNNNNNNNGG 2 cut(s) 214, 916
BseMII CTCAG 6 cut(s) 153, 276, 477, 516, 797, 891
BseNI ACTGG 3 cut(s) 252, 497, 672
BseXI GCAGC 1 cut(s) 388
BshFI GGCC 3 cut(s) 410, 594, 679
BshNI GGYRCC 1 cut(s) 810
BshTI ACCGGT 1 cut(s) 909
BsiHKAI GWGCWC 1 cut(s) 878
BsiSI CCGG 6 cut(s) 267, 552, 682, 740, 910, 952
BsiWI CGTACG 1 cut(s) 697
BslFI GGGAC 2 cut(s) 346, 754
BslI CCNNNNNNNGG 2 cut(s) 214, 916
BsmAI GTCTC 1 cut(s) 303
BsmFI GGGAC 2 cut(s) 346, 754
BsnI GGCC 3 cut(s) 410, 594, 679
Bsp1286I GDGCHC 1 cut(s) 878
Bsp143I GATC 2 cut(s) 559, 790
Bsp19I CCATGG 1 cut(s) 117
BspACI CCGC 1 cut(s) 185
BspANI GGCC 3 cut(s) 410, 594, 679
BspCNI CTCAG 6 cut(s) 154, 277, 478, 517, 798, 890
BspLI GGNNCC 4 cut(s) 55, 103, 680, 812
BspMAI CTGCAG 3 cut(s) 479, 537, 606
BspMI ACCTGC 2 cut(s) 9, 328
BspPI GGATC 1 cut(s) 798
BspT107I GGYRCC 1 cut(s) 810
BsrFI RCCGGY 1 cut(s) 909
BsrI ACTGG 3 cut(s) 252, 497, 672
BssAI RCCGGY 1 cut(s) 909
BssECI CCNNGG 5 cut(s) 57, 97, 117, 627, 680
BssMI GATC 2 cut(s) 559, 790
BssNI GRCGYC 1 cut(s) 811
BssT1I CCWWGG 2 cut(s) 117, 627
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 3 cut(s) 106, 571, 613
BstACI GRCGYC 1 cut(s) 811
BstBAI YACGTR 1 cut(s) 423
BstDEI CTNAG 6 cut(s) 162, 285, 486, 525, 806, 877
BstDSI CCRYGG 2 cut(s) 97, 117
BstF5I GGATG 3 cut(s) 127, 233, 451
BstH2I RGCGCY 1 cut(s) 814
BstHHI GCGC 1 cut(s) 813
BstKTI GATC 2 cut(s) 562, 793
BstMAI GTCTC 1 cut(s) 303
BstMBI GATC 2 cut(s) 559, 790
BstMWI GCNNNNNNNGC 2 cut(s) 51, 407
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 769
BstSCI CCNGG 5 cut(s) 56, 266, 551, 680, 951
BstSFI CTRYAG 3 cut(s) 475, 533, 602
BstV1I GCAGC 1 cut(s) 388
BstV2I GAAGAC 1 cut(s) 867
BstX2I RGATCY 1 cut(s) 790
BstYI RGATCY 1 cut(s) 790
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 3 cut(s) 410, 594, 679
BtgI CCRYGG 2 cut(s) 97, 117
BtsCI GGATG 3 cut(s) 127, 233, 451
BtsI GCAGTG 1 cut(s) 472
BtsIMutI CAGTG 2 cut(s) 472, 665
BveI ACCTGC 2 cut(s) 9, 328
CaiI CAGNNNCTG 3 cut(s) 24, 38, 524
CfoI GCGC 1 cut(s) 813
Cfr10I RCCGGY 1 cut(s) 909
Cfr13I GGNCC 3 cut(s) 593, 631, 678
Csp6I GTAC 3 cut(s) 344, 624, 698
CspAI ACCGGT 1 cut(s) 909
CviAII CATG 4 cut(s) 118, 245, 412, 766
CviQI GTAC 3 cut(s) 344, 624, 698
DdeI CTNAG 6 cut(s) 162, 285, 486, 525, 806, 877
DinI GGCGCC 1 cut(s) 812
DpnI GATC 2 cut(s) 561, 792
DpnII GATC 2 cut(s) 559, 790
DriI GACNNNNNGTC 1 cut(s) 573
EaeI YGGCCR 1 cut(s) 408
Eam1105I GACNNNNNGTC 1 cut(s) 573
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 876
Eco130I CCWWGG 2 cut(s) 117, 627
Eco24I GRGCYC 1 cut(s) 878
Eco47I GGWCC 1 cut(s) 631
Eco53kI GAGCTC 1 cut(s) 876
Eco57I CTGAAG 4 cut(s) 450, 534, 732, 768
Eco81I CCTNAGG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 876
EcoO109I RGGNCCY 1 cut(s) 678
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 2 cut(s) 117, 627
EcoT22I ATGCAT 1 cut(s) 767
EcoT38I GRGCYC 1 cut(s) 878
EgeI GGCGCC 1 cut(s) 812
EheI GGCGCC 1 cut(s) 812
ErhI CCWWGG 2 cut(s) 117, 627
FaeI CATG 4 cut(s) 121, 248, 415, 769
FalI AAGNNNNNCTT 6 cut(s) 173, 205, 342, 374, 705, 737
FaqI GGGAC 2 cut(s) 346, 754
FatI CATG 4 cut(s) 117, 244, 411, 765
FauNDI CATATG 2 cut(s) 730, 761
FblI GTMKAC 1 cut(s) 608
Fnu4HI GCNGC 1 cut(s) 402
FokI GGATG 3 cut(s) 134, 240, 458
FriOI GRGCYC 1 cut(s) 878
Fsp4HI GCNGC 1 cut(s) 402
FspBI CTAG 2 cut(s) 794, 896
GlaI GCGC 1 cut(s) 812
GluI GCNGC 1 cut(s) 402
GsuI CTGGAG 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 814
HaeIII GGCC 3 cut(s) 410, 594, 679
HapII CCGG 6 cut(s) 267, 552, 682, 740, 910, 952
HhaI GCGC 1 cut(s) 813
Hin1I GRCGYC 1 cut(s) 811
Hin1II CATG 4 cut(s) 121, 248, 415, 769
Hin6I GCGC 1 cut(s) 811
HinP1I GCGC 1 cut(s) 811
HincII GTYRAC 2 cut(s) 609, 694
HindII GTYRAC 2 cut(s) 609, 694
HindIII AAGCTT 3 cut(s) 68, 146, 641
HinfI GANTC 6 cut(s) 203, 415, 449, 521, 662, 935
HpaII CCGG 6 cut(s) 267, 552, 682, 740, 910, 952
HphI GGTGA 1 cut(s) 883
Hpy166II GTNNAC 3 cut(s) 97, 609, 694
Hpy188I TCNGA 8 cut(s) 12, 110, 163, 463, 526, 822, 837, 880
Hpy188III TCNNGA 2 cut(s) 284, 359
Hpy8I GTNNAC 3 cut(s) 97, 609, 694
HpyAV CCTTC 4 cut(s) 425, 643, 647, 743
HpyCH4III ACNGT 3 cut(s) 106, 571, 613
HpyCH4IV ACGT 2 cut(s) 422, 696
HpyCH4V TGCA 8 cut(s) 90, 477, 535, 542, 604, 620, 765, 889
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 407
HpyF3I CTNAG 6 cut(s) 162, 285, 486, 525, 806, 877
HpySE526I ACGT 2 cut(s) 422, 696
Hsp92I GRCGYC 1 cut(s) 811
Hsp92II CATG 4 cut(s) 121, 248, 415, 769
HspAI GCGC 1 cut(s) 811
KasI GGCGCC 1 cut(s) 810
Kzo9I GATC 2 cut(s) 559, 790
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 1 cut(s) 388
LweI GCATC 2 cut(s) 167, 362
MaeI CTAG 2 cut(s) 794, 896
MaeII ACGT 2 cut(s) 422, 696
MaeIII GTNAC 4 cut(s) 28, 565, 663, 905
MalI GATC 2 cut(s) 561, 792
MboI GATC 2 cut(s) 559, 790
MboII GAAGA 5 cut(s) 65, 697, 765, 850, 872
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 790
MhlI GDGCHC 1 cut(s) 878
MlsI TGGCCA 1 cut(s) 410
MluCI AATT 5 cut(s) 219, 312, 597, 823, 829
MluNI TGGCCA 1 cut(s) 410
Mly113I GGCGCC 1 cut(s) 811
MlyI GAGTC 4 cut(s) 197, 458, 671, 929
MmeI TCCRAC 1 cut(s) 881
MnlI CCTC 9 cut(s) 35, 274, 280, 403, 520, 644, 736, 801, 949
Mox20I TGGCCA 1 cut(s) 410
Mph1103I ATGCAT 1 cut(s) 767
MroXI GAANNNNTTC 2 cut(s) 176, 717
MscI TGGCCA 1 cut(s) 410
MseI TTAA 7 cut(s) 66, 311, 315, 327, 339, 348, 780
MslI CAYNNNNRTG 1 cut(s) 764
Msp20I TGGCCA 1 cut(s) 410
MspI CCGG 6 cut(s) 267, 552, 682, 740, 910, 952
MspR9I CCNGG 5 cut(s) 58, 268, 553, 682, 953
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 2 cut(s) 51, 407
NarI GGCGCC 1 cut(s) 811
NciI CCSGG 4 cut(s) 268, 553, 682, 953
NcoI CCATGG 1 cut(s) 117
NdeI CATATG 2 cut(s) 730, 761
NdeII GATC 2 cut(s) 559, 790
NlaIII CATG 4 cut(s) 121, 248, 415, 769
NlaIV GGNNCC 4 cut(s) 55, 103, 680, 812
NmuCI GTSAC 2 cut(s) 565, 663
NsiI ATGCAT 1 cut(s) 767
NspI RCATGY 1 cut(s) 769
PacI TTAATTAA 1 cut(s) 315
PdmI GAANNNNTTC 2 cut(s) 176, 717
PfeI GAWTC 2 cut(s) 415, 521
Pfl23II CGTACG 1 cut(s) 697
PfoI TCCNGGA 1 cut(s) 266
PinAI ACCGGT 1 cut(s) 909
PkrI GCNGC 1 cut(s) 403
PleI GAGTC 4 cut(s) 197, 457, 670, 929
PluTI GGCGCC 1 cut(s) 814
PpsI GAGTC 4 cut(s) 197, 457, 670, 929
Ppu21I YACGTR 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 878
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspLI CGTACG 1 cut(s) 697
PspN4I GGNNCC 4 cut(s) 55, 103, 680, 812
PspPI GGNCC 3 cut(s) 593, 631, 678
PstI CTGCAG 3 cut(s) 479, 537, 606
PstNI CAGNNNCTG 3 cut(s) 24, 38, 524
PsuI RGATCY 1 cut(s) 790
RsaI GTAC 3 cut(s) 345, 625, 699
RsaNI GTAC 3 cut(s) 344, 624, 698
RseI CAYNNNNRTG 1 cut(s) 764
SacI GAGCTC 1 cut(s) 878
SalI GTCGAC 1 cut(s) 607
SaqAI TTAA 7 cut(s) 66, 311, 315, 327, 339, 348, 780
SatI GCNGC 1 cut(s) 402
Sau3AI GATC 2 cut(s) 559, 790
Sau96I GGNCC 3 cut(s) 593, 631, 678
SchI GAGTC 4 cut(s) 197, 458, 671, 929
ScrFI CCNGG 5 cut(s) 58, 268, 553, 682, 953
SduI GDGCHC 1 cut(s) 878
SfaNI GCATC 2 cut(s) 167, 362
SfcI CTRYAG 3 cut(s) 475, 533, 602
SfoI GGCGCC 1 cut(s) 812
SinI GGWCC 1 cut(s) 631
SmiMI CAYNNNNRTG 1 cut(s) 764
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 5 cut(s) 219, 312, 597, 823, 829
SsiI CCGC 1 cut(s) 185
SspDI GGCGCC 1 cut(s) 810
SspMI CTAG 2 cut(s) 794, 896
SstI GAGCTC 1 cut(s) 878
StyD4I CCNGG 5 cut(s) 56, 266, 551, 680, 951
StyI CCWWGG 2 cut(s) 117, 627
TaaI ACNGT 3 cut(s) 106, 571, 613
TaiI ACGT 2 cut(s) 425, 699
TaqI TCGA 1 cut(s) 608
TasI AATT 5 cut(s) 219, 312, 597, 823, 829
TatI WGTACW 1 cut(s) 343
TfiI GAWTC 2 cut(s) 415, 521
Tru1I TTAA 7 cut(s) 66, 311, 315, 327, 339, 348, 780
Tru9I TTAA 7 cut(s) 66, 311, 315, 327, 339, 348, 780
TscAI CASTG 2 cut(s) 479, 672
TseFI GTSAC 2 cut(s) 565, 663
TseI GCWGC 1 cut(s) 401
Tsp45I GTSAC 2 cut(s) 565, 663
TspDTI ATGAA 2 cut(s) 233, 428
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 2 cut(s) 479, 672
VpaK11BI GGWCC 1 cut(s) 631
XceI RCATGY 1 cut(s) 769
XmiI GTMKAC 1 cut(s) 608
XmnI GAANNNNTTC 2 cut(s) 176, 717
XspI CTAG 2 cut(s) 794, 896
Zsp2I ATGCAT 1 cut(s) 767
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.