MD06G1003500.v1.1

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002886
Physical Location & Seq
Forward (+)
408324 .. 411699
3376 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1003500.v1.1.491

Sequence Viewer

Length: 1020 bp
ATGGCGGTTGAGAAGGGAAGATACTGTGTAACAGGAGCTGGAGGGTTTCTTGCGTCGTGGGTTGTTAAGCTTCTTCTTTCCAAGGATTCTATTATCCACGGAACCCTCAGAGACCCCTGTATGTATCCTCTATCCAACCATTTTCCTTTCTTTCTTTCGACTGTTTGCACTTGCAGTGACTACTGTTCCATCGATTCTAATGACAAGCACGCCCACTTGAAAAAACTTGACAAGGCATCTGAGAACCTGAAACTATTCAAAGCGGACTTGTTGGACTATGAATCCCTTCACACAGCGATTCAAGGATGTGATGGAGTGTTTCATGTTGCCAGTCCAGTTCCCTACGACCCTGTAGAATTGATTGAACCTGCCGTGAAGGGAACACTTAATGTACTTAAAGCATGCCTTGAAGCAAAAGTCAAGCGAGTTGTTTTCGTGTCATCTGCAGCTTCTCTTGTCATGAATCCTAAGTGGTCTCAAGGCCAAGTGTTGAATGAAACTTGTTGGTCAGACAAGGAATACTGCAGAAGTACTAAGAATTGGTATTGTCTTTCAAAAACTGAAGCAGAATATGAAGCCCTGGAGTATGCAAGAATAAATGGGCTTGACTTGGTAACTGTTTGTCCTACACTTATTATGGGGCCAATTCTGCAGTCCACTGTAAATGCAAGTACCTTGGTCCTCATCAAGCTTTTGAAGGAAGGGTATGAGTCACTGGAAAACAAGCTGCGGATGATAGTTGATGTGCGGGATGTAGCTGAAGCAGTCATTATGGTGTATGAGAAGCCTGAGGCTGAAGGAAGATACATATGCACCGCTCACAATATCAAGACGACAGATCTGGTTGAAGAGCTGAAGAGCATTTATCCTAATTACAGTTATCCTAAGAACTTTGTTGAAGTAGAAGAACAACCAAGGTTGAGCTCGGAGAAACTTCAGAGGCTGGGTTTGAGTTTCCGACCATTGAAAGAGACGCTTACTGGCTCAGTTGAAAGCTACAAAGAGGCTGGACTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

340

Amino Acids

37.96

Weight (kDa)

5.89

Isoelectric Point (pI)

34.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 173 1.6e-08 NmrA-like family
Epimerase PF01370 9 - 266 2.6e-21 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 257 1.6e-15 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 62 - 284 2.1e-08 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 73 - 210 3.5e-10 NAD(P)H-binding
Polysacc_synt_2 PF02719 81 - 148 2.1e-06 Polysaccharide biosynthesis protein
NAD_binding_4 PF07993 91 - 222 3.2e-09 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 376
AccBSI CCGCTC 1 cut(s) 818
AciI CCGC 5 cut(s) 5, 263, 730, 748, 816
AcuI CTGAAG 5 cut(s) 582, 780, 816, 875, 920
AfaI GTAC 3 cut(s) 393, 532, 673
AjnI CCWGG 1 cut(s) 579
AluBI AGCT 9 cut(s) 38, 70, 449, 691, 727, 758, 853, 924, 996
AluI AGCT 9 cut(s) 38, 70, 449, 691, 727, 758, 853, 924, 996
Alw21I GWGCWC 1 cut(s) 926
Alw26I GTCTC 3 cut(s) 105, 480, 965
AlwNI CAGNNNCTG 2 cut(s) 38, 943
AoxI GGCC 2 cut(s) 481, 641
ApeKI GCWGC 2 cut(s) 446, 727
Asp700I GAANNNNTTC 2 cut(s) 254, 285
AspS9I GGNCC 2 cut(s) 641, 679
AvaII GGWCC 1 cut(s) 679
AxyI CCTNAGG 1 cut(s) 789
BanII GRGCYC 1 cut(s) 926
BarI GAAGNNNNNNTAC 1 cut(s) 37
Bbv12I GWGCWC 1 cut(s) 926
BbvI GCAGC 2 cut(s) 458, 714
BccI CCATC 2 cut(s) 197, 305
BceAI ACGGC 1 cut(s) 356
BciT130I CCWGG 1 cut(s) 581
BciVI GTATCC 1 cut(s) 135
BcoDI GTCTC 3 cut(s) 105, 480, 965
BfmI CTRYAG 4 cut(s) 351, 444, 523, 650
BfuAI ACCTGC 1 cut(s) 376
BfuI GTATCC 1 cut(s) 135
BglII AGATCT 1 cut(s) 838
BisI GCNGC 2 cut(s) 447, 728
BlsI GCNGC 2 cut(s) 448, 729
BmcAI AGTACT 1 cut(s) 532
Bme1390I CCNGG 1 cut(s) 581
Bme18I GGWCC 1 cut(s) 679
BmgT120I GGNCC 2 cut(s) 641, 679
BmiI GGNNCC 2 cut(s) 103, 642
BmrFI CCNGG 1 cut(s) 581
BmsI GCATC 1 cut(s) 245
BpmI CTGGAG 2 cut(s) 60, 602
BpuEI CTTGAG 1 cut(s) 462
Bsa29I ATCGAT 1 cut(s) 192
BsaI GGTCTC 2 cut(s) 105, 480
BsaJI CCNNGG 5 cut(s) 81, 97, 579, 675, 914
Bse1I ACTGG 4 cut(s) 330, 335, 720, 985
Bse21I CCTNAGG 1 cut(s) 789
BseBI CCWGG 1 cut(s) 581
BseCI ATCGAT 1 cut(s) 192
BseDI CCNNGG 5 cut(s) 81, 97, 579, 675, 914
BseGI GGATG 3 cut(s) 311, 738, 757
BseMII CTCAG 4 cut(s) 121, 231, 780, 999
BseNI ACTGG 4 cut(s) 330, 335, 720, 985
BseXI GCAGC 2 cut(s) 458, 714
BseYI CCCAGC 1 cut(s) 943
BshFI GGCC 2 cut(s) 483, 643
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 1 cut(s) 926
BsmAI GTCTC 3 cut(s) 105, 480, 965
BsmBI CGTCTC 1 cut(s) 965
BsnI GGCC 2 cut(s) 483, 643
Bso31I GGTCTC 2 cut(s) 105, 480
Bsp1286I GDGCHC 1 cut(s) 926
Bsp143I GATC 1 cut(s) 838
BspACI CCGC 5 cut(s) 5, 263, 730, 748, 816
BspANI GGCC 2 cut(s) 483, 643
BspCNI CTCAG 4 cut(s) 120, 232, 781, 998
BspDI ATCGAT 1 cut(s) 192
BspHI TCATGA 1 cut(s) 459
BspLI GGNNCC 2 cut(s) 103, 642
BspMAI CTGCAG 3 cut(s) 448, 527, 654
BspMI ACCTGC 1 cut(s) 376
BspQI GCTCTTC 2 cut(s) 843, 851
BspTNI GGTCTC 2 cut(s) 105, 480
BsrBI CCGCTC 1 cut(s) 818
BsrI ACTGG 4 cut(s) 330, 335, 720, 985
BssECI CCNNGG 5 cut(s) 81, 97, 579, 675, 914
BssMI GATC 1 cut(s) 838
BssT1I CCWWGG 3 cut(s) 81, 675, 914
Bst2UI CCWGG 1 cut(s) 581
Bst4CI ACNGT 6 cut(s) 26, 163, 185, 619, 661, 878
Bst6I CTCTTC 2 cut(s) 843, 851
BstC8I GCNNGC 2 cut(s) 210, 403
BstDEI CTNAG 7 cut(s) 107, 240, 468, 534, 789, 885, 985
BstDSI CCRYGG 1 cut(s) 97
BstF5I GGATG 3 cut(s) 311, 738, 757
BstKTI GATC 1 cut(s) 841
BstMAI GTCTC 3 cut(s) 105, 480, 965
BstMBI GATC 1 cut(s) 838
BstMWI GCNNNNNNNGC 1 cut(s) 649
BstNI CCWGG 1 cut(s) 581
BstNSI RCATGY 1 cut(s) 405
BstSCI CCNGG 1 cut(s) 579
BstSFI CTRYAG 4 cut(s) 351, 444, 523, 650
BstV1I GCAGC 2 cut(s) 458, 714
BstX2I RGATCY 1 cut(s) 838
BstYI RGATCY 1 cut(s) 838
Bsu15I ATCGAT 1 cut(s) 192
Bsu36I CCTNAGG 1 cut(s) 789
BsuI GTATCC 1 cut(s) 135
BsuRI GGCC 2 cut(s) 483, 643
BsuTUI ATCGAT 1 cut(s) 192
BtgI CCRYGG 1 cut(s) 97
BtsCI GGATG 3 cut(s) 311, 738, 757
BtsI GCAGTG 1 cut(s) 181
BtsIMutI CAGTG 3 cut(s) 181, 657, 713
BveI ACCTGC 1 cut(s) 376
Cac8I GCNNGC 2 cut(s) 210, 403
CaiI CAGNNNCTG 2 cut(s) 38, 943
CciI TCATGA 1 cut(s) 459
Cfr13I GGNCC 2 cut(s) 641, 679
ClaI ATCGAT 1 cut(s) 192
CseI GACGC 2 cut(s) 42, 982
Csp6I GTAC 3 cut(s) 392, 531, 672
CviAII CATG 3 cut(s) 323, 402, 460
CviQI GTAC 3 cut(s) 392, 531, 672
DdeI CTNAG 7 cut(s) 107, 240, 468, 534, 789, 885, 985
DpnI GATC 1 cut(s) 840
DpnII GATC 1 cut(s) 838
Eam1104I CTCTTC 2 cut(s) 843, 851
EarI CTCTTC 2 cut(s) 843, 851
Ecl136II GAGCTC 1 cut(s) 924
Eco130I CCWWGG 3 cut(s) 81, 675, 914
Eco24I GRGCYC 1 cut(s) 926
Eco31I GGTCTC 2 cut(s) 105, 480
Eco47I GGWCC 1 cut(s) 679
Eco53kI GAGCTC 1 cut(s) 924
Eco57I CTGAAG 5 cut(s) 582, 780, 816, 875, 920
Eco81I CCTNAGG 1 cut(s) 789
EcoICRI GAGCTC 1 cut(s) 924
EcoRII CCWGG 1 cut(s) 579
EcoT14I CCWWGG 3 cut(s) 81, 675, 914
EcoT38I GRGCYC 1 cut(s) 926
ErhI CCWWGG 3 cut(s) 81, 675, 914
Esp3I CGTCTC 1 cut(s) 965
FaeI CATG 3 cut(s) 326, 405, 463
FalI AAGNNNNNCTT 4 cut(s) 390, 422, 960, 992
FatI CATG 3 cut(s) 322, 401, 459
FauI CCCGC 1 cut(s) 741
FauNDI CATATG 1 cut(s) 809
Fnu4HI GCNGC 2 cut(s) 447, 728
FokI GGATG 3 cut(s) 318, 745, 764
FriOI GRGCYC 1 cut(s) 926
Fsp4HI GCNGC 2 cut(s) 447, 728
GluI GCNGC 2 cut(s) 447, 728
GsaI CCCAGC 1 cut(s) 947
GsuI CTGGAG 2 cut(s) 60, 602
HaeIII GGCC 2 cut(s) 483, 643
HgaI GACGC 2 cut(s) 42, 982
Hin1II CATG 3 cut(s) 326, 405, 463
HindIII AAGCTT 2 cut(s) 68, 689
HinfI GANTC 6 cut(s) 86, 194, 281, 298, 463, 710
Hpy166II GTNNAC 1 cut(s) 657
Hpy188I TCNGA 7 cut(s) 110, 241, 511, 928, 939, 959, 1019
Hpy188III TCNNGA 2 cut(s) 460, 829
Hpy8I GTNNAC 1 cut(s) 657
Hpy99I CGWCG 1 cut(s) 58
HpyAV CCTTC 6 cut(s) 7, 296, 370, 691, 695, 791
HpyCH4III ACNGT 6 cut(s) 26, 163, 185, 619, 661, 878
HpyCH4V TGCA 8 cut(s) 168, 174, 446, 525, 590, 652, 668, 813
HpyF10VI GCNNNNNNNGC 1 cut(s) 649
HpyF3I CTNAG 7 cut(s) 107, 240, 468, 534, 789, 885, 985
Hsp92II CATG 3 cut(s) 326, 405, 463
Kzo9I GATC 1 cut(s) 838
LguI GCTCTTC 2 cut(s) 843, 851
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 2 cut(s) 458, 714
LweI GCATC 1 cut(s) 245
MaeIII GTNAC 4 cut(s) 28, 176, 613, 711
MalI GATC 1 cut(s) 840
MbiI CCGCTC 1 cut(s) 818
MboI GATC 1 cut(s) 838
MboII GAAGA 6 cut(s) 30, 65, 813, 860, 868, 917
MflI RGATCY 1 cut(s) 838
MhlI GDGCHC 1 cut(s) 926
MluCI AATT 4 cut(s) 356, 538, 645, 871
MlyI GAGTC 1 cut(s) 719
MmeI TCCRAC 3 cut(s) 159, 252, 982
MnlI CCTC 7 cut(s) 35, 116, 138, 692, 784, 933, 997
MroXI GAANNNNTTC 2 cut(s) 254, 285
MseI TTAA 3 cut(s) 66, 387, 396
MslI CAYNNNNRTG 1 cut(s) 773
MspR9I CCNGG 1 cut(s) 581
MvaI CCWGG 1 cut(s) 581
MwoI GCNNNNNNNGC 1 cut(s) 649
NdeI CATATG 1 cut(s) 809
NdeII GATC 1 cut(s) 838
NlaIII CATG 3 cut(s) 326, 405, 463
NlaIV GGNNCC 2 cut(s) 103, 642
NmuCI GTSAC 2 cut(s) 176, 711
NspI RCATGY 1 cut(s) 405
PaeI GCATGC 1 cut(s) 405
PagI TCATGA 1 cut(s) 459
PciSI GCTCTTC 2 cut(s) 843, 851
PdmI GAANNNNTTC 2 cut(s) 254, 285
PfeI GAWTC 5 cut(s) 86, 194, 281, 298, 463
PkrI GCNGC 2 cut(s) 448, 729
PleI GAGTC 1 cut(s) 718
PpsI GAGTC 1 cut(s) 718
Psp124BI GAGCTC 1 cut(s) 926
Psp6I CCWGG 1 cut(s) 579
PspFI CCCAGC 1 cut(s) 943
PspGI CCWGG 1 cut(s) 579
PspN4I GGNNCC 2 cut(s) 103, 642
PspPI GGNCC 2 cut(s) 641, 679
PstI CTGCAG 3 cut(s) 448, 527, 654
PstNI CAGNNNCTG 2 cut(s) 38, 943
PsuI RGATCY 1 cut(s) 838
RsaI GTAC 3 cut(s) 393, 532, 673
RsaNI GTAC 3 cut(s) 392, 531, 672
RseI CAYNNNNRTG 1 cut(s) 773
SacI GAGCTC 1 cut(s) 926
SapI GCTCTTC 2 cut(s) 843, 851
SaqAI TTAA 3 cut(s) 66, 387, 396
SatI GCNGC 2 cut(s) 447, 728
Sau3AI GATC 1 cut(s) 838
Sau96I GGNCC 2 cut(s) 641, 679
ScaI AGTACT 1 cut(s) 532
SchI GAGTC 1 cut(s) 719
ScrFI CCNGG 1 cut(s) 581
SduI GDGCHC 1 cut(s) 926
SfaNI GCATC 1 cut(s) 245
SfcI CTRYAG 4 cut(s) 351, 444, 523, 650
SinI GGWCC 1 cut(s) 679
SmiMI CAYNNNNRTG 1 cut(s) 773
SmlI CTYRAG 1 cut(s) 477
SmoI CTYRAG 1 cut(s) 477
SphI GCATGC 1 cut(s) 405
Sse9I AATT 4 cut(s) 356, 538, 645, 871
SsiI CCGC 5 cut(s) 5, 263, 730, 748, 816
SstI GAGCTC 1 cut(s) 926
StyD4I CCNGG 1 cut(s) 579
StyI CCWWGG 3 cut(s) 81, 675, 914
TaaI ACNGT 6 cut(s) 26, 163, 185, 619, 661, 878
TaqI TCGA 2 cut(s) 158, 192
TasI AATT 4 cut(s) 356, 538, 645, 871
TatI WGTACW 2 cut(s) 391, 530
TfiI GAWTC 5 cut(s) 86, 194, 281, 298, 463
Tru1I TTAA 3 cut(s) 66, 387, 396
Tru9I TTAA 3 cut(s) 66, 387, 396
TscAI CASTG 3 cut(s) 181, 664, 720
TseFI GTSAC 2 cut(s) 176, 711
TseI GCWGC 2 cut(s) 446, 727
Tsp45I GTSAC 2 cut(s) 176, 711
TspDTI ATGAA 5 cut(s) 294, 311, 476, 510, 588
TspGWI ACGGA 1 cut(s) 114
TspRI CASTG 3 cut(s) 181, 664, 720
VpaK11BI GGWCC 1 cut(s) 679
XceI RCATGY 1 cut(s) 405
XmnI GAANNNNTTC 2 cut(s) 254, 285
ZrmI AGTACT 1 cut(s) 532
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.