Rw7G025720

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0002886
Physical Location & Seq
Reverse (-)
32305672 .. 32308439
2768 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G025720.1

Sequence Viewer

Length: 960 bp
ATGGCAGTTCAGAATGGAAGGTTCTGTGTTACAGGGGCTGGAGGCTTCGTTGGCTCCTGGGTCGTTAAGCTTCTTCTTTCCAAGAACTGCATAGTCCACGGAACCGTCAGAGAACCCACGGATGACAAGAACTCTCACTTGAGAAAGCTTGACAAGGCATCTGAGAACCTGAAACTATTCAAGGCAGACTTATTGGATTATGACTCCCTTCGCTCGGCAATTGAAGGATGTGATGGAGTATTTCATGTTGCCAGTCCCGCTCCCTCCGGCAGTGTGCCAAATCCTGAGATTGAGTTAATTGAACCTGCTGTTAAGGGGACACTTAATGTACTTAAAGCATCACTTGAAACAAAAGTCAAGCGAGTTGTTTCTGTGTCCTCTATTGCTGCTGTGGCCGCGAATCCTACGTTGGCTGAAGGTCAAGTTTTGGATGAGTCTTGTTGGTCAGATAAGGAATACTGCAGAAATACTGAGAACTGGTATTGCCTTTCAAAAACTGAAGCAGAATCTGAGGCTCTGCAGTTTGCAAAAACAACCGGGATTGATCTTGTGACTGTTTGTCCTACACTTATACTTGGCCCAATTCTGCAGTCGACTGTAAATGCAAGTACCTTCATCTTCATCAAGCTTTTGAAGGAAGGGAAGGAGTCACTGGAAAACAGGCCTCAGAAGATAGTTGACGTACGGGATTTAGCTGAAGCACTTCTTATGGCATATGAGAAGCCGGAGGCTGAAGGAAGATACATATGCATGTCCCACAATGTTAAGAATGGGGATCTAGTTGAGAAGCTGAGGCGCCTTTATCCTGATTACAATTATCCGAAGAACTTTATTGAAGTAGGCAAAGACAGACAGGTGAGCTCAGAGAAATTGCAAAGGCTAGGTTGGAGTTACAGGTCATTGGACGATACTCTCATTGACTCTGTTGAAAGCTACCGGGAGGCTGGACTATTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

319

Amino Acids

35.31

Weight (kDa)

5.48

Isoelectric Point (pI)

39.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 123 8.4e-08 NmrA-like family
KR PF08659 7 - 136 4.8e-07 KR domain
Epimerase PF01370 9 - 245 9.5e-28 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 134 9.2e-10 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 10 - 202 2.7e-19 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 263 8.4e-16 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 10 - 201 1.1e-08 Male sterility protein
NAD_binding_10 PF13460 12 - 133 4.5e-14 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 313
AccB1I GGYRCC 1 cut(s) 795
AccBSI CCGCTC 1 cut(s) 260
AccI GTMKAC 1 cut(s) 593
AccII CGCG 1 cut(s) 398
AciI CCGC 2 cut(s) 258, 396
AclWI GGATC 1 cut(s) 783
AcoI YGGCCR 1 cut(s) 393
AcuI CTGAAG 4 cut(s) 435, 519, 717, 753
AcyI GRCGYC 1 cut(s) 796
AfaI GTAC 3 cut(s) 330, 610, 684
AfiI CCNNNNNNNGG 1 cut(s) 214
AgsI TTSAA 8 cut(s) 181, 224, 302, 347, 492, 634, 836, 929
AhdI GACNNNNNGTC 1 cut(s) 558
AjnI CCWGG 1 cut(s) 56
AjuI GAANNNNNNNTTGG 2 cut(s) 392, 424
AluBI AGCT 7 cut(s) 70, 148, 628, 695, 790, 861, 933
AluI AGCT 7 cut(s) 70, 148, 628, 695, 790, 861, 933
Alw21I GWGCWC 1 cut(s) 863
AlwI GGATC 1 cut(s) 783
AlwNI CAGNNNCTG 2 cut(s) 38, 509
AoxI GGCC 3 cut(s) 393, 577, 662
ApeKI GCWGC 1 cut(s) 386
Asp700I GAANNNNTTC 2 cut(s) 176, 702
AspLEI GCGC 1 cut(s) 798
AspS9I GGNCC 1 cut(s) 578
AsuC2I CCSGG 2 cut(s) 538, 938
AsuHPI GGTGA 1 cut(s) 868
BanI GGYRCC 1 cut(s) 795
BanII GRGCYC 1 cut(s) 863
Bbv12I GWGCWC 1 cut(s) 863
BbvCI CCTCAGC 1 cut(s) 791
BbvI GCAGC 1 cut(s) 373
BccI CCATC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 2 cut(s) 538, 938
BfaI CTAG 2 cut(s) 779, 881
BfmI CTRYAG 3 cut(s) 460, 518, 587
BfoI RGCGCY 1 cut(s) 799
BfuAI ACCTGC 1 cut(s) 313
BisI GCNGC 2 cut(s) 387, 396
BlsI GCNGC 2 cut(s) 388, 397
Bme1390I CCNGG 3 cut(s) 58, 538, 938
BmeRI GACNNNNNGTC 1 cut(s) 558
BmgT120I GGNCC 1 cut(s) 578
BmiI GGNNCC 3 cut(s) 55, 103, 797
BmrFI CCNGG 3 cut(s) 58, 538, 938
BmsI GCATC 2 cut(s) 167, 347
BpmI CTGGAG 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 791
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 2 cut(s) 538, 938
BsaHI GRCGYC 1 cut(s) 796
BsaJI CCNNGG 3 cut(s) 57, 97, 117
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 1 cut(s) 214
Bse1I ACTGG 3 cut(s) 252, 482, 657
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 3 cut(s) 57, 97, 117
BseGI GGATG 3 cut(s) 127, 233, 436
BseLI CCNNNNNNNGG 1 cut(s) 214
BseMII CTCAG 7 cut(s) 153, 276, 462, 501, 680, 782, 876
BseNI ACTGG 3 cut(s) 252, 482, 657
BseXI GCAGC 1 cut(s) 373
Bsh1236I CGCG 1 cut(s) 398
BshFI GGCC 3 cut(s) 395, 579, 664
BshNI GGYRCC 1 cut(s) 795
BsiHKAI GWGCWC 1 cut(s) 863
BsiSI CCGG 4 cut(s) 267, 537, 725, 937
BsiWI CGTACG 1 cut(s) 682
BslFI GGGAC 3 cut(s) 240, 331, 739
BslI CCNNNNNNNGG 1 cut(s) 214
BsmFI GGGAC 3 cut(s) 240, 331, 739
BsnI GGCC 3 cut(s) 395, 579, 664
Bsp1286I GDGCHC 1 cut(s) 863
Bsp143I GATC 2 cut(s) 544, 775
BspACI CCGC 2 cut(s) 258, 396
BspANI GGCC 3 cut(s) 395, 579, 664
BspCNI CTCAG 7 cut(s) 154, 277, 463, 502, 679, 783, 875
BspFNI CGCG 1 cut(s) 398
BspLI GGNNCC 3 cut(s) 55, 103, 797
BspMAI CTGCAG 3 cut(s) 464, 522, 591
BspMI ACCTGC 1 cut(s) 313
BspPI GGATC 1 cut(s) 783
BspT107I GGYRCC 1 cut(s) 795
BsrBI CCGCTC 1 cut(s) 260
BsrI ACTGG 3 cut(s) 252, 482, 657
BssECI CCNNGG 3 cut(s) 57, 97, 117
BssMI GATC 2 cut(s) 544, 775
BssNI GRCGYC 1 cut(s) 796
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 3 cut(s) 106, 556, 598
BstACI GRCGYC 1 cut(s) 796
BstDEI CTNAG 7 cut(s) 162, 285, 471, 510, 666, 791, 862
BstDSI CCRYGG 2 cut(s) 97, 117
BstF5I GGATG 3 cut(s) 127, 233, 436
BstFNI CGCG 1 cut(s) 398
BstH2I RGCGCY 1 cut(s) 799
BstHHI GCGC 1 cut(s) 798
BstKTI GATC 2 cut(s) 547, 778
BstMBI GATC 2 cut(s) 544, 775
BstMWI GCNNNNNNNGC 4 cut(s) 51, 257, 392, 395
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 754
BstSCI CCNGG 3 cut(s) 56, 536, 936
BstSFI CTRYAG 3 cut(s) 460, 518, 587
BstUI CGCG 1 cut(s) 398
BstV1I GCAGC 1 cut(s) 373
BstX2I RGATCY 1 cut(s) 775
BstYI RGATCY 1 cut(s) 775
BsuRI GGCC 3 cut(s) 395, 579, 664
BtgI CCRYGG 2 cut(s) 97, 117
BtsCI GGATG 3 cut(s) 127, 233, 436
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 2 cut(s) 277, 650
BveI ACCTGC 1 cut(s) 313
CaiI CAGNNNCTG 2 cut(s) 38, 509
CfoI GCGC 1 cut(s) 798
Cfr13I GGNCC 1 cut(s) 578
Csp6I GTAC 3 cut(s) 329, 609, 683
CviAII CATG 2 cut(s) 245, 751
CviQI GTAC 3 cut(s) 329, 609, 683
DdeI CTNAG 7 cut(s) 162, 285, 471, 510, 666, 791, 862
DinI GGCGCC 1 cut(s) 797
DpnI GATC 2 cut(s) 546, 777
DpnII GATC 2 cut(s) 544, 775
DriI GACNNNNNGTC 1 cut(s) 558
EaeI YGGCCR 1 cut(s) 393
Eam1105I GACNNNNNGTC 1 cut(s) 558
Ecl136II GAGCTC 1 cut(s) 861
Eco147I AGGCCT 1 cut(s) 664
Eco24I GRGCYC 1 cut(s) 863
Eco53kI GAGCTC 1 cut(s) 861
Eco57I CTGAAG 4 cut(s) 435, 519, 717, 753
EcoICRI GAGCTC 1 cut(s) 861
EcoRII CCWGG 1 cut(s) 56
EcoT22I ATGCAT 1 cut(s) 752
EcoT38I GRGCYC 1 cut(s) 863
EgeI GGCGCC 1 cut(s) 797
EheI GGCGCC 1 cut(s) 797
FaeI CATG 2 cut(s) 248, 754
FalI AAGNNNNNCTT 6 cut(s) 173, 205, 327, 359, 690, 722
FaqI GGGAC 3 cut(s) 240, 331, 739
FatI CATG 2 cut(s) 244, 750
FauI CCCGC 1 cut(s) 265
FauNDI CATATG 2 cut(s) 715, 746
FblI GTMKAC 1 cut(s) 593
Fnu4HI GCNGC 2 cut(s) 387, 396
FokI GGATG 3 cut(s) 134, 240, 443
FriOI GRGCYC 1 cut(s) 863
Fsp4HI GCNGC 2 cut(s) 387, 396
FspBI CTAG 2 cut(s) 779, 881
GlaI GCGC 1 cut(s) 797
GluI GCNGC 2 cut(s) 387, 396
GsuI CTGGAG 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 799
HaeIII GGCC 3 cut(s) 395, 579, 664
HapII CCGG 4 cut(s) 267, 537, 725, 937
HhaI GCGC 1 cut(s) 798
Hin1I GRCGYC 1 cut(s) 796
Hin1II CATG 2 cut(s) 248, 754
Hin6I GCGC 1 cut(s) 796
HinP1I GCGC 1 cut(s) 796
HincII GTYRAC 2 cut(s) 594, 679
HindII GTYRAC 2 cut(s) 594, 679
HindIII AAGCTT 3 cut(s) 68, 146, 626
HinfI GANTC 6 cut(s) 203, 400, 434, 506, 647, 920
HpaII CCGG 4 cut(s) 267, 537, 725, 937
HphI GGTGA 1 cut(s) 868
Hpy166II GTNNAC 3 cut(s) 97, 594, 679
Hpy188I TCNGA 8 cut(s) 12, 110, 163, 448, 511, 669, 822, 865
Hpy188III TCNNGA 2 cut(s) 284, 806
Hpy8I GTNNAC 3 cut(s) 97, 594, 679
HpyAV CCTTC 9 cut(s) 12, 218, 218, 410, 622, 628, 632, 637, 728
HpyCH4III ACNGT 3 cut(s) 106, 556, 598
HpyCH4IV ACGT 2 cut(s) 407, 681
HpyCH4V TGCA 8 cut(s) 90, 462, 520, 527, 589, 605, 750, 874
HpyF10VI GCNNNNNNNGC 4 cut(s) 51, 257, 392, 395
HpyF3I CTNAG 7 cut(s) 162, 285, 471, 510, 666, 791, 862
HpySE526I ACGT 2 cut(s) 407, 681
Hsp92I GRCGYC 1 cut(s) 796
Hsp92II CATG 2 cut(s) 248, 754
HspAI GCGC 1 cut(s) 796
KasI GGCGCC 1 cut(s) 795
Kzo9I GATC 2 cut(s) 544, 775
LmnI GCTCC 2 cut(s) 59, 265
Lsp1109I GCAGC 1 cut(s) 373
LweI GCATC 2 cut(s) 167, 347
MaeI CTAG 2 cut(s) 779, 881
MaeII ACGT 2 cut(s) 407, 681
MaeIII GTNAC 4 cut(s) 28, 550, 648, 890
MalI GATC 2 cut(s) 546, 777
MbiI CCGCTC 1 cut(s) 260
MboI GATC 2 cut(s) 544, 775
MboII GAAGA 5 cut(s) 65, 610, 682, 750, 835
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 775
MhlI GDGCHC 1 cut(s) 863
MluCI AATT 5 cut(s) 219, 297, 582, 814, 869
Mly113I GGCGCC 1 cut(s) 796
MlyI GAGTC 4 cut(s) 197, 443, 656, 914
MmeI TCCRAC 1 cut(s) 866
MnlI CCTC 8 cut(s) 35, 274, 388, 505, 675, 721, 786, 934
Mph1103I ATGCAT 1 cut(s) 752
MroXI GAANNNNTTC 2 cut(s) 176, 702
MseI TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
MslI CAYNNNNRTG 1 cut(s) 749
MspI CCGG 4 cut(s) 267, 537, 725, 937
MspR9I CCNGG 3 cut(s) 58, 538, 938
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 58
MvnI CGCG 1 cut(s) 398
MwoI GCNNNNNNNGC 4 cut(s) 51, 257, 392, 395
NarI GGCGCC 1 cut(s) 796
NciI CCSGG 2 cut(s) 538, 938
NdeI CATATG 2 cut(s) 715, 746
NdeII GATC 2 cut(s) 544, 775
NlaIII CATG 2 cut(s) 248, 754
NlaIV GGNNCC 3 cut(s) 55, 103, 797
NmeAIII GCCGAG 1 cut(s) 194
NmuCI GTSAC 2 cut(s) 550, 648
NsiI ATGCAT 1 cut(s) 752
NspI RCATGY 1 cut(s) 754
PceI AGGCCT 1 cut(s) 664
PdmI GAANNNNTTC 2 cut(s) 176, 702
PfeI GAWTC 2 cut(s) 400, 506
Pfl23II CGTACG 1 cut(s) 682
PkrI GCNGC 2 cut(s) 388, 397
PleI GAGTC 4 cut(s) 197, 442, 655, 914
PluTI GGCGCC 1 cut(s) 799
PpsI GAGTC 4 cut(s) 197, 442, 655, 914
Psp124BI GAGCTC 1 cut(s) 863
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspLI CGTACG 1 cut(s) 682
PspN4I GGNNCC 3 cut(s) 55, 103, 797
PspPI GGNCC 1 cut(s) 578
PstI CTGCAG 3 cut(s) 464, 522, 591
PstNI CAGNNNCTG 2 cut(s) 38, 509
PsuI RGATCY 1 cut(s) 775
RsaI GTAC 3 cut(s) 330, 610, 684
RsaNI GTAC 3 cut(s) 329, 609, 683
RseI CAYNNNNRTG 1 cut(s) 749
SacI GAGCTC 1 cut(s) 863
SalI GTCGAC 1 cut(s) 592
SaqAI TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
SatI GCNGC 2 cut(s) 387, 396
Sau3AI GATC 2 cut(s) 544, 775
Sau96I GGNCC 1 cut(s) 578
SchI GAGTC 4 cut(s) 197, 443, 656, 914
ScrFI CCNGG 3 cut(s) 58, 538, 938
SduI GDGCHC 1 cut(s) 863
SfaNI GCATC 2 cut(s) 167, 347
SfcI CTRYAG 3 cut(s) 460, 518, 587
SfoI GGCGCC 1 cut(s) 797
SmiMI CAYNNNNRTG 1 cut(s) 749
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 5 cut(s) 219, 297, 582, 814, 869
SseBI AGGCCT 1 cut(s) 664
SsiI CCGC 2 cut(s) 258, 396
SspDI GGCGCC 1 cut(s) 795
SspMI CTAG 2 cut(s) 779, 881
SstI GAGCTC 1 cut(s) 863
StuI AGGCCT 1 cut(s) 664
StyD4I CCNGG 3 cut(s) 56, 536, 936
TaaI ACNGT 3 cut(s) 106, 556, 598
TaiI ACGT 2 cut(s) 410, 684
TaqI TCGA 1 cut(s) 593
TasI AATT 5 cut(s) 219, 297, 582, 814, 869
TatI WGTACW 1 cut(s) 328
TauI GCSGC 1 cut(s) 398
TfiI GAWTC 2 cut(s) 400, 506
Tru1I TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
Tru9I TTAA 6 cut(s) 66, 296, 312, 324, 333, 765
TscAI CASTG 2 cut(s) 277, 657
TseFI GTSAC 2 cut(s) 550, 648
TseI GCWGC 1 cut(s) 386
Tsp45I GTSAC 2 cut(s) 550, 648
TspDTI ATGAA 3 cut(s) 233, 604, 610
TspGWI ACGGA 2 cut(s) 114, 134
TspRI CASTG 2 cut(s) 277, 657
XceI RCATGY 1 cut(s) 754
XmiI GTMKAC 1 cut(s) 593
XmnI GAANNNNTTC 2 cut(s) 176, 702
XspI CTAG 2 cut(s) 779, 881
Zsp2I ATGCAT 1 cut(s) 752
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.