MD06G1003200.v1.1

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0002886
Physical Location & Seq
Forward (+)
378971 .. 381379
2409 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1003200.v1.1.491

Sequence Viewer

Length: 864 bp
ATGATTAATAGTACTGTATTTGTGTTGTGCACAAACGCAGCTAATGACAAGCACGCCCACTTGAAAAAACTTGACAAGGCATCTGAGAACCTTAAACTATTCAAAGCGGACTTGTTGGACTATGAATCCCTTCGCACAGCGATTCAAGGATGTGATGGAGTGTTTCATGTTGCCAGTCCAGTTCCCTACGACCCTGTAGAATTGATTGAACCTGCCGTGAAGGGAACACTTAATGTACTTAAAGCATGCCTTGAAGCAAAAGTCAAGCGAGTTGTTTTCGTGTCAGCTGCAGCTTCTCTTGTCATGAATCCTAAGTGGTCTCAAGGCCAAGTGTTGGATGAAACTTGTTGGTCAGACAAGGAATACTGCAGAAGTACTAAGAATTGGTATTTTCTTTCAAAAACTGAAGCAGAATATGAAGCCCTGGAGTATGCAAGAATAAATGGGCTCGACTTGGTAACCGTTTGTCCTACACTTATTATGGGGCCAATTCTGCAGTCCACTGTAAATGCAAGTACCTTGGTCGTCATCAAGCTTCTGAAGGAAGGGTATGAGTCACTGGAAAACAAGCCGCGGATGATAGTTGATGTGCGGGATGTAGCTGAAGCAGTCGTTATGGTGAATGAGAAGCCTGAGGCTGAAGGAAGATACATATGCACCGCTCACAATATCAAGACGAGAGATCTGGTTGAAGAGCTGAAGAGCATTTATCCTAATTACAGTTATCCTATGAACTTTGTTGAAGTAGAAGAACAACCAAGGTTGAGCTCGGAGAAACTTCAGAGGCTGGGTTTGAGTTTCCGACCATTGAAAGAGACGCTTACTGGCTCAGTTGAAAGCTACAAAGAGGCTGGACTGCTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

288

Amino Acids

32.2

Weight (kDa)

5.59

Isoelectric Point (pI)

33.48

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 5 - 121 1.4e-07 NmrA-like family
GDP_Man_Dehyd PF16363 8 - 232 6.7e-09 GDP-mannose 4,6 dehydratase
Epimerase PF01370 20 - 214 9.9e-17 NAD dependent epimerase/dehydratase family
NAD_binding_10 PF13460 20 - 158 1.2e-10 NAD(P)H-binding
3Beta_HSD PF01073 24 - 173 6.5e-13 3-beta hydroxysteroid dehydrogenase/isomerase family
Polysacc_synt_2 PF02719 29 - 96 1.5e-06 Polysaccharide biosynthesis protein
NAD_binding_4 PF07993 39 - 170 3.8e-09 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 220
AccB7I CCANNNNNTGG 1 cut(s) 334
AccBSI CCGCTC 1 cut(s) 662
AccII CGCG 1 cut(s) 574
AciI CCGC 5 cut(s) 107, 572, 574, 592, 660
AcuI CTGAAG 6 cut(s) 426, 560, 624, 660, 719, 764
AfaI GTAC 4 cut(s) 13, 237, 376, 517
AfiI CCNNNNNNNGG 1 cut(s) 334
AjnI CCWGG 1 cut(s) 423
AluBI AGCT 8 cut(s) 41, 287, 293, 535, 602, 697, 768, 840
AluI AGCT 8 cut(s) 41, 287, 293, 535, 602, 697, 768, 840
Alw21I GWGCWC 2 cut(s) 32, 770
Alw26I GTCTC 2 cut(s) 324, 809
Alw44I GTGCAC 1 cut(s) 28
AlwNI CAGNNNCTG 1 cut(s) 787
AoxI GGCC 2 cut(s) 325, 485
ApaLI GTGCAC 1 cut(s) 28
ApeKI GCWGC 3 cut(s) 38, 287, 290
AseI ATTAAT 1 cut(s) 6
Asp700I GAANNNNTTC 1 cut(s) 129
AspS9I GGNCC 1 cut(s) 485
AsuHPI GGTGA 1 cut(s) 631
AxyI CCTNAGG 1 cut(s) 633
BaeGI GKGCMC 1 cut(s) 32
BanII GRGCYC 2 cut(s) 450, 770
Bbv12I GWGCWC 2 cut(s) 32, 770
BbvI GCAGC 3 cut(s) 50, 274, 302
BccI CCATC 1 cut(s) 149
BceAI ACGGC 1 cut(s) 200
BciT130I CCWGG 1 cut(s) 425
BcoDI GTCTC 2 cut(s) 324, 809
BfmI CTRYAG 4 cut(s) 195, 288, 367, 494
BfuAI ACCTGC 1 cut(s) 220
BglII AGATCT 1 cut(s) 682
BisI GCNGC 4 cut(s) 39, 288, 291, 572
BlsI GCNGC 4 cut(s) 40, 289, 292, 573
BmcAI AGTACT 2 cut(s) 13, 376
Bme1390I CCNGG 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 485
BmiI GGNNCC 1 cut(s) 486
BmrFI CCNGG 1 cut(s) 425
BmsI GCATC 1 cut(s) 89
BpmI CTGGAG 1 cut(s) 446
BpuEI CTTGAG 1 cut(s) 306
BsaI GGTCTC 1 cut(s) 324
BsaJI CCNNGG 4 cut(s) 423, 519, 572, 758
Bsc4I CCNNNNNNNGG 1 cut(s) 334
Bse1I ACTGG 4 cut(s) 174, 179, 564, 829
Bse21I CCTNAGG 1 cut(s) 633
BseBI CCWGG 1 cut(s) 425
BseDI CCNNGG 4 cut(s) 423, 519, 572, 758
BseGI GGATG 4 cut(s) 155, 343, 582, 601
BseLI CCNNNNNNNGG 1 cut(s) 334
BseMII CTCAG 3 cut(s) 75, 624, 843
BseNI ACTGG 4 cut(s) 174, 179, 564, 829
BseSI GKGCMC 1 cut(s) 32
BseXI GCAGC 3 cut(s) 50, 274, 302
BseYI CCCAGC 1 cut(s) 787
Bsh1236I CGCG 1 cut(s) 574
BshFI GGCC 2 cut(s) 327, 487
BsiHKAI GWGCWC 2 cut(s) 32, 770
BslI CCNNNNNNNGG 1 cut(s) 334
BsmAI GTCTC 2 cut(s) 324, 809
BsmBI CGTCTC 1 cut(s) 809
BsnI GGCC 2 cut(s) 327, 487
Bso31I GGTCTC 1 cut(s) 324
Bsp1286I GDGCHC 3 cut(s) 32, 450, 770
Bsp143I GATC 1 cut(s) 682
BspACI CCGC 5 cut(s) 107, 572, 574, 592, 660
BspANI GGCC 2 cut(s) 327, 487
BspCNI CTCAG 3 cut(s) 76, 625, 842
BspFNI CGCG 1 cut(s) 574
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 1 cut(s) 486
BspMAI CTGCAG 3 cut(s) 292, 371, 498
BspMI ACCTGC 1 cut(s) 220
BspQI GCTCTTC 2 cut(s) 687, 695
BspTNI GGTCTC 1 cut(s) 324
BsrBI CCGCTC 1 cut(s) 662
BsrI ACTGG 4 cut(s) 174, 179, 564, 829
BssECI CCNNGG 4 cut(s) 423, 519, 572, 758
BssMI GATC 1 cut(s) 682
BssT1I CCWWGG 2 cut(s) 519, 758
Bst2UI CCWGG 1 cut(s) 425
Bst4CI ACNGT 4 cut(s) 16, 463, 505, 722
Bst6I CTCTTC 2 cut(s) 687, 695
BstC8I GCNNGC 2 cut(s) 54, 247
BstDEI CTNAG 5 cut(s) 84, 312, 378, 633, 829
BstDSI CCRYGG 1 cut(s) 572
BstEII GGTNACC 1 cut(s) 457
BstF5I GGATG 4 cut(s) 155, 343, 582, 601
BstFNI CGCG 1 cut(s) 574
BstKTI GATC 1 cut(s) 685
BstMAI GTCTC 2 cut(s) 324, 809
BstMBI GATC 1 cut(s) 682
BstMWI GCNNNNNNNGC 1 cut(s) 493
BstNI CCWGG 1 cut(s) 425
BstNSI RCATGY 1 cut(s) 249
BstPI GGTNACC 1 cut(s) 457
BstSCI CCNGG 1 cut(s) 423
BstSFI CTRYAG 4 cut(s) 195, 288, 367, 494
BstSLI GKGCMC 1 cut(s) 32
BstUI CGCG 1 cut(s) 574
BstV1I GCAGC 3 cut(s) 50, 274, 302
BstX2I RGATCY 1 cut(s) 682
BstYI RGATCY 1 cut(s) 682
Bsu36I CCTNAGG 1 cut(s) 633
BsuRI GGCC 2 cut(s) 327, 487
BtgI CCRYGG 1 cut(s) 572
BtsCI GGATG 4 cut(s) 155, 343, 582, 601
BtsIMutI CAGTG 2 cut(s) 501, 557
BveI ACCTGC 1 cut(s) 220
Cac8I GCNNGC 2 cut(s) 54, 247
CaiI CAGNNNCTG 1 cut(s) 787
CciI TCATGA 1 cut(s) 303
Cfr13I GGNCC 1 cut(s) 485
Cfr42I CCGCGG 1 cut(s) 575
CseI GACGC 1 cut(s) 826
Csp6I GTAC 4 cut(s) 12, 236, 375, 516
CviAII CATG 3 cut(s) 167, 246, 304
CviQI GTAC 4 cut(s) 12, 236, 375, 516
DdeI CTNAG 5 cut(s) 84, 312, 378, 633, 829
DpnI GATC 1 cut(s) 684
DpnII GATC 1 cut(s) 682
Eam1104I CTCTTC 2 cut(s) 687, 695
EarI CTCTTC 2 cut(s) 687, 695
Ecl136II GAGCTC 1 cut(s) 768
Eco130I CCWWGG 2 cut(s) 519, 758
Eco24I GRGCYC 2 cut(s) 450, 770
Eco31I GGTCTC 1 cut(s) 324
Eco53kI GAGCTC 1 cut(s) 768
Eco57I CTGAAG 6 cut(s) 426, 560, 624, 660, 719, 764
Eco81I CCTNAGG 1 cut(s) 633
Eco91I GGTNACC 1 cut(s) 457
EcoICRI GAGCTC 1 cut(s) 768
EcoO65I GGTNACC 1 cut(s) 457
EcoRII CCWGG 1 cut(s) 423
EcoT14I CCWWGG 2 cut(s) 519, 758
EcoT38I GRGCYC 2 cut(s) 450, 770
ErhI CCWWGG 2 cut(s) 519, 758
Esp3I CGTCTC 1 cut(s) 809
FaeI CATG 3 cut(s) 170, 249, 307
FalI AAGNNNNNCTT 4 cut(s) 234, 266, 804, 836
FatI CATG 3 cut(s) 166, 245, 303
FauI CCCGC 1 cut(s) 585
FauNDI CATATG 1 cut(s) 653
Fnu4HI GCNGC 4 cut(s) 39, 288, 291, 572
FokI GGATG 4 cut(s) 162, 350, 589, 608
FriOI GRGCYC 2 cut(s) 450, 770
Fsp4HI GCNGC 4 cut(s) 39, 288, 291, 572
GluI GCNGC 4 cut(s) 39, 288, 291, 572
GsaI CCCAGC 1 cut(s) 791
GsuI CTGGAG 1 cut(s) 446
HaeIII GGCC 2 cut(s) 327, 487
HgaI GACGC 1 cut(s) 826
Hin1II CATG 3 cut(s) 170, 249, 307
HindIII AAGCTT 1 cut(s) 533
HinfI GANTC 4 cut(s) 125, 142, 307, 554
HphI GGTGA 1 cut(s) 631
Hpy166II GTNNAC 2 cut(s) 30, 501
Hpy188I TCNGA 7 cut(s) 85, 355, 540, 772, 783, 803, 863
Hpy188III TCNNGA 2 cut(s) 304, 673
Hpy8I GTNNAC 2 cut(s) 30, 501
HpyAV CCTTC 5 cut(s) 140, 214, 535, 539, 635
HpyCH4III ACNGT 4 cut(s) 16, 463, 505, 722
HpyCH4V TGCA 7 cut(s) 30, 290, 369, 434, 496, 512, 657
HpyF10VI GCNNNNNNNGC 1 cut(s) 493
HpyF3I CTNAG 5 cut(s) 84, 312, 378, 633, 829
Hsp92II CATG 3 cut(s) 170, 249, 307
KspI CCGCGG 1 cut(s) 575
Kzo9I GATC 1 cut(s) 682
LguI GCTCTTC 2 cut(s) 687, 695
Lsp1109I GCAGC 3 cut(s) 50, 274, 302
LweI GCATC 1 cut(s) 89
MaeIII GTNAC 2 cut(s) 457, 555
MalI GATC 1 cut(s) 684
MbiI CCGCTC 1 cut(s) 662
MboI GATC 1 cut(s) 682
MboII GAAGA 4 cut(s) 657, 704, 712, 761
MflI RGATCY 1 cut(s) 682
MhlI GDGCHC 3 cut(s) 32, 450, 770
MluCI AATT 4 cut(s) 200, 382, 489, 715
MlyI GAGTC 1 cut(s) 563
MmeI TCCRAC 3 cut(s) 96, 315, 826
MnlI CCTC 3 cut(s) 628, 777, 841
MroXI GAANNNNTTC 1 cut(s) 129
MseI TTAA 4 cut(s) 6, 93, 231, 240
MspA1I CMGCKG 2 cut(s) 287, 574
MspR9I CCNGG 1 cut(s) 425
MvaI CCWGG 1 cut(s) 425
MvnI CGCG 1 cut(s) 574
MwoI GCNNNNNNNGC 1 cut(s) 493
NdeI CATATG 1 cut(s) 653
NdeII GATC 1 cut(s) 682
NlaIII CATG 3 cut(s) 170, 249, 307
NlaIV GGNNCC 1 cut(s) 486
NmuCI GTSAC 1 cut(s) 555
NspI RCATGY 1 cut(s) 249
PaeI GCATGC 1 cut(s) 249
PagI TCATGA 1 cut(s) 303
PciSI GCTCTTC 2 cut(s) 687, 695
PdmI GAANNNNTTC 1 cut(s) 129
PfeI GAWTC 3 cut(s) 125, 142, 307
PflMI CCANNNNNTGG 1 cut(s) 334
PkrI GCNGC 4 cut(s) 40, 289, 292, 573
PleI GAGTC 1 cut(s) 562
PpsI GAGTC 1 cut(s) 562
PshBI ATTAAT 1 cut(s) 6
Psp124BI GAGCTC 1 cut(s) 770
Psp6I CCWGG 1 cut(s) 423
PspEI GGTNACC 1 cut(s) 457
PspFI CCCAGC 1 cut(s) 787
PspGI CCWGG 1 cut(s) 423
PspN4I GGNNCC 1 cut(s) 486
PspPI GGNCC 1 cut(s) 485
PstI CTGCAG 3 cut(s) 292, 371, 498
PstNI CAGNNNCTG 1 cut(s) 787
PsuI RGATCY 1 cut(s) 682
PvuII CAGCTG 1 cut(s) 287
RsaI GTAC 4 cut(s) 13, 237, 376, 517
RsaNI GTAC 4 cut(s) 12, 236, 375, 516
SacI GAGCTC 1 cut(s) 770
SacII CCGCGG 1 cut(s) 575
SapI GCTCTTC 2 cut(s) 687, 695
SaqAI TTAA 4 cut(s) 6, 93, 231, 240
SatI GCNGC 4 cut(s) 39, 288, 291, 572
Sau3AI GATC 1 cut(s) 682
Sau96I GGNCC 1 cut(s) 485
ScaI AGTACT 2 cut(s) 13, 376
SchI GAGTC 1 cut(s) 563
ScrFI CCNGG 1 cut(s) 425
SduI GDGCHC 3 cut(s) 32, 450, 770
SfaNI GCATC 1 cut(s) 89
SfcI CTRYAG 4 cut(s) 195, 288, 367, 494
Sfr303I CCGCGG 1 cut(s) 575
SgrBI CCGCGG 1 cut(s) 575
SmlI CTYRAG 1 cut(s) 321
SmoI CTYRAG 1 cut(s) 321
SphI GCATGC 1 cut(s) 249
Sse9I AATT 4 cut(s) 200, 382, 489, 715
SsiI CCGC 5 cut(s) 107, 572, 574, 592, 660
SstI GAGCTC 1 cut(s) 770
StyD4I CCNGG 1 cut(s) 423
StyI CCWWGG 2 cut(s) 519, 758
TaaI ACNGT 4 cut(s) 16, 463, 505, 722
TaqI TCGA 1 cut(s) 450
TasI AATT 4 cut(s) 200, 382, 489, 715
TatI WGTACW 3 cut(s) 11, 235, 374
TauI GCSGC 1 cut(s) 574
TfiI GAWTC 3 cut(s) 125, 142, 307
Tru1I TTAA 4 cut(s) 6, 93, 231, 240
Tru9I TTAA 4 cut(s) 6, 93, 231, 240
TscAI CASTG 2 cut(s) 508, 564
TseFI GTSAC 1 cut(s) 555
TseI GCWGC 3 cut(s) 38, 287, 290
Tsp45I GTSAC 1 cut(s) 555
TspDTI ATGAA 6 cut(s) 138, 155, 320, 354, 432, 746
TspRI CASTG 2 cut(s) 508, 564
Van91I CCANNNNNTGG 1 cut(s) 334
VneI GTGCAC 1 cut(s) 28
VspI ATTAAT 1 cut(s) 6
XceI RCATGY 1 cut(s) 249
XmnI GAANNNNTTC 1 cut(s) 129
ZrmI AGTACT 2 cut(s) 13, 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.