AT2G33600

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
2
OG0002886
Physical Location & Seq
Forward (+)
14226668 .. 14228742
2075 bp
Loading structure...
UTR
Exon/CDS
Intron
AT2G33600.1

Sequence Viewer

Length: 966 bp
ATGGCGGTTGTGCAGAAAGGAAAGGTTTGTGTAACCGGTGCCGGAGGTTTTTTGGGTTCTTGGGTCGTCAATCATCTCCTCTCTAGGGATTACTTCGTCCACGGCACTGTCAGAGACCCTGGTAATGAAAAGTATGCTCATTTGAAGAAGCTTGATAAAGCTGGTGACAAACTCAAGCTCTTCAAGGCTGATTTGCTCAACTACGGCTCTCTTCAATCTGCTATTGCTGGCTGCAGCGGCGTCTTCCATGTCGCTTGCCCTGTTCCATCTGCTTCAGTCCCTAATCCTGAGGTAGATTTGATCGCACCTGCAGTGGATGGTACGCTGAATGTGCTTAAGGCATGCGTTGAAGCAAAAGTTAAGCGTGTTGTGTATGTTTCCTCTGTATCTGCAGTTGCTATGAACCCTATGTGGTCAAAGAGTCAAGTGTTAGATGAGACCGCTTGGTCTGACCAAGATTATTGCAAAAAAACTGAGAATTGGTATTCTTTGTCAAAGACACGAGCAGAAAGTGAAGCTTTCGAATTTGCAAAACGAACCGGACTTGATCTCGTCAGTGTTTGCCCTACCCTTGTCTTGGGACCTGTATTGCAGCAGCACACAGTAAATGCTAGTAGCCTTGTTCTTCTCAAGCTTCTTAAAGAGGGTTATGAATCGCGTAATAACCAAGAACGGCATTTGGTAGATGTGCGTGATGTGGCTCAAGCTTTACTTTTGGTTTATGAGAAAGCAGAAGCTGAAGGAAGGTACATCTGCATAGGCCACACGGTTAGGGAACAAGAAGTCGCTGAGAAGCTGAAAAGTCTTTATCTAAATTACAACTACCCAAAGAGATACATCGAAGCGGATGGAAAAGTGAAGGTGAGTTCAGAGAAGTTGCAGAAGTTGGGTTGGACTTACCGGCCGTTGGAGGAAACACTTGTGGATTCTGTTGAGAGCTACCGCAAAGCTAAGCTTGTGGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

321

Amino Acids

35.61

Weight (kDa)

8.5

Isoelectric Point (pI)

25.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 6 - 133 9.6e-11 NmrA-like family
KR PF08659 7 - 136 1.2e-06 KR domain
Epimerase PF01370 9 - 250 5.7e-29 NAD dependent epimerase/dehydratase family
Polysacc_synt_2 PF02719 9 - 149 8.4e-09 Polysaccharide biosynthesis protein
3Beta_HSD PF01073 11 - 239 1.4e-21 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 11 - 305 4.1e-18 GDP-mannose 4,6 dehydratase
NAD_binding_4 PF07993 11 - 198 5e-11 Male sterility protein
NAD_binding_10 PF13460 13 - 189 1.3e-13 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 316
AasI GACNNNNNNGTC 1 cut(s) 445
Acc36I ACCTGC 1 cut(s) 316
AccB1I GGYRCC 1 cut(s) 38
AccII CGCG 1 cut(s) 658
AciI CCGC 5 cut(s) 5, 237, 441, 845, 943
AcoI YGGCCR 1 cut(s) 902
AcsI RAATTY 1 cut(s) 524
AcuI CTGAAG 2 cut(s) 258, 759
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 2 cut(s) 322, 749
AfiI CCNNNNNNNGG 3 cut(s) 85, 577, 907
AflII CTTAAG 1 cut(s) 335
AgeI ACCGGT 1 cut(s) 35
AgsI TTSAA 4 cut(s) 145, 184, 215, 350
AjnI CCWGG 1 cut(s) 118
Alw26I GTCTC 2 cut(s) 108, 431
AlwNI CAGNNNCTG 1 cut(s) 737
AoxI GGCC 2 cut(s) 760, 902
ApeKI GCWGC 4 cut(s) 231, 234, 592, 595
ApoI RAATTY 1 cut(s) 524
AsiGI ACCGGT 1 cut(s) 35
AspS9I GGNCC 1 cut(s) 581
AsuHPI GGTGA 2 cut(s) 176, 874
AsuII TTCGAA 1 cut(s) 522
AvaII GGWCC 1 cut(s) 581
AxyI CCTNAGG 1 cut(s) 288
BanI GGYRCC 1 cut(s) 38
BauI CACGAG 1 cut(s) 501
BbsI GAAGAC 1 cut(s) 235
BbvI GCAGC 4 cut(s) 218, 246, 604, 607
BccI CCATC 3 cut(s) 274, 311, 842
BceAI ACGGC 4 cut(s) 118, 220, 689, 889
BciT130I CCWGG 1 cut(s) 120
BcoDI GTCTC 2 cut(s) 108, 431
BfaI CTAG 2 cut(s) 84, 612
BfmI CTRYAG 3 cut(s) 232, 309, 390
BfrI CTTAAG 1 cut(s) 335
BfuAI ACCTGC 1 cut(s) 316
BisI GCNGC 5 cut(s) 232, 235, 238, 593, 596
BlpI GCTNAGC 1 cut(s) 951
BlsI GCNGC 5 cut(s) 233, 236, 239, 594, 597
Bme1390I CCNGG 1 cut(s) 120
Bme18I GGWCC 1 cut(s) 581
BmgT120I GGNCC 1 cut(s) 581
BmiI GGNNCC 2 cut(s) 40, 582
BmrFI CCNGG 1 cut(s) 120
BpiI GAAGAC 1 cut(s) 235
Bpu1102I GCTNAGC 1 cut(s) 951
Bpu14I TTCGAA 1 cut(s) 522
BpuEI CTTGAG 3 cut(s) 158, 614, 687
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 2 cut(s) 108, 431
BsaJI CCNNGG 2 cut(s) 100, 118
BsaWI WCCGGW 2 cut(s) 35, 539
Bsc4I CCNNNNNNNGG 3 cut(s) 85, 577, 907
Bse118I RCCGGY 2 cut(s) 35, 900
Bse21I CCTNAGG 1 cut(s) 288
BseBI CCWGG 1 cut(s) 120
BseDI CCNNGG 2 cut(s) 100, 118
BseGI GGATG 2 cut(s) 322, 853
BseLI CCNNNNNNNGG 3 cut(s) 85, 577, 907
BseMII CTCAG 3 cut(s) 279, 465, 780
BseRI GAGGAG 1 cut(s) 68
BseX3I CGGCCG 1 cut(s) 902
BseXI GCAGC 4 cut(s) 218, 246, 604, 607
BsgI GTGCAG 1 cut(s) 32
Bsh1236I CGCG 1 cut(s) 658
Bsh1285I CGRYCG 1 cut(s) 905
BshFI GGCC 2 cut(s) 762, 904
BshNI GGYRCC 1 cut(s) 38
BshTI ACCGGT 1 cut(s) 35
BsiEI CGRYCG 1 cut(s) 905
BsiSI CCGG 4 cut(s) 36, 42, 540, 901
BslFI GGGAC 2 cut(s) 263, 594
BslI CCNNNNNNNGG 3 cut(s) 85, 577, 907
BsmAI GTCTC 2 cut(s) 108, 431
BsmFI GGGAC 2 cut(s) 263, 594
BsnI GGCC 2 cut(s) 762, 904
Bso31I GGTCTC 2 cut(s) 108, 431
Bsp119I TTCGAA 1 cut(s) 522
Bsp143I GATC 2 cut(s) 300, 547
Bsp1720I GCTNAGC 1 cut(s) 951
BspACI CCGC 5 cut(s) 5, 237, 441, 845, 943
BspANI GGCC 2 cut(s) 762, 904
BspCNI CTCAG 3 cut(s) 280, 466, 781
BspFNI CGCG 1 cut(s) 658
BspLI GGNNCC 2 cut(s) 40, 582
BspMAI CTGCAG 3 cut(s) 236, 313, 394
BspMI ACCTGC 1 cut(s) 316
BspQI GCTCTTC 1 cut(s) 185
BspT104I TTCGAA 1 cut(s) 522
BspT107I GGYRCC 1 cut(s) 38
BspTI CTTAAG 1 cut(s) 335
BspTNI GGTCTC 2 cut(s) 108, 431
BsrFI RCCGGY 2 cut(s) 35, 900
BssAI RCCGGY 2 cut(s) 35, 900
BssECI CCNNGG 2 cut(s) 100, 118
BssMI GATC 2 cut(s) 300, 547
BssNI GRCGYC 1 cut(s) 240
BssSI CACGAG 1 cut(s) 501
Bst2BI CACGAG 1 cut(s) 501
Bst2UI CCWGG 1 cut(s) 120
Bst4CI ACNGT 3 cut(s) 109, 604, 769
Bst6I CTCTTC 2 cut(s) 185, 216
BstACI GRCGYC 1 cut(s) 240
BstAFI CTTAAG 1 cut(s) 335
BstBI TTCGAA 1 cut(s) 522
BstC8I GCNNGC 3 cut(s) 229, 256, 343
BstDEI CTNAG 4 cut(s) 288, 474, 789, 951
BstDSI CCRYGG 1 cut(s) 100
BstF5I GGATG 2 cut(s) 322, 853
BstFNI CGCG 1 cut(s) 658
BstKTI GATC 2 cut(s) 303, 550
BstMAI GTCTC 2 cut(s) 108, 431
BstMBI GATC 2 cut(s) 300, 547
BstMCI CGRYCG 1 cut(s) 905
BstMWI GCNNNNNNNGC 2 cut(s) 237, 331
BstNI CCWGG 1 cut(s) 120
BstNSI RCATGY 1 cut(s) 345
BstSCI CCNGG 1 cut(s) 118
BstSFI CTRYAG 3 cut(s) 232, 309, 390
BstUI CGCG 1 cut(s) 658
BstV1I GCAGC 4 cut(s) 218, 246, 604, 607
BstV2I GAAGAC 1 cut(s) 235
BstZI CGGCCG 1 cut(s) 902
Bsu36I CCTNAGG 1 cut(s) 288
BsuRI GGCC 2 cut(s) 762, 904
BtgI CCRYGG 1 cut(s) 100
BtsCI GGATG 2 cut(s) 322, 853
BtsI GCAGTG 1 cut(s) 318
BtsIMutI CAGTG 3 cut(s) 105, 318, 562
BveI ACCTGC 1 cut(s) 316
Cac8I GCNNGC 3 cut(s) 229, 256, 343
CaiI CAGNNNCTG 1 cut(s) 737
Cfr10I RCCGGY 2 cut(s) 35, 900
Cfr13I GGNCC 1 cut(s) 581
CseI GACGC 1 cut(s) 229
Csp6I GTAC 2 cut(s) 321, 748
CspAI ACCGGT 1 cut(s) 35
CviAII CATG 2 cut(s) 248, 342
CviQI GTAC 2 cut(s) 321, 748
DdeI CTNAG 4 cut(s) 288, 474, 789, 951
DpnI GATC 2 cut(s) 302, 549
DpnII GATC 2 cut(s) 300, 547
DrdI GACNNNNNNGTC 1 cut(s) 445
DseDI GACNNNNNNGTC 1 cut(s) 445
EaeI YGGCCR 1 cut(s) 902
EagI CGGCCG 1 cut(s) 902
Eam1104I CTCTTC 2 cut(s) 185, 216
EarI CTCTTC 2 cut(s) 185, 216
EclXI CGGCCG 1 cut(s) 902
Eco31I GGTCTC 2 cut(s) 108, 431
Eco47I GGWCC 1 cut(s) 581
Eco52I CGGCCG 1 cut(s) 902
Eco57I CTGAAG 2 cut(s) 258, 759
Eco81I CCTNAGG 1 cut(s) 288
EcoO109I RGGNCCY 1 cut(s) 581
EcoRII CCWGG 1 cut(s) 118
FaeI CATG 2 cut(s) 251, 345
FaiI YATR 9 cut(s) 135, 249, 343, 375, 401, 410, 651, 723, 758
FalI AAGNNNNNCTT 5 cut(s) 502, 534, 696, 728, 939
FaqI GGGAC 2 cut(s) 263, 594
FatI CATG 2 cut(s) 247, 341
Fnu4HI GCNGC 5 cut(s) 232, 235, 238, 593, 596
FokI GGATG 2 cut(s) 329, 860
Fsp4HI GCNGC 5 cut(s) 232, 235, 238, 593, 596
FspBI CTAG 2 cut(s) 84, 612
GluI GCNGC 5 cut(s) 232, 235, 238, 593, 596
HaeIII GGCC 2 cut(s) 762, 904
HapII CCGG 4 cut(s) 36, 42, 540, 901
HgaI GACGC 1 cut(s) 229
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 2 cut(s) 251, 345
HindIII AAGCTT 5 cut(s) 149, 516, 632, 705, 953
HinfI GANTC 3 cut(s) 421, 653, 926
HpaII CCGG 4 cut(s) 36, 42, 540, 901
HphI GGTGA 2 cut(s) 176, 874
Hpy166II GTNNAC 2 cut(s) 100, 961
Hpy188I TCNGA 3 cut(s) 113, 451, 871
Hpy188III TCNNGA 1 cut(s) 287
Hpy8I GTNNAC 2 cut(s) 100, 961
HpyAV CCTTC 3 cut(s) 734, 738, 853
HpyCH4III ACNGT 3 cut(s) 109, 604, 769
HpyCH4V TGCA 9 cut(s) 13, 234, 311, 392, 465, 530, 592, 756, 880
HpyF10VI GCNNNNNNNGC 2 cut(s) 237, 331
HpyF3I CTNAG 4 cut(s) 288, 474, 789, 951
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 2 cut(s) 251, 345
Kzo9I GATC 2 cut(s) 300, 547
LguI GCTCTTC 1 cut(s) 185
Lsp1109I GCAGC 4 cut(s) 218, 246, 604, 607
MaeI CTAG 2 cut(s) 84, 612
MaeIII GTNAC 2 cut(s) 31, 164
MalI GATC 2 cut(s) 302, 549
MboI GATC 2 cut(s) 300, 547
MboII GAAGA 5 cut(s) 157, 172, 203, 235, 617
MluCI AATT 3 cut(s) 478, 524, 814
MlyI GAGTC 1 cut(s) 430
MmeI TCCRAC 2 cut(s) 872, 888
MnlI CCTC 6 cut(s) 38, 89, 283, 391, 637, 904
MseI TTAA 3 cut(s) 336, 360, 639
MspA1I CMGCKG 1 cut(s) 237
MspCI CTTAAG 1 cut(s) 335
MspI CCGG 4 cut(s) 36, 42, 540, 901
MspR9I CCNGG 1 cut(s) 120
MvaI CCWGG 1 cut(s) 120
MvnI CGCG 1 cut(s) 658
MwoI GCNNNNNNNGC 2 cut(s) 237, 331
NdeII GATC 2 cut(s) 300, 547
NlaIII CATG 2 cut(s) 251, 345
NlaIV GGNNCC 2 cut(s) 40, 582
NmuCI GTSAC 1 cut(s) 164
NspI RCATGY 1 cut(s) 345
NspV TTCGAA 1 cut(s) 522
PaeI GCATGC 1 cut(s) 345
PaqCI CACCTGC 1 cut(s) 316
PciSI GCTCTTC 1 cut(s) 185
PfeI GAWTC 2 cut(s) 653, 926
PinAI ACCGGT 1 cut(s) 35
PkrI GCNGC 5 cut(s) 233, 236, 239, 594, 597
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
PpuMI RGGWCCY 1 cut(s) 581
Psp5II RGGWCCY 1 cut(s) 581
Psp6I CCWGG 1 cut(s) 118
PspGI CCWGG 1 cut(s) 118
PspN4I GGNNCC 2 cut(s) 40, 582
PspPI GGNCC 1 cut(s) 581
PspPPI RGGWCCY 1 cut(s) 581
PstI CTGCAG 3 cut(s) 236, 313, 394
PstNI CAGNNNCTG 1 cut(s) 737
RsaI GTAC 2 cut(s) 322, 749
RsaNI GTAC 2 cut(s) 321, 748
SapI GCTCTTC 1 cut(s) 185
SaqAI TTAA 3 cut(s) 336, 360, 639
SatI GCNGC 5 cut(s) 232, 235, 238, 593, 596
Sau3AI GATC 2 cut(s) 300, 547
Sau96I GGNCC 1 cut(s) 581
SchI GAGTC 1 cut(s) 430
ScrFI CCNGG 1 cut(s) 120
SfcI CTRYAG 3 cut(s) 232, 309, 390
SfuI TTCGAA 1 cut(s) 522
SinI GGWCC 1 cut(s) 581
SmlI CTYRAG 4 cut(s) 173, 335, 629, 702
SmoI CTYRAG 4 cut(s) 173, 335, 629, 702
SphI GCATGC 1 cut(s) 345
Sse9I AATT 3 cut(s) 478, 524, 814
SsiI CCGC 5 cut(s) 5, 237, 441, 845, 943
SspMI CTAG 2 cut(s) 84, 612
StyD4I CCNGG 1 cut(s) 118
TaaI ACNGT 3 cut(s) 109, 604, 769
TaqI TCGA 2 cut(s) 522, 840
TasI AATT 3 cut(s) 478, 524, 814
TauI GCSGC 1 cut(s) 240
TfiI GAWTC 2 cut(s) 653, 926
Tru1I TTAA 3 cut(s) 336, 360, 639
Tru9I TTAA 3 cut(s) 336, 360, 639
TscAI CASTG 3 cut(s) 112, 318, 562
TseFI GTSAC 1 cut(s) 164
TseI GCWGC 4 cut(s) 231, 234, 592, 595
Tsp45I GTSAC 1 cut(s) 164
TspDTI ATGAA 3 cut(s) 141, 416, 666
TspRI CASTG 3 cut(s) 112, 318, 562
Vha464I CTTAAG 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 581
XapI RAATTY 1 cut(s) 524
XceI RCATGY 1 cut(s) 345
XspI CTAG 2 cut(s) 84, 612
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.