Rh7AG302600

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0002886
Physical Location & Seq
Forward (+)
33268141 .. 33270969
2829 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG302600.1

Sequence Viewer

Length: 843 bp
ATGGCAGTTCAGAATGGAAGGTTCTGTGTTACAGGGGCTGGAGGCTTCGTTGGCTCCTGGGTCGTTAAGCTTCTTCTTTCCAAGAACTGCATAGTCCACGGAACCGTCAGAGAACCCACGGATGACAAGAACTCTCACTTGCGAAAGCTTGACAAGGCATCTGAGAACCTGAAACTGTTCAAGGCGGACTTATTGGATTATGACTCCCTTCGCACGGCAATTGAAGGATGTGATGGAGTATTTCATGTTGCCAGTCCCGTTCCCTCCGGCAGTGTGCCAAATCCTGAGGCAAGTGTCTCTCATTATCCTCCAATATTGGTACTTGCACTGGTTAACATGCTCAATCGTGTTTCCCAGAACTGGTATTGCCTTTCAAAAACTGAAGCAGAATCTGAGGCTCTGCAGTTTGCAAAAACAACCGGGTTTGATCTTGTGACTGTTTGTCCTACACTTATACTTGGCCCAATTCTGCAGTTGACTGTAAATGCAAGTACCTTGGTCCTCATCAAGCTTTTGAAGGAAGGAAAGGAGTCACTGGAAAACAGGCCCCGGAAGATAGTTGATGTACGGGATTTTGCTGAAGCACTTCTTATGGCATATGAGAAGCCGGAGGCTGAAGGAAGATACATATGCATGTCCCACAATGTTAAGAATAGGGATCTAGTTGAGAAGCTAAGGCGCCTTTATCGGAATTACAATTATCCGAAGAACTTTGTTGAAGTAGGCGAAGACAGACAGGTGAGCTCAGAGAAACTGCAAAGGCTAGGTTGGAGTTACCGGTCATTGGATGATACTCTCATTGACTCTGTTGAAAGCTACCGGGAGGCTGGACTATTGGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

280

Amino Acids

31.38

Weight (kDa)

6.54

Isoelectric Point (pI)

35.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 84 3.8e-07 NmrA-like family
Epimerase PF01370 9 - 88 3.7e-11 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 10 - 98 3.7e-12 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 10 - 92 4.7e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_10 PF13460 12 - 85 4.3e-08 NAD(P)H-binding
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 678
AciI CCGC 1 cut(s) 185
AclWI GGATC 1 cut(s) 666
AcuI CTGAAG 3 cut(s) 402, 600, 636
AcyI GRCGYC 1 cut(s) 679
AfaI GTAC 3 cut(s) 321, 493, 567
AfiI CCNNNNNNNGG 3 cut(s) 214, 360, 784
AgeI ACCGGT 1 cut(s) 777
AgsI TTSAA 6 cut(s) 181, 224, 375, 517, 719, 812
AhdI GACNNNNNGTC 1 cut(s) 441
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 6 cut(s) 70, 148, 511, 673, 744, 816
AluI AGCT 6 cut(s) 70, 148, 511, 673, 744, 816
Alw21I GWGCWC 1 cut(s) 746
Alw26I GTCTC 1 cut(s) 301
AlwI GGATC 1 cut(s) 666
AlwNI CAGNNNCTG 2 cut(s) 38, 392
AoxI GGCC 2 cut(s) 460, 545
AsiGI ACCGGT 1 cut(s) 777
Asp700I GAANNNNTTC 2 cut(s) 176, 585
AspLEI GCGC 1 cut(s) 681
AspS9I GGNCC 3 cut(s) 461, 499, 546
AsuC2I CCSGG 3 cut(s) 421, 550, 821
AsuHPI GGTGA 1 cut(s) 751
AvaII GGWCC 1 cut(s) 499
AxyI CCTNAGG 1 cut(s) 285
BanI GGYRCC 1 cut(s) 678
BanII GRGCYC 1 cut(s) 746
BbsI GAAGAC 1 cut(s) 735
Bbv12I GWGCWC 1 cut(s) 746
BccI CCATC 1 cut(s) 227
BceAI ACGGC 1 cut(s) 231
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 3 cut(s) 421, 550, 821
BcoDI GTCTC 1 cut(s) 301
BfaI CTAG 2 cut(s) 662, 764
BfmI CTRYAG 2 cut(s) 401, 470
BfoI RGCGCY 1 cut(s) 682
Bme1390I CCNGG 4 cut(s) 58, 421, 550, 821
Bme18I GGWCC 1 cut(s) 499
BmeRI GACNNNNNGTC 1 cut(s) 441
BmgT120I GGNCC 3 cut(s) 461, 499, 546
BmiI GGNNCC 4 cut(s) 55, 103, 548, 680
BmrFI CCNGG 4 cut(s) 58, 421, 550, 821
BmsI GCATC 1 cut(s) 167
BpiI GAAGAC 1 cut(s) 735
BpmI CTGGAG 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 674
BpuMI CCSGG 3 cut(s) 421, 550, 821
BsaHI GRCGYC 1 cut(s) 679
BsaJI CCNNGG 5 cut(s) 57, 97, 117, 495, 548
BsaWI WCCGGW 1 cut(s) 777
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 3 cut(s) 214, 360, 784
Bse118I RCCGGY 1 cut(s) 777
Bse1I ACTGG 4 cut(s) 252, 333, 365, 540
Bse21I CCTNAGG 1 cut(s) 285
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 5 cut(s) 57, 97, 117, 495, 548
BseGI GGATG 3 cut(s) 127, 233, 793
BseLI CCNNNNNNNGG 3 cut(s) 214, 360, 784
BseMII CTCAG 4 cut(s) 153, 276, 384, 759
BseNI ACTGG 4 cut(s) 252, 333, 365, 540
BshFI GGCC 2 cut(s) 462, 547
BshNI GGYRCC 1 cut(s) 678
BshTI ACCGGT 1 cut(s) 777
BsiHKAI GWGCWC 1 cut(s) 746
BsiSI CCGG 6 cut(s) 267, 420, 550, 608, 778, 820
BslFI GGGAC 2 cut(s) 240, 622
BslI CCNNNNNNNGG 3 cut(s) 214, 360, 784
BsmAI GTCTC 1 cut(s) 301
BsmFI GGGAC 2 cut(s) 240, 622
BsnI GGCC 2 cut(s) 462, 547
Bsp1286I GDGCHC 1 cut(s) 746
Bsp143I GATC 2 cut(s) 427, 658
BspACI CCGC 1 cut(s) 185
BspANI GGCC 2 cut(s) 462, 547
BspCNI CTCAG 4 cut(s) 154, 277, 385, 758
BspLI GGNNCC 4 cut(s) 55, 103, 548, 680
BspMAI CTGCAG 2 cut(s) 405, 474
BspPI GGATC 1 cut(s) 666
BspT107I GGYRCC 1 cut(s) 678
BsrFI RCCGGY 1 cut(s) 777
BsrI ACTGG 4 cut(s) 252, 333, 365, 540
BssAI RCCGGY 1 cut(s) 777
BssECI CCNNGG 5 cut(s) 57, 97, 117, 495, 548
BssMI GATC 2 cut(s) 427, 658
BssNI GRCGYC 1 cut(s) 679
BssT1I CCWWGG 1 cut(s) 495
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 4 cut(s) 106, 177, 439, 481
BstACI GRCGYC 1 cut(s) 679
BstDEI CTNAG 5 cut(s) 162, 285, 393, 674, 745
BstDSI CCRYGG 2 cut(s) 97, 117
BstF5I GGATG 3 cut(s) 127, 233, 793
BstH2I RGCGCY 1 cut(s) 682
BstHHI GCGC 1 cut(s) 681
BstKTI GATC 2 cut(s) 430, 661
BstMAI GTCTC 1 cut(s) 301
BstMBI GATC 2 cut(s) 427, 658
BstMWI GCNNNNNNNGC 1 cut(s) 51
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 2 cut(s) 340, 637
BstSCI CCNGG 4 cut(s) 56, 419, 548, 819
BstSFI CTRYAG 2 cut(s) 401, 470
BstV2I GAAGAC 1 cut(s) 735
BstX2I RGATCY 1 cut(s) 658
BstYI RGATCY 1 cut(s) 658
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 2 cut(s) 462, 547
BtgI CCRYGG 2 cut(s) 97, 117
BtsCI GGATG 3 cut(s) 127, 233, 793
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 3 cut(s) 277, 326, 533
CaiI CAGNNNCTG 2 cut(s) 38, 392
CfoI GCGC 1 cut(s) 681
Cfr10I RCCGGY 1 cut(s) 777
Cfr13I GGNCC 3 cut(s) 461, 499, 546
Csp6I GTAC 3 cut(s) 320, 492, 566
CspAI ACCGGT 1 cut(s) 777
CviAII CATG 3 cut(s) 245, 337, 634
CviQI GTAC 3 cut(s) 320, 492, 566
DdeI CTNAG 5 cut(s) 162, 285, 393, 674, 745
DinI GGCGCC 1 cut(s) 680
DpnI GATC 2 cut(s) 429, 660
DpnII GATC 2 cut(s) 427, 658
DriI GACNNNNNGTC 1 cut(s) 441
Eam1105I GACNNNNNGTC 1 cut(s) 441
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 744
Eco130I CCWWGG 1 cut(s) 495
Eco24I GRGCYC 1 cut(s) 746
Eco47I GGWCC 1 cut(s) 499
Eco53kI GAGCTC 1 cut(s) 744
Eco57I CTGAAG 3 cut(s) 402, 600, 636
Eco81I CCTNAGG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 744
EcoO109I RGGNCCY 1 cut(s) 546
EcoRII CCWGG 1 cut(s) 56
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 1 cut(s) 635
EcoT38I GRGCYC 1 cut(s) 746
EgeI GGCGCC 1 cut(s) 680
EheI GGCGCC 1 cut(s) 680
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 3 cut(s) 248, 340, 637
FalI AAGNNNNNCTT 4 cut(s) 173, 205, 573, 605
FaqI GGGAC 2 cut(s) 240, 622
FatI CATG 3 cut(s) 244, 336, 633
FauNDI CATATG 2 cut(s) 598, 629
FokI GGATG 3 cut(s) 134, 240, 800
FriOI GRGCYC 1 cut(s) 746
FspBI CTAG 2 cut(s) 662, 764
GlaI GCGC 1 cut(s) 680
GsuI CTGGAG 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 682
HaeIII GGCC 2 cut(s) 462, 547
HapII CCGG 6 cut(s) 267, 420, 550, 608, 778, 820
HhaI GCGC 1 cut(s) 681
Hin1I GRCGYC 1 cut(s) 679
Hin1II CATG 3 cut(s) 248, 340, 637
Hin6I GCGC 1 cut(s) 679
HinP1I GCGC 1 cut(s) 679
HincII GTYRAC 2 cut(s) 334, 477
HindII GTYRAC 2 cut(s) 334, 477
HindIII AAGCTT 3 cut(s) 68, 146, 509
HinfI GANTC 4 cut(s) 203, 389, 530, 803
HpaI GTTAAC 1 cut(s) 334
HpaII CCGG 6 cut(s) 267, 420, 550, 608, 778, 820
HphI GGTGA 1 cut(s) 751
Hpy166II GTNNAC 3 cut(s) 97, 334, 477
Hpy188I TCNGA 7 cut(s) 12, 110, 163, 394, 690, 705, 748
Hpy188III TCNNGA 1 cut(s) 284
Hpy8I GTNNAC 3 cut(s) 97, 334, 477
HpyAV CCTTC 6 cut(s) 12, 218, 218, 511, 515, 611
HpyCH4III ACNGT 4 cut(s) 106, 177, 439, 481
HpyCH4V TGCA 8 cut(s) 90, 326, 403, 410, 472, 488, 633, 757
HpyF10VI GCNNNNNNNGC 1 cut(s) 51
HpyF3I CTNAG 5 cut(s) 162, 285, 393, 674, 745
Hsp92I GRCGYC 1 cut(s) 679
Hsp92II CATG 3 cut(s) 248, 340, 637
HspAI GCGC 1 cut(s) 679
KasI GGCGCC 1 cut(s) 678
KspAI GTTAAC 1 cut(s) 334
Kzo9I GATC 2 cut(s) 427, 658
LmnI GCTCC 1 cut(s) 59
LweI GCATC 1 cut(s) 167
MaeI CTAG 2 cut(s) 662, 764
MaeIII GTNAC 4 cut(s) 28, 433, 531, 773
MalI GATC 2 cut(s) 429, 660
MboI GATC 2 cut(s) 427, 658
MboII GAAGA 5 cut(s) 65, 565, 633, 718, 740
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 658
MhlI GDGCHC 1 cut(s) 746
MluCI AATT 4 cut(s) 219, 465, 691, 697
Mly113I GGCGCC 1 cut(s) 679
MlyI GAGTC 3 cut(s) 197, 539, 797
MmeI TCCRAC 1 cut(s) 749
MnlI CCTC 8 cut(s) 35, 274, 280, 318, 388, 512, 604, 817
Mph1103I ATGCAT 1 cut(s) 635
MroXI GAANNNNTTC 2 cut(s) 176, 585
MseI TTAA 3 cut(s) 66, 333, 648
MslI CAYNNNNRTG 1 cut(s) 632
MspI CCGG 6 cut(s) 267, 420, 550, 608, 778, 820
MspR9I CCNGG 4 cut(s) 58, 421, 550, 821
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 1 cut(s) 51
NarI GGCGCC 1 cut(s) 679
NciI CCSGG 3 cut(s) 421, 550, 821
NdeI CATATG 2 cut(s) 598, 629
NdeII GATC 2 cut(s) 427, 658
NlaIII CATG 3 cut(s) 248, 340, 637
NlaIV GGNNCC 4 cut(s) 55, 103, 548, 680
NmuCI GTSAC 2 cut(s) 433, 531
NsiI ATGCAT 1 cut(s) 635
NspI RCATGY 2 cut(s) 340, 637
PdmI GAANNNNTTC 2 cut(s) 176, 585
PfeI GAWTC 1 cut(s) 389
PinAI ACCGGT 1 cut(s) 777
PleI GAGTC 3 cut(s) 197, 538, 797
PluTI GGCGCC 1 cut(s) 682
PpsI GAGTC 3 cut(s) 197, 538, 797
Psp124BI GAGCTC 1 cut(s) 746
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspN4I GGNNCC 4 cut(s) 55, 103, 548, 680
PspPI GGNCC 3 cut(s) 461, 499, 546
PstI CTGCAG 2 cut(s) 405, 474
PstNI CAGNNNCTG 2 cut(s) 38, 392
PsuI RGATCY 1 cut(s) 658
RsaI GTAC 3 cut(s) 321, 493, 567
RsaNI GTAC 3 cut(s) 320, 492, 566
RseI CAYNNNNRTG 1 cut(s) 632
SacI GAGCTC 1 cut(s) 746
SaqAI TTAA 3 cut(s) 66, 333, 648
Sau3AI GATC 2 cut(s) 427, 658
Sau96I GGNCC 3 cut(s) 461, 499, 546
SchI GAGTC 3 cut(s) 197, 539, 797
ScrFI CCNGG 4 cut(s) 58, 421, 550, 821
SduI GDGCHC 1 cut(s) 746
SfaNI GCATC 1 cut(s) 167
SfcI CTRYAG 2 cut(s) 401, 470
SfoI GGCGCC 1 cut(s) 680
SinI GGWCC 1 cut(s) 499
SmiMI CAYNNNNRTG 1 cut(s) 632
Sse9I AATT 4 cut(s) 219, 465, 691, 697
SsiI CCGC 1 cut(s) 185
SspDI GGCGCC 1 cut(s) 678
SspI AATATT 1 cut(s) 315
SspMI CTAG 2 cut(s) 662, 764
SstI GAGCTC 1 cut(s) 746
StyD4I CCNGG 4 cut(s) 56, 419, 548, 819
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 4 cut(s) 106, 177, 439, 481
TasI AATT 4 cut(s) 219, 465, 691, 697
TfiI GAWTC 1 cut(s) 389
Tru1I TTAA 3 cut(s) 66, 333, 648
Tru9I TTAA 3 cut(s) 66, 333, 648
TscAI CASTG 3 cut(s) 277, 333, 540
TseFI GTSAC 2 cut(s) 433, 531
Tsp45I GTSAC 2 cut(s) 433, 531
TspDTI ATGAA 1 cut(s) 233
TspGWI ACGGA 2 cut(s) 114, 134
TspRI CASTG 3 cut(s) 277, 333, 540
VpaK11BI GGWCC 1 cut(s) 499
XceI RCATGY 2 cut(s) 340, 637
XmnI GAANNNNTTC 2 cut(s) 176, 585
XspI CTAG 2 cut(s) 662, 764
Zsp2I ATGCAT 1 cut(s) 635
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.