Rroxscaffold_3G00242940

Cinnamoyl-CoA reductase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0002886
Physical Location & Seq
Forward (+)
35470522 .. 35473505
2984 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00242940.1

Sequence Viewer

Length: 1020 bp
ATGGTAGTTCAGAATGGAAGGTTCTGTGTAACGGGGGCTGGAGGCTTCGTTGGCTCCTGGGTCGTTAAGCTTCTTCTTTCCAAGAACTGCATAGTCCACGGAACCGTCAGAGAACCCACGGATGACAAGAACTCTCACTTGAGAAAGCTTGACAAGGCATCTGAGAACCTGAAACTGTTCAAGGCGGACTTATTGGATTATGACTCCCTTCGCTCGGCAATTGAAGGATGTGATGGAGTATTTCATGTTGCCAGTCCCGTTCCCTCCGGCAGTGTGCCAAATCCTGAGGCAAGTGTGTCTCTCATTATCCTCCAATATTGGTACTTGCACGAGTTAACATGCTCAATCGTAGAGTTAATTGAACCTGCTGTTAAGGGGACACTTAATGTACTTAAAGCATCTCTTGAAGCAAAAGTCAAGCGAGTTGTTTATGTGTCCTCTGTTGCTGCTGTGGCCATGAATCCTACGTGGGCTGAAGGTCAAGTATTGGATGAGTCTTGCTGGTCCGATAAGGAATACTGCAGAAATACTGAGAACTGGTATTGCCTTTCAAAAACTGAAGCAGAATCTGAGGCTCTGCAATTTGCAAAAACAACCGGGATTGATCTTGTGACTGTTTGTCCCTATCTTATACTCGGCCCAATTCTGCAGTCGACAGTAAATGCAAGTACCATGATCCTCATCAAGCTTTTGAAGGAAGGAAAGGAGTCACTGGAAAACAGGCCCCGGCAGATAGTTGACGTACGGGATTTAGCCGAAGCACTTCTTATGGCATATGAGAAGCCGGAGGCTGAAGGAAGATACATATGCAAGTCCCACAATGTTAAGAATGGGGATCTAGTTGAGAAGCTGAGGCGCCTTTATCCTAATTACAATTATCCGAAGAACTTTGTTGAAGTAGACGAAGACATAAAGGTGAGCTCAGCGAAACTGCAAAGGCTAGGTTGGAGTTACCGGGCATTGGATGAGACTCTCATCGACTCTGTTGAAAGCTACCGGGAAGCTGGACTATTACATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

339

Amino Acids

37.85

Weight (kDa)

5.67

Isoelectric Point (pI)

31.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NmrA PF05368 7 - 84 4.6e-07 NmrA-like family
Epimerase PF01370 9 - 88 5.5e-11 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 10 - 95 4.8e-08 3-beta hydroxysteroid dehydrogenase/isomerase family
GDP_Man_Dehyd PF16363 10 - 86 7.2e-12 GDP-mannose 4,6 dehydratase
NAD_binding_10 PF13460 12 - 152 1.9e-12 NAD(P)H-binding
Epimerase PF01370 118 - 265 5.8e-13 NAD dependent epimerase/dehydratase family
3Beta_HSD PF01073 123 - 225 1.5e-07 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 373
AccB1I GGYRCC 1 cut(s) 855
AccI GTMKAC 2 cut(s) 653, 900
AciI CCGC 1 cut(s) 185
AclWI GGATC 2 cut(s) 670, 843
AcoI YGGCCR 1 cut(s) 453
AcuI CTGAAG 3 cut(s) 495, 579, 813
AcyI GRCGYC 1 cut(s) 856
AfaI GTAC 4 cut(s) 323, 390, 670, 744
AfiI CCNNNNNNNGG 2 cut(s) 214, 961
AgsI TTSAA 8 cut(s) 181, 224, 362, 407, 552, 694, 896, 989
AhdI GACNNNNNGTC 1 cut(s) 618
AjnI CCWGG 1 cut(s) 56
AluBI AGCT 7 cut(s) 70, 148, 688, 850, 921, 993, 1004
AluI AGCT 7 cut(s) 70, 148, 688, 850, 921, 993, 1004
Alw21I GWGCWC 1 cut(s) 923
Alw26I GTCTC 2 cut(s) 303, 962
AlwI GGATC 2 cut(s) 670, 843
AlwNI CAGNNNCTG 1 cut(s) 569
AoxI GGCC 3 cut(s) 453, 637, 722
ApeKI GCWGC 1 cut(s) 446
Asp700I GAANNNNTTC 2 cut(s) 176, 762
AspLEI GCGC 1 cut(s) 858
AspS9I GGNCC 3 cut(s) 504, 638, 723
AsuC2I CCSGG 4 cut(s) 598, 727, 956, 998
AsuHPI GGTGA 1 cut(s) 928
AvaII GGWCC 1 cut(s) 504
AxyI CCTNAGG 1 cut(s) 285
BalI TGGCCA 1 cut(s) 455
BanI GGYRCC 1 cut(s) 855
BanII GRGCYC 1 cut(s) 923
BauI CACGAG 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 912
Bbv12I GWGCWC 1 cut(s) 923
BbvCI CCTCAGC 1 cut(s) 851
BbvI GCAGC 1 cut(s) 433
BccI CCATC 1 cut(s) 227
BciT130I CCWGG 1 cut(s) 58
BcnI CCSGG 4 cut(s) 598, 727, 956, 998
BcoDI GTCTC 2 cut(s) 303, 962
BfaI CTAG 2 cut(s) 839, 941
BfmI CTRYAG 2 cut(s) 520, 647
BfoI RGCGCY 1 cut(s) 859
BfuAI ACCTGC 1 cut(s) 373
BisI GCNGC 1 cut(s) 447
BlpI GCTNAGC 1 cut(s) 922
BlsI GCNGC 1 cut(s) 448
Bme1390I CCNGG 5 cut(s) 58, 598, 727, 956, 998
Bme18I GGWCC 1 cut(s) 504
BmeRI GACNNNNNGTC 1 cut(s) 618
BmgT120I GGNCC 3 cut(s) 504, 638, 723
BmiI GGNNCC 4 cut(s) 55, 103, 725, 857
BmrFI CCNGG 5 cut(s) 58, 598, 727, 956, 998
BmsI GCATC 2 cut(s) 167, 407
BpiI GAAGAC 1 cut(s) 912
BpmI CTGGAG 1 cut(s) 60
Bpu10I CCTNAGC 1 cut(s) 851
Bpu1102I GCTNAGC 1 cut(s) 922
BpuEI CTTGAG 1 cut(s) 160
BpuMI CCSGG 4 cut(s) 598, 727, 956, 998
BsaAI YACGTR 1 cut(s) 468
BsaBI GATNNNNATC 1 cut(s) 680
BsaHI GRCGYC 1 cut(s) 856
BsaJI CCNNGG 4 cut(s) 57, 97, 117, 725
BsaXI ACNNNNNCTCC 2 cut(s) 33, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 214, 961
Bse1I ACTGG 3 cut(s) 252, 542, 717
Bse21I CCTNAGG 1 cut(s) 285
Bse8I GATNNNNATC 1 cut(s) 680
BseBI CCWGG 1 cut(s) 58
BseDI CCNNGG 4 cut(s) 57, 97, 117, 725
BseGI GGATG 4 cut(s) 127, 233, 496, 970
BseJI GATNNNNATC 1 cut(s) 680
BseLI CCNNNNNNNGG 2 cut(s) 214, 961
BseMII CTCAG 6 cut(s) 153, 276, 522, 561, 842, 936
BseNI ACTGG 3 cut(s) 252, 542, 717
BseXI GCAGC 1 cut(s) 433
BshFI GGCC 3 cut(s) 455, 639, 724
BshNI GGYRCC 1 cut(s) 855
BsiHKAI GWGCWC 1 cut(s) 923
BsiSI CCGG 6 cut(s) 267, 597, 727, 785, 955, 997
BsiWI CGTACG 1 cut(s) 742
BslFI GGGAC 4 cut(s) 240, 391, 606, 799
BslI CCNNNNNNNGG 2 cut(s) 214, 961
BsmAI GTCTC 2 cut(s) 303, 962
BsmFI GGGAC 4 cut(s) 240, 391, 606, 799
BsnI GGCC 3 cut(s) 455, 639, 724
Bsp1286I GDGCHC 1 cut(s) 923
Bsp143I GATC 3 cut(s) 604, 675, 835
Bsp1720I GCTNAGC 1 cut(s) 922
BspACI CCGC 1 cut(s) 185
BspANI GGCC 3 cut(s) 455, 639, 724
BspCNI CTCAG 6 cut(s) 154, 277, 523, 562, 843, 935
BspLI GGNNCC 4 cut(s) 55, 103, 725, 857
BspMAI CTGCAG 2 cut(s) 524, 651
BspMI ACCTGC 1 cut(s) 373
BspPI GGATC 2 cut(s) 670, 843
BspT107I GGYRCC 1 cut(s) 855
BsrI ACTGG 3 cut(s) 252, 542, 717
BssECI CCNNGG 4 cut(s) 57, 97, 117, 725
BssMI GATC 3 cut(s) 604, 675, 835
BssNI GRCGYC 1 cut(s) 856
BssSI CACGAG 1 cut(s) 329
Bst2BI CACGAG 1 cut(s) 329
Bst2UI CCWGG 1 cut(s) 58
Bst4CI ACNGT 4 cut(s) 106, 177, 616, 658
BstACI GRCGYC 1 cut(s) 856
BstBAI YACGTR 1 cut(s) 468
BstDEI CTNAG 6 cut(s) 162, 285, 531, 570, 851, 922
BstDSI CCRYGG 2 cut(s) 97, 117
BstF5I GGATG 4 cut(s) 127, 233, 496, 970
BstH2I RGCGCY 1 cut(s) 859
BstHHI GCGC 1 cut(s) 858
BstKTI GATC 3 cut(s) 607, 678, 838
BstMAI GTCTC 2 cut(s) 303, 962
BstMBI GATC 3 cut(s) 604, 675, 835
BstMWI GCNNNNNNNGC 2 cut(s) 51, 452
BstNI CCWGG 1 cut(s) 58
BstNSI RCATGY 1 cut(s) 342
BstSCI CCNGG 5 cut(s) 56, 596, 725, 954, 996
BstSFI CTRYAG 2 cut(s) 520, 647
BstV1I GCAGC 1 cut(s) 433
BstV2I GAAGAC 1 cut(s) 912
BstX2I RGATCY 1 cut(s) 835
BstYI RGATCY 1 cut(s) 835
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 3 cut(s) 455, 639, 724
BtgI CCRYGG 2 cut(s) 97, 117
BtsCI GGATG 4 cut(s) 127, 233, 496, 970
BtsI GCAGTG 1 cut(s) 277
BtsIMutI CAGTG 2 cut(s) 277, 710
BveI ACCTGC 1 cut(s) 373
CaiI CAGNNNCTG 1 cut(s) 569
CfoI GCGC 1 cut(s) 858
Cfr13I GGNCC 3 cut(s) 504, 638, 723
Csp6I GTAC 4 cut(s) 322, 389, 669, 743
CviAII CATG 4 cut(s) 245, 339, 457, 673
CviQI GTAC 4 cut(s) 322, 389, 669, 743
DdeI CTNAG 6 cut(s) 162, 285, 531, 570, 851, 922
DinI GGCGCC 1 cut(s) 857
DpnI GATC 3 cut(s) 606, 677, 837
DpnII GATC 3 cut(s) 604, 675, 835
DriI GACNNNNNGTC 1 cut(s) 618
EaeI YGGCCR 1 cut(s) 453
Eam1105I GACNNNNNGTC 1 cut(s) 618
EciI GGCGGA 1 cut(s) 200
Ecl136II GAGCTC 1 cut(s) 921
Eco24I GRGCYC 1 cut(s) 923
Eco47I GGWCC 1 cut(s) 504
Eco53kI GAGCTC 1 cut(s) 921
Eco57I CTGAAG 3 cut(s) 495, 579, 813
Eco81I CCTNAGG 1 cut(s) 285
EcoICRI GAGCTC 1 cut(s) 921
EcoO109I RGGNCCY 1 cut(s) 723
EcoRII CCWGG 1 cut(s) 56
EcoT38I GRGCYC 1 cut(s) 923
EgeI GGCGCC 1 cut(s) 857
EheI GGCGCC 1 cut(s) 857
FaeI CATG 4 cut(s) 248, 342, 460, 676
FalI AAGNNNNNCTT 6 cut(s) 173, 205, 387, 419, 750, 782
FaqI GGGAC 4 cut(s) 240, 391, 606, 799
FatI CATG 4 cut(s) 244, 338, 456, 672
FauNDI CATATG 2 cut(s) 775, 806
FblI GTMKAC 2 cut(s) 653, 900
Fnu4HI GCNGC 1 cut(s) 447
FokI GGATG 4 cut(s) 134, 240, 503, 977
FriOI GRGCYC 1 cut(s) 923
Fsp4HI GCNGC 1 cut(s) 447
FspBI CTAG 2 cut(s) 839, 941
GlaI GCGC 1 cut(s) 857
GluI GCNGC 1 cut(s) 447
GsuI CTGGAG 1 cut(s) 60
HaeII RGCGCY 1 cut(s) 859
HaeIII GGCC 3 cut(s) 455, 639, 724
HapII CCGG 6 cut(s) 267, 597, 727, 785, 955, 997
HhaI GCGC 1 cut(s) 858
Hin1I GRCGYC 1 cut(s) 856
Hin1II CATG 4 cut(s) 248, 342, 460, 676
Hin6I GCGC 1 cut(s) 856
HinP1I GCGC 1 cut(s) 856
HincII GTYRAC 3 cut(s) 336, 654, 739
HindII GTYRAC 3 cut(s) 336, 654, 739
HindIII AAGCTT 3 cut(s) 68, 146, 686
HinfI GANTC 7 cut(s) 203, 460, 494, 566, 707, 970, 980
HpaI GTTAAC 1 cut(s) 336
HpaII CCGG 6 cut(s) 267, 597, 727, 785, 955, 997
HphI GGTGA 1 cut(s) 928
Hpy166II GTNNAC 5 cut(s) 97, 336, 654, 739, 901
Hpy188I TCNGA 6 cut(s) 12, 110, 163, 508, 571, 882
Hpy188III TCNNGA 2 cut(s) 284, 404
Hpy8I GTNNAC 5 cut(s) 97, 336, 654, 739, 901
HpyAV CCTTC 7 cut(s) 12, 218, 218, 470, 688, 692, 788
HpyCH4III ACNGT 4 cut(s) 106, 177, 616, 658
HpyCH4IV ACGT 2 cut(s) 467, 741
HpyCH4V TGCA 9 cut(s) 90, 328, 522, 580, 587, 649, 665, 810, 934
HpyF10VI GCNNNNNNNGC 2 cut(s) 51, 452
HpyF3I CTNAG 6 cut(s) 162, 285, 531, 570, 851, 922
HpySE526I ACGT 2 cut(s) 467, 741
Hsp92I GRCGYC 1 cut(s) 856
Hsp92II CATG 4 cut(s) 248, 342, 460, 676
HspAI GCGC 1 cut(s) 856
KasI GGCGCC 1 cut(s) 855
KspAI GTTAAC 1 cut(s) 336
Kzo9I GATC 3 cut(s) 604, 675, 835
LmnI GCTCC 1 cut(s) 59
Lsp1109I GCAGC 1 cut(s) 433
LweI GCATC 2 cut(s) 167, 407
MaeI CTAG 2 cut(s) 839, 941
MaeII ACGT 2 cut(s) 467, 741
MaeIII GTNAC 4 cut(s) 28, 610, 708, 950
MalI GATC 3 cut(s) 606, 677, 837
MboI GATC 3 cut(s) 604, 675, 835
MboII GAAGA 4 cut(s) 65, 810, 895, 917
MfeI CAATTG 1 cut(s) 219
MflI RGATCY 1 cut(s) 835
MhlI GDGCHC 1 cut(s) 923
MlsI TGGCCA 1 cut(s) 455
MluCI AATT 6 cut(s) 219, 357, 581, 642, 868, 874
MluNI TGGCCA 1 cut(s) 455
Mly113I GGCGCC 1 cut(s) 856
MlyI GAGTC 5 cut(s) 197, 503, 716, 964, 974
MmeI TCCRAC 1 cut(s) 926
MnlI CCTC 9 cut(s) 35, 274, 280, 320, 448, 565, 689, 781, 846
Mox20I TGGCCA 1 cut(s) 455
MroXI GAANNNNTTC 2 cut(s) 176, 762
MscI TGGCCA 1 cut(s) 455
MseI TTAA 7 cut(s) 66, 335, 356, 372, 384, 393, 825
MslI CAYNNNNRTG 1 cut(s) 914
Msp20I TGGCCA 1 cut(s) 455
MspI CCGG 6 cut(s) 267, 597, 727, 785, 955, 997
MspR9I CCNGG 5 cut(s) 58, 598, 727, 956, 998
MunI CAATTG 1 cut(s) 219
MvaI CCWGG 1 cut(s) 58
MwoI GCNNNNNNNGC 2 cut(s) 51, 452
NarI GGCGCC 1 cut(s) 856
NciI CCSGG 4 cut(s) 598, 727, 956, 998
NdeI CATATG 2 cut(s) 775, 806
NdeII GATC 3 cut(s) 604, 675, 835
NlaIII CATG 4 cut(s) 248, 342, 460, 676
NlaIV GGNNCC 4 cut(s) 55, 103, 725, 857
NmeAIII GCCGAG 2 cut(s) 194, 615
NmuCI GTSAC 2 cut(s) 610, 708
NspI RCATGY 1 cut(s) 342
PdmI GAANNNNTTC 2 cut(s) 176, 762
PfeI GAWTC 2 cut(s) 460, 566
Pfl23II CGTACG 1 cut(s) 742
PkrI GCNGC 1 cut(s) 448
PleI GAGTC 5 cut(s) 197, 502, 715, 964, 974
PluTI GGCGCC 1 cut(s) 859
PpsI GAGTC 5 cut(s) 197, 502, 715, 964, 974
Ppu21I YACGTR 1 cut(s) 468
Psp124BI GAGCTC 1 cut(s) 923
Psp6I CCWGG 1 cut(s) 56
PspGI CCWGG 1 cut(s) 56
PspLI CGTACG 1 cut(s) 742
PspN4I GGNNCC 4 cut(s) 55, 103, 725, 857
PspPI GGNCC 3 cut(s) 504, 638, 723
PstI CTGCAG 2 cut(s) 524, 651
PstNI CAGNNNCTG 1 cut(s) 569
PsuI RGATCY 1 cut(s) 835
RsaI GTAC 4 cut(s) 323, 390, 670, 744
RsaNI GTAC 4 cut(s) 322, 389, 669, 743
RseI CAYNNNNRTG 1 cut(s) 914
SacI GAGCTC 1 cut(s) 923
SalI GTCGAC 1 cut(s) 652
SaqAI TTAA 7 cut(s) 66, 335, 356, 372, 384, 393, 825
SatI GCNGC 1 cut(s) 447
Sau3AI GATC 3 cut(s) 604, 675, 835
Sau96I GGNCC 3 cut(s) 504, 638, 723
SchI GAGTC 5 cut(s) 197, 503, 716, 964, 974
ScrFI CCNGG 5 cut(s) 58, 598, 727, 956, 998
SduI GDGCHC 1 cut(s) 923
SfaNI GCATC 2 cut(s) 167, 407
SfcI CTRYAG 2 cut(s) 520, 647
SfoI GGCGCC 1 cut(s) 857
SinI GGWCC 1 cut(s) 504
SmiMI CAYNNNNRTG 1 cut(s) 914
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
Sse9I AATT 6 cut(s) 219, 357, 581, 642, 868, 874
SsiI CCGC 1 cut(s) 185
SspDI GGCGCC 1 cut(s) 855
SspI AATATT 1 cut(s) 317
SspMI CTAG 2 cut(s) 839, 941
SstI GAGCTC 1 cut(s) 923
StyD4I CCNGG 5 cut(s) 56, 596, 725, 954, 996
TaaI ACNGT 4 cut(s) 106, 177, 616, 658
TaiI ACGT 2 cut(s) 470, 744
TaqI TCGA 2 cut(s) 653, 978
TasI AATT 6 cut(s) 219, 357, 581, 642, 868, 874
TatI WGTACW 1 cut(s) 388
TfiI GAWTC 2 cut(s) 460, 566
Tru1I TTAA 7 cut(s) 66, 335, 356, 372, 384, 393, 825
Tru9I TTAA 7 cut(s) 66, 335, 356, 372, 384, 393, 825
TscAI CASTG 2 cut(s) 277, 717
TseFI GTSAC 2 cut(s) 610, 708
TseI GCWGC 1 cut(s) 446
Tsp45I GTSAC 2 cut(s) 610, 708
TspDTI ATGAA 2 cut(s) 233, 473
TspGWI ACGGA 2 cut(s) 114, 134
TspRI CASTG 2 cut(s) 277, 717
VpaK11BI GGWCC 1 cut(s) 504
XceI RCATGY 1 cut(s) 342
XmiI GTMKAC 2 cut(s) 653, 900
XmnI GAANNNNTTC 2 cut(s) 176, 762
XspI CTAG 2 cut(s) 839, 941
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.