AT3G05640

phosphatase 2C

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0001883
Physical Location & Seq
Reverse (-)
1640350 .. 1643253
2904 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G05640.3

Sequence Viewer

Length: 1077 bp
ATGGGACATTTCTCTTCCATGTTCAACGGTATAGCTAGATCCTTCTCGATCAAGAAAGCGAAGAACATCAACAGCAGCAAAAGCTACGCTAAGGAAGCCACAGATGAAATGGCGAGAGAGGCGAAGAAGAAGGAACTTATTTTGAGATCCTCTGGTTGCATTAATGCAGATGGATCTAATAACTTGGCTTCTGTTTTCTCTAGACGCGGTGAGAAAGGCGTTAATCAGGACTGTGCCATCGTCTGGGAGGGATATGGGTGTCAAGAAGACATGATATTCTGTGGGATATTCGATGGACATGGTCCCTGGGGACACTTTGTTTCTAAACAAGTCAGAAACTCAATGCCTATATCTTTGCTCTGTAACTGGAAAGAGACTCTTTCTCAGACCACAATAGCAGAACCCGATAAAGAGCTACAGCGGTTTGCAATCTGGAAATACTCATTCCTCAAAACCTGTGAAGCTGTTGATCTGGAGCTTGAGCATCACCGAAAGATAGATTCTTTCAACAGCGGTACGACCGCTCTAACCATTGTGAGACAGGGTGATGTTATTTATATAGCAAACGTCGGGGATTCACGTGCGGTATTGGCCACAGTTTCAGACGAAGGAAGCTTGGTCGCGGTTCAGCTCACCGTAGATTTCAAGCCAAACCTGCCTCAGGAGGAAGAGCGGATAATCGGATGCAACGGGAGAGTATTTTGCCTTCAAGATGAGCCAGGGGTCCACCGTGTATGGCAACCAGTAGATGAATCTCCGGGGCTCGCAATGTCAAGAGCATTCGGAGACTATTGTATCAAAGATTACGGATTGGTCTCAGTGCCTGAAGTCACTCAGAGGCATATATCCATTAGAGACCAGTTTATAATCTTGGCCACTGATGGGGTATGGGATGTGATATCAAACCAAGAGGCCATAGATATTGTTTCCTCGACGGCGGAGCGGGCAAAAGCTGCCAAGCGACTGGTACAGCAAGCAGTTAGGGCTTGGAATAGAAAGAGACGCGGAATCGCCATGGATGATATCTCTGCCGTGTGCCTCTTCTTCCATTCTTCATCGTCGTCGCCATCTCTATAG

Protein Analysis

358

Amino Acids

39.78

Weight (kDa)

6.47

Isoelectric Point (pI)

40.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PP2C PF00481 86 - 328 4.6e-41 Protein phosphatase 2C
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 866
Acc36I ACCTGC 1 cut(s) 663
AccB7I CCANNNNNTGG 1 cut(s) 243
AccBSI CCGCTC 3 cut(s) 524, 673, 943
AccII CGCG 3 cut(s) 207, 623, 1005
AclWI GGATC 3 cut(s) 33, 141, 181
AcoI YGGCCR 2 cut(s) 591, 873
AcuI CTGAAG 1 cut(s) 846
AcvI CACGTG 1 cut(s) 581
AfaI GTAC 2 cut(s) 517, 969
AfiI CCNNNNNNNGG 3 cut(s) 243, 661, 882
AgsI TTSAA 4 cut(s) 25, 508, 646, 710
AjnI CCWGG 2 cut(s) 305, 718
AluBI AGCT 8 cut(s) 35, 84, 415, 464, 478, 615, 631, 953
AluI AGCT 8 cut(s) 35, 84, 415, 464, 478, 615, 631, 953
Alw26I GTCTC 6 cut(s) 368, 532, 780, 820, 849, 994
AlwI GGATC 3 cut(s) 33, 141, 181
AlwNI CAGNNNCTG 1 cut(s) 824
AoxI GGCC 3 cut(s) 591, 873, 912
ApeKI GCWGC 2 cut(s) 75, 953
AseI ATTAAT 1 cut(s) 162
AspS9I GGNCC 2 cut(s) 302, 724
AsuC2I CCSGG 1 cut(s) 759
AsuHPI GGTGA 4 cut(s) 221, 479, 557, 625
AvaII GGWCC 2 cut(s) 302, 724
AxyI CCTNAGG 1 cut(s) 660
BaeI ACNNNNGTAYC 2 cut(s) 778, 811
BalI TGGCCA 2 cut(s) 593, 875
BanII GRGCYC 1 cut(s) 765
BarI GAAGNNNNNNTAC 2 cut(s) 690, 722
BbrPI CACGTG 1 cut(s) 581
BbsI GAAGAC 1 cut(s) 273
BbvI GCAGC 2 cut(s) 87, 940
BccI CCATC 5 cut(s) 164, 245, 287, 875, 1075
BceAI ACGGC 2 cut(s) 951, 1016
BciT130I CCWGG 2 cut(s) 307, 720
BcnI CCSGG 1 cut(s) 759
BcoDI GTCTC 6 cut(s) 368, 532, 780, 820, 849, 994
BfaI CTAG 2 cut(s) 36, 201
BfmI CTRYAG 2 cut(s) 416, 1073
BfuAI ACCTGC 1 cut(s) 663
BisI GCNGC 2 cut(s) 76, 954
BlsI GCNGC 2 cut(s) 77, 955
Bme1390I CCNGG 3 cut(s) 307, 720, 759
Bme18I GGWCC 2 cut(s) 302, 724
BmgT120I GGNCC 2 cut(s) 302, 724
BmiI GGNNCC 2 cut(s) 304, 725
BmrFI CCNGG 3 cut(s) 307, 720, 759
BmsI GCATC 2 cut(s) 493, 674
BpiI GAAGAC 1 cut(s) 273
BpmI CTGGAG 1 cut(s) 494
Bpu10I CCTNAGC 1 cut(s) 90
BpuEI CTTGAG 1 cut(s) 500
BpuMI CCSGG 1 cut(s) 759
BsaAI YACGTR 1 cut(s) 581
BsaI GGTCTC 2 cut(s) 820, 849
BsaJI CCNNGG 5 cut(s) 305, 306, 719, 758, 1014
Bsc4I CCNNNNNNNGG 3 cut(s) 243, 661, 882
Bse1I ACTGG 4 cut(s) 371, 743, 859, 969
Bse21I CCTNAGG 1 cut(s) 660
Bse3DI GCAATG 1 cut(s) 774
BseBI CCWGG 2 cut(s) 307, 720
BseDI CCNNGG 5 cut(s) 305, 306, 719, 758, 1014
BseGI GGATG 3 cut(s) 689, 898, 1024
BseLI CCNNNNNNNGG 3 cut(s) 243, 661, 882
BseMI GCAATG 1 cut(s) 774
BseMII CTCAG 4 cut(s) 398, 674, 831, 848
BseNI ACTGG 4 cut(s) 371, 743, 859, 969
BseXI GCAGC 2 cut(s) 87, 940
Bsh1236I CGCG 3 cut(s) 207, 623, 1005
Bsh1285I CGRYCG 1 cut(s) 522
BshFI GGCC 3 cut(s) 593, 875, 914
BsiEI CGRYCG 1 cut(s) 522
BsiSI CCGG 1 cut(s) 758
BslFI GGGAC 3 cut(s) 18, 288, 324
BslI CCNNNNNNNGG 3 cut(s) 243, 661, 882
BsmAI GTCTC 6 cut(s) 368, 532, 780, 820, 849, 994
BsmBI CGTCTC 1 cut(s) 994
BsmFI GGGAC 3 cut(s) 18, 288, 324
BsmI GAATGC 1 cut(s) 779
BsnI GGCC 3 cut(s) 593, 875, 914
Bso31I GGTCTC 2 cut(s) 820, 849
Bsp1286I GDGCHC 1 cut(s) 765
Bsp143I GATC 5 cut(s) 38, 48, 146, 173, 469
Bsp19I CCATGG 1 cut(s) 1014
BspANI GGCC 3 cut(s) 593, 875, 914
BspCNI CTCAG 4 cut(s) 397, 673, 830, 847
BspFNI CGCG 3 cut(s) 207, 623, 1005
BspLI GGNNCC 2 cut(s) 304, 725
BspMI ACCTGC 1 cut(s) 663
BspPI GGATC 3 cut(s) 33, 141, 181
BspQI GCTCTTC 1 cut(s) 663
BspTNI GGTCTC 2 cut(s) 820, 849
BsrBI CCGCTC 3 cut(s) 524, 673, 943
BsrDI GCAATG 1 cut(s) 774
BsrI ACTGG 4 cut(s) 371, 743, 859, 969
BssECI CCNNGG 5 cut(s) 305, 306, 719, 758, 1014
BssMI GATC 5 cut(s) 38, 48, 146, 173, 469
BssT1I CCWWGG 1 cut(s) 1014
Bst2UI CCWGG 2 cut(s) 307, 720
Bst4CI ACNGT 5 cut(s) 29, 233, 598, 637, 731
Bst6I CTCTTC 3 cut(s) 19, 663, 1046
BstAPI GCANNNNNTGC 1 cut(s) 953
BstBAI YACGTR 1 cut(s) 581
BstC8I GCNNGC 3 cut(s) 765, 945, 975
BstDEI CTNAG 5 cut(s) 90, 384, 660, 817, 834
BstDSI CCRYGG 1 cut(s) 1014
BstENI CCTNNNNNAGG 1 cut(s) 659
BstF5I GGATG 3 cut(s) 689, 898, 1024
BstFNI CGCG 3 cut(s) 207, 623, 1005
BstKTI GATC 5 cut(s) 41, 51, 149, 176, 472
BstMAI GTCTC 6 cut(s) 368, 532, 780, 820, 849, 994
BstMBI GATC 5 cut(s) 38, 48, 146, 173, 469
BstMCI CGRYCG 1 cut(s) 522
BstMWI GCNNNNNNNGC 8 cut(s) 81, 95, 119, 590, 655, 944, 953, 983
BstNI CCWGG 2 cut(s) 307, 720
BstSCI CCNGG 3 cut(s) 305, 718, 757
BstSFI CTRYAG 2 cut(s) 416, 1073
BstUI CGCG 3 cut(s) 207, 623, 1005
BstV1I GCAGC 2 cut(s) 87, 940
BstV2I GAAGAC 1 cut(s) 273
BstX2I RGATCY 3 cut(s) 38, 146, 173
BstXI CCANNNNNNTGG 1 cut(s) 964
BstYI RGATCY 3 cut(s) 38, 146, 173
Bsu36I CCTNAGG 1 cut(s) 660
BsuRI GGCC 3 cut(s) 593, 875, 914
BtgI CCRYGG 1 cut(s) 1014
BtsCI GGATG 3 cut(s) 689, 898, 1024
BtsIMutI CAGTG 2 cut(s) 825, 876
BveI ACCTGC 1 cut(s) 663
Cac8I GCNNGC 3 cut(s) 765, 945, 975
CaiI CAGNNNCTG 1 cut(s) 824
Cfr13I GGNCC 2 cut(s) 302, 724
CseI GACGC 2 cut(s) 213, 1011
Csp6I GTAC 2 cut(s) 516, 968
CviAII CATG 4 cut(s) 19, 271, 299, 1015
CviQI GTAC 2 cut(s) 516, 968
DdeI CTNAG 5 cut(s) 90, 384, 660, 817, 834
DpnI GATC 5 cut(s) 40, 50, 148, 175, 471
DpnII GATC 5 cut(s) 38, 48, 146, 173, 469
EaeI YGGCCR 2 cut(s) 591, 873
Eam1104I CTCTTC 3 cut(s) 19, 663, 1046
EarI CTCTTC 3 cut(s) 19, 663, 1046
EciI GGCGGA 1 cut(s) 953
Eco130I CCWWGG 1 cut(s) 1014
Eco24I GRGCYC 1 cut(s) 765
Eco31I GGTCTC 2 cut(s) 820, 849
Eco32I GATATC 2 cut(s) 900, 1024
Eco47I GGWCC 2 cut(s) 302, 724
Eco57I CTGAAG 1 cut(s) 846
Eco72I CACGTG 1 cut(s) 581
Eco81I CCTNAGG 1 cut(s) 660
EcoNI CCTNNNNNAGG 1 cut(s) 659
EcoRII CCWGG 2 cut(s) 305, 718
EcoRV GATATC 2 cut(s) 900, 1024
EcoT14I CCWWGG 1 cut(s) 1014
EcoT38I GRGCYC 1 cut(s) 765
ErhI CCWWGG 1 cut(s) 1014
Esp3I CGTCTC 1 cut(s) 994
FaeI CATG 4 cut(s) 22, 274, 302, 1018
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FaqI GGGAC 3 cut(s) 18, 288, 324
FatI CATG 4 cut(s) 18, 270, 298, 1014
FauI CCCGC 1 cut(s) 936
Fnu4HI GCNGC 2 cut(s) 76, 954
FokI GGATG 3 cut(s) 696, 905, 1031
FriOI GRGCYC 1 cut(s) 765
Fsp4HI GCNGC 2 cut(s) 76, 954
FspBI CTAG 2 cut(s) 36, 201
GluI GCNGC 2 cut(s) 76, 954
GsuI CTGGAG 1 cut(s) 494
HaeIII GGCC 3 cut(s) 593, 875, 914
HapII CCGG 1 cut(s) 758
HgaI GACGC 2 cut(s) 213, 1011
Hin1II CATG 4 cut(s) 22, 274, 302, 1018
HindIII AAGCTT 1 cut(s) 613
HinfI GANTC 5 cut(s) 376, 500, 575, 752, 1008
HpaII CCGG 1 cut(s) 758
HphI GGTGA 4 cut(s) 221, 479, 557, 625
Hpy166II GTNNAC 1 cut(s) 727
Hpy188I TCNGA 6 cut(s) 335, 387, 604, 683, 785, 837
Hpy8I GTNNAC 1 cut(s) 727
Hpy99I CGWCG 4 cut(s) 572, 937, 1063, 1066
HpyAV CCTTC 4 cut(s) 52, 124, 602, 716
HpyCH4III ACNGT 5 cut(s) 29, 233, 598, 637, 731
HpyCH4IV ACGT 2 cut(s) 567, 580
HpyCH4V TGCA 4 cut(s) 159, 167, 428, 687
HpyF10VI GCNNNNNNNGC 8 cut(s) 81, 95, 119, 590, 655, 944, 953, 983
HpyF3I CTNAG 5 cut(s) 90, 384, 660, 817, 834
HpySE526I ACGT 2 cut(s) 567, 580
Hsp92II CATG 4 cut(s) 22, 274, 302, 1018
Kzo9I GATC 5 cut(s) 38, 48, 146, 173, 469
LguI GCTCTTC 1 cut(s) 663
LmnI GCTCC 2 cut(s) 475, 940
Lsp1109I GCAGC 2 cut(s) 87, 940
LweI GCATC 2 cut(s) 493, 674
MaeI CTAG 2 cut(s) 36, 201
MaeII ACGT 2 cut(s) 567, 580
MaeIII GTNAC 2 cut(s) 362, 829
MalI GATC 5 cut(s) 40, 50, 148, 175, 471
MbiI CCGCTC 3 cut(s) 524, 673, 943
MboI GATC 5 cut(s) 38, 48, 146, 173, 469
MboII GAAGA 9 cut(s) 6, 73, 136, 139, 278, 680, 1033, 1036, 1044
MflI RGATCY 3 cut(s) 38, 146, 173
MhlI GDGCHC 1 cut(s) 765
MlsI TGGCCA 2 cut(s) 593, 875
MluNI TGGCCA 2 cut(s) 593, 875
MlyI GAGTC 1 cut(s) 370
Mox20I TGGCCA 2 cut(s) 593, 875
MscI TGGCCA 2 cut(s) 593, 875
MseI TTAA 2 cut(s) 162, 222
Msp20I TGGCCA 2 cut(s) 593, 875
MspA1I CMGCKG 2 cut(s) 421, 513
MspI CCGG 1 cut(s) 758
MspR9I CCNGG 3 cut(s) 307, 720, 759
Mva1269I GAATGC 1 cut(s) 779
MvaI CCWGG 2 cut(s) 307, 720
MvnI CGCG 3 cut(s) 207, 623, 1005
MwoI GCNNNNNNNGC 8 cut(s) 81, 95, 119, 590, 655, 944, 953, 983
NciI CCSGG 1 cut(s) 759
NcoI CCATGG 1 cut(s) 1014
NdeII GATC 5 cut(s) 38, 48, 146, 173, 469
NlaIII CATG 4 cut(s) 22, 274, 302, 1018
NlaIV GGNNCC 2 cut(s) 304, 725
NmuCI GTSAC 1 cut(s) 829
PasI CCCWGGG 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 663
PctI GAATGC 1 cut(s) 779
PfeI GAWTC 4 cut(s) 500, 575, 752, 1008
PflFI GACNNNGTC 1 cut(s) 300
PflMI CCANNNNNTGG 1 cut(s) 243
PkrI GCNGC 2 cut(s) 77, 955
PleI GAGTC 1 cut(s) 370
PmaCI CACGTG 1 cut(s) 581
PmlI CACGTG 1 cut(s) 581
PpsI GAGTC 1 cut(s) 370
Ppu21I YACGTR 1 cut(s) 581
PshBI ATTAAT 1 cut(s) 162
PsiI TTATAA 1 cut(s) 866
Psp6I CCWGG 2 cut(s) 305, 718
PspCI CACGTG 1 cut(s) 581
PspGI CCWGG 2 cut(s) 305, 718
PspN4I GGNNCC 2 cut(s) 304, 725
PspPI GGNCC 2 cut(s) 302, 724
PstNI CAGNNNCTG 1 cut(s) 824
PsuI RGATCY 3 cut(s) 38, 146, 173
PsyI GACNNNGTC 1 cut(s) 300
RsaI GTAC 2 cut(s) 517, 969
RsaNI GTAC 2 cut(s) 516, 968
SapI GCTCTTC 1 cut(s) 663
SaqAI TTAA 2 cut(s) 162, 222
SatI GCNGC 2 cut(s) 76, 954
Sau3AI GATC 5 cut(s) 38, 48, 146, 173, 469
Sau96I GGNCC 2 cut(s) 302, 724
SchI GAGTC 1 cut(s) 370
ScrFI CCNGG 3 cut(s) 307, 720, 759
SduI GDGCHC 1 cut(s) 765
SfaNI GCATC 2 cut(s) 493, 674
SfcI CTRYAG 2 cut(s) 416, 1073
SinI GGWCC 2 cut(s) 302, 724
SmlI CTYRAG 1 cut(s) 479
SmoI CTYRAG 1 cut(s) 479
SspMI CTAG 2 cut(s) 36, 201
StyD4I CCNGG 3 cut(s) 305, 718, 757
StyI CCWWGG 1 cut(s) 1014
TaaI ACNGT 5 cut(s) 29, 233, 598, 637, 731
TaiI ACGT 2 cut(s) 570, 583
TaqI TCGA 3 cut(s) 47, 291, 932
TfiI GAWTC 4 cut(s) 500, 575, 752, 1008
Tru1I TTAA 2 cut(s) 162, 222
Tru9I TTAA 2 cut(s) 162, 222
TscAI CASTG 2 cut(s) 825, 883
TseFI GTSAC 1 cut(s) 829
TseI GCWGC 2 cut(s) 75, 953
Tsp45I GTSAC 1 cut(s) 829
TspDTI ATGAA 3 cut(s) 120, 765, 1044
TspGWI ACGGA 1 cut(s) 822
TspRI CASTG 2 cut(s) 825, 883
Tth111I GACNNNGTC 1 cut(s) 300
Van91I CCANNNNNTGG 1 cut(s) 243
VpaK11BI GGWCC 2 cut(s) 302, 724
VspI ATTAAT 1 cut(s) 162
XagI CCTNNNNNAGG 1 cut(s) 659
XbaI TCTAGA 1 cut(s) 200
XcmI CCANNNNNNNNNTGG 1 cut(s) 106
XspI CTAG 2 cut(s) 36, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.