Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0001883

Physical Location & Seq

Genomic Coordinates Reverse (-)

28886576 .. 28889763

Gene Structure 3188 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1210000.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1119 bp

ATGGAGCAGTTATCCTCCATGTTCAGCGGGCTGGCGCGGTCGCTATCATTGAAGAAGGGGAAGAACAATGATGAGAAGTCCGGTGCAAGAGGAGCTGCAGAAGCAATGGCGAAGGAGGCGAAGAAGAATGACATGATCCTACGCTCTTCTGGGATTGTCTATGTCGATGGTTCAAATAATTTTGCTTCTGTTTTCTCGAAAAGAGGCGAGAAAGGAGTCAACCAGGATTGCTGCTTTGTGTGGGAGGGATTTGGGTTCCAAGAAGATATGATGTTTTGTGGGATTTTCGACGGGCATGGTCCATGGGGACATTTTGTGGCAAAACAGATCAGAGAAACAATGCCACCCTCTCTGTTGTGCAGTTGGCAAGAGACCCTAGCTCAAACTTCTATTGTCCCGGATCCAGATTTGGATAAAAAGTGTCACCGATTTAATGTATGGAAGGATTCCTATATCAAAACTTGCGCTGCCATTGATCACGAGCTTGGACAGCATCGTAGAATCGATTCCTTTTACAGCGGAACAACTGCCCTGAGTATTGTCAGACAGGGCGAGCTCATTTTCATAGCAAATGTTGGCGATTCTCGCGCTGTTTTGGCCACAACTTTAGATGACGGAAGCTTGGTGCCGGTTCAGCTTACTGTCGATTTCAAGCCTAGTTTGCCTCAGGAGGCGGAGAGAATAATTCAAAGCAATGGACGCGTTTTCTGTTTAGATGATGAGCCGGGAGTGCAAAGGGTGTGGCAGCCGGACGGAGAGACACCAGGACTAGCAATGTCTAGAGCCTTTGGAGATTACTGTGTGAAGGACTTTGGTCTCATTTCTGTGCCTGAAGTGTCACAGAGAAATATAACAAGCAGGGATCAGTTTGTTGTGTTGGCGACTGATGGGGTTTGGGATGTTGTTTCAAATCAAGAAGCCGTACAGATTGTAAGCTCCACTGCAGAGCGGGGGAAGGCTGCGAAGCGTCTGGTGGAGTTCGCTGCGCGTGCATGGAAGCGCAAGAGGAGGGGAATCGCGATGGACGACATTTCTGCTATTTGCCTCTTCTTTCACTCCACTTCCCCTCTGGCTGAATCCCAGCAAGTTCACCCATTTAGTATTGCCACCCCAAAATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000226 GO:0001101 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006470 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0006996 GO:0007010 GO:0007017 GO:0008150 GO:0008152 GO:0009414 GO:0009415 GO:0009628 GO:0009819 GO:0009987 GO:0010035 GO:0016020 GO:0016043 GO:0016311 GO:0019538 GO:0036211 GO:0040008 GO:0042221 GO:0043170 GO:0043393 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0045926 GO:0048519 GO:0050789 GO:0050896 GO:0051098 GO:0065007 GO:0065009 GO:0071704 GO:0071840 GO:0071944 GO:1901564 GO:1901700 GO:1904526

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

PP2C

Protein Analysis

373

Amino Acids

41.11

Weight (kDa)

6.2

Isoelectric Point (pI)

36.61

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PP2C	PF00481	136 - 330	5e-41	Protein phosphatase 2C

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001883)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G05640 AT3G05640 AT3G05640 AT5G27930 AT5G27930 AT5G27930
fragaria_vesca	FvH4_4g18870 FvH4_4g18870 FvH4_6g06970 FvH4_6g06970 FvH4_6g06970 FvH4_6g06970 FvH4_6g06970
malus_domestica	MD04G1197100.v1.1 MD12G1210000.v1.1 MD13G1162500.v1.1
prunus_persica	Prupe.1G129800_v2.0.a1 Prupe.1G129800_v2.0.a1 Prupe.6G317600_v2.0.a1
pyrus_communis	pycom04g17430 pycom12g19740 pycom13g13880
rosa_chinensis	RchiOBHm_Chr3g0456511 RchiOBHm_Chr4g0423771
rosa_laevigata	RLG00000007534 RLG00000025299
rosa_multiflora	Rmu_sc0002607.1_g000008
rosa_roxburghii	Rroxscaffold_6G00422680
rosa_rugosa	Rorug03G0013400 Rorug03G0013400 Rorug03G0013400 Rorug04G0189900 Rorug04G0190000 Rorug04G0190100 Rorug04G0190200 Rorug04G0190300
rosa_samantha	Rh3AG074000 Rh3BG075900 Rh3CG075400 Rh3DG077700 Rh4AG249900 Rh4BG255100 Rh4CG263100 Rh4DG249400
rosa_wichuraiana	Rw3G006350 Rw4G021390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	625
AccBSI	CCGCTC	1 cut(s)	949
AccII	CGCG	5 cut(s)	37, 588, 702, 988, 1019
AciI	CCGC	5 cut(s)	27, 37, 519, 674, 949
AclWI	GGATC	4 cut(s)	130, 395, 408, 870
AcoI	YGGCCR	1 cut(s)	597
AcuI	CTGAAG	1 cut(s)	852
AfaI	GTAC	1 cut(s)	924
AflIII	ACRYGT	1 cut(s)	700
AgsI	TTSAA	5 cut(s)	52, 174, 652, 689, 909
AjnI	CCWGG	2 cut(s)	222, 763
AluBI	AGCT	7 cut(s)	95, 380, 484, 556, 621, 637, 936
AluI	AGCT	7 cut(s)	95, 380, 484, 556, 621, 637, 936
Alw21I	GWGCWC	1 cut(s)	558
Alw26I	GTCTC	3 cut(s)	365, 752, 821
AlwI	GGATC	4 cut(s)	130, 395, 408, 870
AoxI	GGCC	1 cut(s)	597
ApeKI	GCWGC	6 cut(s)	95, 231, 467, 745, 959, 983
Asp700I	GAANNNNTTC	1 cut(s)	505
AspLEI	GCGC	5 cut(s)	37, 467, 590, 988, 1002
AspS9I	GGNCC	1 cut(s)	299
AsuC2I	CCSGG	2 cut(s)	398, 726
AsuHPI	GGTGA	2 cut(s)	416, 1082
AvaII	GGWCC	1 cut(s)	299
AxyI	CCTNAGG	1 cut(s)	666
BalI	TGGCCA	1 cut(s)	599
BamHI	GGATCC	1 cut(s)	400
BanI	GGYRCC	1 cut(s)	625
BanII	GRGCYC	1 cut(s)	558
BauI	CACGAG	1 cut(s)	479
Bbv12I	GWGCWC	1 cut(s)	558
BbvI	GCAGC	6 cut(s)	82, 218, 454, 757, 946, 970
BccI	CCATC	3 cut(s)	161, 881, 1015
BceAI	ACGGC	1 cut(s)	905
BcgI	CGANNNNNNTGC	2 cut(s)	1016, 1050
BciT130I	CCWGG	2 cut(s)	224, 765
BclI	TGATCA	1 cut(s)	475
BcnI	CCSGG	2 cut(s)	398, 726
BcoDI	GTCTC	3 cut(s)	365, 752, 821
BfaI	CTAG	4 cut(s)	377, 657, 770, 780
BfmI	CTRYAG	2 cut(s)	96, 942
BisI	GCNGC	6 cut(s)	96, 232, 468, 746, 960, 984
BlsI	GCNGC	6 cut(s)	97, 233, 469, 747, 961, 985
Bme1390I	CCNGG	4 cut(s)	224, 398, 726, 765
Bme18I	GGWCC	1 cut(s)	299
BmgT120I	GGNCC	1 cut(s)	299
BmiI	GGNNCC	3 cut(s)	257, 402, 627
BmrFI	CCNGG	4 cut(s)	224, 398, 726, 765
BmsI	GCATC	1 cut(s)	502
BoxI	GACNNNNGTC	1 cut(s)	813
BpuMI	CCSGG	2 cut(s)	398, 726
Bsa29I	ATCGAT	1 cut(s)	504
BsaI	GGTCTC	2 cut(s)	365, 821
BsaJI	CCNNGG	1 cut(s)	302
BsaWI	WCCGGW	1 cut(s)	80
Bse118I	RCCGGY	1 cut(s)	628
Bse21I	CCTNAGG	1 cut(s)	666
Bse3DI	GCAATG	3 cut(s)	111, 700, 780
BseBI	CCWGG	2 cut(s)	224, 765
BseCI	ATCGAT	1 cut(s)	504
BseDI	CCNNGG	1 cut(s)	302
BseGI	GGATG	1 cut(s)	904
BseMI	GCAATG	3 cut(s)	111, 700, 780
BseMII	CTCAG	2 cut(s)	524, 680
BseRI	GAGGAG	2 cut(s)	105, 1021
BseXI	GCAGC	6 cut(s)	82, 218, 454, 757, 946, 970
BseYI	CCCAGC	1 cut(s)	1080
BsgI	GTGCAG	1 cut(s)	379
Bsh1236I	CGCG	5 cut(s)	37, 588, 702, 988, 1019
Bsh1285I	CGRYCG	1 cut(s)	41
BshFI	GGCC	1 cut(s)	599
BshNI	GGYRCC	1 cut(s)	625
BshVI	ATCGAT	1 cut(s)	504
BsiEI	CGRYCG	1 cut(s)	41
BsiHKAI	GWGCWC	1 cut(s)	558
BsiSI	CCGG	5 cut(s)	81, 398, 629, 725, 749
BslFI	GGGAC	2 cut(s)	321, 380
BsmAI	GTCTC	3 cut(s)	365, 752, 821
BsmFI	GGGAC	2 cut(s)	321, 380
BsnI	GGCC	1 cut(s)	599
Bso31I	GGTCTC	2 cut(s)	365, 821
Bsp1286I	GDGCHC	1 cut(s)	558
Bsp143I	GATC	5 cut(s)	135, 327, 400, 475, 862
Bsp19I	CCATGG	1 cut(s)	302
Bsp68I	TCGCGA	1 cut(s)	1019
BspACI	CCGC	5 cut(s)	27, 37, 519, 674, 949
BspANI	GGCC	1 cut(s)	599
BspCNI	CTCAG	2 cut(s)	525, 679
BspDI	ATCGAT	1 cut(s)	504
BspFNI	CGCG	5 cut(s)	37, 588, 702, 988, 1019
BspLI	GGNNCC	3 cut(s)	257, 402, 627
BspMAI	CTGCAG	2 cut(s)	100, 946
BspPI	GGATC	4 cut(s)	130, 395, 408, 870
BspQI	GCTCTTC	1 cut(s)	151
BspT107I	GGYRCC	1 cut(s)	625
BspTNI	GGTCTC	2 cut(s)	365, 821
BsrBI	CCGCTC	1 cut(s)	949
BsrDI	GCAATG	3 cut(s)	111, 700, 780
BsrFI	RCCGGY	1 cut(s)	628
BssAI	RCCGGY	1 cut(s)	628
BssECI	CCNNGG	1 cut(s)	302
BssMI	GATC	5 cut(s)	135, 327, 400, 475, 862
BssSI	CACGAG	1 cut(s)	479
BssT1I	CCWWGG	1 cut(s)	302
Bst2BI	CACGAG	1 cut(s)	479
Bst2UI	CCWGG	2 cut(s)	224, 765
Bst4CI	ACNGT	2 cut(s)	643, 800
Bst6I	CTCTTC	2 cut(s)	151, 1052
BstC8I	GCNNGC	4 cut(s)	29, 33, 554, 990
BstDEI	CTNAG	2 cut(s)	533, 666
BstDSI	CCRYGG	1 cut(s)	302
BstF5I	GGATG	1 cut(s)	904
BstFNI	CGCG	5 cut(s)	37, 588, 702, 988, 1019
BstHHI	GCGC	5 cut(s)	37, 467, 590, 988, 1002
BstKTI	GATC	5 cut(s)	138, 330, 403, 478, 865
BstMAI	GTCTC	3 cut(s)	365, 752, 821
BstMBI	GATC	5 cut(s)	135, 327, 400, 475, 862
BstMCI	CGRYCG	1 cut(s)	41
BstNI	CCWGG	2 cut(s)	224, 765
BstPAI	GACNNNNGTC	1 cut(s)	813
BstSCI	CCNGG	4 cut(s)	222, 396, 724, 763
BstSFI	CTRYAG	2 cut(s)	96, 942
BstUI	CGCG	5 cut(s)	37, 588, 702, 988, 1019
BstV1I	GCAGC	6 cut(s)	82, 218, 454, 757, 946, 970
BstX2I	RGATCY	1 cut(s)	400
BstYI	RGATCY	1 cut(s)	400
Bsu15I	ATCGAT	1 cut(s)	504
Bsu36I	CCTNAGG	1 cut(s)	666
BsuRI	GGCC	1 cut(s)	599
BsuTUI	ATCGAT	1 cut(s)	504
BtgI	CCRYGG	1 cut(s)	302
BtgZI	GCGATG	1 cut(s)	1034
BtsCI	GGATG	1 cut(s)	904
BtsI	GCAGTG	1 cut(s)	939
BtsIMutI	CAGTG	1 cut(s)	939
BtuMI	TCGCGA	1 cut(s)	1019
Cac8I	GCNNGC	4 cut(s)	29, 33, 554, 990
CfoI	GCGC	5 cut(s)	37, 467, 590, 988, 1002
Cfr10I	RCCGGY	1 cut(s)	628
Cfr13I	GGNCC	1 cut(s)	299
ClaI	ATCGAT	1 cut(s)	504
CseI	GACGC	2 cut(s)	708, 956
Csp6I	GTAC	1 cut(s)	923
CspCI	CAANNNNNGTGG	2 cut(s)	722, 757
CviAII	CATG	5 cut(s)	19, 133, 296, 303, 993
CviQI	GTAC	1 cut(s)	923
DdeI	CTNAG	2 cut(s)	533, 666
DpnI	GATC	5 cut(s)	137, 329, 402, 477, 864
DpnII	GATC	5 cut(s)	135, 327, 400, 475, 862
EaeI	YGGCCR	1 cut(s)	597
Eam1104I	CTCTTC	2 cut(s)	151, 1052
EarI	CTCTTC	2 cut(s)	151, 1052
EciI	GGCGGA	1 cut(s)	689
Ecl136II	GAGCTC	1 cut(s)	556
Eco130I	CCWWGG	1 cut(s)	302
Eco24I	GRGCYC	1 cut(s)	558
Eco31I	GGTCTC	2 cut(s)	365, 821
Eco47I	GGWCC	1 cut(s)	299
Eco53kI	GAGCTC	1 cut(s)	556
Eco57I	CTGAAG	1 cut(s)	852
Eco81I	CCTNAGG	1 cut(s)	666
EcoICRI	GAGCTC	1 cut(s)	556
EcoRII	CCWGG	2 cut(s)	222, 763
EcoT14I	CCWWGG	1 cut(s)	302
EcoT38I	GRGCYC	1 cut(s)	558
ErhI	CCWWGG	1 cut(s)	302
FaeI	CATG	5 cut(s)	22, 136, 299, 306, 996
FaqI	GGGAC	2 cut(s)	321, 380
FatI	CATG	5 cut(s)	18, 132, 295, 302, 992
FauI	CCCGC	2 cut(s)	20, 942
FbaI	TGATCA	1 cut(s)	475
Fnu4HI	GCNGC	6 cut(s)	96, 232, 468, 746, 960, 984
FokI	GGATG	1 cut(s)	911
FriOI	GRGCYC	1 cut(s)	558
Fsp4HI	GCNGC	6 cut(s)	96, 232, 468, 746, 960, 984
FspBI	CTAG	4 cut(s)	377, 657, 770, 780
GlaI	GCGC	5 cut(s)	36, 466, 589, 987, 1001
GluI	GCNGC	6 cut(s)	96, 232, 468, 746, 960, 984
GsaI	CCCAGC	1 cut(s)	1084
HaeIII	GGCC	1 cut(s)	599
HapII	CCGG	5 cut(s)	81, 398, 629, 725, 749
HgaI	GACGC	2 cut(s)	708, 956
HhaI	GCGC	5 cut(s)	37, 467, 590, 988, 1002
Hin1II	CATG	5 cut(s)	22, 136, 299, 306, 996
Hin6I	GCGC	5 cut(s)	35, 465, 588, 986, 1000
HinP1I	GCGC	5 cut(s)	35, 465, 588, 986, 1000
HincII	GTYRAC	1 cut(s)	220
HindII	GTYRAC	1 cut(s)	220
HindIII	AAGCTT	1 cut(s)	619
HinfI	GANTC	7 cut(s)	216, 446, 501, 506, 581, 1014, 1076
HpaII	CCGG	5 cut(s)	81, 398, 629, 725, 749
HphI	GGTGA	2 cut(s)	416, 1082
Hpy166II	GTNNAC	2 cut(s)	220, 1090
Hpy188I	TCNGA	2 cut(s)	332, 545
Hpy188III	TCNNGA	7 cut(s)	196, 404, 479, 668, 780, 914, 1018
Hpy8I	GTNNAC	2 cut(s)	220, 1090
Hpy99I	CGWCG	1 cut(s)	293
HpyAV	CCTTC	5 cut(s)	49, 106, 436, 799, 949
HpyCH4III	ACNGT	2 cut(s)	643, 800
HpyCH4V	TGCA	6 cut(s)	86, 98, 360, 733, 944, 992
HpyF3I	CTNAG	2 cut(s)	533, 666
Hsp92II	CATG	5 cut(s)	22, 136, 299, 306, 996
HspAI	GCGC	5 cut(s)	35, 465, 588, 986, 1000
Ksp22I	TGATCA	1 cut(s)	475
Kzo9I	GATC	5 cut(s)	135, 327, 400, 475, 862
LguI	GCTCTTC	1 cut(s)	151
LmnI	GCTCC	3 cut(s)	4, 92, 941
Lsp1109I	GCAGC	6 cut(s)	82, 218, 454, 757, 946, 970
LweI	GCATC	1 cut(s)	502
MaeI	CTAG	4 cut(s)	377, 657, 770, 780
MaeIII	GTNAC	2 cut(s)	422, 837
MalI	GATC	5 cut(s)	137, 329, 402, 477, 864
MbiI	CCGCTC	1 cut(s)	949
MboI	GATC	5 cut(s)	135, 327, 400, 475, 862
MboII	GAAGA	7 cut(s)	64, 73, 133, 136, 138, 275, 1039
MflI	RGATCY	1 cut(s)	400
MhlI	GDGCHC	1 cut(s)	558
MlsI	TGGCCA	1 cut(s)	599
MluCI	AATT	2 cut(s)	178, 684
MluI	ACGCGT	1 cut(s)	700
MluNI	TGGCCA	1 cut(s)	599
MlyI	GAGTC	1 cut(s)	225
Mox20I	TGGCCA	1 cut(s)	599
MroXI	GAANNNNTTC	1 cut(s)	505
MscI	TGGCCA	1 cut(s)	599
MseI	TTAA	1 cut(s)	432
MslI	CAYNNNNRTG	1 cut(s)	824
Msp20I	TGGCCA	1 cut(s)	599
MspA1I	CMGCKG	2 cut(s)	27, 519
MspI	CCGG	5 cut(s)	81, 398, 629, 725, 749
MspR9I	CCNGG	4 cut(s)	224, 398, 726, 765
MvaI	CCWGG	2 cut(s)	224, 765
MvnI	CGCG	5 cut(s)	37, 588, 702, 988, 1019
NciI	CCSGG	2 cut(s)	398, 726
NcoI	CCATGG	1 cut(s)	302
NdeII	GATC	5 cut(s)	135, 327, 400, 475, 862
NlaIII	CATG	5 cut(s)	22, 136, 299, 306, 996
NlaIV	GGNNCC	3 cut(s)	257, 402, 627
NmuCI	GTSAC	2 cut(s)	422, 837
NruI	TCGCGA	1 cut(s)	1019
PciSI	GCTCTTC	1 cut(s)	151
PcsI	WCGNNNNNNNCGW	1 cut(s)	1023
PdmI	GAANNNNTTC	1 cut(s)	505
PfeI	GAWTC	6 cut(s)	446, 501, 506, 581, 1014, 1076
PfoI	TCCNGGA	1 cut(s)	396
PkrI	GCNGC	6 cut(s)	97, 233, 469, 747, 961, 985
PleI	GAGTC	1 cut(s)	224
PpsI	GAGTC	1 cut(s)	224
PshAI	GACNNNNGTC	1 cut(s)	813
Psp124BI	GAGCTC	1 cut(s)	558
Psp6I	CCWGG	2 cut(s)	222, 763
PspFI	CCCAGC	1 cut(s)	1080
PspGI	CCWGG	2 cut(s)	222, 763
PspN4I	GGNNCC	3 cut(s)	257, 402, 627
PspPI	GGNCC	1 cut(s)	299
PstI	CTGCAG	2 cut(s)	100, 946
PsuI	RGATCY	1 cut(s)	400
RruI	TCGCGA	1 cut(s)	1019
RsaI	GTAC	1 cut(s)	924
RsaNI	GTAC	1 cut(s)	923
RseI	CAYNNNNRTG	1 cut(s)	824
SacI	GAGCTC	1 cut(s)	558
SapI	GCTCTTC	1 cut(s)	151
SaqAI	TTAA	1 cut(s)	432
SatI	GCNGC	6 cut(s)	96, 232, 468, 746, 960, 984
Sau3AI	GATC	5 cut(s)	135, 327, 400, 475, 862
Sau96I	GGNCC	1 cut(s)	299
SchI	GAGTC	1 cut(s)	225
ScrFI	CCNGG	4 cut(s)	224, 398, 726, 765
SduI	GDGCHC	1 cut(s)	558
SetI	ASST	7 cut(s)	97, 382, 486, 558, 623, 639, 938
SfaNI	GCATC	1 cut(s)	502
SfcI	CTRYAG	2 cut(s)	96, 942
SinI	GGWCC	1 cut(s)	299
SmiMI	CAYNNNNRTG	1 cut(s)	824
Sse9I	AATT	2 cut(s)	178, 684
SsiI	CCGC	5 cut(s)	27, 37, 519, 674, 949
SspMI	CTAG	4 cut(s)	377, 657, 770, 780
SstI	GAGCTC	1 cut(s)	558
StyD4I	CCNGG	4 cut(s)	222, 396, 724, 763
StyI	CCWWGG	1 cut(s)	302
TaaI	ACNGT	2 cut(s)	643, 800
TaqI	TCGA	5 cut(s)	165, 197, 288, 504, 645
TasI	AATT	2 cut(s)	178, 684
TfiI	GAWTC	6 cut(s)	446, 501, 506, 581, 1014, 1076
Tru1I	TTAA	1 cut(s)	432
Tru9I	TTAA	1 cut(s)	432
TscAI	CASTG	1 cut(s)	946
TseFI	GTSAC	2 cut(s)	422, 837
TseI	GCWGC	6 cut(s)	95, 231, 467, 745, 959, 983
Tsp45I	GTSAC	2 cut(s)	422, 837
TspDTI	ATGAA	1 cut(s)	553
TspGWI	ACGGA	2 cut(s)	630, 768
TspRI	CASTG	1 cut(s)	946
VpaK11BI	GGWCC	1 cut(s)	299
XbaI	TCTAGA	1 cut(s)	779
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1066
XmnI	GAANNNNTTC	1 cut(s)	505
XspI	CTAG	4 cut(s)	377, 657, 770, 780

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.