AT3G22980

Elongation factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002828
Physical Location & Seq
Reverse (-)
8160075 .. 8163788
3714 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G22980.3

Sequence Viewer

Length: 3048 bp
ATGGATGAATCTGAAGGTAGGAAAGTTAGGAACATATGTATACTAGCACATGTAGACCACGGTAAGACGACTCTCGCCGATCACCTAATTGCTTCTTCCGGTGGCGGTGTTCTTCACCCGAGACTTGCCGGGAAGCTTAGGTTCATGGACTATCTAGATGAAGAACAGAGACGTGCAATCACGATGAAGAGCTCTTCGATTTCTCTTAAATATAAAGACTATTCTTTGAACCTGATTGATTCTCCTGGTCATATGGATTTCTGTAGTGAAGTCTCAACAGCTGCTCGGTTAAGTGACGGTGCGTTGGTTTTGGTTGATGCTGTTGAGGGAGTTCATATTCAAACGCATGCTGTGTTGCGTCAAGCTTGGATAGAGAAGTTGACTCCTTGTTTGGTGCTTAATAAGATTGATAGGTTGATTTTTGAGTTGAGGTTGAGTCCTATGGAGGCGTATACTCGTTTGATTAGGATTGTTCATGAGGTTAACGGGATTGTGAGTGCGTATAAGTCAGAGAAGTATTTGTCGGATGTTGATTCTATACTTGCTAGTCCTTCAGGGGAGTTGTCTGCTGAGAGTCTTGAGTTGTTGGAGGATGATGAAGAAGTTACTTTTCAGCCTCAGAAAGGTAATGTAGTATTCGTGTGTGCTTTAGACGGGTGGGGTTTTGGGATTGCGGAGTTTGCTAATTTTTATGCCTCCAAGCTTGGAGCTAGTGCGACTGCGTTGCAGAAATCGTTATGGGGTCCTCGTTATTATATTCCTAAGACTAAGATGATAGTAGGGAAGAAGAATTTAAGTGCTGGAAGCAAGGCGAAACCAATGTTTGTGCAGTTTGTGCTTGAGCCTTTGTGGCAGGTTTATGAAGCTGCACTAGATCCTGGTGGGGATAAAGCCGTTCTTGAGAAAGTTATCAAATCTTTCAATTTGTCTATTCCACCACGCGAACTTCAGAACAAGGATCCAAAAAACGTGCTTCAATCGGTGATGAGCCGTTGGCTTCCTCTGTCTGATGCAGTCTTGTCTATGGCTGTGAAACATCTGCCAGACCCCATTGCAGCACAGGCATATAGAATCCCACGCTTGGTTCCAGAAAGGAAAATTATTGGTGGTGATGATGTTGATTCAAGTGTCCTGGCAGAAGCAGAGCTTGTTAGAAAATCAATCGAGGCTTGTGATTCTAGCAGTGACAGTCCTTGTGTTGTGTTTGTGTCGAAAATGTTTGCTATTCCAATGAAAATGATTCCTCAAGATGGAAATCACAGAGAGAGGATGAACGGTTTAAATGATGATGACAGTAAAAGCGAGTCAGATGAGTGCTTCCTTGCATTTGCTAGGATCTTTAGCGGGGTACTTCGGGCTGGGCAAAGAGTCTTTGTAATTACCGCCCTATATGATCCCCTCAAGGGGGAATCGTCTCACAAGTACATTCAGGAAGCAGAACTGCATTCCCTATATCTTATGATGGGGCAAGGTTTAACACCAGTAACAGAGGTGAAAGCCGGAAATGTAGTGGCCATAAGAGGGCTTGGACCATATATATCAAAAAGCGCAACACTCTCATCTACTAGAAACTGTTGGCCCTTAGCCAGCATGGAGTTTCAGGTGTCTCCCACTCTTAGAGTTGCGATTGAACCATCGGATCCTGCAGATATGAGTGCTCTGATGAAAGGTCTAAGACTTCTAAACCGAGCAGATCCATTTGTGGAGATTACAGTGTCTGCTAGAGGAGAACATGTACTTGCTGCTGCTGGAGAGGTTCATCTTGAAAGATGCGTTAAGGATTTAAAAGAGAGATTTGCAAAAGTGAACCTAGAAGTCTCTCCACCTCTCGTGTCCTACAGGGAGACCATTGAAGGTGATGGATCTAATCTTTTGGAAAGTTTGAGATCTCTAAGCTTGAATTCTTCGGATTACATCGAGAAGAGAACTCCAAATGGCAGATGCATTATCAGAGTACATGTCATGAAACTACCACACGCTCTAACCAAGCTGCTTGATGAGAACACTGAATTACTTGGTGACATAATTGGAGGTAAAGGCAGTCATAGCGTTAAGATTCTAGAATCCCAAAAGCCAAGCCTCGGGGAGAATGTGGATCCCATCGAAGAATTGAAGAAACAGTTAGTTGAAGCTGGCGTTTCATCAAGTTCTGAAACCGAAAAGGATCGCGAAAAATGTAAAACCGAATGGTCGAAACTGCTCAAGAGAATTTGGGCGCTTGGTCCAAGAGAAAAAGGTCCAAACATACTATTCGCTCCAGATGGTAAAAGAATCGCCGAGGATGGATCAATGCTTGTAAGAGGCTCTCCTCATGTCTCTCAGAGACTTGGCTTCACAGAAGATTCTACTGAGACACCTGCAGAAGTATCTGAAACAGCATTGTACAGTGAAGCACTGACCCTCGAGAGCAGCATCGTCTCGGGTTTTCAGCTAGCGACAGCATCAGGACCTCTTTGTGATGAACCAATGTGGGGATTAGCTTTCACCATTGAATCTCATCTCGCTCCCGCAGAAGATGTTGAAACAGATAAACCCGAAAACTTTGGCATTTTCACAGGACAAGTCATGACAGCTGTCAAAGACGCATGTAGAGCCGCCGTGCTCCAGACAAATCCACGTATCGTCGAAGCAATGTACTTCTGCGAACTGAACACTGCACCTGAGTATCTTGGTCCAATGTACGCTGTTCTAAGCAGAAGACGAGCTAGAATACTCAAGGAAGAAATGCAAGAAGGGTCGTCTTTATTCACAGTCCACGCGTATGTTCCAGTTTCAGAAAGCTTCGGGTTTGCAGACGAGCTGAGAAAAGGAACGTCTGGTGGCGCGAGTGCTCTGATGGTTCTGAGCCATTGGGAAATGCTCGAGGAAGACCCCTTCTTTGTTCCCAAAACCGAAGAAGAGATCGAGGAGTTTGGTGACGGAGCAAGCGTGCTTCCAAACACCGCGAGGAAACTCATCAACGCCGTACGACGTAGGAAAGGATTACACGTTGAGGAGAAAGTGGTTCAATACGCCACTAAACAAAGAACCTTGGCTCGTAAAGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

1015

Amino Acids

111.95

Weight (kDa)

5.71

Isoelectric Point (pI)

44.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 8 - 223 2.5e-46 Elongation factor Tu GTP binding domain
EF-G_D2 PF22042 440 - 521 1e-07 Elongation factor G domain 2
EFG_III PF14492 537 - 606 3.6e-07 Elongation Factor G, domain III
EFL1 PF25118 657 - 751 3.9e-11 Elongation factor-like GTPase 1-like domain
EFG_C PF00679 874 - 958 6.3e-17 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 2365
Acc36I ACCTGC 2 cut(s) 844, 2365
AccI GTMKAC 3 cut(s) 40, 54, 452
AccII CGCG 5 cut(s) 942, 2169, 2759, 2825, 2945
AciI CCGC 7 cut(s) 105, 674, 1344, 1383, 2508, 2595, 2943
AcoI YGGCCR 1 cut(s) 1512
AcsI RAATTY 3 cut(s) 790, 1900, 2208
AcuI CTGAAG 3 cut(s) 33, 537, 932
AfaI GTAC 8 cut(s) 1350, 1424, 1737, 1956, 2384, 2636, 2681, 2967
AfiI CCNNNNNNNGG 9 cut(s) 623, 1081, 1250, 1403, 1404, 1405, 1521, 2081, 2471
AflIII ACRYGT 5 cut(s) 49, 1732, 1957, 2757, 2986
AjiI CACGTC 1 cut(s) 173
AjnI CCWGG 3 cut(s) 244, 877, 1131
Alw21I GWGCWC 4 cut(s) 194, 1660, 2604, 2833
AlwNI CAGNNNCTG 1 cut(s) 1718
Ama87I CYCGRG 5 cut(s) 118, 2081, 2402, 2419, 2861
AoxI GGCC 2 cut(s) 1512, 1577
ApeKI GCWGC 7 cut(s) 281, 866, 1055, 1742, 1745, 1990, 2409
ApoI RAATTY 3 cut(s) 790, 1900, 2208
ArsI GACNNNNNNTTYG 4 cut(s) 373, 405, 2861, 2893
AspLEI GCGC 3 cut(s) 1550, 2218, 2825
AspS9I GGNCC 7 cut(s) 743, 1529, 1578, 2222, 2237, 2447, 2672
AsuC2I CCSGG 1 cut(s) 130
AsuHPI GGTGA 9 cut(s) 74, 107, 994, 1121, 1504, 1868, 2030, 2476, 2927
AsuNHI GCTAGC 1 cut(s) 2431
AvaI CYCGRG 5 cut(s) 118, 2081, 2402, 2419, 2861
AvaII GGWCC 6 cut(s) 743, 1529, 2222, 2237, 2447, 2672
BaeI ACNNNNGTAYC 2 cut(s) 2648, 2681
BalI TGGCCA 1 cut(s) 1514
BamHI GGATCC 3 cut(s) 958, 1639, 2095
BanII GRGCYC 1 cut(s) 194
BauI CACGAG 1 cut(s) 1829
BbsI GAAGAC 2 cut(s) 2704, 2874
Bbv12I GWGCWC 4 cut(s) 194, 1660, 2604, 2833
BbvI GCAGC 7 cut(s) 268, 853, 1067, 1729, 1732, 1977, 2421
BccI CCATC 8 cut(s) 1244, 1456, 1642, 1853, 2108, 2255, 2276, 2830
BceAI ACGGC 4 cut(s) 878, 975, 2582, 2948
BcgI CGANNNNNNTGC 2 cut(s) 706, 740
BciT130I CCWGG 3 cut(s) 246, 879, 1133
BcnI CCSGG 1 cut(s) 130
BfmI CTRYAG 4 cut(s) 262, 1644, 1837, 2358
BfoI RGCGCY 1 cut(s) 2219
BfuAI ACCTGC 2 cut(s) 844, 2365
BglI GCCNNNNNGGC 1 cut(s) 850
BglII AGATCT 1 cut(s) 1886
BisI GCNGC 8 cut(s) 282, 867, 1056, 1743, 1746, 1991, 2410, 2595
BlsI GCNGC 8 cut(s) 283, 868, 1057, 1744, 1747, 1992, 2411, 2596
Bme1390I CCNGG 4 cut(s) 130, 246, 879, 1133
Bme18I GGWCC 6 cut(s) 743, 1529, 2222, 2237, 2447, 2672
BmeT110I CYCGRG 5 cut(s) 118, 2081, 2402, 2419, 2861
BmgBI CACGTC 1 cut(s) 173
BmgT120I GGNCC 7 cut(s) 743, 1529, 1578, 2222, 2237, 2447, 2672
BmiI GGNNCC 5 cut(s) 744, 960, 1086, 1641, 2097
BmrFI CCNGG 4 cut(s) 130, 246, 879, 1133
BmsI GCATC 6 cut(s) 307, 1000, 1760, 1931, 2421, 2450
BmtI GCTAGC 1 cut(s) 2435
BoxI GACNNNNGTC 1 cut(s) 2573
BpiI GAAGAC 2 cut(s) 2704, 2874
BpmI CTGGAG 3 cut(s) 1770, 2241, 2588
Bpu10I CCTNAGC 2 cut(s) 137, 1582
BpuEI CTTGAG 7 cut(s) 599, 860, 920, 1230, 1385, 2186, 2699
BpuMI CCSGG 1 cut(s) 130
BsaAI YACGTR 1 cut(s) 2618
BsaBI GATNNNNATC 1 cut(s) 1254
BsaI GGTCTC 1 cut(s) 1838
BsaJI CCNNGG 4 cut(s) 58, 2080, 2277, 3030
BsaWI WCCGGW 1 cut(s) 98
BsaXI ACNNNNNCTCC 4 cut(s) 1719, 1749, 2913, 2943
Bsc4I CCNNNNNNNGG 9 cut(s) 623, 1081, 1250, 1403, 1404, 1405, 1521, 2081, 2471
Bse1I ACTGG 2 cut(s) 1481, 2768
Bse3DI GCAATG 2 cut(s) 1050, 2637
Bse8I GATNNNNATC 1 cut(s) 1254
BseBI CCWGG 3 cut(s) 246, 879, 1133
BseDI CCNNGG 4 cut(s) 58, 2080, 2277, 3030
BseGI GGATG 5 cut(s) 10, 532, 598, 1275, 2287
BseJI GATNNNNATC 1 cut(s) 1254
BseLI CCNNNNNNNGG 9 cut(s) 623, 1081, 1250, 1403, 1404, 1405, 1521, 2081, 2471
BseMI GCAATG 2 cut(s) 1050, 2637
BseMII CTCAG 7 cut(s) 561, 632, 2333, 2340, 2652, 2792, 2834
BseNI ACTGG 2 cut(s) 1481, 2768
BseRI GAGGAG 4 cut(s) 1740, 2298, 2921, 3008
BseXI GCAGC 7 cut(s) 268, 853, 1067, 1729, 1732, 1977, 2421
BseYI CCCAGC 1 cut(s) 1358
BsgI GTGCAG 3 cut(s) 848, 852, 2640
Bsh1236I CGCG 5 cut(s) 942, 2169, 2759, 2825, 2945
BshFI GGCC 2 cut(s) 1514, 1579
BsiHKAI GWGCWC 4 cut(s) 194, 1660, 2604, 2833
BsiHKCI CYCGRG 5 cut(s) 118, 2081, 2402, 2419, 2861
BsiSI CCGG 3 cut(s) 99, 129, 1500
BsiWI CGTACG 1 cut(s) 2965
BslI CCNNNNNNNGG 9 cut(s) 623, 1081, 1250, 1403, 1404, 1405, 1521, 2081, 2471
BsmBI CGTCTC 3 cut(s) 163, 1419, 2422
BsmI GAATGC 1 cut(s) 1444
BsnI GGCC 2 cut(s) 1514, 1579
Bso31I GGTCTC 1 cut(s) 1838
BsoBI CYCGRG 5 cut(s) 118, 2081, 2402, 2419, 2861
Bsp1286I GDGCHC 4 cut(s) 194, 1660, 2604, 2833
Bsp1407I TGTACA 1 cut(s) 2382
Bsp68I TCGCGA 1 cut(s) 2169
BspACI CCGC 7 cut(s) 105, 674, 1344, 1383, 2508, 2595, 2943
BspANI GGCC 2 cut(s) 1514, 1579
BspCNI CTCAG 7 cut(s) 562, 631, 2332, 2341, 2653, 2793, 2835
BspFNI CGCG 5 cut(s) 942, 2169, 2759, 2825, 2945
BspHI TCATGA 3 cut(s) 475, 1962, 2565
BspLI GGNNCC 5 cut(s) 744, 960, 1086, 1641, 2097
BspMAI CTGCAG 2 cut(s) 1648, 2362
BspMI ACCTGC 2 cut(s) 844, 2365
BspOI GCTAGC 1 cut(s) 2435
BspQI GCTCTTC 2 cut(s) 182, 199
BspTNI GGTCTC 1 cut(s) 1838
BsrDI GCAATG 2 cut(s) 1050, 2637
BsrGI TGTACA 1 cut(s) 2382
BsrI ACTGG 2 cut(s) 1481, 2768
BssECI CCNNGG 4 cut(s) 58, 2080, 2277, 3030
BssNAI GTATAC 2 cut(s) 41, 453
BssSI CACGAG 1 cut(s) 1829
BssT1I CCWWGG 1 cut(s) 3030
Bst1107I GTATAC 2 cut(s) 41, 453
Bst2BI CACGAG 1 cut(s) 1829
Bst2UI CCWGG 3 cut(s) 246, 879, 1133
Bst6I CTCTTC 4 cut(s) 182, 199, 1916, 2892
BstAPI GCANNNNNTGC 1 cut(s) 835
BstAUI TGTACA 1 cut(s) 2382
BstBAI YACGTR 1 cut(s) 2618
BstC8I GCNNGC 6 cut(s) 348, 1588, 2134, 2433, 2926, 2930
BstDSI CCRYGG 1 cut(s) 58
BstENI CCTNNNNNAGG 1 cut(s) 621
BstF5I GGATG 5 cut(s) 10, 532, 598, 1275, 2287
BstFNI CGCG 5 cut(s) 942, 2169, 2759, 2825, 2945
BstH2I RGCGCY 1 cut(s) 2219
BstHHI GCGC 3 cut(s) 1550, 2218, 2825
BstMWI GCNNNNNNNGC 6 cut(s) 680, 835, 850, 1061, 2046, 2591
BstNI CCWGG 3 cut(s) 246, 879, 1133
BstNSI RCATGY 5 cut(s) 53, 350, 1736, 1961, 2589
BstPAI GACNNNNGTC 1 cut(s) 2573
BstSCI CCNGG 4 cut(s) 128, 244, 877, 1131
BstSFI CTRYAG 4 cut(s) 262, 1644, 1837, 2358
BstUI CGCG 5 cut(s) 942, 2169, 2759, 2825, 2945
BstV1I GCAGC 7 cut(s) 268, 853, 1067, 1729, 1732, 1977, 2421
BstV2I GAAGAC 2 cut(s) 2704, 2874
BstX2I RGATCY 8 cut(s) 874, 958, 1335, 1639, 1693, 1862, 1886, 2095
BstYI RGATCY 8 cut(s) 874, 958, 1335, 1639, 1693, 1862, 1886, 2095
BstZ17I GTATAC 2 cut(s) 41, 453
BsuRI GGCC 2 cut(s) 1514, 1579
BtgI CCRYGG 1 cut(s) 58
BtrI CACGTC 1 cut(s) 173
BtsCI GGATG 5 cut(s) 10, 532, 598, 1275, 2287
BtsI GCAGTG 2 cut(s) 1189, 2652
BtsIMutI CAGTG 6 cut(s) 1189, 1719, 2004, 2392, 2393, 2652
BtuMI TCGCGA 1 cut(s) 2169
BveI ACCTGC 2 cut(s) 844, 2365
Cac8I GCNNGC 6 cut(s) 348, 1588, 2134, 2433, 2926, 2930
CaiI CAGNNNCTG 1 cut(s) 1718
CciI TCATGA 3 cut(s) 475, 1962, 2565
CfoI GCGC 3 cut(s) 1550, 2218, 2825
Cfr13I GGNCC 7 cut(s) 743, 1529, 1578, 2222, 2237, 2447, 2672
CseI GACGC 2 cut(s) 347, 2591
Csp6I GTAC 8 cut(s) 1349, 1423, 1736, 1955, 2383, 2635, 2680, 2966
CviQI GTAC 8 cut(s) 1349, 1423, 1736, 1955, 2383, 2635, 2680, 2966
DraI TTTAAA 2 cut(s) 1281, 1785
EaeI YGGCCR 1 cut(s) 1512
Eam1104I CTCTTC 4 cut(s) 182, 199, 1916, 2892
EarI CTCTTC 4 cut(s) 182, 199, 1916, 2892
Ecl136II GAGCTC 1 cut(s) 192
Eco130I CCWWGG 1 cut(s) 3030
Eco24I GRGCYC 1 cut(s) 194
Eco31I GGTCTC 1 cut(s) 1838
Eco47I GGWCC 6 cut(s) 743, 1529, 2222, 2237, 2447, 2672
Eco53kI GAGCTC 1 cut(s) 192
Eco57I CTGAAG 3 cut(s) 33, 537, 932
Eco88I CYCGRG 5 cut(s) 118, 2081, 2402, 2419, 2861
EcoICRI GAGCTC 1 cut(s) 192
EcoNI CCTNNNNNAGG 1 cut(s) 621
EcoO109I RGGNCCY 2 cut(s) 743, 2447
EcoRI GAATTC 1 cut(s) 1900
EcoRII CCWGG 3 cut(s) 244, 877, 1131
EcoT14I CCWWGG 1 cut(s) 3030
EcoT22I ATGCAT 1 cut(s) 1946
EcoT38I GRGCYC 1 cut(s) 194
ErhI CCWWGG 1 cut(s) 3030
Esp3I CGTCTC 3 cut(s) 163, 1419, 2422
FalI AAGNNNNNCTT 4 cut(s) 882, 914, 1131, 1163
FauI CCCGC 2 cut(s) 1337, 2515
FauNDI CATATG 2 cut(s) 35, 252
FblI GTMKAC 3 cut(s) 40, 54, 452
Fnu4HI GCNGC 8 cut(s) 282, 867, 1056, 1743, 1746, 1991, 2410, 2595
FokI GGATG 5 cut(s) 17, 539, 605, 1282, 2294
FriOI GRGCYC 1 cut(s) 194
Fsp4HI GCNGC 8 cut(s) 282, 867, 1056, 1743, 1746, 1991, 2410, 2595
GlaI GCGC 3 cut(s) 1549, 2217, 2824
GluI GCNGC 8 cut(s) 282, 867, 1056, 1743, 1746, 1991, 2410, 2595
GsaI CCCAGC 1 cut(s) 1362
GsuI CTGGAG 3 cut(s) 1770, 2241, 2588
HaeII RGCGCY 1 cut(s) 2219
HaeIII GGCC 2 cut(s) 1514, 1579
HapII CCGG 3 cut(s) 99, 129, 1500
HgaI GACGC 2 cut(s) 347, 2591
HhaI GCGC 3 cut(s) 1550, 2218, 2825
Hin6I GCGC 3 cut(s) 1548, 2216, 2823
HinP1I GCGC 3 cut(s) 1548, 2216, 2823
HincII GTYRAC 2 cut(s) 381, 484
HindII GTYRAC 2 cut(s) 381, 484
HindIII AAGCTT 5 cut(s) 134, 363, 701, 1894, 2779
HpaI GTTAAC 1 cut(s) 484
HpaII CCGG 3 cut(s) 99, 129, 1500
HphI GGTGA 9 cut(s) 74, 107, 994, 1121, 1504, 1868, 2030, 2476, 2927
Hpy166II GTNNAC 7 cut(s) 41, 55, 381, 453, 484, 1807, 2755
Hpy8I GTNNAC 7 cut(s) 41, 55, 381, 453, 484, 1807, 2755
Hpy99I CGWCG 2 cut(s) 2627, 2973
HpyAV CCTTC 5 cut(s) 8, 561, 1847, 2726, 2884
HpyCH4IV ACGT 6 cut(s) 172, 969, 2617, 2813, 2971, 2988
HpyF10VI GCNNNNNNNGC 6 cut(s) 680, 835, 850, 1061, 2046, 2591
HpySE526I ACGT 6 cut(s) 172, 969, 2617, 2813, 2971, 2988
HspAI GCGC 3 cut(s) 1548, 2216, 2823
KspAI GTTAAC 1 cut(s) 484
LguI GCTCTTC 2 cut(s) 182, 199
LmnI GCTCC 5 cut(s) 707, 2260, 2509, 2607, 2921
Lsp1109I GCAGC 7 cut(s) 268, 853, 1067, 1729, 1732, 1977, 2421
LweI GCATC 6 cut(s) 307, 1000, 1760, 1931, 2421, 2450
MaeII ACGT 6 cut(s) 172, 969, 2617, 2813, 2971, 2988
MaeIII GTNAC 6 cut(s) 293, 604, 1184, 1483, 2018, 2915
MflI RGATCY 8 cut(s) 874, 958, 1335, 1639, 1693, 1862, 1886, 2095
MhlI GDGCHC 4 cut(s) 194, 1660, 2604, 2833
MlsI TGGCCA 1 cut(s) 1514
MluI ACGCGT 1 cut(s) 2757
MluNI TGGCCA 1 cut(s) 1514
MlyI GAGTC 6 cut(s) 64, 376, 445, 583, 1313, 1377
MmeI TCCRAC 2 cut(s) 504, 567
Mox20I TGGCCA 1 cut(s) 1514
Mph1103I ATGCAT 1 cut(s) 1946
MscI TGGCCA 1 cut(s) 1514
MslI CAYNNNNRTG 1 cut(s) 2760
Msp20I TGGCCA 1 cut(s) 1514
MspA1I CMGCKG 2 cut(s) 281, 2573
MspI CCGG 3 cut(s) 99, 129, 1500
MspR9I CCNGG 4 cut(s) 130, 246, 879, 1133
Mva1269I GAATGC 1 cut(s) 1444
MvaI CCWGG 3 cut(s) 246, 879, 1133
MvnI CGCG 5 cut(s) 942, 2169, 2759, 2825, 2945
MwoI GCNNNNNNNGC 6 cut(s) 680, 835, 850, 1061, 2046, 2591
NciI CCSGG 1 cut(s) 130
NdeI CATATG 2 cut(s) 35, 252
NheI GCTAGC 1 cut(s) 2431
NlaIV GGNNCC 5 cut(s) 744, 960, 1086, 1641, 2097
NmeAIII GCCGAG 1 cut(s) 2302
NmuCI GTSAC 4 cut(s) 293, 1184, 2018, 2915
NruI TCGCGA 1 cut(s) 2169
NsiI ATGCAT 1 cut(s) 1946
NspI RCATGY 5 cut(s) 53, 350, 1736, 1961, 2589
PaeI GCATGC 1 cut(s) 350
PaeR7I CTCGAG 2 cut(s) 2402, 2861
PagI TCATGA 3 cut(s) 475, 1962, 2565
PaqCI CACCTGC 1 cut(s) 2365
PciI ACATGT 3 cut(s) 49, 1732, 1957
PciSI GCTCTTC 2 cut(s) 182, 199
PcsI WCGNNNNNNNCGW 1 cut(s) 2925
PctI GAATGC 1 cut(s) 1444
Pfl23II CGTACG 1 cut(s) 2965
PkrI GCNGC 8 cut(s) 283, 868, 1057, 1744, 1747, 1992, 2411, 2596
PleI GAGTC 6 cut(s) 64, 376, 444, 582, 1312, 1376
PpsI GAGTC 6 cut(s) 64, 376, 444, 582, 1312, 1376
Ppu21I YACGTR 1 cut(s) 2618
PpuMI RGGWCCY 2 cut(s) 743, 2447
PscI ACATGT 3 cut(s) 49, 1732, 1957
PshAI GACNNNNGTC 1 cut(s) 2573
Psp124BI GAGCTC 1 cut(s) 194
Psp5II RGGWCCY 2 cut(s) 743, 2447
Psp6I CCWGG 3 cut(s) 244, 877, 1131
PspFI CCCAGC 1 cut(s) 1358
PspGI CCWGG 3 cut(s) 244, 877, 1131
PspLI CGTACG 1 cut(s) 2965
PspN4I GGNNCC 5 cut(s) 744, 960, 1086, 1641, 2097
PspPI GGNCC 7 cut(s) 743, 1529, 1578, 2222, 2237, 2447, 2672
PspPPI RGGWCCY 2 cut(s) 743, 2447
PspXI VCTCGAGB 1 cut(s) 2861
PstI CTGCAG 2 cut(s) 1648, 2362
PstNI CAGNNNCTG 1 cut(s) 1718
PsuI RGATCY 8 cut(s) 874, 958, 1335, 1639, 1693, 1862, 1886, 2095
PvuII CAGCTG 2 cut(s) 281, 2573
RruI TCGCGA 1 cut(s) 2169
RsaI GTAC 8 cut(s) 1350, 1424, 1737, 1956, 2384, 2636, 2681, 2967
RsaNI GTAC 8 cut(s) 1349, 1423, 1736, 1955, 2383, 2635, 2680, 2966
RseI CAYNNNNRTG 1 cut(s) 2760
SacI GAGCTC 1 cut(s) 194
SapI GCTCTTC 2 cut(s) 182, 199
SatI GCNGC 8 cut(s) 282, 867, 1056, 1743, 1746, 1991, 2410, 2595
Sau96I GGNCC 7 cut(s) 743, 1529, 1578, 2222, 2237, 2447, 2672
SchI GAGTC 6 cut(s) 64, 376, 445, 583, 1313, 1377
ScrFI CCNGG 4 cut(s) 130, 246, 879, 1133
SduI GDGCHC 4 cut(s) 194, 1660, 2604, 2833
SfaNI GCATC 6 cut(s) 307, 1000, 1760, 1931, 2421, 2450
SfcI CTRYAG 4 cut(s) 262, 1644, 1837, 2358
Sfr274I CTCGAG 2 cut(s) 2402, 2861
SinI GGWCC 6 cut(s) 743, 1529, 2222, 2237, 2447, 2672
SlaI CTCGAG 2 cut(s) 2402, 2861
SmiMI CAYNNNNRTG 1 cut(s) 2760
SmlI CTYRAG 9 cut(s) 578, 839, 899, 1245, 1400, 2201, 2402, 2714, 2861
SmoI CTYRAG 9 cut(s) 578, 839, 899, 1245, 1400, 2201, 2402, 2714, 2861
SphI GCATGC 1 cut(s) 350
SsiI CCGC 7 cut(s) 105, 674, 1344, 1383, 2508, 2595, 2943
SstI GAGCTC 1 cut(s) 194
StyD4I CCNGG 4 cut(s) 128, 244, 877, 1131
StyI CCWWGG 1 cut(s) 3030
TaiI ACGT 6 cut(s) 175, 972, 2620, 2816, 2974, 2991
TatI WGTACW 5 cut(s) 1422, 1735, 1954, 2382, 2634
TauI GCSGC 1 cut(s) 2597
TscAI CASTG 6 cut(s) 1189, 1719, 2011, 2392, 2400, 2659
TseFI GTSAC 4 cut(s) 293, 1184, 2018, 2915
TseI GCWGC 7 cut(s) 281, 866, 1055, 1742, 1745, 1990, 2409
Tsp45I GTSAC 4 cut(s) 293, 1184, 2018, 2915
TspGWI ACGGA 1 cut(s) 2934
TspRI CASTG 6 cut(s) 1189, 1719, 2011, 2392, 2400, 2659
VpaK11BI GGWCC 6 cut(s) 743, 1529, 2222, 2237, 2447, 2672
XagI CCTNNNNNAGG 1 cut(s) 621
XapI RAATTY 3 cut(s) 790, 1900, 2208
XbaI TCTAGA 2 cut(s) 154, 2059
XceI RCATGY 5 cut(s) 53, 350, 1736, 1961, 2589
XhoI CTCGAG 2 cut(s) 2402, 2861
XmiI GTMKAC 3 cut(s) 40, 54, 452
Zsp2I ATGCAT 1 cut(s) 1946
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.