pycom05g23810

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002828
Physical Location & Seq
Forward (+)
25554486 .. 25554902
417 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g23810.1

Sequence Viewer

Length: 417 bp
ATGTACTTCTGCGAATTGAACACGACAACTGCACATTTGGGTTCTATGTACGCTGTGCTTGGGCGAAGGCGGGCTAGAGTTTTAAAGGAGGAAATGCGGGAAGGCTCCCCATTGTTCACTGTGCATGCATATGTTCCTGTTTCTGAAAGCTTTGGTTTTGTGGATGAGCTTAGAAGATGGACTGCTGGTGCTGCAAGCGCTCTTCTTGTGCTTAGTCAATGGGCAGCACTTCCAGAGGATCCTTTCTTTGTACCGAAGACAGAAGAAGAACTTGAGGAGCTTGGTGACGGTTCTAGTGTGCTTCCCAATACTGCAAGGAAACTAATTAACGCTGTAAGACGGAAGAAGGGGCTTCCTGTAGAGGAAAAATTAGTACAGCACGCGACCAAGCAGAGGACCCTGGCTCGTAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

139

Amino Acids

15.51

Weight (kDa)

9.16

Isoelectric Point (pI)

65.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFG_C PF00679 1 - 81 4.1e-14 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 383
AciI CCGC 2 cut(s) 70, 97
AclWI GGATC 2 cut(s) 233, 246
AfaI GTAC 4 cut(s) 5, 50, 252, 375
AfeI AGCGCT 1 cut(s) 199
AfiI CCNNNNNNNGG 1 cut(s) 393
AgsI TTSAA 1 cut(s) 19
AjnI CCWGG 1 cut(s) 399
AluBI AGCT 3 cut(s) 150, 169, 280
AluI AGCT 3 cut(s) 150, 169, 280
AlwI GGATC 2 cut(s) 233, 246
Aor51HI AGCGCT 1 cut(s) 199
ApeKI GCWGC 2 cut(s) 191, 224
AspLEI GCGC 1 cut(s) 200
AspS9I GGNCC 1 cut(s) 396
AsuHPI GGTGA 1 cut(s) 296
AvaII GGWCC 1 cut(s) 396
BamHI GGATCC 1 cut(s) 238
BbsI GAAGAC 1 cut(s) 263
BbvI GCAGC 2 cut(s) 178, 236
BccI CCATC 1 cut(s) 171
BciT130I CCWGG 1 cut(s) 401
BfaI CTAG 2 cut(s) 75, 294
BfmI CTRYAG 1 cut(s) 357
BfoI RGCGCY 1 cut(s) 201
BisI GCNGC 2 cut(s) 192, 225
BlsI GCNGC 2 cut(s) 193, 226
Bme1390I CCNGG 1 cut(s) 401
Bme18I GGWCC 1 cut(s) 396
BmgT120I GGNCC 1 cut(s) 396
BmiI GGNNCC 3 cut(s) 106, 240, 398
BmrFI CCNGG 1 cut(s) 401
BpiI GAAGAC 1 cut(s) 263
BpuEI CTTGAG 1 cut(s) 293
BsaJI CCNNGG 1 cut(s) 399
Bsc4I CCNNNNNNNGG 1 cut(s) 393
BseBI CCWGG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 399
BseGI GGATG 1 cut(s) 169
BseLI CCNNNNNNNGG 1 cut(s) 393
BseRI GAGGAG 1 cut(s) 290
BseXI GCAGC 2 cut(s) 178, 236
BsgI GTGCAG 1 cut(s) 15
Bsh1236I CGCG 1 cut(s) 383
BslI CCNNNNNNNGG 1 cut(s) 393
Bsp143I GATC 1 cut(s) 238
BspACI CCGC 2 cut(s) 70, 97
BspFNI CGCG 1 cut(s) 383
BspLI GGNNCC 3 cut(s) 106, 240, 398
BspPI GGATC 2 cut(s) 233, 246
BspQI GCTCTTC 1 cut(s) 207
BssECI CCNNGG 1 cut(s) 399
BssMI GATC 1 cut(s) 238
Bst2UI CCWGG 1 cut(s) 401
Bst4CI ACNGT 2 cut(s) 121, 290
Bst6I CTCTTC 1 cut(s) 207
BstC8I GCNNGC 4 cut(s) 72, 126, 196, 381
BstDEI CTNAG 2 cut(s) 170, 212
BstF5I GGATG 1 cut(s) 169
BstFNI CGCG 1 cut(s) 383
BstH2I RGCGCY 1 cut(s) 201
BstHHI GCGC 1 cut(s) 200
BstKTI GATC 1 cut(s) 241
BstMBI GATC 1 cut(s) 238
BstMWI GCNNNNNNNGC 2 cut(s) 191, 197
BstNI CCWGG 1 cut(s) 401
BstNSI RCATGY 1 cut(s) 128
BstSCI CCNGG 1 cut(s) 399
BstSFI CTRYAG 1 cut(s) 357
BstUI CGCG 1 cut(s) 383
BstV1I GCAGC 2 cut(s) 178, 236
BstV2I GAAGAC 1 cut(s) 263
BstX2I RGATCY 1 cut(s) 238
BstYI RGATCY 1 cut(s) 238
BtsCI GGATG 1 cut(s) 169
BtsIMutI CAGTG 1 cut(s) 117
Cac8I GCNNGC 4 cut(s) 72, 126, 196, 381
CfoI GCGC 1 cut(s) 200
Cfr13I GGNCC 1 cut(s) 396
Csp6I GTAC 4 cut(s) 4, 49, 251, 374
CviAII CATG 1 cut(s) 125
CviJI RGCY 7 cut(s) 74, 105, 150, 169, 280, 352, 404
CviKI_1 RGCY 7 cut(s) 74, 105, 150, 169, 280, 352, 404
CviQI GTAC 4 cut(s) 4, 49, 251, 374
DdeI CTNAG 2 cut(s) 170, 212
DpnI GATC 1 cut(s) 240
DpnII GATC 1 cut(s) 238
DraI TTTAAA 1 cut(s) 84
Eam1104I CTCTTC 1 cut(s) 207
EarI CTCTTC 1 cut(s) 207
Eco47I GGWCC 1 cut(s) 396
Eco47III AGCGCT 1 cut(s) 199
EcoO109I RGGNCCY 1 cut(s) 396
EcoRII CCWGG 1 cut(s) 399
EcoT22I ATGCAT 1 cut(s) 130
FaeI CATG 1 cut(s) 128
FaiI YATR 4 cut(s) 47, 126, 130, 132
FalI AAGNNNNNCTT 2 cut(s) 255, 287
FatI CATG 1 cut(s) 124
FauI CCCGC 2 cut(s) 63, 90
FauNDI CATATG 1 cut(s) 130
Fnu4HI GCNGC 2 cut(s) 192, 225
FokI GGATG 1 cut(s) 176
Fsp4HI GCNGC 2 cut(s) 192, 225
FspBI CTAG 2 cut(s) 75, 294
GlaI GCGC 1 cut(s) 199
GluI GCNGC 2 cut(s) 192, 225
HaeII RGCGCY 1 cut(s) 201
HhaI GCGC 1 cut(s) 200
Hin1II CATG 1 cut(s) 128
Hin6I GCGC 1 cut(s) 198
HinP1I GCGC 1 cut(s) 198
HindIII AAGCTT 1 cut(s) 148
HphI GGTGA 1 cut(s) 296
Hpy166II GTNNAC 1 cut(s) 117
Hpy188I TCNGA 1 cut(s) 145
Hpy188III TCNNGA 1 cut(s) 233
Hpy8I GTNNAC 1 cut(s) 117
HpyAV CCTTC 3 cut(s) 60, 95, 340
HpyCH4III ACNGT 2 cut(s) 121, 290
HpyCH4V TGCA 5 cut(s) 32, 124, 128, 194, 314
HpyF10VI GCNNNNNNNGC 2 cut(s) 191, 197
HpyF3I CTNAG 2 cut(s) 170, 212
Hsp92II CATG 1 cut(s) 128
HspAI GCGC 1 cut(s) 198
Kzo9I GATC 1 cut(s) 238
LguI GCTCTTC 1 cut(s) 207
LmnI GCTCC 2 cut(s) 110, 277
LpnPI CCDG 6 cut(s) 150, 171, 246, 369, 386, 413
Lsp1109I GCAGC 2 cut(s) 178, 236
MaeI CTAG 2 cut(s) 75, 294
MaeIII GTNAC 1 cut(s) 284
MalI GATC 1 cut(s) 240
MboI GATC 1 cut(s) 238
MboII GAAGA 6 cut(s) 186, 194, 268, 275, 278, 355
MflI RGATCY 1 cut(s) 238
MluCI AATT 3 cut(s) 14, 324, 368
MnlI CCTC 5 cut(s) 82, 229, 268, 355, 387
Mph1103I ATGCAT 1 cut(s) 130
MseI TTAA 2 cut(s) 83, 327
MslI CAYNNNNRTG 1 cut(s) 129
MspR9I CCNGG 1 cut(s) 401
MvaI CCWGG 1 cut(s) 401
MvnI CGCG 1 cut(s) 383
MwoI GCNNNNNNNGC 2 cut(s) 191, 197
NdeI CATATG 1 cut(s) 130
NdeII GATC 1 cut(s) 238
NlaIII CATG 1 cut(s) 128
NlaIV GGNNCC 3 cut(s) 106, 240, 398
NmuCI GTSAC 1 cut(s) 284
NsiI ATGCAT 1 cut(s) 130
NspI RCATGY 1 cut(s) 128
PaeI GCATGC 1 cut(s) 128
PciSI GCTCTTC 1 cut(s) 207
PkrI GCNGC 2 cut(s) 193, 226
PpuMI RGGWCCY 1 cut(s) 396
Psp5II RGGWCCY 1 cut(s) 396
Psp6I CCWGG 1 cut(s) 399
PspGI CCWGG 1 cut(s) 399
PspN4I GGNNCC 3 cut(s) 106, 240, 398
PspPI GGNCC 1 cut(s) 396
PspPPI RGGWCCY 1 cut(s) 396
PsuI RGATCY 1 cut(s) 238
RsaI GTAC 4 cut(s) 5, 50, 252, 375
RsaNI GTAC 4 cut(s) 4, 49, 251, 374
RseI CAYNNNNRTG 1 cut(s) 129
SapI GCTCTTC 1 cut(s) 207
SaqAI TTAA 2 cut(s) 83, 327
SatI GCNGC 2 cut(s) 192, 225
Sau3AI GATC 1 cut(s) 238
Sau96I GGNCC 1 cut(s) 396
ScrFI CCNGG 1 cut(s) 401
SetI ASST 3 cut(s) 152, 171, 282
SfcI CTRYAG 1 cut(s) 357
SinI GGWCC 1 cut(s) 396
SmiMI CAYNNNNRTG 1 cut(s) 129
SmlI CTYRAG 1 cut(s) 272
SmoI CTYRAG 1 cut(s) 272
SphI GCATGC 1 cut(s) 128
Sse9I AATT 3 cut(s) 14, 324, 368
SsiI CCGC 2 cut(s) 70, 97
SspMI CTAG 2 cut(s) 75, 294
StyD4I CCNGG 1 cut(s) 399
TaaI ACNGT 2 cut(s) 121, 290
TasI AATT 3 cut(s) 14, 324, 368
TatI WGTACW 2 cut(s) 3, 373
Tru1I TTAA 2 cut(s) 83, 327
Tru9I TTAA 2 cut(s) 83, 327
TscAI CASTG 1 cut(s) 124
TseFI GTSAC 1 cut(s) 284
TseI GCWGC 2 cut(s) 191, 224
Tsp45I GTSAC 1 cut(s) 284
TspGWI ACGGA 1 cut(s) 355
TspRI CASTG 1 cut(s) 124
VpaK11BI GGWCC 1 cut(s) 396
XceI RCATGY 1 cut(s) 128
XspI CTAG 2 cut(s) 75, 294
Zsp2I ATGCAT 1 cut(s) 130
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.