Rroxscaffold_7G00193950

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000007
OG0002828
Physical Location & Seq
Forward (+)
35595416 .. 35599740
4325 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_7G00193950.1

Sequence Viewer

Length: 2982 bp
ATGGCCGACCCGTCCCAACAACCCCAACCTGAATCAACCACACCCGACACCCGAAAGCTCCGAAACATGTGCGTCCTGGCCCACGTCGACCACGGCAAGACGACCCTCTGCGACCACCTCATCTCCGGCTCCGACAGCGGCATCATCCACCCCAAGCTCGCCGGCCGCCTCCGCTACATGGACTCCCTCCTCGAGGAGCAGCGCCGCGCCATCACCATGAAGAGCTCCTCCATCGCCCTCCGCCACTGCGACCACTCCGTCACCGTCATCGACTCCCCGGGCCACATGGACTTCTGCAGCGAAGTCTCCACCGCCGCCAGGCTCAGCGACGGCGCGTTGATCCTAGTCGACGCCGTCGAGGGCGTCCACATCCAAACGCACGCCGTCTTGCGCCAGGCCTGGATCGAGAAGCTCACGCCGTGCTTGGTGATCAACAAGATCGATAGGTTGATCGCCGAGCTGGGAATGAGCCCTATGGATGCGTATGTAAAGTTGTTGAAGATTGTTCATGAGCCCCAGAAGGGCAATGTGGCCTTTGTGTGTGCTTTGGATGGTTGGGGGTTTAGTATTAGGCAATTTGCCGAGTTTTATGAGGCGAAATTCGGGTGGAGTACTAATGCATTGCAGAAGGCTTTATGGGGGCCTTGGTATTTTAATCACAAGGAGAAGAAGATTGTGGGACAGAAGGGTGTGGCGGGTTTGAAAAAGGCTCGGCCGATGTTTGTTGAGTTTGTGCTTGAGCCGGTTTGGAGTGTGTACCAGGCGGCTTTGAAGGAGAAGGAGGAGGCTCAAGTGATGGTTCAGAAGGTGATTAGAACGTTTAATTTGACTATACCACCTCGGGATTTGAAGGGTGACCCAAAAGGTATGGTTCAGGCTGTGATGAGTCGGTGGCTTCCTCTGCATGAGGCTATCTTGAACATGGTTATTAGGTGTATGCCTGACCCGATTGCAGCACAGTCATTTCGGATTACAAGGTTACTTCCAAAGAGACAGGTTGTGGGAGATGGGGTTGATGCTAATGTACTTGCAGAGGCGGAGCTTGTGAGAAGTTCGGTGGAAGCTTGTGATTCTAGGCCTGAAGCTCCTTGTGTTGTTTTTGTTTCGAAAATGTTTGCTGTGCCAACGAAAACACTTCCAAAGTATGGCTTGGATGGGGAGATTTTGGATAATACATCTGATGAAGGTGAATTGGATGAGTGCTTCCTTGCGTTTGCAAGGATCTATAGTGGGGTTCTTCGTCCTGGTGAGAAAATTTATGTGCTTTCAGCTTTATATGATCCCTTAAAAGGGGAATCAATGCAGAAGCATATTCAGGTGGCTAAGTTGCATTCTCTGTATCTCATGATGGGCCAAAATTTACAGCATGTGCCAGAGGCGCAGGCAGGGGATATTGTGGCAATCCGAGGCCTTGGGCAGCATATACTGAAAACTGCAACACTTTCATCAACAAAGAACTGTTGGCCTTTCTCCAGTATGTCATTCCAGGTTTCTCCCACTCTGAAAGTTGCAATTGAGCCATCTGATCCTGCAGATATGGGTGCATTGATGAAGGGTTTACGGCTGCTGAACAGGGCAGACCCTTTTGTAGAGGTCACGGTTTCTGCTAGAGGAGAACATGTTCTGTCTGCTGCAGGAGAAGTACATCTTGAAAGATGCATAAAAGATTTGAAGGATAGATTTGCAAGGGTAGGCTTGGAAGTGTCTAAGCCTCTTGTGTCTTTTAAAGAGACCATTTTGGGAGATGAATCCACACTTGAGAACTTAAAATCCTTTGTTGCCAGTTCAGAATATGTTGAGAAAGTGACACAAAATGGAAGGTGTGTTGTTCGGGTGAAGGTCCTGAAGCTCCCACCTTCTCTAACCAAGGTGATTGATGAAAGTTCTGATATTCTAGCAGATATCCTTGGAGGCAAGGCTGCCCAGACAGGAGAGACCAATAAAAGTTTAAACACACAGGTTGCATGCACTGTAGAGGATGCGAATCCAATTGAAACACTAAGGAAACGTATGATGGAAGCTGTGGAAAGTGATATTTTGTCTAGTGGGGAGACTGACAAAGATCGGGCTGAGAAATGTAAAGTCCAATGGCAAAAACTTCTTAAAAGGATCTGGGCACTTGGCCCTTGGCATATTGGCCCTAACATCCTACTTGCTCCAGAAATGAAAGGAAAGGGTACTGACTCCTCTGTGCTCATCCGCGGCTCCTCTCATGTTTCTGAGAAATTAGGATTGGTGGATGCTTCTGACAATGATAATGCAGCTGCTGACACATCTTCAGAAGTAACTGAAGCATTGTATGCTGAAGCAGAACGCCTTCATAGCAGCGTTTTATCTGGGTTTCAACTGGCAACAGCAGCTGGACCTTTATGTGACGAGCCTATGTGGGGTTTAGCATTTGTGGTTGAAGCTTACATTTCACCATTAGCTGCTCAGTCTGATGAGTTAGAAACCTCCCACCAGCAACCTGAGCAATATGGTATCTTCACAGGACAGGTAATGACAGCCATTAAGGATGCTTGTAGAGTAGCTGTGCTTCAAAAGAAACCACGGCTTGTGGAAGCTATGTACTTCTGTGAGTTGATCACGCCAACTGAACAATTGGGTAATATGTATGCTGTGCTTGGACGAAGGCGGACGAAAGTTTTAAAGGAGGAAATGCAGGAAGGCTCTGCATTGTTCACTGTACATGCCTATGTGCCGGTTTCTGAAAGCTTTGGTTTTGCGGATGAGCTTAGAAGGTGGACTGCTGGAGCTGCAAGTGCTCTTCTTGTGCTTAGCCACTGGGAAGCTCTTGATAAGGATCCTTTCTTTGTACCAAAAACGGATGAGGAAAAAGAGGAGTTTGGTGATGGTTCTAGTGTGCCTCCAAATACAGCAAGAAAACTGATAAATGCTGTAAGACGGCAGAAGGGTCTTCCAGTGGAGGAAAAAGTAGTGCAGCATGCGACAAAGCAGAGGACCTTGGCTCGTAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

993

Amino Acids

109.6

Weight (kDa)

6.38

Isoelectric Point (pI)

42.15

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 19 - 189 2e-44 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 403 - 481 1.7e-10 Elongation factor Tu domain 2
EF-G_D2 PF22042 403 - 481 5.5e-08 Elongation factor G domain 2
EFG_III PF14492 499 - 561 1.4e-06 Elongation Factor G, domain III
EFL1 PF25118 617 - 718 1e-08 Elongation factor-like GTPase 1-like domain
EFG_C PF00679 851 - 936 4.7e-16 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AbsI CCTCGAGG 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 1145
AccI GTMKAC 2 cut(s) 87, 348
AccII CGCG 3 cut(s) 207, 335, 2202
AclI AACGTT 1 cut(s) 818
AclWI GGATC 8 cut(s) 334, 410, 1229, 1274, 1520, 2117, 2798, 2811
AcoI YGGCCR 3 cut(s) 3, 163, 713
AcsI RAATTY 3 cut(s) 599, 1254, 1357
AcuI CTGAAG 5 cut(s) 1101, 1865, 2262, 2310, 2325
AcyI GRCGYC 2 cut(s) 351, 363
AdeI CACNNNGTG 1 cut(s) 420
AfaI GTAC 8 cut(s) 613, 758, 1026, 1644, 2179, 2570, 2688, 2817
AfiI CCNNNNNNNGG 8 cut(s) 178, 193, 318, 521, 1145, 1289, 1290, 2387
AflIII ACRYGT 2 cut(s) 66, 1618
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 7 cut(s) 75, 317, 393, 398, 759, 1243, 1485
AjuI GAANNNNNNNTTGG 2 cut(s) 2216, 2248
AloI GAACNNNNNNTCC 4 cut(s) 1605, 1637, 1754, 1786
Alw21I GWGCWC 3 cut(s) 227, 2196, 2767
Alw26I GTCTC 5 cut(s) 310, 985, 1724, 1928, 2045
AlwI GGATC 8 cut(s) 334, 410, 1229, 1274, 1520, 2117, 2798, 2811
AlwNI CAGNNNCTG 2 cut(s) 2267, 2360
Ama87I CYCGRG 3 cut(s) 191, 277, 840
ApoI RAATTY 3 cut(s) 599, 1254, 1357
Asp700I GAANNNNTTC 2 cut(s) 1620, 2316
AspLEI GCGC 5 cut(s) 204, 209, 335, 393, 1381
AspS9I GGNCC 9 cut(s) 79, 280, 641, 1351, 1840, 2123, 2138, 2363, 2961
AsuC2I CCSGG 2 cut(s) 278, 279
AsuII TTCGAA 1 cut(s) 1106
AvaI CYCGRG 3 cut(s) 191, 277, 840
AvaII GGWCC 3 cut(s) 1840, 2363, 2961
BaeGI GKGCMC 1 cut(s) 2119
BamHI GGATCC 1 cut(s) 2803
BanII GRGCYC 3 cut(s) 227, 473, 516
BarI GAAGNNNNNNTAC 2 cut(s) 2520, 2552
BbsI GAAGAC 1 cut(s) 2909
Bbv12I GWGCWC 3 cut(s) 227, 2196, 2767
BceAI ACGGC 8 cut(s) 109, 338, 346, 368, 403, 1577, 2567, 2921
BcgI CGANNNNNNTGC 2 cut(s) 51, 85
BciT130I CCWGG 7 cut(s) 77, 319, 395, 400, 761, 1245, 1487
BclI TGATCA 2 cut(s) 429, 2583
BcnI CCSGG 2 cut(s) 278, 279
BcoDI GTCTC 5 cut(s) 310, 985, 1724, 1928, 2045
BfaI CTAG 6 cut(s) 344, 1074, 1608, 1895, 2043, 2859
BfmI CTRYAG 5 cut(s) 295, 1225, 1530, 1632, 1971
BfoI RGCGCY 1 cut(s) 205
BlpI GCTNAGC 2 cut(s) 323, 2777
BmcAI AGTACT 1 cut(s) 613
Bme1390I CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 761, 1245, 1487
Bme18I GGWCC 3 cut(s) 1840, 2363, 2961
BmeT110I CYCGRG 3 cut(s) 191, 277, 840
BmgBI CACGTC 1 cut(s) 85
BmgT120I GGNCC 9 cut(s) 79, 280, 641, 1351, 1840, 2123, 2138, 2363, 2961
BmiI GGNNCC 4 cut(s) 130, 642, 2206, 2805
BmrFI CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 761, 1245, 1487
BmrI ACTGGG 1 cut(s) 2794
BmsI GCATC 7 cut(s) 150, 469, 1006, 1646, 1969, 2230, 2506
BmuI ACTGGG 1 cut(s) 2794
BpiI GAAGAC 1 cut(s) 2909
BpmI CTGGAG 3 cut(s) 1456, 2142, 2772
Bpu10I CCTNAGC 1 cut(s) 2469
Bpu1102I GCTNAGC 2 cut(s) 323, 2777
Bpu14I TTCGAA 1 cut(s) 1106
BpuEI CTTGAG 3 cut(s) 758, 774, 1778
BpuMI CCSGG 2 cut(s) 278, 279
Bsa29I ATCGAT 1 cut(s) 441
BsaBI GATNNNNATC 2 cut(s) 1983, 2802
BsaHI GRCGYC 2 cut(s) 351, 363
BsaI GGTCTC 2 cut(s) 1724, 1928
BsaXI ACNNNNNCTCC 8 cut(s) 107, 137, 167, 197, 996, 1026, 1605, 1635
Bsc4I CCNNNNNNNGG 8 cut(s) 178, 193, 318, 521, 1145, 1289, 1290, 2387
Bse118I RCCGGY 3 cut(s) 161, 742, 2701
Bse1I ACTGG 5 cut(s) 1473, 1782, 2352, 2789, 2921
Bse3DI GCAATG 2 cut(s) 532, 620
Bse8I GATNNNNATC 2 cut(s) 1983, 2802
BseBI CCWGG 7 cut(s) 77, 319, 395, 400, 761, 1245, 1487
BseCI ATCGAT 1 cut(s) 441
BseJI GATNNNNATC 2 cut(s) 1983, 2802
BseLI CCNNNNNNNGG 8 cut(s) 178, 193, 318, 521, 1145, 1289, 1290, 2387
BseMI GCAATG 2 cut(s) 532, 620
BseMII CTCAG 5 cut(s) 337, 2061, 2211, 2447, 2460
BseNI ACTGG 5 cut(s) 1473, 1782, 2352, 2789, 2921
BseRI GAGGAG 8 cut(s) 179, 209, 217, 797, 1626, 2176, 2197, 2855
BseSI GKGCMC 1 cut(s) 2119
BseX3I CGGCCG 2 cut(s) 163, 713
BseYI CCCAGC 1 cut(s) 460
BsgI GTGCAG 1 cut(s) 2960
Bsh1236I CGCG 3 cut(s) 207, 335, 2202
Bsh1285I CGRYCG 2 cut(s) 166, 716
BshVI ATCGAT 1 cut(s) 441
BsiEI CGRYCG 2 cut(s) 166, 716
BsiHKAI GWGCWC 3 cut(s) 227, 2196, 2767
BsiHKCI CYCGRG 3 cut(s) 191, 277, 840
BsiSI CCGG 5 cut(s) 126, 162, 278, 743, 2702
BslFI GGGAC 1 cut(s) 693
BslI CCNNNNNNNGG 8 cut(s) 178, 193, 318, 521, 1145, 1289, 1290, 2387
BsmAI GTCTC 5 cut(s) 310, 985, 1724, 1928, 2045
BsmFI GGGAC 1 cut(s) 693
BsmI GAATGC 1 cut(s) 1330
Bso31I GGTCTC 2 cut(s) 1724, 1928
BsoBI CYCGRG 3 cut(s) 191, 277, 840
Bsp119I TTCGAA 1 cut(s) 1106
Bsp1286I GDGCHC 6 cut(s) 227, 473, 516, 2119, 2196, 2767
Bsp1407I TGTACA 1 cut(s) 2686
Bsp1720I GCTNAGC 2 cut(s) 323, 2777
BspCNI CTCAG 5 cut(s) 336, 2062, 2212, 2446, 2461
BspDI ATCGAT 1 cut(s) 441
BspFNI CGCG 3 cut(s) 207, 335, 2202
BspHI TCATGA 2 cut(s) 508, 1344
BspLI GGNNCC 4 cut(s) 130, 642, 2206, 2805
BspMAI CTGCAG 3 cut(s) 299, 1534, 1636
BspPI GGATC 8 cut(s) 334, 410, 1229, 1274, 1520, 2117, 2798, 2811
BspQI GCTCTTC 2 cut(s) 215, 2772
BspT104I TTCGAA 1 cut(s) 1106
BspTNI GGTCTC 2 cut(s) 1724, 1928
BsrDI GCAATG 2 cut(s) 532, 620
BsrFI RCCGGY 3 cut(s) 161, 742, 2701
BsrGI TGTACA 1 cut(s) 2686
BsrI ACTGG 5 cut(s) 1473, 1782, 2352, 2789, 2921
BssAI RCCGGY 3 cut(s) 161, 742, 2701
BssNI GRCGYC 2 cut(s) 351, 363
BssT1I CCWWGG 6 cut(s) 644, 1411, 1866, 1906, 2126, 2964
Bst2UI CCWGG 7 cut(s) 77, 319, 395, 400, 761, 1245, 1487
Bst4CI ACNGT 6 cut(s) 265, 960, 1460, 1600, 1972, 2686
Bst6I CTCTTC 2 cut(s) 215, 2772
BstACI GRCGYC 2 cut(s) 351, 363
BstAPI GCANNNNNTGC 1 cut(s) 2300
BstAUI TGTACA 1 cut(s) 2686
BstBI TTCGAA 1 cut(s) 1106
BstC8I GCNNGC 6 cut(s) 159, 163, 381, 1383, 1966, 2946
BstDSI CCRYGG 3 cut(s) 91, 2200, 2549
BstEII GGTNACC 1 cut(s) 854
BstENI CCTNNNNNAGG 1 cut(s) 191
BstFNI CGCG 3 cut(s) 207, 335, 2202
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 5 cut(s) 204, 209, 335, 393, 1381
BstMAI GTCTC 5 cut(s) 310, 985, 1724, 1928, 2045
BstMCI CGRYCG 2 cut(s) 166, 716
BstNI CCWGG 7 cut(s) 77, 319, 395, 400, 761, 1245, 1487
BstNSI RCATGY 6 cut(s) 70, 1370, 1622, 1968, 2693, 2948
BstPI GGTNACC 1 cut(s) 854
BstSCI CCNGG 9 cut(s) 75, 276, 277, 317, 393, 398, 759, 1243, 1485
BstSFI CTRYAG 5 cut(s) 295, 1225, 1530, 1632, 1971
BstSLI GKGCMC 1 cut(s) 2119
BstUI CGCG 3 cut(s) 207, 335, 2202
BstV2I GAAGAC 1 cut(s) 2909
BstX2I RGATCY 3 cut(s) 1221, 2109, 2803
BstYI RGATCY 3 cut(s) 1221, 2109, 2803
BstZI CGGCCG 2 cut(s) 163, 713
Bsu15I ATCGAT 1 cut(s) 441
BsuTUI ATCGAT 1 cut(s) 441
BtgI CCRYGG 3 cut(s) 91, 2200, 2549
BtgZI GCGATG 1 cut(s) 217
BtrI CACGTC 1 cut(s) 85
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 5 cut(s) 244, 1968, 2682, 2782, 2928
Cac8I GCNNGC 6 cut(s) 159, 163, 381, 1383, 1966, 2946
CaiI CAGNNNCTG 2 cut(s) 2267, 2360
CciI TCATGA 2 cut(s) 508, 1344
CfoI GCGC 5 cut(s) 204, 209, 335, 393, 1381
Cfr10I RCCGGY 3 cut(s) 161, 742, 2701
Cfr13I GGNCC 9 cut(s) 79, 280, 641, 1351, 1840, 2123, 2138, 2363, 2961
Cfr42I CCGCGG 1 cut(s) 2203
Cfr9I CCCGGG 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 441
CseI GACGC 3 cut(s) 61, 352, 359
Csp6I GTAC 8 cut(s) 612, 757, 1025, 1643, 2178, 2569, 2687, 2816
CviQI GTAC 8 cut(s) 612, 757, 1025, 1643, 2178, 2569, 2687, 2816
DraI TTTAAA 3 cut(s) 1726, 1950, 2649
DraIII CACNNNGTG 1 cut(s) 420
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 3 cut(s) 3, 163, 713
EagI CGGCCG 2 cut(s) 163, 713
Eam1104I CTCTTC 2 cut(s) 215, 2772
EarI CTCTTC 2 cut(s) 215, 2772
EciI GGCGGA 3 cut(s) 230, 1052, 2650
Ecl136II GAGCTC 1 cut(s) 225
EclXI CGGCCG 2 cut(s) 163, 713
Eco130I CCWWGG 6 cut(s) 644, 1411, 1866, 1906, 2126, 2964
Eco147I AGGCCT 3 cut(s) 398, 1078, 1410
Eco24I GRGCYC 3 cut(s) 227, 473, 516
Eco31I GGTCTC 2 cut(s) 1724, 1928
Eco32I GATATC 1 cut(s) 1903
Eco47I GGWCC 3 cut(s) 1840, 2363, 2961
Eco52I CGGCCG 2 cut(s) 163, 713
Eco53kI GAGCTC 1 cut(s) 225
Eco57I CTGAAG 5 cut(s) 1101, 1865, 2262, 2310, 2325
Eco88I CYCGRG 3 cut(s) 191, 277, 840
Eco91I GGTNACC 1 cut(s) 854
EcoICRI GAGCTC 1 cut(s) 225
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 3 cut(s) 641, 1840, 2961
EcoO65I GGTNACC 1 cut(s) 854
EcoRII CCWGG 7 cut(s) 75, 317, 393, 398, 759, 1243, 1485
EcoRV GATATC 1 cut(s) 1903
EcoT14I CCWWGG 6 cut(s) 644, 1411, 1866, 1906, 2126, 2964
EcoT22I ATGCAT 2 cut(s) 622, 1661
EcoT38I GRGCYC 3 cut(s) 227, 473, 516
ErhI CCWWGG 6 cut(s) 644, 1411, 1866, 1906, 2126, 2964
FalI AAGNNNNNCTT 4 cut(s) 1133, 1165, 1632, 1664
FaqI GGGAC 1 cut(s) 693
FauI CCCGC 1 cut(s) 688
FbaI TGATCA 2 cut(s) 429, 2583
FblI GTMKAC 2 cut(s) 87, 348
FriOI GRGCYC 3 cut(s) 227, 473, 516
FspBI CTAG 6 cut(s) 344, 1074, 1608, 1895, 2043, 2859
GlaI GCGC 5 cut(s) 203, 208, 334, 392, 1380
GsaI CCCAGC 1 cut(s) 464
GsuI CTGGAG 3 cut(s) 1456, 2142, 2772
HaeII RGCGCY 1 cut(s) 205
HapII CCGG 5 cut(s) 126, 162, 278, 743, 2702
HgaI GACGC 3 cut(s) 61, 352, 359
HhaI GCGC 5 cut(s) 204, 209, 335, 393, 1381
Hin1I GRCGYC 2 cut(s) 351, 363
Hin6I GCGC 5 cut(s) 202, 207, 333, 391, 1379
HinP1I GCGC 5 cut(s) 202, 207, 333, 391, 1379
HincII GTYRAC 2 cut(s) 88, 349
HindII GTYRAC 2 cut(s) 88, 349
HindIII AAGCTT 3 cut(s) 1062, 2409, 2713
HinfI GANTC 9 cut(s) 32, 182, 272, 886, 1070, 1295, 1748, 1984, 2183
HpaII CCGG 5 cut(s) 126, 162, 278, 743, 2702
Hpy166II GTNNAC 7 cut(s) 88, 349, 367, 757, 1559, 2682, 2745
Hpy188III TCNNGA 9 cut(s) 406, 509, 842, 916, 1345, 1649, 1843, 2159, 2795
Hpy8I GTNNAC 7 cut(s) 88, 349, 367, 757, 1559, 2682, 2745
Hpy99I CGWCG 4 cut(s) 89, 332, 353, 359
HpyCH4III ACNGT 6 cut(s) 265, 960, 1460, 1600, 1972, 2686
HpyCH4IV ACGT 3 cut(s) 84, 818, 2008
HpySE526I ACGT 3 cut(s) 84, 818, 2008
Hsp92I GRCGYC 2 cut(s) 351, 363
HspAI GCGC 5 cut(s) 202, 207, 333, 391, 1379
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
Ksp22I TGATCA 2 cut(s) 429, 2583
KspI CCGCGG 1 cut(s) 2203
LguI GCTCTTC 2 cut(s) 215, 2772
LweI GCATC 7 cut(s) 150, 469, 1006, 1646, 1969, 2230, 2506
MaeI CTAG 6 cut(s) 344, 1074, 1608, 1895, 2043, 2859
MaeII ACGT 3 cut(s) 84, 818, 2008
MaeIII GTNAC 7 cut(s) 259, 854, 978, 1594, 1804, 2284, 2372
MboII GAAGA 9 cut(s) 232, 511, 679, 682, 1229, 2268, 2476, 2759, 2909
MfeI CAATTG 3 cut(s) 1512, 1989, 2600
MflI RGATCY 3 cut(s) 1221, 2109, 2803
MhlI GDGCHC 6 cut(s) 227, 473, 516, 2119, 2196, 2767
MlyI GAGTC 4 cut(s) 176, 266, 895, 2177
MmeI TCCRAC 1 cut(s) 156
Mph1103I ATGCAT 2 cut(s) 622, 1661
MroNI GCCGGC 1 cut(s) 161
MroXI GAANNNNTTC 2 cut(s) 1620, 2316
MseI TTAA 9 cut(s) 654, 822, 1286, 1725, 1766, 1949, 2103, 2511, 2648
MslI CAYNNNNRTG 2 cut(s) 215, 2694
MspA1I CMGCKG 4 cut(s) 138, 2202, 2264, 2360
MspI CCGG 5 cut(s) 126, 162, 278, 743, 2702
MspR9I CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 761, 1245, 1487
MssI GTTTAAAC 1 cut(s) 1950
MteI GCGCNGCGC 1 cut(s) 205
MunI CAATTG 3 cut(s) 1512, 1989, 2600
Mva1269I GAATGC 1 cut(s) 1330
MvaI CCWGG 7 cut(s) 77, 319, 395, 400, 761, 1245, 1487
MvnI CGCG 3 cut(s) 207, 335, 2202
NaeI GCCGGC 1 cut(s) 163
NciI CCSGG 2 cut(s) 278, 279
NgoMIV GCCGGC 1 cut(s) 161
NlaIV GGNNCC 4 cut(s) 130, 642, 2206, 2805
NmeAIII GCCGAG 3 cut(s) 481, 607, 691
NmuCI GTSAC 5 cut(s) 259, 854, 1594, 1804, 2372
NsiI ATGCAT 2 cut(s) 622, 1661
NspI RCATGY 6 cut(s) 70, 1370, 1622, 1968, 2693, 2948
NspV TTCGAA 1 cut(s) 1106
PaeI GCATGC 2 cut(s) 1968, 2948
PaeR7I CTCGAG 1 cut(s) 191
PagI TCATGA 2 cut(s) 508, 1344
PceI AGGCCT 3 cut(s) 398, 1078, 1410
PciI ACATGT 2 cut(s) 66, 1618
PciSI GCTCTTC 2 cut(s) 215, 2772
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 1330
PdiI GCCGGC 1 cut(s) 163
PdmI GAANNNNTTC 2 cut(s) 1620, 2316
PfeI GAWTC 5 cut(s) 32, 1070, 1295, 1748, 1984
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 1145
PleI GAGTC 4 cut(s) 176, 266, 894, 2177
PmeI GTTTAAAC 1 cut(s) 1950
PpsI GAGTC 4 cut(s) 176, 266, 894, 2177
PpuMI RGGWCCY 2 cut(s) 1840, 2961
PscI ACATGT 2 cut(s) 66, 1618
Psp124BI GAGCTC 1 cut(s) 227
Psp1406I AACGTT 1 cut(s) 818
Psp5II RGGWCCY 2 cut(s) 1840, 2961
Psp6I CCWGG 7 cut(s) 75, 317, 393, 398, 759, 1243, 1485
PspEI GGTNACC 1 cut(s) 854
PspFI CCCAGC 1 cut(s) 460
PspGI CCWGG 7 cut(s) 75, 317, 393, 398, 759, 1243, 1485
PspN4I GGNNCC 4 cut(s) 130, 642, 2206, 2805
PspPI GGNCC 9 cut(s) 79, 280, 641, 1351, 1840, 2123, 2138, 2363, 2961
PspPPI RGGWCCY 2 cut(s) 1840, 2961
PspXI VCTCGAGB 1 cut(s) 191
PstI CTGCAG 3 cut(s) 299, 1534, 1636
PstNI CAGNNNCTG 2 cut(s) 2267, 2360
PsuI RGATCY 3 cut(s) 1221, 2109, 2803
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 2 cut(s) 2264, 2360
RsaI GTAC 8 cut(s) 613, 758, 1026, 1644, 2179, 2570, 2688, 2817
RsaNI GTAC 8 cut(s) 612, 757, 1025, 1643, 2178, 2569, 2687, 2816
RseI CAYNNNNRTG 2 cut(s) 215, 2694
SacI GAGCTC 1 cut(s) 227
SacII CCGCGG 1 cut(s) 2203
SalI GTCGAC 2 cut(s) 86, 347
SapI GCTCTTC 2 cut(s) 215, 2772
SaqAI TTAA 9 cut(s) 654, 822, 1286, 1725, 1766, 1949, 2103, 2511, 2648
Sau96I GGNCC 9 cut(s) 79, 280, 641, 1351, 1840, 2123, 2138, 2363, 2961
ScaI AGTACT 1 cut(s) 613
SchI GAGTC 4 cut(s) 176, 266, 895, 2177
ScrFI CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 761, 1245, 1487
SduI GDGCHC 6 cut(s) 227, 473, 516, 2119, 2196, 2767
SfaNI GCATC 7 cut(s) 150, 469, 1006, 1646, 1969, 2230, 2506
SfcI CTRYAG 5 cut(s) 295, 1225, 1530, 1632, 1971
Sfr274I CTCGAG 1 cut(s) 191
Sfr303I CCGCGG 1 cut(s) 2203
SfuI TTCGAA 1 cut(s) 1106
SgrBI CCGCGG 1 cut(s) 2203
SinI GGWCC 3 cut(s) 1840, 2363, 2961
SlaI CTCGAG 1 cut(s) 191
SmaI CCCGGG 1 cut(s) 279
SmiMI CAYNNNNRTG 2 cut(s) 215, 2694
SmlI CTYRAG 4 cut(s) 191, 737, 789, 1757
SmoI CTYRAG 4 cut(s) 191, 737, 789, 1757
SphI GCATGC 2 cut(s) 1968, 2948
SseBI AGGCCT 3 cut(s) 398, 1078, 1410
SspMI CTAG 6 cut(s) 344, 1074, 1608, 1895, 2043, 2859
SstI GAGCTC 1 cut(s) 227
StuI AGGCCT 3 cut(s) 398, 1078, 1410
StyD4I CCNGG 9 cut(s) 75, 276, 277, 317, 393, 398, 759, 1243, 1485
StyI CCWWGG 6 cut(s) 644, 1411, 1866, 1906, 2126, 2964
TaaI ACNGT 6 cut(s) 265, 960, 1460, 1600, 1972, 2686
TaiI ACGT 3 cut(s) 87, 821, 2011
TaqI TCGA 8 cut(s) 87, 192, 270, 348, 357, 405, 441, 1106
TatI WGTACW 5 cut(s) 611, 1024, 1642, 2568, 2686
TauI GCSGC 6 cut(s) 141, 168, 207, 317, 767, 2205
TfiI GAWTC 5 cut(s) 32, 1070, 1295, 1748, 1984
Tru1I TTAA 9 cut(s) 654, 822, 1286, 1725, 1766, 1949, 2103, 2511, 2648
Tru9I TTAA 9 cut(s) 654, 822, 1286, 1725, 1766, 1949, 2103, 2511, 2648
TscAI CASTG 5 cut(s) 251, 1975, 2689, 2789, 2928
TseFI GTSAC 5 cut(s) 259, 854, 1594, 1804, 2372
Tsp45I GTSAC 5 cut(s) 259, 854, 1594, 1804, 2372
TspDTI ATGAA 9 cut(s) 233, 497, 1197, 1434, 1565, 1761, 1893, 2180, 2309
TspGWI ACGGA 2 cut(s) 247, 2840
TspMI CCCGGG 1 cut(s) 277
TspRI CASTG 5 cut(s) 251, 1975, 2689, 2789, 2928
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 1145
VpaK11BI GGWCC 3 cut(s) 1840, 2363, 2961
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 3 cut(s) 599, 1254, 1357
XceI RCATGY 6 cut(s) 70, 1370, 1622, 1968, 2693, 2948
XhoI CTCGAG 1 cut(s) 191
XmaI CCCGGG 1 cut(s) 277
XmiI GTMKAC 2 cut(s) 87, 348
XmnI GAANNNNTTC 2 cut(s) 1620, 2316
XspI CTAG 6 cut(s) 344, 1074, 1608, 1895, 2043, 2859
ZrmI AGTACT 1 cut(s) 613
Zsp2I ATGCAT 2 cut(s) 622, 1661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.