Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr10

Orthogroup ID

OG0002828

Physical Location & Seq

Genomic Coordinates Reverse (-)

21154579 .. 21159331

Gene Structure 4753 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom10g18000.2

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3231 bp

ATGGCCGATAACACCCGAAACATCCGAAACATATGCATACTGGCGCACGTGGATCACGGCAAGACCACGCTCGCCGACCACCTAATCGCCGGAACCGGCTCCGGCGTCGTCCACCCGAAACTCGCCGGGCGCCTCCGGTTCATGGACTATCTCGACGAGGAACAGAGGCGGGCCATCACCATGAAAAGCTCCTCGATTGCGCTCCACTACAAAGACCACTCCATCAATCTCATCGACTCCCCGGGCCACATGGACTTCTGCAGCGAGGTCTCCACCGCCGCCCGATTGAGCGACGGCGCTCTGGTCCTGGTCGACGCCGTCGAGGGCGTCCACATCCAGACGCACGCCGTGTTGCGCCAGGCCTGGATTGAGAAGCTGACGCCGTGCTTGGTGCTGAACAAGATCGACCGGCTGATCTCCGAGCTGAAATTGAGCCCCATAGAAGCTTACACGAGGTTAGTGCGTATTGTTCATGAGGTTAATTGGATTGTGAGTGCGTATAAGAGCGAGAAATACTTGTCCGACGTGGATGCTATACTTTCTGGGCCAGCGGGGGATGTGGGTAGTGATGAAAATCTGAGCTTTTTGGATGTGGAGGATGATGAGGAAGATACGTTTCAACCCCAGAAAGGGAATGTGGCGTTTGTGTGTGCTTTGGACGGTTGGGGTTTCTGCATTAGTGAGTTTGCTGAGATTTACGCTTCAAAATTTGGGGTGAGTGCGGCAGCATTGACAAAGGCTTTGTGGGGACCTAGGTACTTTAATCCGAAAACCAAGATGATTGTGGGGAAGAAAGGTGTAGCTGGTATGAAAAATGCTAGGCCAATGTTTGTTCAGTTTGTGCTTGAGCCGCTCTGGCAGGTTTATCAGGCCGCTTTGGACGATGATGGCGGTGGGAAAGTGATGCTTGAGAAAGTTATTAAGTCCTTTAATTTGAATGTGCCGCCTCGTGAGCTTCAGAACAAGGATCAGAAGGTTGTGCTTCAAGCAGTGATGAGTCGTTGGCTTCCGCTTGCAGATGCTGTTTTGTGGATGGTTGTGAGGTGTATGCCTGACCCGGTTGCAGCACAGGCGTTTAGGATAGGGCGGTTGCTTCCAAAGAGACAAGTTTTGAGTGATGGGGTTGATTCGGATGCGCTTGCAGAGGCGGAGCTTGTGAGGAAATCCGTTGAAGCTTGTGATTCGAGCCCTGAAGCTCCCTGTGTTGCTTTTGTATCCAAAATGTTTGCTGTCCCTATGAAAGTACTTCCACATAGGGGATTAGATGGGGAAATTGAAAACAACGTAAGTGATGAGGGTGAATTGAATGAGTGTTTCCTTGCATTTGCAAGGATCTTTAGTGGGGTTCTTTATTCAGGACAGAAAATTTTTGTCCTTTCGGCTATGGATGATCCATTAAAAGGGGAGTCAGTGAAAAAGCATTTGCAGGTGGCTGAGTTGCAATCTCTGTATCTCATGATGGGCCAAGGCTTAACACATGTGGCCTCTGCCCATGCCGGGAATCTTGTGGCAATTCGAGGCCTTGGCCAGCATATACTGAAAAGTGCAACACTTTCATCGACAAAAAACTGTTGGCCTTTCTCCAGTATGGCATTCCAGATTGCTCCTACTCTAAGAGTCGCAATTGAGCCAACTCATCCTGCAGATATGGGTGCACTGACTAAGGGTTTACGGCTGCTGAACCGGGCTGACCCGTTTGTTGGGGTAACAGTTTCTGATAGGGGAGAAAATGTGCTGTCTGCTGCAGGAGAGGTGCATCTTGAAAGATGCATAAAAGATTTGAAGGAGAGATTTGCAAGGGTAAGCCTGGAAGTGTCTCCACCTCTTGTGTCTTATAAAGAGACCATTGAGGGTAATGTAGCCGACAAACTTGAGAATTTGAAATTCTTTCGTACAAGCTCAGATTATGTTGAGAAAAAGACAGCAAACGGTAGGTGTACGATAAAGGTGCAAGTCATAAAGCTCCCACCCTCTTTAACTAAGGTGCTTGAAGATAGTTCTGATTTACTTGGAGATATCCTTGGAGGTAGAGCTTCTCAGACCAGTAAAAGTTTTGACACAGAGATTTCAAGGATTGCAGAAGATGACAACCCGATTGAAGCACTGAAGAAACGTATCATGGATGCTGTGGAGAGTGATATTCTGTCTAGTGGTGATGATGACAAGCATCGGGTTGAGAAGTGTAAACTAAAGTGGCAAAAATTACTTAAAAGGATATGGGCGCTTGGCCCTTCGCAAGTAGGTCCTAATATCCTCCTTACTCCAGACCTCAAAGGAAAGGGTTCAGATGGCTCTGTCCTCATCTGTGGTTCCTCTCACGTATCTCAAAAATTAGGGTTTGTGGATGCTTCTGGCATTGGCAATACACCTGCTGATACATCTTCAGAAGTAGCTCAAGCATTGTTAGTTGAGGCGGAGAGCCTTGAAAGCAGTGTAGTATCTGGGTTTCAAGTGGCGACAGCAGCTGGACCTTTATGCGATGAACCTATGCGGGGTTTAGCCTTTATTATCGAGGCTAAAATTGAACCATTGATGGCTCAGTCTGATGAAGGGGAAGCCTCCCACCACCAACCTGAGCAATATGGTATCTTCAGAGGACAGGTAATGACAACCATCAAAGATGCTTGTAGAGAAGCTGTACTTCAAAAGAAGCCACGGCTTGTGGAGGCCATGTACTTCTGTGAGTTGAATACGTCAACTGAGCATTTGGGTTCTATGTACGCTGTGCTTGGGCGAAGGCGGGCTAGAGTTTTAAAGGAGGAAATGCAGGAAGGCTCCCCATTGTTCACTGTGCATGCATATGTGCCAGTTTCTGAAAGCTTTGGTTTTGCAGATGAGCTTAGAAGATGGACTGCTGGTGCTGCAAGTGCTCTTCTTGTGCTTAGTCACTGGGAAGCACTTCCAGAAGATCCTTTCTTTGTACCAAAAACAGAAGAAGAAATTGAGGAGTTTGGTGACGGGTCTAGTATGCTTCCCAATACTGCAAGGAAACTAATTAACGCCGTAAGACGGAAGAAGGGCCTTCCTGTAGAGGAAAAAGTAGTACAGCACGCGACCAAGCAGAGGACCCTGGCTCGAACTTCATCTAAACGTCATGATGATGATAATGATGATGGTGACGACGACGACGATAGTGATGACGATGACGATGATGATGGCGACGAAGACTTGGAACGCAAGAAAGAGAGAGAAAGTGGAAGAGATCAAGTGGTTGTTGCCTGCGCCACATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko03008 map03008

Pfam Domains

Protein Families

EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

1077

Amino Acids

118.12

Weight (kDa)

5.36

Isoelectric Point (pI)

42.6

Instability Index

Protein Domains (Pfam)

No Pfam domains detected for this protein.

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0002828)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G22980 AT3G22980 AT3G22980
fragaria_vesca	FvH4_3g14510 FvH4_4g04790 FvH4_4g04800 FvH4_4g04801 FvH4_5g27362 FvH4_6g08980
malus_domestica	MD10G1213300.v1.1
prunus_persica	Prupe.4G129900_v2.0.a1
pyrus_communis	pycom05g21320 pycom05g21330 pycom05g23810 pycom10g18000
rosa_chinensis	RchiOBHm_Chr6g0274771
rosa_laevigata	RLG00000018664 RLG00000032745
rosa_multiflora	Rmu_co8270721.1_g000001 Rmu_co8337679.1_g000001 Rmu_sc0001938.1_g000004 Rmu_sc0006223.1_g000002 Rmu_sc0014273.1_g000001 Rmu_ssc0000027.1_g000001
rosa_roxburghii	Rroxscaffold_1G00055330 Rroxscaffold_2G00121910 Rroxscaffold_2G00121920 Rroxscaffold_7G00193950
rosa_rugosa	Rorug06G0084300
rosa_samantha	Rh2AG294600 Rh2DG318500 Rh2DG318700 Rh5DG169500 Rh6AG200200 Rh6BG203700 Rh6CG203900 Rh6DG195300
rosa_wichuraiana	Rw5G015390 Rw6G017390

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1836
AarI	CACCTGC	2 cut(s)	1417, 2377
Acc36I	ACCTGC	3 cut(s)	850, 1417, 2377
AccB1I	GGYRCC	1 cut(s)	129
AccBSI	CCGCTC	1 cut(s)	853
AccI	GTMKAC	1 cut(s)	312
AccII	CGCG	1 cut(s)	3053
AclWI	GGATC	5 cut(s)	60, 975, 1340, 1385, 2903
AcoI	YGGCCR	2 cut(s)	3, 1525
AcsI	RAATTY	4 cut(s)	707, 1365, 1876, 1883
AcuI	CTGAAG	5 cut(s)	941, 1212, 2126, 2367, 2575
AcvI	CACGTG	1 cut(s)	49
AcyI	GRCGYC	5 cut(s)	105, 130, 315, 327, 380
AdeI	CACNNNGTG	1 cut(s)	349
AfaI	GTAC	9 cut(s)	758, 1245, 1894, 1939, 2640, 2675, 2720, 2922, 3045
AflIII	ACRYGT	1 cut(s)	1477
AjiI	CACGTC	1 cut(s)	526
AjnI	CCWGG	5 cut(s)	306, 357, 362, 1806, 3069
Alw21I	GWGCWC	2 cut(s)	1657, 2872
Alw26I	GTCTC	4 cut(s)	274, 1096, 1821, 1835
Alw44I	GTGCAC	1 cut(s)	1653
AlwI	GGATC	5 cut(s)	60, 975, 1340, 1385, 2903
AlwNI	CAGNNNCTG	4 cut(s)	1022, 1715, 2465, 2813
Ama87I	CYCGRG	1 cut(s)	241
ApaLI	GTGCAC	1 cut(s)	1653
ApeKI	GCWGC	7 cut(s)	261, 725, 1064, 1675, 1742, 2462, 2861
ApoI	RAATTY	4 cut(s)	707, 1365, 1876, 1883
ArsI	GACNNNNNNTTYG	2 cut(s)	724, 756
Asp700I	GAANNNNTTC	2 cut(s)	2281, 2898
AspA2I	CCTAGG	1 cut(s)	752
AspLEI	GCGC	8 cut(s)	46, 132, 202, 299, 357, 1138, 2224, 3224
AsuC2I	CCSGG	6 cut(s)	127, 242, 243, 1058, 1498, 1685
AsuHPI	GGTGA	6 cut(s)	169, 727, 1310, 2165, 2966, 3128
AvaI	CYCGRG	1 cut(s)	241
AvaII	GGWCC	5 cut(s)	304, 749, 2243, 2468, 3066
AvrII	CCTAGG	1 cut(s)	752
BaeGI	GKGCMC	1 cut(s)	1657
BalI	TGGCCA	1 cut(s)	1527
BanI	GGYRCC	1 cut(s)	129
BanII	GRGCYC	2 cut(s)	437, 1190
BauI	CACGAG	2 cut(s)	451, 948
BbrPI	CACGTG	1 cut(s)	49
BbsI	GAAGAC	1 cut(s)	3171
Bbv12I	GWGCWC	2 cut(s)	1657, 2872
BbvI	GCAGC	7 cut(s)	273, 737, 1076, 1662, 1729, 2474, 2848
BceAI	ACGGC	8 cut(s)	73, 302, 310, 332, 367, 1688, 2672, 2987
BcgI	CGANNNNNNTGC	8 cut(s)	15, 49, 442, 476, 512, 546, 1930, 1964
BciT130I	CCWGG	5 cut(s)	308, 359, 364, 1808, 3071
BciVI	GTATCC	1 cut(s)	1225
BcnI	CCSGG	6 cut(s)	127, 242, 243, 1058, 1498, 1685
BcoDI	GTCTC	4 cut(s)	274, 1096, 1821, 1835
BfaI	CTAG	5 cut(s)	753, 819, 2148, 2745, 2964
BfmI	CTRYAG	4 cut(s)	259, 1641, 1743, 3027
BfoI	RGCGCY	3 cut(s)	133, 300, 2225
BfuAI	ACCTGC	3 cut(s)	850, 1417, 2377
BfuI	GTATCC	1 cut(s)	1225
BglI	GCCNNNNNGGC	1 cut(s)	856
BlnI	CCTAGG	1 cut(s)	752
BmcAI	AGTACT	1 cut(s)	1245
Bme18I	GGWCC	5 cut(s)	304, 749, 2243, 2468, 3066
BmeT110I	CYCGRG	1 cut(s)	241
BmgBI	CACGTC	1 cut(s)	526
BmiI	GGNNCC	7 cut(s)	94, 100, 131, 750, 2311, 2776, 3068
BmrI	ACTGGG	1 cut(s)	2899
BmuI	ACTGGG	1 cut(s)	2899
BpiI	GAAGAC	1 cut(s)	3171
BpmI	CTGGAG	2 cut(s)	1567, 2247
Bpu10I	CCTNAGC	1 cut(s)	2574
BpuEI	CTTGAG	4 cut(s)	866, 929, 1892, 2379
BpuMI	CCSGG	6 cut(s)	127, 242, 243, 1058, 1498, 1685
BsaAI	YACGTR	2 cut(s)	49, 2320
BsaBI	GATNNNNATC	1 cut(s)	573
BsaHI	GRCGYC	5 cut(s)	105, 130, 315, 327, 380
BsaI	GGTCTC	2 cut(s)	274, 1835
BsaJI	CCNNGG	8 cut(s)	240, 241, 752, 1465, 1522, 2020, 2654, 3069
BsaWI	WCCGGW	1 cut(s)	135
BsaXI	ACNNNNNCTCC	4 cut(s)	1716, 1746, 2657, 2687
Bse118I	RCCGGY	2 cut(s)	95, 408
Bse1I	ACTGG	5 cut(s)	45, 1584, 2043, 2807, 2894
Bse8I	GATNNNNATC	1 cut(s)	573
BseBI	CCWGG	5 cut(s)	308, 359, 364, 1808, 3071
BseDI	CCNNGG	8 cut(s)	240, 241, 752, 1465, 1522, 2020, 2654, 3069
BseJI	GATNNNNATC	1 cut(s)	573
BseMII	CTCAG	8 cut(s)	569, 681, 1425, 1914, 2051, 2552, 2565, 2691
BseNI	ACTGG	5 cut(s)	45, 1584, 2043, 2807, 2894
BseRI	GAGGAG	2 cut(s)	181, 2960
BseSI	GKGCMC	1 cut(s)	1657
BseXI	GCAGC	7 cut(s)	273, 737, 1076, 1662, 1729, 2474, 2848
Bsh1236I	CGCG	1 cut(s)	3053
Bsh1285I	CGRYCG	1 cut(s)	409
BshNI	GGYRCC	1 cut(s)	129
BsiEI	CGRYCG	1 cut(s)	409
BsiHKAI	GWGCWC	2 cut(s)	1657, 2872
BsiHKCI	CYCGRG	1 cut(s)	241
BslFI	GGGAC	2 cut(s)	762, 1217
BsmAI	GTCTC	4 cut(s)	274, 1096, 1821, 1835
BsmFI	GGGAC	2 cut(s)	762, 1217
BsmI	GAATGC	1 cut(s)	1592
Bso31I	GGTCTC	2 cut(s)	274, 1835
BsoBI	CYCGRG	1 cut(s)	241
Bsp1286I	GDGCHC	4 cut(s)	437, 1190, 1657, 2872
Bsp143I	GATC	8 cut(s)	52, 402, 414, 967, 1332, 1390, 2908, 3202
BspCNI	CTCAG	8 cut(s)	570, 682, 1426, 1913, 2050, 2551, 2566, 2692
BspFNI	CGCG	1 cut(s)	3053
BspHI	TCATGA	3 cut(s)	472, 1455, 3094
BspLI	GGNNCC	7 cut(s)	94, 100, 131, 750, 2311, 2776, 3068
BspMAI	CTGCAG	3 cut(s)	263, 1645, 1747
BspMI	ACCTGC	3 cut(s)	850, 1417, 2377
BspPI	GGATC	5 cut(s)	60, 975, 1340, 1385, 2903
BspQI	GCTCTTC	1 cut(s)	2877
BspT107I	GGYRCC	1 cut(s)	129
BspTNI	GGTCTC	2 cut(s)	274, 1835
BsrBI	CCGCTC	1 cut(s)	853
BsrFI	RCCGGY	2 cut(s)	95, 408
BsrI	ACTGG	5 cut(s)	45, 1584, 2043, 2807, 2894
BssAI	RCCGGY	2 cut(s)	95, 408
BssECI	CCNNGG	8 cut(s)	240, 241, 752, 1465, 1522, 2020, 2654, 3069
BssMI	GATC	8 cut(s)	52, 402, 414, 967, 1332, 1390, 2908, 3202
BssNI	GRCGYC	5 cut(s)	105, 130, 315, 327, 380
BssSI	CACGAG	2 cut(s)	451, 948
BssT1I	CCWWGG	4 cut(s)	752, 1465, 1522, 2020
Bst2BI	CACGAG	2 cut(s)	451, 948
Bst2UI	CCWGG	5 cut(s)	308, 359, 364, 1808, 3071
Bst4CI	ACNGT	5 cut(s)	662, 1571, 1711, 1931, 2791
Bst6I	CTCTTC	2 cut(s)	2877, 3193
BstACI	GRCGYC	5 cut(s)	105, 130, 315, 327, 380
BstBAI	YACGTR	2 cut(s)	49, 2320
BstDSI	CCRYGG	1 cut(s)	2654
BstFNI	CGCG	1 cut(s)	3053
BstH2I	RGCGCY	3 cut(s)	133, 300, 2225
BstHHI	GCGC	8 cut(s)	46, 132, 202, 299, 357, 1138, 2224, 3224
BstKTI	GATC	8 cut(s)	55, 405, 417, 970, 1335, 1393, 2911, 3205
BstMAI	GTCTC	4 cut(s)	274, 1096, 1821, 1835
BstMBI	GATC	8 cut(s)	52, 402, 414, 967, 1332, 1390, 2908, 3202
BstMCI	CGRYCG	1 cut(s)	409
BstMWI	GCNNNNNNNGC	8 cut(s)	850, 856, 952, 1070, 2427, 2462, 2861, 2867
BstNI	CCWGG	5 cut(s)	308, 359, 364, 1808, 3071
BstNSI	RCATGY	2 cut(s)	1481, 2798
BstSFI	CTRYAG	4 cut(s)	259, 1641, 1743, 3027
BstSLI	GKGCMC	1 cut(s)	1657
BstUI	CGCG	1 cut(s)	3053
BstV1I	GCAGC	7 cut(s)	273, 737, 1076, 1662, 1729, 2474, 2848
BstV2I	GAAGAC	1 cut(s)	3171
BstX2I	RGATCY	2 cut(s)	1332, 2908
BstYI	RGATCY	2 cut(s)	1332, 2908
BsuI	GTATCC	1 cut(s)	1225
BtgI	CCRYGG	1 cut(s)	2654
BtgZI	GCGATG	1 cut(s)	2493
BtrI	CACGTC	1 cut(s)	526
BtsI	GCAGTG	2 cut(s)	996, 2437
BtsIMutI	CAGTG	7 cut(s)	996, 1416, 1655, 2102, 2437, 2787, 2887
BveI	ACCTGC	3 cut(s)	850, 1417, 2377
CaiI	CAGNNNCTG	4 cut(s)	1022, 1715, 2465, 2813
CciI	TCATGA	3 cut(s)	472, 1455, 3094
CfoI	GCGC	8 cut(s)	46, 132, 202, 299, 357, 1138, 2224, 3224
Cfr10I	RCCGGY	2 cut(s)	95, 408
Cfr9I	CCCGGG	1 cut(s)	241
CseI	GACGC	5 cut(s)	94, 316, 323, 349, 388
Csp6I	GTAC	9 cut(s)	757, 1244, 1893, 1938, 2639, 2674, 2719, 2921, 3044
CviQI	GTAC	9 cut(s)	757, 1244, 1893, 1938, 2639, 2674, 2719, 2921, 3044
DinI	GGCGCC	1 cut(s)	131
DpnI	GATC	8 cut(s)	54, 404, 416, 969, 1334, 1392, 2910, 3204
DpnII	GATC	8 cut(s)	52, 402, 414, 967, 1332, 1390, 2908, 3202
DraI	TTTAAA	1 cut(s)	2754
DraIII	CACNNNGTG	1 cut(s)	349
EaeI	YGGCCR	2 cut(s)	3, 1525
Eam1104I	CTCTTC	2 cut(s)	2877, 3193
EarI	CTCTTC	2 cut(s)	2877, 3193
EciI	GGCGGA	2 cut(s)	1163, 2429
Eco130I	CCWWGG	4 cut(s)	752, 1465, 1522, 2020
Eco147I	AGGCCT	2 cut(s)	362, 1521
Eco24I	GRGCYC	2 cut(s)	437, 1190
Eco31I	GGTCTC	2 cut(s)	274, 1835
Eco32I	GATATC	1 cut(s)	2017
Eco47I	GGWCC	5 cut(s)	304, 749, 2243, 2468, 3066
Eco57I	CTGAAG	5 cut(s)	941, 1212, 2126, 2367, 2575
Eco72I	CACGTG	1 cut(s)	49
Eco88I	CYCGRG	1 cut(s)	241
EcoO109I	RGGNCCY	4 cut(s)	749, 2243, 3019, 3066
EcoRII	CCWGG	5 cut(s)	306, 357, 362, 1806, 3069
EcoRV	GATATC	1 cut(s)	2017
EcoT14I	CCWWGG	4 cut(s)	752, 1465, 1522, 2020
EcoT22I	ATGCAT	3 cut(s)	38, 1772, 2800
EcoT38I	GRGCYC	2 cut(s)	437, 1190
EgeI	GGCGCC	1 cut(s)	131
EheI	GGCGCC	1 cut(s)	131
ErhI	CCWWGG	4 cut(s)	752, 1465, 1522, 2020
FalI	AAGNNNNNCTT	4 cut(s)	891, 923, 2625, 2657
FaqI	GGGAC	2 cut(s)	762, 1217
FauI	CCCGC	4 cut(s)	162, 544, 2484, 2733
FauNDI	CATATG	2 cut(s)	32, 2800
FblI	GTMKAC	1 cut(s)	312
FriOI	GRGCYC	2 cut(s)	437, 1190
FspBI	CTAG	5 cut(s)	753, 819, 2148, 2745, 2964
GlaI	GCGC	8 cut(s)	45, 131, 201, 298, 356, 1137, 2223, 3223
GsuI	CTGGAG	2 cut(s)	1567, 2247
HaeII	RGCGCY	3 cut(s)	133, 300, 2225
HgaI	GACGC	5 cut(s)	94, 316, 323, 349, 388
HhaI	GCGC	8 cut(s)	46, 132, 202, 299, 357, 1138, 2224, 3224
Hin1I	GRCGYC	5 cut(s)	105, 130, 315, 327, 380
Hin6I	GCGC	8 cut(s)	44, 130, 200, 297, 355, 1136, 2222, 3222
HinP1I	GCGC	8 cut(s)	44, 130, 200, 297, 355, 1136, 2222, 3222
HincII	GTYRAC	2 cut(s)	313, 2697
HindII	GTYRAC	2 cut(s)	313, 2697
HindIII	AAGCTT	3 cut(s)	444, 1173, 2818
HinfI	GANTC	7 cut(s)	236, 997, 1127, 1181, 1406, 1501, 1617
HphI	GGTGA	6 cut(s)	169, 727, 1310, 2165, 2966, 3128
Hpy166II	GTNNAC	9 cut(s)	112, 313, 331, 1655, 1670, 1938, 2186, 2697, 2787
Hpy8I	GTNNAC	9 cut(s)	112, 313, 331, 1655, 1670, 1938, 2186, 2697, 2787
HpyAV	CCTTC	8 cut(s)	967, 1777, 2241, 2543, 2730, 2765, 3010, 3032
HpyCH4III	ACNGT	5 cut(s)	662, 1571, 1711, 1931, 2791
HpyCH4IV	ACGT	8 cut(s)	48, 525, 614, 1284, 2113, 2319, 2693, 3091
HpyF10VI	GCNNNNNNNGC	8 cut(s)	850, 856, 952, 1070, 2427, 2462, 2861, 2867
HpySE526I	ACGT	8 cut(s)	48, 525, 614, 1284, 2113, 2319, 2693, 3091
Hsp92I	GRCGYC	5 cut(s)	105, 130, 315, 327, 380
HspAI	GCGC	8 cut(s)	44, 130, 200, 297, 355, 1136, 2222, 3222
KasI	GGCGCC	1 cut(s)	129
Kzo9I	GATC	8 cut(s)	52, 402, 414, 967, 1332, 1390, 2908, 3202
LguI	GCTCTTC	1 cut(s)	2877
LmnI	GCTCC	8 cut(s)	104, 194, 207, 1150, 1201, 1609, 1968, 2780
Lsp1109I	GCAGC	7 cut(s)	273, 737, 1076, 1662, 1729, 2474, 2848
MaeI	CTAG	5 cut(s)	753, 819, 2148, 2745, 2964
MaeII	ACGT	8 cut(s)	48, 525, 614, 1284, 2113, 2319, 2693, 3091
MaeIII	GTNAC	4 cut(s)	1705, 2885, 2954, 3116
MalI	GATC	8 cut(s)	54, 404, 416, 969, 1334, 1392, 2910, 3204
MbiI	CCGCTC	1 cut(s)	853
MboI	GATC	8 cut(s)	52, 402, 414, 967, 1332, 1390, 2908, 3202
MfeI	CAATTG	1 cut(s)	1623
MflI	RGATCY	2 cut(s)	1332, 2908
MhlI	GDGCHC	4 cut(s)	437, 1190, 1657, 2872
MlsI	TGGCCA	1 cut(s)	1527
MluNI	TGGCCA	1 cut(s)	1527
Mly113I	GGCGCC	1 cut(s)	130
MlyI	GAGTC	4 cut(s)	230, 1006, 1415, 1626
MmeI	TCCRAC	1 cut(s)	546
Mox20I	TGGCCA	1 cut(s)	1527
Mph1103I	ATGCAT	3 cut(s)	38, 1772, 2800
MroXI	GAANNNNTTC	2 cut(s)	2281, 2898
MscI	TGGCCA	1 cut(s)	1527
MslI	CAYNNNNRTG	3 cut(s)	179, 2799, 3099
Msp20I	TGGCCA	1 cut(s)	1527
MspA1I	CMGCKG	2 cut(s)	551, 2465
MunI	CAATTG	1 cut(s)	1623
Mva1269I	GAATGC	1 cut(s)	1592
MvaI	CCWGG	5 cut(s)	308, 359, 364, 1808, 3071
MvnI	CGCG	1 cut(s)	3053
MwoI	GCNNNNNNNGC	8 cut(s)	850, 856, 952, 1070, 2427, 2462, 2861, 2867
NarI	GGCGCC	1 cut(s)	130
NciI	CCSGG	6 cut(s)	127, 242, 243, 1058, 1498, 1685
NdeI	CATATG	2 cut(s)	32, 2800
NdeII	GATC	8 cut(s)	52, 402, 414, 967, 1332, 1390, 2908, 3202
NlaIV	GGNNCC	7 cut(s)	94, 100, 131, 750, 2311, 2776, 3068
NmuCI	GTSAC	3 cut(s)	2885, 2954, 3116
NsiI	ATGCAT	3 cut(s)	38, 1772, 2800
NspI	RCATGY	2 cut(s)	1481, 2798
PaeI	GCATGC	1 cut(s)	2798
PagI	TCATGA	3 cut(s)	472, 1455, 3094
PaqCI	CACCTGC	2 cut(s)	1417, 2377
PceI	AGGCCT	2 cut(s)	362, 1521
PciI	ACATGT	1 cut(s)	1477
PciSI	GCTCTTC	1 cut(s)	2877
PcsI	WCGNNNNNNNCGW	2 cut(s)	318, 3126
PctI	GAATGC	1 cut(s)	1592
PdmI	GAANNNNTTC	2 cut(s)	2281, 2898
PfeI	GAWTC	3 cut(s)	1127, 1181, 1501
PflFI	GACNNNGTC	1 cut(s)	317
PleI	GAGTC	4 cut(s)	230, 1005, 1414, 1625
PluTI	GGCGCC	1 cut(s)	133
PmaCI	CACGTG	1 cut(s)	49
PmlI	CACGTG	1 cut(s)	49
PpsI	GAGTC	4 cut(s)	230, 1005, 1414, 1625
Ppu21I	YACGTR	2 cut(s)	49, 2320
PpuMI	RGGWCCY	3 cut(s)	749, 2243, 3066
PscI	ACATGT	1 cut(s)	1477
PsiI	TTATAA	1 cut(s)	1836
Psp5II	RGGWCCY	3 cut(s)	749, 2243, 3066
Psp6I	CCWGG	5 cut(s)	306, 357, 362, 1806, 3069
PspCI	CACGTG	1 cut(s)	49
PspGI	CCWGG	5 cut(s)	306, 357, 362, 1806, 3069
PspN4I	GGNNCC	7 cut(s)	94, 100, 131, 750, 2311, 2776, 3068
PspPPI	RGGWCCY	3 cut(s)	749, 2243, 3066
PstI	CTGCAG	3 cut(s)	263, 1645, 1747
PstNI	CAGNNNCTG	4 cut(s)	1022, 1715, 2465, 2813
PsuI	RGATCY	2 cut(s)	1332, 2908
PsyI	GACNNNGTC	1 cut(s)	317
PvuII	CAGCTG	1 cut(s)	2465
RsaI	GTAC	9 cut(s)	758, 1245, 1894, 1939, 2640, 2675, 2720, 2922, 3045
RsaNI	GTAC	9 cut(s)	757, 1244, 1893, 1938, 2639, 2674, 2719, 2921, 3044
RseI	CAYNNNNRTG	3 cut(s)	179, 2799, 3099
SalI	GTCGAC	1 cut(s)	311
SapI	GCTCTTC	1 cut(s)	2877
Sau3AI	GATC	8 cut(s)	52, 402, 414, 967, 1332, 1390, 2908, 3202
ScaI	AGTACT	1 cut(s)	1245
SchI	GAGTC	4 cut(s)	230, 1006, 1415, 1626
SduI	GDGCHC	4 cut(s)	437, 1190, 1657, 2872
SfcI	CTRYAG	4 cut(s)	259, 1641, 1743, 3027
SfoI	GGCGCC	1 cut(s)	131
SinI	GGWCC	5 cut(s)	304, 749, 2243, 2468, 3066
SmaI	CCCGGG	1 cut(s)	243
SmiMI	CAYNNNNRTG	3 cut(s)	179, 2799, 3099
SmlI	CTYRAG	4 cut(s)	845, 908, 1871, 2394
SmoI	CTYRAG	4 cut(s)	845, 908, 1871, 2394
SphI	GCATGC	1 cut(s)	2798
SseBI	AGGCCT	2 cut(s)	362, 1521
SspDI	GGCGCC	1 cut(s)	129
SspMI	CTAG	5 cut(s)	753, 819, 2148, 2745, 2964
StuI	AGGCCT	2 cut(s)	362, 1521
StyI	CCWWGG	4 cut(s)	752, 1465, 1522, 2020
TaaI	ACNGT	5 cut(s)	662, 1571, 1711, 1931, 2791
TaiI	ACGT	8 cut(s)	51, 528, 617, 1287, 2116, 2322, 2696, 3094
TatI	WGTACW	4 cut(s)	1243, 2638, 2673, 3043
TauI	GCSGC	5 cut(s)	281, 725, 853, 875, 946
TfiI	GAWTC	3 cut(s)	1127, 1181, 1501
TscAI	CASTG	7 cut(s)	996, 1416, 1662, 2109, 2437, 2794, 2894
TseFI	GTSAC	3 cut(s)	2885, 2954, 3116
TseI	GCWGC	7 cut(s)	261, 725, 1064, 1675, 1742, 2462, 2861
Tsp45I	GTSAC	3 cut(s)	2885, 2954, 3116
TspGWI	ACGGA	2 cut(s)	1156, 3025
TspMI	CCCGGG	1 cut(s)	241
TspRI	CASTG	7 cut(s)	996, 1416, 1662, 2109, 2437, 2794, 2894
Tth111I	GACNNNGTC	1 cut(s)	317
VneI	GTGCAC	1 cut(s)	1653
VpaK11BI	GGWCC	5 cut(s)	304, 749, 2243, 2468, 3066
XapI	RAATTY	4 cut(s)	707, 1365, 1876, 1883
XceI	RCATGY	2 cut(s)	1481, 2798
XcmI	CCANNNNNNNNNTGG	1 cut(s)	781
XmaI	CCCGGG	1 cut(s)	241
XmaJI	CCTAGG	1 cut(s)	752
XmiI	GTMKAC	1 cut(s)	312
XmnI	GAANNNNTTC	2 cut(s)	2281, 2898
XspI	CTAG	5 cut(s)	753, 819, 2148, 2745, 2964
ZrmI	AGTACT	1 cut(s)	1245
Zsp2I	ATGCAT	3 cut(s)	38, 1772, 2800

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.