RLG00000018664

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0002828
Physical Location & Seq
Forward (+)
33461637 .. 33464768
3132 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018664

Sequence Viewer

Length: 3132 bp
ATGGCCGACCCGACCCAACAACCCGAACCCGAATCAACCACACCCGACACCCGAAAGCTCCGAAACATCTGCGTCCTGGCCCACGTTGACCACGGCAAGACGACCCTCTGCGACCACCTCATCTCCGGCTCCGACAGCGGCATCATCCACCCCAAGCTCGCCGGCCGCCTCCGTTACATGGACTCCCTCCTCGAGGAGCAGCGCCGCGCCATCACCATGAAGAGCTCCTCCATCGCCCTCCGCCACCGCGACCACTCCGTCACCGTCATCGACTCCCCGGGCCACATGGACTTCTGCAGCGAAGTCTCCACCGCCGCCAGGCTCAGCGACGGCGCGTTGATCCTAGTCGACGCCGTCGAGGGCGTCCACATCCAAACGCACGCCGTCTTGCGCCAGGCCTGGATCGAGAAGCTCACGCCGTGCTTGGTGATCAACAAGATCGATAGGTTGATCGCCGAGCTGGGAATGAGCCCTATGGATGCGTATGTAAAGTTGTTGAAAATTGTTCATGAGGTTAATAACATTGTTAGTGCTTTTAAATCTGTGAAATACTTGAACGATGTGGATGCCATTCTTTCGGGGCCGGCCGGCGCCGACGAGTTGGCGTTTGAGGAGATTGAGGATGATGAGGAGCTCATGTTTCAGCCCCAGAAGGGCAATGTGGCCTTTGTGTGTGCTTTGGATGGTTGGGGGTTTAGTATTAGGCAATTTGCCGAGTTTTATGAGGCGAAATTCGGGTGGAGTACTAATGCATTGCAGAAGGCTTTATGGGGGCCTTGGTATTTTAATCACAAAGAGAAGAAGATTGTGGGACAGAAGGGTGTGGCGGGTTTCAAAAAGGCTCGGCCGATGTTTGTTGAGTTTGTGCTTGAGCCGGTTTGGAGTGTGTACCAGGCGGCTTTGAAGGAGAAGGAGGAGGCTGAAGTGATGGTTCAGAAGGTGATTAGAACGTTTAATTTGACTATACCACCTCGGGATTTGAAGGGTGACCCAAAAGGTATGGTTCAGGCTGTGATGAGTCGGTGGCTTCCTCTGCATGAGGCTATCTTGAACATGGTTATTAGGTGTATGCCTGACCCGATTGCAGCACAGTCATTTCGGATTACAAGGTTACTTCCAAAGAGAGAAGTTGTGGGAGATGGGGTTGATGCTAATGTACTTGCAGAGGCGGAGCTTGTGAGAAGTTCGGTGGAAGCTTGTGATTCTAGGCCTGAAGCTCCTTGTGTTGTTTTTGTTTCAAAAATGTTTGCTGTGCCAACGAAAACACTTCCAAAGTACGGCTTGGATGGGGAGATTTTGGATAATACATCTGATGAAGGTGAATTGGATGAGTGCTTCCTTGCGTTTGCAAGGATCTATAGTGGGGTTCTTCGTCCTGGTGAGAAAATTTATGTGCTTTCAGCTTTATATGATCCCTTAAAAGGGGAATCAATGCAGAAGCATATTCAGGTGGCTAAGTTGCATTCTCTGTATCTCATGATGGGCCAAAATTTACAGCATGTGCCAGAGGCGCAGGCAGGGGATATTGTGGCAATCCGAGGCCTTGGGCAGCATTTACTGAAAACTGCAACACTTTCATCAACAAAGAACTGTTGGCCTTTCTCCAGTATGTCATTCCAGGTTTCTCCCACTCTGAAAGTTGCAATTGAGCCATCTGATCCTGCAGATATGGGTGCATTGATGAAGGGTTTACGGCTACTGAACAGGGCAGACCCTTTTGTAGAGGTCACGGTTTCTGCTAGAGGAGAACATGTTCTGTCTGCTGCAGGAGAAGTACATCTTGAAAGATGCATAAAAGATTTGAAGGATAGATTTGCAAGGGTAGGCTTGGAAGTGTCTAAGCCTCTTGTGTCTTTTAAAGAGACCATTTTGGGAGATGAATCCACACTCGAGAACTTAAAATCCTTTGTTGCCAGTTCAGAATATGTTGAGAAAGTGACACAAAATGGAAGGTGTGTTGTTCGGGTGAAGGTCCTGAAGCTCCCACCTTCTCTAACCAAGGTGATTGATGAAAGTTCTGATATTCTAGCAGGTATCCTTGGAGGCAAGGCTGCCCAGACAGGAGAGACCAATAAAAGTTTAAGCACACAGGTTGCAAGCACTGTAGAGGATGCGAATCCAATTGAAACACTAAGGAAACGTATGATGGAAGCTGTGGAAAGTGATATTTTGTCTAGTGGTGAGACTGACAAAGATCGGGCTGAGAAATGTAAAGTCCAATGGCAAAAACTTCTTAAAAGGATCTGGGCACTTGGCCCTTGGCATATTGGCCCTAACATCCTACTTGCTCCAGAAATGAAAGGAAAGGGTACTGACTCCTCTGTGCTCATCCGCGGCTCCTCTCATGTTTCTGAGAAATTAGGATTGGTGGATGCTTCTGACAATGACAATGACAATGACAATGATAATGCAGCTGCTGACACATCTTCAGAAGTAACTGAAGCATTGCATGCTGAAGCAGAGCGCCTTCAGAGCAGCGTTTTATCTGGGTTTCAACTGGCAACAGCAGCTGGACCTTTATGTGACGAGCCTATGTGGGGTTTAGCATTTGTGGTTGAAGCTTACATTTCACCATTAGCTGCTCAGTCTGATGAGTTAGAAACCTCCCACCAGCAACCTGAGCAATATGGTATCTTCACAGGACAGGTAATGACAGCCGTAAAGGATGCTTGTAGAGTAGCTGTGCTTCAAAAGAAACCACGGCTTGTGGAAGCTATGTACTTCTGTGAGTTGATCACGCCAACTGAACAATTGGGTAATATGTATGCTGTGCTTGGACGAAGGCGGACGAAAGTTTTAAAGGAGGAAATGCAGGAAGGCTCCGCATTGTTCACTGTACATGCCTATGTGCCGGTTTCTGAAAGCTTTGGTTTCGCGGATGAGCTTAGAAGGTGGACTGCTGGAGCTGCAAGTGCTCTTCTTGTGCTTAGCCACTGGGAAGCTCTTGATAAGGATCCTTTCTTTGTACCAAAAACGGATGAGGAAAAAGAGGAGTTTGGTGATGGTTCTAGTGTGCCTCCAAATACAGCAAGAAAACTGATAAATGCTGTAAGACGGCAGAAGGGTCTTCCAGTGGAGGAAAAAGTAGTGCAGCACGCGACAAAGCAGAGGACCTTGGCTCGTAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

1044

Amino Acids

115.06

Weight (kDa)

5.74

Isoelectric Point (pI)

41.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 19 - 233 7.2e-44 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 447 - 525 4e-10 Elongation factor Tu domain 2
EF-G_D2 PF22042 447 - 526 3.8e-08 Elongation factor G domain 2
EFG_III PF14492 543 - 605 1.5e-06 Elongation Factor G, domain III
EFL1 PF25118 661 - 762 2.1e-08 Elongation factor-like GTPase 1-like domain
EFG_C PF00679 901 - 986 5e-16 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AbsI CCTCGAGG 1 cut(s) 191
Acc36I ACCTGC 1 cut(s) 2021
AccB1I GGYRCC 1 cut(s) 590
AccI GTMKAC 1 cut(s) 348
AccII CGCG 6 cut(s) 207, 249, 335, 2334, 2876, 3098
AclI AACGTT 1 cut(s) 950
AclWI GGATC 8 cut(s) 334, 410, 1361, 1406, 1652, 2249, 2948, 2961
AcoI YGGCCR 4 cut(s) 3, 163, 585, 845
AcsI RAATTY 3 cut(s) 731, 1386, 1489
AcuI CTGAAG 7 cut(s) 942, 1233, 1997, 2412, 2453, 2460, 2475
AcyI GRCGYC 3 cut(s) 351, 363, 591
AdeI CACNNNGTG 1 cut(s) 420
AfaI GTAC 9 cut(s) 745, 890, 1158, 1277, 1776, 2311, 2720, 2838, 2967
AfiI CCNNNNNNNGG 8 cut(s) 178, 193, 318, 653, 1277, 1421, 1422, 2537
AflIII ACRYGT 1 cut(s) 1750
AjnI CCWGG 7 cut(s) 75, 317, 393, 398, 891, 1375, 1617
AjuI GAANNNNNNNTTGG 2 cut(s) 2348, 2380
AloI GAACNNNNNNTCC 4 cut(s) 1737, 1769, 1886, 1918
Alw21I GWGCWC 4 cut(s) 227, 636, 2328, 2917
Alw26I GTCTC 4 cut(s) 310, 1856, 2060, 2177
AlwI GGATC 8 cut(s) 334, 410, 1361, 1406, 1652, 2249, 2948, 2961
AlwNI CAGNNNCTG 2 cut(s) 2417, 2510
Ama87I CYCGRG 4 cut(s) 191, 277, 972, 1889
ApoI RAATTY 3 cut(s) 731, 1386, 1489
Asp700I GAANNNNTTC 1 cut(s) 1752
AspLEI GCGC 7 cut(s) 204, 209, 335, 393, 593, 1513, 2466
AsuC2I CCSGG 2 cut(s) 278, 279
AvaI CYCGRG 4 cut(s) 191, 277, 972, 1889
AvaII GGWCC 3 cut(s) 1972, 2513, 3111
BaeGI GKGCMC 1 cut(s) 2251
BamHI GGATCC 1 cut(s) 2953
BanI GGYRCC 1 cut(s) 590
BanII GRGCYC 3 cut(s) 227, 473, 636
BarI GAAGNNNNNNTAC 2 cut(s) 2670, 2702
BbsI GAAGAC 1 cut(s) 3059
Bbv12I GWGCWC 4 cut(s) 227, 636, 2328, 2917
BcgI CGANNNNNNTGC 4 cut(s) 51, 85, 548, 582
BciT130I CCWGG 7 cut(s) 77, 319, 395, 400, 893, 1377, 1619
BciVI GTATCC 1 cut(s) 2045
BclI TGATCA 2 cut(s) 429, 2733
BcnI CCSGG 2 cut(s) 278, 279
BcoDI GTCTC 4 cut(s) 310, 1856, 2060, 2177
BfaI CTAG 6 cut(s) 344, 1206, 1740, 2027, 2175, 3009
BfmI CTRYAG 5 cut(s) 295, 1357, 1662, 1764, 2103
BfoI RGCGCY 3 cut(s) 205, 594, 2467
BfuAI ACCTGC 1 cut(s) 2021
BfuI GTATCC 1 cut(s) 2045
BlpI GCTNAGC 2 cut(s) 323, 2927
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 893, 1377, 1619
Bme18I GGWCC 3 cut(s) 1972, 2513, 3111
BmeT110I CYCGRG 4 cut(s) 191, 277, 972, 1889
BmiI GGNNCC 7 cut(s) 130, 582, 592, 774, 2338, 2821, 2955
BmrFI CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 893, 1377, 1619
BmrI ACTGGG 1 cut(s) 2944
BmsI GCATC 8 cut(s) 150, 469, 556, 1138, 1778, 2101, 2362, 2656
BmuI ACTGGG 1 cut(s) 2944
BpiI GAAGAC 1 cut(s) 3059
BpmI CTGGAG 3 cut(s) 1588, 2274, 2922
Bpu10I CCTNAGC 1 cut(s) 2619
Bpu1102I GCTNAGC 2 cut(s) 323, 2927
BpuEI CTTGAG 1 cut(s) 890
BpuMI CCSGG 2 cut(s) 278, 279
Bsa29I ATCGAT 1 cut(s) 441
BsaBI GATNNNNATC 2 cut(s) 2115, 2952
BsaHI GRCGYC 3 cut(s) 351, 363, 591
BsaI GGTCTC 2 cut(s) 1856, 2060
Bsc4I CCNNNNNNNGG 8 cut(s) 178, 193, 318, 653, 1277, 1421, 1422, 2537
Bse118I RCCGGY 5 cut(s) 161, 583, 587, 874, 2851
Bse1I ACTGG 5 cut(s) 1605, 1914, 2502, 2939, 3071
Bse3DI GCAATG 3 cut(s) 664, 752, 2444
Bse8I GATNNNNATC 2 cut(s) 2115, 2952
BseBI CCWGG 7 cut(s) 77, 319, 395, 400, 893, 1377, 1619
BseCI ATCGAT 1 cut(s) 441
BseJI GATNNNNATC 2 cut(s) 2115, 2952
BseLI CCNNNNNNNGG 8 cut(s) 178, 193, 318, 653, 1277, 1421, 1422, 2537
BseMI GCAATG 3 cut(s) 664, 752, 2444
BseMII CTCAG 5 cut(s) 337, 2193, 2343, 2597, 2610
BseNI ACTGG 5 cut(s) 1605, 1914, 2502, 2939, 3071
BseSI GKGCMC 1 cut(s) 2251
BseX3I CGGCCG 3 cut(s) 163, 585, 845
BseYI CCCAGC 1 cut(s) 460
BsgI GTGCAG 1 cut(s) 3110
Bsh1236I CGCG 6 cut(s) 207, 249, 335, 2334, 2876, 3098
Bsh1285I CGRYCG 3 cut(s) 166, 588, 848
BshNI GGYRCC 1 cut(s) 590
BshVI ATCGAT 1 cut(s) 441
BsiEI CGRYCG 3 cut(s) 166, 588, 848
BsiHKAI GWGCWC 4 cut(s) 227, 636, 2328, 2917
BsiHKCI CYCGRG 4 cut(s) 191, 277, 972, 1889
BsiSI CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2852
BslFI GGGAC 1 cut(s) 825
BslI CCNNNNNNNGG 8 cut(s) 178, 193, 318, 653, 1277, 1421, 1422, 2537
BsmAI GTCTC 4 cut(s) 310, 1856, 2060, 2177
BsmFI GGGAC 1 cut(s) 825
BsmI GAATGC 1 cut(s) 1462
Bso31I GGTCTC 2 cut(s) 1856, 2060
BsoBI CYCGRG 4 cut(s) 191, 277, 972, 1889
Bsp1286I GDGCHC 6 cut(s) 227, 473, 636, 2251, 2328, 2917
Bsp1407I TGTACA 1 cut(s) 2836
Bsp1720I GCTNAGC 2 cut(s) 323, 2927
BspCNI CTCAG 5 cut(s) 336, 2194, 2344, 2596, 2611
BspDI ATCGAT 1 cut(s) 441
BspFNI CGCG 6 cut(s) 207, 249, 335, 2334, 2876, 3098
BspHI TCATGA 2 cut(s) 508, 1476
BspLI GGNNCC 7 cut(s) 130, 582, 592, 774, 2338, 2821, 2955
BspMAI CTGCAG 3 cut(s) 299, 1666, 1768
BspMI ACCTGC 1 cut(s) 2021
BspPI GGATC 8 cut(s) 334, 410, 1361, 1406, 1652, 2249, 2948, 2961
BspQI GCTCTTC 2 cut(s) 215, 2922
BspT107I GGYRCC 1 cut(s) 590
BspTNI GGTCTC 2 cut(s) 1856, 2060
BsrDI GCAATG 3 cut(s) 664, 752, 2444
BsrFI RCCGGY 5 cut(s) 161, 583, 587, 874, 2851
BsrGI TGTACA 1 cut(s) 2836
BsrI ACTGG 5 cut(s) 1605, 1914, 2502, 2939, 3071
BssAI RCCGGY 5 cut(s) 161, 583, 587, 874, 2851
BssNI GRCGYC 3 cut(s) 351, 363, 591
BssT1I CCWWGG 6 cut(s) 776, 1543, 1998, 2038, 2258, 3114
Bst2UI CCWGG 7 cut(s) 77, 319, 395, 400, 893, 1377, 1619
Bst4CI ACNGT 6 cut(s) 265, 1092, 1592, 1732, 2104, 2836
Bst6I CTCTTC 2 cut(s) 215, 2922
BstACI GRCGYC 3 cut(s) 351, 363, 591
BstAPI GCANNNNNTGC 1 cut(s) 2450
BstAUI TGTACA 1 cut(s) 2836
BstC8I GCNNGC 9 cut(s) 159, 163, 381, 585, 589, 1515, 2098, 2451, 3096
BstDSI CCRYGG 3 cut(s) 91, 2332, 2699
BstEII GGTNACC 1 cut(s) 986
BstENI CCTNNNNNAGG 1 cut(s) 191
BstFNI CGCG 6 cut(s) 207, 249, 335, 2334, 2876, 3098
BstH2I RGCGCY 3 cut(s) 205, 594, 2467
BstHHI GCGC 7 cut(s) 204, 209, 335, 393, 593, 1513, 2466
BstMAI GTCTC 4 cut(s) 310, 1856, 2060, 2177
BstMCI CGRYCG 3 cut(s) 166, 588, 848
BstMWI GCNNNNNNNGC 9 cut(s) 135, 1033, 1510, 2450, 2472, 2507, 2620, 2906, 2912
BstNI CCWGG 7 cut(s) 77, 319, 395, 400, 893, 1377, 1619
BstNSI RCATGY 4 cut(s) 1502, 1754, 2453, 2843
BstPI GGTNACC 1 cut(s) 986
BstSCI CCNGG 9 cut(s) 75, 276, 277, 317, 393, 398, 891, 1375, 1617
BstSFI CTRYAG 5 cut(s) 295, 1357, 1662, 1764, 2103
BstSLI GKGCMC 1 cut(s) 2251
BstUI CGCG 6 cut(s) 207, 249, 335, 2334, 2876, 3098
BstV2I GAAGAC 1 cut(s) 3059
BstX2I RGATCY 3 cut(s) 1353, 2241, 2953
BstYI RGATCY 3 cut(s) 1353, 2241, 2953
BstZI CGGCCG 3 cut(s) 163, 585, 845
Bsu15I ATCGAT 1 cut(s) 441
BsuI GTATCC 1 cut(s) 2045
BsuTUI ATCGAT 1 cut(s) 441
BtgI CCRYGG 3 cut(s) 91, 2332, 2699
BtgZI GCGATG 1 cut(s) 217
BtsIMutI CAGTG 4 cut(s) 2100, 2832, 2932, 3078
BveI ACCTGC 1 cut(s) 2021
Cac8I GCNNGC 9 cut(s) 159, 163, 381, 585, 589, 1515, 2098, 2451, 3096
CaiI CAGNNNCTG 2 cut(s) 2417, 2510
CciI TCATGA 2 cut(s) 508, 1476
CfoI GCGC 7 cut(s) 204, 209, 335, 393, 593, 1513, 2466
Cfr10I RCCGGY 5 cut(s) 161, 583, 587, 874, 2851
Cfr42I CCGCGG 1 cut(s) 2335
Cfr9I CCCGGG 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 441
CseI GACGC 3 cut(s) 61, 352, 359
Csp6I GTAC 9 cut(s) 744, 889, 1157, 1276, 1775, 2310, 2719, 2837, 2966
CviQI GTAC 9 cut(s) 744, 889, 1157, 1276, 1775, 2310, 2719, 2837, 2966
DinI GGCGCC 1 cut(s) 592
DraI TTTAAA 3 cut(s) 538, 1858, 2799
DraIII CACNNNGTG 1 cut(s) 420
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 4 cut(s) 3, 163, 585, 845
EagI CGGCCG 3 cut(s) 163, 585, 845
Eam1104I CTCTTC 2 cut(s) 215, 2922
EarI CTCTTC 2 cut(s) 215, 2922
EciI GGCGGA 3 cut(s) 230, 1184, 2800
Ecl136II GAGCTC 2 cut(s) 225, 634
EclXI CGGCCG 3 cut(s) 163, 585, 845
Eco130I CCWWGG 6 cut(s) 776, 1543, 1998, 2038, 2258, 3114
Eco147I AGGCCT 3 cut(s) 398, 1210, 1542
Eco24I GRGCYC 3 cut(s) 227, 473, 636
Eco31I GGTCTC 2 cut(s) 1856, 2060
Eco47I GGWCC 3 cut(s) 1972, 2513, 3111
Eco52I CGGCCG 3 cut(s) 163, 585, 845
Eco53kI GAGCTC 2 cut(s) 225, 634
Eco57I CTGAAG 7 cut(s) 942, 1233, 1997, 2412, 2453, 2460, 2475
Eco88I CYCGRG 4 cut(s) 191, 277, 972, 1889
Eco91I GGTNACC 1 cut(s) 986
EcoICRI GAGCTC 2 cut(s) 225, 634
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 3 cut(s) 773, 1972, 3111
EcoO65I GGTNACC 1 cut(s) 986
EcoRII CCWGG 7 cut(s) 75, 317, 393, 398, 891, 1375, 1617
EcoT14I CCWWGG 6 cut(s) 776, 1543, 1998, 2038, 2258, 3114
EcoT22I ATGCAT 2 cut(s) 754, 1793
EcoT38I GRGCYC 3 cut(s) 227, 473, 636
EgeI GGCGCC 1 cut(s) 592
EheI GGCGCC 1 cut(s) 592
ErhI CCWWGG 6 cut(s) 776, 1543, 1998, 2038, 2258, 3114
FalI AAGNNNNNCTT 4 cut(s) 1265, 1297, 1764, 1796
FaqI GGGAC 1 cut(s) 825
FauI CCCGC 1 cut(s) 820
FbaI TGATCA 2 cut(s) 429, 2733
FblI GTMKAC 1 cut(s) 348
FriOI GRGCYC 3 cut(s) 227, 473, 636
FseI GGCCGGCC 1 cut(s) 587
FspBI CTAG 6 cut(s) 344, 1206, 1740, 2027, 2175, 3009
GlaI GCGC 7 cut(s) 203, 208, 334, 392, 592, 1512, 2465
GsaI CCCAGC 1 cut(s) 464
GsuI CTGGAG 3 cut(s) 1588, 2274, 2922
HaeII RGCGCY 3 cut(s) 205, 594, 2467
HapII CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2852
HgaI GACGC 3 cut(s) 61, 352, 359
HhaI GCGC 7 cut(s) 204, 209, 335, 393, 593, 1513, 2466
Hin1I GRCGYC 3 cut(s) 351, 363, 591
Hin6I GCGC 7 cut(s) 202, 207, 333, 391, 591, 1511, 2464
HinP1I GCGC 7 cut(s) 202, 207, 333, 391, 591, 1511, 2464
HincII GTYRAC 2 cut(s) 88, 349
HindII GTYRAC 2 cut(s) 88, 349
HindIII AAGCTT 3 cut(s) 1194, 2559, 2863
HinfI GANTC 9 cut(s) 32, 182, 272, 1018, 1202, 1427, 1880, 2116, 2315
HpaII CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2852
Hpy166II GTNNAC 7 cut(s) 88, 349, 367, 889, 1691, 2832, 2895
Hpy8I GTNNAC 7 cut(s) 88, 349, 367, 889, 1691, 2832, 2895
Hpy99I CGWCG 4 cut(s) 332, 353, 359, 599
HpyCH4III ACNGT 6 cut(s) 265, 1092, 1592, 1732, 2104, 2836
HpyCH4IV ACGT 3 cut(s) 84, 950, 2140
HpyF10VI GCNNNNNNNGC 9 cut(s) 135, 1033, 1510, 2450, 2472, 2507, 2620, 2906, 2912
HpySE526I ACGT 3 cut(s) 84, 950, 2140
Hsp92I GRCGYC 3 cut(s) 351, 363, 591
HspAI GCGC 7 cut(s) 202, 207, 333, 391, 591, 1511, 2464
KasI GGCGCC 1 cut(s) 590
KroI GCCGGC 3 cut(s) 161, 583, 587
KroNI GCCGGC 3 cut(s) 163, 585, 589
Ksp22I TGATCA 2 cut(s) 429, 2733
KspI CCGCGG 1 cut(s) 2335
LguI GCTCTTC 2 cut(s) 215, 2922
LweI GCATC 8 cut(s) 150, 469, 556, 1138, 1778, 2101, 2362, 2656
MaeI CTAG 6 cut(s) 344, 1206, 1740, 2027, 2175, 3009
MaeII ACGT 3 cut(s) 84, 950, 2140
MaeIII GTNAC 8 cut(s) 173, 259, 986, 1110, 1726, 1936, 2434, 2522
MboII GAAGA 8 cut(s) 232, 811, 814, 1361, 2418, 2626, 2909, 3059
MfeI CAATTG 3 cut(s) 1644, 2121, 2750
MflI RGATCY 3 cut(s) 1353, 2241, 2953
MhlI GDGCHC 6 cut(s) 227, 473, 636, 2251, 2328, 2917
Mly113I GGCGCC 1 cut(s) 591
MlyI GAGTC 4 cut(s) 176, 266, 1027, 2309
MmeI TCCRAC 1 cut(s) 156
Mph1103I ATGCAT 2 cut(s) 754, 1793
MroNI GCCGGC 3 cut(s) 161, 583, 587
MroXI GAANNNNTTC 1 cut(s) 1752
MslI CAYNNNNRTG 2 cut(s) 215, 2844
MspA1I CMGCKG 4 cut(s) 138, 2334, 2414, 2510
MspI CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2852
MspR9I CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 893, 1377, 1619
MteI GCGCNGCGC 1 cut(s) 205
MunI CAATTG 3 cut(s) 1644, 2121, 2750
Mva1269I GAATGC 1 cut(s) 1462
MvaI CCWGG 7 cut(s) 77, 319, 395, 400, 893, 1377, 1619
MvnI CGCG 6 cut(s) 207, 249, 335, 2334, 2876, 3098
MwoI GCNNNNNNNGC 9 cut(s) 135, 1033, 1510, 2450, 2472, 2507, 2620, 2906, 2912
NaeI GCCGGC 3 cut(s) 163, 585, 589
NarI GGCGCC 1 cut(s) 591
NciI CCSGG 2 cut(s) 278, 279
NgoMIV GCCGGC 3 cut(s) 161, 583, 587
NlaIV GGNNCC 7 cut(s) 130, 582, 592, 774, 2338, 2821, 2955
NmeAIII GCCGAG 3 cut(s) 481, 739, 823
NmuCI GTSAC 5 cut(s) 259, 986, 1726, 1936, 2522
NsiI ATGCAT 2 cut(s) 754, 1793
NspI RCATGY 4 cut(s) 1502, 1754, 2453, 2843
PaeI GCATGC 1 cut(s) 2453
PaeR7I CTCGAG 2 cut(s) 191, 1889
PagI TCATGA 2 cut(s) 508, 1476
PceI AGGCCT 3 cut(s) 398, 1210, 1542
PciI ACATGT 1 cut(s) 1750
PciSI GCTCTTC 2 cut(s) 215, 2922
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 1462
PdiI GCCGGC 3 cut(s) 163, 585, 589
PdmI GAANNNNTTC 1 cut(s) 1752
PfeI GAWTC 5 cut(s) 32, 1202, 1427, 1880, 2116
PflFI GACNNNGTC 1 cut(s) 353
PleI GAGTC 4 cut(s) 176, 266, 1026, 2309
PluTI GGCGCC 1 cut(s) 594
PpsI GAGTC 4 cut(s) 176, 266, 1026, 2309
PpuMI RGGWCCY 2 cut(s) 1972, 3111
PscI ACATGT 1 cut(s) 1750
Psp124BI GAGCTC 2 cut(s) 227, 636
Psp1406I AACGTT 1 cut(s) 950
Psp5II RGGWCCY 2 cut(s) 1972, 3111
Psp6I CCWGG 7 cut(s) 75, 317, 393, 398, 891, 1375, 1617
PspEI GGTNACC 1 cut(s) 986
PspFI CCCAGC 1 cut(s) 460
PspGI CCWGG 7 cut(s) 75, 317, 393, 398, 891, 1375, 1617
PspN4I GGNNCC 7 cut(s) 130, 582, 592, 774, 2338, 2821, 2955
PspPPI RGGWCCY 2 cut(s) 1972, 3111
PspXI VCTCGAGB 1 cut(s) 191
PstI CTGCAG 3 cut(s) 299, 1666, 1768
PstNI CAGNNNCTG 2 cut(s) 2417, 2510
PsuI RGATCY 3 cut(s) 1353, 2241, 2953
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 2 cut(s) 2414, 2510
RigI GGCCGGCC 1 cut(s) 587
RsaI GTAC 9 cut(s) 745, 890, 1158, 1277, 1776, 2311, 2720, 2838, 2967
RsaNI GTAC 9 cut(s) 744, 889, 1157, 1276, 1775, 2310, 2719, 2837, 2966
RseI CAYNNNNRTG 2 cut(s) 215, 2844
SacI GAGCTC 2 cut(s) 227, 636
SacII CCGCGG 1 cut(s) 2335
SalI GTCGAC 1 cut(s) 347
SapI GCTCTTC 2 cut(s) 215, 2922
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 4 cut(s) 176, 266, 1027, 2309
ScrFI CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 893, 1377, 1619
SduI GDGCHC 6 cut(s) 227, 473, 636, 2251, 2328, 2917
SfaNI GCATC 8 cut(s) 150, 469, 556, 1138, 1778, 2101, 2362, 2656
SfcI CTRYAG 5 cut(s) 295, 1357, 1662, 1764, 2103
SfoI GGCGCC 1 cut(s) 592
Sfr274I CTCGAG 2 cut(s) 191, 1889
Sfr303I CCGCGG 1 cut(s) 2335
SgrBI CCGCGG 1 cut(s) 2335
SinI GGWCC 3 cut(s) 1972, 2513, 3111
SlaI CTCGAG 2 cut(s) 191, 1889
SmaI CCCGGG 1 cut(s) 279
SmiMI CAYNNNNRTG 2 cut(s) 215, 2844
SmlI CTYRAG 3 cut(s) 191, 869, 1889
SmoI CTYRAG 3 cut(s) 191, 869, 1889
SphI GCATGC 1 cut(s) 2453
SseBI AGGCCT 3 cut(s) 398, 1210, 1542
SspDI GGCGCC 1 cut(s) 590
SspMI CTAG 6 cut(s) 344, 1206, 1740, 2027, 2175, 3009
SstI GAGCTC 2 cut(s) 227, 636
StuI AGGCCT 3 cut(s) 398, 1210, 1542
StyD4I CCNGG 9 cut(s) 75, 276, 277, 317, 393, 398, 891, 1375, 1617
StyI CCWWGG 6 cut(s) 776, 1543, 1998, 2038, 2258, 3114
TaaI ACNGT 6 cut(s) 265, 1092, 1592, 1732, 2104, 2836
TaiI ACGT 3 cut(s) 87, 953, 2143
TaqI TCGA 7 cut(s) 192, 270, 348, 357, 405, 441, 1890
TatI WGTACW 5 cut(s) 743, 1156, 1774, 2718, 2836
TauI GCSGC 6 cut(s) 141, 168, 207, 317, 899, 2337
TfiI GAWTC 5 cut(s) 32, 1202, 1427, 1880, 2116
TscAI CASTG 4 cut(s) 2107, 2839, 2939, 3078
TseFI GTSAC 5 cut(s) 259, 986, 1726, 1936, 2522
Tsp45I GTSAC 5 cut(s) 259, 986, 1726, 1936, 2522
TspDTI ATGAA 8 cut(s) 233, 497, 1329, 1566, 1697, 1893, 2025, 2312
TspGWI ACGGA 3 cut(s) 161, 247, 2990
TspMI CCCGGG 1 cut(s) 277
TspRI CASTG 4 cut(s) 2107, 2839, 2939, 3078
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 3 cut(s) 1972, 2513, 3111
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 3 cut(s) 731, 1386, 1489
XceI RCATGY 4 cut(s) 1502, 1754, 2453, 2843
XhoI CTCGAG 2 cut(s) 191, 1889
XmaI CCCGGG 1 cut(s) 277
XmiI GTMKAC 1 cut(s) 348
XmnI GAANNNNTTC 1 cut(s) 1752
XspI CTAG 6 cut(s) 344, 1206, 1740, 2027, 2175, 3009
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 2 cut(s) 754, 1793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.