FvH4_4g04800

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002828
Physical Location & Seq
Forward (+)
4186331 .. 4187544
1214 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04800.t1

Sequence Viewer

Length: 1122 bp
ATGGCTCGGCCGATGTTTGTTGAGTTTGTGCTTGAGCCGGTTTGGAGACTGTACCAGGTGGCTTGGAAGGAGAGGGAGGAGGCTGAAGCGTTGGTTAGGAAAGTGATCAAAGCGTTTAGTTTGACTATACCATCCAGGGATTTGAAAGTCGATTTGAAGGGTATGGTTAAGGCTGTGATGAGTCATTGGCTTCCTGTGCATGAGGCTATCCTGTCGATGGTTATTACGTGTATGCCTGACCCGATTGCAGCACAGTTGTATAGGATTTCGCAGTTGCTTCCAAAAAGAGCTGAGGATGATTCTAGTGTACCTTCAAGGGCGGAGCTGGTGAGGAGATCGGTTGAAGCTTGTGATTCTAGGCCTGAAGCTCCTTGTGTTGCTTTTGTTTCGAAAATGTTCCCTGTCTCTACGAAAACACTTCCGAAGTATGGATTAGACGAGGAAGTTTTGGATAATACGTCTGATGAGGGTGAATTGGATGAGGAAATCAATGCAGAAGCATATTCAGGTGGCAAGTTGCATTCTTTGTATCTCATGATGGGCCAAAATTTACAACATGTACCAGAGGCGCAAGCAGGGGATATTGTGGCAATTCGAGGCCTCGGGCAGCATATACTTAAAACTGCAACACTTTCATCAACAAAGAATTGTTGGCCTTTCTCCGGTATGTCATTCCAGGTTTCTCCTACTCTGAAAGTTGCAATTGAGCCATCTTATTCTGCAGATATGGGTGCATTGAAGAAGGGTTTACGGCTGCTGAATAAGGCAGACCCTTTCGTAGAGGTCACGGAGTCTGGTAGAGGAGAACAATTTTTGTCTGCTGCAGGAGAAGTACATCTTGAAAGATGCATAAAAGATTTGAAAGATAGATTCGCAAGGGTAGGCTTTGTAGTGTCTAAGCCTCTTGTGTCTTTTAAAGAGACCATTTTGGGAGACGAATCCACACTTGAGAATTTAAAATCCTTTCTCGCCAGTACAGAATATGTTGAAAAAGCAACACAAGATGGAAGATGCGTTGTTCGGGTGAAGGTCCTGAAACTCCCACCTTCTCTAACTAAGGTGATTGATGAAAGTTCCAATATTCTAGCAGATATTCTAGCAGGCATGGCTGCCCAGAATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

374

Amino Acids

41.24

Weight (kDa)

5.83

Isoelectric Point (pI)

44.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFG_III PF14492 229 - 291 1.5e-06 Elongation Factor G, domain III
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 320
AcoI YGGCCR 1 cut(s) 8
AcsI RAATTY 2 cut(s) 547, 952
AcuI CTGAAG 2 cut(s) 105, 384
AfaI GTAC 5 cut(s) 53, 309, 561, 834, 976
AfiI CCNNNNNNNGG 3 cut(s) 217, 428, 662
AflIII ACRYGT 2 cut(s) 227, 556
AgsI TTSAA 8 cut(s) 145, 157, 315, 344, 739, 842, 862, 989
AjnI CCWGG 3 cut(s) 54, 134, 675
AluBI AGCT 4 cut(s) 290, 325, 347, 368
AluI AGCT 4 cut(s) 290, 325, 347, 368
Alw26I GTCTC 4 cut(s) 40, 409, 914, 927
Ama87I CYCGRG 1 cut(s) 602
AoxI GGCC 5 cut(s) 8, 359, 541, 598, 653
ApeKI GCWGC 5 cut(s) 248, 607, 754, 821, 1109
ApoI RAATTY 2 cut(s) 547, 952
Asp700I GAANNNNTTC 1 cut(s) 395
AspLEI GCGC 1 cut(s) 571
AspS9I GGNCC 2 cut(s) 541, 1030
AsuHPI GGTGA 4 cut(s) 340, 482, 1036, 1072
AsuII TTCGAA 1 cut(s) 389
AvaI CYCGRG 1 cut(s) 602
AvaII GGWCC 1 cut(s) 1030
BbvCI CCTCAGC 1 cut(s) 291
BbvI GCAGC 5 cut(s) 260, 619, 741, 808, 1096
BccI CCATC 5 cut(s) 139, 211, 532, 718, 998
BceAI ACGGC 1 cut(s) 767
BciT130I CCWGG 3 cut(s) 56, 136, 677
BclI TGATCA 1 cut(s) 105
BcoDI GTCTC 4 cut(s) 40, 409, 914, 927
BfaI CTAG 4 cut(s) 303, 357, 1085, 1097
BfmI CTRYAG 2 cut(s) 720, 822
BisI GCNGC 5 cut(s) 249, 608, 755, 822, 1110
BlsI GCNGC 5 cut(s) 250, 609, 756, 823, 1111
Bme1390I CCNGG 3 cut(s) 56, 136, 677
Bme18I GGWCC 1 cut(s) 1030
BmeT110I CYCGRG 1 cut(s) 602
BmgT120I GGNCC 2 cut(s) 541, 1030
BmrFI CCNGG 3 cut(s) 56, 136, 677
BmsI GCATC 2 cut(s) 836, 1001
Bpu10I CCTNAGC 1 cut(s) 291
Bpu14I TTCGAA 1 cut(s) 389
BpuEI CTTGAG 2 cut(s) 53, 968
BsaAI YACGTR 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 914
BsaJI CCNNGG 2 cut(s) 135, 601
BsaWI WCCGGW 1 cut(s) 662
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 428, 662
Bse118I RCCGGY 1 cut(s) 37
Bse1I ACTGG 1 cut(s) 972
BseBI CCWGG 3 cut(s) 56, 136, 677
BseDI CCNNGG 2 cut(s) 135, 601
BseGI GGATG 3 cut(s) 131, 301, 484
BseLI CCNNNNNNNGG 3 cut(s) 217, 428, 662
BseMII CTCAG 1 cut(s) 282
BseNI ACTGG 1 cut(s) 972
BseRI GAGGAG 3 cut(s) 92, 346, 816
BseX3I CGGCCG 1 cut(s) 8
BseXI GCAGC 5 cut(s) 260, 619, 741, 808, 1096
Bsh1285I CGRYCG 1 cut(s) 11
BshFI GGCC 5 cut(s) 10, 361, 543, 600, 655
BsiEI CGRYCG 1 cut(s) 11
BsiHKCI CYCGRG 1 cut(s) 602
BsiSI CCGG 2 cut(s) 38, 663
BslI CCNNNNNNNGG 3 cut(s) 217, 428, 662
BsmAI GTCTC 4 cut(s) 40, 409, 914, 927
BsmBI CGTCTC 1 cut(s) 927
BsmI GAATGC 1 cut(s) 520
BsnI GGCC 5 cut(s) 10, 361, 543, 600, 655
Bso31I GGTCTC 1 cut(s) 914
BsoBI CYCGRG 1 cut(s) 602
Bsp119I TTCGAA 1 cut(s) 389
Bsp143I GATC 2 cut(s) 105, 335
BspACI CCGC 1 cut(s) 320
BspANI GGCC 5 cut(s) 10, 361, 543, 600, 655
BspCNI CTCAG 1 cut(s) 283
BspHI TCATGA 1 cut(s) 534
BspMAI CTGCAG 2 cut(s) 724, 826
BspT104I TTCGAA 1 cut(s) 389
BspTNI GGTCTC 1 cut(s) 914
BsrFI RCCGGY 1 cut(s) 37
BsrI ACTGG 1 cut(s) 972
BssAI RCCGGY 1 cut(s) 37
BssECI CCNNGG 2 cut(s) 135, 601
BssMI GATC 2 cut(s) 105, 335
Bst2UI CCWGG 3 cut(s) 56, 136, 677
Bst4CI ACNGT 2 cut(s) 51, 255
BstBAI YACGTR 1 cut(s) 228
BstBI TTCGAA 1 cut(s) 389
BstC8I GCNNGC 2 cut(s) 573, 1102
BstDEI CTNAG 3 cut(s) 291, 897, 1056
BstF5I GGATG 3 cut(s) 131, 301, 484
BstHHI GCGC 1 cut(s) 571
BstKTI GATC 2 cut(s) 108, 338
BstMAI GTCTC 4 cut(s) 40, 409, 914, 927
BstMBI GATC 2 cut(s) 105, 335
BstMCI CGRYCG 1 cut(s) 11
BstMWI GCNNNNNNNGC 2 cut(s) 196, 1106
BstNI CCWGG 3 cut(s) 56, 136, 677
BstNSI RCATGY 1 cut(s) 560
BstSCI CCNGG 3 cut(s) 54, 134, 675
BstSFI CTRYAG 2 cut(s) 720, 822
BstV1I GCAGC 5 cut(s) 260, 619, 741, 808, 1096
BstZI CGGCCG 1 cut(s) 8
BsuRI GGCC 5 cut(s) 10, 361, 543, 600, 655
BtsCI GGATG 3 cut(s) 131, 301, 484
Cac8I GCNNGC 2 cut(s) 573, 1102
CciI TCATGA 1 cut(s) 534
CfoI GCGC 1 cut(s) 571
Cfr10I RCCGGY 1 cut(s) 37
Cfr13I GGNCC 2 cut(s) 541, 1030
CsiI ACCWGGT 1 cut(s) 54
Csp6I GTAC 5 cut(s) 52, 308, 560, 833, 975
CviAII CATG 4 cut(s) 200, 535, 557, 1105
CviQI GTAC 5 cut(s) 52, 308, 560, 833, 975
DdeI CTNAG 3 cut(s) 291, 897, 1056
DpnI GATC 2 cut(s) 107, 337
DpnII GATC 2 cut(s) 105, 335
DraI TTTAAA 2 cut(s) 916, 957
EaeI YGGCCR 1 cut(s) 8
EagI CGGCCG 1 cut(s) 8
EciI GGCGGA 1 cut(s) 335
EclXI CGGCCG 1 cut(s) 8
Eco147I AGGCCT 2 cut(s) 361, 600
Eco31I GGTCTC 1 cut(s) 914
Eco47I GGWCC 1 cut(s) 1030
Eco52I CGGCCG 1 cut(s) 8
Eco57I CTGAAG 2 cut(s) 105, 384
Eco88I CYCGRG 1 cut(s) 602
EcoO109I RGGNCCY 1 cut(s) 1030
EcoRII CCWGG 3 cut(s) 54, 134, 675
EcoT22I ATGCAT 1 cut(s) 851
Esp3I CGTCTC 1 cut(s) 927
FaeI CATG 4 cut(s) 203, 538, 560, 1108
FalI AAGNNNNNCTT 2 cut(s) 822, 854
FatI CATG 4 cut(s) 199, 534, 556, 1104
FbaI TGATCA 1 cut(s) 105
Fnu4HI GCNGC 5 cut(s) 249, 608, 755, 822, 1110
FokI GGATG 3 cut(s) 118, 308, 491
Fsp4HI GCNGC 5 cut(s) 249, 608, 755, 822, 1110
FspBI CTAG 4 cut(s) 303, 357, 1085, 1097
GlaI GCGC 1 cut(s) 570
GluI GCNGC 5 cut(s) 249, 608, 755, 822, 1110
HaeIII GGCC 5 cut(s) 10, 361, 543, 600, 655
HapII CCGG 2 cut(s) 38, 663
HhaI GCGC 1 cut(s) 571
Hin1II CATG 4 cut(s) 203, 538, 560, 1108
Hin6I GCGC 1 cut(s) 569
HinP1I GCGC 1 cut(s) 569
HindIII AAGCTT 1 cut(s) 345
HinfI GANTC 6 cut(s) 181, 299, 353, 791, 870, 938
HpaII CCGG 2 cut(s) 38, 663
HphI GGTGA 4 cut(s) 340, 482, 1036, 1072
Hpy166II GTNNAC 2 cut(s) 308, 749
Hpy188I TCNGA 3 cut(s) 423, 463, 693
Hpy188III TCNNGA 3 cut(s) 535, 839, 1033
Hpy8I GTNNAC 2 cut(s) 308, 749
HpyAV CCTTC 6 cut(s) 61, 151, 321, 736, 1021, 1056
HpyCH4III ACNGT 2 cut(s) 51, 255
HpyCH4IV ACGT 2 cut(s) 227, 458
HpyF10VI GCNNNNNNNGC 2 cut(s) 196, 1106
HpyF3I CTNAG 3 cut(s) 291, 897, 1056
HpySE526I ACGT 2 cut(s) 227, 458
Hsp92II CATG 4 cut(s) 203, 538, 560, 1108
HspAI GCGC 1 cut(s) 569
Ksp22I TGATCA 1 cut(s) 105
Kzo9I GATC 2 cut(s) 105, 335
LmnI GCTCC 2 cut(s) 322, 373
Lsp1109I GCAGC 5 cut(s) 260, 619, 741, 808, 1096
LweI GCATC 2 cut(s) 836, 1001
MabI ACCWGGT 1 cut(s) 54
MaeI CTAG 4 cut(s) 303, 357, 1085, 1097
MaeII ACGT 2 cut(s) 227, 458
MaeIII GTNAC 1 cut(s) 784
MalI GATC 2 cut(s) 107, 337
MboI GATC 2 cut(s) 105, 335
MboII GAAGA 2 cut(s) 751, 1020
MfeI CAATTG 1 cut(s) 702
MluCI AATT 8 cut(s) 473, 547, 591, 646, 702, 809, 952, 1117
MlyI GAGTC 2 cut(s) 190, 800
Mph1103I ATGCAT 1 cut(s) 851
MroXI GAANNNNTTC 1 cut(s) 395
MseI TTAA 5 cut(s) 168, 618, 915, 956, 1120
MspI CCGG 2 cut(s) 38, 663
MspR9I CCNGG 3 cut(s) 56, 136, 677
MunI CAATTG 1 cut(s) 702
Mva1269I GAATGC 1 cut(s) 520
MvaI CCWGG 3 cut(s) 56, 136, 677
MwoI GCNNNNNNNGC 2 cut(s) 196, 1106
NdeII GATC 2 cut(s) 105, 335
NlaIII CATG 4 cut(s) 203, 538, 560, 1108
NmuCI GTSAC 1 cut(s) 784
NsiI ATGCAT 1 cut(s) 851
NspI RCATGY 1 cut(s) 560
NspV TTCGAA 1 cut(s) 389
PagI TCATGA 1 cut(s) 534
PceI AGGCCT 2 cut(s) 361, 600
PciI ACATGT 1 cut(s) 556
PctI GAATGC 1 cut(s) 520
PdmI GAANNNNTTC 1 cut(s) 395
PfeI GAWTC 4 cut(s) 299, 353, 870, 938
PkrI GCNGC 5 cut(s) 250, 609, 756, 823, 1111
PleI GAGTC 2 cut(s) 189, 799
PpsI GAGTC 2 cut(s) 189, 799
Ppu21I YACGTR 1 cut(s) 228
PpuMI RGGWCCY 1 cut(s) 1030
PscI ACATGT 1 cut(s) 556
Psp5II RGGWCCY 1 cut(s) 1030
Psp6I CCWGG 3 cut(s) 54, 134, 675
PspGI CCWGG 3 cut(s) 54, 134, 675
PspPI GGNCC 2 cut(s) 541, 1030
PspPPI RGGWCCY 1 cut(s) 1030
PstI CTGCAG 2 cut(s) 724, 826
RsaI GTAC 5 cut(s) 53, 309, 561, 834, 976
RsaNI GTAC 5 cut(s) 52, 308, 560, 833, 975
SaqAI TTAA 5 cut(s) 168, 618, 915, 956, 1120
SatI GCNGC 5 cut(s) 249, 608, 755, 822, 1110
Sau3AI GATC 2 cut(s) 105, 335
Sau96I GGNCC 2 cut(s) 541, 1030
SchI GAGTC 2 cut(s) 190, 800
ScrFI CCNGG 3 cut(s) 56, 136, 677
SexAI ACCWGGT 1 cut(s) 54
SfaNI GCATC 2 cut(s) 836, 1001
SfcI CTRYAG 2 cut(s) 720, 822
SfuI TTCGAA 1 cut(s) 389
SinI GGWCC 1 cut(s) 1030
SmlI CTYRAG 2 cut(s) 32, 947
SmoI CTYRAG 2 cut(s) 32, 947
Sse9I AATT 8 cut(s) 473, 547, 591, 646, 702, 809, 952, 1117
SseBI AGGCCT 2 cut(s) 361, 600
SsiI CCGC 1 cut(s) 320
SspI AATATT 1 cut(s) 1081
SspMI CTAG 4 cut(s) 303, 357, 1085, 1097
StuI AGGCCT 2 cut(s) 361, 600
StyD4I CCNGG 3 cut(s) 54, 134, 675
TaaI ACNGT 2 cut(s) 51, 255
TaiI ACGT 2 cut(s) 230, 461
TaqI TCGA 4 cut(s) 150, 215, 389, 595
TasI AATT 8 cut(s) 473, 547, 591, 646, 702, 809, 952, 1117
TatI WGTACW 2 cut(s) 832, 974
TfiI GAWTC 4 cut(s) 299, 353, 870, 938
Tru1I TTAA 5 cut(s) 168, 618, 915, 956, 1120
Tru9I TTAA 5 cut(s) 168, 618, 915, 956, 1120
TseFI GTSAC 1 cut(s) 784
TseI GCWGC 5 cut(s) 248, 607, 754, 821, 1109
Tsp45I GTSAC 1 cut(s) 784
TspDTI ATGAA 2 cut(s) 624, 1083
TspGWI ACGGA 1 cut(s) 803
VpaK11BI GGWCC 1 cut(s) 1030
XapI RAATTY 2 cut(s) 547, 952
XceI RCATGY 1 cut(s) 560
XmnI GAANNNNTTC 1 cut(s) 395
XspI CTAG 4 cut(s) 303, 357, 1085, 1097
Zsp2I ATGCAT 1 cut(s) 851
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.