Rw6G017390

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr6
OG0002828
Physical Location & Seq
Reverse (-)
34491163 .. 34496535
5373 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw6G017390.1

Sequence Viewer

Length: 3114 bp
ATGGCCGACCCGTCCCAACAACCCCAACCTGAATCAACGACACCCGACACCCGAAAGCTCCGAAACATCTGCGTCCTGGCCCACGTCGACCACGGCAAGACGACCCTCTGCGACCACCTCATCTCCGGCTCCGACAGCGGCATCATCCACCCCAAGCTCGCCGGGCGCCTCCGCTACATGGACTCCCTCCTCGAGGAGCAGCGCCGCGCCATCACCATGAAGAGCTCCTCCATCGCCCTCCGCCACTGCGACCACTCCGTCACCGTCATCGACTCCCCGGGCCACATGGACTTCTGCAGCGAAGTCTCCACCGCCGCCAGGCTCAGCGACGGCGCGTTGATCCTAGTCGACGCCGTCGAGGGCGTCCACATCCAAACGCACGCCGTCTTGCGCCAGGCCTGGATCGAGAAGCTCACGCCGTGCTTGGTGATCAACAAGATCGATAGGTTGATCGCGGAGCTGGGAATGAGCCCTATGGATGCGTATGTAAAGTTGTTGAAGATTGTTCATGAGGTTAATAACATTGTTAGTGCTTTTAAATCTGTGAAATACTTGAACGATGTGGATGCCATTCTTTCGGGGCCGGCCGGCGCCGACGAGTTGGCGTTTGAGGAGATTGAGGATGATGAGGAGCTCATGTTTCAGCCCCAGAAGGGCAATGTGGCCTTTGTGTGTGCTTTGGATGGTTGGGGGTTTAGTATTAGGCAATTTGCCGAGTTTTATGAGGCGAAATTCGGGTGGAGTACTAATGCATTGCAGAAGGCTTTATGGGGGCCTTGGTATTTTAATCACAAGGAGAAGAAGATTGTGGGACAGAAGGGTGTGGCGGGTTTTAAAAAGGCTCGGCCGATGTTTGTTGAGTTTGTGCTTGAGCCGGTTTGGAGTGTGTACCAGGCGGCTTTGAAGGAGAAGGAGGAGGCTCAAGTGATGGTTCAGAAGGTGATTAGAACGTTTAATTTGACTATACCACCTCGGGATTTGAAGGGTGACCCAAAAGGTATGGTTCAGGCTGTGATGAGTCGGTGGCTTCCTCTGCATGAGGCTATCTTGAACATGGTTATTAGGTGTATGCCTGACCCGATTGCAGCACAGTCATTTCGGATTACAAGGTTACTTCCAAAGAGAGAGGTTGTGGGAGATGGGGTTGATGCTAATGTACTTGCAGAGGCGGAGCTTGTGAGAAGTTCGGTGGAAGCTTGTGATTCTAGGCCTGAAGCTCCTTGTGTTGTTTTTGTTTCGAAAATGTTTGCTGTGCCAACCAAAACACTTCCAAAGTATGGCTTGGATGGGGAGATTTTGGATAATACATCTGATGAAGGTGAATTGGATGAGTGCTTCCTTGCGTTTGCAAGGATCTATAGTGGGGTTCTTCGTCCTGGTGAGAAAATTTATGTGCTTTCAGCTTTATATGATCCCTTAAAAGGGGAATCAATGCAGAAGCATATTCAGGTGGCTAAGTTGCATTCTCTGTATCTCATGATGGGCCAAAATTTACAGCATGTGCCAGAGGCGCAGGCAGGGGATGTTGTGGCAATCCGAGGCCTTGGGCAGCATATACTGAAAACTGCAACACTTTCATCAACAGAGAACTGTTGGCCTTTCTCCAGTATGTCATTCCAGGTTTCTCCCACTCTGAAAGTTGCAATTGAGCCATCTGATCCTGCAGATATGGGTGCATTGATGAAGGGTTTACGGCTGCTGAACAGGGCAGACCCTTTTGTAGAGGTCACGGTTTCTGCTAGAGGAGAACATGTTCTGTCTGCTGCAGGAGAAGTACATCTTGAAAGATGCATAAAAGATTTGAAGGATAGATTTGCAAGGGTAGGCTTGGAAGTGTCTAAGCCTCTTGTGTCTTTTAAAGAGACCATTTTGGGAGATGAATCCACACTTGAGAACTTAAAATCCTTTGTTGCCAGTTCAGAATATGTTGAGAAAGTGACACAAAATGGAAGGTGTGTTGTTCGGGTGAAGGTCCTGAAGCTCCCACCTTCTCTAACCAAGGTGATTGATGAAAGTTCTGATATTCTAGCAGATATCCTTGGAGGCAAGGCTGCCCAGACAGGAGAGACCAATAAAAGTTTAAACACACAGGTTGCAAGCACTGTAGAGGATGCGAATCCAATTGAAACACTAAGGAAACGTATGATGGAAGCTGTGGAAAGTGATATTTTGTCTAGTGGTGAGACTGACAAAGTTCGGGCTGAGAAATGTAAAGTCCAATGGCAAAAACTTCTTAAAAGGATCTGGGCACTTGGCCCTTGGCATATTGGCCCTAACATCCTACTTGCTCCAGAAATGAAAGGAAAGGGTACTGACTCCTCTGTGCTCATCCGCGGCTCCTCTCATGTTTCTGAGAAATTAGGATTGGTGGATGCTTCTGAAAATGATAATGCAGCTGCTGACACATCTTCAGAAGTAACTGAAGCATTGTATGCTGAAGCAGAGCGCCTTCATAGCAGCGTTTTATCTGGGTTTCAACTGGCAACAGCAGCTGGACCTTTATGTGACGAGCCTATGTGGGGTTTAGCATTTGTGGTTGAAGCTTACATTTCACCATTAGCTGCTCAGTCTGATGAGTTAGAAACCTCCCACCAGCAACCTGAGCAATATGGTATCTTCACAGGACAGGTAATGACAGCCATTAAGGATGCTTGTAGGGTAGCTGTGCTTCAAAAGAAACCACGGCTTGTGGAAGCTATGTACTTCTGTGAGTTGATCACGCCAACTGAACAATTGGGTAATATGTATGCTGTGCTTGGACGAAGGCGGACGAAAGTTTTAAAGGAGGAAATGCAGGAAGGCTCTGCATTGTTCACTGTACATGCCTATGTGCCGGTTTCTGAAAGCTTTGGTTTTGCGGATGAGCTTAGAAGGTGGACTGCTGGAGCTGCAAGTGCTCTTCTTGTGCTTAGCCACTGGGAAGCTCTTGATAAGGATCCTTTCTTTGTACCAAAAACGGATGAGGAAAAAGAGGAGTTTGGTGATGGTTCTAGTGTGCCTCCAAATACAGCAAGAAAACTGATAAATGCTGTAAGACGGCAGAAGGGTCTTCCAGTGGAGGAAAAAGTAGTGCAGCACGCGACAAAGCAGAGGACCTTGGCTCGTAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

1037

Amino Acids

114.42

Weight (kDa)

5.82

Isoelectric Point (pI)

42.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 19 - 233 2.9e-43 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 447 - 525 9.2e-10 Elongation factor Tu domain 2
EF-G_D2 PF22042 447 - 525 9.7e-08 Elongation factor G domain 2
EFG_III PF14492 543 - 605 1.5e-06 Elongation Factor G, domain III
EFL1 PF25118 661 - 762 6.2e-09 Elongation factor-like GTPase 1-like domain
EFG_C PF00679 895 - 980 4.9e-16 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AbsI CCTCGAGG 1 cut(s) 191
AccB1I GGYRCC 2 cut(s) 165, 590
AccB7I CCANNNNNTGG 1 cut(s) 1277
AccI GTMKAC 2 cut(s) 87, 348
AccII CGCG 5 cut(s) 207, 335, 455, 2334, 3080
AclI AACGTT 1 cut(s) 950
AclWI GGATC 8 cut(s) 334, 410, 1361, 1406, 1652, 2249, 2930, 2943
AcoI YGGCCR 3 cut(s) 3, 585, 845
AcsI RAATTY 3 cut(s) 731, 1386, 1489
AcuI CTGAAG 5 cut(s) 1233, 1997, 2394, 2442, 2457
AcyI GRCGYC 4 cut(s) 166, 351, 363, 591
AdeI CACNNNGTG 1 cut(s) 420
AfaI GTAC 8 cut(s) 745, 890, 1158, 1776, 2311, 2702, 2820, 2949
AfiI CCNNNNNNNGG 8 cut(s) 178, 193, 318, 653, 1277, 1421, 1422, 2519
AflIII ACRYGT 1 cut(s) 1750
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 7 cut(s) 75, 317, 393, 398, 891, 1375, 1617
AjuI GAANNNNNNNTTGG 2 cut(s) 2348, 2380
AloI GAACNNNNNNTCC 4 cut(s) 1737, 1769, 1886, 1918
Alw21I GWGCWC 4 cut(s) 227, 636, 2328, 2899
Alw26I GTCTC 4 cut(s) 310, 1856, 2060, 2177
AlwI GGATC 8 cut(s) 334, 410, 1361, 1406, 1652, 2249, 2930, 2943
AlwNI CAGNNNCTG 2 cut(s) 2399, 2492
Ama87I CYCGRG 3 cut(s) 191, 277, 972
ApoI RAATTY 3 cut(s) 731, 1386, 1489
Asp700I GAANNNNTTC 1 cut(s) 1752
AspLEI GCGC 8 cut(s) 168, 204, 209, 335, 393, 593, 1513, 2448
AsuC2I CCSGG 3 cut(s) 163, 278, 279
AsuII TTCGAA 1 cut(s) 1238
AvaI CYCGRG 3 cut(s) 191, 277, 972
AvaII GGWCC 3 cut(s) 1972, 2495, 3093
BaeGI GKGCMC 1 cut(s) 2251
BamHI GGATCC 1 cut(s) 2935
BanI GGYRCC 2 cut(s) 165, 590
BanII GRGCYC 3 cut(s) 227, 473, 636
BarI GAAGNNNNNNTAC 2 cut(s) 2652, 2684
BbsI GAAGAC 1 cut(s) 3041
Bbv12I GWGCWC 4 cut(s) 227, 636, 2328, 2899
BceAI ACGGC 8 cut(s) 109, 338, 346, 368, 403, 1709, 2699, 3053
BcgI CGANNNNNNTGC 4 cut(s) 51, 85, 548, 582
BciT130I CCWGG 7 cut(s) 77, 319, 395, 400, 893, 1377, 1619
BclI TGATCA 2 cut(s) 429, 2715
BcnI CCSGG 3 cut(s) 163, 278, 279
BcoDI GTCTC 4 cut(s) 310, 1856, 2060, 2177
BfaI CTAG 6 cut(s) 344, 1206, 1740, 2027, 2175, 2991
BfmI CTRYAG 5 cut(s) 295, 1357, 1662, 1764, 2103
BfoI RGCGCY 4 cut(s) 169, 205, 594, 2449
BlpI GCTNAGC 2 cut(s) 323, 2909
BmcAI AGTACT 1 cut(s) 745
Bme18I GGWCC 3 cut(s) 1972, 2495, 3093
BmeT110I CYCGRG 3 cut(s) 191, 277, 972
BmgBI CACGTC 1 cut(s) 85
BmiI GGNNCC 7 cut(s) 130, 167, 582, 592, 774, 2338, 2937
BmrI ACTGGG 1 cut(s) 2926
BmsI GCATC 8 cut(s) 150, 469, 556, 1138, 1778, 2101, 2362, 2638
BmuI ACTGGG 1 cut(s) 2926
BpiI GAAGAC 1 cut(s) 3041
BpmI CTGGAG 3 cut(s) 1588, 2274, 2904
Bpu10I CCTNAGC 1 cut(s) 2601
Bpu1102I GCTNAGC 2 cut(s) 323, 2909
Bpu14I TTCGAA 1 cut(s) 1238
BpuEI CTTGAG 3 cut(s) 890, 906, 1910
BpuMI CCSGG 3 cut(s) 163, 278, 279
Bsa29I ATCGAT 1 cut(s) 441
BsaBI GATNNNNATC 2 cut(s) 2115, 2934
BsaHI GRCGYC 4 cut(s) 166, 351, 363, 591
BsaI GGTCTC 2 cut(s) 1856, 2060
Bsc4I CCNNNNNNNGG 8 cut(s) 178, 193, 318, 653, 1277, 1421, 1422, 2519
Bse118I RCCGGY 4 cut(s) 583, 587, 874, 2833
Bse1I ACTGG 5 cut(s) 1605, 1914, 2484, 2921, 3053
Bse3DI GCAATG 2 cut(s) 664, 752
Bse8I GATNNNNATC 2 cut(s) 2115, 2934
BseBI CCWGG 7 cut(s) 77, 319, 395, 400, 893, 1377, 1619
BseCI ATCGAT 1 cut(s) 441
BseJI GATNNNNATC 2 cut(s) 2115, 2934
BseLI CCNNNNNNNGG 8 cut(s) 178, 193, 318, 653, 1277, 1421, 1422, 2519
BseMI GCAATG 2 cut(s) 664, 752
BseMII CTCAG 5 cut(s) 337, 2193, 2343, 2579, 2592
BseNI ACTGG 5 cut(s) 1605, 1914, 2484, 2921, 3053
BseSI GKGCMC 1 cut(s) 2251
BseX3I CGGCCG 2 cut(s) 585, 845
BseYI CCCAGC 1 cut(s) 460
BsgI GTGCAG 1 cut(s) 3092
Bsh1236I CGCG 5 cut(s) 207, 335, 455, 2334, 3080
Bsh1285I CGRYCG 2 cut(s) 588, 848
BshNI GGYRCC 2 cut(s) 165, 590
BshVI ATCGAT 1 cut(s) 441
BsiEI CGRYCG 2 cut(s) 588, 848
BsiHKAI GWGCWC 4 cut(s) 227, 636, 2328, 2899
BsiHKCI CYCGRG 3 cut(s) 191, 277, 972
BsiSI CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2834
BslFI GGGAC 1 cut(s) 825
BslI CCNNNNNNNGG 8 cut(s) 178, 193, 318, 653, 1277, 1421, 1422, 2519
BsmAI GTCTC 4 cut(s) 310, 1856, 2060, 2177
BsmFI GGGAC 1 cut(s) 825
BsmI GAATGC 1 cut(s) 1462
Bso31I GGTCTC 2 cut(s) 1856, 2060
BsoBI CYCGRG 3 cut(s) 191, 277, 972
Bsp119I TTCGAA 1 cut(s) 1238
Bsp1286I GDGCHC 6 cut(s) 227, 473, 636, 2251, 2328, 2899
Bsp1407I TGTACA 1 cut(s) 2818
Bsp1720I GCTNAGC 2 cut(s) 323, 2909
BspCNI CTCAG 5 cut(s) 336, 2194, 2344, 2578, 2593
BspDI ATCGAT 1 cut(s) 441
BspFNI CGCG 5 cut(s) 207, 335, 455, 2334, 3080
BspHI TCATGA 2 cut(s) 508, 1476
BspLI GGNNCC 7 cut(s) 130, 167, 582, 592, 774, 2338, 2937
BspMAI CTGCAG 3 cut(s) 299, 1666, 1768
BspPI GGATC 8 cut(s) 334, 410, 1361, 1406, 1652, 2249, 2930, 2943
BspQI GCTCTTC 2 cut(s) 215, 2904
BspT104I TTCGAA 1 cut(s) 1238
BspT107I GGYRCC 2 cut(s) 165, 590
BspTNI GGTCTC 2 cut(s) 1856, 2060
BsrDI GCAATG 2 cut(s) 664, 752
BsrFI RCCGGY 4 cut(s) 583, 587, 874, 2833
BsrGI TGTACA 1 cut(s) 2818
BsrI ACTGG 5 cut(s) 1605, 1914, 2484, 2921, 3053
BssAI RCCGGY 4 cut(s) 583, 587, 874, 2833
BssNI GRCGYC 4 cut(s) 166, 351, 363, 591
BssT1I CCWWGG 6 cut(s) 776, 1543, 1998, 2038, 2258, 3096
Bst2UI CCWGG 7 cut(s) 77, 319, 395, 400, 893, 1377, 1619
Bst4CI ACNGT 6 cut(s) 265, 1092, 1592, 1732, 2104, 2818
Bst6I CTCTTC 2 cut(s) 215, 2904
BstACI GRCGYC 4 cut(s) 166, 351, 363, 591
BstAPI GCANNNNNTGC 1 cut(s) 2432
BstAUI TGTACA 1 cut(s) 2818
BstBI TTCGAA 1 cut(s) 1238
BstC8I GCNNGC 7 cut(s) 159, 381, 585, 589, 1515, 2098, 3078
BstDSI CCRYGG 3 cut(s) 91, 2332, 2681
BstEII GGTNACC 1 cut(s) 986
BstENI CCTNNNNNAGG 1 cut(s) 191
BstFNI CGCG 5 cut(s) 207, 335, 455, 2334, 3080
BstH2I RGCGCY 4 cut(s) 169, 205, 594, 2449
BstHHI GCGC 8 cut(s) 168, 204, 209, 335, 393, 593, 1513, 2448
BstMAI GTCTC 4 cut(s) 310, 1856, 2060, 2177
BstMCI CGRYCG 2 cut(s) 588, 848
BstNI CCWGG 7 cut(s) 77, 319, 395, 400, 893, 1377, 1619
BstNSI RCATGY 3 cut(s) 1502, 1754, 2825
BstPI GGTNACC 1 cut(s) 986
BstSFI CTRYAG 5 cut(s) 295, 1357, 1662, 1764, 2103
BstSLI GKGCMC 1 cut(s) 2251
BstUI CGCG 5 cut(s) 207, 335, 455, 2334, 3080
BstV2I GAAGAC 1 cut(s) 3041
BstX2I RGATCY 3 cut(s) 1353, 2241, 2935
BstYI RGATCY 3 cut(s) 1353, 2241, 2935
BstZI CGGCCG 2 cut(s) 585, 845
Bsu15I ATCGAT 1 cut(s) 441
BsuTUI ATCGAT 1 cut(s) 441
BtgI CCRYGG 3 cut(s) 91, 2332, 2681
BtgZI GCGATG 1 cut(s) 217
BtrI CACGTC 1 cut(s) 85
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 5 cut(s) 244, 2100, 2814, 2914, 3060
Cac8I GCNNGC 7 cut(s) 159, 381, 585, 589, 1515, 2098, 3078
CaiI CAGNNNCTG 2 cut(s) 2399, 2492
CciI TCATGA 2 cut(s) 508, 1476
CfoI GCGC 8 cut(s) 168, 204, 209, 335, 393, 593, 1513, 2448
Cfr10I RCCGGY 4 cut(s) 583, 587, 874, 2833
Cfr42I CCGCGG 1 cut(s) 2335
Cfr9I CCCGGG 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 441
CseI GACGC 3 cut(s) 61, 352, 359
Csp6I GTAC 8 cut(s) 744, 889, 1157, 1775, 2310, 2701, 2819, 2948
CviQI GTAC 8 cut(s) 744, 889, 1157, 1775, 2310, 2701, 2819, 2948
DinI GGCGCC 2 cut(s) 167, 592
DraI TTTAAA 5 cut(s) 538, 835, 1858, 2082, 2781
DraIII CACNNNGTG 1 cut(s) 420
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 3 cut(s) 3, 585, 845
EagI CGGCCG 2 cut(s) 585, 845
Eam1104I CTCTTC 2 cut(s) 215, 2904
EarI CTCTTC 2 cut(s) 215, 2904
EciI GGCGGA 3 cut(s) 230, 1184, 2782
Ecl136II GAGCTC 2 cut(s) 225, 634
EclXI CGGCCG 2 cut(s) 585, 845
Eco130I CCWWGG 6 cut(s) 776, 1543, 1998, 2038, 2258, 3096
Eco147I AGGCCT 3 cut(s) 398, 1210, 1542
Eco24I GRGCYC 3 cut(s) 227, 473, 636
Eco31I GGTCTC 2 cut(s) 1856, 2060
Eco32I GATATC 1 cut(s) 2035
Eco47I GGWCC 3 cut(s) 1972, 2495, 3093
Eco52I CGGCCG 2 cut(s) 585, 845
Eco53kI GAGCTC 2 cut(s) 225, 634
Eco57I CTGAAG 5 cut(s) 1233, 1997, 2394, 2442, 2457
Eco88I CYCGRG 3 cut(s) 191, 277, 972
Eco91I GGTNACC 1 cut(s) 986
EcoICRI GAGCTC 2 cut(s) 225, 634
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 3 cut(s) 773, 1972, 3093
EcoO65I GGTNACC 1 cut(s) 986
EcoRII CCWGG 7 cut(s) 75, 317, 393, 398, 891, 1375, 1617
EcoRV GATATC 1 cut(s) 2035
EcoT14I CCWWGG 6 cut(s) 776, 1543, 1998, 2038, 2258, 3096
EcoT22I ATGCAT 2 cut(s) 754, 1793
EcoT38I GRGCYC 3 cut(s) 227, 473, 636
EgeI GGCGCC 2 cut(s) 167, 592
EheI GGCGCC 2 cut(s) 167, 592
ErhI CCWWGG 6 cut(s) 776, 1543, 1998, 2038, 2258, 3096
FalI AAGNNNNNCTT 4 cut(s) 1265, 1297, 1764, 1796
FaqI GGGAC 1 cut(s) 825
FauI CCCGC 1 cut(s) 820
FbaI TGATCA 2 cut(s) 429, 2715
FblI GTMKAC 2 cut(s) 87, 348
FriOI GRGCYC 3 cut(s) 227, 473, 636
FseI GGCCGGCC 1 cut(s) 587
FspBI CTAG 6 cut(s) 344, 1206, 1740, 2027, 2175, 2991
GlaI GCGC 8 cut(s) 167, 203, 208, 334, 392, 592, 1512, 2447
GsaI CCCAGC 1 cut(s) 464
GsuI CTGGAG 3 cut(s) 1588, 2274, 2904
HaeII RGCGCY 4 cut(s) 169, 205, 594, 2449
HapII CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2834
HgaI GACGC 3 cut(s) 61, 352, 359
HhaI GCGC 8 cut(s) 168, 204, 209, 335, 393, 593, 1513, 2448
Hin1I GRCGYC 4 cut(s) 166, 351, 363, 591
Hin6I GCGC 8 cut(s) 166, 202, 207, 333, 391, 591, 1511, 2446
HinP1I GCGC 8 cut(s) 166, 202, 207, 333, 391, 591, 1511, 2446
HincII GTYRAC 2 cut(s) 88, 349
HindII GTYRAC 2 cut(s) 88, 349
HindIII AAGCTT 3 cut(s) 1194, 2541, 2845
HinfI GANTC 9 cut(s) 32, 182, 272, 1018, 1202, 1427, 1880, 2116, 2315
HpaII CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2834
Hpy166II GTNNAC 7 cut(s) 88, 349, 367, 889, 1691, 2814, 2877
Hpy188III TCNNGA 9 cut(s) 406, 509, 974, 1048, 1477, 1781, 1975, 2291, 2927
Hpy8I GTNNAC 7 cut(s) 88, 349, 367, 889, 1691, 2814, 2877
Hpy99I CGWCG 5 cut(s) 89, 332, 353, 359, 599
HpyCH4III ACNGT 6 cut(s) 265, 1092, 1592, 1732, 2104, 2818
HpyCH4IV ACGT 3 cut(s) 84, 950, 2140
HpySE526I ACGT 3 cut(s) 84, 950, 2140
Hsp92I GRCGYC 4 cut(s) 166, 351, 363, 591
HspAI GCGC 8 cut(s) 166, 202, 207, 333, 391, 591, 1511, 2446
KasI GGCGCC 2 cut(s) 165, 590
KroI GCCGGC 2 cut(s) 583, 587
KroNI GCCGGC 2 cut(s) 585, 589
Ksp22I TGATCA 2 cut(s) 429, 2715
KspI CCGCGG 1 cut(s) 2335
LguI GCTCTTC 2 cut(s) 215, 2904
LweI GCATC 8 cut(s) 150, 469, 556, 1138, 1778, 2101, 2362, 2638
MaeI CTAG 6 cut(s) 344, 1206, 1740, 2027, 2175, 2991
MaeII ACGT 3 cut(s) 84, 950, 2140
MaeIII GTNAC 7 cut(s) 259, 986, 1110, 1726, 1936, 2416, 2504
MboII GAAGA 9 cut(s) 232, 511, 811, 814, 1361, 2400, 2608, 2891, 3041
MfeI CAATTG 3 cut(s) 1644, 2121, 2732
MflI RGATCY 3 cut(s) 1353, 2241, 2935
MhlI GDGCHC 6 cut(s) 227, 473, 636, 2251, 2328, 2899
Mly113I GGCGCC 2 cut(s) 166, 591
MlyI GAGTC 4 cut(s) 176, 266, 1027, 2309
MmeI TCCRAC 1 cut(s) 156
Mph1103I ATGCAT 2 cut(s) 754, 1793
MroNI GCCGGC 2 cut(s) 583, 587
MroXI GAANNNNTTC 1 cut(s) 1752
MslI CAYNNNNRTG 2 cut(s) 215, 2826
MspA1I CMGCKG 4 cut(s) 138, 2334, 2396, 2492
MspI CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2834
MssI GTTTAAAC 1 cut(s) 2082
MteI GCGCNGCGC 1 cut(s) 205
MunI CAATTG 3 cut(s) 1644, 2121, 2732
Mva1269I GAATGC 1 cut(s) 1462
MvaI CCWGG 7 cut(s) 77, 319, 395, 400, 893, 1377, 1619
MvnI CGCG 5 cut(s) 207, 335, 455, 2334, 3080
NaeI GCCGGC 2 cut(s) 585, 589
NarI GGCGCC 2 cut(s) 166, 591
NciI CCSGG 3 cut(s) 163, 278, 279
NgoMIV GCCGGC 2 cut(s) 583, 587
NlaIV GGNNCC 7 cut(s) 130, 167, 582, 592, 774, 2338, 2937
NmeAIII GCCGAG 2 cut(s) 739, 823
NmuCI GTSAC 5 cut(s) 259, 986, 1726, 1936, 2504
NsiI ATGCAT 2 cut(s) 754, 1793
NspI RCATGY 3 cut(s) 1502, 1754, 2825
NspV TTCGAA 1 cut(s) 1238
PaeR7I CTCGAG 1 cut(s) 191
PagI TCATGA 2 cut(s) 508, 1476
PceI AGGCCT 3 cut(s) 398, 1210, 1542
PciI ACATGT 1 cut(s) 1750
PciSI GCTCTTC 2 cut(s) 215, 2904
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PctI GAATGC 1 cut(s) 1462
PdiI GCCGGC 2 cut(s) 585, 589
PdmI GAANNNNTTC 1 cut(s) 1752
PfeI GAWTC 5 cut(s) 32, 1202, 1427, 1880, 2116
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 1277
PleI GAGTC 4 cut(s) 176, 266, 1026, 2309
PluTI GGCGCC 2 cut(s) 169, 594
PmeI GTTTAAAC 1 cut(s) 2082
PpsI GAGTC 4 cut(s) 176, 266, 1026, 2309
PpuMI RGGWCCY 2 cut(s) 1972, 3093
PscI ACATGT 1 cut(s) 1750
Psp124BI GAGCTC 2 cut(s) 227, 636
Psp1406I AACGTT 1 cut(s) 950
Psp5II RGGWCCY 2 cut(s) 1972, 3093
Psp6I CCWGG 7 cut(s) 75, 317, 393, 398, 891, 1375, 1617
PspEI GGTNACC 1 cut(s) 986
PspFI CCCAGC 1 cut(s) 460
PspGI CCWGG 7 cut(s) 75, 317, 393, 398, 891, 1375, 1617
PspN4I GGNNCC 7 cut(s) 130, 167, 582, 592, 774, 2338, 2937
PspPPI RGGWCCY 2 cut(s) 1972, 3093
PspXI VCTCGAGB 1 cut(s) 191
PstI CTGCAG 3 cut(s) 299, 1666, 1768
PstNI CAGNNNCTG 2 cut(s) 2399, 2492
PsuI RGATCY 3 cut(s) 1353, 2241, 2935
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 2 cut(s) 2396, 2492
RigI GGCCGGCC 1 cut(s) 587
RsaI GTAC 8 cut(s) 745, 890, 1158, 1776, 2311, 2702, 2820, 2949
RsaNI GTAC 8 cut(s) 744, 889, 1157, 1775, 2310, 2701, 2819, 2948
RseI CAYNNNNRTG 2 cut(s) 215, 2826
SacI GAGCTC 2 cut(s) 227, 636
SacII CCGCGG 1 cut(s) 2335
SalI GTCGAC 2 cut(s) 86, 347
SapI GCTCTTC 2 cut(s) 215, 2904
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 4 cut(s) 176, 266, 1027, 2309
SduI GDGCHC 6 cut(s) 227, 473, 636, 2251, 2328, 2899
SfaNI GCATC 8 cut(s) 150, 469, 556, 1138, 1778, 2101, 2362, 2638
SfcI CTRYAG 5 cut(s) 295, 1357, 1662, 1764, 2103
SfoI GGCGCC 2 cut(s) 167, 592
Sfr274I CTCGAG 1 cut(s) 191
Sfr303I CCGCGG 1 cut(s) 2335
SfuI TTCGAA 1 cut(s) 1238
SgrBI CCGCGG 1 cut(s) 2335
SinI GGWCC 3 cut(s) 1972, 2495, 3093
SlaI CTCGAG 1 cut(s) 191
SmaI CCCGGG 1 cut(s) 279
SmiMI CAYNNNNRTG 2 cut(s) 215, 2826
SmlI CTYRAG 4 cut(s) 191, 869, 921, 1889
SmoI CTYRAG 4 cut(s) 191, 869, 921, 1889
SseBI AGGCCT 3 cut(s) 398, 1210, 1542
SspDI GGCGCC 2 cut(s) 165, 590
SspMI CTAG 6 cut(s) 344, 1206, 1740, 2027, 2175, 2991
SstI GAGCTC 2 cut(s) 227, 636
StuI AGGCCT 3 cut(s) 398, 1210, 1542
StyI CCWWGG 6 cut(s) 776, 1543, 1998, 2038, 2258, 3096
TaaI ACNGT 6 cut(s) 265, 1092, 1592, 1732, 2104, 2818
TaiI ACGT 3 cut(s) 87, 953, 2143
TaqI TCGA 8 cut(s) 87, 192, 270, 348, 357, 405, 441, 1238
TatI WGTACW 5 cut(s) 743, 1156, 1774, 2700, 2818
TauI GCSGC 5 cut(s) 141, 207, 317, 899, 2337
TfiI GAWTC 5 cut(s) 32, 1202, 1427, 1880, 2116
TscAI CASTG 5 cut(s) 251, 2107, 2821, 2921, 3060
TseFI GTSAC 5 cut(s) 259, 986, 1726, 1936, 2504
Tsp45I GTSAC 5 cut(s) 259, 986, 1726, 1936, 2504
TspDTI ATGAA 9 cut(s) 233, 497, 1329, 1566, 1697, 1893, 2025, 2312, 2441
TspGWI ACGGA 2 cut(s) 247, 2972
TspMI CCCGGG 1 cut(s) 277
TspRI CASTG 5 cut(s) 251, 2107, 2821, 2921, 3060
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 1277
VpaK11BI GGWCC 3 cut(s) 1972, 2495, 3093
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 3 cut(s) 731, 1386, 1489
XceI RCATGY 3 cut(s) 1502, 1754, 2825
XhoI CTCGAG 1 cut(s) 191
XmaI CCCGGG 1 cut(s) 277
XmiI GTMKAC 2 cut(s) 87, 348
XmnI GAANNNNTTC 1 cut(s) 1752
XspI CTAG 6 cut(s) 344, 1206, 1740, 2027, 2175, 2991
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 2 cut(s) 754, 1793
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.