Rh2AG294600

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0002828
Physical Location & Seq
Forward (+)
36317631 .. 36318113
483 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG294600.1

Sequence Viewer

Length: 483 bp
ATGACAGCCGTTAAGGATGCTTGTAGGGTAGCTGTGCTTCAAAAGAAACCACGGCTTGTGGAAGCTATGTACTTCTGTGAGTTGATCACGCCAACTGAACAATTGGGTAATATGTATGTTGTGCTTGGACGAAGGCGGACGAAAGTTTTAAAGGAGGAAATGCAGGAAGGATCTGCATTGTTCACTGTACATGCCTATGTGCCGGTTTCTGAAAGCTTTGGTTTTGCGGATGAGCTTAGAAGGTGGACTGCTGGAGCTGCAAGTGCTCTTCTTGTGCTTAGCCACTGGGAAGCTCTTGATAAGGATCCTTTCTTTGTACCAAAAACGGATGAGGAAAAAGAGGAGTTTGGTGATGGTTCTAGTGTGCCTCCAAATACAGCAAGAAAACTGATAAATGCTGTAAGACTGCATAAGGGTCTTCTAGTGGAGGAAAAAGTAGTGCAGCATACGACAAAGCAGAGGACCTTGGCTCGTAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

160

Amino Acids

18.04

Weight (kDa)

8.89

Isoelectric Point (pI)

49.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFG_C PF00679 18 - 103 5e-17 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 136, 227
AclWI GGATC 3 cut(s) 178, 299, 312
AfaI GTAC 3 cut(s) 71, 189, 318
AgsI TTSAA 1 cut(s) 41
AluBI AGCT 6 cut(s) 32, 65, 216, 235, 257, 293
AluI AGCT 6 cut(s) 32, 65, 216, 235, 257, 293
Alw21I GWGCWC 1 cut(s) 268
AlwI GGATC 3 cut(s) 178, 299, 312
ApeKI GCWGC 2 cut(s) 257, 442
AspS9I GGNCC 1 cut(s) 462
AsuHPI GGTGA 1 cut(s) 362
AvaII GGWCC 1 cut(s) 462
BamHI GGATCC 1 cut(s) 304
BarI GAAGNNNNNNTAC 2 cut(s) 21, 53
BbsI GAAGAC 1 cut(s) 410
Bbv12I GWGCWC 1 cut(s) 268
BbvI GCAGC 2 cut(s) 244, 454
BccI CCATC 1 cut(s) 347
BceAI ACGGC 1 cut(s) 68
BclI TGATCA 1 cut(s) 84
BfaI CTAG 2 cut(s) 360, 422
BisI GCNGC 2 cut(s) 258, 443
BlpI GCTNAGC 1 cut(s) 278
BlsI GCNGC 2 cut(s) 259, 444
Bme18I GGWCC 1 cut(s) 462
BmgT120I GGNCC 1 cut(s) 462
BmiI GGNNCC 1 cut(s) 306
BmrI ACTGGG 1 cut(s) 295
BmsI GCATC 1 cut(s) 7
BmuI ACTGGG 1 cut(s) 295
BpiI GAAGAC 1 cut(s) 410
BpmI CTGGAG 1 cut(s) 273
Bpu1102I GCTNAGC 1 cut(s) 278
BsaBI GATNNNNATC 1 cut(s) 303
BsaJI CCNNGG 2 cut(s) 50, 465
Bse118I RCCGGY 1 cut(s) 202
Bse1I ACTGG 1 cut(s) 290
Bse8I GATNNNNATC 1 cut(s) 303
BseDI CCNNGG 2 cut(s) 50, 465
BseGI GGATG 3 cut(s) 22, 235, 334
BseJI GATNNNNATC 1 cut(s) 303
BseNI ACTGG 1 cut(s) 290
BseRI GAGGAG 1 cut(s) 356
BseXI GCAGC 2 cut(s) 244, 454
BsgI GTGCAG 1 cut(s) 461
BsiHKAI GWGCWC 1 cut(s) 268
BsiSI CCGG 1 cut(s) 203
Bsp1286I GDGCHC 1 cut(s) 268
Bsp1407I TGTACA 1 cut(s) 187
Bsp143I GATC 3 cut(s) 84, 170, 304
Bsp1720I GCTNAGC 1 cut(s) 278
BspACI CCGC 2 cut(s) 136, 227
BspLI GGNNCC 1 cut(s) 306
BspPI GGATC 3 cut(s) 178, 299, 312
BspQI GCTCTTC 1 cut(s) 273
BsrFI RCCGGY 1 cut(s) 202
BsrGI TGTACA 1 cut(s) 187
BsrI ACTGG 1 cut(s) 290
BssAI RCCGGY 1 cut(s) 202
BssECI CCNNGG 2 cut(s) 50, 465
BssMI GATC 3 cut(s) 84, 170, 304
BssT1I CCWWGG 1 cut(s) 465
Bst4CI ACNGT 1 cut(s) 187
Bst6I CTCTTC 1 cut(s) 273
BstAUI TGTACA 1 cut(s) 187
BstDEI CTNAG 2 cut(s) 236, 278
BstDSI CCRYGG 1 cut(s) 50
BstF5I GGATG 3 cut(s) 22, 235, 334
BstKTI GATC 3 cut(s) 87, 173, 307
BstMBI GATC 3 cut(s) 84, 170, 304
BstMWI GCNNNNNNNGC 2 cut(s) 257, 263
BstNSI RCATGY 1 cut(s) 194
BstV1I GCAGC 2 cut(s) 244, 454
BstV2I GAAGAC 1 cut(s) 410
BstX2I RGATCY 2 cut(s) 170, 304
BstYI RGATCY 2 cut(s) 170, 304
BtgI CCRYGG 1 cut(s) 50
BtsCI GGATG 3 cut(s) 22, 235, 334
BtsIMutI CAGTG 2 cut(s) 183, 283
Cfr10I RCCGGY 1 cut(s) 202
Cfr13I GGNCC 1 cut(s) 462
Csp6I GTAC 3 cut(s) 70, 188, 317
CviAII CATG 1 cut(s) 191
CviQI GTAC 3 cut(s) 70, 188, 317
DdeI CTNAG 2 cut(s) 236, 278
DpnI GATC 3 cut(s) 86, 172, 306
DpnII GATC 3 cut(s) 84, 170, 304
DraI TTTAAA 1 cut(s) 150
Eam1104I CTCTTC 1 cut(s) 273
EarI CTCTTC 1 cut(s) 273
EciI GGCGGA 1 cut(s) 151
Eco130I CCWWGG 1 cut(s) 465
Eco47I GGWCC 1 cut(s) 462
EcoO109I RGGNCCY 1 cut(s) 462
EcoT14I CCWWGG 1 cut(s) 465
ErhI CCWWGG 1 cut(s) 465
FaeI CATG 1 cut(s) 194
FaiI YATR 7 cut(s) 68, 113, 117, 192, 198, 411, 447
FatI CATG 1 cut(s) 190
FbaI TGATCA 1 cut(s) 84
Fnu4HI GCNGC 2 cut(s) 258, 443
FokI GGATG 3 cut(s) 29, 242, 341
Fsp4HI GCNGC 2 cut(s) 258, 443
FspBI CTAG 2 cut(s) 360, 422
GluI GCNGC 2 cut(s) 258, 443
GsuI CTGGAG 1 cut(s) 273
HapII CCGG 1 cut(s) 203
Hin1II CATG 1 cut(s) 194
HindIII AAGCTT 1 cut(s) 214
HpaII CCGG 1 cut(s) 203
HphI GGTGA 1 cut(s) 362
Hpy166II GTNNAC 2 cut(s) 183, 246
Hpy188I TCNGA 1 cut(s) 211
Hpy188III TCNNGA 1 cut(s) 296
Hpy8I GTNNAC 2 cut(s) 183, 246
HpyAV CCTTC 3 cut(s) 126, 161, 234
HpyCH4III ACNGT 1 cut(s) 187
HpyCH4V TGCA 5 cut(s) 163, 176, 260, 409, 442
HpyF10VI GCNNNNNNNGC 2 cut(s) 257, 263
HpyF3I CTNAG 2 cut(s) 236, 278
Hsp92II CATG 1 cut(s) 194
Ksp22I TGATCA 1 cut(s) 84
Kzo9I GATC 3 cut(s) 84, 170, 304
LguI GCTCTTC 1 cut(s) 273
LmnI GCTCC 1 cut(s) 254
LpnPI CCDG 4 cut(s) 149, 216, 237, 271
Lsp1109I GCAGC 2 cut(s) 244, 454
LweI GCATC 1 cut(s) 7
MaeI CTAG 2 cut(s) 360, 422
MalI GATC 3 cut(s) 86, 172, 306
MboI GATC 3 cut(s) 84, 170, 304
MboII GAAGA 2 cut(s) 260, 410
MfeI CAATTG 1 cut(s) 101
MflI RGATCY 2 cut(s) 170, 304
MhlI GDGCHC 1 cut(s) 268
MluCI AATT 1 cut(s) 101
MnlI CCTC 6 cut(s) 148, 325, 334, 378, 421, 453
MseI TTAA 2 cut(s) 12, 149
MslI CAYNNNNRTG 1 cut(s) 195
MspI CCGG 1 cut(s) 203
MunI CAATTG 1 cut(s) 101
MwoI GCNNNNNNNGC 2 cut(s) 257, 263
NdeII GATC 3 cut(s) 84, 170, 304
NlaIII CATG 1 cut(s) 194
NlaIV GGNNCC 1 cut(s) 306
NspI RCATGY 1 cut(s) 194
PciSI GCTCTTC 1 cut(s) 273
PkrI GCNGC 2 cut(s) 259, 444
PpuMI RGGWCCY 1 cut(s) 462
Psp5II RGGWCCY 1 cut(s) 462
PspN4I GGNNCC 1 cut(s) 306
PspPI GGNCC 1 cut(s) 462
PspPPI RGGWCCY 1 cut(s) 462
PsuI RGATCY 2 cut(s) 170, 304
RsaI GTAC 3 cut(s) 71, 189, 318
RsaNI GTAC 3 cut(s) 70, 188, 317
RseI CAYNNNNRTG 1 cut(s) 195
SapI GCTCTTC 1 cut(s) 273
SaqAI TTAA 2 cut(s) 12, 149
SatI GCNGC 2 cut(s) 258, 443
Sau3AI GATC 3 cut(s) 84, 170, 304
Sau96I GGNCC 1 cut(s) 462
SduI GDGCHC 1 cut(s) 268
SetI ASST 8 cut(s) 34, 67, 218, 237, 245, 259, 295, 467
SfaNI GCATC 1 cut(s) 7
SinI GGWCC 1 cut(s) 462
SmiMI CAYNNNNRTG 1 cut(s) 195
Sse9I AATT 1 cut(s) 101
SsiI CCGC 2 cut(s) 136, 227
SspMI CTAG 2 cut(s) 360, 422
StyI CCWWGG 1 cut(s) 465
TaaI ACNGT 1 cut(s) 187
TasI AATT 1 cut(s) 101
TatI WGTACW 2 cut(s) 69, 187
Tru1I TTAA 2 cut(s) 12, 149
Tru9I TTAA 2 cut(s) 12, 149
TscAI CASTG 2 cut(s) 190, 290
TseI GCWGC 2 cut(s) 257, 442
TspGWI ACGGA 1 cut(s) 341
TspRI CASTG 2 cut(s) 190, 290
VpaK11BI GGWCC 1 cut(s) 462
XceI RCATGY 1 cut(s) 194
XspI CTAG 2 cut(s) 360, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.