FvH4_4g04801

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002828
Physical Location & Seq
Forward (+)
4187587 .. 4188524
938 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04801.t1

Sequence Viewer

Length: 675 bp
ATGGAGGCTGTGGAGAGTGATATTTTGTCTAGCGGTGAGACTGACAAGGATCGGGCTGAGAAATGCTCCTCTCATGTTTCTGAGATATTAGGACTGGTGGATGCTTCTGACAATGAGAATGCAGCTGCTGAGACATCTTCAGAAGTAACTGAAGCACTGTATGATGAAGCAAAGAGCCTCAAGAGCAGTGTTTTTTCCAGATTTCAACAGGCAACAGCAGCTGGACCTTTATGTGACGAGCCTATGTGGGGTTTGGCATTTGTGGTTGAAGCTTACATTTCACCATTAGCTGCTCATTCTGATAATTTGAAAACAACCGACCACCAGGCAGACCAAAGTTTTCAAGGAAGAAATGCAGGAAGGCTCCCATTGTTCACTGTACATGCCTATTTGCCGGTTTCTGAAAGCTTTGGTTTTGCGGAGGATCTTAGAAGATGGACTGCTGGAGCTGCAAGTGCTCATCTTGTGCTCAGCCACTGGGAAGCACTTGATAATGATCCTTTCTTTGTACCAAAAACGGATGAGGAGAGAGAAGAGTTTGGCGACGGTTCTAGTGTGCGTCCAAATACAGCTAGAAAACTGATAAATGTTGTAAGAAAGCAGAAGGGTCTTCCTGTGGAGGAAAAAGTAGTGCAGCACGCGACAAAGCAGAGGACCTTGGCTCGTAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

225

Amino Acids

24.58

Weight (kDa)

5.04

Isoelectric Point (pI)

43.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EFG_C PF00679 125 - 167 1.2e-07 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 641
AciI CCGC 2 cut(s) 33, 419
AclWI GGATC 3 cut(s) 57, 432, 491
AcuI CTGAAG 2 cut(s) 123, 171
AfaI GTAC 2 cut(s) 381, 510
AfiI CCNNNNNNNGG 1 cut(s) 248
AgsI TTSAA 4 cut(s) 206, 269, 310, 344
AjnI CCWGG 1 cut(s) 324
AluBI AGCT 7 cut(s) 125, 221, 272, 290, 408, 449, 572
AluI AGCT 7 cut(s) 125, 221, 272, 290, 408, 449, 572
Alw21I GWGCWC 2 cut(s) 460, 471
Alw26I GTCTC 2 cut(s) 32, 125
AlwI GGATC 3 cut(s) 57, 432, 491
AlwNI CAGNNNCTG 3 cut(s) 128, 221, 477
ApeKI GCWGC 6 cut(s) 122, 125, 218, 290, 449, 634
AspS9I GGNCC 2 cut(s) 224, 654
AsuHPI GGTGA 2 cut(s) 47, 273
AvaII GGWCC 2 cut(s) 224, 654
BbsI GAAGAC 1 cut(s) 602
Bbv12I GWGCWC 2 cut(s) 460, 471
BbvI GCAGC 6 cut(s) 112, 134, 230, 277, 436, 646
BccI CCATC 1 cut(s) 429
BciT130I CCWGG 1 cut(s) 326
BcoDI GTCTC 2 cut(s) 32, 125
BfaI CTAG 3 cut(s) 30, 552, 573
BisI GCNGC 6 cut(s) 123, 126, 219, 291, 450, 635
BlpI GCTNAGC 1 cut(s) 470
BlsI GCNGC 6 cut(s) 124, 127, 220, 292, 451, 636
Bme1390I CCNGG 1 cut(s) 326
Bme18I GGWCC 2 cut(s) 224, 654
BmgT120I GGNCC 2 cut(s) 224, 654
BmiI GGNNCC 1 cut(s) 365
BmrFI CCNGG 1 cut(s) 326
BmrI ACTGGG 1 cut(s) 487
BmsI GCATC 1 cut(s) 91
BmuI ACTGGG 1 cut(s) 487
BpiI GAAGAC 1 cut(s) 602
BplI GAGNNNNNCTC 2 cut(s) 50, 82
BpmI CTGGAG 1 cut(s) 465
Bpu1102I GCTNAGC 1 cut(s) 470
BpuEI CTTGAG 1 cut(s) 164
BsaBI GATNNNNATC 1 cut(s) 495
BsaJI CCNNGG 1 cut(s) 657
Bsc4I CCNNNNNNNGG 1 cut(s) 248
Bse118I RCCGGY 1 cut(s) 394
Bse1I ACTGG 2 cut(s) 99, 482
Bse8I GATNNNNATC 1 cut(s) 495
BseBI CCWGG 1 cut(s) 326
BseDI CCNNGG 1 cut(s) 657
BseGI GGATG 2 cut(s) 106, 526
BseJI GATNNNNATC 1 cut(s) 495
BseLI CCNNNNNNNGG 1 cut(s) 248
BseMII CTCAG 4 cut(s) 48, 72, 120, 484
BseNI ACTGG 2 cut(s) 99, 482
BseRI GAGGAG 2 cut(s) 58, 539
BseXI GCAGC 6 cut(s) 112, 134, 230, 277, 436, 646
BsgI GTGCAG 1 cut(s) 653
Bsh1236I CGCG 1 cut(s) 641
BsiHKAI GWGCWC 2 cut(s) 460, 471
BsiSI CCGG 1 cut(s) 395
BslI CCNNNNNNNGG 1 cut(s) 248
BsmAI GTCTC 2 cut(s) 32, 125
BsmI GAATGC 1 cut(s) 124
Bsp1286I GDGCHC 2 cut(s) 460, 471
Bsp1407I TGTACA 1 cut(s) 379
Bsp143I GATC 3 cut(s) 49, 424, 496
Bsp1720I GCTNAGC 1 cut(s) 470
BspACI CCGC 2 cut(s) 33, 419
BspCNI CTCAG 4 cut(s) 49, 73, 121, 483
BspFNI CGCG 1 cut(s) 641
BspLI GGNNCC 1 cut(s) 365
BspPI GGATC 3 cut(s) 57, 432, 491
BsrFI RCCGGY 1 cut(s) 394
BsrGI TGTACA 1 cut(s) 379
BsrI ACTGG 2 cut(s) 99, 482
BssAI RCCGGY 1 cut(s) 394
BssECI CCNNGG 1 cut(s) 657
BssMI GATC 3 cut(s) 49, 424, 496
BssT1I CCWWGG 1 cut(s) 657
Bst2UI CCWGG 1 cut(s) 326
Bst4CI ACNGT 3 cut(s) 159, 379, 548
Bst6I CTCTTC 1 cut(s) 528
BstAUI TGTACA 1 cut(s) 379
BstC8I GCNNGC 1 cut(s) 639
BstDEI CTNAG 5 cut(s) 57, 81, 129, 428, 470
BstF5I GGATG 2 cut(s) 106, 526
BstFNI CGCG 1 cut(s) 641
BstKTI GATC 3 cut(s) 52, 427, 499
BstMAI GTCTC 2 cut(s) 32, 125
BstMBI GATC 3 cut(s) 49, 424, 496
BstMWI GCNNNNNNNGC 4 cut(s) 183, 218, 449, 455
BstNI CCWGG 1 cut(s) 326
BstNSI RCATGY 1 cut(s) 386
BstSCI CCNGG 1 cut(s) 324
BstUI CGCG 1 cut(s) 641
BstV1I GCAGC 6 cut(s) 112, 134, 230, 277, 436, 646
BstV2I GAAGAC 1 cut(s) 602
BstX2I RGATCY 1 cut(s) 424
BstYI RGATCY 1 cut(s) 424
BtsCI GGATG 2 cut(s) 106, 526
BtsI GCAGTG 1 cut(s) 193
BtsIMutI CAGTG 4 cut(s) 155, 193, 375, 475
Cac8I GCNNGC 1 cut(s) 639
CaiI CAGNNNCTG 3 cut(s) 128, 221, 477
Cfr10I RCCGGY 1 cut(s) 394
Cfr13I GGNCC 2 cut(s) 224, 654
CseI GACGC 1 cut(s) 548
Csp6I GTAC 2 cut(s) 380, 509
CviAII CATG 2 cut(s) 74, 383
CviQI GTAC 2 cut(s) 380, 509
DdeI CTNAG 5 cut(s) 57, 81, 129, 428, 470
DpnI GATC 3 cut(s) 51, 426, 498
DpnII GATC 3 cut(s) 49, 424, 496
Eam1104I CTCTTC 1 cut(s) 528
EarI CTCTTC 1 cut(s) 528
Eco130I CCWWGG 1 cut(s) 657
Eco47I GGWCC 2 cut(s) 224, 654
Eco57I CTGAAG 2 cut(s) 123, 171
EcoO109I RGGNCCY 1 cut(s) 654
EcoRII CCWGG 1 cut(s) 324
EcoT14I CCWWGG 1 cut(s) 657
ErhI CCWWGG 1 cut(s) 657
FaeI CATG 2 cut(s) 77, 386
FaiI YATR 5 cut(s) 75, 162, 232, 245, 384
FatI CATG 2 cut(s) 73, 382
Fnu4HI GCNGC 6 cut(s) 123, 126, 219, 291, 450, 635
FokI GGATG 2 cut(s) 113, 533
Fsp4HI GCNGC 6 cut(s) 123, 126, 219, 291, 450, 635
FspBI CTAG 3 cut(s) 30, 552, 573
GluI GCNGC 6 cut(s) 123, 126, 219, 291, 450, 635
GsuI CTGGAG 1 cut(s) 465
HapII CCGG 1 cut(s) 395
HgaI GACGC 1 cut(s) 548
Hin1II CATG 2 cut(s) 77, 386
HindIII AAGCTT 2 cut(s) 270, 406
HpaII CCGG 1 cut(s) 395
HphI GGTGA 2 cut(s) 47, 273
Hpy166II GTNNAC 1 cut(s) 375
Hpy188I TCNGA 5 cut(s) 82, 109, 142, 301, 403
Hpy188III TCNNGA 2 cut(s) 181, 198
Hpy8I GTNNAC 1 cut(s) 375
Hpy99I CGWCG 1 cut(s) 548
HpyAV CCTTC 2 cut(s) 354, 598
HpyCH4III ACNGT 3 cut(s) 159, 379, 548
HpyCH4V TGCA 4 cut(s) 122, 356, 452, 634
HpyF10VI GCNNNNNNNGC 4 cut(s) 183, 218, 449, 455
HpyF3I CTNAG 5 cut(s) 57, 81, 129, 428, 470
Hsp92II CATG 2 cut(s) 77, 386
Kzo9I GATC 3 cut(s) 49, 424, 496
LmnI GCTCC 3 cut(s) 71, 369, 446
Lsp1109I GCAGC 6 cut(s) 112, 134, 230, 277, 436, 646
LweI GCATC 1 cut(s) 91
MaeI CTAG 3 cut(s) 30, 552, 573
MaeIII GTNAC 2 cut(s) 145, 233
MalI GATC 3 cut(s) 51, 426, 498
MboI GATC 3 cut(s) 49, 424, 496
MboII GAAGA 5 cut(s) 129, 360, 444, 545, 602
MflI RGATCY 1 cut(s) 424
MhlI GDGCHC 2 cut(s) 460, 471
MluCI AATT 1 cut(s) 304
MnlI CCTC 6 cut(s) 79, 188, 415, 517, 613, 645
MspA1I CMGCKG 2 cut(s) 125, 221
MspI CCGG 1 cut(s) 395
MspR9I CCNGG 1 cut(s) 326
Mva1269I GAATGC 1 cut(s) 124
MvaI CCWGG 1 cut(s) 326
MvnI CGCG 1 cut(s) 641
MwoI GCNNNNNNNGC 4 cut(s) 183, 218, 449, 455
NdeII GATC 3 cut(s) 49, 424, 496
NlaIII CATG 2 cut(s) 77, 386
NlaIV GGNNCC 1 cut(s) 365
NmuCI GTSAC 1 cut(s) 233
NspI RCATGY 1 cut(s) 386
PctI GAATGC 1 cut(s) 124
PkrI GCNGC 6 cut(s) 124, 127, 220, 292, 451, 636
PpuMI RGGWCCY 1 cut(s) 654
Psp5II RGGWCCY 1 cut(s) 654
Psp6I CCWGG 1 cut(s) 324
PspGI CCWGG 1 cut(s) 324
PspN4I GGNNCC 1 cut(s) 365
PspPI GGNCC 2 cut(s) 224, 654
PspPPI RGGWCCY 1 cut(s) 654
PstNI CAGNNNCTG 3 cut(s) 128, 221, 477
PsuI RGATCY 1 cut(s) 424
PvuII CAGCTG 2 cut(s) 125, 221
RsaI GTAC 2 cut(s) 381, 510
RsaNI GTAC 2 cut(s) 380, 509
SatI GCNGC 6 cut(s) 123, 126, 219, 291, 450, 635
Sau3AI GATC 3 cut(s) 49, 424, 496
Sau96I GGNCC 2 cut(s) 224, 654
ScrFI CCNGG 1 cut(s) 326
SduI GDGCHC 2 cut(s) 460, 471
SetI ASST 9 cut(s) 127, 223, 229, 274, 292, 410, 451, 574, 659
SfaNI GCATC 1 cut(s) 91
SinI GGWCC 2 cut(s) 224, 654
SmlI CTYRAG 1 cut(s) 179
SmoI CTYRAG 1 cut(s) 179
Sse9I AATT 1 cut(s) 304
SsiI CCGC 2 cut(s) 33, 419
SspMI CTAG 3 cut(s) 30, 552, 573
StyD4I CCNGG 1 cut(s) 324
StyI CCWWGG 1 cut(s) 657
TaaI ACNGT 3 cut(s) 159, 379, 548
TasI AATT 1 cut(s) 304
TatI WGTACW 1 cut(s) 379
TscAI CASTG 4 cut(s) 162, 193, 382, 482
TseFI GTSAC 1 cut(s) 233
TseI GCWGC 6 cut(s) 122, 125, 218, 290, 449, 634
Tsp45I GTSAC 1 cut(s) 233
TspDTI ATGAA 1 cut(s) 180
TspGWI ACGGA 1 cut(s) 533
TspRI CASTG 4 cut(s) 162, 193, 382, 482
VpaK11BI GGWCC 2 cut(s) 224, 654
XceI RCATGY 1 cut(s) 386
XspI CTAG 3 cut(s) 30, 552, 573
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.