Rroxscaffold_2G00121910

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002828
Physical Location & Seq
Reverse (-)
54725782 .. 54728682
2901 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00121910.1

Sequence Viewer

Length: 2868 bp
ATGAAGAGCTCCTCCATCGCCCTCCGCCACCGCGACCACTCCGTCACCGTCATCGACTCGCCGGGCCACATGGACTTCTTCAGCGAAGTCTCCACCGCCGCCAGGCTCAGCGACGGCGCGTTGATCCTAGTCGACGCCGTGGAGGGCGCCTGGATCGAGAAGCTCACGCCGTGCTTGGTGATCAACAAGATCGATAGGTTGATCGCCGAGCTGGGAATGAGCCCTATGGATGCGTATGTAAAGTTGTTGAAAATTGTTCATGAGGTTAATAACATTGTTAGTGCTTTTAAATCTGTGAAATACTTGAACGATGTGGGTGCCATTCTTTCGGTGCCGGCCAGCGCCGACGAGTTGGCGTTTGAGGAGATTGAGGATGATGAGGAGCTCATGTTTCAGCCCCAGAAGGGCAATGTGGCCTTTGTGTGTGCTTTGGATGGTTGGGGGTTTAGTATTAGGCAATTTGCTGAGTTTTATGAGGCGAAATTCGGGTGGAGTACTAATGCATTGCAGAAGGCTTTATGGGGGCCTTGGTATTTTAATCACAAGGAGAAGAAGATTGTGGGACAGAAGGGTGTGGCGGGTTTGGAAAAGGCTCGGCCCATGTTTGTTGAGTTTGTGCTTGAGCCGGTTTGGAGTGTGTACCAGGCGGCTTTGAAGGAGAAGGAGGAGGCTGAAGTGATGGTTCAGAAGGTGATTAGAACGTTTAATTTGACTATACCACCTCGGGATTTGAAGGGTGACCCAAAAGGTATGGTTCAGGCTGTGATGAGTCGGTGGCTTCCTCTGCATGAGGCTATCTTGAACATGGTTATTAGGTGTATGCCTGACCCGATTGCAGCACAGTCATTTCGGATTACAAGGTTACTTCCAAAGAGAGAGGTTGTGGGAGATGGGGTTGATGCTAATGTACTTGCAGAGGTGGAGCTTGTGAGAAGTTCGGTGGAAGCTTCTGATTCTAGGCCTGAAGCTCCTTGTGTTGTTTTTGTTTCGAAAATGTTTGCTGTGCCAACGAAAACACTTCCAAAGTATGGCTTGGATGGGGAGATTTTGGATAATACATCTGATGAAGGTGAATTGGATGAGTGCTTCCTTGCATTTGCAAGGATCTATAGTGGGGTTCTTCGTCCTGGTGAGAAAATTTATGTGCTTTCAGCTTTATATGATCCCTTAAAAGGGGAATCAATGCAGAAGCATATTCAGGTGGCTAAGTTGTATCTCATGATGGGCCAAAATTTACAGCATGTGCCAGAGGCGCAGGCAGGGGATATTGTGGCAATCCGAGGCCTTGGGCAGCATATACTGAAAACTGCAACACTTTCATCAACAAAGAACTGTTGGCCTTTCTCCAGTATGTCATTCCAGGTTTCTCCCACTCTGAAAGTTGCAATTGAGCCATCTGATCCTGCAGATATGGGTGCATTGATGAAGGGTTTACGGCTACTGAACAGGGCAGACCCTTTTGTAGAGGTCACGGTTTCTGCTAGAGGAGAACATGTTCTGTCTGCTGCAGGAGAAGTACATCTTGAAAGATGCATAAAAGATTTGAAGGATAGATTTGCAAGGGTAGGCTTGGAAGTGTCTGAGCCTCTTGTGTCTTTTAAAGAGACCATTTTGGGAGATCAATCCACACTTGAGAACTTAAAATCCTTTGTTGCCAGTTCAGAATATGTTGAGAAGGTGACACAAAATGGAAGGTGTGTTGTTCGGGTGAAGGTCCTGAAGCTCCCACCTTCTCTAACCAAGGTGATTGATGAAAGTTCTGATATTCTAGCAGATATCCTTGGAGGCAAGGCTGCCCAGACAGGAGAGACCAATAAAAGTTTAAACACACAGGTTGCAAGCACTGTAGAGGATGCAAATCCAATTGAAACACTAAGGAAACGTATGATGGAAGCTGTGGAAAGTGATATTTTGTCTAGTGGTGAGACTGACAAAGATCGGGCTGAGAAATGTAAAGTCCAATGGCAAAAACTTCTTAAAAGGATCTGGGCACTTGGCCCTTGGCATATTGGCCCTAACATCCTACTTGCTCCAGAAATGAAAGGAAGGGATACTGACTCCTCTGTGCTCATCCGCGGCTCCTCTCATGTTTCTGAGAAATTAGGATTGGTGGATGCTTCTGGCAATGACAATGACAATGATAATGCAGCTGTTGACACATCTTCAGAAGTAACTGAAGCATTGTATGCTGAAGCAGAGCGCCTTCAGAGCAGCGTTTTATCTGGGTTTCAACTGGCAACAGCAGCTGGACCTTTATGTGACGAGCCTATGTGGGGTTTAGCATTTGTGGTTGAAGCTTACATTTCACCATTAGCTGCTCAGTCTGATGAATTAGAAACCTCCCACCAGCAACCTGAGCAATATGGTATCTTCACAGGACAGGTAATGACAGCCGTTAAGGATGCTTGTAGAGTAGCTGTGCTTCAAAAGAAACCACGGCTTGTGGAAGCTATGTACTTCTGTGAGTTGATCACGCCAACTGAACAATTGGGTAATATGTATGCTGTGCTTGGACGAAGGCGGACGAAAGTTTTAAAGGAGGAAATGCAGGAAGGCTCTGCATTGTTCACTATACATGCCTATGTGCCGGTTTCTGAAAGCTTTGGTTCTGCGGATGAGCTTAGAAGGTGGACTGCTAGAGCTGCAAGTGCTCTTCTTGTGCTTAGCCACTGGGAAGCTCTTGATAAGGATCCTTTCTTTGTACCAAAAACGGATGAGGAAAAAGAGGAGTTTGGTGATGGTTCTAGTGTGCCTCCAAATACAGCAAGAGAACTGATAAATGCTGTAAGACGGCAGAAGGGTCTTCCAGTGGAGGAAAAAGTAGTGCAGCACGCGACAAAGCAGAGGACCTTGGCTCGTAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

955

Amino Acids

105.45

Weight (kDa)

5.39

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 2 - 150 3.4e-14 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 364 - 439 2e-10 Elongation factor Tu domain 2
EF-G_D2 PF22042 364 - 439 6.5e-10 Elongation factor G domain 2
EFG_III PF14492 457 - 519 1.3e-06 Elongation Factor G, domain III
EFL1 PF25118 575 - 676 5.9e-09 Elongation factor-like GTPase 1-like domain
EFG_C PF00679 813 - 898 4e-13 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 41
AccB1I GGYRCC 3 cut(s) 146, 317, 331
AccB7I CCANNNNNTGG 1 cut(s) 1028
AccI GTMKAC 1 cut(s) 132
AccII CGCG 4 cut(s) 33, 119, 2076, 2834
AclI AACGTT 1 cut(s) 701
AclWI GGATC 8 cut(s) 118, 161, 1112, 1157, 1394, 1991, 2684, 2697
AcoI YGGCCR 1 cut(s) 336
AcsI RAATTY 3 cut(s) 482, 1137, 1231
AcuI CTGAAG 8 cut(s) 64, 693, 984, 1739, 2148, 2189, 2196, 2211
AcyI GRCGYC 2 cut(s) 135, 147
AdeI CACNNNGTG 1 cut(s) 171
AfaI GTAC 6 cut(s) 496, 641, 909, 1518, 2456, 2703
AfiI CCNNNNNNNGG 6 cut(s) 102, 404, 1028, 1172, 1173, 2273
AflIII ACRYGT 1 cut(s) 1492
AjnI CCWGG 5 cut(s) 101, 149, 642, 1126, 1359
AjuI GAANNNNNNNTTGG 2 cut(s) 2090, 2122
AloI GAACNNNNNNTCC 4 cut(s) 1479, 1511, 1628, 1660
Alw21I GWGCWC 4 cut(s) 11, 387, 2070, 2653
Alw26I GTCTC 4 cut(s) 94, 1598, 1802, 1919
AlwI GGATC 8 cut(s) 118, 161, 1112, 1157, 1394, 1991, 2684, 2697
AlwNI CAGNNNCTG 1 cut(s) 2246
Ama87I CYCGRG 1 cut(s) 723
ApoI RAATTY 3 cut(s) 482, 1137, 1231
Asp700I GAANNNNTTC 1 cut(s) 1494
AspLEI GCGC 5 cut(s) 119, 149, 344, 1255, 2202
AspS9I GGNCC 9 cut(s) 64, 524, 597, 1225, 1714, 1997, 2012, 2249, 2847
AsuC2I CCSGG 1 cut(s) 63
AsuII TTCGAA 1 cut(s) 989
AvaI CYCGRG 1 cut(s) 723
AvaII GGWCC 3 cut(s) 1714, 2249, 2847
BaeGI GKGCMC 1 cut(s) 1993
BamHI GGATCC 1 cut(s) 2689
BanI GGYRCC 3 cut(s) 146, 317, 331
BanII GRGCYC 3 cut(s) 11, 224, 387
BarI GAAGNNNNNNTAC 2 cut(s) 2406, 2438
BbsI GAAGAC 1 cut(s) 2795
Bbv12I GWGCWC 4 cut(s) 11, 387, 2070, 2653
BccI CCATC 9 cut(s) 23, 428, 673, 884, 1031, 1216, 1402, 1882, 2732
BceAI ACGGC 7 cut(s) 122, 130, 154, 1451, 2378, 2453, 2807
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BciT130I CCWGG 5 cut(s) 103, 151, 644, 1128, 1361
BciVI GTATCC 1 cut(s) 2044
BclI TGATCA 2 cut(s) 180, 2469
BcnI CCSGG 1 cut(s) 63
BcoDI GTCTC 4 cut(s) 94, 1598, 1802, 1919
BfaI CTAG 7 cut(s) 128, 957, 1482, 1769, 1917, 2637, 2745
BfmI CTRYAG 4 cut(s) 1108, 1404, 1506, 1845
BfoI RGCGCY 3 cut(s) 150, 345, 2203
BfuI GTATCC 1 cut(s) 2044
BlpI GCTNAGC 2 cut(s) 107, 2663
BmcAI AGTACT 1 cut(s) 496
Bme1390I CCNGG 6 cut(s) 63, 103, 151, 644, 1128, 1361
Bme18I GGWCC 3 cut(s) 1714, 2249, 2847
BmeT110I CYCGRG 1 cut(s) 723
BmgT120I GGNCC 9 cut(s) 64, 524, 597, 1225, 1714, 1997, 2012, 2249, 2847
BmiI GGNNCC 6 cut(s) 148, 319, 333, 525, 2080, 2691
BmrFI CCNGG 6 cut(s) 63, 103, 151, 644, 1128, 1361
BmrI ACTGGG 1 cut(s) 2680
BmsI GCATC 6 cut(s) 220, 889, 1520, 1843, 2104, 2392
BmuI ACTGGG 1 cut(s) 2680
BpiI GAAGAC 1 cut(s) 2795
BpmI CTGGAG 2 cut(s) 1330, 2016
Bpu10I CCTNAGC 1 cut(s) 2355
Bpu1102I GCTNAGC 2 cut(s) 107, 2663
Bpu14I TTCGAA 1 cut(s) 989
BpuEI CTTGAG 2 cut(s) 641, 1652
BpuMI CCSGG 1 cut(s) 63
Bsa29I ATCGAT 1 cut(s) 192
BsaBI GATNNNNATC 2 cut(s) 1857, 2688
BsaHI GRCGYC 2 cut(s) 135, 147
BsaI GGTCTC 2 cut(s) 1598, 1802
BsaXI ACNNNNNCTCC 6 cut(s) 374, 404, 879, 909, 1479, 1509
Bsc4I CCNNNNNNNGG 6 cut(s) 102, 404, 1028, 1172, 1173, 2273
Bse118I RCCGGY 3 cut(s) 334, 625, 2587
Bse1I ACTGG 5 cut(s) 1347, 1656, 2238, 2675, 2807
Bse3DI GCAATG 3 cut(s) 415, 503, 2131
Bse8I GATNNNNATC 2 cut(s) 1857, 2688
BseBI CCWGG 5 cut(s) 103, 151, 644, 1128, 1361
BseCI ATCGAT 1 cut(s) 192
BseJI GATNNNNATC 2 cut(s) 1857, 2688
BseLI CCNNNNNNNGG 6 cut(s) 102, 404, 1028, 1172, 1173, 2273
BseMI GCAATG 3 cut(s) 415, 503, 2131
BseMII CTCAG 7 cut(s) 121, 456, 1572, 1935, 2085, 2333, 2346
BseNI ACTGG 5 cut(s) 1347, 1656, 2238, 2675, 2807
BseRI GAGGAG 7 cut(s) 377, 395, 680, 1500, 2050, 2071, 2741
BseSI GKGCMC 1 cut(s) 1993
BseYI CCCAGC 1 cut(s) 211
BsgI GTGCAG 1 cut(s) 2846
Bsh1236I CGCG 4 cut(s) 33, 119, 2076, 2834
BshNI GGYRCC 3 cut(s) 146, 317, 331
BshVI ATCGAT 1 cut(s) 192
BsiHKAI GWGCWC 4 cut(s) 11, 387, 2070, 2653
BsiHKCI CYCGRG 1 cut(s) 723
BsiSI CCGG 4 cut(s) 62, 335, 626, 2588
BslFI GGGAC 1 cut(s) 576
BslI CCNNNNNNNGG 6 cut(s) 102, 404, 1028, 1172, 1173, 2273
BsmAI GTCTC 4 cut(s) 94, 1598, 1802, 1919
BsmFI GGGAC 1 cut(s) 576
Bso31I GGTCTC 2 cut(s) 1598, 1802
BsoBI CYCGRG 1 cut(s) 723
Bsp119I TTCGAA 1 cut(s) 989
Bsp1286I GDGCHC 6 cut(s) 11, 224, 387, 1993, 2070, 2653
Bsp1720I GCTNAGC 2 cut(s) 107, 2663
BspCNI CTCAG 7 cut(s) 120, 457, 1573, 1936, 2086, 2332, 2347
BspDI ATCGAT 1 cut(s) 192
BspFNI CGCG 4 cut(s) 33, 119, 2076, 2834
BspHI TCATGA 2 cut(s) 259, 1218
BspLI GGNNCC 6 cut(s) 148, 319, 333, 525, 2080, 2691
BspMAI CTGCAG 2 cut(s) 1408, 1510
BspPI GGATC 8 cut(s) 118, 161, 1112, 1157, 1394, 1991, 2684, 2697
BspQI GCTCTTC 1 cut(s) 2658
BspT104I TTCGAA 1 cut(s) 989
BspT107I GGYRCC 3 cut(s) 146, 317, 331
BspTNI GGTCTC 2 cut(s) 1598, 1802
BsrDI GCAATG 3 cut(s) 415, 503, 2131
BsrFI RCCGGY 3 cut(s) 334, 625, 2587
BsrI ACTGG 5 cut(s) 1347, 1656, 2238, 2675, 2807
BssAI RCCGGY 3 cut(s) 334, 625, 2587
BssNI GRCGYC 2 cut(s) 135, 147
BssT1I CCWWGG 6 cut(s) 527, 1285, 1740, 1780, 2000, 2850
Bst2UI CCWGG 5 cut(s) 103, 151, 644, 1128, 1361
Bst4CI ACNGT 5 cut(s) 49, 843, 1334, 1474, 1846
Bst6I CTCTTC 1 cut(s) 2658
BstACI GRCGYC 2 cut(s) 135, 147
BstAPI GCANNNNNTGC 1 cut(s) 2186
BstBI TTCGAA 1 cut(s) 989
BstC8I GCNNGC 5 cut(s) 336, 340, 1257, 1840, 2832
BstDSI CCRYGG 3 cut(s) 138, 2074, 2435
BstEII GGTNACC 1 cut(s) 737
BstFNI CGCG 4 cut(s) 33, 119, 2076, 2834
BstH2I RGCGCY 3 cut(s) 150, 345, 2203
BstHHI GCGC 5 cut(s) 119, 149, 344, 1255, 2202
BstMAI GTCTC 4 cut(s) 94, 1598, 1802, 1919
BstMWI GCNNNNNNNGC 8 cut(s) 784, 1252, 2186, 2208, 2243, 2356, 2642, 2648
BstNI CCWGG 5 cut(s) 103, 151, 644, 1128, 1361
BstNSI RCATGY 3 cut(s) 1244, 1496, 2579
BstPI GGTNACC 1 cut(s) 737
BstSCI CCNGG 6 cut(s) 61, 101, 149, 642, 1126, 1359
BstSFI CTRYAG 4 cut(s) 1108, 1404, 1506, 1845
BstSLI GKGCMC 1 cut(s) 1993
BstUI CGCG 4 cut(s) 33, 119, 2076, 2834
BstV2I GAAGAC 1 cut(s) 2795
BstX2I RGATCY 3 cut(s) 1104, 1983, 2689
BstYI RGATCY 3 cut(s) 1104, 1983, 2689
Bsu15I ATCGAT 1 cut(s) 192
BsuI GTATCC 1 cut(s) 2044
BsuTUI ATCGAT 1 cut(s) 192
BtgI CCRYGG 3 cut(s) 138, 2074, 2435
BtsIMutI CAGTG 3 cut(s) 1842, 2668, 2814
Cac8I GCNNGC 5 cut(s) 336, 340, 1257, 1840, 2832
CaiI CAGNNNCTG 1 cut(s) 2246
CciI TCATGA 2 cut(s) 259, 1218
CfoI GCGC 5 cut(s) 119, 149, 344, 1255, 2202
Cfr10I RCCGGY 3 cut(s) 334, 625, 2587
Cfr13I GGNCC 9 cut(s) 64, 524, 597, 1225, 1714, 1997, 2012, 2249, 2847
Cfr42I CCGCGG 1 cut(s) 2077
ClaI ATCGAT 1 cut(s) 192
CseI GACGC 1 cut(s) 143
Csp6I GTAC 6 cut(s) 495, 640, 908, 1517, 2455, 2702
CviQI GTAC 6 cut(s) 495, 640, 908, 1517, 2455, 2702
DinI GGCGCC 1 cut(s) 148
DraI TTTAAA 4 cut(s) 289, 1600, 1824, 2535
DraIII CACNNNGTG 1 cut(s) 171
DrdI GACNNNNNNGTC 1 cut(s) 41
DseDI GACNNNNNNGTC 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 336
Eam1104I CTCTTC 1 cut(s) 2658
EarI CTCTTC 1 cut(s) 2658
EciI GGCGGA 2 cut(s) 14, 2536
Ecl136II GAGCTC 2 cut(s) 9, 385
Eco130I CCWWGG 6 cut(s) 527, 1285, 1740, 1780, 2000, 2850
Eco147I AGGCCT 2 cut(s) 961, 1284
Eco24I GRGCYC 3 cut(s) 11, 224, 387
Eco31I GGTCTC 2 cut(s) 1598, 1802
Eco32I GATATC 1 cut(s) 1777
Eco47I GGWCC 3 cut(s) 1714, 2249, 2847
Eco53kI GAGCTC 2 cut(s) 9, 385
Eco57I CTGAAG 8 cut(s) 64, 693, 984, 1739, 2148, 2189, 2196, 2211
Eco88I CYCGRG 1 cut(s) 723
Eco91I GGTNACC 1 cut(s) 737
EcoICRI GAGCTC 2 cut(s) 9, 385
EcoO109I RGGNCCY 3 cut(s) 524, 1714, 2847
EcoO65I GGTNACC 1 cut(s) 737
EcoRII CCWGG 5 cut(s) 101, 149, 642, 1126, 1359
EcoRV GATATC 1 cut(s) 1777
EcoT14I CCWWGG 6 cut(s) 527, 1285, 1740, 1780, 2000, 2850
EcoT22I ATGCAT 2 cut(s) 505, 1535
EcoT38I GRGCYC 3 cut(s) 11, 224, 387
EgeI GGCGCC 1 cut(s) 148
EheI GGCGCC 1 cut(s) 148
ErhI CCWWGG 6 cut(s) 527, 1285, 1740, 1780, 2000, 2850
FalI AAGNNNNNCTT 4 cut(s) 1016, 1048, 1506, 1538
FaqI GGGAC 1 cut(s) 576
FauI CCCGC 1 cut(s) 571
FbaI TGATCA 2 cut(s) 180, 2469
FblI GTMKAC 1 cut(s) 132
FriOI GRGCYC 3 cut(s) 11, 224, 387
FspBI CTAG 7 cut(s) 128, 957, 1482, 1769, 1917, 2637, 2745
GlaI GCGC 5 cut(s) 118, 148, 343, 1254, 2201
GsaI CCCAGC 1 cut(s) 215
GsuI CTGGAG 2 cut(s) 1330, 2016
HaeII RGCGCY 3 cut(s) 150, 345, 2203
HapII CCGG 4 cut(s) 62, 335, 626, 2588
HgaI GACGC 1 cut(s) 143
HhaI GCGC 5 cut(s) 119, 149, 344, 1255, 2202
Hin1I GRCGYC 2 cut(s) 135, 147
Hin6I GCGC 5 cut(s) 117, 147, 342, 1253, 2200
HinP1I GCGC 5 cut(s) 117, 147, 342, 1253, 2200
HincII GTYRAC 2 cut(s) 133, 2155
HindII GTYRAC 2 cut(s) 133, 2155
HindIII AAGCTT 3 cut(s) 945, 2295, 2599
HinfI GANTC 5 cut(s) 56, 769, 953, 1178, 2057
HpaII CCGG 4 cut(s) 62, 335, 626, 2588
Hpy166II GTNNAC 6 cut(s) 133, 640, 1433, 2155, 2568, 2631
Hpy188III TCNNGA 9 cut(s) 157, 260, 725, 799, 1219, 1523, 1717, 2033, 2681
Hpy8I GTNNAC 6 cut(s) 133, 640, 1433, 2155, 2568, 2631
Hpy99I CGWCG 3 cut(s) 116, 137, 350
HpyCH4III ACNGT 5 cut(s) 49, 843, 1334, 1474, 1846
HpyCH4IV ACGT 2 cut(s) 701, 1882
HpyF10VI GCNNNNNNNGC 8 cut(s) 784, 1252, 2186, 2208, 2243, 2356, 2642, 2648
HpySE526I ACGT 2 cut(s) 701, 1882
Hsp92I GRCGYC 2 cut(s) 135, 147
HspAI GCGC 5 cut(s) 117, 147, 342, 1253, 2200
KasI GGCGCC 1 cut(s) 146
KroI GCCGGC 1 cut(s) 334
KroNI GCCGGC 1 cut(s) 336
Ksp22I TGATCA 2 cut(s) 180, 2469
KspI CCGCGG 1 cut(s) 2077
LguI GCTCTTC 1 cut(s) 2658
LmnI GCTCC 7 cut(s) 14, 382, 922, 973, 1728, 2035, 2084
LweI GCATC 6 cut(s) 220, 889, 1520, 1843, 2104, 2392
MaeI CTAG 7 cut(s) 128, 957, 1482, 1769, 1917, 2637, 2745
MaeII ACGT 2 cut(s) 701, 1882
MaeIII GTNAC 7 cut(s) 43, 737, 861, 1468, 1678, 2170, 2258
MboII GAAGA 9 cut(s) 16, 70, 562, 565, 1112, 2154, 2362, 2645, 2795
MfeI CAATTG 3 cut(s) 1386, 1863, 2486
MflI RGATCY 3 cut(s) 1104, 1983, 2689
MhlI GDGCHC 6 cut(s) 11, 224, 387, 1993, 2070, 2653
Mly113I GGCGCC 1 cut(s) 147
MlyI GAGTC 3 cut(s) 50, 778, 2051
Mph1103I ATGCAT 2 cut(s) 505, 1535
MroNI GCCGGC 1 cut(s) 334
MroXI GAANNNNTTC 1 cut(s) 1494
MslI CAYNNNNRTG 1 cut(s) 2580
MspA1I CMGCKG 3 cut(s) 2076, 2150, 2246
MspI CCGG 4 cut(s) 62, 335, 626, 2588
MspR9I CCNGG 6 cut(s) 63, 103, 151, 644, 1128, 1361
MssI GTTTAAAC 1 cut(s) 1824
MunI CAATTG 3 cut(s) 1386, 1863, 2486
MvaI CCWGG 5 cut(s) 103, 151, 644, 1128, 1361
MvnI CGCG 4 cut(s) 33, 119, 2076, 2834
MwoI GCNNNNNNNGC 8 cut(s) 784, 1252, 2186, 2208, 2243, 2356, 2642, 2648
NaeI GCCGGC 1 cut(s) 336
NarI GGCGCC 1 cut(s) 147
NciI CCSGG 1 cut(s) 63
NgoMIV GCCGGC 1 cut(s) 334
NlaIV GGNNCC 6 cut(s) 148, 319, 333, 525, 2080, 2691
NmeAIII GCCGAG 2 cut(s) 232, 574
NmuCI GTSAC 5 cut(s) 43, 737, 1468, 1678, 2258
NsiI ATGCAT 2 cut(s) 505, 1535
NspI RCATGY 3 cut(s) 1244, 1496, 2579
NspV TTCGAA 1 cut(s) 989
PagI TCATGA 2 cut(s) 259, 1218
PceI AGGCCT 2 cut(s) 961, 1284
PciI ACATGT 1 cut(s) 1492
PciSI GCTCTTC 1 cut(s) 2658
PdiI GCCGGC 1 cut(s) 336
PdmI GAANNNNTTC 1 cut(s) 1494
PfeI GAWTC 2 cut(s) 953, 1178
PflMI CCANNNNNTGG 1 cut(s) 1028
PleI GAGTC 3 cut(s) 50, 777, 2051
PluTI GGCGCC 1 cut(s) 150
PmeI GTTTAAAC 1 cut(s) 1824
PpsI GAGTC 3 cut(s) 50, 777, 2051
PpuMI RGGWCCY 2 cut(s) 1714, 2847
PscI ACATGT 1 cut(s) 1492
Psp124BI GAGCTC 2 cut(s) 11, 387
Psp1406I AACGTT 1 cut(s) 701
Psp5II RGGWCCY 2 cut(s) 1714, 2847
Psp6I CCWGG 5 cut(s) 101, 149, 642, 1126, 1359
PspEI GGTNACC 1 cut(s) 737
PspFI CCCAGC 1 cut(s) 211
PspGI CCWGG 5 cut(s) 101, 149, 642, 1126, 1359
PspN4I GGNNCC 6 cut(s) 148, 319, 333, 525, 2080, 2691
PspPI GGNCC 9 cut(s) 64, 524, 597, 1225, 1714, 1997, 2012, 2249, 2847
PspPPI RGGWCCY 2 cut(s) 1714, 2847
PstI CTGCAG 2 cut(s) 1408, 1510
PstNI CAGNNNCTG 1 cut(s) 2246
PsuI RGATCY 3 cut(s) 1104, 1983, 2689
PvuII CAGCTG 2 cut(s) 2150, 2246
RsaI GTAC 6 cut(s) 496, 641, 909, 1518, 2456, 2703
RsaNI GTAC 6 cut(s) 495, 640, 908, 1517, 2455, 2702
RseI CAYNNNNRTG 1 cut(s) 2580
SacI GAGCTC 2 cut(s) 11, 387
SacII CCGCGG 1 cut(s) 2077
SalI GTCGAC 1 cut(s) 131
SapI GCTCTTC 1 cut(s) 2658
Sau96I GGNCC 9 cut(s) 64, 524, 597, 1225, 1714, 1997, 2012, 2249, 2847
ScaI AGTACT 1 cut(s) 496
SchI GAGTC 3 cut(s) 50, 778, 2051
ScrFI CCNGG 6 cut(s) 63, 103, 151, 644, 1128, 1361
SduI GDGCHC 6 cut(s) 11, 224, 387, 1993, 2070, 2653
SfaNI GCATC 6 cut(s) 220, 889, 1520, 1843, 2104, 2392
SfcI CTRYAG 4 cut(s) 1108, 1404, 1506, 1845
SfoI GGCGCC 1 cut(s) 148
Sfr303I CCGCGG 1 cut(s) 2077
SfuI TTCGAA 1 cut(s) 989
SgrBI CCGCGG 1 cut(s) 2077
SinI GGWCC 3 cut(s) 1714, 2249, 2847
SmiMI CAYNNNNRTG 1 cut(s) 2580
SmlI CTYRAG 2 cut(s) 620, 1631
SmoI CTYRAG 2 cut(s) 620, 1631
SseBI AGGCCT 2 cut(s) 961, 1284
SspDI GGCGCC 1 cut(s) 146
SspMI CTAG 7 cut(s) 128, 957, 1482, 1769, 1917, 2637, 2745
SstI GAGCTC 2 cut(s) 11, 387
StuI AGGCCT 2 cut(s) 961, 1284
StyD4I CCNGG 6 cut(s) 61, 101, 149, 642, 1126, 1359
StyI CCWWGG 6 cut(s) 527, 1285, 1740, 1780, 2000, 2850
TaaI ACNGT 5 cut(s) 49, 843, 1334, 1474, 1846
TaiI ACGT 2 cut(s) 704, 1885
TaqI TCGA 5 cut(s) 54, 132, 156, 192, 989
TatI WGTACW 4 cut(s) 494, 907, 1516, 2454
TauI GCSGC 3 cut(s) 101, 650, 2079
TfiI GAWTC 2 cut(s) 953, 1178
TscAI CASTG 3 cut(s) 1849, 2675, 2814
TseFI GTSAC 5 cut(s) 43, 737, 1468, 1678, 2258
Tsp45I GTSAC 5 cut(s) 43, 737, 1468, 1678, 2258
TspDTI ATGAA 8 cut(s) 17, 248, 1080, 1308, 1439, 1767, 2054, 2343
TspGWI ACGGA 2 cut(s) 31, 2726
TspRI CASTG 3 cut(s) 1849, 2675, 2814
Van91I CCANNNNNTGG 1 cut(s) 1028
VpaK11BI GGWCC 3 cut(s) 1714, 2249, 2847
XapI RAATTY 3 cut(s) 482, 1137, 1231
XceI RCATGY 3 cut(s) 1244, 1496, 2579
XmiI GTMKAC 1 cut(s) 132
XmnI GAANNNNTTC 1 cut(s) 1494
XspI CTAG 7 cut(s) 128, 957, 1482, 1769, 1917, 2637, 2745
ZrmI AGTACT 1 cut(s) 496
Zsp2I ATGCAT 2 cut(s) 505, 1535
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.