Rroxscaffold_2G00121920

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0002828
Physical Location & Seq
Reverse (-)
54728704 .. 54728898
195 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00121920.1

Sequence Viewer

Length: 195 bp
ATGGCCTACCCGACCCAACAACCCCAACCCGAATCAACCACACCCGACACCCGAAAGCTCCGAAACATCTGCGTCCTAGCCCACGTCGACCACGGCAAGACGACCCTCTGCGACCACCTCATCTCCGGCTCCGACAGCGGCATCATCCACCCCAAGCTCGCCGGCCGCCTCCGCTACATGGACTCCCTCCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

64

Amino Acids

7.1

Weight (kDa)

7.86

Isoelectric Point (pI)

45.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 19 - 45 3.8e-09 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 87
AciI CCGC 3 cut(s) 138, 166, 172
AcoI YGGCCR 1 cut(s) 163
AfiI CCNNNNNNNGG 1 cut(s) 178
AjiI CACGTC 1 cut(s) 85
AluBI AGCT 2 cut(s) 58, 157
AluI AGCT 2 cut(s) 58, 157
AoxI GGCC 2 cut(s) 3, 163
BceAI ACGGC 1 cut(s) 109
BcgI CGANNNNNNTGC 2 cut(s) 51, 85
BfaI CTAG 2 cut(s) 77, 193
BisI GCNGC 2 cut(s) 139, 166
BlsI GCNGC 2 cut(s) 140, 167
BmgBI CACGTC 1 cut(s) 85
BmiI GGNNCC 1 cut(s) 130
BmsI GCATC 1 cut(s) 150
BsaJI CCNNGG 1 cut(s) 91
BsaXI ACNNNNNCTCC 3 cut(s) 107, 137, 167
Bsc4I CCNNNNNNNGG 1 cut(s) 178
Bse118I RCCGGY 1 cut(s) 161
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 1 cut(s) 144
BseLI CCNNNNNNNGG 1 cut(s) 178
BseRI GAGGAG 1 cut(s) 179
BseX3I CGGCCG 1 cut(s) 163
Bsh1285I CGRYCG 1 cut(s) 166
BshFI GGCC 2 cut(s) 5, 165
BsiEI CGRYCG 1 cut(s) 166
BsiSI CCGG 2 cut(s) 126, 162
BslI CCNNNNNNNGG 1 cut(s) 178
BsnI GGCC 2 cut(s) 5, 165
BspACI CCGC 3 cut(s) 138, 166, 172
BspANI GGCC 2 cut(s) 5, 165
BspLI GGNNCC 1 cut(s) 130
BsrFI RCCGGY 1 cut(s) 161
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 1 cut(s) 91
BstC8I GCNNGC 2 cut(s) 159, 163
BstDSI CCRYGG 1 cut(s) 91
BstF5I GGATG 1 cut(s) 144
BstMCI CGRYCG 1 cut(s) 166
BstMWI GCNNNNNNNGC 2 cut(s) 135, 171
BstZI CGGCCG 1 cut(s) 163
BsuRI GGCC 2 cut(s) 5, 165
BtgI CCRYGG 1 cut(s) 91
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 159, 163
Cfr10I RCCGGY 1 cut(s) 161
CseI GACGC 1 cut(s) 61
CviAII CATG 1 cut(s) 178
CviJI RGCY 6 cut(s) 5, 58, 80, 129, 157, 165
CviKI_1 RGCY 6 cut(s) 5, 58, 80, 129, 157, 165
EaeI YGGCCR 1 cut(s) 163
EagI CGGCCG 1 cut(s) 163
EclXI CGGCCG 1 cut(s) 163
Eco52I CGGCCG 1 cut(s) 163
FaeI CATG 1 cut(s) 181
FaiI YATR 1 cut(s) 179
FatI CATG 1 cut(s) 177
FblI GTMKAC 1 cut(s) 87
Fnu4HI GCNGC 2 cut(s) 139, 166
FokI GGATG 1 cut(s) 131
Fsp4HI GCNGC 2 cut(s) 139, 166
FspBI CTAG 2 cut(s) 77, 193
GluI GCNGC 2 cut(s) 139, 166
HaeIII GGCC 2 cut(s) 5, 165
HapII CCGG 2 cut(s) 126, 162
HgaI GACGC 1 cut(s) 61
Hin1II CATG 1 cut(s) 181
HincII GTYRAC 1 cut(s) 88
HindII GTYRAC 1 cut(s) 88
HinfI GANTC 2 cut(s) 32, 182
HpaII CCGG 2 cut(s) 126, 162
Hpy166II GTNNAC 1 cut(s) 88
Hpy188I TCNGA 2 cut(s) 62, 133
Hpy8I GTNNAC 1 cut(s) 88
Hpy99I CGWCG 1 cut(s) 89
HpyCH4IV ACGT 1 cut(s) 84
HpyF10VI GCNNNNNNNGC 2 cut(s) 135, 171
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 1 cut(s) 181
KroI GCCGGC 1 cut(s) 161
KroNI GCCGGC 1 cut(s) 163
LmnI GCTCC 2 cut(s) 63, 134
LpnPI CCDG 2 cut(s) 139, 175
LweI GCATC 1 cut(s) 150
MaeI CTAG 2 cut(s) 77, 193
MaeII ACGT 1 cut(s) 84
MlyI GAGTC 1 cut(s) 176
MmeI TCCRAC 1 cut(s) 156
MnlI CCTC 3 cut(s) 116, 128, 179
MroNI GCCGGC 1 cut(s) 161
MspA1I CMGCKG 1 cut(s) 138
MspI CCGG 2 cut(s) 126, 162
MwoI GCNNNNNNNGC 2 cut(s) 135, 171
NaeI GCCGGC 1 cut(s) 163
NgoMIV GCCGGC 1 cut(s) 161
NlaIII CATG 1 cut(s) 181
NlaIV GGNNCC 1 cut(s) 130
PdiI GCCGGC 1 cut(s) 163
PfeI GAWTC 1 cut(s) 32
PkrI GCNGC 2 cut(s) 140, 167
PleI GAGTC 1 cut(s) 176
PpsI GAGTC 1 cut(s) 176
PspN4I GGNNCC 1 cut(s) 130
SalI GTCGAC 1 cut(s) 86
SatI GCNGC 2 cut(s) 139, 166
SchI GAGTC 1 cut(s) 176
SetI ASST 4 cut(s) 60, 87, 120, 159
SfaNI GCATC 1 cut(s) 150
SsiI CCGC 3 cut(s) 138, 166, 172
SspMI CTAG 2 cut(s) 77, 193
TaiI ACGT 1 cut(s) 87
TaqI TCGA 1 cut(s) 87
TauI GCSGC 2 cut(s) 141, 168
TfiI GAWTC 1 cut(s) 32
XmiI GTMKAC 1 cut(s) 87
XspI CTAG 2 cut(s) 77, 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.