FvH4_3g14510

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0002828
Physical Location & Seq
Forward (+)
8950953 .. 8955177
4225 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g14510.t1

Sequence Viewer

Length: 3081 bp
ATGGCCGACCCGACCCAAGAACCCGATACCCGAAAGCTCCGAAACATCTGCATCCTGGCCCACGTGGACCACGGCAAGACCACCCTCTGCGACCACCTCATCTCCGGCTCCGACAGCGGCATCATCCACCCCAAGCTCGCCGGCCGCCTCCGCTACATGGACTCCCTCCTCGAGGAGCAGCGCCGCGCCATCACCATGAAAAGCTCCTCCATCGCTCTCCGCCACCGCGGCCACTCCGTCACCGTCATCGACTCCCCGGGCCACATGGACTTCTGCAGCGAAGTCTCCACCGCCGCACGCCTCAGCGACGGCGCGTTGATCCTCGTGGACGCCGTCGAGGGCGTCCACATCCAGACGCACGCCGTCCTCCGCCAGGCCTGGATCGAGAAGCTCACGCCGTGCCTGGTGATCAACAAGATCGATCGGCTCATCTCCGAGCTCGAGATGAGCCCTAGGGAGGCCTACATTAGGTTATTGAGGATTGTTCATGAGGTTAACAACATTGTTAGTGCTTTTAAATCCGTGAAGTATTTGAATGATGTGGATGCCATTCTTTCGGGGCCGGCCGGCGCCGACGACGTGGCGTTTGAGGAGATTGAGGACGATGAGGAGCTCATGTTTCAGCCACAGAAGGGCAATGTGGCTTTTGTGTGTGCTTTGGATGGTTGGGGGTTTACCATTTCGCAGTTTGCGGAGATTTATAGGGAGAAATTTGGGTGGAGTGTTAATGCATTGCAGAAGGCTCTATGGGGGCCTTGGTATTTTAATCACAAGGAGAAGAAGATTGTGGGGCAGAAGGGTGTGGCTGGTTTGAAGAAGGCTCGGCCGATGTTTGTTGAGTTTGTGCTTGAGCCGGTTTGGAGTGTGTACCAGGCGGCGTTGAAGGAGAGGGAGGAGGCTGAGGTGGTGGTTAATAAAGTGATCAAGACGTTTAAGTTGACTATACCACCGAGGGATTTGAAGGGTGATGCGAAGGGTATGGTTCAGGCTGTGATGAGTCATTGGCTTCCTCTTCATGAGGCTATCTTGTCGATGGTTATTAGGTGTATGCCTGACCCGATTGCAGCACAGTCCTATAGGATTTCACGGCTACTTCCAAAGAGAGAGGGTGTGGGTGATATGGTTGATTCTAGTGTACTTGCAGAGGCGGAGAAGGTGAGGAGATCGGTTGAAGCTTGTGATTCGAGGCCTGAGGCTCCTTGTGTTGCGTTTGTTTCGAAAATGTTTGCTGTCTCTACAAAAATGCTTCCAAAGTATGGCCTGGATGGGGAAGTTTTGGATAATACGTCTGATGAGGGTGAATTGGATGAGTGCTTCCTTGCATTTGCAAGGATTTATAGTGGGGTTCTTCGACCTGGTGAGAAAATTTATGTGCTTTCAGCTTTGTATGATCCCTCAAAAGGGGAATCGATGCAGAAGCATATTCAGGTGGCGACGTTGCAATCTCTGTATCTCATGATGGGTCAGAATTTACAGCATGTGCCAGAGGCGCAGGCAGGGGATATTGTGGCAATCCGAGGCCTTGGGCAGCAAATACTCAAAACCGCAACACTTTCATCAACAAAGAATTGCTGGCCTTTCTCCAGTATGTCATTCCAGGTTTCTCCTACTCTGAAAGTTGCAATTGAGCCATCTGATCCTGCAGACATGGGTGCATTGATGAAGGGTTTACGGCTGCTGAACAGGGCAGACCCTTTCGTAGAGGTCACGGTTTCTGCTAGAGGAGAACATGTTTTGTCTGCTGCGGGAGAAGTACATCTTGAAAGATGCATAAAAGATTTGAAAGATAGATTTGCAAGGGTAGGCTTGGAAGTGTCTAAGCCTCTTGTGTCTTTTAAAGAGACCATTTTGGGAGATGAATCCACACTTGAGAACTTAAAATCCTTTCTTGCCAGTTCGGAATATGTTGAAAAAGCAACACAAAATGGAAGATGTGTTGTTCGGGTGAAGGTCCTGAAACTCCCACCTTCTCTAACTAAGGTGATTGATGAAAGTTCCCATATTCTAGCAGATATCCTTGGAGTCAAGGTAGAAACGAGTAAAAGTTTGGACACACAGGTTGCAAGCACTGAAGAAGGTATGGATCCAATTGAAACACTAAGGAAACGTATGATGGAGGCTGTGGAGAGTGATATTTTGTCTAGTGGTGAGACTGACAAGGACCGGGCTGAGAAATGTAAAGTACAGTGGCAAAAACTTCTTAAAAGGATCTGGGCGCTTGGCCCTTGGCATATTGGCCCTAACATCCTACTTACTCCAGAGATAAAAGGAAAGGGTACTGACTCATCTGTGCTCATCCGTGGCTCCTTTCATGTTTCTGAGAAATTAGGATTTGTGGATGCTTCTGAAAATGAGAATGCAGCTGCTGAGACATCTTCAGAAGTAACCGAAGCACTGTATGCTGAAGCAGAGCGCCTTCAGAGCAGCGTTTTATCTGGGTTTCAACTGGCAACAGCAGCTGGACCTTTATGTGACGAGCCTATGTGGGGTTTGGCATTTGTGGTCGAAGCTTACATTTCACCATTACCTGCTCAGTCTGATGATTCGGAAACCTCCCACCAGCAGCCTGAGCAATATGGTATCTTCACAGGACAGGTCATGACAGCTGTTAAAGATGCGTGTAGAGCAGCTGTGCTTCAAAAGCAACCACGGCTTGTGGAAGCTATGTACTTCTGTGAGTTGATTACGCCAACTGAACAATTGGGTAATATGTATGCGGTGCTTGGACGACGGCGGACCAAAGTTTTAAAGGAAGAAATGCAGGAAGGCTCCCCATTGTTCACTGTACATGCCTATGTGCCGGTTGCTGAGAGCTTTGGTTTTGCGGATGAGCTTAGAAGATGGACTGCTGGAGCTGCAAGTGCTCTTCTTGTGCTTAGCCACTGGGAAGCACTTGATAAGGATCCTTTCTTTGTTCCAAAAACGGATGAGGAGAAAGAAGAGTTTGGCGACGGTTCTAGTGTGCCTCCAAATACTGCTAGAAAACTGATAAATGCTGTAAGACGGCAGAAGGGTCTTCCGGTGGAGGAAAAAGTAGTGCAGCACGCGACAAAGCAGAGGACCTTGGCTCGTAAAGTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

1027

Amino Acids

113.58

Weight (kDa)

5.84

Isoelectric Point (pI)

45.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 12 - 226 4.5e-44 Elongation factor Tu GTP binding domain
GTP_EFTU_D2 PF03144 440 - 518 8.3e-12 Elongation factor Tu domain 2
EF-G_D2 PF22042 440 - 518 5e-08 Elongation factor G domain 2
EFG_III PF14492 536 - 598 1.5e-06 Elongation Factor G, domain III
EFL1 PF25118 654 - 751 7.3e-09 Elongation factor-like GTPase 1-like domain
EFG_C PF00679 884 - 969 6.2e-16 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 170
Acc36I ACCTGC 1 cut(s) 2536
AccB1I GGYRCC 1 cut(s) 569
AccB7I CCANNNNNTGG 1 cut(s) 1256
AccII CGCG 4 cut(s) 186, 228, 314, 3047
AclWI GGATC 9 cut(s) 313, 389, 1385, 1631, 2078, 2091, 2216, 2897, 2910
AcoI YGGCCR 5 cut(s) 3, 142, 229, 564, 824
AcsI RAATTY 3 cut(s) 710, 1365, 1468
AcuI CTGAAG 4 cut(s) 2091, 2361, 2402, 2424
AcvI CACGTG 1 cut(s) 64
AcyI GRCGYC 3 cut(s) 330, 342, 570
AdeI CACNNNGTG 1 cut(s) 399
AfaI GTAC 7 cut(s) 869, 1137, 1755, 2184, 2278, 2669, 2787
AflIII ACRYGT 1 cut(s) 1729
AjiI CACGTC 1 cut(s) 580
AjnI CCWGG 8 cut(s) 54, 372, 377, 402, 870, 1260, 1354, 1596
AloI GAACNNNNNNTCC 2 cut(s) 1865, 1897
Alw21I GWGCWC 4 cut(s) 441, 615, 2295, 2866
Alw26I GTCTC 5 cut(s) 289, 1237, 1835, 2144, 2363
AlwI GGATC 9 cut(s) 313, 389, 1385, 1631, 2078, 2091, 2216, 2897, 2910
AlwNI CAGNNNCTG 2 cut(s) 2366, 2459
Ama87I CYCGRG 3 cut(s) 170, 256, 440
ApoI RAATTY 3 cut(s) 710, 1365, 1468
ArsI GACNNNNNNTTYG 2 cut(s) 569, 601
AspA2I CCTAGG 1 cut(s) 452
AspLEI GCGC 7 cut(s) 183, 188, 314, 572, 1492, 2218, 2415
AsuC2I CCSGG 3 cut(s) 257, 258, 2165
AsuII TTCGAA 1 cut(s) 1217
AvaI CYCGRG 3 cut(s) 170, 256, 440
AvaII GGWCC 6 cut(s) 67, 1951, 2161, 2462, 2736, 3060
AvrII CCTAGG 1 cut(s) 452
AxyI CCTNAGG 1 cut(s) 1191
BamHI GGATCC 2 cut(s) 2083, 2902
BanI GGYRCC 1 cut(s) 569
BanII GRGCYC 3 cut(s) 441, 452, 615
BauI CACGAG 1 cut(s) 323
BbrPI CACGTG 1 cut(s) 64
BbsI GAAGAC 1 cut(s) 3008
Bbv12I GWGCWC 4 cut(s) 441, 615, 2295, 2866
BbvCI CCTCAGC 2 cut(s) 302, 900
BccI CCATC 9 cut(s) 197, 218, 656, 1027, 1259, 1453, 1639, 2107, 2835
BcgI CGANNNNNNTGC 2 cut(s) 30, 64
BciT130I CCWGG 8 cut(s) 56, 374, 379, 404, 872, 1262, 1356, 1598
BclI TGATCA 2 cut(s) 408, 921
BcnI CCSGG 3 cut(s) 257, 258, 2165
BcoDI GTCTC 5 cut(s) 289, 1237, 1835, 2144, 2363
BfaI CTAG 7 cut(s) 453, 1131, 1719, 2006, 2142, 2958, 2979
BfmI CTRYAG 3 cut(s) 274, 1075, 1641
BfoI RGCGCY 4 cut(s) 184, 573, 2219, 2416
BfuAI ACCTGC 1 cut(s) 2536
BglI GCCNNNNNGGC 1 cut(s) 228
BlnI CCTAGG 1 cut(s) 452
BlpI GCTNAGC 1 cut(s) 2876
Bme18I GGWCC 6 cut(s) 67, 1951, 2161, 2462, 2736, 3060
BmeT110I CYCGRG 3 cut(s) 170, 256, 440
BmgBI CACGTC 1 cut(s) 580
BmiI GGNNCC 9 cut(s) 109, 561, 571, 753, 1197, 2085, 2305, 2770, 2904
BmrI ACTGGG 1 cut(s) 2893
BmsI GCATC 8 cut(s) 60, 129, 535, 958, 1401, 1757, 2329, 2605
BmuI ACTGGG 1 cut(s) 2893
BpiI GAAGAC 1 cut(s) 3008
BpmI CTGGAG 3 cut(s) 1567, 2241, 2871
Bpu10I CCTNAGC 3 cut(s) 302, 900, 2568
Bpu1102I GCTNAGC 1 cut(s) 2876
Bpu14I TTCGAA 1 cut(s) 1217
BpuEI CTTGAG 2 cut(s) 869, 1889
BpuMI CCSGG 3 cut(s) 257, 258, 2165
Bsa29I ATCGAT 2 cut(s) 420, 1409
BsaAI YACGTR 1 cut(s) 64
BsaBI GATNNNNATC 1 cut(s) 2901
BsaHI GRCGYC 3 cut(s) 330, 342, 570
BsaI GGTCTC 1 cut(s) 1835
BsaWI WCCGGW 1 cut(s) 3019
Bse118I RCCGGY 5 cut(s) 140, 562, 566, 853, 2800
Bse1I ACTGG 4 cut(s) 1584, 1893, 2451, 2888
Bse21I CCTNAGG 1 cut(s) 1191
Bse3DI GCAATG 2 cut(s) 643, 731
Bse8I GATNNNNATC 1 cut(s) 2901
BseBI CCWGG 8 cut(s) 56, 374, 379, 404, 872, 1262, 1356, 1598
BseCI ATCGAT 2 cut(s) 420, 1409
BseJI GATNNNNATC 1 cut(s) 2901
BseMI GCAATG 2 cut(s) 643, 731
BseMII CTCAG 9 cut(s) 316, 891, 1182, 2160, 2310, 2358, 2546, 2559, 2799
BseNI ACTGG 4 cut(s) 1584, 1893, 2451, 2888
BseRI GAGGAG 9 cut(s) 158, 188, 196, 605, 623, 908, 1174, 1737, 2945
BseX3I CGGCCG 3 cut(s) 142, 564, 824
BsgI GTGCAG 1 cut(s) 3059
Bsh1236I CGCG 4 cut(s) 186, 228, 314, 3047
Bsh1285I CGRYCG 4 cut(s) 145, 424, 567, 827
BshNI GGYRCC 1 cut(s) 569
BshVI ATCGAT 2 cut(s) 420, 1409
BsiEI CGRYCG 4 cut(s) 145, 424, 567, 827
BsiHKAI GWGCWC 4 cut(s) 441, 615, 2295, 2866
BsiHKCI CYCGRG 3 cut(s) 170, 256, 440
BsiSI CCGG 9 cut(s) 105, 141, 257, 563, 567, 854, 2164, 2801, 3020
BsmAI GTCTC 5 cut(s) 289, 1237, 1835, 2144, 2363
BsmI GAATGC 1 cut(s) 2362
Bso31I GGTCTC 1 cut(s) 1835
BsoBI CYCGRG 3 cut(s) 170, 256, 440
Bsp119I TTCGAA 1 cut(s) 1217
Bsp1286I GDGCHC 5 cut(s) 441, 452, 615, 2295, 2866
Bsp1407I TGTACA 1 cut(s) 2785
Bsp1720I GCTNAGC 1 cut(s) 2876
BspCNI CTCAG 9 cut(s) 315, 892, 1183, 2161, 2311, 2359, 2545, 2560, 2800
BspDI ATCGAT 2 cut(s) 420, 1409
BspFNI CGCG 4 cut(s) 186, 228, 314, 3047
BspHI TCATGA 4 cut(s) 487, 1015, 1455, 2598
BspLI GGNNCC 9 cut(s) 109, 561, 571, 753, 1197, 2085, 2305, 2770, 2904
BspMAI CTGCAG 2 cut(s) 278, 1645
BspMI ACCTGC 1 cut(s) 2536
BspPI GGATC 9 cut(s) 313, 389, 1385, 1631, 2078, 2091, 2216, 2897, 2910
BspQI GCTCTTC 1 cut(s) 2871
BspT104I TTCGAA 1 cut(s) 1217
BspT107I GGYRCC 1 cut(s) 569
BspTNI GGTCTC 1 cut(s) 1835
BsrDI GCAATG 2 cut(s) 643, 731
BsrFI RCCGGY 5 cut(s) 140, 562, 566, 853, 2800
BsrGI TGTACA 1 cut(s) 2785
BsrI ACTGG 4 cut(s) 1584, 1893, 2451, 2888
BssAI RCCGGY 5 cut(s) 140, 562, 566, 853, 2800
BssNI GRCGYC 3 cut(s) 330, 342, 570
BssSI CACGAG 1 cut(s) 323
BssT1I CCWWGG 6 cut(s) 452, 755, 1522, 2017, 2225, 3063
Bst2BI CACGAG 1 cut(s) 323
Bst2UI CCWGG 8 cut(s) 56, 374, 379, 404, 872, 1262, 1356, 1598
Bst4CI ACNGT 7 cut(s) 244, 1071, 1711, 2187, 2397, 2785, 2954
Bst6I CTCTTC 3 cut(s) 1017, 2871, 2934
BstACI GRCGYC 3 cut(s) 330, 342, 570
BstAPI GCANNNNNTGC 1 cut(s) 2399
BstAUI TGTACA 1 cut(s) 2785
BstBAI YACGTR 1 cut(s) 64
BstBI TTCGAA 1 cut(s) 1217
BstDSI CCRYGG 4 cut(s) 70, 226, 2299, 2648
BstENI CCTNNNNNAGG 3 cut(s) 170, 371, 466
BstFNI CGCG 4 cut(s) 186, 228, 314, 3047
BstH2I RGCGCY 4 cut(s) 184, 573, 2219, 2416
BstHHI GCGC 7 cut(s) 183, 188, 314, 572, 1492, 2218, 2415
BstMAI GTCTC 5 cut(s) 289, 1237, 1835, 2144, 2363
BstMCI CGRYCG 4 cut(s) 145, 424, 567, 827
BstNI CCWGG 8 cut(s) 56, 374, 379, 404, 872, 1262, 1356, 1598
BstNSI RCATGY 3 cut(s) 1481, 1733, 2792
BstSFI CTRYAG 3 cut(s) 274, 1075, 1641
BstUI CGCG 4 cut(s) 186, 228, 314, 3047
BstV2I GAAGAC 1 cut(s) 3008
BstX2I RGATCY 3 cut(s) 2083, 2208, 2902
BstYI RGATCY 3 cut(s) 2083, 2208, 2902
BstZI CGGCCG 3 cut(s) 142, 564, 824
Bsu15I ATCGAT 2 cut(s) 420, 1409
Bsu36I CCTNAGG 1 cut(s) 1191
BsuTUI ATCGAT 2 cut(s) 420, 1409
BtgI CCRYGG 4 cut(s) 70, 226, 2299, 2648
BtgZI GCGATG 1 cut(s) 196
BtrI CACGTC 1 cut(s) 580
BtsIMutI CAGTG 5 cut(s) 2067, 2192, 2393, 2781, 2881
BveI ACCTGC 1 cut(s) 2536
CaiI CAGNNNCTG 2 cut(s) 2366, 2459
CciI TCATGA 4 cut(s) 487, 1015, 1455, 2598
CfoI GCGC 7 cut(s) 183, 188, 314, 572, 1492, 2218, 2415
Cfr10I RCCGGY 5 cut(s) 140, 562, 566, 853, 2800
Cfr42I CCGCGG 1 cut(s) 229
Cfr9I CCCGGG 1 cut(s) 256
ClaI ATCGAT 2 cut(s) 420, 1409
CseI GACGC 3 cut(s) 331, 338, 364
CsiI ACCWGGT 1 cut(s) 1354
Csp6I GTAC 7 cut(s) 868, 1136, 1754, 2183, 2277, 2668, 2786
CviQI GTAC 7 cut(s) 868, 1136, 1754, 2183, 2277, 2668, 2786
DinI GGCGCC 1 cut(s) 571
DraI TTTAAA 3 cut(s) 517, 1837, 2748
DraIII CACNNNGTG 1 cut(s) 399
EaeI YGGCCR 5 cut(s) 3, 142, 229, 564, 824
EagI CGGCCG 3 cut(s) 142, 564, 824
Eam1104I CTCTTC 3 cut(s) 1017, 2871, 2934
EarI CTCTTC 3 cut(s) 1017, 2871, 2934
EciI GGCGGA 4 cut(s) 209, 359, 1163, 2749
Ecl136II GAGCTC 2 cut(s) 439, 613
EclXI CGGCCG 3 cut(s) 142, 564, 824
Eco130I CCWWGG 6 cut(s) 452, 755, 1522, 2017, 2225, 3063
Eco147I AGGCCT 4 cut(s) 377, 461, 1189, 1521
Eco24I GRGCYC 3 cut(s) 441, 452, 615
Eco31I GGTCTC 1 cut(s) 1835
Eco32I GATATC 1 cut(s) 2014
Eco47I GGWCC 6 cut(s) 67, 1951, 2161, 2462, 2736, 3060
Eco52I CGGCCG 3 cut(s) 142, 564, 824
Eco53kI GAGCTC 2 cut(s) 439, 613
Eco57I CTGAAG 4 cut(s) 2091, 2361, 2402, 2424
Eco72I CACGTG 1 cut(s) 64
Eco81I CCTNAGG 1 cut(s) 1191
Eco88I CYCGRG 3 cut(s) 170, 256, 440
EcoICRI GAGCTC 2 cut(s) 439, 613
EcoNI CCTNNNNNAGG 3 cut(s) 170, 371, 466
EcoO109I RGGNCCY 3 cut(s) 752, 1951, 3060
EcoRII CCWGG 8 cut(s) 54, 372, 377, 402, 870, 1260, 1354, 1596
EcoRV GATATC 1 cut(s) 2014
EcoT14I CCWWGG 6 cut(s) 452, 755, 1522, 2017, 2225, 3063
EcoT22I ATGCAT 2 cut(s) 733, 1772
EcoT38I GRGCYC 3 cut(s) 441, 452, 615
EgeI GGCGCC 1 cut(s) 571
EheI GGCGCC 1 cut(s) 571
ErhI CCWWGG 6 cut(s) 452, 755, 1522, 2017, 2225, 3063
FalI AAGNNNNNCTT 2 cut(s) 1743, 1775
FauI CCCGC 1 cut(s) 1738
FbaI TGATCA 2 cut(s) 408, 921
FriOI GRGCYC 3 cut(s) 441, 452, 615
FseI GGCCGGCC 1 cut(s) 566
FspBI CTAG 7 cut(s) 453, 1131, 1719, 2006, 2142, 2958, 2979
GlaI GCGC 7 cut(s) 182, 187, 313, 571, 1491, 2217, 2414
GsuI CTGGAG 3 cut(s) 1567, 2241, 2871
HaeII RGCGCY 4 cut(s) 184, 573, 2219, 2416
HapII CCGG 9 cut(s) 105, 141, 257, 563, 567, 854, 2164, 2801, 3020
HgaI GACGC 3 cut(s) 331, 338, 364
HhaI GCGC 7 cut(s) 183, 188, 314, 572, 1492, 2218, 2415
Hin1I GRCGYC 3 cut(s) 330, 342, 570
Hin6I GCGC 7 cut(s) 181, 186, 312, 570, 1490, 2216, 2413
HinP1I GCGC 7 cut(s) 181, 186, 312, 570, 1490, 2216, 2413
HincII GTYRAC 2 cut(s) 496, 939
HindII GTYRAC 2 cut(s) 496, 939
HindIII AAGCTT 2 cut(s) 1173, 2508
HpaI GTTAAC 1 cut(s) 496
HpaII CCGG 9 cut(s) 105, 141, 257, 563, 567, 854, 2164, 2801, 3020
Hpy99I CGWCG 7 cut(s) 311, 338, 578, 581, 1438, 2733, 2954
HpyCH4III ACNGT 7 cut(s) 244, 1071, 1711, 2187, 2397, 2785, 2954
HpyCH4IV ACGT 6 cut(s) 63, 579, 929, 1286, 1436, 2107
HpySE526I ACGT 6 cut(s) 63, 579, 929, 1286, 1436, 2107
Hsp92I GRCGYC 3 cut(s) 330, 342, 570
HspAI GCGC 7 cut(s) 181, 186, 312, 570, 1490, 2216, 2413
KasI GGCGCC 1 cut(s) 569
KroI GCCGGC 3 cut(s) 140, 562, 566
KroNI GCCGGC 3 cut(s) 142, 564, 568
Ksp22I TGATCA 2 cut(s) 408, 921
KspAI GTTAAC 1 cut(s) 496
KspI CCGCGG 1 cut(s) 229
LguI GCTCTTC 1 cut(s) 2871
LmnI GCTCC 9 cut(s) 42, 113, 175, 209, 610, 1201, 2309, 2774, 2852
LweI GCATC 8 cut(s) 60, 129, 535, 958, 1401, 1757, 2329, 2605
MabI ACCWGGT 1 cut(s) 1354
MaeI CTAG 7 cut(s) 453, 1131, 1719, 2006, 2142, 2958, 2979
MaeII ACGT 6 cut(s) 63, 579, 929, 1286, 1436, 2107
MaeIII GTNAC 4 cut(s) 238, 1705, 2383, 2471
MfeI CAATTG 3 cut(s) 1623, 2088, 2699
MflI RGATCY 3 cut(s) 2083, 2208, 2902
MhlI GDGCHC 5 cut(s) 441, 452, 615, 2295, 2866
MluCI AATT 9 cut(s) 710, 1301, 1365, 1468, 1567, 1623, 2088, 2324, 2699
Mly113I GGCGCC 1 cut(s) 570
MlyI GAGTC 5 cut(s) 155, 245, 1006, 2031, 2276
MmeI TCCRAC 1 cut(s) 135
Mph1103I ATGCAT 2 cut(s) 733, 1772
MroNI GCCGGC 3 cut(s) 140, 562, 566
MslI CAYNNNNRTG 2 cut(s) 194, 2793
MspA1I CMGCKG 6 cut(s) 117, 228, 2363, 2459, 2606, 2630
MspI CCGG 9 cut(s) 105, 141, 257, 563, 567, 854, 2164, 2801, 3020
MteI GCGCNGCGC 1 cut(s) 184
MunI CAATTG 3 cut(s) 1623, 2088, 2699
Mva1269I GAATGC 1 cut(s) 2362
MvaI CCWGG 8 cut(s) 56, 374, 379, 404, 872, 1262, 1356, 1598
MvnI CGCG 4 cut(s) 186, 228, 314, 3047
NaeI GCCGGC 3 cut(s) 142, 564, 568
NarI GGCGCC 1 cut(s) 570
NciI CCSGG 3 cut(s) 257, 258, 2165
NgoMIV GCCGGC 3 cut(s) 140, 562, 566
NlaIV GGNNCC 9 cut(s) 109, 561, 571, 753, 1197, 2085, 2305, 2770, 2904
NmeAIII GCCGAG 1 cut(s) 802
NmuCI GTSAC 3 cut(s) 238, 1705, 2471
NsiI ATGCAT 2 cut(s) 733, 1772
NspI RCATGY 3 cut(s) 1481, 1733, 2792
NspV TTCGAA 1 cut(s) 1217
PaeR7I CTCGAG 2 cut(s) 170, 440
PagI TCATGA 4 cut(s) 487, 1015, 1455, 2598
PceI AGGCCT 4 cut(s) 377, 461, 1189, 1521
PciI ACATGT 1 cut(s) 1729
PciSI GCTCTTC 1 cut(s) 2871
PcsI WCGNNNNNNNCGW 1 cut(s) 330
PctI GAATGC 1 cut(s) 2362
PdiI GCCGGC 3 cut(s) 142, 564, 568
PfeI GAWTC 5 cut(s) 1127, 1181, 1406, 1859, 2543
PflFI GACNNNGTC 1 cut(s) 332
PflMI CCANNNNNTGG 1 cut(s) 1256
Ple19I CGATCG 1 cut(s) 424
PleI GAGTC 5 cut(s) 155, 245, 1005, 2030, 2276
PluTI GGCGCC 1 cut(s) 573
PmaCI CACGTG 1 cut(s) 64
PmlI CACGTG 1 cut(s) 64
PpsI GAGTC 5 cut(s) 155, 245, 1005, 2030, 2276
Ppu21I YACGTR 1 cut(s) 64
PpuMI RGGWCCY 2 cut(s) 1951, 3060
PscI ACATGT 1 cut(s) 1729
Psp124BI GAGCTC 2 cut(s) 441, 615
Psp5II RGGWCCY 2 cut(s) 1951, 3060
Psp6I CCWGG 8 cut(s) 54, 372, 377, 402, 870, 1260, 1354, 1596
PspCI CACGTG 1 cut(s) 64
PspGI CCWGG 8 cut(s) 54, 372, 377, 402, 870, 1260, 1354, 1596
PspN4I GGNNCC 9 cut(s) 109, 561, 571, 753, 1197, 2085, 2305, 2770, 2904
PspPPI RGGWCCY 2 cut(s) 1951, 3060
PspXI VCTCGAGB 1 cut(s) 170
PstI CTGCAG 2 cut(s) 278, 1645
PstNI CAGNNNCTG 2 cut(s) 2366, 2459
PsuI RGATCY 3 cut(s) 2083, 2208, 2902
PsyI GACNNNGTC 1 cut(s) 332
PvuI CGATCG 1 cut(s) 424
PvuII CAGCTG 4 cut(s) 2363, 2459, 2606, 2630
RigI GGCCGGCC 1 cut(s) 566
RsaI GTAC 7 cut(s) 869, 1137, 1755, 2184, 2278, 2669, 2787
RsaNI GTAC 7 cut(s) 868, 1136, 1754, 2183, 2277, 2668, 2786
RseI CAYNNNNRTG 2 cut(s) 194, 2793
SacI GAGCTC 2 cut(s) 441, 615
SacII CCGCGG 1 cut(s) 229
SapI GCTCTTC 1 cut(s) 2871
SchI GAGTC 5 cut(s) 155, 245, 1006, 2031, 2276
SduI GDGCHC 5 cut(s) 441, 452, 615, 2295, 2866
SexAI ACCWGGT 1 cut(s) 1354
SfaNI GCATC 8 cut(s) 60, 129, 535, 958, 1401, 1757, 2329, 2605
SfcI CTRYAG 3 cut(s) 274, 1075, 1641
SfoI GGCGCC 1 cut(s) 571
Sfr274I CTCGAG 2 cut(s) 170, 440
Sfr303I CCGCGG 1 cut(s) 229
SfuI TTCGAA 1 cut(s) 1217
SgrBI CCGCGG 1 cut(s) 229
SinI GGWCC 6 cut(s) 67, 1951, 2161, 2462, 2736, 3060
SlaI CTCGAG 2 cut(s) 170, 440
SmaI CCCGGG 1 cut(s) 258
SmiMI CAYNNNNRTG 2 cut(s) 194, 2793
SmlI CTYRAG 4 cut(s) 170, 440, 848, 1868
SmoI CTYRAG 4 cut(s) 170, 440, 848, 1868
Sse9I AATT 9 cut(s) 710, 1301, 1365, 1468, 1567, 1623, 2088, 2324, 2699
SseBI AGGCCT 4 cut(s) 377, 461, 1189, 1521
SspDI GGCGCC 1 cut(s) 569
SspMI CTAG 7 cut(s) 453, 1131, 1719, 2006, 2142, 2958, 2979
SstI GAGCTC 2 cut(s) 441, 615
StuI AGGCCT 4 cut(s) 377, 461, 1189, 1521
StyI CCWWGG 6 cut(s) 452, 755, 1522, 2017, 2225, 3063
TaaI ACNGT 7 cut(s) 244, 1071, 1711, 2187, 2397, 2785, 2954
TaiI ACGT 6 cut(s) 66, 582, 932, 1289, 1439, 2110
TasI AATT 9 cut(s) 710, 1301, 1365, 1468, 1567, 1623, 2088, 2324, 2699
TatI WGTACW 5 cut(s) 1135, 1753, 2182, 2667, 2785
TauI GCSGC 6 cut(s) 120, 147, 186, 231, 296, 878
TfiI GAWTC 5 cut(s) 1127, 1181, 1406, 1859, 2543
TscAI CASTG 5 cut(s) 2074, 2192, 2400, 2788, 2888
TseFI GTSAC 3 cut(s) 238, 1705, 2471
Tsp45I GTSAC 3 cut(s) 238, 1705, 2471
TspDTI ATGAA 8 cut(s) 212, 476, 1004, 1545, 1676, 1872, 2004, 2300
TspGWI ACGGA 4 cut(s) 226, 511, 2288, 2939
TspMI CCCGGG 1 cut(s) 256
TspRI CASTG 5 cut(s) 2074, 2192, 2400, 2788, 2888
Tth111I GACNNNGTC 1 cut(s) 332
Van91I CCANNNNNTGG 1 cut(s) 1256
VpaK11BI GGWCC 6 cut(s) 67, 1951, 2161, 2462, 2736, 3060
XagI CCTNNNNNAGG 3 cut(s) 170, 371, 466
XapI RAATTY 3 cut(s) 710, 1365, 1468
XceI RCATGY 3 cut(s) 1481, 1733, 2792
XhoI CTCGAG 2 cut(s) 170, 440
XmaI CCCGGG 1 cut(s) 256
XmaJI CCTAGG 1 cut(s) 452
XspI CTAG 7 cut(s) 453, 1131, 1719, 2006, 2142, 2958, 2979
Zsp2I ATGCAT 2 cut(s) 733, 1772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.