pycom05g21330

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0002828
Physical Location & Seq
Reverse (-)
23977716 .. 23980699
2984 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g21330.1

Sequence Viewer

Length: 1362 bp
ATGACGCGTGAAAGTGTGAACGTTAATTTGTGCAGAGAAAAAAAAAAAAAAAAAAAAAAAAAAAGAAGGGACTTTGAAAAACCTTCACCGCAGCGCGGCCAAGCCTCGTTGTCGTCTGCTCCAAAAAAAAAAAAAAAAAAAATCCGAAAGCTTTTACTCGGTCCAAAAACTTCCGATTTCTCGGCTCTTATATTTCTATCAATGGCCGATAACACCCGAAATATCCGAAACATATGCCTACTGGCCCACGTGGATCACGGCAAAACCACGCTCGCCGGCCACCTAATCGCCGGCACCGGTTCTGGCGTAGTCCACCCGAAACTCGCCGGCCGCCTCCGGTTCATGGACTATCTCGACGAGGAGCAGAGGCGCGCCATCACCATGAAAAGCTCCTCCATTGCCCTCCACTACAAAGACCATTCCATCAACCTCATCGACTCCCCGGGCCACATGGACTTCTGCAGCGAGGTCTCCACCGCTGCCCGATTGAGCGACGGCGCTCTGTTTCTGGTCGACGCCGTCAAGGGCGTCCACATCCAGACCCACGCCGTGTTGCGCCAGGCCTGGATTGAGGAGCTGACGCCGTGCTTGGTGCTGAACAAGATCGACCGGCTGATCTCCGAGCTGAAATTGAGCCCCATGGAAGCTTACACGAGGTTAGTGCGTATTGTTCATCAGGTTAATTGGATTGTTAGTGCGTATAAGAGCGAGAAATACTTGTCCGACGTCGATGCTATACTTTCCGGGCCGGCTGTTGATGTGGGTGGAAGATACGTTTCAACCCCAGAAGGTGAATGTTGCGTTTGTGCGTGCTTTGGACGGCTGGGTTTCTGCATTAGTGAGTTTGCTGAGATTTACTCTTCGAAATTTGGGGTGAGTGCGGCTGCATTGACAAAGGCTTTGTGGGGACCGAGGGGACTCAGTGAAGAGCATATGCAGTTGGCTGATTTGAAATCTCTATATCTCATGATGGGCCAAGGATTAACACATGTGGCCTCTGCCCATGCCGGGATTCTTGTAGCAATTCAAGGCCTTGGCCAACATATACTGAAAAGTGCAACACTTTCATCGACGAAGAACTGTTGGCCTTTCTCCAGTATGGCATTTCAGATTGCCCCTACTCTAAGAGTCACAATTGAGCCAACTCATCCTGCAGATATGGGTGCACTGACTAAGTGTTTGCGGCTGTTGAACCGGGCTGACCTGTTTGTTGGGGTAACAGTTTCTGATAGGGGAGAAAATGTTCTGTCTGCTGCAGGAGAGGTACATCTTGAAAGATGCATAAAAGATTTGAAGGAGAGATTTGCAAGGGTGAGCCTGGAAGTGTCTCCACCTCATTGTGTTGAGAAAAAAGACTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

454

Amino Acids

49.95

Weight (kDa)

9.34

Isoelectric Point (pI)

34.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 74 - 226 3.9e-44 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 729
AccB1I GGYRCC 1 cut(s) 293
AccI GTMKAC 1 cut(s) 513
AccII CGCG 3 cut(s) 7, 96, 372
AciI CCGC 6 cut(s) 89, 96, 331, 477, 881, 1183
AclI AACGTT 1 cut(s) 21
AclWI GGATC 1 cut(s) 261
AcoI YGGCCR 5 cut(s) 97, 204, 277, 328, 1036
AcsI RAATTY 1 cut(s) 866
AcvI CACGTG 1 cut(s) 250
AcyI GRCGYC 4 cut(s) 516, 528, 581, 726
AdeI CACNNNGTG 1 cut(s) 550
AfaI GTAC 1 cut(s) 1266
AfiI CCNNNNNNNGG 3 cut(s) 95, 343, 1008
AflIII ACRYGT 2 cut(s) 5, 988
AgeI ACCGGT 1 cut(s) 296
AgsI TTSAA 7 cut(s) 77, 780, 952, 1028, 1192, 1274, 1294
AjnI CCWGG 3 cut(s) 558, 563, 1317
AloI GAACNNNNNNTCC 2 cut(s) 1227, 1259
AluBI AGCT 5 cut(s) 151, 390, 577, 625, 647
AluI AGCT 5 cut(s) 151, 390, 577, 625, 647
Alw21I GWGCWC 1 cut(s) 1168
Alw26I GTCTC 2 cut(s) 475, 1332
Alw44I GTGCAC 1 cut(s) 1164
AlwI GGATC 1 cut(s) 261
AlwNI CAGNNNCTG 1 cut(s) 1226
Ama87I CYCGRG 1 cut(s) 442
ApaLI GTGCAC 1 cut(s) 1164
ApeKI GCWGC 5 cut(s) 91, 462, 479, 884, 1253
ApoI RAATTY 1 cut(s) 866
ArsI GACNNNNNNTTYG 4 cut(s) 883, 915, 1162, 1194
AscI GGCGCGCC 1 cut(s) 370
AsiGI ACCGGT 1 cut(s) 296
Asp700I GAANNNNTTC 1 cut(s) 1242
AspLEI GCGC 5 cut(s) 96, 372, 374, 500, 558
AspS9I GGNCC 6 cut(s) 161, 244, 445, 746, 908, 973
AsuC2I CCSGG 5 cut(s) 443, 444, 745, 1009, 1196
AsuHPI GGTGA 5 cut(s) 78, 370, 803, 886, 1324
AsuII TTCGAA 1 cut(s) 863
AvaI CYCGRG 1 cut(s) 442
AvaII GGWCC 2 cut(s) 161, 908
BaeGI GKGCMC 1 cut(s) 1168
BalI TGGCCA 1 cut(s) 1038
BanI GGYRCC 1 cut(s) 293
BanII GRGCYC 1 cut(s) 638
BauI CACGAG 1 cut(s) 652
BbrPI CACGTG 1 cut(s) 250
Bbv12I GWGCWC 1 cut(s) 1168
BbvI GCAGC 5 cut(s) 103, 466, 474, 871, 1240
BccI CCATC 3 cut(s) 383, 431, 964
BceAI ACGGC 6 cut(s) 274, 503, 511, 533, 568, 836
BcgI CGANNNNNNTGC 6 cut(s) 216, 250, 643, 677, 713, 747
BciT130I CCWGG 3 cut(s) 560, 565, 1319
BcnI CCSGG 5 cut(s) 443, 444, 745, 1009, 1196
BcoDI GTCTC 2 cut(s) 475, 1332
BfmI CTRYAG 3 cut(s) 460, 1152, 1254
BfoI RGCGCY 1 cut(s) 501
BisI GCNGC 9 cut(s) 92, 97, 331, 463, 480, 882, 885, 1184, 1254
BlsI GCNGC 9 cut(s) 93, 98, 332, 464, 481, 883, 886, 1185, 1255
Bme1390I CCNGG 8 cut(s) 443, 444, 560, 565, 745, 1009, 1196, 1319
Bme18I GGWCC 2 cut(s) 161, 908
BmeT110I CYCGRG 1 cut(s) 442
BmgT120I GGNCC 6 cut(s) 161, 244, 445, 746, 908, 973
BmiI GGNNCC 2 cut(s) 295, 909
BmrFI CCNGG 8 cut(s) 443, 444, 560, 565, 745, 1009, 1196, 1319
BmsI GCATC 2 cut(s) 721, 1268
BplI GAGNNNNNCTC 2 cut(s) 842, 874
BpmI CTGGAG 1 cut(s) 1078
Bpu14I TTCGAA 1 cut(s) 863
BpuMI CCSGG 5 cut(s) 443, 444, 745, 1009, 1196
BsaAI YACGTR 1 cut(s) 250
BsaHI GRCGYC 4 cut(s) 516, 528, 581, 726
BsaI GGTCTC 1 cut(s) 475
BsaJI CCNNGG 6 cut(s) 441, 442, 639, 911, 976, 1033
BsaWI WCCGGW 2 cut(s) 296, 336
BsaXI ACNNNNNCTCC 2 cut(s) 1227, 1257
Bsc4I CCNNNNNNNGG 3 cut(s) 95, 343, 1008
Bse118I RCCGGY 6 cut(s) 275, 290, 296, 326, 609, 748
Bse1I ACTGG 2 cut(s) 246, 1095
Bse3DI GCAATG 1 cut(s) 396
BseBI CCWGG 3 cut(s) 560, 565, 1319
BseDI CCNNGG 6 cut(s) 441, 442, 639, 911, 976, 1033
BseGI GGATG 2 cut(s) 534, 1147
BseLI CCNNNNNNNGG 3 cut(s) 95, 343, 1008
BseMI GCAATG 1 cut(s) 396
BseMII CTCAG 2 cut(s) 840, 934
BseNI ACTGG 2 cut(s) 246, 1095
BsePI GCGCGC 1 cut(s) 370
BseRI GAGGAG 3 cut(s) 374, 382, 587
BseSI GKGCMC 1 cut(s) 1168
BseX3I CGGCCG 1 cut(s) 328
BseXI GCAGC 5 cut(s) 103, 466, 474, 871, 1240
BseYI CCCAGC 1 cut(s) 823
BsgI GTGCAG 1 cut(s) 52
Bsh1236I CGCG 3 cut(s) 7, 96, 372
Bsh1285I CGRYCG 2 cut(s) 331, 610
BshNI GGYRCC 1 cut(s) 293
BshTI ACCGGT 1 cut(s) 296
BsiEI CGRYCG 2 cut(s) 331, 610
BsiHKAI GWGCWC 1 cut(s) 1168
BsiHKCI CYCGRG 1 cut(s) 442
BslFI GGGAC 3 cut(s) 83, 921, 930
BslI CCNNNNNNNGG 3 cut(s) 95, 343, 1008
BsmAI GTCTC 2 cut(s) 475, 1332
BsmFI GGGAC 3 cut(s) 83, 921, 930
Bso31I GGTCTC 1 cut(s) 475
BsoBI CYCGRG 1 cut(s) 442
Bsp119I TTCGAA 1 cut(s) 863
Bsp1286I GDGCHC 2 cut(s) 638, 1168
Bsp143I GATC 3 cut(s) 253, 603, 615
Bsp19I CCATGG 1 cut(s) 639
BspACI CCGC 6 cut(s) 89, 96, 331, 477, 881, 1183
BspCNI CTCAG 2 cut(s) 841, 933
BspFNI CGCG 3 cut(s) 7, 96, 372
BspHI TCATGA 1 cut(s) 966
BspLI GGNNCC 2 cut(s) 295, 909
BspMAI CTGCAG 3 cut(s) 464, 1156, 1258
BspPI GGATC 1 cut(s) 261
BspQI GCTCTTC 1 cut(s) 921
BspT104I TTCGAA 1 cut(s) 863
BspT107I GGYRCC 1 cut(s) 293
BspTNI GGTCTC 1 cut(s) 475
BsrDI GCAATG 1 cut(s) 396
BsrFI RCCGGY 6 cut(s) 275, 290, 296, 326, 609, 748
BsrI ACTGG 2 cut(s) 246, 1095
BssAI RCCGGY 6 cut(s) 275, 290, 296, 326, 609, 748
BssECI CCNNGG 6 cut(s) 441, 442, 639, 911, 976, 1033
BssHII GCGCGC 1 cut(s) 370
BssMI GATC 3 cut(s) 253, 603, 615
BssNI GRCGYC 4 cut(s) 516, 528, 581, 726
BssSI CACGAG 1 cut(s) 652
BssT1I CCWWGG 3 cut(s) 639, 976, 1033
Bst2BI CACGAG 1 cut(s) 652
Bst2UI CCWGG 3 cut(s) 560, 565, 1319
Bst4CI ACNGT 2 cut(s) 1082, 1222
Bst6I CTCTTC 2 cut(s) 865, 921
BstACI GRCGYC 4 cut(s) 516, 528, 581, 726
BstBAI YACGTR 1 cut(s) 250
BstBI TTCGAA 1 cut(s) 863
BstC8I GCNNGC 7 cut(s) 273, 277, 292, 328, 372, 750, 811
BstDEI CTNAG 4 cut(s) 849, 920, 1124, 1173
BstDSI CCRYGG 1 cut(s) 639
BstF5I GGATG 2 cut(s) 534, 1147
BstFNI CGCG 3 cut(s) 7, 96, 372
BstH2I RGCGCY 1 cut(s) 501
BstHHI GCGC 5 cut(s) 96, 372, 374, 500, 558
BstKTI GATC 3 cut(s) 256, 606, 618
BstMAI GTCTC 2 cut(s) 475, 1332
BstMBI GATC 3 cut(s) 253, 603, 615
BstMCI CGRYCG 2 cut(s) 331, 610
BstNI CCWGG 3 cut(s) 560, 565, 1319
BstNSI RCATGY 1 cut(s) 992
BstSCI CCNGG 8 cut(s) 441, 442, 558, 563, 743, 1007, 1194, 1317
BstSFI CTRYAG 3 cut(s) 460, 1152, 1254
BstSLI GKGCMC 1 cut(s) 1168
BstUI CGCG 3 cut(s) 7, 96, 372
BstV1I GCAGC 5 cut(s) 103, 466, 474, 871, 1240
BstZI CGGCCG 1 cut(s) 328
BtgI CCRYGG 1 cut(s) 639
BtsCI GGATG 2 cut(s) 534, 1147
BtsIMutI CAGTG 2 cut(s) 928, 1166
Cac8I GCNNGC 7 cut(s) 273, 277, 292, 328, 372, 750, 811
CaiI CAGNNNCTG 1 cut(s) 1226
CciI TCATGA 1 cut(s) 966
CfoI GCGC 5 cut(s) 96, 372, 374, 500, 558
Cfr10I RCCGGY 6 cut(s) 275, 290, 296, 326, 609, 748
Cfr13I GGNCC 6 cut(s) 161, 244, 445, 746, 908, 973
Cfr9I CCCGGG 1 cut(s) 442
CseI GACGC 4 cut(s) 13, 517, 524, 589
Csp6I GTAC 1 cut(s) 1265
CspAI ACCGGT 1 cut(s) 296
CviAII CATG 7 cut(s) 343, 382, 451, 640, 967, 989, 1004
CviQI GTAC 1 cut(s) 1265
DdeI CTNAG 4 cut(s) 849, 920, 1124, 1173
DpnI GATC 3 cut(s) 255, 605, 617
DpnII GATC 3 cut(s) 253, 603, 615
DraIII CACNNNGTG 1 cut(s) 550
EaeI YGGCCR 5 cut(s) 97, 204, 277, 328, 1036
EagI CGGCCG 1 cut(s) 328
Eam1104I CTCTTC 2 cut(s) 865, 921
EarI CTCTTC 2 cut(s) 865, 921
EclXI CGGCCG 1 cut(s) 328
Eco130I CCWWGG 3 cut(s) 639, 976, 1033
Eco147I AGGCCT 2 cut(s) 563, 1032
Eco24I GRGCYC 1 cut(s) 638
Eco31I GGTCTC 1 cut(s) 475
Eco47I GGWCC 2 cut(s) 161, 908
Eco52I CGGCCG 1 cut(s) 328
Eco72I CACGTG 1 cut(s) 250
Eco88I CYCGRG 1 cut(s) 442
EcoRII CCWGG 3 cut(s) 558, 563, 1317
EcoT14I CCWWGG 3 cut(s) 639, 976, 1033
EcoT22I ATGCAT 1 cut(s) 1283
EcoT38I GRGCYC 1 cut(s) 638
ErhI CCWWGG 3 cut(s) 639, 976, 1033
FaeI CATG 7 cut(s) 346, 385, 454, 643, 970, 992, 1007
FaqI GGGAC 3 cut(s) 83, 921, 930
FatI CATG 7 cut(s) 342, 381, 450, 639, 966, 988, 1003
FauNDI CATATG 2 cut(s) 233, 933
FblI GTMKAC 1 cut(s) 513
Fnu4HI GCNGC 9 cut(s) 92, 97, 331, 463, 480, 882, 885, 1184, 1254
FokI GGATG 2 cut(s) 521, 1134
FriOI GRGCYC 1 cut(s) 638
Fsp4HI GCNGC 9 cut(s) 92, 97, 331, 463, 480, 882, 885, 1184, 1254
GlaI GCGC 5 cut(s) 95, 371, 373, 499, 557
GluI GCNGC 9 cut(s) 92, 97, 331, 463, 480, 882, 885, 1184, 1254
GsaI CCCAGC 1 cut(s) 827
GsuI CTGGAG 1 cut(s) 1078
HaeII RGCGCY 1 cut(s) 501
HgaI GACGC 4 cut(s) 13, 517, 524, 589
HhaI GCGC 5 cut(s) 96, 372, 374, 500, 558
Hin1I GRCGYC 4 cut(s) 516, 528, 581, 726
Hin1II CATG 7 cut(s) 346, 385, 454, 643, 970, 992, 1007
Hin6I GCGC 5 cut(s) 94, 370, 372, 498, 556
HinP1I GCGC 5 cut(s) 94, 370, 372, 498, 556
HincII GTYRAC 1 cut(s) 514
HindII GTYRAC 1 cut(s) 514
HindIII AAGCTT 2 cut(s) 149, 645
HinfI GANTC 4 cut(s) 437, 918, 1012, 1128
HphI GGTGA 5 cut(s) 78, 370, 803, 886, 1324
Hpy166II GTNNAC 5 cut(s) 19, 313, 514, 532, 1166
Hpy188I TCNGA 7 cut(s) 146, 175, 227, 622, 724, 1110, 1228
Hpy188III TCNNGA 4 cut(s) 353, 538, 967, 1271
Hpy8I GTNNAC 5 cut(s) 19, 313, 514, 532, 1166
Hpy99I CGWCG 6 cut(s) 359, 497, 518, 728, 731, 1075
HpyAV CCTTC 4 cut(s) 60, 93, 782, 1288
HpyCH4III ACNGT 2 cut(s) 1082, 1222
HpyCH4IV ACGT 4 cut(s) 21, 249, 726, 774
HpyF3I CTNAG 4 cut(s) 849, 920, 1124, 1173
HpySE526I ACGT 4 cut(s) 21, 249, 726, 774
Hsp92I GRCGYC 4 cut(s) 516, 528, 581, 726
Hsp92II CATG 7 cut(s) 346, 385, 454, 643, 970, 992, 1007
HspAI GCGC 5 cut(s) 94, 370, 372, 498, 556
KroI GCCGGC 4 cut(s) 275, 290, 326, 748
KroNI GCCGGC 4 cut(s) 277, 292, 328, 750
Kzo9I GATC 3 cut(s) 253, 603, 615
LguI GCTCTTC 1 cut(s) 921
LmnI GCTCC 4 cut(s) 124, 361, 395, 574
Lsp1109I GCAGC 5 cut(s) 103, 466, 474, 871, 1240
LweI GCATC 2 cut(s) 721, 1268
MaeII ACGT 4 cut(s) 21, 249, 726, 774
MaeIII GTNAC 2 cut(s) 1129, 1216
MalI GATC 3 cut(s) 255, 605, 617
MboI GATC 3 cut(s) 253, 603, 615
MboII GAAGA 4 cut(s) 780, 852, 938, 1087
MfeI CAATTG 1 cut(s) 1134
MhlI GDGCHC 2 cut(s) 638, 1168
MlsI TGGCCA 1 cut(s) 1038
MluCI AATT 6 cut(s) 25, 629, 682, 866, 1023, 1134
MluI ACGCGT 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 1038
MlyI GAGTC 3 cut(s) 431, 912, 1137
MmeI TCCRAC 1 cut(s) 747
Mox20I TGGCCA 1 cut(s) 1038
Mph1103I ATGCAT 1 cut(s) 1283
MroNI GCCGGC 4 cut(s) 275, 290, 326, 748
MroXI GAANNNNTTC 1 cut(s) 1242
MscI TGGCCA 1 cut(s) 1038
MseI TTAA 3 cut(s) 24, 681, 983
MslI CAYNNNNRTG 1 cut(s) 380
Msp20I TGGCCA 1 cut(s) 1038
MspA1I CMGCKG 1 cut(s) 479
MspR9I CCNGG 8 cut(s) 443, 444, 560, 565, 745, 1009, 1196, 1319
MunI CAATTG 1 cut(s) 1134
MvaI CCWGG 3 cut(s) 560, 565, 1319
MvnI CGCG 3 cut(s) 7, 96, 372
NaeI GCCGGC 4 cut(s) 277, 292, 328, 750
NciI CCSGG 5 cut(s) 443, 444, 745, 1009, 1196
NcoI CCATGG 1 cut(s) 639
NdeI CATATG 2 cut(s) 233, 933
NdeII GATC 3 cut(s) 253, 603, 615
NgoMIV GCCGGC 4 cut(s) 275, 290, 326, 748
NlaIII CATG 7 cut(s) 346, 385, 454, 643, 970, 992, 1007
NlaIV GGNNCC 2 cut(s) 295, 909
NmeAIII GCCGAG 1 cut(s) 161
NmuCI GTSAC 1 cut(s) 1129
NsiI ATGCAT 1 cut(s) 1283
NspI RCATGY 1 cut(s) 992
NspV TTCGAA 1 cut(s) 863
PagI TCATGA 1 cut(s) 966
PalAI GGCGCGCC 1 cut(s) 370
PauI GCGCGC 1 cut(s) 370
PceI AGGCCT 2 cut(s) 563, 1032
PciI ACATGT 1 cut(s) 988
PciSI GCTCTTC 1 cut(s) 921
PdiI GCCGGC 4 cut(s) 277, 292, 328, 750
PdmI GAANNNNTTC 1 cut(s) 1242
PfeI GAWTC 1 cut(s) 1012
PflFI GACNNNGTC 1 cut(s) 518
PinAI ACCGGT 1 cut(s) 296
PkrI GCNGC 9 cut(s) 93, 98, 332, 464, 481, 883, 886, 1185, 1255
PleI GAGTC 3 cut(s) 431, 912, 1136
PmaCI CACGTG 1 cut(s) 250
PmlI CACGTG 1 cut(s) 250
PpsI GAGTC 3 cut(s) 431, 912, 1136
Ppu21I YACGTR 1 cut(s) 250
PscI ACATGT 1 cut(s) 988
Psp1406I AACGTT 1 cut(s) 21
Psp6I CCWGG 3 cut(s) 558, 563, 1317
PspCI CACGTG 1 cut(s) 250
PspFI CCCAGC 1 cut(s) 823
PspGI CCWGG 3 cut(s) 558, 563, 1317
PspN4I GGNNCC 2 cut(s) 295, 909
PspPI GGNCC 6 cut(s) 161, 244, 445, 746, 908, 973
PstI CTGCAG 3 cut(s) 464, 1156, 1258
PstNI CAGNNNCTG 1 cut(s) 1226
PsyI GACNNNGTC 1 cut(s) 518
PteI GCGCGC 1 cut(s) 370
RsaI GTAC 1 cut(s) 1266
RsaNI GTAC 1 cut(s) 1265
RseI CAYNNNNRTG 1 cut(s) 380
SalI GTCGAC 1 cut(s) 512
SapI GCTCTTC 1 cut(s) 921
SaqAI TTAA 3 cut(s) 24, 681, 983
SatI GCNGC 9 cut(s) 92, 97, 331, 463, 480, 882, 885, 1184, 1254
Sau3AI GATC 3 cut(s) 253, 603, 615
Sau96I GGNCC 6 cut(s) 161, 244, 445, 746, 908, 973
SchI GAGTC 3 cut(s) 431, 912, 1137
ScrFI CCNGG 8 cut(s) 443, 444, 560, 565, 745, 1009, 1196, 1319
SduI GDGCHC 2 cut(s) 638, 1168
SfaNI GCATC 2 cut(s) 721, 1268
SfcI CTRYAG 3 cut(s) 460, 1152, 1254
SfuI TTCGAA 1 cut(s) 863
SgsI GGCGCGCC 1 cut(s) 370
SinI GGWCC 2 cut(s) 161, 908
SmaI CCCGGG 1 cut(s) 444
SmiMI CAYNNNNRTG 1 cut(s) 380
Sse9I AATT 6 cut(s) 25, 629, 682, 866, 1023, 1134
SseBI AGGCCT 2 cut(s) 563, 1032
SsiI CCGC 6 cut(s) 89, 96, 331, 477, 881, 1183
StuI AGGCCT 2 cut(s) 563, 1032
StyD4I CCNGG 8 cut(s) 441, 442, 558, 563, 743, 1007, 1194, 1317
StyI CCWWGG 3 cut(s) 639, 976, 1033
TaaI ACNGT 2 cut(s) 1082, 1222
TaiI ACGT 4 cut(s) 24, 252, 729, 777
TaqI TCGA 7 cut(s) 354, 435, 513, 606, 729, 863, 1070
TaqII GACCGA 2 cut(s) 149, 925
TasI AATT 6 cut(s) 25, 629, 682, 866, 1023, 1134
TauI GCSGC 4 cut(s) 99, 333, 884, 1186
TfiI GAWTC 1 cut(s) 1012
Tru1I TTAA 3 cut(s) 24, 681, 983
Tru9I TTAA 3 cut(s) 24, 681, 983
TscAI CASTG 2 cut(s) 928, 1173
TseFI GTSAC 1 cut(s) 1129
TseI GCWGC 5 cut(s) 91, 462, 479, 884, 1253
Tsp45I GTSAC 1 cut(s) 1129
TspDTI ATGAA 4 cut(s) 331, 398, 662, 1056
TspMI CCCGGG 1 cut(s) 442
TspRI CASTG 2 cut(s) 928, 1173
Tth111I GACNNNGTC 1 cut(s) 518
VneI GTGCAC 1 cut(s) 1164
VpaK11BI GGWCC 2 cut(s) 161, 908
XapI RAATTY 1 cut(s) 866
XceI RCATGY 1 cut(s) 992
XmaI CCCGGG 1 cut(s) 442
XmiI GTMKAC 1 cut(s) 513
XmnI GAANNNNTTC 1 cut(s) 1242
ZraI GACGTC 1 cut(s) 727
Zsp2I ATGCAT 1 cut(s) 1283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.