FvH4_4g04790

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb4
OG0002828
Physical Location & Seq
Forward (+)
4170371 .. 4170800
430 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_4g04790.t1

Sequence Viewer

Length: 363 bp
ATGGACTCCCTCGTCGAGGAGCAGCGCCGCGCCATCACCATGAAAAGCTCCTCCATCGCTCTCCATCATCACTGCCACTCCATCACCATCATCGACTCCCCGGGCCATATTGACTTCTGCAGCGAAGTCTCCACTACTGCCAGGCTCAGTGATGGTGCGTTGATCCTAGTCGACGTCGTCGAGGGCGTACACATCCAAATGCATGCCGTCTTGCACCAGGCCTGGATCGAGAAGCTCACGCCGTGTTTGGTGATCAACAAGATCGATAGGTTGATCTCCGAGCTCGCGATGAGCCATAGCGAGCCGTTTGTTAGGTTGTTGAGGATTGTTTCTGAGGTGTTAGTGTCACATTCCGACTCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

121

Amino Acids

13.35

Weight (kDa)

5.86

Isoelectric Point (pI)

63.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 1 - 100 2.9e-27 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 11
AatII GACGTC 1 cut(s) 177
AccI GTMKAC 1 cut(s) 171
AccII CGCG 2 cut(s) 30, 287
AciI CCGC 1 cut(s) 28
AclWI GGATC 2 cut(s) 157, 233
AcyI GRCGYC 1 cut(s) 174
AdeI CACNNNGTG 1 cut(s) 243
AfaI GTAC 1 cut(s) 189
AfiI CCNNNNNNNGG 1 cut(s) 16
AjnI CCWGG 3 cut(s) 140, 216, 221
AluBI AGCT 3 cut(s) 48, 235, 283
AluI AGCT 3 cut(s) 48, 235, 283
Alw21I GWGCWC 1 cut(s) 285
Alw26I GTCTC 1 cut(s) 133
AlwI GGATC 2 cut(s) 157, 233
Ama87I CYCGRG 1 cut(s) 100
AoxI GGCC 2 cut(s) 103, 219
ApeKI GCWGC 2 cut(s) 22, 120
AspLEI GCGC 2 cut(s) 27, 32
AspS9I GGNCC 1 cut(s) 103
AsuC2I CCSGG 2 cut(s) 101, 102
AsuHPI GGTGA 3 cut(s) 28, 76, 262
AvaI CYCGRG 1 cut(s) 100
BanII GRGCYC 1 cut(s) 285
Bbv12I GWGCWC 1 cut(s) 285
BbvI GCAGC 2 cut(s) 34, 132
BccI CCATC 6 cut(s) 41, 62, 72, 89, 95, 146
BceAI ACGGC 3 cut(s) 191, 226, 289
BciT130I CCWGG 3 cut(s) 142, 218, 223
BclI TGATCA 1 cut(s) 252
BcnI CCSGG 2 cut(s) 101, 102
BcoDI GTCTC 1 cut(s) 133
BfaI CTAG 1 cut(s) 167
BfmI CTRYAG 1 cut(s) 118
BfoI RGCGCY 1 cut(s) 28
BisI GCNGC 3 cut(s) 23, 28, 121
BlsI GCNGC 3 cut(s) 24, 29, 122
Bme1390I CCNGG 5 cut(s) 101, 102, 142, 218, 223
BmeT110I CYCGRG 1 cut(s) 100
BmgT120I GGNCC 1 cut(s) 103
BmrFI CCNGG 5 cut(s) 101, 102, 142, 218, 223
BpuMI CCSGG 2 cut(s) 101, 102
Bsa29I ATCGAT 1 cut(s) 264
BsaHI GRCGYC 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 99, 100
BsaXI ACNNNNNCTCC 2 cut(s) 62, 92
Bsc4I CCNNNNNNNGG 1 cut(s) 16
BseBI CCWGG 3 cut(s) 142, 218, 223
BseCI ATCGAT 1 cut(s) 264
BseDI CCNNGG 2 cut(s) 99, 100
BseGI GGATG 1 cut(s) 192
BseLI CCNNNNNNNGG 1 cut(s) 16
BseMII CTCAG 2 cut(s) 160, 324
BseRI GAGGAG 2 cut(s) 32, 40
BseXI GCAGC 2 cut(s) 34, 132
Bsh1236I CGCG 2 cut(s) 30, 287
BshFI GGCC 2 cut(s) 105, 221
BshVI ATCGAT 1 cut(s) 264
BsiHKAI GWGCWC 1 cut(s) 285
BsiHKCI CYCGRG 1 cut(s) 100
BsiSI CCGG 1 cut(s) 101
BslI CCNNNNNNNGG 1 cut(s) 16
BsmAI GTCTC 1 cut(s) 133
BsnI GGCC 2 cut(s) 105, 221
BsoBI CYCGRG 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 285
Bsp143I GATC 5 cut(s) 162, 225, 252, 261, 273
Bsp68I TCGCGA 1 cut(s) 287
BspACI CCGC 1 cut(s) 28
BspANI GGCC 2 cut(s) 105, 221
BspCNI CTCAG 2 cut(s) 159, 325
BspDI ATCGAT 1 cut(s) 264
BspFNI CGCG 2 cut(s) 30, 287
BspMAI CTGCAG 1 cut(s) 122
BspPI GGATC 2 cut(s) 157, 233
BssECI CCNNGG 2 cut(s) 99, 100
BssMI GATC 5 cut(s) 162, 225, 252, 261, 273
BssNI GRCGYC 1 cut(s) 174
Bst2UI CCWGG 3 cut(s) 142, 218, 223
BstACI GRCGYC 1 cut(s) 174
BstC8I GCNNGC 3 cut(s) 204, 285, 302
BstDEI CTNAG 2 cut(s) 146, 333
BstENI CCTNNNNNAGG 1 cut(s) 14
BstF5I GGATG 1 cut(s) 192
BstFNI CGCG 2 cut(s) 30, 287
BstH2I RGCGCY 1 cut(s) 28
BstHHI GCGC 2 cut(s) 27, 32
BstKTI GATC 5 cut(s) 165, 228, 255, 264, 276
BstMAI GTCTC 1 cut(s) 133
BstMBI GATC 5 cut(s) 162, 225, 252, 261, 273
BstNI CCWGG 3 cut(s) 142, 218, 223
BstNSI RCATGY 1 cut(s) 206
BstSCI CCNGG 5 cut(s) 99, 100, 140, 216, 221
BstSFI CTRYAG 1 cut(s) 118
BstUI CGCG 2 cut(s) 30, 287
BstV1I GCAGC 2 cut(s) 34, 132
Bsu15I ATCGAT 1 cut(s) 264
BsuRI GGCC 2 cut(s) 105, 221
BsuTUI ATCGAT 1 cut(s) 264
BtgZI GCGATG 2 cut(s) 40, 302
BtsCI GGATG 1 cut(s) 192
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 2 cut(s) 70, 154
BtuMI TCGCGA 1 cut(s) 287
Cac8I GCNNGC 3 cut(s) 204, 285, 302
CfoI GCGC 2 cut(s) 27, 32
Cfr13I GGNCC 1 cut(s) 103
Cfr9I CCCGGG 1 cut(s) 100
ClaI ATCGAT 1 cut(s) 264
Csp6I GTAC 1 cut(s) 188
CviAII CATG 2 cut(s) 40, 203
CviJI RGCY 8 cut(s) 48, 105, 145, 221, 235, 283, 294, 304
CviKI_1 RGCY 8 cut(s) 48, 105, 145, 221, 235, 283, 294, 304
CviQI GTAC 1 cut(s) 188
DdeI CTNAG 2 cut(s) 146, 333
DpnI GATC 5 cut(s) 164, 227, 254, 263, 275
DpnII GATC 5 cut(s) 162, 225, 252, 261, 273
DraIII CACNNNGTG 1 cut(s) 243
DrdI GACNNNNNNGTC 1 cut(s) 11
DseDI GACNNNNNNGTC 1 cut(s) 11
Ecl136II GAGCTC 1 cut(s) 283
Eco147I AGGCCT 1 cut(s) 221
Eco24I GRGCYC 1 cut(s) 285
Eco53kI GAGCTC 1 cut(s) 283
Eco88I CYCGRG 1 cut(s) 100
EcoICRI GAGCTC 1 cut(s) 283
EcoNI CCTNNNNNAGG 1 cut(s) 14
EcoRII CCWGG 3 cut(s) 140, 216, 221
EcoT22I ATGCAT 1 cut(s) 204
EcoT38I GRGCYC 1 cut(s) 285
FaeI CATG 2 cut(s) 43, 206
FaiI YATR 4 cut(s) 41, 108, 204, 297
FatI CATG 2 cut(s) 39, 202
FbaI TGATCA 1 cut(s) 252
FblI GTMKAC 1 cut(s) 171
Fnu4HI GCNGC 3 cut(s) 23, 28, 121
FokI GGATG 1 cut(s) 179
FriOI GRGCYC 1 cut(s) 285
Fsp4HI GCNGC 3 cut(s) 23, 28, 121
FspBI CTAG 1 cut(s) 167
GlaI GCGC 2 cut(s) 26, 31
GluI GCNGC 3 cut(s) 23, 28, 121
HaeII RGCGCY 1 cut(s) 28
HaeIII GGCC 2 cut(s) 105, 221
HapII CCGG 1 cut(s) 101
HhaI GCGC 2 cut(s) 27, 32
Hin1I GRCGYC 1 cut(s) 174
Hin1II CATG 2 cut(s) 43, 206
Hin6I GCGC 2 cut(s) 25, 30
HinP1I GCGC 2 cut(s) 25, 30
HincII GTYRAC 1 cut(s) 172
HindII GTYRAC 1 cut(s) 172
HinfI GANTC 3 cut(s) 5, 95, 356
HpaII CCGG 1 cut(s) 101
HphI GGTGA 3 cut(s) 28, 76, 262
Hpy166II GTNNAC 2 cut(s) 172, 190
Hpy188I TCNGA 3 cut(s) 280, 334, 355
Hpy188III TCNNGA 2 cut(s) 229, 286
Hpy8I GTNNAC 2 cut(s) 172, 190
Hpy99I CGWCG 4 cut(s) 17, 176, 179, 182
HpyCH4IV ACGT 1 cut(s) 174
HpyCH4V TGCA 3 cut(s) 120, 202, 214
HpyF3I CTNAG 2 cut(s) 146, 333
HpySE526I ACGT 1 cut(s) 174
Hsp92I GRCGYC 1 cut(s) 174
Hsp92II CATG 2 cut(s) 43, 206
HspAI GCGC 2 cut(s) 25, 30
Ksp22I TGATCA 1 cut(s) 252
Kzo9I GATC 5 cut(s) 162, 225, 252, 261, 273
LmnI GCTCC 2 cut(s) 19, 53
LpnPI CCDG 7 cut(s) 114, 127, 154, 203, 208, 230, 235
Lsp1109I GCAGC 2 cut(s) 34, 132
MaeI CTAG 1 cut(s) 167
MaeII ACGT 1 cut(s) 174
MaeIII GTNAC 1 cut(s) 345
MalI GATC 5 cut(s) 164, 227, 254, 263, 275
MboI GATC 5 cut(s) 162, 225, 252, 261, 273
MhlI GDGCHC 1 cut(s) 285
MlyI GAGTC 2 cut(s) 89, 350
MnlI CCTC 6 cut(s) 10, 20, 61, 175, 315, 328
Mph1103I ATGCAT 1 cut(s) 204
MseI TTAA 1 cut(s) 361
MslI CAYNNNNRTG 2 cut(s) 38, 197
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 5 cut(s) 101, 102, 142, 218, 223
MteI GCGCNGCGC 1 cut(s) 28
MvaI CCWGG 3 cut(s) 142, 218, 223
MvnI CGCG 2 cut(s) 30, 287
NciI CCSGG 2 cut(s) 101, 102
NdeII GATC 5 cut(s) 162, 225, 252, 261, 273
NlaIII CATG 2 cut(s) 43, 206
NmuCI GTSAC 1 cut(s) 345
NruI TCGCGA 1 cut(s) 287
NsiI ATGCAT 1 cut(s) 204
NspI RCATGY 1 cut(s) 206
PaeI GCATGC 1 cut(s) 206
PceI AGGCCT 1 cut(s) 221
PcsI WCGNNNNNNNCGW 2 cut(s) 177, 183
PflFI GACNNNGTC 1 cut(s) 176
PkrI GCNGC 3 cut(s) 24, 29, 122
PleI GAGTC 2 cut(s) 89, 350
PpsI GAGTC 2 cut(s) 89, 350
Psp124BI GAGCTC 1 cut(s) 285
Psp6I CCWGG 3 cut(s) 140, 216, 221
PspGI CCWGG 3 cut(s) 140, 216, 221
PspPI GGNCC 1 cut(s) 103
PstI CTGCAG 1 cut(s) 122
PsyI GACNNNGTC 1 cut(s) 176
RruI TCGCGA 1 cut(s) 287
RsaI GTAC 1 cut(s) 189
RsaNI GTAC 1 cut(s) 188
RseI CAYNNNNRTG 2 cut(s) 38, 197
SacI GAGCTC 1 cut(s) 285
SalI GTCGAC 1 cut(s) 170
SaqAI TTAA 1 cut(s) 361
SatI GCNGC 3 cut(s) 23, 28, 121
Sau3AI GATC 5 cut(s) 162, 225, 252, 261, 273
Sau96I GGNCC 1 cut(s) 103
SchI GAGTC 2 cut(s) 89, 350
ScrFI CCNGG 5 cut(s) 101, 102, 142, 218, 223
SduI GDGCHC 1 cut(s) 285
SetI ASST 7 cut(s) 50, 177, 237, 272, 285, 317, 339
SfcI CTRYAG 1 cut(s) 118
SmaI CCCGGG 1 cut(s) 102
SmiMI CAYNNNNRTG 2 cut(s) 38, 197
SphI GCATGC 1 cut(s) 206
SseBI AGGCCT 1 cut(s) 221
SsiI CCGC 1 cut(s) 28
SspMI CTAG 1 cut(s) 167
SstI GAGCTC 1 cut(s) 285
StuI AGGCCT 1 cut(s) 221
StyD4I CCNGG 5 cut(s) 99, 100, 140, 216, 221
TaiI ACGT 1 cut(s) 177
TaqI TCGA 6 cut(s) 15, 93, 171, 180, 228, 264
TauI GCSGC 1 cut(s) 30
Tru1I TTAA 1 cut(s) 361
Tru9I TTAA 1 cut(s) 361
TscAI CASTG 2 cut(s) 77, 154
TseFI GTSAC 1 cut(s) 345
TseI GCWGC 2 cut(s) 22, 120
Tsp45I GTSAC 1 cut(s) 345
TspDTI ATGAA 1 cut(s) 56
TspMI CCCGGG 1 cut(s) 100
TspRI CASTG 2 cut(s) 77, 154
Tth111I GACNNNGTC 1 cut(s) 176
XagI CCTNNNNNAGG 1 cut(s) 14
XceI RCATGY 1 cut(s) 206
XmaI CCCGGG 1 cut(s) 100
XmiI GTMKAC 1 cut(s) 171
XspI CTAG 1 cut(s) 167
ZraI GACGTC 1 cut(s) 175
Zsp2I ATGCAT 1 cut(s) 204
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.