RchiOBHm_Chr6g0274771

Elongation factor G C-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
6
OG0002828
Physical Location & Seq
Reverse (-)
36315997 .. 36332962
16966 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ24651

Sequence Viewer

Length: 2997 bp
ATGGCCGACCCGTCCCATCAACCCCAACCTGAATCAACCACACCCGACACCCGAAAGCTCCGAAACATCTGCGTCCTGGCCCACGTCGACCACGGCAAGACGACCCTCTGCGACCACCTCATCTCCGGCTCCGACAGCGGCATCATCCACCCCAAGCTCGCCGGCCGCCTCCGCTACATGGACTCCCTCCTCGAGGAGCAGCGCCGCGCCATCACCATGAAGAGCTCCTCCATCGCCCTCCGCCACTGCGACCACTCCGTCACCGTCATCGACTCCCCGGGCCACATGGACTTCTGCAGCGAAGTCTCCACCGCCGCCAGGCTCAGCGATGGCGCGTTGATCCTAGTCGACGCCGTCGAGGGCGTCCACATCCAAACGCACGCCGTCTTGCGCCAGGCCTGGATCGAGAAGCTCACGCCGTGCTTGGTGATCAACAAGATCGATAGGTTGATCGCCGAGCTGGGAATGAGCCCTATGGATGCGTATGTAAAGTTGTTGAAGATTGTCCATGAGGTTAATAACATTGTTAGTGCTTTTAAATCTGTGAAATACTTGAATGATGTGGATGCCATTCTTTCGGGGCCGGCCGGGGCCGACGAGTTGGCGTTTGAGGAGATTGAGGATGATGAGGAGCTCATGTTTCAGCCCCAGAAGGGCAATGTGGCCTTTGTGTGTGCTTTGGATGGTTGGGGGTTTAGTATTAGGCAATTTGCCGAGTTTTATGAGGCGAAATTCGGGTGGAGTACTAATGCATTGCAGAAGGCTTTATGGGGGCCTTGGTATTTTAATCACAAGGAGAAGAAGATTGTGGGACAGAAGGGTGTGGCGGGTTTGAAAAAGGCTCGGCCGATGTTTGTTGAGTTTGTGCTTGAGCCGGTTTGGAGTGTGTACCAGGCGGCTTTGAAGGAGAAGGAGGAGGCTCAAGTGATGGTTCAGAAGGTGATTAGAACGTTTAATTTGACTATACCACCTCGGGATTTGAAGGGTGACCCAAAAGGTATGGTTCAGGCTGTGATGAGTCGGTGGCTTCCTCTGCATGAGGCTATCTTGAACATGGTTATTAGGTGTATGCCTGACCCGATTGCAGCACAGTCATTTCGGATTACAAGGTTACTTCCAAAGAGAGAGGTTGTGGGAGATGGGGTTGATGCTAATGTACTTGCAGAGGCGGAGCTTGTGAGAAGTTCCGTGGAAGCTTGTGATTCTAGGCCTGAAGCTCCTTGTGTTGTTTTTGTTTCGAAAATGTTTGCTGTGCCAACGAAAACACTTCCAAGGTATGGCTTGGATGGGGAGATTTTGGATAATACATCTGATGAAGGTGAATTGGATGAGTGCTTCCTTGCGTTTGCAAGGATCTATAGTGGGCATGTGCCAGAGGCGCAGGCAGGGGATATTGTGGCAATCCGAGGCCTTGGGCAGCATATACTGAAAACTGCAACACTTTCATCAACAAAGAACTGTTGGCCTTTCTCCAGTATGTCATTCCAGGTTTCTCCCACTCTGAAAGTTGCAATTGAGCCATCTGATCCTGCAGATATGGGTGCATTGATGAAGGGTTTACGGCTGCTGAACAGGGCAGACCCTTTTGTAGAGGTCACGGTTTCTGCTAGAGGAGAACATGTTCTGTCTGCTGCAGGAGAAGTACATCTTGAAAGATGCATAAAAGATTTGAAGGATAGATTTGCAAGGGTAGGCTTGGAAGTGTCTAAGCCTCTTGTGTCTTTTAAAGAGACCATTTTGGGAGATGAATCCACACTTGAGAACTTAAAATCCTTTGTTGCCAGTTCAGAATATGTTGAGAAAGTGACACAAAATGGAAGGTGTGTTGTTCGGGTGAAGGTCCTGAAGCTCCCACCTTCTCTAACCAAGGTGATTGATGAAAGTTCTGATATTCTAGCAGATATCCTTGGAGGCAAGGCTGCCCAGACAGGAGAGACCAATAAAAGTTTAAACACACAGGTTGCAAGCACTGTAGAGGATGCGAATCCAATTGAAACACTAAGGAAACGTATGATGGAAGCTGTGGAAAGTGATATTTTGTCTAGTGGTGAGACTGACAAAGATCGGGCTGAGAAATGTAAAGTCCAATGGCAAAAACTTCTTAAAAGGATCTGGGCACTTGGCCCTTGGCATATTGGCCCTAACATCCTACTTGCTCCAGAAATGAAAGGAAAGGGTACTGACTCCTCTGTGCTCATCCGCGGCTCCTCTCATGTTTCTGAGAAATTAGGATTGGTGGATGCTTCTGACAATGATAATGCAGCTGCTGACACATCTTCAGAAGTAACTGAAGCATTGTATGCTGAAGCAGAGCGCCTTCATAGCAGCGTTTTATCTGGGTTTCAACTGGCAACAGCAGCTGGACCTTTATGTGACGAGCCTATGTGGGGTTTAGCATTTGTGGTTGAAGCTTACATTTCACCATTAGCTGCTCAGTCTGATGAGTTAGAAACCTCCCACCAGCAGCCTGAGCAATATGGTATCTTCACAGGACAGGTAATGACAGCCATTAAGGATGCTTGTAGAGTAGCTGTGCTTCAAAAGAAACCACGGCTTGTGGAAGCTATGTACTTCTGTGAGTTGATCACGCCAACTGAACAATTGGGTAATATGTATGCTGTGCTTGGACGAAGGCGGACGAAAGTTTTAAAGGAGGAAATGCAGGAAGGCTCTGCATTGTTCACTGTACATGCCTATGTGCCGGTTTCTGAAAGCTTTGGTTTTGCGGATGAGCTTAGAAGGTGGACTGCTGGAGCTGCAAGTGCTCTTCTTGTGCTTAGCCACTGGGAAGCTCTTGATAAGGATCCTTTCTTTGTACCAAAAACGGATGAGGAAAAAGAGGAGTTTGGTGATGGTTCTAGTGTGCCTCCAAATACAGCAAGAAAACTGATAAATGCTGTAAGACGGCAGAAGGGTCTTCCAGTGGAGGAAAAAGTAGTGCAGCACGCGACAAAGCAGAGGACCTTGGCTCGTAAAGTCCCGCTGGGAGAAAGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
EFG_C EFG_II GTP_EFTU GTP_EFTU_D2

Protein Analysis

998

Amino Acids

109.9

Weight (kDa)

5.7

Isoelectric Point (pI)

42.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GTP_EFTU PF00009 19 - 233 2.7e-43 Elongation factor Tu GTP binding domain
EFG_III PF14492 499 - 561 1.4e-06 Elongation Factor G, domain III
EFL1 PF25118 617 - 718 5.9e-09 Elongation factor-like GTPase 1-like domain
EFG_C PF00679 851 - 936 4.7e-16 Elongation factor G C-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 257
AbsI CCTCGAGG 1 cut(s) 191
AccB7I CCANNNNNTGG 1 cut(s) 1277
AccI GTMKAC 2 cut(s) 87, 348
AccII CGCG 4 cut(s) 207, 335, 2202, 2948
AclI AACGTT 1 cut(s) 950
AclWI GGATC 7 cut(s) 334, 410, 1361, 1520, 2117, 2798, 2811
AcoI YGGCCR 4 cut(s) 3, 163, 585, 845
AcsI RAATTY 1 cut(s) 731
AcuI CTGAAG 5 cut(s) 1233, 1865, 2262, 2310, 2325
AcyI GRCGYC 2 cut(s) 351, 363
AdeI CACNNNGTG 1 cut(s) 420
AfaI GTAC 8 cut(s) 745, 890, 1158, 1644, 2179, 2570, 2688, 2817
AfiI CCNNNNNNNGG 6 cut(s) 178, 193, 318, 653, 1277, 2387
AflIII ACRYGT 1 cut(s) 1618
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 6 cut(s) 75, 317, 393, 398, 891, 1485
AjuI GAANNNNNNNTTGG 2 cut(s) 2216, 2248
AloI GAACNNNNNNTCC 4 cut(s) 1605, 1637, 1754, 1786
Alw21I GWGCWC 4 cut(s) 227, 636, 2196, 2767
Alw26I GTCTC 4 cut(s) 310, 1724, 1928, 2045
AlwI GGATC 7 cut(s) 334, 410, 1361, 1520, 2117, 2798, 2811
AlwNI CAGNNNCTG 2 cut(s) 2267, 2360
Ama87I CYCGRG 3 cut(s) 191, 277, 972
ApoI RAATTY 1 cut(s) 731
Asp700I GAANNNNTTC 1 cut(s) 1620
AspLEI GCGC 6 cut(s) 204, 209, 335, 393, 1381, 2316
AsuC2I CCSGG 3 cut(s) 278, 279, 589
AsuII TTCGAA 1 cut(s) 1238
AvaI CYCGRG 3 cut(s) 191, 277, 972
AvaII GGWCC 3 cut(s) 1840, 2363, 2961
BaeGI GKGCMC 1 cut(s) 2119
BamHI GGATCC 1 cut(s) 2803
BanII GRGCYC 3 cut(s) 227, 473, 636
BarI GAAGNNNNNNTAC 2 cut(s) 2520, 2552
BbsI GAAGAC 1 cut(s) 2909
Bbv12I GWGCWC 4 cut(s) 227, 636, 2196, 2767
BceAI ACGGC 7 cut(s) 109, 338, 368, 403, 1577, 2567, 2921
BcgI CGANNNNNNTGC 2 cut(s) 51, 85
BciT130I CCWGG 6 cut(s) 77, 319, 395, 400, 893, 1487
BclI TGATCA 2 cut(s) 429, 2583
BcnI CCSGG 3 cut(s) 278, 279, 589
BcoDI GTCTC 4 cut(s) 310, 1724, 1928, 2045
BfaI CTAG 6 cut(s) 344, 1206, 1608, 1895, 2043, 2859
BfmI CTRYAG 5 cut(s) 295, 1357, 1530, 1632, 1971
BfoI RGCGCY 2 cut(s) 205, 2317
BlpI GCTNAGC 2 cut(s) 323, 2777
BmcAI AGTACT 1 cut(s) 745
Bme1390I CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 589, 893, 1487
Bme18I GGWCC 3 cut(s) 1840, 2363, 2961
BmeT110I CYCGRG 3 cut(s) 191, 277, 972
BmgBI CACGTC 1 cut(s) 85
BmiI GGNNCC 6 cut(s) 130, 582, 592, 774, 2206, 2805
BmrFI CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 589, 893, 1487
BmrI ACTGGG 1 cut(s) 2794
BmsI GCATC 8 cut(s) 150, 469, 556, 1138, 1646, 1969, 2230, 2506
BmuI ACTGGG 1 cut(s) 2794
BpiI GAAGAC 1 cut(s) 2909
BpmI CTGGAG 3 cut(s) 1456, 2142, 2772
Bpu10I CCTNAGC 1 cut(s) 2469
Bpu1102I GCTNAGC 2 cut(s) 323, 2777
Bpu14I TTCGAA 1 cut(s) 1238
BpuEI CTTGAG 3 cut(s) 890, 906, 1778
BpuMI CCSGG 3 cut(s) 278, 279, 589
Bsa29I ATCGAT 1 cut(s) 441
BsaBI GATNNNNATC 2 cut(s) 1983, 2802
BsaHI GRCGYC 2 cut(s) 351, 363
BsaI GGTCTC 2 cut(s) 1724, 1928
Bsc4I CCNNNNNNNGG 6 cut(s) 178, 193, 318, 653, 1277, 2387
Bse118I RCCGGY 4 cut(s) 161, 583, 874, 2701
Bse1I ACTGG 5 cut(s) 1473, 1782, 2352, 2789, 2921
Bse3DI GCAATG 2 cut(s) 664, 752
Bse8I GATNNNNATC 2 cut(s) 1983, 2802
BseBI CCWGG 6 cut(s) 77, 319, 395, 400, 893, 1487
BseCI ATCGAT 1 cut(s) 441
BseJI GATNNNNATC 2 cut(s) 1983, 2802
BseLI CCNNNNNNNGG 6 cut(s) 178, 193, 318, 653, 1277, 2387
BseMI GCAATG 2 cut(s) 664, 752
BseMII CTCAG 5 cut(s) 337, 2061, 2211, 2447, 2460
BseNI ACTGG 5 cut(s) 1473, 1782, 2352, 2789, 2921
BseSI GKGCMC 1 cut(s) 2119
BseX3I CGGCCG 3 cut(s) 163, 585, 845
BseYI CCCAGC 2 cut(s) 460, 2983
BsgI GTGCAG 1 cut(s) 2960
Bsh1236I CGCG 4 cut(s) 207, 335, 2202, 2948
Bsh1285I CGRYCG 3 cut(s) 166, 588, 848
BshVI ATCGAT 1 cut(s) 441
BsiEI CGRYCG 3 cut(s) 166, 588, 848
BsiHKAI GWGCWC 4 cut(s) 227, 636, 2196, 2767
BsiHKCI CYCGRG 3 cut(s) 191, 277, 972
BsiSI CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2702
BslFI GGGAC 2 cut(s) 825, 2963
BslI CCNNNNNNNGG 6 cut(s) 178, 193, 318, 653, 1277, 2387
BsmAI GTCTC 4 cut(s) 310, 1724, 1928, 2045
BsmFI GGGAC 2 cut(s) 825, 2963
Bso31I GGTCTC 2 cut(s) 1724, 1928
BsoBI CYCGRG 3 cut(s) 191, 277, 972
Bsp119I TTCGAA 1 cut(s) 1238
Bsp1286I GDGCHC 6 cut(s) 227, 473, 636, 2119, 2196, 2767
Bsp1407I TGTACA 1 cut(s) 2686
Bsp1720I GCTNAGC 2 cut(s) 323, 2777
BspCNI CTCAG 5 cut(s) 336, 2062, 2212, 2446, 2461
BspDI ATCGAT 1 cut(s) 441
BspFNI CGCG 4 cut(s) 207, 335, 2202, 2948
BspLI GGNNCC 6 cut(s) 130, 582, 592, 774, 2206, 2805
BspMAI CTGCAG 3 cut(s) 299, 1534, 1636
BspPI GGATC 7 cut(s) 334, 410, 1361, 1520, 2117, 2798, 2811
BspQI GCTCTTC 2 cut(s) 215, 2772
BspT104I TTCGAA 1 cut(s) 1238
BspTNI GGTCTC 2 cut(s) 1724, 1928
BsrDI GCAATG 2 cut(s) 664, 752
BsrFI RCCGGY 4 cut(s) 161, 583, 874, 2701
BsrGI TGTACA 1 cut(s) 2686
BsrI ACTGG 5 cut(s) 1473, 1782, 2352, 2789, 2921
BssAI RCCGGY 4 cut(s) 161, 583, 874, 2701
BssNI GRCGYC 2 cut(s) 351, 363
BssT1I CCWWGG 7 cut(s) 776, 1271, 1411, 1866, 1906, 2126, 2964
Bst2UI CCWGG 6 cut(s) 77, 319, 395, 400, 893, 1487
Bst4CI ACNGT 6 cut(s) 265, 1092, 1460, 1600, 1972, 2686
Bst6I CTCTTC 2 cut(s) 215, 2772
BstACI GRCGYC 2 cut(s) 351, 363
BstAPI GCANNNNNTGC 1 cut(s) 2300
BstAUI TGTACA 1 cut(s) 2686
BstBI TTCGAA 1 cut(s) 1238
BstC8I GCNNGC 7 cut(s) 159, 163, 381, 585, 1383, 1966, 2946
BstDEI CTNAG 9 cut(s) 323, 1707, 2000, 2070, 2220, 2433, 2469, 2735, 2777
BstDSI CCRYGG 4 cut(s) 91, 1188, 2200, 2549
BstEII GGTNACC 1 cut(s) 986
BstENI CCTNNNNNAGG 1 cut(s) 191
BstFNI CGCG 4 cut(s) 207, 335, 2202, 2948
BstH2I RGCGCY 2 cut(s) 205, 2317
BstHHI GCGC 6 cut(s) 204, 209, 335, 393, 1381, 2316
BstMAI GTCTC 4 cut(s) 310, 1724, 1928, 2045
BstMCI CGRYCG 3 cut(s) 166, 588, 848
BstNI CCWGG 6 cut(s) 77, 319, 395, 400, 893, 1487
BstNSI RCATGY 3 cut(s) 1370, 1622, 2693
BstPI GGTNACC 1 cut(s) 986
BstSCI CCNGG 9 cut(s) 75, 276, 277, 317, 393, 398, 587, 891, 1485
BstSFI CTRYAG 5 cut(s) 295, 1357, 1530, 1632, 1971
BstSLI GKGCMC 1 cut(s) 2119
BstUI CGCG 4 cut(s) 207, 335, 2202, 2948
BstV2I GAAGAC 1 cut(s) 2909
BstX2I RGATCY 3 cut(s) 1353, 2109, 2803
BstYI RGATCY 3 cut(s) 1353, 2109, 2803
BstZI CGGCCG 3 cut(s) 163, 585, 845
Bsu15I ATCGAT 1 cut(s) 441
BsuTUI ATCGAT 1 cut(s) 441
BtgI CCRYGG 4 cut(s) 91, 1188, 2200, 2549
BtgZI GCGATG 2 cut(s) 217, 342
BtrI CACGTC 1 cut(s) 85
BtsI GCAGTG 1 cut(s) 244
BtsIMutI CAGTG 5 cut(s) 244, 1968, 2682, 2782, 2928
Cac8I GCNNGC 7 cut(s) 159, 163, 381, 585, 1383, 1966, 2946
CaiI CAGNNNCTG 2 cut(s) 2267, 2360
CfoI GCGC 6 cut(s) 204, 209, 335, 393, 1381, 2316
Cfr10I RCCGGY 4 cut(s) 161, 583, 874, 2701
Cfr42I CCGCGG 1 cut(s) 2203
Cfr9I CCCGGG 1 cut(s) 277
ClaI ATCGAT 1 cut(s) 441
CseI GACGC 3 cut(s) 61, 352, 359
Csp6I GTAC 8 cut(s) 744, 889, 1157, 1643, 2178, 2569, 2687, 2816
CviQI GTAC 8 cut(s) 744, 889, 1157, 1643, 2178, 2569, 2687, 2816
DdeI CTNAG 9 cut(s) 323, 1707, 2000, 2070, 2220, 2433, 2469, 2735, 2777
DraI TTTAAA 4 cut(s) 538, 1726, 1950, 2649
DraIII CACNNNGTG 1 cut(s) 420
DrdI GACNNNNNNGTC 1 cut(s) 257
DseDI GACNNNNNNGTC 1 cut(s) 257
EaeI YGGCCR 4 cut(s) 3, 163, 585, 845
EagI CGGCCG 3 cut(s) 163, 585, 845
Eam1104I CTCTTC 2 cut(s) 215, 2772
EarI CTCTTC 2 cut(s) 215, 2772
EciI GGCGGA 3 cut(s) 230, 1184, 2650
Ecl136II GAGCTC 2 cut(s) 225, 634
EclXI CGGCCG 3 cut(s) 163, 585, 845
Eco130I CCWWGG 7 cut(s) 776, 1271, 1411, 1866, 1906, 2126, 2964
Eco147I AGGCCT 3 cut(s) 398, 1210, 1410
Eco24I GRGCYC 3 cut(s) 227, 473, 636
Eco31I GGTCTC 2 cut(s) 1724, 1928
Eco32I GATATC 1 cut(s) 1903
Eco47I GGWCC 3 cut(s) 1840, 2363, 2961
Eco52I CGGCCG 3 cut(s) 163, 585, 845
Eco53kI GAGCTC 2 cut(s) 225, 634
Eco57I CTGAAG 5 cut(s) 1233, 1865, 2262, 2310, 2325
Eco88I CYCGRG 3 cut(s) 191, 277, 972
Eco91I GGTNACC 1 cut(s) 986
EcoICRI GAGCTC 2 cut(s) 225, 634
EcoNI CCTNNNNNAGG 1 cut(s) 191
EcoO109I RGGNCCY 3 cut(s) 773, 1840, 2961
EcoO65I GGTNACC 1 cut(s) 986
EcoRII CCWGG 6 cut(s) 75, 317, 393, 398, 891, 1485
EcoRV GATATC 1 cut(s) 1903
EcoT14I CCWWGG 7 cut(s) 776, 1271, 1411, 1866, 1906, 2126, 2964
EcoT22I ATGCAT 2 cut(s) 754, 1661
EcoT38I GRGCYC 3 cut(s) 227, 473, 636
ErhI CCWWGG 7 cut(s) 776, 1271, 1411, 1866, 1906, 2126, 2964
FalI AAGNNNNNCTT 2 cut(s) 1632, 1664
FaqI GGGAC 2 cut(s) 825, 2963
FauI CCCGC 2 cut(s) 820, 2988
FbaI TGATCA 2 cut(s) 429, 2583
FblI GTMKAC 2 cut(s) 87, 348
FriOI GRGCYC 3 cut(s) 227, 473, 636
FseI GGCCGGCC 1 cut(s) 587
FspBI CTAG 6 cut(s) 344, 1206, 1608, 1895, 2043, 2859
GlaI GCGC 6 cut(s) 203, 208, 334, 392, 1380, 2315
GsaI CCCAGC 2 cut(s) 464, 2987
GsuI CTGGAG 3 cut(s) 1456, 2142, 2772
HaeII RGCGCY 2 cut(s) 205, 2317
HapII CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2702
HgaI GACGC 3 cut(s) 61, 352, 359
HhaI GCGC 6 cut(s) 204, 209, 335, 393, 1381, 2316
Hin1I GRCGYC 2 cut(s) 351, 363
Hin6I GCGC 6 cut(s) 202, 207, 333, 391, 1379, 2314
HinP1I GCGC 6 cut(s) 202, 207, 333, 391, 1379, 2314
HincII GTYRAC 2 cut(s) 88, 349
HindII GTYRAC 2 cut(s) 88, 349
HindIII AAGCTT 3 cut(s) 1194, 2409, 2713
HinfI GANTC 8 cut(s) 32, 182, 272, 1018, 1202, 1748, 1984, 2183
HpaII CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2702
Hpy166II GTNNAC 7 cut(s) 88, 349, 367, 889, 1559, 2682, 2745
Hpy188III TCNNGA 7 cut(s) 406, 974, 1048, 1649, 1843, 2159, 2795
Hpy8I GTNNAC 7 cut(s) 88, 349, 367, 889, 1559, 2682, 2745
Hpy99I CGWCG 4 cut(s) 89, 353, 359, 599
HpyCH4III ACNGT 6 cut(s) 265, 1092, 1460, 1600, 1972, 2686
HpyCH4IV ACGT 3 cut(s) 84, 950, 2008
HpyF3I CTNAG 9 cut(s) 323, 1707, 2000, 2070, 2220, 2433, 2469, 2735, 2777
HpySE526I ACGT 3 cut(s) 84, 950, 2008
Hsp92I GRCGYC 2 cut(s) 351, 363
HspAI GCGC 6 cut(s) 202, 207, 333, 391, 1379, 2314
KroI GCCGGC 2 cut(s) 161, 583
KroNI GCCGGC 2 cut(s) 163, 585
Ksp22I TGATCA 2 cut(s) 429, 2583
KspI CCGCGG 1 cut(s) 2203
LguI GCTCTTC 2 cut(s) 215, 2772
LweI GCATC 8 cut(s) 150, 469, 556, 1138, 1646, 1969, 2230, 2506
MaeI CTAG 6 cut(s) 344, 1206, 1608, 1895, 2043, 2859
MaeII ACGT 3 cut(s) 84, 950, 2008
MaeIII GTNAC 7 cut(s) 259, 986, 1110, 1594, 1804, 2284, 2372
MboII GAAGA 8 cut(s) 232, 511, 811, 814, 2268, 2476, 2759, 2909
MfeI CAATTG 3 cut(s) 1512, 1989, 2600
MflI RGATCY 3 cut(s) 1353, 2109, 2803
MhlI GDGCHC 6 cut(s) 227, 473, 636, 2119, 2196, 2767
MluCI AATT 8 cut(s) 707, 731, 955, 1322, 1512, 1989, 2225, 2600
MlyI GAGTC 4 cut(s) 176, 266, 1027, 2177
MmeI TCCRAC 1 cut(s) 156
Mph1103I ATGCAT 2 cut(s) 754, 1661
MroNI GCCGGC 2 cut(s) 161, 583
MroXI GAANNNNTTC 1 cut(s) 1620
MslI CAYNNNNRTG 2 cut(s) 215, 2694
MspA1I CMGCKG 5 cut(s) 138, 2202, 2264, 2360, 2983
MspI CCGG 7 cut(s) 126, 162, 278, 584, 588, 875, 2702
MspR9I CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 589, 893, 1487
MssI GTTTAAAC 1 cut(s) 1950
MteI GCGCNGCGC 1 cut(s) 205
MunI CAATTG 3 cut(s) 1512, 1989, 2600
MvaI CCWGG 6 cut(s) 77, 319, 395, 400, 893, 1487
MvnI CGCG 4 cut(s) 207, 335, 2202, 2948
NaeI GCCGGC 2 cut(s) 163, 585
NciI CCSGG 3 cut(s) 278, 279, 589
NgoMIV GCCGGC 2 cut(s) 161, 583
NlaIV GGNNCC 6 cut(s) 130, 582, 592, 774, 2206, 2805
NmeAIII GCCGAG 3 cut(s) 481, 739, 823
NmuCI GTSAC 5 cut(s) 259, 986, 1594, 1804, 2372
NsiI ATGCAT 2 cut(s) 754, 1661
NspI RCATGY 3 cut(s) 1370, 1622, 2693
NspV TTCGAA 1 cut(s) 1238
PaeR7I CTCGAG 1 cut(s) 191
PceI AGGCCT 3 cut(s) 398, 1210, 1410
PciI ACATGT 1 cut(s) 1618
PciSI GCTCTTC 2 cut(s) 215, 2772
PcsI WCGNNNNNNNCGW 1 cut(s) 354
PdiI GCCGGC 2 cut(s) 163, 585
PdmI GAANNNNTTC 1 cut(s) 1620
PfeI GAWTC 4 cut(s) 32, 1202, 1748, 1984
PflFI GACNNNGTC 1 cut(s) 353
PflMI CCANNNNNTGG 1 cut(s) 1277
PleI GAGTC 4 cut(s) 176, 266, 1026, 2177
PmeI GTTTAAAC 1 cut(s) 1950
PpsI GAGTC 4 cut(s) 176, 266, 1026, 2177
PpuMI RGGWCCY 2 cut(s) 1840, 2961
PscI ACATGT 1 cut(s) 1618
Psp124BI GAGCTC 2 cut(s) 227, 636
Psp1406I AACGTT 1 cut(s) 950
Psp5II RGGWCCY 2 cut(s) 1840, 2961
Psp6I CCWGG 6 cut(s) 75, 317, 393, 398, 891, 1485
PspEI GGTNACC 1 cut(s) 986
PspFI CCCAGC 2 cut(s) 460, 2983
PspGI CCWGG 6 cut(s) 75, 317, 393, 398, 891, 1485
PspN4I GGNNCC 6 cut(s) 130, 582, 592, 774, 2206, 2805
PspPPI RGGWCCY 2 cut(s) 1840, 2961
PspXI VCTCGAGB 1 cut(s) 191
PstI CTGCAG 3 cut(s) 299, 1534, 1636
PstNI CAGNNNCTG 2 cut(s) 2267, 2360
PsuI RGATCY 3 cut(s) 1353, 2109, 2803
PsyI GACNNNGTC 1 cut(s) 353
PvuII CAGCTG 2 cut(s) 2264, 2360
RigI GGCCGGCC 1 cut(s) 587
RsaI GTAC 8 cut(s) 745, 890, 1158, 1644, 2179, 2570, 2688, 2817
RsaNI GTAC 8 cut(s) 744, 889, 1157, 1643, 2178, 2569, 2687, 2816
RseI CAYNNNNRTG 2 cut(s) 215, 2694
SacI GAGCTC 2 cut(s) 227, 636
SacII CCGCGG 1 cut(s) 2203
SalI GTCGAC 2 cut(s) 86, 347
SapI GCTCTTC 2 cut(s) 215, 2772
ScaI AGTACT 1 cut(s) 745
SchI GAGTC 4 cut(s) 176, 266, 1027, 2177
ScrFI CCNGG 9 cut(s) 77, 278, 279, 319, 395, 400, 589, 893, 1487
SduI GDGCHC 6 cut(s) 227, 473, 636, 2119, 2196, 2767
SfaNI GCATC 8 cut(s) 150, 469, 556, 1138, 1646, 1969, 2230, 2506
SfcI CTRYAG 5 cut(s) 295, 1357, 1530, 1632, 1971
Sfr274I CTCGAG 1 cut(s) 191
Sfr303I CCGCGG 1 cut(s) 2203
SfuI TTCGAA 1 cut(s) 1238
SgrBI CCGCGG 1 cut(s) 2203
SinI GGWCC 3 cut(s) 1840, 2363, 2961
SlaI CTCGAG 1 cut(s) 191
SmaI CCCGGG 1 cut(s) 279
SmiMI CAYNNNNRTG 2 cut(s) 215, 2694
SmlI CTYRAG 4 cut(s) 191, 869, 921, 1757
SmoI CTYRAG 4 cut(s) 191, 869, 921, 1757
Sse9I AATT 8 cut(s) 707, 731, 955, 1322, 1512, 1989, 2225, 2600
SseBI AGGCCT 3 cut(s) 398, 1210, 1410
SspMI CTAG 6 cut(s) 344, 1206, 1608, 1895, 2043, 2859
SstI GAGCTC 2 cut(s) 227, 636
StuI AGGCCT 3 cut(s) 398, 1210, 1410
StyD4I CCNGG 9 cut(s) 75, 276, 277, 317, 393, 398, 587, 891, 1485
StyI CCWWGG 7 cut(s) 776, 1271, 1411, 1866, 1906, 2126, 2964
TaaI ACNGT 6 cut(s) 265, 1092, 1460, 1600, 1972, 2686
TaiI ACGT 3 cut(s) 87, 953, 2011
TaqI TCGA 8 cut(s) 87, 192, 270, 348, 357, 405, 441, 1238
TasI AATT 8 cut(s) 707, 731, 955, 1322, 1512, 1989, 2225, 2600
TatI WGTACW 5 cut(s) 743, 1156, 1642, 2568, 2686
TauI GCSGC 6 cut(s) 141, 168, 207, 317, 899, 2205
TfiI GAWTC 4 cut(s) 32, 1202, 1748, 1984
TscAI CASTG 5 cut(s) 251, 1975, 2689, 2789, 2928
TseFI GTSAC 5 cut(s) 259, 986, 1594, 1804, 2372
Tsp45I GTSAC 5 cut(s) 259, 986, 1594, 1804, 2372
TspDTI ATGAA 8 cut(s) 233, 1329, 1434, 1565, 1761, 1893, 2180, 2309
TspGWI ACGGA 3 cut(s) 247, 1177, 2840
TspMI CCCGGG 1 cut(s) 277
TspRI CASTG 5 cut(s) 251, 1975, 2689, 2789, 2928
Tth111I GACNNNGTC 1 cut(s) 353
Van91I CCANNNNNTGG 1 cut(s) 1277
VpaK11BI GGWCC 3 cut(s) 1840, 2363, 2961
XagI CCTNNNNNAGG 1 cut(s) 191
XapI RAATTY 1 cut(s) 731
XceI RCATGY 3 cut(s) 1370, 1622, 2693
XhoI CTCGAG 1 cut(s) 191
XmaI CCCGGG 1 cut(s) 277
XmiI GTMKAC 2 cut(s) 87, 348
XmnI GAANNNNTTC 1 cut(s) 1620
XspI CTAG 6 cut(s) 344, 1206, 1608, 1895, 2043, 2859
ZrmI AGTACT 1 cut(s) 745
Zsp2I ATGCAT 2 cut(s) 754, 1661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.