AT3G27160

Ribosomal protein S21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002783
Physical Location & Seq
Forward (+)
10017321 .. 10019442
2122 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G27160.2

Sequence Viewer

Length: 552 bp
ATGGCGGCTTCGTTCTCACTCACGAGCTTCATCTCCTTCATCTCACCATTCAAATCTCAAACTAAACCTACACCACCACCAAATCTCACTCTTCCTTCTCCAACAATCTCCCAAAGGCGAAGAAATGATCTCGCTATCGAATCAATGGCGGTCGAAGAAACTTCCTCAACCGCTTCTTCACTTTCCTCTGAGCTTGCTTCCGTGATTTGTCCCTCGCTTGCTTACTCCAACACACTCTTCTTCAGTTCTGGATACAATGTGCAAGTGTTTGTTGAAGATAATGAGTCAGAGGAGAGGCTTGTGAATCGATTTAGGAGAGAAGTGATGAGAACTGGTGTTATACAGGAATGTAAGAGGAGAAGATACTTTGAGAATAAACAAGATGAGAAGAAACGTAGGACTCGTGATGCTGCTAAGCGTAATAAGAAAAGGCGTCCTCAAGCAAGGTTTACTCAAGAAACAAGAGAAGAAGCAGCAGCAGCAACTAAGAGTAAGAAAAAAGATGAGGAAGAAGACAACTGGGAGATGCCTGGTGGAGATGTACCTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

183

Amino Acids

20.91

Weight (kDa)

9.44

Isoelectric Point (pI)

89.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 88 - 141 4.2e-12 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 5, 149, 171
AcuI CTGAAG 1 cut(s) 226
AcyI GRCGYC 1 cut(s) 433
AfaI GTAC 1 cut(s) 543
AgsI TTSAA 2 cut(s) 52, 275
AjnI CCWGG 1 cut(s) 529
AjuI GAANNNNNNNTTGG 2 cut(s) 221, 253
AluBI AGCT 2 cut(s) 27, 193
AluI AGCT 2 cut(s) 27, 193
ApeKI GCWGC 4 cut(s) 410, 473, 476, 479
AsuHPI GGTGA 1 cut(s) 36
BauI CACGAG 2 cut(s) 22, 402
BbsI GAAGAC 1 cut(s) 519
BbvI GCAGC 4 cut(s) 397, 485, 488, 491
BciT130I CCWGG 1 cut(s) 531
BciVI GTATCC 1 cut(s) 245
BfuI GTATCC 1 cut(s) 245
BisI GCNGC 5 cut(s) 6, 411, 474, 477, 480
BlpI GCTNAGC 1 cut(s) 414
BlsI GCNGC 5 cut(s) 7, 412, 475, 478, 481
Bme1390I CCNGG 1 cut(s) 531
BmrFI CCNGG 1 cut(s) 531
BmrI ACTGGG 1 cut(s) 529
BmsI GCATC 2 cut(s) 397, 516
BmuI ACTGGG 1 cut(s) 529
BpiI GAAGAC 1 cut(s) 519
Bpu1102I GCTNAGC 1 cut(s) 414
BpuEI CTTGAG 2 cut(s) 423, 438
Bsa29I ATCGAT 1 cut(s) 307
BsaHI GRCGYC 1 cut(s) 433
Bse1I ACTGG 2 cut(s) 337, 524
BseBI CCWGG 1 cut(s) 531
BseCI ATCGAT 1 cut(s) 307
BseMII CTCAG 1 cut(s) 180
BseNI ACTGG 2 cut(s) 337, 524
BseRI GAGGAG 2 cut(s) 305, 370
BseXI GCAGC 4 cut(s) 397, 485, 488, 491
Bsh1285I CGRYCG 1 cut(s) 153
BshVI ATCGAT 1 cut(s) 307
BsiEI CGRYCG 1 cut(s) 153
BslFI GGGAC 1 cut(s) 195
BsmFI GGGAC 1 cut(s) 195
Bsp143I GATC 1 cut(s) 127
Bsp1720I GCTNAGC 1 cut(s) 414
BspACI CCGC 3 cut(s) 5, 149, 171
BspCNI CTCAG 1 cut(s) 181
BspDI ATCGAT 1 cut(s) 307
BsrI ACTGG 2 cut(s) 337, 524
BssMI GATC 1 cut(s) 127
BssNI GRCGYC 1 cut(s) 433
BssSI CACGAG 2 cut(s) 22, 402
Bst2BI CACGAG 2 cut(s) 22, 402
Bst2UI CCWGG 1 cut(s) 531
Bst6I CTCTTC 2 cut(s) 96, 242
BstACI GRCGYC 1 cut(s) 433
BstC8I GCNNGC 2 cut(s) 195, 219
BstDEI CTNAG 3 cut(s) 189, 414, 486
BstKTI GATC 1 cut(s) 130
BstMBI GATC 1 cut(s) 127
BstMCI CGRYCG 1 cut(s) 153
BstMWI GCNNNNNNNGC 1 cut(s) 479
BstNI CCWGG 1 cut(s) 531
BstSCI CCNGG 1 cut(s) 529
BstV1I GCAGC 4 cut(s) 397, 485, 488, 491
BstV2I GAAGAC 1 cut(s) 519
Bsu15I ATCGAT 1 cut(s) 307
BsuI GTATCC 1 cut(s) 245
BsuTUI ATCGAT 1 cut(s) 307
Cac8I GCNNGC 2 cut(s) 195, 219
ClaI ATCGAT 1 cut(s) 307
CseI GACGC 1 cut(s) 422
Csp6I GTAC 1 cut(s) 542
CviJI RGCY 4 cut(s) 8, 27, 193, 298
CviKI_1 RGCY 4 cut(s) 8, 27, 193, 298
CviQI GTAC 1 cut(s) 542
DdeI CTNAG 3 cut(s) 189, 414, 486
DpnI GATC 1 cut(s) 129
DpnII GATC 1 cut(s) 127
Eam1104I CTCTTC 2 cut(s) 96, 242
EarI CTCTTC 2 cut(s) 96, 242
Eco57I CTGAAG 1 cut(s) 226
EcoRII CCWGG 1 cut(s) 529
FaiI YATR 1 cut(s) 341
FaqI GGGAC 1 cut(s) 195
Fnu4HI GCNGC 5 cut(s) 6, 411, 474, 477, 480
Fsp4HI GCNGC 5 cut(s) 6, 411, 474, 477, 480
GluI GCNGC 5 cut(s) 6, 411, 474, 477, 480
HgaI GACGC 1 cut(s) 422
Hin1I GRCGYC 1 cut(s) 433
HinfI GANTC 4 cut(s) 140, 284, 304, 400
HphI GGTGA 1 cut(s) 36
Hpy166II GTNNAC 1 cut(s) 450
Hpy188I TCNGA 2 cut(s) 190, 289
Hpy188III TCNNGA 5 cut(s) 22, 249, 404, 455, 549
Hpy8I GTNNAC 1 cut(s) 450
HpyAV CCTTC 2 cut(s) 46, 105
HpyCH4IV ACGT 1 cut(s) 394
HpyCH4V TGCA 1 cut(s) 262
HpyF10VI GCNNNNNNNGC 1 cut(s) 479
HpyF3I CTNAG 3 cut(s) 189, 414, 486
HpySE526I ACGT 1 cut(s) 394
Hsp92I GRCGYC 1 cut(s) 433
Kzo9I GATC 1 cut(s) 127
LpnPI CCDG 6 cut(s) 234, 318, 329, 505, 516, 543
Lsp1109I GCAGC 4 cut(s) 397, 485, 488, 491
LweI GCATC 2 cut(s) 397, 516
MaeII ACGT 1 cut(s) 394
MalI GATC 1 cut(s) 129
MboI GATC 1 cut(s) 127
MlyI GAGTC 2 cut(s) 293, 394
MmeI TCCRAC 2 cut(s) 125, 252
MnlI CCTC 8 cut(s) 175, 196, 223, 283, 288, 348, 447, 499
MspR9I CCNGG 1 cut(s) 531
MvaI CCWGG 1 cut(s) 531
MwoI GCNNNNNNNGC 1 cut(s) 479
NdeII GATC 1 cut(s) 127
PcsI WCGNNNNNNNCGW 1 cut(s) 400
PfeI GAWTC 2 cut(s) 140, 304
PkrI GCNGC 5 cut(s) 7, 412, 475, 478, 481
PleI GAGTC 2 cut(s) 292, 394
PpsI GAGTC 2 cut(s) 292, 394
Psp6I CCWGG 1 cut(s) 529
PspGI CCWGG 1 cut(s) 529
RsaI GTAC 1 cut(s) 543
RsaNI GTAC 1 cut(s) 542
SatI GCNGC 5 cut(s) 6, 411, 474, 477, 480
Sau3AI GATC 1 cut(s) 127
SchI GAGTC 2 cut(s) 293, 394
ScrFI CCNGG 1 cut(s) 531
SetI ASST 6 cut(s) 29, 70, 195, 397, 449, 547
SfaNI GCATC 2 cut(s) 397, 516
SmlI CTYRAG 2 cut(s) 438, 453
SmoI CTYRAG 2 cut(s) 438, 453
SsiI CCGC 3 cut(s) 5, 149, 171
StyD4I CCNGG 1 cut(s) 529
TaiI ACGT 1 cut(s) 397
TaqI TCGA 3 cut(s) 138, 153, 307
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 2 cut(s) 140, 304
TseI GCWGC 4 cut(s) 410, 473, 476, 479
TspDTI ATGAA 2 cut(s) 19, 28
TspGWI ACGGA 1 cut(s) 190
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.