RLG00000023458

rRNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0002783
Physical Location & Seq
Forward (+)
24089238 .. 24090378
1141 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000023458

Sequence Viewer

Length: 465 bp
ATGTTTTTCAAGCGATTGTGTAGTAGTTGTAAGGTCCACGATTTTGCACCAAACTCAATCTATGGCTGCCTCATCCTCTCTCTGCAACTTCCTCTCCTTCCTCACACCCTCAAAGCCCCCATCACAACCAAACCCCCACCACCCCATCTCCATCTCTCCCAAACCCATAAGCCTAGAGACGCCTTCATCCCTCTAGTCGCCCAACCTGGTCCTTCTTTCTCTTCGGAATTGAAGTCCGTCATGAACCCCTCTCTGGCTTATGCCAACACTCTCTTCTTCAAGTCGGCTTACAATGTCCAAGTCATCGTCGACGAGGGCGAGTCCGAGGAGCGCCTGCTCAACCGGTTCCGGCGAGAGGTGATGAGAGCTAGCGTCATCCAGGAGGTTAAGAGGAGGAGTTACTTCGAGAACACACAGGATGAGAAGAATCTTCAGTGGAAGAGTTCCATAAAAGGCAGCTGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

155

Amino Acids

17.56

Weight (kDa)

9.41

Isoelectric Point (pI)

51.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 100 - 142 1.5e-10 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 309
AcuI CTGAAG 1 cut(s) 416
AcyI GRCGYC 1 cut(s) 180
AfiI CCNNNNNNNGG 2 cut(s) 253, 355
AgeI ACCGGT 1 cut(s) 342
AgsI TTSAA 3 cut(s) 10, 232, 280
AjnI CCWGG 2 cut(s) 205, 378
AjuI GAANNNNNNNTTGG 2 cut(s) 257, 289
AluBI AGCT 2 cut(s) 368, 459
AluI AGCT 2 cut(s) 368, 459
Alw26I GTCTC 1 cut(s) 171
ApeKI GCWGC 2 cut(s) 66, 456
AsiGI ACCGGT 1 cut(s) 342
AspLEI GCGC 1 cut(s) 333
AspS9I GGNCC 2 cut(s) 34, 209
AsuHPI GGTGA 1 cut(s) 370
AsuNHI GCTAGC 1 cut(s) 368
AvaII GGWCC 2 cut(s) 34, 209
BbvI GCAGC 1 cut(s) 53
BccI CCATC 3 cut(s) 128, 153, 159
BciT130I CCWGG 2 cut(s) 207, 380
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 3 cut(s) 174, 194, 369
BfoI RGCGCY 1 cut(s) 334
BisI GCNGC 2 cut(s) 67, 457
BlsI GCNGC 2 cut(s) 68, 458
Bme1390I CCNGG 2 cut(s) 207, 380
Bme18I GGWCC 2 cut(s) 34, 209
BmgT120I GGNCC 2 cut(s) 34, 209
BmiI GGNNCC 1 cut(s) 347
BmrFI CCNGG 2 cut(s) 207, 380
BmtI GCTAGC 1 cut(s) 372
BsaHI GRCGYC 1 cut(s) 180
BsaJI CCNNGG 1 cut(s) 324
BsaWI WCCGGW 1 cut(s) 342
BsaXI ACNNNNNCTCC 4 cut(s) 78, 108, 132, 162
Bsc4I CCNNNNNNNGG 2 cut(s) 253, 355
Bse118I RCCGGY 1 cut(s) 342
BseBI CCWGG 2 cut(s) 207, 380
BseDI CCNNGG 1 cut(s) 324
BseGI GGATG 4 cut(s) 72, 186, 375, 424
BseLI CCNNNNNNNGG 2 cut(s) 253, 355
BseRI GAGGAG 3 cut(s) 341, 406, 409
BseXI GCAGC 1 cut(s) 53
BshTI ACCGGT 1 cut(s) 342
BsiSI CCGG 2 cut(s) 343, 349
BslI CCNNNNNNNGG 2 cut(s) 253, 355
BsmAI GTCTC 1 cut(s) 171
BsmBI CGTCTC 1 cut(s) 171
BspHI TCATGA 1 cut(s) 240
BspLI GGNNCC 1 cut(s) 347
BspOI GCTAGC 1 cut(s) 372
BsrFI RCCGGY 1 cut(s) 342
BssAI RCCGGY 1 cut(s) 342
BssECI CCNNGG 1 cut(s) 324
BssNI GRCGYC 1 cut(s) 180
Bst2UI CCWGG 2 cut(s) 207, 380
Bst6I CTCTTC 3 cut(s) 226, 278, 434
BstACI GRCGYC 1 cut(s) 180
BstC8I GCNNGC 2 cut(s) 335, 370
BstF5I GGATG 4 cut(s) 72, 186, 375, 424
BstH2I RGCGCY 1 cut(s) 334
BstHHI GCGC 1 cut(s) 333
BstMAI GTCTC 1 cut(s) 171
BstNI CCWGG 2 cut(s) 207, 380
BstSCI CCNGG 2 cut(s) 205, 378
BstV1I GCAGC 1 cut(s) 53
BtsCI GGATG 4 cut(s) 72, 186, 375, 424
BtsIMutI CAGTG 1 cut(s) 440
Cac8I GCNNGC 2 cut(s) 335, 370
CciI TCATGA 1 cut(s) 240
CfoI GCGC 1 cut(s) 333
Cfr10I RCCGGY 1 cut(s) 342
Cfr13I GGNCC 2 cut(s) 34, 209
CseI GACGC 2 cut(s) 188, 361
CsiI ACCWGGT 1 cut(s) 205
CspAI ACCGGT 1 cut(s) 342
CviAII CATG 1 cut(s) 241
CviJI RGCY 7 cut(s) 66, 116, 172, 257, 287, 368, 459
CviKI_1 RGCY 7 cut(s) 66, 116, 172, 257, 287, 368, 459
Eam1104I CTCTTC 3 cut(s) 226, 278, 434
EarI CTCTTC 3 cut(s) 226, 278, 434
Eco47I GGWCC 2 cut(s) 34, 209
Eco57I CTGAAG 1 cut(s) 416
EcoRII CCWGG 2 cut(s) 205, 378
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 1 cut(s) 244
FaiI YATR 5 cut(s) 63, 168, 242, 261, 449
FatI CATG 1 cut(s) 240
FblI GTMKAC 1 cut(s) 309
Fnu4HI GCNGC 2 cut(s) 67, 457
FokI GGATG 4 cut(s) 59, 173, 362, 431
Fsp4HI GCNGC 2 cut(s) 67, 457
FspBI CTAG 3 cut(s) 174, 194, 369
GlaI GCGC 1 cut(s) 332
GluI GCNGC 2 cut(s) 67, 457
HaeII RGCGCY 1 cut(s) 334
HapII CCGG 2 cut(s) 343, 349
HgaI GACGC 2 cut(s) 188, 361
HhaI GCGC 1 cut(s) 333
Hin1I GRCGYC 1 cut(s) 180
Hin1II CATG 1 cut(s) 244
Hin6I GCGC 1 cut(s) 331
HinP1I GCGC 1 cut(s) 331
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HinfI GANTC 2 cut(s) 320, 427
HpaII CCGG 2 cut(s) 343, 349
HphI GGTGA 1 cut(s) 370
Hpy166II GTNNAC 2 cut(s) 37, 310
Hpy188I TCNGA 2 cut(s) 226, 325
Hpy188III TCNNGA 2 cut(s) 241, 406
Hpy8I GTNNAC 2 cut(s) 37, 310
Hpy99I CGWCG 2 cut(s) 311, 314
HpyAV CCTTC 3 cut(s) 107, 193, 222
HpyCH4V TGCA 2 cut(s) 47, 85
Hsp92I GRCGYC 1 cut(s) 180
Hsp92II CATG 1 cut(s) 244
HspAI GCGC 1 cut(s) 331
LmnI GCTCC 1 cut(s) 328
LpnPI CCDG 9 cut(s) 192, 219, 239, 347, 356, 362, 365, 392, 401
Lsp1109I GCAGC 1 cut(s) 53
MabI ACCWGGT 1 cut(s) 205
MaeI CTAG 3 cut(s) 174, 194, 369
MaeIII GTNAC 1 cut(s) 398
MboII GAAGA 6 cut(s) 213, 265, 268, 422, 436, 451
MluCI AATT 1 cut(s) 227
MlyI GAGTC 1 cut(s) 329
MseI TTAA 2 cut(s) 387, 463
MspA1I CMGCKG 1 cut(s) 459
MspI CCGG 2 cut(s) 343, 349
MspR9I CCNGG 2 cut(s) 207, 380
MvaI CCWGG 2 cut(s) 207, 380
NheI GCTAGC 1 cut(s) 368
NlaIII CATG 1 cut(s) 244
NlaIV GGNNCC 1 cut(s) 347
PagI TCATGA 1 cut(s) 240
PcsI WCGNNNNNNNCGW 1 cut(s) 315
PfeI GAWTC 1 cut(s) 427
PfoI TCCNGGA 1 cut(s) 378
PinAI ACCGGT 1 cut(s) 342
PkrI GCNGC 2 cut(s) 68, 458
PleI GAGTC 1 cut(s) 328
PpsI GAGTC 1 cut(s) 328
Psp6I CCWGG 2 cut(s) 205, 378
PspGI CCWGG 2 cut(s) 205, 378
PspN4I GGNNCC 1 cut(s) 347
PspPI GGNCC 2 cut(s) 34, 209
PvuII CAGCTG 1 cut(s) 459
SalI GTCGAC 1 cut(s) 308
SaqAI TTAA 2 cut(s) 387, 463
SatI GCNGC 2 cut(s) 67, 457
Sau96I GGNCC 2 cut(s) 34, 209
SchI GAGTC 1 cut(s) 329
ScrFI CCNGG 2 cut(s) 207, 380
SetI ASST 6 cut(s) 36, 208, 360, 370, 387, 461
SexAI ACCWGGT 1 cut(s) 205
SgrDI CGTCGACG 1 cut(s) 308
SinI GGWCC 2 cut(s) 34, 209
Sse9I AATT 1 cut(s) 227
SspMI CTAG 3 cut(s) 174, 194, 369
StyD4I CCNGG 2 cut(s) 205, 378
TaqI TCGA 2 cut(s) 309, 405
TasI AATT 1 cut(s) 227
TfiI GAWTC 1 cut(s) 427
Tru1I TTAA 2 cut(s) 387, 463
Tru9I TTAA 2 cut(s) 387, 463
TscAI CASTG 1 cut(s) 440
TseI GCWGC 2 cut(s) 66, 456
TspDTI ATGAA 2 cut(s) 175, 257
TspGWI ACGGA 1 cut(s) 226
TspRI CASTG 1 cut(s) 440
VpaK11BI GGWCC 2 cut(s) 34, 209
XmiI GTMKAC 1 cut(s) 309
XspI CTAG 3 cut(s) 174, 194, 369
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.