Rw3G008700

rRNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0002783
Physical Location & Seq
Reverse (-)
8050808 .. 8051383
576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G008700.1

Sequence Viewer

Length: 576 bp
ATGGAAGTCTTCCACTGTAGCAGACAAGTACCAGCTAGGCCTTTCGCTTTCTTCCTTCTTCTTCAATCTCACGAATTTGTTCGTCTTCACGCCCATGATAGAGAGGGAAACCAATATCAGAAGGTCCACGATTTTGCACCAAACTCAATCCATGGATGCCTCATCCTCTCTCTGCAACTTCCTCTCCTTCCTCACACCCTCAAAGCCCCCATCACAAGCCAAACCCCCACCACCCCATCTCCATCTCTCCCAAACCCAGAAGCCCAGAGAGGCAGAGACGCCTTCGTCCCTCTAGTCACCCAACCTGGTCCTTCTTTCTCTTCTTCCTCTTCGGAATTGGAGTCCTTCATGAACGCCTCTCTGGCTTATGCCAACACTATCTTTTTCAAGTCGGCTTACAATGTCCAAGTCATCGTCGACGAGGGTGAGTCTGAGGAGCGCCTGCTCAACCGGTTCAGGCGAGAGGTGATGAGAGCTGGCGTCATCCAGGAGGTTAAGAGGGAGGAGGTGTACTTCCAGAACACACAAGATGAGAAGAAGCTACAATGGGAGAGTTCCATAAAAGGCAGCTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

191

Amino Acids

21.76

Weight (kDa)

6.38

Isoelectric Point (pI)

49.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 136 - 184 3.1e-09 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 284
AccI GTMKAC 1 cut(s) 417
AcsI RAATTY 1 cut(s) 74
AcyI GRCGYC 2 cut(s) 279, 480
AfaI GTAC 2 cut(s) 30, 512
AgeI ACCGGT 1 cut(s) 450
AgsI TTSAA 2 cut(s) 65, 388
AjnI CCWGG 2 cut(s) 304, 486
AluBI AGCT 4 cut(s) 35, 476, 541, 570
AluI AGCT 4 cut(s) 35, 476, 541, 570
Alw26I GTCTC 1 cut(s) 270
AoxI GGCC 1 cut(s) 38
ApeKI GCWGC 1 cut(s) 567
ApoI RAATTY 1 cut(s) 74
AsiGI ACCGGT 1 cut(s) 450
Asp700I GAANNNNTTC 1 cut(s) 78
AspLEI GCGC 1 cut(s) 441
AspS9I GGNCC 2 cut(s) 124, 308
AsuHPI GGTGA 3 cut(s) 289, 437, 478
AvaII GGWCC 2 cut(s) 124, 308
BbsI GAAGAC 1 cut(s) 77
BccI CCATC 3 cut(s) 218, 244, 250
BciT130I CCWGG 2 cut(s) 306, 488
BcoDI GTCTC 1 cut(s) 270
BfaI CTAG 2 cut(s) 36, 293
BfmI CTRYAG 1 cut(s) 16
BfoI RGCGCY 1 cut(s) 442
BglI GCCNNNNNGGC 1 cut(s) 362
BisI GCNGC 1 cut(s) 568
BlsI GCNGC 1 cut(s) 569
Bme1390I CCNGG 2 cut(s) 306, 488
Bme18I GGWCC 2 cut(s) 124, 308
BmgT120I GGNCC 2 cut(s) 124, 308
BmrFI CCNGG 2 cut(s) 306, 488
BmsI GCATC 1 cut(s) 146
BpiI GAAGAC 1 cut(s) 77
BsaHI GRCGYC 2 cut(s) 279, 480
BsaJI CCNNGG 1 cut(s) 151
BsaWI WCCGGW 1 cut(s) 450
BsaXI ACNNNNNCTCC 6 cut(s) 168, 198, 223, 253, 494, 524
Bse118I RCCGGY 1 cut(s) 450
BseBI CCWGG 2 cut(s) 306, 488
BseDI CCNNGG 1 cut(s) 151
BseGI GGATG 3 cut(s) 161, 162, 483
BseMII CTCAG 1 cut(s) 423
BseRI GAGGAG 2 cut(s) 449, 518
BshFI GGCC 1 cut(s) 40
BshTI ACCGGT 1 cut(s) 450
BsiSI CCGG 1 cut(s) 451
BslFI GGGAC 1 cut(s) 272
BsmAI GTCTC 1 cut(s) 270
BsmBI CGTCTC 1 cut(s) 270
BsmFI GGGAC 1 cut(s) 272
BsnI GGCC 1 cut(s) 40
Bsp19I CCATGG 1 cut(s) 151
BspANI GGCC 1 cut(s) 40
BspCNI CTCAG 1 cut(s) 424
BspHI TCATGA 1 cut(s) 348
BsrFI RCCGGY 1 cut(s) 450
BssAI RCCGGY 1 cut(s) 450
BssECI CCNNGG 1 cut(s) 151
BssNI GRCGYC 2 cut(s) 279, 480
BssT1I CCWWGG 1 cut(s) 151
Bst2UI CCWGG 2 cut(s) 306, 488
Bst4CI ACNGT 1 cut(s) 17
Bst6I CTCTTC 2 cut(s) 325, 334
BstACI GRCGYC 2 cut(s) 279, 480
BstC8I GCNNGC 2 cut(s) 443, 478
BstDEI CTNAG 1 cut(s) 432
BstDSI CCRYGG 1 cut(s) 151
BstF5I GGATG 3 cut(s) 161, 162, 483
BstH2I RGCGCY 1 cut(s) 442
BstHHI GCGC 1 cut(s) 441
BstMAI GTCTC 1 cut(s) 270
BstMWI GCNNNNNNNGC 1 cut(s) 362
BstNI CCWGG 2 cut(s) 306, 488
BstSCI CCNGG 2 cut(s) 304, 486
BstSFI CTRYAG 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 77
BsuRI GGCC 1 cut(s) 40
BtgI CCRYGG 1 cut(s) 151
BtsCI GGATG 3 cut(s) 161, 162, 483
BtsIMutI CAGTG 1 cut(s) 13
Cac8I GCNNGC 2 cut(s) 443, 478
CciI TCATGA 1 cut(s) 348
CfoI GCGC 1 cut(s) 441
Cfr10I RCCGGY 1 cut(s) 450
Cfr13I GGNCC 2 cut(s) 124, 308
CseI GACGC 2 cut(s) 287, 469
CsiI ACCWGGT 1 cut(s) 304
Csp6I GTAC 2 cut(s) 29, 511
CspAI ACCGGT 1 cut(s) 450
CviAII CATG 3 cut(s) 95, 152, 349
CviQI GTAC 2 cut(s) 29, 511
DdeI CTNAG 1 cut(s) 432
DrdI GACNNNNNNGTC 1 cut(s) 284
DseDI GACNNNNNNGTC 1 cut(s) 284
Eam1104I CTCTTC 2 cut(s) 325, 334
EarI CTCTTC 2 cut(s) 325, 334
Eco130I CCWWGG 1 cut(s) 151
Eco147I AGGCCT 1 cut(s) 40
Eco47I GGWCC 2 cut(s) 124, 308
EcoRII CCWGG 2 cut(s) 304, 486
EcoT14I CCWWGG 1 cut(s) 151
ErhI CCWWGG 1 cut(s) 151
Esp3I CGTCTC 1 cut(s) 270
FaeI CATG 3 cut(s) 98, 155, 352
FaiI YATR 5 cut(s) 96, 153, 350, 369, 560
FaqI GGGAC 1 cut(s) 272
FatI CATG 3 cut(s) 94, 151, 348
FblI GTMKAC 1 cut(s) 417
Fnu4HI GCNGC 1 cut(s) 568
FokI GGATG 3 cut(s) 149, 168, 470
Fsp4HI GCNGC 1 cut(s) 568
FspBI CTAG 2 cut(s) 36, 293
GlaI GCGC 1 cut(s) 440
GluI GCNGC 1 cut(s) 568
HaeII RGCGCY 1 cut(s) 442
HaeIII GGCC 1 cut(s) 40
HapII CCGG 1 cut(s) 451
HgaI GACGC 2 cut(s) 287, 469
HhaI GCGC 1 cut(s) 441
Hin1I GRCGYC 2 cut(s) 279, 480
Hin1II CATG 3 cut(s) 98, 155, 352
Hin6I GCGC 1 cut(s) 439
HinP1I GCGC 1 cut(s) 439
HincII GTYRAC 1 cut(s) 418
HindII GTYRAC 1 cut(s) 418
HinfI GANTC 2 cut(s) 341, 428
HpaII CCGG 1 cut(s) 451
HphI GGTGA 3 cut(s) 289, 437, 478
Hpy166II GTNNAC 3 cut(s) 127, 418, 511
Hpy188I TCNGA 3 cut(s) 120, 334, 433
Hpy188III TCNNGA 3 cut(s) 71, 349, 517
Hpy8I GTNNAC 3 cut(s) 127, 418, 511
Hpy99I CGWCG 2 cut(s) 419, 422
HpyAV CCTTC 6 cut(s) 65, 115, 197, 292, 321, 355
HpyCH4III ACNGT 1 cut(s) 17
HpyCH4V TGCA 2 cut(s) 137, 175
HpyF10VI GCNNNNNNNGC 1 cut(s) 362
HpyF3I CTNAG 1 cut(s) 432
Hsp92I GRCGYC 2 cut(s) 279, 480
Hsp92II CATG 3 cut(s) 98, 155, 352
HspAI GCGC 1 cut(s) 439
LmnI GCTCC 1 cut(s) 436
LweI GCATC 1 cut(s) 146
MabI ACCWGGT 1 cut(s) 304
MaeI CTAG 2 cut(s) 36, 293
MaeIII GTNAC 1 cut(s) 295
MboII GAAGA 8 cut(s) 43, 50, 53, 77, 312, 315, 321, 547
MluCI AATT 2 cut(s) 74, 335
MlyI GAGTC 2 cut(s) 350, 437
MroXI GAANNNNTTC 1 cut(s) 78
MseI TTAA 2 cut(s) 495, 574
MslI CAYNNNNRTG 1 cut(s) 93
MspI CCGG 1 cut(s) 451
MspR9I CCNGG 2 cut(s) 306, 488
MvaI CCWGG 2 cut(s) 306, 488
MwoI GCNNNNNNNGC 1 cut(s) 362
NcoI CCATGG 1 cut(s) 151
NlaIII CATG 3 cut(s) 98, 155, 352
NmuCI GTSAC 1 cut(s) 295
PagI TCATGA 1 cut(s) 348
PceI AGGCCT 1 cut(s) 40
PdmI GAANNNNTTC 1 cut(s) 78
PfoI TCCNGGA 1 cut(s) 486
PinAI ACCGGT 1 cut(s) 450
PkrI GCNGC 1 cut(s) 569
PleI GAGTC 2 cut(s) 349, 436
PpsI GAGTC 2 cut(s) 349, 436
Psp6I CCWGG 2 cut(s) 304, 486
PspGI CCWGG 2 cut(s) 304, 486
PspPI GGNCC 2 cut(s) 124, 308
RsaI GTAC 2 cut(s) 30, 512
RsaNI GTAC 2 cut(s) 29, 511
RseI CAYNNNNRTG 1 cut(s) 93
SalI GTCGAC 1 cut(s) 416
SaqAI TTAA 2 cut(s) 495, 574
SatI GCNGC 1 cut(s) 568
Sau96I GGNCC 2 cut(s) 124, 308
SchI GAGTC 2 cut(s) 350, 437
ScrFI CCNGG 2 cut(s) 306, 488
SetI ASST 9 cut(s) 37, 126, 307, 468, 478, 495, 510, 543, 572
SexAI ACCWGGT 1 cut(s) 304
SfaNI GCATC 1 cut(s) 146
SfcI CTRYAG 1 cut(s) 16
SgrDI CGTCGACG 1 cut(s) 416
SinI GGWCC 2 cut(s) 124, 308
SmiMI CAYNNNNRTG 1 cut(s) 93
Sse9I AATT 2 cut(s) 74, 335
SseBI AGGCCT 1 cut(s) 40
SspMI CTAG 2 cut(s) 36, 293
StuI AGGCCT 1 cut(s) 40
StyD4I CCNGG 2 cut(s) 304, 486
StyI CCWWGG 1 cut(s) 151
TaaI ACNGT 1 cut(s) 17
TaqI TCGA 1 cut(s) 417
TasI AATT 2 cut(s) 74, 335
TatI WGTACW 1 cut(s) 510
Tru1I TTAA 2 cut(s) 495, 574
Tru9I TTAA 2 cut(s) 495, 574
TscAI CASTG 1 cut(s) 20
TseFI GTSAC 1 cut(s) 295
TseI GCWGC 1 cut(s) 567
Tsp45I GTSAC 1 cut(s) 295
TspDTI ATGAA 2 cut(s) 337, 365
TspRI CASTG 1 cut(s) 20
VpaK11BI GGWCC 2 cut(s) 124, 308
XapI RAATTY 1 cut(s) 74
XmiI GTMKAC 1 cut(s) 417
XmnI GAANNNNTTC 1 cut(s) 78
XspI CTAG 2 cut(s) 36, 293
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.