MD17G1012600.v1.1

rRNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr17
OG0002783
Physical Location & Seq
Forward (+)
1108674 .. 1110294
1621 bp
Loading structure...
UTR
Exon/CDS
Intron
MD17G1012600.v1.1.491

Sequence Viewer

Length: 522 bp
ATGGCTGCCTCATCCGCTCTCTGCAACTTGTTCTCCCTCCTCAGCCCCTCAAAGCCACCGCAACCCAAACTCCCACCGACCCAGCTCTCCATTTTACAGACCCAGAAGCCCAGAGACCGGCTGGTTCCACTGGCTGCCCAAGGGGCCCACTCTGCTTCTTCTTCTTCGTCGATAGAGTCTGTGATTTGCCCCTCTCTGGCTCACGCCAACGTCCTCTTCTTCAAGTCGGCGTACAATGTGCAGGTGATTGTTGACGACAACGAGCCCGAGGAGCGGCTGCTCGGCAGGTTCCGGCGGGAGGTCATGAAGGCCGGAGTTATTCAGGAGGTTAAGAGGAGGAGGTACTTTGAGAACAAGCAGGATGAAAAGAAGCGCCGGACCCGCGACGCCGCGAAGCGAAACAAGAGAAGCCGGCGACCCTTCTCAAGACCCTTTCAGCAGAAGCCAGAAGTCCCTGAAGCCAAGAAGAGCGATGATGACGGAGATAACTGGGATCTTCCTCAAGGAGGCATACCTTACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

174

Amino Acids

19.58

Weight (kDa)

9.95

Isoelectric Point (pI)

77.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 81 - 134 3.5e-14 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 232
Acc36I ACCTGC 2 cut(s) 232, 276
AccBSI CCGCTC 2 cut(s) 17, 274
AccII CGCG 2 cut(s) 384, 392
AciI CCGC 6 cut(s) 15, 59, 274, 295, 382, 390
AclWI GGATC 1 cut(s) 501
AcuI CTGAAG 1 cut(s) 477
AcyI GRCGYC 1 cut(s) 387
AfaI GTAC 2 cut(s) 233, 344
AfiI CCNNNNNNNGG 5 cut(s) 117, 196, 273, 298, 506
AgsI TTSAA 1 cut(s) 223
AjuI GAANNNNNNNTTGG 2 cut(s) 200, 232
AluBI AGCT 1 cut(s) 85
AluI AGCT 1 cut(s) 85
Alw26I GTCTC 1 cut(s) 108
AlwI GGATC 1 cut(s) 501
Ama87I CYCGRG 1 cut(s) 266
AoxI GGCC 2 cut(s) 144, 309
ApaI GGGCCC 1 cut(s) 148
ApeKI GCWGC 3 cut(s) 5, 134, 277
AspLEI GCGC 1 cut(s) 375
AspS9I GGNCC 3 cut(s) 144, 145, 378
AsuHPI GGTGA 1 cut(s) 256
AvaI CYCGRG 1 cut(s) 266
AvaII GGWCC 1 cut(s) 378
BaeGI GKGCMC 1 cut(s) 148
BanII GRGCYC 2 cut(s) 148, 267
BbvCI CCTCAGC 1 cut(s) 41
BbvI GCAGC 2 cut(s) 121, 264
BcoDI GTCTC 1 cut(s) 108
BfoI RGCGCY 1 cut(s) 376
BfuAI ACCTGC 2 cut(s) 232, 276
BglI GCCNNNNNGGC 1 cut(s) 143
BisI GCNGC 5 cut(s) 6, 135, 275, 278, 390
BlsI GCNGC 5 cut(s) 7, 136, 276, 279, 391
Bme18I GGWCC 1 cut(s) 378
BmeT110I CYCGRG 1 cut(s) 266
BmgT120I GGNCC 3 cut(s) 144, 145, 378
BmiI GGNNCC 5 cut(s) 126, 145, 146, 290, 380
BmrI ACTGGG 1 cut(s) 499
BmuI ACTGGG 1 cut(s) 499
Bpu10I CCTNAGC 1 cut(s) 41
BpuEI CTTGAG 2 cut(s) 409, 486
BsaHI GRCGYC 1 cut(s) 387
BsaI GGTCTC 1 cut(s) 108
BsaJI CCNNGG 2 cut(s) 139, 267
BsaXI ACNNNNNCTCC 4 cut(s) 17, 47, 54, 84
Bsc4I CCNNNNNNNGG 5 cut(s) 117, 196, 273, 298, 506
Bse118I RCCGGY 2 cut(s) 117, 411
Bse1I ACTGG 2 cut(s) 135, 494
BseDI CCNNGG 2 cut(s) 139, 267
BseGI GGATG 2 cut(s) 11, 367
BseLI CCNNNNNNNGG 5 cut(s) 117, 196, 273, 298, 506
BseMII CTCAG 1 cut(s) 55
BseNI ACTGG 2 cut(s) 135, 494
BseRI GAGGAG 4 cut(s) 29, 284, 349, 352
BseSI GKGCMC 1 cut(s) 148
BseXI GCAGC 2 cut(s) 121, 264
BseYI CCCAGC 1 cut(s) 81
BsgI GTGCAG 1 cut(s) 260
Bsh1236I CGCG 2 cut(s) 384, 392
BshFI GGCC 2 cut(s) 146, 311
BsiHKCI CYCGRG 1 cut(s) 266
BsiSI CCGG 5 cut(s) 118, 292, 312, 376, 412
BslFI GGGAC 1 cut(s) 437
BslI CCNNNNNNNGG 5 cut(s) 117, 196, 273, 298, 506
BsmAI GTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 437
BsnI GGCC 2 cut(s) 146, 311
Bso31I GGTCTC 1 cut(s) 108
BsoBI CYCGRG 1 cut(s) 266
Bsp120I GGGCCC 1 cut(s) 144
Bsp1286I GDGCHC 2 cut(s) 148, 267
Bsp143I GATC 1 cut(s) 493
BspACI CCGC 6 cut(s) 15, 59, 274, 295, 382, 390
BspANI GGCC 2 cut(s) 146, 311
BspCNI CTCAG 1 cut(s) 54
BspFNI CGCG 2 cut(s) 384, 392
BspHI TCATGA 1 cut(s) 303
BspLI GGNNCC 5 cut(s) 126, 145, 146, 290, 380
BspMI ACCTGC 2 cut(s) 232, 276
BspPI GGATC 1 cut(s) 501
BspQI GCTCTTC 1 cut(s) 461
BspTNI GGTCTC 1 cut(s) 108
BsrBI CCGCTC 2 cut(s) 17, 274
BsrFI RCCGGY 2 cut(s) 117, 411
BsrI ACTGG 2 cut(s) 135, 494
BssAI RCCGGY 2 cut(s) 117, 411
BssECI CCNNGG 2 cut(s) 139, 267
BssMI GATC 1 cut(s) 493
BssNI GRCGYC 1 cut(s) 387
BssT1I CCWWGG 1 cut(s) 139
Bst6I CTCTTC 2 cut(s) 221, 461
BstACI GRCGYC 1 cut(s) 387
BstC8I GCNNGC 1 cut(s) 413
BstDEI CTNAG 1 cut(s) 41
BstENI CCTNNNNNAGG 1 cut(s) 504
BstF5I GGATG 2 cut(s) 11, 367
BstFNI CGCG 2 cut(s) 384, 392
BstH2I RGCGCY 1 cut(s) 376
BstHHI GCGC 1 cut(s) 375
BstKTI GATC 1 cut(s) 496
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 1 cut(s) 493
BstMWI GCNNNNNNNGC 5 cut(s) 14, 143, 152, 271, 381
BstSLI GKGCMC 1 cut(s) 148
BstUI CGCG 2 cut(s) 384, 392
BstV1I GCAGC 2 cut(s) 121, 264
BstX2I RGATCY 1 cut(s) 493
BstYI RGATCY 1 cut(s) 493
BsuRI GGCC 2 cut(s) 146, 311
BtgZI GCGATG 1 cut(s) 486
BtsCI GGATG 2 cut(s) 11, 367
BtsIMutI CAGTG 1 cut(s) 128
BveI ACCTGC 2 cut(s) 232, 276
Cac8I GCNNGC 1 cut(s) 413
CciI TCATGA 1 cut(s) 303
CfoI GCGC 1 cut(s) 375
Cfr10I RCCGGY 2 cut(s) 117, 411
Cfr13I GGNCC 3 cut(s) 144, 145, 378
CseI GACGC 1 cut(s) 395
Csp6I GTAC 2 cut(s) 232, 343
CviAII CATG 1 cut(s) 304
CviQI GTAC 2 cut(s) 232, 343
DdeI CTNAG 1 cut(s) 41
DpnI GATC 1 cut(s) 495
DpnII GATC 1 cut(s) 493
Eam1104I CTCTTC 2 cut(s) 221, 461
EarI CTCTTC 2 cut(s) 221, 461
Eco130I CCWWGG 1 cut(s) 139
Eco24I GRGCYC 2 cut(s) 148, 267
Eco31I GGTCTC 1 cut(s) 108
Eco47I GGWCC 1 cut(s) 378
Eco57I CTGAAG 1 cut(s) 477
Eco88I CYCGRG 1 cut(s) 266
EcoNI CCTNNNNNAGG 1 cut(s) 504
EcoO109I RGGNCCY 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 139
EcoT38I GRGCYC 2 cut(s) 148, 267
ErhI CCWWGG 1 cut(s) 139
FaeI CATG 1 cut(s) 307
FaiI YATR 2 cut(s) 305, 512
FaqI GGGAC 1 cut(s) 437
FatI CATG 1 cut(s) 303
FauI CCCGC 2 cut(s) 288, 389
Fnu4HI GCNGC 5 cut(s) 6, 135, 275, 278, 390
FokI GGATG 1 cut(s) 374
FriOI GRGCYC 2 cut(s) 148, 267
Fsp4HI GCNGC 5 cut(s) 6, 135, 275, 278, 390
GlaI GCGC 1 cut(s) 374
GluI GCNGC 5 cut(s) 6, 135, 275, 278, 390
GsaI CCCAGC 1 cut(s) 85
HaeII RGCGCY 1 cut(s) 376
HaeIII GGCC 2 cut(s) 146, 311
HapII CCGG 5 cut(s) 118, 292, 312, 376, 412
HgaI GACGC 1 cut(s) 395
HhaI GCGC 1 cut(s) 375
Hin1I GRCGYC 1 cut(s) 387
Hin1II CATG 1 cut(s) 307
Hin6I GCGC 1 cut(s) 373
HinP1I GCGC 1 cut(s) 373
HincII GTYRAC 1 cut(s) 253
HindII GTYRAC 1 cut(s) 253
HinfI GANTC 1 cut(s) 176
HpaII CCGG 5 cut(s) 118, 292, 312, 376, 412
HphI GGTGA 1 cut(s) 256
Hpy166II GTNNAC 1 cut(s) 253
Hpy188III TCNNGA 3 cut(s) 304, 323, 426
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 2 cut(s) 172, 389
HpyAV CCTTC 2 cut(s) 301, 430
HpyCH4IV ACGT 1 cut(s) 210
HpyCH4V TGCA 2 cut(s) 24, 241
HpyF10VI GCNNNNNNNGC 5 cut(s) 14, 143, 152, 271, 381
HpyF3I CTNAG 1 cut(s) 41
HpySE526I ACGT 1 cut(s) 210
Hsp92I GRCGYC 1 cut(s) 387
Hsp92II CATG 1 cut(s) 307
HspAI GCGC 1 cut(s) 373
KroI GCCGGC 1 cut(s) 411
KroNI GCCGGC 1 cut(s) 413
Kzo9I GATC 1 cut(s) 493
LguI GCTCTTC 1 cut(s) 461
LmnI GCTCC 1 cut(s) 271
Lsp1109I GCAGC 2 cut(s) 121, 264
MaeII ACGT 1 cut(s) 210
MalI GATC 1 cut(s) 495
MbiI CCGCTC 2 cut(s) 17, 274
MboI GATC 1 cut(s) 493
MboII GAAGA 7 cut(s) 150, 153, 156, 208, 211, 478, 488
MflI RGATCY 1 cut(s) 493
MhlI GDGCHC 2 cut(s) 148, 267
MlyI GAGTC 1 cut(s) 185
MroNI GCCGGC 1 cut(s) 411
MseI TTAA 1 cut(s) 330
MspI CCGG 5 cut(s) 118, 292, 312, 376, 412
MvnI CGCG 2 cut(s) 384, 392
MwoI GCNNNNNNNGC 5 cut(s) 14, 143, 152, 271, 381
NaeI GCCGGC 1 cut(s) 413
NdeII GATC 1 cut(s) 493
NgoMIV GCCGGC 1 cut(s) 411
NlaIII CATG 1 cut(s) 307
NlaIV GGNNCC 5 cut(s) 126, 145, 146, 290, 380
NmeAIII GCCGAG 1 cut(s) 261
PagI TCATGA 1 cut(s) 303
PaqCI CACCTGC 1 cut(s) 232
PciSI GCTCTTC 1 cut(s) 461
PdiI GCCGGC 1 cut(s) 413
PkrI GCNGC 5 cut(s) 7, 136, 276, 279, 391
PleI GAGTC 1 cut(s) 184
PpsI GAGTC 1 cut(s) 184
PspFI CCCAGC 1 cut(s) 81
PspN4I GGNNCC 5 cut(s) 126, 145, 146, 290, 380
PspOMI GGGCCC 1 cut(s) 144
PspPI GGNCC 3 cut(s) 144, 145, 378
PsuI RGATCY 1 cut(s) 493
RsaI GTAC 2 cut(s) 233, 344
RsaNI GTAC 2 cut(s) 232, 343
SapI GCTCTTC 1 cut(s) 461
SaqAI TTAA 1 cut(s) 330
SatI GCNGC 5 cut(s) 6, 135, 275, 278, 390
Sau3AI GATC 1 cut(s) 493
Sau96I GGNCC 3 cut(s) 144, 145, 378
SchI GAGTC 1 cut(s) 185
SduI GDGCHC 2 cut(s) 148, 267
SetI ASST 8 cut(s) 87, 213, 246, 290, 303, 330, 344, 517
SinI GGWCC 1 cut(s) 378
SmlI CTYRAG 2 cut(s) 424, 501
SmoI CTYRAG 2 cut(s) 424, 501
SsiI CCGC 6 cut(s) 15, 59, 274, 295, 382, 390
StyI CCWWGG 1 cut(s) 139
TaiI ACGT 1 cut(s) 213
TaqI TCGA 1 cut(s) 170
TauI GCSGC 2 cut(s) 277, 392
Tru1I TTAA 1 cut(s) 330
Tru9I TTAA 1 cut(s) 330
TscAI CASTG 1 cut(s) 135
TseI GCWGC 3 cut(s) 5, 134, 277
TspDTI ATGAA 2 cut(s) 320, 378
TspGWI ACGGA 1 cut(s) 495
TspRI CASTG 1 cut(s) 135
VpaK11BI GGWCC 1 cut(s) 378
XagI CCTNNNNNAGG 1 cut(s) 504
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.