Rorug06G0509100

rRNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002783
Physical Location & Seq
Forward (+)
62355884 .. 62365027
9144 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0509100.1

Sequence Viewer

Length: 5862 bp
ATGGACGACGAGCTACCGACCCAGGGCCAAGGCCAAGCCGCGCCACCAGCTCCGCCGCCTCCCCCGGCGCCGGAATCGCAGGCAGGCGAGGAGCCGGAGGAAGACGACGAAGACGAGGAGCCGAAGAATGAAGACGATGAAATGGTGGCCAAGGCTCAGAAGCTCATGGATAAGGTTATTGCTTCGCCTGATAACCCCAGCCCCACTGTTCTTCACGCCCTCGCTTCACTTCTTGAAACGCAGGAGAAACGATACATGGAGGAGAGTGGTCATTCTTCCAGCAATGGTCGTGCTTCTCATAACATTGGACGGCTAGGGAACTTGGTTCGGGAACATGATGACTTCTTTGAATTAATATCTGCCAAGTATTTGTCAGATACGAGATACTCTGCGTCCGTCCAGGCAGCTGCTGCAAGGCTGTTTCTGAGCTGTTCACTGACTTTGATTTATCCTCATGTTTTTGAAGAAGACGTTTTGGAGAAAATAAAGGACTGGGTGATGGATGAGACTTCCAGTGTCTCGGTTGAATACCAGAACTGGAAGCATGATTTGGGAGGAAGGGAGGTCTCAGATTTTGAAATGCTGAAGACATATTCTACTGGACTTCTTGCTGTTTGTTTGACTGGGGGTGGTCAGGTAGTGGAAGATGTGTTGACGTCCGGATTGTCAGCCAAACTTATGCGTTATCTTCGTGTCCGTGTTTTGGGGGAGTCGAGTATTAGTCAGAAGGATTCTAGTCATTTAACAGAGACTAAAAACACCTCTGGTGTAAGAGGTAGAGATGAAGGTCGGGGTAGGGTTCGGCAGGTTCTGGAAACAACTCATTTTGATGATCCAAGGGTAACAAGTGAGAGATGCTTGGATGACCAGACTGTTGAAGGGAACCAGGACAGAAGTATCTGTAGGCAAGCGTTTGGAGGAGAACGTTGGGTTGATGATGGAGAACCACCTGATGGAATGGATGAAGGGGTTGAGATCAATGATGTTGATGGCAGTGATTCTCGTGATTTACGTGATGGGAAACAAAAATTTGGTGATTTTGATGAAAATGGGAGAGATGACTCCTCAAGGCGCAGACCTAACCGTGGATGGGCACGATCTAGAGGGAAGGGAAGGGCAAACGAAGGCGGTGTAGAAAATGAGCAGCTGTTGACCTCACCCGGATCTGCTATTCGGTTGGGACAGGGGCGAAGCTTTAGAGACAAGAGTACATCAAAGAATTCAGACATGAAAAAAATATCAGATTCTAGGAAGTGTTTGGGCAGGAATGCTTCTGATGTTTTGTTTCTGGAAAGAGAGGATAATGATGAGTGCTTCCAGGATTGCACAGTTGGAAGTAAGGATATCTCTGATCTAGTAAAAAAAGCAGTTAGAGCTGCAGAAGCTGAAGCTAGAGCAGCCAATGCACCTGTAGAAGCCATCAAAGCTGCTGGTGATGCTGCTGCGGAAGTTGTCAAAAGTGCAGCTTTAGAGGAATTCACAACTACAAACAACGAAGAAGCTGCAGTTTTGGCTGCTTCAAGAGCAGCATCTACAGTAATTGATGCCGCCAATTCAATTGAAGCTCTAAGTGCGAATGCAGTAAATACCTCTGCTAGTGCTGAACCAATAACTTCAAGCTCTGAACCTCAAAACCATGAAGATGTTGAAGAATACTTTATTCCAAGTGTTGAATCCCTTGCACAGTTGAGAGAAAAATACTGTATTCAATGTCTTGAGACTCTTGGAGAATATGTTGAAGTTCTTGGACCTGTATTGCATGAGAAGGGAGTAGACGTATGTCTTGCCCTGTTGCAACGAAATTCAAGACATAAAGAACCGTCAAAGGTCGCAATGCTTTTGCCTGATGTAATGAAGCTCATATGTGCTCTGGCTGCTCACCGGAAATTTGCTGCATTGTTCGTGGATAGGGGTGGCATGCAGAAACTGCTTGCTGTCCCTAGAGTTGCTCAAACCTACTTTGGCCTTTCTTCTTGCTTATTTACAATCGGTTCGCTGCAGGGTATAATGGAGCGTGTATGTGCTCTTCCCTCAGACTTGGTTTACCAGGTGGTTGAGTTGGCTCTCCATCTTCTCGAGTGCTCACAGGATCAAGCCAGGAAAAATGCAGCCTTATTTTTTTCTGCTGCATTTGTTTTCAAGGCAGTTCTTGATGCTTTTGATGCTCAGGATGGCTTACAGAAAATACTGGGCCTTTTAAATGATGCTGCATCAGTAAGATCTGGAGTAAATTCTGGGACATTAGGACTCTCCACCTCAGGCTCACTTCGAAATGATAGGTCACCGACAGAAGTTCTGACCTCATCAGAGAAACAGATCGCTTATCACACCTGTGTTGCTTTGCGTCAGTACTTTAGAGCTCATTTGATTTTGCTTGTGGATTCTCTTCGTCCAAATAAAAACAGTCGAAGTGCAGCTCGAAATCTTCCAAGTGTAAGGGCAGCTTACAAGCCACTTGACCTTAGCAACGAAGCTATTGATGCAGTATTTCTGCAGTTACAGAAGGACCGGAAACTTGGTCCTGCATTTGTAAGAACTCGTTGGCCTGCAGTTGATAGGTTTTTGGGATATAATGGACATATTACTATGTTGGAGTTATGCCAGGCCCCACCTGTTGAGCGCTATCTACATGATTTACTTCAATATGCATTGGGTGTTCTGCACATTGTTACATTGGTACCTAGCAGTCGCAAGATGATTGTGAATTCAACATTAAGCAACAATCGTGTTGGTATAGCAGTTATATTGGATGCAGCAAGTGTTAATGGTAGTTATGTGGACCCCGAGATCATTCAGCCAGCTCTAAATGTGTTAGTCAATCTCGTTTGTCCTCCACCTTCAATCAGCAACAAACCACCTTTACTTGCACAAAGTCAGCAGTCTGTTTCTGCCCCAACCTCAAATGCCCTTGCTATAGAGACTAGAGAGAAAAATACTGAACGTAATGTCTCAGATCGAGCTGGGGAATCAGCTGTTGTAGATCGAGGCAGTGTAGCAGCACTGGGTACTGGTACACAGTCAAACAGTAGCAATGCACTATCCTCTACCCCCACAGCAACTTCTGGATTGGTGGGGGATCGCAGGATATCTTTAGGAGTTGGGGCAGGTTGTGCTGGGCTTGCGGCACAACTGGAGCAAGGCTATCGGCAAGCAAGAGAGGCTGTTCGCTCCACCAACGGTATAAAGGTTCTGCTGCATCTTCTCCAACCACGCATATATTCACCTCCTGCCGCACTCGATTGTCTACGTGCACTTGCCTGCCGAGTCCTTCTTGGTTTAGCGAGAGATGATACAATTGCACACATATTGACAAAGCTCCAGGTTGGAAAGAAACTATCAGAACTAATTCGAGATCAAACACAAGGAGCTGAGCAAGGCAGGTGGCAATCTGAACTTTCACAGGCAGCAATCGAGTTAATGGCGATTGTGACGAATTCTGGACGTGCTAGCACATTGGCTGCCACTGATGCCGCTATGCCAACTTTGAGGCGCATAGAACGGGCAGCTATAGCTGCTGCCACTCCTATCACTTACCATTCCAGGGAGCTTTTGCTTCTAATACATGAACACCTACAGGCATCTGGTTTGGCCACAACAGCTGCTTCACTGCTAAAAGAGGCCCAGTTGACGTCTTTGCCATCCTTGGCTGCCCCCTCATCTCTTGTTCATCAAGCTACACAAGAAGCCTCATCTCTGCAGCTTCAGTGGCCCTCTGGTCGCACCCCTATTGGATTTCTTACCAACAAATCAAAGATAGCAAGGGAGGAGGATTCAAGCTTGAAATGTGATTCTACTGTCTCATACTCAAAGAAAAAACCTTTGGTTTTCTCTCCAAGTTTTTGTCTTCAGTCAAGGAATCCGTTCCAGTCCCATGACTCTCACCCAACATTGGCCACAAATGTCTTAAGTGCATCGAAAGAGTTATCTGCACCTGCAAATGCATCAGAAGCTCCATCAGATATCCTGCCGAAACCTAGTATTGATACAGAGTACCAGTGTAAGACACCCATCCTGTTGCCGATGAAAAGAAAGTTATCCGAGTTAAAGGATCCTGGATGTTTGCCATCATCTGGGAAACGCATCCACACTGGGGACCAGGGATATCGGTCTCCAATTTTTCCCACACCCAATATTGTGCGGAAAAGCGGTCTACTAACTGATCTGGCTGGGTTCTCTACTCCTACTTCCAACATGAGAGATCAGCATTGTCGATCCACACCTGCTTGTTTTTCATCAGAGTACCTGGATGATAACCAGTATGGTAATTCTAGTGTAGGTCTGGCAACACCATCCTCCCAACTTGGGCTTCAAAACGACCCTCAACCAAGCAACCCAGAAAGATTGACTCTGGACTCTTTGGTTGTTCAGTATCTGAAGCACCAGCATCGCCAGTGTCCTGCCCCTATAACCACTCTTCCACCACTGTCCCTGTTACAGCCACATGTTTGTCCTGAACCAAGACGAACCCTTGATGCCCCTTCAAATGTAACTGCCCGGCTTGGCACCCGTGAGTTCAGAAGTATGTATGGTGGTGTGCATGGAAACCGCAGGGATCGTCAGTTTGTTTACAGTAGATTCCGGCCATGGCGGACTTGTAGGGATGATACTGGTACCCCTTTGACATGCATCTCTTTTCTGAGTGACACTGCTCGTATTGCAGTAGGCAGTCATGGTGGAGAGCTCAAAATTTTTGATTCCAATAGCAGCAATGTATTGGAGAGTTGTCCAAGCCACCAGTCTCCTGTAACGCTTGTTCAGACATATCATTCAGGTGAAACCGAATTGGTGCTGTCTTCTAGTTCAGAAGATGTGAGGTTGTGGGATGCATCTACAGTCTCATCTGGGCCAATGCATTCATATGAAGGGTGCAAGGCTGCAAGATTTAGCAATTCTGGGGATGTTTTTGCAGCCCTATCATCAGAGCTGGCGCAAAAAGAAATTCTAATATATGACATCCAAACTAACCGGTTGGAATCAAAACTGTCAGATACAGCTGCTAGCACGGGACGTGGACATTCATATTCTCATATACACTTCAACCCTTCAGACACAATGCTGCTCTGGAATGGTGTCCTATGGGACAGGCGGGTTTCCAGCCCTGTTCATCGTTTTGATCAGTTTACGGATTATGGGGGTGGTGGCTTCCATCCTGCTGGGAACGAGGTGATTATAAACTCTGAGGTCTGGGATCTCCGGAACTTTAGACTCCTCCGAAGTGTACCTTCTTTAGACCAAACTACAATAACTTTTAATGCCCGTGGTGATGTAATTTATGCAATCCTCCGGAGAAATCTTGATGATGTAATGTCGGCGGTGAATACCCGTCGCGTAAAGCATCCTCTCTTTGCTGCTTTCCGCACAGTGGATGCGGTTAACTACTCGGACATTGCCACGATTCCGGTTGATCGTTGTGTCCTCGACTTTGCAACAGAGCCAACTGATTCATTTCTTGGGCTGATCACAATGGATGACCAAGATGAGATGTTTGCTTCTGCTAGGGTTTATGAAATTGGTCGCCGAAAGCCGACAGATGATGATTCTGATCCTGATGATGCCGAGAGTGAAGAGGATGATGATGATGAAGAAGACGACGACGATGCGGATATGGACACTCTACTGGGCCCTGATCTTGACGGGGATGATGATAGTGATATGAGTAATGATGATGATGATGATAGTGGGAGCGACCTTGATGACGACGATGAGGGAGATTATATGATGGATGGGGACTTTGATGGAGGAGGTGGAATGCTTGAGATTTTGACAGACGGTGAGGAAGATGACGATGACAGTGATGGACTGGTTGAATCCTTTAGCAGTGGTGATGAGGAACATTTTGCAGGCCATGGTTTCGGTTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

1953

Amino Acids

212.74

Weight (kDa)

4.97

Isoelectric Point (pI)

48.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Beta-prop_WDR3_2nd PF25172 1538 - 1613 8.8e-07 WDR3 second beta-propeller domain
Beta-prop_WDR3_1st PF25173 1538 - 1611 1.5e-06 WDR3 first beta-propeller domain
Beta-prop_WDR5 PF25175 1539 - 1663 2.6e-08 WDR5 beta-propeller domain
Beta-prop_THOC3 PF25174 1541 - 1686 1.6e-07 THOC3 beta-propeller domain
WD40_Prp19 PF24814 1541 - 1708 5.9e-06 Prp19 WD40 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 5171
AarI CACCTGC 3 cut(s) 3369, 3940, 4228
AatII GACGTC 2 cut(s) 659, 3632
Acc36I ACCTGC 5 cut(s) 796, 3095, 3369, 3940, 4228
Acc65I GGTACC 2 cut(s) 2677, 4610
AccB1I GGYRCC 4 cut(s) 67, 2677, 4502, 4610
AccB7I CCANNNNNTGG 2 cut(s) 953, 4070
AccI GTMKAC 3 cut(s) 1773, 3244, 4150
AccII CGCG 2 cut(s) 41, 5328
AccIII TCCGGA 3 cut(s) 659, 5193, 5283
AclI AACGTT 1 cut(s) 925
AcoI YGGCCR 4 cut(s) 147, 3588, 3891, 4580
AcuI CTGAAG 6 cut(s) 605, 1407, 3686, 3830, 4394, 5028
AcyI GRCGYC 3 cut(s) 68, 656, 3629
AfaI GTAC 9 cut(s) 1210, 2351, 2679, 3007, 3013, 3992, 4241, 4612, 5220
AfeI AGCGCT 1 cut(s) 2621
AflII CTTAAG 1 cut(s) 3904
AflIII ACRYGT 1 cut(s) 4441
AgeI ACCGGT 1 cut(s) 4965
AjiI CACGTC 2 cut(s) 3443, 5009
AjuI GAANNNNNNNTTGG 6 cut(s) 533, 565, 3803, 3835, 4290, 4322
AleI CACNNNNGTG 1 cut(s) 2331
Alw21I GWGCWC 6 cut(s) 1870, 2026, 2084, 2362, 3253, 4683
Alw44I GTGCAC 1 cut(s) 3249
AlwNI CAGNNNCTG 5 cut(s) 410, 811, 1385, 1927, 4372
Ama87I CYCGRG 2 cut(s) 2075, 2783
Aor13HI TCCGGA 3 cut(s) 659, 5193, 5283
Aor51HI AGCGCT 1 cut(s) 2621
ApaI GGGCCC 1 cut(s) 5624
ApaLI GTGCAC 1 cut(s) 3249
AseI ATTAAT 1 cut(s) 353
AsiGI ACCGGT 1 cut(s) 4965
Asp700I GAANNNNTTC 2 cut(s) 3345, 3860
Asp718I GGTACC 2 cut(s) 2677, 4610
AspLEI GCGC 6 cut(s) 43, 70, 1074, 2622, 3492, 4930
AsuC2I CCSGG 3 cut(s) 65, 1161, 4495
AsuII TTCGAA 1 cut(s) 2269
AsuNHI GCTAGC 2 cut(s) 3446, 4997
AvaI CYCGRG 2 cut(s) 2075, 2783
AvaII GGWCC 5 cut(s) 1748, 2506, 2519, 2779, 4093
AxyI CCTNAGG 1 cut(s) 2257
BaeGI GKGCMC 3 cut(s) 1096, 3253, 5624
BaeI ACNNNNGTAYC 4 cut(s) 880, 913, 2661, 2694
BalI TGGCCA 3 cut(s) 149, 3590, 3893
BamHI GGATCC 1 cut(s) 4048
BanI GGYRCC 4 cut(s) 67, 2677, 4502, 4610
BanII GRGCYC 3 cut(s) 2362, 4683, 5624
BarI GAAGNNNNNNTAC 2 cut(s) 2010, 2042
BauI CACGAG 1 cut(s) 1002
BbsI GAAGAC 8 cut(s) 108, 117, 138, 474, 593, 3836, 4785, 5592
Bbv12I GWGCWC 6 cut(s) 1870, 2026, 2084, 2362, 3253, 4683
BceAI ACGGC 1 cut(s) 326
BcgI CGANNNNNNTGC 8 cut(s) 1484, 1518, 3125, 3159, 4367, 4401, 5579, 5613
BclI TGATCA 2 cut(s) 5113, 5457
BcnI CCSGG 3 cut(s) 65, 1161, 4495
BfoI RGCGCY 2 cut(s) 71, 2623
BfrI CTTAAG 1 cut(s) 3904
BfuAI ACCTGC 5 cut(s) 796, 3095, 3369, 3940, 4228
BglII AGATCT 1 cut(s) 2219
BlpI GCTNAGC 1 cut(s) 3369
BmcAI AGTACT 1 cut(s) 2351
Bme18I GGWCC 5 cut(s) 1748, 2506, 2519, 2779, 4093
BmeT110I CYCGRG 2 cut(s) 2075, 2783
BmgBI CACGTC 2 cut(s) 3443, 5009
BmrI ACTGGG 7 cut(s) 502, 633, 2198, 3011, 3616, 4098, 5627
BmtI GCTAGC 2 cut(s) 3450, 5001
BmuI ACTGGG 7 cut(s) 502, 633, 2198, 3011, 3616, 4098, 5627
BoxI GACNNNNGTC 1 cut(s) 1779
BpiI GAAGAC 8 cut(s) 108, 117, 138, 474, 593, 3836, 4785, 5592
BpmI CTGGAG 3 cut(s) 2244, 3152, 3302
Bpu10I CCTNAGC 2 cut(s) 2166, 2462
Bpu1102I GCTNAGC 1 cut(s) 3369
Bpu14I TTCGAA 1 cut(s) 2269
BpuEI CTTGAG 3 cut(s) 1051, 1736, 5774
BpuMI CCSGG 3 cut(s) 65, 1161, 4495
BsaAI YACGTR 2 cut(s) 1013, 3248
BsaBI GATNNNNATC 1 cut(s) 5541
BsaHI GRCGYC 3 cut(s) 68, 656, 3629
BsaI GGTCTC 2 cut(s) 571, 4113
BsaWI WCCGGW 7 cut(s) 659, 1881, 2508, 4965, 5193, 5283, 5398
BsaXI ACNNNNNCTCC 4 cut(s) 1719, 1749, 1761, 1791
Bse118I RCCGGY 1 cut(s) 4965
Bse21I CCTNAGG 1 cut(s) 2257
Bse3DI GCAATG 5 cut(s) 289, 1839, 3037, 4714, 5385
Bse8I GATNNNNATC 1 cut(s) 5541
BseAI TCCGGA 3 cut(s) 659, 5193, 5283
BseJI GATNNNNATC 1 cut(s) 5541
BseMI GCAATG 5 cut(s) 289, 1839, 3037, 4714, 5385
BseRI GAGGAG 8 cut(s) 104, 131, 275, 933, 1054, 3779, 5198, 5754
BseSI GKGCMC 3 cut(s) 1096, 3253, 5624
BseYI CCCAGC 5 cut(s) 197, 2960, 3113, 4166, 5153
BsgI GTGCAG 4 cut(s) 1482, 2433, 2645, 3912
Bsh1236I CGCG 2 cut(s) 41, 5328
BshNI GGYRCC 4 cut(s) 67, 2677, 4502, 4610
BshTI ACCGGT 1 cut(s) 4965
BsiHKAI GWGCWC 6 cut(s) 1870, 2026, 2084, 2362, 3253, 4683
BsiHKCI CYCGRG 2 cut(s) 2075, 2783
BslFI GGGAC 9 cut(s) 1194, 1922, 2251, 3853, 4106, 4411, 5019, 5093, 5741
BsmFI GGGAC 9 cut(s) 1194, 1922, 2251, 3853, 4106, 4411, 5019, 5093, 5741
BsmI GAATGC 4 cut(s) 1273, 1582, 4852, 5754
Bso31I GGTCTC 2 cut(s) 571, 4113
BsoBI CYCGRG 2 cut(s) 2075, 2783
Bsp119I TTCGAA 1 cut(s) 2269
Bsp120I GGGCCC 1 cut(s) 5620
Bsp1286I GDGCHC 8 cut(s) 1096, 1870, 2026, 2084, 2362, 3253, 4683, 5624
Bsp13I TCCGGA 3 cut(s) 659, 5193, 5283
Bsp1720I GCTNAGC 1 cut(s) 3369
Bsp19I CCATGG 2 cut(s) 4583, 5845
BspEI TCCGGA 3 cut(s) 659, 5193, 5283
BspFNI CGCG 2 cut(s) 41, 5328
BspMAI CTGCAG 6 cut(s) 1381, 1507, 2001, 2496, 2551, 3699
BspMI ACCTGC 5 cut(s) 796, 3095, 3369, 3940, 4228
BspOI GCTAGC 2 cut(s) 3450, 5001
BspQI GCTCTTC 1 cut(s) 2031
BspT104I TTCGAA 1 cut(s) 2269
BspT107I GGYRCC 4 cut(s) 67, 2677, 4502, 4610
BspTI CTTAAG 1 cut(s) 3904
BspTNI GGTCTC 2 cut(s) 571, 4113
BsrDI GCAATG 5 cut(s) 289, 1839, 3037, 4714, 5385
BsrFI RCCGGY 1 cut(s) 4965
BssAI RCCGGY 1 cut(s) 4965
BssNI GRCGYC 3 cut(s) 68, 656, 3629
BssSI CACGAG 1 cut(s) 1002
BssT1I CCWWGG 6 cut(s) 28, 150, 836, 3642, 4583, 5845
Bst2BI CACGAG 1 cut(s) 1002
Bst6I CTCTTC 4 cut(s) 2031, 2391, 4419, 5559
BstACI GRCGYC 3 cut(s) 68, 656, 3629
BstAFI CTTAAG 1 cut(s) 3904
BstAPI GCANNNNNTGC 4 cut(s) 410, 1403, 1927, 3110
BstBAI YACGTR 2 cut(s) 1013, 3248
BstBI TTCGAA 1 cut(s) 2269
BstDSI CCRYGG 4 cut(s) 1084, 4583, 5257, 5845
BstEII GGTNACC 1 cut(s) 2280
BstFNI CGCG 2 cut(s) 41, 5328
BstH2I RGCGCY 2 cut(s) 71, 2623
BstHHI GCGC 6 cut(s) 43, 70, 1074, 2622, 3492, 4930
BstNSI RCATGY 3 cut(s) 1921, 4445, 4626
BstPAI GACNNNNGTC 1 cut(s) 1779
BstPI GGTNACC 1 cut(s) 2280
BstSLI GKGCMC 3 cut(s) 1096, 3253, 5624
BstUI CGCG 2 cut(s) 41, 5328
BstV2I GAAGAC 8 cut(s) 108, 117, 138, 474, 593, 3836, 4785, 5592
BstX2I RGATCY 4 cut(s) 1163, 2219, 4048, 5188
BstXI CCANNNNNNTGG 1 cut(s) 5153
BstYI RGATCY 4 cut(s) 1163, 2219, 4048, 5188
Bsu36I CCTNAGG 1 cut(s) 2257
BtgI CCRYGG 4 cut(s) 1084, 4583, 5257, 5845
BtgZI GCGATG 1 cut(s) 4370
BtrI CACGTC 2 cut(s) 3443, 5009
BtsI GCAGTG 5 cut(s) 1000, 2995, 3605, 4644, 5824
BveI ACCTGC 5 cut(s) 796, 3095, 3369, 3940, 4228
CaiI CAGNNNCTG 5 cut(s) 410, 811, 1385, 1927, 4372
CfoI GCGC 6 cut(s) 43, 70, 1074, 2622, 3492, 4930
Cfr10I RCCGGY 1 cut(s) 4965
CseI GACGC 2 cut(s) 381, 2333
CsiI ACCWGGT 1 cut(s) 2046
Csp6I GTAC 9 cut(s) 1209, 2350, 2678, 3006, 3012, 3991, 4240, 4611, 5219
CspAI ACCGGT 1 cut(s) 4965
CspCI CAANNNNNGTGG 2 cut(s) 3362, 3397
CviQI GTAC 9 cut(s) 1209, 2350, 2678, 3006, 3012, 3991, 4240, 4611, 5219
DinI GGCGCC 1 cut(s) 69
DraI TTTAAA 1 cut(s) 2199
EaeI YGGCCR 4 cut(s) 147, 3588, 3891, 4580
Eam1104I CTCTTC 4 cut(s) 2031, 2391, 4419, 5559
EarI CTCTTC 4 cut(s) 2031, 2391, 4419, 5559
EciI GGCGGA 2 cut(s) 42, 4603
Ecl136II GAGCTC 2 cut(s) 2360, 4681
Eco130I CCWWGG 6 cut(s) 28, 150, 836, 3642, 4583, 5845
Eco24I GRGCYC 3 cut(s) 2362, 4683, 5624
Eco31I GGTCTC 2 cut(s) 571, 4113
Eco32I GATATC 4 cut(s) 1345, 3087, 3961, 4103
Eco47I GGWCC 5 cut(s) 1748, 2506, 2519, 2779, 4093
Eco47III AGCGCT 1 cut(s) 2621
Eco53kI GAGCTC 2 cut(s) 2360, 4681
Eco57I CTGAAG 6 cut(s) 605, 1407, 3686, 3830, 4394, 5028
Eco81I CCTNAGG 1 cut(s) 2257
Eco88I CYCGRG 2 cut(s) 2075, 2783
Eco91I GGTNACC 1 cut(s) 2280
EcoICRI GAGCTC 2 cut(s) 2360, 4681
EcoO109I RGGNCCY 2 cut(s) 2605, 5621
EcoO65I GGTNACC 1 cut(s) 2280
EcoRI GAATTC 4 cut(s) 1219, 1475, 2704, 3433
EcoRV GATATC 4 cut(s) 1345, 3087, 3961, 4103
EcoT14I CCWWGG 6 cut(s) 28, 150, 836, 3642, 4583, 5845
EcoT22I ATGCAT 5 cut(s) 2650, 3943, 4628, 4828, 4854
EcoT38I GRGCYC 3 cut(s) 2362, 4683, 5624
EgeI GGCGCC 1 cut(s) 69
EheI GGCGCC 1 cut(s) 69
ErhI CCWWGG 6 cut(s) 28, 150, 836, 3642, 4583, 5845
FalI AAGNNNNNCTT 6 cut(s) 1450, 1482, 2428, 2460, 5206, 5238
FaqI GGGAC 9 cut(s) 1194, 1922, 2251, 3853, 4106, 4411, 5019, 5093, 5741
FauI CCCGC 1 cut(s) 5079
FauNDI CATATG 2 cut(s) 1862, 4858
FbaI TGATCA 2 cut(s) 5113, 5457
FblI GTMKAC 3 cut(s) 1773, 3244, 4150
FriOI GRGCYC 3 cut(s) 2362, 4683, 5624
GlaI GCGC 6 cut(s) 42, 69, 1073, 2621, 3491, 4929
GsaI CCCAGC 5 cut(s) 201, 2964, 3117, 4170, 5157
GsuI CTGGAG 3 cut(s) 2244, 3152, 3302
HaeII RGCGCY 2 cut(s) 71, 2623
HgaI GACGC 2 cut(s) 381, 2333
HhaI GCGC 6 cut(s) 43, 70, 1074, 2622, 3492, 4930
Hin1I GRCGYC 3 cut(s) 68, 656, 3629
Hin6I GCGC 6 cut(s) 41, 68, 1072, 2620, 3490, 4928
HinP1I GCGC 6 cut(s) 41, 68, 1072, 2620, 3490, 4928
HincII GTYRAC 4 cut(s) 654, 1152, 3627, 5374
HindII GTYRAC 4 cut(s) 654, 1152, 3627, 5374
HindIII AAGCTT 2 cut(s) 1192, 3775
HpaI GTTAAC 1 cut(s) 5374
Hpy99I CGWCG 6 cut(s) 11, 110, 5328, 5594, 5597, 5702
Hsp92I GRCGYC 3 cut(s) 68, 656, 3629
HspAI GCGC 6 cut(s) 41, 68, 1072, 2620, 3490, 4928
KasI GGCGCC 1 cut(s) 67
Kpn2I TCCGGA 3 cut(s) 659, 5193, 5283
KpnI GGTACC 2 cut(s) 2681, 4614
Ksp22I TGATCA 2 cut(s) 5113, 5457
KspAI GTTAAC 1 cut(s) 5374
LguI GCTCTTC 1 cut(s) 2031
MabI ACCWGGT 1 cut(s) 2046
MaeIII GTNAC 9 cut(s) 841, 2280, 2496, 2668, 3427, 4431, 4486, 4640, 4744
MfeI CAATTG 2 cut(s) 1557, 3294
MflI RGATCY 4 cut(s) 1163, 2219, 4048, 5188
MhlI GDGCHC 8 cut(s) 1096, 1870, 2026, 2084, 2362, 3253, 4683, 5624
MlsI TGGCCA 3 cut(s) 149, 3590, 3893
MluNI TGGCCA 3 cut(s) 149, 3590, 3893
Mly113I GGCGCC 1 cut(s) 68
MlyI GAGTC 9 cut(s) 719, 1055, 1714, 2241, 3273, 3869, 4339, 4346, 5199
MmeI TCCRAC 6 cut(s) 1312, 2571, 3229, 3304, 4212, 4950
Mox20I TGGCCA 3 cut(s) 149, 3590, 3893
Mph1103I ATGCAT 5 cut(s) 2650, 3943, 4628, 4828, 4854
MroI TCCGGA 3 cut(s) 659, 5193, 5283
MroXI GAANNNNTTC 2 cut(s) 3345, 3860
MscI TGGCCA 3 cut(s) 149, 3590, 3893
MslI CAYNNNNRTG 5 cut(s) 828, 1641, 2331, 4770, 4857
Msp20I TGGCCA 3 cut(s) 149, 3590, 3893
MspA1I CMGCKG 5 cut(s) 407, 1147, 2972, 3599, 4994
MspCI CTTAAG 1 cut(s) 3904
MunI CAATTG 2 cut(s) 1557, 3294
Mva1269I GAATGC 4 cut(s) 1273, 1582, 4852, 5754
MvnI CGCG 2 cut(s) 41, 5328
NarI GGCGCC 1 cut(s) 68
NciI CCSGG 3 cut(s) 65, 1161, 4495
NcoI CCATGG 2 cut(s) 4583, 5845
NdeI CATATG 2 cut(s) 1862, 4858
NheI GCTAGC 2 cut(s) 3446, 4997
NmeAIII GCCGAG 2 cut(s) 3287, 5581
NmuCI GTSAC 3 cut(s) 2280, 3427, 4640
NsiI ATGCAT 5 cut(s) 2650, 3943, 4628, 4828, 4854
NspI RCATGY 3 cut(s) 1921, 4445, 4626
NspV TTCGAA 1 cut(s) 2269
OliI CACNNNNGTG 1 cut(s) 2331
PaeI GCATGC 1 cut(s) 1921
PaeR7I CTCGAG 1 cut(s) 2075
PaqCI CACCTGC 3 cut(s) 3369, 3940, 4228
PasI CCCWGGG 1 cut(s) 22
PciI ACATGT 1 cut(s) 4441
PciSI GCTCTTC 1 cut(s) 2031
PcsI WCGNNNNNNNCGW 2 cut(s) 111, 1009
PctI GAATGC 4 cut(s) 1273, 1582, 4852, 5754
PdmI GAANNNNTTC 2 cut(s) 3345, 3860
PflMI CCANNNNNTGG 2 cut(s) 953, 4070
PfoI TCCNGGA 2 cut(s) 1317, 4051
PinAI ACCGGT 1 cut(s) 4965
PleI GAGTC 9 cut(s) 718, 1055, 1714, 2241, 3272, 3869, 4339, 4346, 5199
PluTI GGCGCC 1 cut(s) 71
PpsI GAGTC 9 cut(s) 718, 1055, 1714, 2241, 3272, 3869, 4339, 4346, 5199
Ppu21I YACGTR 2 cut(s) 1013, 3248
PscI ACATGT 1 cut(s) 4441
PshAI GACNNNNGTC 1 cut(s) 1779
PshBI ATTAAT 1 cut(s) 353
PsiI TTATAA 1 cut(s) 5171
Psp124BI GAGCTC 2 cut(s) 2362, 4683
Psp1406I AACGTT 1 cut(s) 925
PspEI GGTNACC 1 cut(s) 2280
PspFI CCCAGC 5 cut(s) 197, 2960, 3113, 4166, 5153
PspOMI GGGCCC 1 cut(s) 5620
PsrI GAACNNNNNNTAC 2 cut(s) 4737, 4769
PstI CTGCAG 6 cut(s) 1381, 1507, 2001, 2496, 2551, 3699
PstNI CAGNNNCTG 5 cut(s) 410, 811, 1385, 1927, 4372
PsuI RGATCY 4 cut(s) 1163, 2219, 4048, 5188
PvuII CAGCTG 5 cut(s) 407, 1147, 2972, 3599, 4994
RsaI GTAC 9 cut(s) 1210, 2351, 2679, 3007, 3013, 3992, 4241, 4612, 5220
RsaNI GTAC 9 cut(s) 1209, 2350, 2678, 3006, 3012, 3991, 4240, 4611, 5219
RseI CAYNNNNRTG 5 cut(s) 828, 1641, 2331, 4770, 4857
SacI GAGCTC 2 cut(s) 2362, 4683
SapI GCTCTTC 1 cut(s) 2031
ScaI AGTACT 1 cut(s) 2351
SchI GAGTC 9 cut(s) 719, 1055, 1714, 2241, 3273, 3869, 4339, 4346, 5199
SduI GDGCHC 8 cut(s) 1096, 1870, 2026, 2084, 2362, 3253, 4683, 5624
SexAI ACCWGGT 1 cut(s) 2046
SfoI GGCGCC 1 cut(s) 69
Sfr274I CTCGAG 1 cut(s) 2075
SfuI TTCGAA 1 cut(s) 2269
SinI GGWCC 5 cut(s) 1748, 2506, 2519, 2779, 4093
SlaI CTCGAG 1 cut(s) 2075
SmiMI CAYNNNNRTG 5 cut(s) 828, 1641, 2331, 4770, 4857
SmlI CTYRAG 5 cut(s) 1066, 1715, 2075, 3904, 5753
SmoI CTYRAG 5 cut(s) 1066, 1715, 2075, 3904, 5753
SphI GCATGC 1 cut(s) 1921
SspDI GGCGCC 1 cut(s) 67
SspI AATATT 1 cut(s) 4132
SstI GAGCTC 2 cut(s) 2362, 4683
StyI CCWWGG 6 cut(s) 28, 150, 836, 3642, 4583, 5845
TaqII GACCGA 1 cut(s) 4095
TatI WGTACW 2 cut(s) 1208, 2349
TauI GCSGC 6 cut(s) 41, 58, 1550, 3125, 3233, 3473
TseFI GTSAC 3 cut(s) 2280, 3427, 4640
Tsp45I GTSAC 3 cut(s) 2280, 3427, 4640
TspGWI ACGGA 4 cut(s) 385, 686, 3849, 5138
Van91I CCANNNNNTGG 2 cut(s) 953, 4070
Vha464I CTTAAG 1 cut(s) 3904
VneI GTGCAC 1 cut(s) 3249
VpaK11BI GGWCC 5 cut(s) 1748, 2506, 2519, 2779, 4093
VspI ATTAAT 1 cut(s) 353
XbaI TCTAGA 1 cut(s) 1100
XceI RCATGY 3 cut(s) 1921, 4445, 4626
XhoI CTCGAG 1 cut(s) 2075
XmiI GTMKAC 3 cut(s) 1773, 3244, 4150
XmnI GAANNNNTTC 2 cut(s) 3345, 3860
ZraI GACGTC 2 cut(s) 657, 3630
ZrmI AGTACT 1 cut(s) 2351
Zsp2I ATGCAT 5 cut(s) 2650, 3943, 4628, 4828, 4854
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.