Rorug06G0509000

rRNA binding

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000006
OG0002783
Physical Location & Seq
Reverse (-)
62351818 .. 62354995
3178 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug06G0509000.1

Sequence Viewer

Length: 588 bp
ATGGCATGTGGGACTCTTGTAGTTTTGGACATTCATACCCTCCAAGTTGTTTATGAAGATTACTCAGTTCTCACCCTCAGTGTCCCTTATGTTCCTGGCTTCCTTGCATTCAGAGAGGCCCCGGTGCTTCTTGAGCTTTTGGAGAAGATGAAAGCCGATGCTAATCTTTTCTATCCGCAGCTGCTAATGGTAGATGGAAACGGAATACTTCATCCTCGAGGTTTTGGCTTGGCTTGTCATCTTGGCATTCTAGCAAATCTACCTACTATTGGGATTGCAAAAAATCTTCATCATGTGGATGGTCTAACATTATCTGGTGTGAGGCAACTTCTTAAAGCCAAAGAAGACTGTGCTGAAGATTTTATTAATTTGACAGGACGCTCAGGGCGTATATGGGGAGTGGCAATGAGATCTACAGGCGGCTGCGTGAAGCCTATATTTATTTCAATTGGTCACCGCATTTCGCTTGATACTGCCATCAAAATTGTAAAACTGACTTGCAAGTATCGTGTGCCAGAGCCTATCCGGCAGGGTGATATCAGATCTAGAGAATATTTTCGGAAGCATCAAGCGATGATCTATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

195

Amino Acids

21.69

Weight (kDa)

9.13

Isoelectric Point (pI)

32.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Endonuclease_5 PF04493 3 - 179 1.8e-58 Endonuclease V
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AbsI CCTCGAGG 1 cut(s) 216
AciI CCGC 3 cut(s) 176, 420, 457
AcuI CTGAAG 1 cut(s) 375
AfiI CCNNNNNNNGG 1 cut(s) 269
AgsI TTSAA 1 cut(s) 447
AjnI CCWGG 1 cut(s) 94
AluBI AGCT 2 cut(s) 136, 181
AluI AGCT 2 cut(s) 136, 181
Ama87I CYCGRG 1 cut(s) 216
AoxI GGCC 1 cut(s) 117
ApeKI GCWGC 3 cut(s) 178, 181, 423
AseI ATTAAT 1 cut(s) 366
Asp700I GAANNNNTTC 1 cut(s) 555
AspS9I GGNCC 1 cut(s) 118
AsuC2I CCSGG 1 cut(s) 122
AsuHPI GGTGA 3 cut(s) 64, 446, 545
AvaI CYCGRG 1 cut(s) 216
BbsI GAAGAC 1 cut(s) 351
BbvI GCAGC 3 cut(s) 168, 190, 410
BccI CCATC 3 cut(s) 188, 293, 485
BciT130I CCWGG 1 cut(s) 96
BcnI CCSGG 1 cut(s) 122
BfaI CTAG 2 cut(s) 251, 546
BfmI CTRYAG 1 cut(s) 414
BglI GCCNNNNNGGC 1 cut(s) 526
BglII AGATCT 2 cut(s) 410, 542
BisI GCNGC 4 cut(s) 179, 182, 421, 424
BlsI GCNGC 4 cut(s) 180, 183, 422, 425
Bme1390I CCNGG 2 cut(s) 96, 122
BmeT110I CYCGRG 1 cut(s) 216
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 1 cut(s) 120
BmrFI CCNGG 2 cut(s) 96, 122
BmsI GCATC 2 cut(s) 148, 574
BpiI GAAGAC 1 cut(s) 351
Bpu10I CCTNAGC 1 cut(s) 382
BpuEI CTTGAG 1 cut(s) 152
BpuMI CCSGG 1 cut(s) 122
BsaBI GATNNNNATC 1 cut(s) 162
BsaJI CCNNGG 1 cut(s) 120
Bsc4I CCNNNNNNNGG 1 cut(s) 269
Bse3DI GCAATG 1 cut(s) 411
Bse8I GATNNNNATC 1 cut(s) 162
BseBI CCWGG 1 cut(s) 96
BseDI CCNNGG 1 cut(s) 120
BseGI GGATG 2 cut(s) 211, 304
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 269
BseMI GCAATG 1 cut(s) 411
BseMII CTCAG 3 cut(s) 78, 91, 396
BseXI GCAGC 3 cut(s) 168, 190, 410
BshFI GGCC 1 cut(s) 119
BsiHKCI CYCGRG 1 cut(s) 216
BsiSI CCGG 2 cut(s) 122, 526
BslFI GGGAC 2 cut(s) 25, 68
BslI CCNNNNNNNGG 1 cut(s) 269
BsmFI GGGAC 2 cut(s) 25, 68
BsmI GAATGC 2 cut(s) 107, 246
BsnI GGCC 1 cut(s) 119
BsoBI CYCGRG 1 cut(s) 216
Bsp143I GATC 3 cut(s) 410, 542, 576
BspACI CCGC 3 cut(s) 176, 420, 457
BspANI GGCC 1 cut(s) 119
BspCNI CTCAG 3 cut(s) 77, 90, 395
BspLI GGNNCC 1 cut(s) 120
BsrDI GCAATG 1 cut(s) 411
BssECI CCNNGG 1 cut(s) 120
BssMI GATC 3 cut(s) 410, 542, 576
Bst2UI CCWGG 1 cut(s) 96
Bst4CI ACNGT 1 cut(s) 350
BstDEI CTNAG 3 cut(s) 64, 77, 382
BstEII GGTNACC 1 cut(s) 452
BstF5I GGATG 2 cut(s) 211, 304
BstKTI GATC 3 cut(s) 413, 545, 579
BstMBI GATC 3 cut(s) 410, 542, 576
BstMWI GCNNNNNNNGC 2 cut(s) 133, 526
BstNI CCWGG 1 cut(s) 96
BstNSI RCATGY 1 cut(s) 9
BstPI GGTNACC 1 cut(s) 452
BstSCI CCNGG 2 cut(s) 94, 120
BstSFI CTRYAG 1 cut(s) 414
BstV1I GCAGC 3 cut(s) 168, 190, 410
BstV2I GAAGAC 1 cut(s) 351
BstX2I RGATCY 2 cut(s) 410, 542
BstYI RGATCY 2 cut(s) 410, 542
BsuRI GGCC 1 cut(s) 119
BtsCI GGATG 2 cut(s) 211, 304
BtsIMutI CAGTG 1 cut(s) 85
Cfr13I GGNCC 1 cut(s) 118
CseI GACGC 1 cut(s) 387
CviAII CATG 2 cut(s) 6, 293
DdeI CTNAG 3 cut(s) 64, 77, 382
DpnI GATC 3 cut(s) 412, 544, 578
DpnII GATC 3 cut(s) 410, 542, 576
Eco32I GATATC 1 cut(s) 538
Eco57I CTGAAG 1 cut(s) 375
Eco88I CYCGRG 1 cut(s) 216
Eco91I GGTNACC 1 cut(s) 452
EcoO109I RGGNCCY 1 cut(s) 118
EcoO65I GGTNACC 1 cut(s) 452
EcoRII CCWGG 1 cut(s) 94
EcoRV GATATC 1 cut(s) 538
FaeI CATG 2 cut(s) 9, 296
FaiI YATR 9 cut(s) 7, 36, 54, 90, 294, 392, 394, 437, 582
FaqI GGGAC 2 cut(s) 25, 68
FatI CATG 2 cut(s) 5, 292
Fnu4HI GCNGC 4 cut(s) 179, 182, 421, 424
FokI GGATG 2 cut(s) 198, 311
Fsp4HI GCNGC 4 cut(s) 179, 182, 421, 424
FspBI CTAG 2 cut(s) 251, 546
GluI GCNGC 4 cut(s) 179, 182, 421, 424
HaeIII GGCC 1 cut(s) 119
HapII CCGG 2 cut(s) 122, 526
HgaI GACGC 1 cut(s) 387
Hin1II CATG 2 cut(s) 9, 296
HinfI GANTC 1 cut(s) 13
HpaII CCGG 2 cut(s) 122, 526
HphI GGTGA 3 cut(s) 64, 446, 545
Hpy188I TCNGA 3 cut(s) 113, 542, 561
Hpy188III TCNNGA 2 cut(s) 131, 546
HpyCH4III ACNGT 1 cut(s) 350
HpyCH4V TGCA 3 cut(s) 107, 278, 501
HpyF10VI GCNNNNNNNGC 2 cut(s) 133, 526
HpyF3I CTNAG 3 cut(s) 64, 77, 382
Hsp92II CATG 2 cut(s) 9, 296
Kzo9I GATC 3 cut(s) 410, 542, 576
Lsp1109I GCAGC 3 cut(s) 168, 190, 410
LweI GCATC 2 cut(s) 148, 574
MaeI CTAG 2 cut(s) 251, 546
MaeIII GTNAC 1 cut(s) 452
MalI GATC 3 cut(s) 412, 544, 578
MboI GATC 3 cut(s) 410, 542, 576
MboII GAAGA 5 cut(s) 68, 157, 278, 356, 368
MfeI CAATTG 1 cut(s) 447
MflI RGATCY 2 cut(s) 410, 542
MluCI AATT 3 cut(s) 367, 447, 483
MlyI GAGTC 1 cut(s) 7
MnlI CCTC 6 cut(s) 50, 86, 109, 212, 225, 315
MroXI GAANNNNTTC 1 cut(s) 555
MseI TTAA 2 cut(s) 333, 366
MslI CAYNNNNRTG 1 cut(s) 297
MspA1I CMGCKG 1 cut(s) 181
MspI CCGG 2 cut(s) 122, 526
MspR9I CCNGG 2 cut(s) 96, 122
MunI CAATTG 1 cut(s) 447
Mva1269I GAATGC 2 cut(s) 107, 246
MvaI CCWGG 1 cut(s) 96
MwoI GCNNNNNNNGC 2 cut(s) 133, 526
NciI CCSGG 1 cut(s) 122
NdeII GATC 3 cut(s) 410, 542, 576
NlaIII CATG 2 cut(s) 9, 296
NlaIV GGNNCC 1 cut(s) 120
NmuCI GTSAC 1 cut(s) 452
NspI RCATGY 1 cut(s) 9
PaeR7I CTCGAG 1 cut(s) 216
PcsI WCGNNNNNNNCGW 1 cut(s) 385
PctI GAATGC 2 cut(s) 107, 246
PdmI GAANNNNTTC 1 cut(s) 555
PkrI GCNGC 4 cut(s) 180, 183, 422, 425
PleI GAGTC 1 cut(s) 7
PpsI GAGTC 1 cut(s) 7
PshBI ATTAAT 1 cut(s) 366
Psp6I CCWGG 1 cut(s) 94
PspEI GGTNACC 1 cut(s) 452
PspGI CCWGG 1 cut(s) 94
PspN4I GGNNCC 1 cut(s) 120
PspPI GGNCC 1 cut(s) 118
PspXI VCTCGAGB 1 cut(s) 216
PsuI RGATCY 2 cut(s) 410, 542
PvuII CAGCTG 1 cut(s) 181
RseI CAYNNNNRTG 1 cut(s) 297
SaqAI TTAA 2 cut(s) 333, 366
SatI GCNGC 4 cut(s) 179, 182, 421, 424
Sau3AI GATC 3 cut(s) 410, 542, 576
Sau96I GGNCC 1 cut(s) 118
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 96, 122
SetI ASST 4 cut(s) 138, 183, 223, 265
SfaNI GCATC 2 cut(s) 148, 574
SfcI CTRYAG 1 cut(s) 414
Sfr274I CTCGAG 1 cut(s) 216
SlaI CTCGAG 1 cut(s) 216
SmiMI CAYNNNNRTG 1 cut(s) 297
SmlI CTYRAG 2 cut(s) 131, 216
SmoI CTYRAG 2 cut(s) 131, 216
Sse9I AATT 3 cut(s) 367, 447, 483
SsiI CCGC 3 cut(s) 176, 420, 457
SspI AATATT 1 cut(s) 554
SspMI CTAG 2 cut(s) 251, 546
StyD4I CCNGG 2 cut(s) 94, 120
TaaI ACNGT 1 cut(s) 350
TaqI TCGA 1 cut(s) 217
TasI AATT 3 cut(s) 367, 447, 483
TauI GCSGC 1 cut(s) 423
Tru1I TTAA 2 cut(s) 333, 366
Tru9I TTAA 2 cut(s) 333, 366
TscAI CASTG 1 cut(s) 85
TseFI GTSAC 1 cut(s) 452
TseI GCWGC 3 cut(s) 178, 181, 423
Tsp45I GTSAC 1 cut(s) 452
TspDTI ATGAA 5 cut(s) 23, 69, 164, 200, 278
TspGWI ACGGA 1 cut(s) 216
TspRI CASTG 1 cut(s) 85
VspI ATTAAT 1 cut(s) 366
XbaI TCTAGA 1 cut(s) 545
XceI RCATGY 1 cut(s) 9
XhoI CTCGAG 1 cut(s) 216
XmnI GAANNNNTTC 1 cut(s) 555
XspI CTAG 2 cut(s) 251, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.