FvH4_5g07520

ribosomal protein S21

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0002783
Physical Location & Seq
Forward (+)
4390971 .. 4392400
1430 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g07520.t1

Sequence Viewer

Length: 606 bp
ATGATGCTTGATTTGGGCCCGGGTCGGCACCAGAGAAGCCATGGCCGCCACACTCTCCAAGCTCCTCTCCTCCCTCCTCCTCCTCCCGCCTCCACCGCCGCAGGAACTCCCTCCTCCGCCACGTGTCCCTCTCCCTCTTCCCAAGAGGGCCCCACCCGCTCTGGTGGCCAGCTGCTGGGCCCCATCAAATGTAGCCTTCCCTCGCTGGCCAACTCAAACCTGCTCTTCTTCAGCTCGGCCGGGTTCAACGCCCAGGTGACGGTCCAGGAAAACGAGCCGGAGGAGTGGCTGATCAACCGGTTCAGGCGTGAGGTCATCAGGGCCGGCGTGTTCCGTGAGTGCAAGAAGAGGAGGTTTTTCGAGAACAGCCAGGACAAGAAGAAGAGAGCTGGCTTCATTTCCAAGAAAAAGAAGAAGAGAAAGGCTAGGCAGGCAGCTAGAAGGAACAAGAATAAGAACAATTACCCCCAAAGGTCTTCGCAATCAAGATATCGGAACCAGTTCAAACAGCAAAAACCCGAGAAGGACAACGAACCCAAGAGGAAAGAGGACAGTGACAATGATGATAACTGGGACTTCTATGAAGTAGACCTTCCTTATAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ribosomal_S21

Protein Analysis

202

Amino Acids

23.0

Weight (kDa)

10.18

Isoelectric Point (pI)

74.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S21 PF01165 85 - 129 2.5e-12 Ribosomal protein S21
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 600
Acc36I ACCTGC 1 cut(s) 228
AccB1I GGYRCC 1 cut(s) 27
AccB7I CCANNNNNTGG 1 cut(s) 175
AccBSI CCGCTC 1 cut(s) 159
AccI GTMKAC 1 cut(s) 588
AciI CCGC 6 cut(s) 46, 87, 96, 99, 117, 157
AcoI YGGCCR 4 cut(s) 43, 166, 207, 237
AcuI CTGAAG 1 cut(s) 214
AcvI CACGTG 1 cut(s) 123
AfiI CCNNNNNNNGG 2 cut(s) 175, 259
AflIII ACRYGT 1 cut(s) 122
AgeI ACCGGT 1 cut(s) 297
AgsI TTSAA 2 cut(s) 247, 505
AjnI CCWGG 3 cut(s) 252, 264, 369
AluBI AGCT 5 cut(s) 62, 172, 234, 389, 437
AluI AGCT 5 cut(s) 62, 172, 234, 389, 437
AlwNI CAGNNNCTG 1 cut(s) 175
Ama87I CYCGRG 2 cut(s) 19, 518
AoxI GGCC 8 cut(s) 16, 43, 148, 166, 178, 207, 237, 321
ApaI GGGCCC 3 cut(s) 20, 152, 182
ApeKI GCWGC 2 cut(s) 172, 434
AsiGI ACCGGT 1 cut(s) 297
Asp700I GAANNNNTTC 1 cut(s) 500
AspS9I GGNCC 8 cut(s) 16, 17, 148, 149, 178, 179, 262, 321
AsuC2I CCSGG 3 cut(s) 20, 21, 241
AsuHPI GGTGA 1 cut(s) 268
AvaI CYCGRG 2 cut(s) 19, 518
AvaII GGWCC 1 cut(s) 262
BaeGI GKGCMC 3 cut(s) 20, 152, 182
BalI TGGCCA 2 cut(s) 168, 209
BanI GGYRCC 1 cut(s) 27
BanII GRGCYC 3 cut(s) 20, 152, 182
BbrPI CACGTG 1 cut(s) 123
BbsI GAAGAC 1 cut(s) 468
BbvI GCAGC 2 cut(s) 159, 446
BccI CCATC 1 cut(s) 191
BciT130I CCWGG 3 cut(s) 254, 266, 371
BclI TGATCA 1 cut(s) 291
BcnI CCSGG 3 cut(s) 20, 21, 241
BfaI CTAG 2 cut(s) 426, 438
BfuAI ACCTGC 1 cut(s) 228
BisI GCNGC 4 cut(s) 46, 99, 173, 435
BlsI GCNGC 4 cut(s) 47, 100, 174, 436
Bme1390I CCNGG 6 cut(s) 20, 21, 241, 254, 266, 371
Bme18I GGWCC 1 cut(s) 262
BmeT110I CYCGRG 2 cut(s) 19, 518
BmgT120I GGNCC 8 cut(s) 16, 17, 148, 149, 178, 179, 262, 321
BmiI GGNNCC 7 cut(s) 18, 29, 150, 151, 180, 181, 497
BmrFI CCNGG 6 cut(s) 20, 21, 241, 254, 266, 371
BmrI ACTGGG 1 cut(s) 580
BmuI ACTGGG 1 cut(s) 580
BpiI GAAGAC 1 cut(s) 468
BpuMI CCSGG 3 cut(s) 20, 21, 241
BsaAI YACGTR 1 cut(s) 123
BsaJI CCNNGG 3 cut(s) 19, 40, 252
BsaWI WCCGGW 1 cut(s) 297
Bsc4I CCNNNNNNNGG 2 cut(s) 175, 259
Bse118I RCCGGY 2 cut(s) 297, 323
Bse1I ACTGG 2 cut(s) 499, 575
BseBI CCWGG 3 cut(s) 254, 266, 371
BseDI CCNNGG 3 cut(s) 19, 40, 252
BseLI CCNNNNNNNGG 2 cut(s) 175, 259
BseNI ACTGG 2 cut(s) 499, 575
BseRI GAGGAG 8 cut(s) 54, 59, 66, 69, 72, 103, 296, 364
BseSI GKGCMC 3 cut(s) 20, 152, 182
BseX3I CGGCCG 1 cut(s) 237
BseXI GCAGC 2 cut(s) 159, 446
BseYI CCCAGC 1 cut(s) 175
Bsh1285I CGRYCG 1 cut(s) 240
BshFI GGCC 8 cut(s) 18, 45, 150, 168, 180, 209, 239, 323
BshNI GGYRCC 1 cut(s) 27
BshTI ACCGGT 1 cut(s) 297
BsiEI CGRYCG 1 cut(s) 240
BsiHKCI CYCGRG 2 cut(s) 19, 518
BsiSI CCGG 5 cut(s) 20, 240, 278, 298, 324
BslFI GGGAC 2 cut(s) 111, 587
BslI CCNNNNNNNGG 2 cut(s) 175, 259
BsmFI GGGAC 2 cut(s) 111, 587
BsnI GGCC 8 cut(s) 18, 45, 150, 168, 180, 209, 239, 323
BsoBI CYCGRG 2 cut(s) 19, 518
Bsp120I GGGCCC 3 cut(s) 16, 148, 178
Bsp1286I GDGCHC 3 cut(s) 20, 152, 182
Bsp143I GATC 1 cut(s) 291
Bsp19I CCATGG 1 cut(s) 40
BspACI CCGC 6 cut(s) 46, 87, 96, 99, 117, 157
BspANI GGCC 8 cut(s) 18, 45, 150, 168, 180, 209, 239, 323
BspLI GGNNCC 7 cut(s) 18, 29, 150, 151, 180, 181, 497
BspMI ACCTGC 1 cut(s) 228
BspQI GCTCTTC 1 cut(s) 230
BspT107I GGYRCC 1 cut(s) 27
BsrBI CCGCTC 1 cut(s) 159
BsrFI RCCGGY 2 cut(s) 297, 323
BsrI ACTGG 2 cut(s) 499, 575
BssAI RCCGGY 2 cut(s) 297, 323
BssECI CCNNGG 3 cut(s) 19, 40, 252
BssMI GATC 1 cut(s) 291
BssT1I CCWWGG 1 cut(s) 40
Bst2UI CCWGG 3 cut(s) 254, 266, 371
Bst4CI ACNGT 2 cut(s) 262, 554
Bst6I CTCTTC 5 cut(s) 142, 230, 341, 377, 410
BstBAI YACGTR 1 cut(s) 123
BstC8I GCNNGC 5 cut(s) 170, 207, 325, 391, 432
BstDSI CCRYGG 1 cut(s) 40
BstKTI GATC 1 cut(s) 294
BstMBI GATC 1 cut(s) 291
BstMCI CGRYCG 1 cut(s) 240
BstMWI GCNNNNNNNGC 5 cut(s) 45, 95, 156, 165, 431
BstNI CCWGG 3 cut(s) 254, 266, 371
BstSCI CCNGG 6 cut(s) 18, 19, 239, 252, 264, 369
BstSLI GKGCMC 3 cut(s) 20, 152, 182
BstV1I GCAGC 2 cut(s) 159, 446
BstV2I GAAGAC 1 cut(s) 468
BstZI CGGCCG 1 cut(s) 237
BsuRI GGCC 8 cut(s) 18, 45, 150, 168, 180, 209, 239, 323
BtgI CCRYGG 1 cut(s) 40
BtsIMutI CAGTG 1 cut(s) 559
BveI ACCTGC 1 cut(s) 228
Cac8I GCNNGC 5 cut(s) 170, 207, 325, 391, 432
CaiI CAGNNNCTG 1 cut(s) 175
Cfr10I RCCGGY 2 cut(s) 297, 323
Cfr13I GGNCC 8 cut(s) 16, 17, 148, 149, 178, 179, 262, 321
Cfr9I CCCGGG 1 cut(s) 19
CspAI ACCGGT 1 cut(s) 297
CviAII CATG 1 cut(s) 41
DpnI GATC 1 cut(s) 293
DpnII GATC 1 cut(s) 291
EaeI YGGCCR 4 cut(s) 43, 166, 207, 237
EagI CGGCCG 1 cut(s) 237
Eam1104I CTCTTC 5 cut(s) 142, 230, 341, 377, 410
EarI CTCTTC 5 cut(s) 142, 230, 341, 377, 410
EciI GGCGGA 1 cut(s) 106
EclXI CGGCCG 1 cut(s) 237
Eco130I CCWWGG 1 cut(s) 40
Eco24I GRGCYC 3 cut(s) 20, 152, 182
Eco32I GATATC 1 cut(s) 491
Eco47I GGWCC 1 cut(s) 262
Eco52I CGGCCG 1 cut(s) 237
Eco57I CTGAAG 1 cut(s) 214
Eco72I CACGTG 1 cut(s) 123
Eco88I CYCGRG 2 cut(s) 19, 518
EcoO109I RGGNCCY 3 cut(s) 148, 149, 179
EcoRII CCWGG 3 cut(s) 252, 264, 369
EcoRV GATATC 1 cut(s) 491
EcoT14I CCWWGG 1 cut(s) 40
EcoT38I GRGCYC 3 cut(s) 20, 152, 182
ErhI CCWWGG 1 cut(s) 40
FaeI CATG 1 cut(s) 44
FaiI YATR 3 cut(s) 42, 582, 600
FalI AAGNNNNNCTT 1 cut(s) 576
FaqI GGGAC 2 cut(s) 111, 587
FatI CATG 1 cut(s) 40
FauI CCCGC 2 cut(s) 94, 164
FbaI TGATCA 1 cut(s) 291
FblI GTMKAC 1 cut(s) 588
Fnu4HI GCNGC 4 cut(s) 46, 99, 173, 435
FriOI GRGCYC 3 cut(s) 20, 152, 182
Fsp4HI GCNGC 4 cut(s) 46, 99, 173, 435
FspBI CTAG 2 cut(s) 426, 438
GluI GCNGC 4 cut(s) 46, 99, 173, 435
GsaI CCCAGC 1 cut(s) 179
HaeIII GGCC 8 cut(s) 18, 45, 150, 168, 180, 209, 239, 323
HapII CCGG 5 cut(s) 20, 240, 278, 298, 324
Hin1II CATG 1 cut(s) 44
HpaII CCGG 5 cut(s) 20, 240, 278, 298, 324
HphI GGTGA 1 cut(s) 268
Hpy166II GTNNAC 1 cut(s) 589
Hpy188I TCNGA 1 cut(s) 495
Hpy188III TCNNGA 2 cut(s) 361, 486
Hpy8I GTNNAC 1 cut(s) 589
HpyAV CCTTC 4 cut(s) 206, 435, 517, 602
HpyCH4III ACNGT 2 cut(s) 262, 554
HpyCH4IV ACGT 1 cut(s) 122
HpyCH4V TGCA 1 cut(s) 342
HpyF10VI GCNNNNNNNGC 5 cut(s) 45, 95, 156, 165, 431
HpySE526I ACGT 1 cut(s) 122
Hsp92II CATG 1 cut(s) 44
KroI GCCGGC 1 cut(s) 323
KroNI GCCGGC 1 cut(s) 325
Ksp22I TGATCA 1 cut(s) 291
Kzo9I GATC 1 cut(s) 291
LguI GCTCTTC 1 cut(s) 230
LmnI GCTCC 1 cut(s) 67
Lsp1109I GCAGC 2 cut(s) 159, 446
MaeI CTAG 2 cut(s) 426, 438
MaeII ACGT 1 cut(s) 122
MaeIII GTNAC 2 cut(s) 256, 554
MalI GATC 1 cut(s) 293
MbiI CCGCTC 1 cut(s) 159
MboI GATC 1 cut(s) 291
MboII GAAGA 9 cut(s) 129, 217, 220, 358, 391, 394, 424, 427, 468
MhlI GDGCHC 3 cut(s) 20, 152, 182
MlsI TGGCCA 2 cut(s) 168, 209
MluCI AATT 2 cut(s) 460, 601
MluNI TGGCCA 2 cut(s) 168, 209
Mox20I TGGCCA 2 cut(s) 168, 209
MroNI GCCGGC 1 cut(s) 323
MroXI GAANNNNTTC 1 cut(s) 500
MscI TGGCCA 2 cut(s) 168, 209
Msp20I TGGCCA 2 cut(s) 168, 209
MspA1I CMGCKG 1 cut(s) 172
MspI CCGG 5 cut(s) 20, 240, 278, 298, 324
MspR9I CCNGG 6 cut(s) 20, 21, 241, 254, 266, 371
MvaI CCWGG 3 cut(s) 254, 266, 371
MwoI GCNNNNNNNGC 5 cut(s) 45, 95, 156, 165, 431
NaeI GCCGGC 1 cut(s) 325
NciI CCSGG 3 cut(s) 20, 21, 241
NcoI CCATGG 1 cut(s) 40
NdeII GATC 1 cut(s) 291
NgoMIV GCCGGC 1 cut(s) 323
NlaIII CATG 1 cut(s) 44
NlaIV GGNNCC 7 cut(s) 18, 29, 150, 151, 180, 181, 497
NmeAIII GCCGAG 1 cut(s) 215
NmuCI GTSAC 2 cut(s) 256, 554
PciSI GCTCTTC 1 cut(s) 230
PdiI GCCGGC 1 cut(s) 325
PdmI GAANNNNTTC 1 cut(s) 500
PflMI CCANNNNNTGG 1 cut(s) 175
PfoI TCCNGGA 1 cut(s) 264
PinAI ACCGGT 1 cut(s) 297
PkrI GCNGC 4 cut(s) 47, 100, 174, 436
PmaCI CACGTG 1 cut(s) 123
PmlI CACGTG 1 cut(s) 123
Ppu21I YACGTR 1 cut(s) 123
PsiI TTATAA 1 cut(s) 600
Psp6I CCWGG 3 cut(s) 252, 264, 369
PspCI CACGTG 1 cut(s) 123
PspFI CCCAGC 1 cut(s) 175
PspGI CCWGG 3 cut(s) 252, 264, 369
PspN4I GGNNCC 7 cut(s) 18, 29, 150, 151, 180, 181, 497
PspOMI GGGCCC 3 cut(s) 16, 148, 178
PspPI GGNCC 8 cut(s) 16, 17, 148, 149, 178, 179, 262, 321
PstNI CAGNNNCTG 1 cut(s) 175
PvuII CAGCTG 1 cut(s) 172
SapI GCTCTTC 1 cut(s) 230
SatI GCNGC 4 cut(s) 46, 99, 173, 435
Sau3AI GATC 1 cut(s) 291
Sau96I GGNCC 8 cut(s) 16, 17, 148, 149, 178, 179, 262, 321
ScrFI CCNGG 6 cut(s) 20, 21, 241, 254, 266, 371
SduI GDGCHC 3 cut(s) 20, 152, 182
SinI GGWCC 1 cut(s) 262
SmaI CCCGGG 1 cut(s) 21
Sse9I AATT 2 cut(s) 460, 601
SsiI CCGC 6 cut(s) 46, 87, 96, 99, 117, 157
SspMI CTAG 2 cut(s) 426, 438
StyD4I CCNGG 6 cut(s) 18, 19, 239, 252, 264, 369
StyI CCWWGG 1 cut(s) 40
TaaI ACNGT 2 cut(s) 262, 554
TaiI ACGT 1 cut(s) 125
TaqI TCGA 1 cut(s) 360
TasI AATT 2 cut(s) 460, 601
TauI GCSGC 2 cut(s) 48, 101
TscAI CASTG 1 cut(s) 559
TseFI GTSAC 2 cut(s) 256, 554
TseI GCWGC 2 cut(s) 172, 434
Tsp45I GTSAC 2 cut(s) 256, 554
TspDTI ATGAA 2 cut(s) 385, 597
TspGWI ACGGA 1 cut(s) 323
TspMI CCCGGG 1 cut(s) 19
TspRI CASTG 1 cut(s) 559
Van91I CCANNNNNTGG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 262
XcmI CCANNNNNNNNNTGG 1 cut(s) 38
XmaI CCCGGG 1 cut(s) 19
XmiI GTMKAC 1 cut(s) 588
XmnI GAANNNNTTC 1 cut(s) 500
XspI CTAG 2 cut(s) 426, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.