AT3G62580

Domain of unknown function (DUF4149)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0003018
Physical Location & Seq
Forward (+)
23146809 .. 23147951
1143 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G62580.1

Sequence Viewer

Length: 642 bp
ATGGGTTGGTATACCAGTTTCTTAGTAGTGGTGGCGTCCTTAGCCGTCGGAGTTATATTCTCGCCGGAGACATTCGGATCGTTGACGAATGGAGAGAATTCGGCGAAGATTTCGATATTCCTGAAGCTAGCTCATCTACTCAGTTTCGCTACAGCTTGGGGAGCTGCTCTCTGGGCTACCTTCATCGGAGGAATCATCATGTTCAAGAATTTACCGAGGCATCAGTTTGGTAATCTTCAGAGCAAGTTGTTTCCAGCTTACTTCACTTTGGTTGGTTCTTGCTGCGCAATTTCACTCTCTGCTTTTGGTTATTTGCATCCATGGAAGTCATCTTCTACGGTCGAGAAATATCAGATAGGGTTTCTCCTATCCGCTTTTGCTTTCAATCTCACCAATTTGTTTGTCTTCACTCCCATGACCATCGATATGATGAAACAAAGGCACAAAGTAGAGAGGGAAAACAACATTGGAGATGAAGTTGGTTGGTCCAAGAACAGAGAAAAGGCAAAGAGTATCCCAAAGCTAGCTGCTATGAACAAGAAGTTTGGAATGATTCACGGGTTATCATCCCTTGCTAACATCTTTTCATTTGGAAGCCTTGCTATGCATTCTTGGTATCTAGCTGGGAAGTTGAATCTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

213

Amino Acids

23.58

Weight (kDa)

9.76

Isoelectric Point (pI)

26.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 47 - 149 1.5e-30 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 286
AccI GTMKAC 1 cut(s) 11
AciI CCGC 1 cut(s) 372
AclWI GGATC 1 cut(s) 85
AcsI RAATTY 2 cut(s) 97, 208
AcuI CTGAAG 2 cut(s) 143, 221
AcyI GRCGYC 1 cut(s) 35
AgsI TTSAA 3 cut(s) 205, 385, 634
AjuI GAANNNNNNNTTGG 2 cut(s) 450, 482
AluBI AGCT 8 cut(s) 127, 131, 155, 164, 257, 523, 527, 623
AluI AGCT 8 cut(s) 127, 131, 155, 164, 257, 523, 527, 623
Alw26I GTCTC 1 cut(s) 62
AlwI GGATC 1 cut(s) 85
ApeKI GCWGC 3 cut(s) 164, 282, 527
ApoI RAATTY 2 cut(s) 97, 208
AspLEI GCGC 1 cut(s) 287
AspS9I GGNCC 1 cut(s) 486
AsuHPI GGTGA 1 cut(s) 382
AsuNHI GCTAGC 2 cut(s) 127, 523
AvaII GGWCC 1 cut(s) 486
BbsI GAAGAC 1 cut(s) 397
BbvI GCAGC 3 cut(s) 151, 269, 514
BccI CCATC 1 cut(s) 428
BceAI ACGGC 1 cut(s) 29
BciVI GTATCC 1 cut(s) 524
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 4 cut(s) 128, 524, 620, 640
BfmI CTRYAG 1 cut(s) 150
BfuI GTATCC 1 cut(s) 524
BisI GCNGC 3 cut(s) 165, 283, 528
BlsI GCNGC 3 cut(s) 166, 284, 529
Bme18I GGWCC 1 cut(s) 486
BmgT120I GGNCC 1 cut(s) 486
BmsI GCATC 2 cut(s) 229, 325
BmtI GCTAGC 2 cut(s) 131, 527
BpiI GAAGAC 1 cut(s) 397
BplI GAGNNNNNCTC 2 cut(s) 153, 185
Bpu10I CCTNAGC 1 cut(s) 40
Bsa29I ATCGAT 1 cut(s) 423
BsaHI GRCGYC 1 cut(s) 35
BsaJI CCNNGG 2 cut(s) 215, 320
BsaXI ACNNNNNCTCC 2 cut(s) 462, 492
Bse1I ACTGG 1 cut(s) 15
BseCI ATCGAT 1 cut(s) 423
BseDI CCNNGG 2 cut(s) 215, 320
BseGI GGATG 2 cut(s) 316, 566
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 1 cut(s) 15
BseXI GCAGC 3 cut(s) 151, 269, 514
BseYI CCCAGC 1 cut(s) 623
Bsh1285I CGRYCG 1 cut(s) 342
BshVI ATCGAT 1 cut(s) 423
BsiEI CGRYCG 1 cut(s) 342
BsiSI CCGG 1 cut(s) 65
BsmAI GTCTC 1 cut(s) 62
BsmI GAATGC 1 cut(s) 607
Bsp143I GATC 1 cut(s) 77
Bsp19I CCATGG 1 cut(s) 320
BspACI CCGC 1 cut(s) 372
BspCNI CTCAG 1 cut(s) 153
BspDI ATCGAT 1 cut(s) 423
BspOI GCTAGC 2 cut(s) 131, 527
BspPI GGATC 1 cut(s) 85
BsrI ACTGG 1 cut(s) 15
BssECI CCNNGG 2 cut(s) 215, 320
BssMI GATC 1 cut(s) 77
BssNAI GTATAC 1 cut(s) 12
BssNI GRCGYC 1 cut(s) 35
BssT1I CCWWGG 1 cut(s) 320
Bst1107I GTATAC 1 cut(s) 12
Bst4CI ACNGT 1 cut(s) 340
BstACI GRCGYC 1 cut(s) 35
BstC8I GCNNGC 2 cut(s) 129, 525
BstDEI CTNAG 3 cut(s) 22, 40, 140
BstDSI CCRYGG 1 cut(s) 320
BstF5I GGATG 2 cut(s) 316, 566
BstHHI GCGC 1 cut(s) 287
BstKTI GATC 1 cut(s) 80
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 1 cut(s) 77
BstMCI CGRYCG 1 cut(s) 342
BstMWI GCNNNNNNNGC 3 cut(s) 41, 161, 173
BstSFI CTRYAG 1 cut(s) 150
BstV1I GCAGC 3 cut(s) 151, 269, 514
BstV2I GAAGAC 1 cut(s) 397
BstZ17I GTATAC 1 cut(s) 12
Bsu15I ATCGAT 1 cut(s) 423
BsuI GTATCC 1 cut(s) 524
BsuTUI ATCGAT 1 cut(s) 423
BtgI CCRYGG 1 cut(s) 320
BtsCI GGATG 2 cut(s) 316, 566
Cac8I GCNNGC 2 cut(s) 129, 525
CfoI GCGC 1 cut(s) 287
Cfr13I GGNCC 1 cut(s) 486
ClaI ATCGAT 1 cut(s) 423
CseI GACGC 1 cut(s) 24
CviAII CATG 3 cut(s) 199, 321, 415
DdeI CTNAG 3 cut(s) 22, 40, 140
DpnI GATC 1 cut(s) 79
DpnII GATC 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 320
Eco47I GGWCC 1 cut(s) 486
Eco57I CTGAAG 2 cut(s) 143, 221
EcoRI GAATTC 1 cut(s) 97
EcoT14I CCWWGG 1 cut(s) 320
EcoT22I ATGCAT 1 cut(s) 609
ErhI CCWWGG 1 cut(s) 320
FaeI CATG 3 cut(s) 202, 324, 418
FaiI YATR 8 cut(s) 12, 56, 200, 322, 416, 428, 533, 605
FatI CATG 3 cut(s) 198, 320, 414
FblI GTMKAC 1 cut(s) 11
Fnu4HI GCNGC 3 cut(s) 165, 283, 528
FokI GGATG 2 cut(s) 303, 553
Fsp4HI GCNGC 3 cut(s) 165, 283, 528
FspBI CTAG 4 cut(s) 128, 524, 620, 640
FspI TGCGCA 1 cut(s) 286
GlaI GCGC 1 cut(s) 286
GluI GCNGC 3 cut(s) 165, 283, 528
GsaI CCCAGC 1 cut(s) 627
HapII CCGG 1 cut(s) 65
HgaI GACGC 1 cut(s) 24
HhaI GCGC 1 cut(s) 287
Hin1I GRCGYC 1 cut(s) 35
Hin1II CATG 3 cut(s) 202, 324, 418
Hin6I GCGC 1 cut(s) 285
HinP1I GCGC 1 cut(s) 285
HincII GTYRAC 1 cut(s) 84
HindII GTYRAC 1 cut(s) 84
HinfI GANTC 3 cut(s) 192, 553, 634
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 382
Hpy166II GTNNAC 2 cut(s) 12, 84
Hpy188I TCNGA 5 cut(s) 50, 77, 188, 240, 354
Hpy188III TCNNGA 3 cut(s) 121, 205, 343
Hpy8I GTNNAC 2 cut(s) 12, 84
Hpy99I CGWCG 1 cut(s) 50
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 340
HpyCH4V TGCA 2 cut(s) 316, 607
HpyF10VI GCNNNNNNNGC 3 cut(s) 41, 161, 173
HpyF3I CTNAG 3 cut(s) 22, 40, 140
Hsp92I GRCGYC 1 cut(s) 35
Hsp92II CATG 3 cut(s) 202, 324, 418
HspAI GCGC 1 cut(s) 285
Kzo9I GATC 1 cut(s) 77
LmnI GCTCC 1 cut(s) 161
LpnPI CCDG 6 cut(s) 28, 78, 134, 157, 267, 609
Lsp1109I GCAGC 3 cut(s) 151, 269, 514
LweI GCATC 2 cut(s) 229, 325
MaeI CTAG 4 cut(s) 128, 524, 620, 640
MalI GATC 1 cut(s) 79
MboI GATC 1 cut(s) 77
MboII GAAGA 4 cut(s) 118, 227, 324, 397
MluCI AATT 4 cut(s) 97, 208, 288, 394
MmeI TCCRAC 1 cut(s) 28
MnlI CCTC 3 cut(s) 182, 210, 447
Mph1103I ATGCAT 1 cut(s) 609
MslI CAYNNNNRTG 2 cut(s) 413, 425
MspI CCGG 1 cut(s) 65
Mva1269I GAATGC 1 cut(s) 607
MwoI GCNNNNNNNGC 3 cut(s) 41, 161, 173
NcoI CCATGG 1 cut(s) 320
NdeII GATC 1 cut(s) 77
NheI GCTAGC 2 cut(s) 127, 523
NlaIII CATG 3 cut(s) 202, 324, 418
NsbI TGCGCA 1 cut(s) 286
NsiI ATGCAT 1 cut(s) 609
PctI GAATGC 1 cut(s) 607
PfeI GAWTC 3 cut(s) 192, 553, 634
PkrI GCNGC 3 cut(s) 166, 284, 529
PspFI CCCAGC 1 cut(s) 623
PspPI GGNCC 1 cut(s) 486
RseI CAYNNNNRTG 2 cut(s) 413, 425
SatI GCNGC 3 cut(s) 165, 283, 528
Sau3AI GATC 1 cut(s) 77
Sau96I GGNCC 1 cut(s) 486
SetI ASST 9 cut(s) 129, 133, 157, 166, 182, 259, 525, 529, 625
SfaNI GCATC 2 cut(s) 229, 325
SfcI CTRYAG 1 cut(s) 150
SinI GGWCC 1 cut(s) 486
SmiMI CAYNNNNRTG 2 cut(s) 413, 425
Sse9I AATT 4 cut(s) 97, 208, 288, 394
SsiI CCGC 1 cut(s) 372
SspMI CTAG 4 cut(s) 128, 524, 620, 640
StyI CCWWGG 1 cut(s) 320
TaaI ACNGT 1 cut(s) 340
TaqI TCGA 3 cut(s) 113, 342, 423
TasI AATT 4 cut(s) 97, 208, 288, 394
TfiI GAWTC 3 cut(s) 192, 553, 634
TseI GCWGC 3 cut(s) 164, 282, 527
TspDTI ATGAA 5 cut(s) 172, 446, 489, 548, 576
VpaK11BI GGWCC 1 cut(s) 486
XapI RAATTY 2 cut(s) 97, 208
XmiI GTMKAC 1 cut(s) 11
XspI CTAG 4 cut(s) 128, 524, 620, 640
Zsp2I ATGCAT 1 cut(s) 609
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.