MD04G1089900.v1.1

Transmembrane protein 205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003018
Physical Location & Seq
Reverse (-)
15963903 .. 15965895
1993 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1089900.v1.1.491

Sequence Viewer

Length: 600 bp
ATGACTCGCTTCGTATGTGGTGAATGGATAAAGTCACACAACTTACATTGTGCAGGTCTAATGAGGAGTGGTAGGAGTTGCAGGATACAGTGGATGAACTACGTCCGCCTAAACATTAAGCATGGTAAGATTACCATCATAGAAGAGAAAACCATTCTCCAACTTCATCAACGTTGGGGTAACAACCGTGGCTTTCTTGGCCATCAGAGTGATATTCTCCCCGGAGACCTTTGGGTGGAAATCGGATGGTCTAAAATCGCCCACCCTCTCCACTACCTAAAGTTGGCACATCTGCTCTGTTTCTCCACCACATTCGGCGCCGCTCTTTGGGTCACCTTCATAGGCGGCATCATCATGTTCAAGAATCTTTCACGCAATCAGTTCGGGAATCTTCAGAGCAAGATGTTTCCGGTGTACTTCACGATGGTGGGGATATGCCTTGCAGTATCGGCTGGGTCGTTTGGGTATTTGCATCCATGGAGCTCTTCCTCTGTTGCCGACAAGTACCAGCTCGACTTTTTGCTCTCTGCCTTTGCTTTCAATCTCACCAATCTCTTCGTCTTCACTCCCATGACCATCAAGGTAATTGCTAGTTACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

200

Amino Acids

22.71

Weight (kDa)

9.64

Isoelectric Point (pI)

38.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 99 - 195 6.7e-25 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 44
AccB1I GGYRCC 1 cut(s) 317
AccBSI CCGCTC 1 cut(s) 323
AciI CCGC 3 cut(s) 106, 321, 345
AclI AACGTT 1 cut(s) 172
AcoI YGGCCR 1 cut(s) 199
AcuI CTGAAG 1 cut(s) 377
AcyI GRCGYC 1 cut(s) 318
AfaI GTAC 2 cut(s) 416, 506
AfiI CCNNNNNNNGG 3 cut(s) 235, 283, 327
AgsI TTSAA 2 cut(s) 361, 541
AleI CACNNNNGTG 1 cut(s) 425
AluBI AGCT 2 cut(s) 483, 511
AluI AGCT 2 cut(s) 483, 511
Alw21I GWGCWC 1 cut(s) 485
Alw26I GTCTC 1 cut(s) 219
AoxI GGCC 1 cut(s) 199
AspLEI GCGC 1 cut(s) 320
AsuC2I CCSGG 1 cut(s) 222
AsuHPI GGTGA 3 cut(s) 32, 325, 538
BalI TGGCCA 1 cut(s) 201
BanI GGYRCC 1 cut(s) 317
BanII GRGCYC 1 cut(s) 485
BbsI GAAGAC 1 cut(s) 553
Bbv12I GWGCWC 1 cut(s) 485
BccI CCATC 5 cut(s) 143, 210, 240, 418, 584
BcgI CGANNNNNNTGC 2 cut(s) 364, 398
BciVI GTATCC 1 cut(s) 78
BcnI CCSGG 1 cut(s) 222
BcoDI GTCTC 1 cut(s) 219
BfaI CTAG 2 cut(s) 591, 598
BfoI RGCGCY 1 cut(s) 321
BfuAI ACCTGC 1 cut(s) 44
BfuI GTATCC 1 cut(s) 78
BisI GCNGC 2 cut(s) 321, 346
BlsI GCNGC 2 cut(s) 322, 347
Bme1390I CCNGG 1 cut(s) 222
BmiI GGNNCC 1 cut(s) 319
BmrFI CCNGG 1 cut(s) 222
BmsI GCATC 2 cut(s) 357, 481
BpiI GAAGAC 1 cut(s) 553
BpuMI CCSGG 1 cut(s) 222
BsaBI GATNNNNATC 1 cut(s) 134
BsaHI GRCGYC 1 cut(s) 318
BsaI GGTCTC 1 cut(s) 219
BsaJI CCNNGG 3 cut(s) 187, 220, 476
BsaWI WCCGGW 1 cut(s) 409
Bsc4I CCNNNNNNNGG 3 cut(s) 235, 283, 327
Bse8I GATNNNNATC 1 cut(s) 134
BseDI CCNNGG 3 cut(s) 187, 220, 476
BseGI GGATG 3 cut(s) 99, 251, 472
BseJI GATNNNNATC 1 cut(s) 134
BseLI CCNNNNNNNGG 3 cut(s) 235, 283, 327
BseRI GAGGAG 1 cut(s) 79
BseYI CCCAGC 1 cut(s) 452
BsgI GTGCAG 1 cut(s) 72
BshFI GGCC 1 cut(s) 201
BshNI GGYRCC 1 cut(s) 317
BsiHKAI GWGCWC 1 cut(s) 485
BsiSI CCGG 2 cut(s) 222, 410
BslI CCNNNNNNNGG 3 cut(s) 235, 283, 327
BsmAI GTCTC 1 cut(s) 219
BsnI GGCC 1 cut(s) 201
Bso31I GGTCTC 1 cut(s) 219
Bsp1286I GDGCHC 1 cut(s) 485
Bsp19I CCATGG 1 cut(s) 476
BspACI CCGC 3 cut(s) 106, 321, 345
BspANI GGCC 1 cut(s) 201
BspLI GGNNCC 1 cut(s) 319
BspMI ACCTGC 1 cut(s) 44
BspQI GCTCTTC 1 cut(s) 490
BspT107I GGYRCC 1 cut(s) 317
BspTNI GGTCTC 1 cut(s) 219
BsrBI CCGCTC 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 187, 220, 476
BssNI GRCGYC 1 cut(s) 318
BssT1I CCWWGG 1 cut(s) 476
Bst4CI ACNGT 2 cut(s) 90, 188
Bst6I CTCTTC 3 cut(s) 138, 490, 560
BstACI GRCGYC 1 cut(s) 318
BstDSI CCRYGG 2 cut(s) 187, 476
BstEII GGTNACC 1 cut(s) 331
BstF5I GGATG 3 cut(s) 99, 251, 472
BstH2I RGCGCY 1 cut(s) 321
BstHHI GCGC 1 cut(s) 320
BstMAI GTCTC 1 cut(s) 219
BstMWI GCNNNNNNNGC 2 cut(s) 198, 449
BstPI GGTNACC 1 cut(s) 331
BstSCI CCNGG 1 cut(s) 220
BstV2I GAAGAC 1 cut(s) 553
BsuI GTATCC 1 cut(s) 78
BsuRI GGCC 1 cut(s) 201
BtgI CCRYGG 2 cut(s) 187, 476
BtsCI GGATG 3 cut(s) 99, 251, 472
BtsIMutI CAGTG 1 cut(s) 95
BveI ACCTGC 1 cut(s) 44
CfoI GCGC 1 cut(s) 320
Csp6I GTAC 2 cut(s) 415, 505
CviAII CATG 4 cut(s) 122, 355, 477, 571
CviJI RGCY 5 cut(s) 192, 201, 452, 483, 511
CviKI_1 RGCY 5 cut(s) 192, 201, 452, 483, 511
CviQI GTAC 2 cut(s) 415, 505
DinI GGCGCC 1 cut(s) 319
EaeI YGGCCR 1 cut(s) 199
Eam1104I CTCTTC 3 cut(s) 138, 490, 560
EarI CTCTTC 3 cut(s) 138, 490, 560
EciI GGCGGA 1 cut(s) 95
Ecl136II GAGCTC 1 cut(s) 483
Eco130I CCWWGG 1 cut(s) 476
Eco24I GRGCYC 1 cut(s) 485
Eco31I GGTCTC 1 cut(s) 219
Eco53kI GAGCTC 1 cut(s) 483
Eco57I CTGAAG 1 cut(s) 377
Eco91I GGTNACC 1 cut(s) 331
EcoICRI GAGCTC 1 cut(s) 483
EcoO65I GGTNACC 1 cut(s) 331
EcoT14I CCWWGG 1 cut(s) 476
EcoT38I GRGCYC 1 cut(s) 485
EgeI GGCGCC 1 cut(s) 319
EheI GGCGCC 1 cut(s) 319
ErhI CCWWGG 1 cut(s) 476
FaeI CATG 4 cut(s) 125, 358, 480, 574
FaiI YATR 8 cut(s) 16, 123, 140, 341, 356, 436, 478, 572
FatI CATG 4 cut(s) 121, 354, 476, 570
Fnu4HI GCNGC 2 cut(s) 321, 346
FokI GGATG 3 cut(s) 106, 258, 459
FriOI GRGCYC 1 cut(s) 485
Fsp4HI GCNGC 2 cut(s) 321, 346
FspBI CTAG 2 cut(s) 591, 598
GlaI GCGC 1 cut(s) 319
GluI GCNGC 2 cut(s) 321, 346
GsaI CCCAGC 1 cut(s) 456
HaeII RGCGCY 1 cut(s) 321
HaeIII GGCC 1 cut(s) 201
HapII CCGG 2 cut(s) 222, 410
HhaI GCGC 1 cut(s) 320
Hin1I GRCGYC 1 cut(s) 318
Hin1II CATG 4 cut(s) 125, 358, 480, 574
Hin6I GCGC 1 cut(s) 318
HinP1I GCGC 1 cut(s) 318
HinfI GANTC 3 cut(s) 4, 364, 388
HpaII CCGG 2 cut(s) 222, 410
HphI GGTGA 3 cut(s) 32, 325, 538
Hpy166II GTNNAC 1 cut(s) 415
Hpy188I TCNGA 3 cut(s) 207, 245, 396
Hpy188III TCNNGA 3 cut(s) 361, 385, 421
Hpy8I GTNNAC 1 cut(s) 415
HpyAV CCTTC 1 cut(s) 346
HpyCH4III ACNGT 2 cut(s) 90, 188
HpyCH4IV ACGT 2 cut(s) 102, 172
HpyCH4V TGCA 4 cut(s) 53, 81, 443, 472
HpyF10VI GCNNNNNNNGC 2 cut(s) 198, 449
HpySE526I ACGT 2 cut(s) 102, 172
Hsp92I GRCGYC 1 cut(s) 318
Hsp92II CATG 4 cut(s) 125, 358, 480, 574
HspAI GCGC 1 cut(s) 318
KasI GGCGCC 1 cut(s) 317
LguI GCTCTTC 1 cut(s) 490
LmnI GCTCC 1 cut(s) 480
LpnPI CCDG 6 cut(s) 39, 67, 235, 423, 438, 521
LweI GCATC 2 cut(s) 357, 481
MaeI CTAG 2 cut(s) 591, 598
MaeII ACGT 2 cut(s) 102, 172
MaeIII GTNAC 4 cut(s) 33, 179, 331, 593
MbiI CCGCTC 1 cut(s) 323
MboII GAAGA 5 cut(s) 155, 383, 477, 547, 553
MhlI GDGCHC 1 cut(s) 485
MlsI TGGCCA 1 cut(s) 201
MluCI AATT 1 cut(s) 585
MluNI TGGCCA 1 cut(s) 201
Mly113I GGCGCC 1 cut(s) 318
MmeI TCCRAC 1 cut(s) 184
MnlI CCTC 3 cut(s) 57, 276, 499
Mox20I TGGCCA 1 cut(s) 201
MscI TGGCCA 1 cut(s) 201
MseI TTAA 1 cut(s) 117
MslI CAYNNNNRTG 4 cut(s) 207, 353, 425, 569
Msp20I TGGCCA 1 cut(s) 201
MspI CCGG 2 cut(s) 222, 410
MspR9I CCNGG 1 cut(s) 222
MwoI GCNNNNNNNGC 2 cut(s) 198, 449
NarI GGCGCC 1 cut(s) 318
NciI CCSGG 1 cut(s) 222
NcoI CCATGG 1 cut(s) 476
NlaIII CATG 4 cut(s) 125, 358, 480, 574
NlaIV GGNNCC 1 cut(s) 319
NmuCI GTSAC 2 cut(s) 33, 331
OliI CACNNNNGTG 1 cut(s) 425
PciSI GCTCTTC 1 cut(s) 490
PcsI WCGNNNNNNNCGW 1 cut(s) 455
PfeI GAWTC 2 cut(s) 364, 388
PkrI GCNGC 2 cut(s) 322, 347
PluTI GGCGCC 1 cut(s) 321
Psp124BI GAGCTC 1 cut(s) 485
Psp1406I AACGTT 1 cut(s) 172
PspEI GGTNACC 1 cut(s) 331
PspFI CCCAGC 1 cut(s) 452
PspN4I GGNNCC 1 cut(s) 319
RsaI GTAC 2 cut(s) 416, 506
RsaNI GTAC 2 cut(s) 415, 505
RseI CAYNNNNRTG 4 cut(s) 207, 353, 425, 569
SacI GAGCTC 1 cut(s) 485
SapI GCTCTTC 1 cut(s) 490
SaqAI TTAA 1 cut(s) 117
SatI GCNGC 2 cut(s) 321, 346
ScrFI CCNGG 1 cut(s) 222
SduI GDGCHC 1 cut(s) 485
SetI ASST 9 cut(s) 58, 105, 175, 231, 279, 338, 485, 513, 585
SfaNI GCATC 2 cut(s) 357, 481
SfoI GGCGCC 1 cut(s) 319
SmiMI CAYNNNNRTG 4 cut(s) 207, 353, 425, 569
Sse9I AATT 1 cut(s) 585
SsiI CCGC 3 cut(s) 106, 321, 345
SspDI GGCGCC 1 cut(s) 317
SspMI CTAG 2 cut(s) 591, 598
SstI GAGCTC 1 cut(s) 485
StyD4I CCNGG 1 cut(s) 220
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 2 cut(s) 90, 188
TaiI ACGT 2 cut(s) 105, 175
TaqI TCGA 1 cut(s) 513
TasI AATT 1 cut(s) 585
TatI WGTACW 1 cut(s) 414
TauI GCSGC 2 cut(s) 323, 348
TfiI GAWTC 2 cut(s) 364, 388
Tru1I TTAA 1 cut(s) 117
Tru9I TTAA 1 cut(s) 117
TscAI CASTG 1 cut(s) 95
TseFI GTSAC 2 cut(s) 33, 331
Tsp45I GTSAC 2 cut(s) 33, 331
TspDTI ATGAA 3 cut(s) 110, 155, 328
TspRI CASTG 1 cut(s) 95
XspI CTAG 2 cut(s) 591, 598
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.