MD03G1296800.v1.1

Transmembrane protein 205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0003018
Physical Location & Seq
Forward (+)
37278889 .. 37280205
1317 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1296800.v1.1.491

Sequence Viewer

Length: 642 bp
ATGGCTTGGTTGACTCGATTCCTGGCAGCCGTGGCTTTCTTGGCCATCGGAGTGATATTCTCCCCGGAGACCTTTGGGTCGAAATCGGACGGTCTGAAATCGCCCACCCTCTCCACCTACCTAAAGCTGGCACATCTGCTCTGTTTCTCCACCGCATTCGGCGCCGCTCTCTGGGTCACCTTCATAGGCGGCATCATCATGTTCAAGAATCTTCCGCGGCATCAGTTCGGGAATCTTCAGAGCAAGATGTTTCCGGCGTACTTCACGATGGTGGGGATATGCCTTGCAGTATCAGCTGGGTCGTTTGGGTATTTGCATCCGTGGAGCTCTTCCTCTGTTGCCGACAAGTACCAGCTCGGCTTTTTGCTCTCTGCCTTTGCATTCAATCTCACCAATCTCTTCGTCTTCACTCCCATGACCATCGAGATGATGAAGCAAAGGCATAAGGTGGAGAGGGAGCAGAACATTGGGGAAGAAGTTGGATGGTCAAAGAATGTGCAAGTTGCAAAGGTAAACCCAAAACTGGCAGCCATGAATAAGAAGTTTGGTATGATTCACGGGCTATCATCTCTTGCCAACATCTTCGCATTTGGCAGCCTTGCTATGCACTCGTGGTACTTGGCTGGTAGACTTGATCTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

214

Amino Acids

23.55

Weight (kDa)

9.73

Isoelectric Point (pI)

37.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 47 - 149 3.5e-30 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 76
AccB1I GGYRCC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 167
AccI GTMKAC 1 cut(s) 628
AccII CGCG 1 cut(s) 217
AciI CCGC 5 cut(s) 153, 165, 189, 215, 217
AcoI YGGCCR 1 cut(s) 42
AcuI CTGAAG 1 cut(s) 221
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 3 cut(s) 260, 350, 617
AfiI CCNNNNNNNGG 3 cut(s) 127, 171, 523
AgsI TTSAA 2 cut(s) 205, 385
AjnI CCWGG 1 cut(s) 21
AleI CACNNNNGTG 1 cut(s) 269
AluBI AGCT 4 cut(s) 127, 296, 327, 355
AluI AGCT 4 cut(s) 127, 296, 327, 355
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 1 cut(s) 62
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 3 cut(s) 26, 527, 594
AspLEI GCGC 1 cut(s) 164
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 2 cut(s) 169, 382
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 329
BauI CACGAG 1 cut(s) 610
BbsI GAAGAC 1 cut(s) 397
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 3 cut(s) 38, 539, 606
BccI CCATC 4 cut(s) 53, 262, 428, 477
BceAI ACGGC 1 cut(s) 14
BciT130I CCWGG 1 cut(s) 23
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 62
BfoI RGCGCY 1 cut(s) 165
BisI GCNGC 6 cut(s) 27, 165, 190, 218, 528, 595
BlsI GCNGC 6 cut(s) 28, 166, 191, 219, 529, 596
Bme1390I CCNGG 2 cut(s) 23, 65
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 2 cut(s) 23, 65
BmsI GCATC 3 cut(s) 201, 229, 325
BpiI GAAGAC 1 cut(s) 397
BpuMI CCSGG 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 4 cut(s) 30, 63, 215, 320
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 171, 523
Bse1I ACTGG 1 cut(s) 528
BseBI CCWGG 1 cut(s) 23
BseDI CCNNGG 4 cut(s) 30, 63, 215, 320
BseGI GGATG 2 cut(s) 316, 488
BseLI CCNNNNNNNGG 3 cut(s) 127, 171, 523
BseNI ACTGG 1 cut(s) 528
BseXI GCAGC 3 cut(s) 38, 539, 606
BseYI CCCAGC 1 cut(s) 296
Bsh1236I CGCG 1 cut(s) 217
BshFI GGCC 1 cut(s) 44
BshNI GGYRCC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 329
BsiSI CCGG 2 cut(s) 65, 254
BslI CCNNNNNNNGG 3 cut(s) 127, 171, 523
BsmAI GTCTC 1 cut(s) 62
BsmI GAATGC 2 cut(s) 155, 380
BsnI GGCC 1 cut(s) 44
Bso31I GGTCTC 1 cut(s) 62
Bsp1286I GDGCHC 1 cut(s) 329
Bsp143I GATC 1 cut(s) 634
BspACI CCGC 5 cut(s) 153, 165, 189, 215, 217
BspANI GGCC 1 cut(s) 44
BspFNI CGCG 1 cut(s) 217
BspLI GGNNCC 1 cut(s) 163
BspQI GCTCTTC 1 cut(s) 334
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 167
BsrI ACTGG 1 cut(s) 528
BssECI CCNNGG 4 cut(s) 30, 63, 215, 320
BssMI GATC 1 cut(s) 634
BssNI GRCGYC 1 cut(s) 162
BssSI CACGAG 1 cut(s) 610
Bst2BI CACGAG 1 cut(s) 610
Bst2UI CCWGG 1 cut(s) 23
Bst4CI ACNGT 1 cut(s) 92
Bst6I CTCTTC 2 cut(s) 334, 404
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 129
BstDSI CCRYGG 3 cut(s) 30, 215, 320
BstEII GGTNACC 1 cut(s) 175
BstF5I GGATG 2 cut(s) 316, 488
BstFNI CGCG 1 cut(s) 217
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 1 cut(s) 637
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 1 cut(s) 634
BstMWI GCNNNNNNNGC 4 cut(s) 32, 41, 161, 293
BstNI CCWGG 1 cut(s) 23
BstPI GGTNACC 1 cut(s) 175
BstSCI CCNGG 2 cut(s) 21, 63
BstUI CGCG 1 cut(s) 217
BstV1I GCAGC 3 cut(s) 38, 539, 606
BstV2I GAAGAC 1 cut(s) 397
BsuRI GGCC 1 cut(s) 44
BtgI CCRYGG 3 cut(s) 30, 215, 320
BtsCI GGATG 2 cut(s) 316, 488
Cac8I GCNNGC 1 cut(s) 129
CfoI GCGC 1 cut(s) 164
Cfr42I CCGCGG 1 cut(s) 218
Csp6I GTAC 3 cut(s) 259, 349, 616
CviAII CATG 3 cut(s) 199, 415, 532
CviQI GTAC 3 cut(s) 259, 349, 616
DinI GGCGCC 1 cut(s) 163
DpnI GATC 1 cut(s) 636
DpnII GATC 1 cut(s) 634
DrdI GACNNNNNNGTC 1 cut(s) 76
DseDI GACNNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 2 cut(s) 334, 404
EarI CTCTTC 2 cut(s) 334, 404
Ecl136II GAGCTC 1 cut(s) 327
Eco24I GRGCYC 1 cut(s) 329
Eco31I GGTCTC 1 cut(s) 62
Eco53kI GAGCTC 1 cut(s) 327
Eco57I CTGAAG 1 cut(s) 221
Eco91I GGTNACC 1 cut(s) 175
EcoICRI GAGCTC 1 cut(s) 327
EcoO65I GGTNACC 1 cut(s) 175
EcoRII CCWGG 1 cut(s) 21
EcoT38I GRGCYC 1 cut(s) 329
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
FaeI CATG 3 cut(s) 202, 418, 535
FaiI YATR 8 cut(s) 185, 200, 280, 416, 444, 533, 551, 605
FatI CATG 3 cut(s) 198, 414, 531
FblI GTMKAC 1 cut(s) 628
Fnu4HI GCNGC 6 cut(s) 27, 165, 190, 218, 528, 595
FokI GGATG 2 cut(s) 303, 495
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 6 cut(s) 27, 165, 190, 218, 528, 595
GlaI GCGC 1 cut(s) 163
GluI GCNGC 6 cut(s) 27, 165, 190, 218, 528, 595
GsaI CCCAGC 1 cut(s) 300
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 1 cut(s) 44
HapII CCGG 2 cut(s) 65, 254
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 3 cut(s) 202, 418, 535
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 5 cut(s) 13, 18, 208, 232, 553
HpaII CCGG 2 cut(s) 65, 254
HphI GGTGA 2 cut(s) 169, 382
Hpy166II GTNNAC 3 cut(s) 12, 514, 629
Hpy188I TCNGA 4 cut(s) 50, 88, 96, 240
Hpy188III TCNNGA 4 cut(s) 205, 229, 265, 424
Hpy8I GTNNAC 3 cut(s) 12, 514, 629
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 1 cut(s) 92
HpyCH4V TGCA 6 cut(s) 287, 316, 380, 499, 506, 607
HpyF10VI GCNNNNNNNGC 4 cut(s) 32, 41, 161, 293
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 3 cut(s) 202, 418, 535
HspAI GCGC 1 cut(s) 162
KasI GGCGCC 1 cut(s) 161
KspI CCGCGG 1 cut(s) 218
Kzo9I GATC 1 cut(s) 634
LguI GCTCTTC 1 cut(s) 334
LmnI GCTCC 2 cut(s) 324, 457
Lsp1109I GCAGC 3 cut(s) 38, 539, 606
LweI GCATC 3 cut(s) 201, 229, 325
MaeIII GTNAC 1 cut(s) 175
MalI GATC 1 cut(s) 636
MbiI CCGCTC 1 cut(s) 167
MboI GATC 1 cut(s) 634
MboII GAAGA 7 cut(s) 203, 227, 321, 391, 397, 485, 574
MhlI GDGCHC 1 cut(s) 329
MlsI TGGCCA 1 cut(s) 44
MluNI TGGCCA 1 cut(s) 44
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 7
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 3 cut(s) 119, 343, 447
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MslI CAYNNNNRTG 5 cut(s) 50, 197, 269, 413, 425
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 2 cut(s) 217, 296
MspI CCGG 2 cut(s) 65, 254
MspR9I CCNGG 2 cut(s) 23, 65
Mva1269I GAATGC 2 cut(s) 155, 380
MvaI CCWGG 1 cut(s) 23
MvnI CGCG 1 cut(s) 217
MwoI GCNNNNNNNGC 4 cut(s) 32, 41, 161, 293
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 1 cut(s) 65
NdeII GATC 1 cut(s) 634
NlaIII CATG 3 cut(s) 202, 418, 535
NlaIV GGNNCC 1 cut(s) 163
NmeAIII GCCGAG 1 cut(s) 336
NmuCI GTSAC 1 cut(s) 175
OliI CACNNNNGTG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 334
PctI GAATGC 2 cut(s) 155, 380
PfeI GAWTC 4 cut(s) 18, 208, 232, 553
PkrI GCNGC 6 cut(s) 28, 166, 191, 219, 529, 596
PleI GAGTC 1 cut(s) 7
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 7
Psp124BI GAGCTC 1 cut(s) 329
Psp6I CCWGG 1 cut(s) 21
PspEI GGTNACC 1 cut(s) 175
PspFI CCCAGC 1 cut(s) 296
PspGI CCWGG 1 cut(s) 21
PspN4I GGNNCC 1 cut(s) 163
PvuII CAGCTG 1 cut(s) 296
RsaI GTAC 3 cut(s) 260, 350, 617
RsaNI GTAC 3 cut(s) 259, 349, 616
RseI CAYNNNNRTG 5 cut(s) 50, 197, 269, 413, 425
SacI GAGCTC 1 cut(s) 329
SacII CCGCGG 1 cut(s) 218
SapI GCTCTTC 1 cut(s) 334
SatI GCNGC 6 cut(s) 27, 165, 190, 218, 528, 595
Sau3AI GATC 1 cut(s) 634
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 2 cut(s) 23, 65
SduI GDGCHC 1 cut(s) 329
SfaNI GCATC 3 cut(s) 201, 229, 325
SfoI GGCGCC 1 cut(s) 163
Sfr303I CCGCGG 1 cut(s) 218
SgrBI CCGCGG 1 cut(s) 218
SmiMI CAYNNNNRTG 5 cut(s) 50, 197, 269, 413, 425
SsiI CCGC 5 cut(s) 153, 165, 189, 215, 217
SspDI GGCGCC 1 cut(s) 161
SstI GAGCTC 1 cut(s) 329
StyD4I CCNGG 2 cut(s) 21, 63
TaaI ACNGT 1 cut(s) 92
TaqI TCGA 3 cut(s) 16, 80, 423
TauI GCSGC 3 cut(s) 167, 192, 220
TfiI GAWTC 4 cut(s) 18, 208, 232, 553
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 3 cut(s) 26, 527, 594
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 3 cut(s) 172, 446, 548
TspGWI ACGGA 1 cut(s) 309
XmiI GTMKAC 1 cut(s) 628
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.