Rw7G033970

Transmembrane protein 205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0003018
Physical Location & Seq
Reverse (-)
50151042 .. 50152413
1372 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G033970.1

Sequence Viewer

Length: 468 bp
ATGCATTGGCTGACTTTCGAAGATTTACTTTTCTGGGGAAACAACATAGATTGCATCTGGTTCATGTTAAGCCTCTGGACAGAGAGATTTGCGGGCAATCTTCTGAAGCATCAGTTCGGTAATCTGCAAAGCAAGATGTTACCGGCATGTTTCACCATGGTGGGGATTTGCTGTGCAATATCAGTATCTTCATTTGGGTATTTGCATCCATGGAACTCTTCCACTGTAGCAGACAAGTACCAACTCGGTCTTTTGCTTTCAATCTCACCAATTTGTAGAGAGGGAACAGAATATTGGGGAAGAAGTGGGATGGTCCAAAAATGTGCAAGTTGCGAAGGTGAACCGAAACTAGCAGCCATGAATAAGAAGTTCGGGATGATTCACGGGTTACTATCTCTTGCCAATATTATGGCCTTTGGCAGCCTTGCCATGCACTCACGGTACTTTGCAGGTAAAATTAATTTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

155

Amino Acids

17.42

Weight (kDa)

8.5

Isoelectric Point (pI)

46.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 33 - 88 3.9e-06 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 440
AciI CCGC 1 cut(s) 92
AcuI CTGAAG 1 cut(s) 125
AfaI GTAC 2 cut(s) 239, 443
AfiI CCNNNNNNNGG 1 cut(s) 162
AgsI TTSAA 1 cut(s) 261
AjuI GAANNNNNNNTTGG 2 cut(s) 277, 309
AleI CACNNNNGTG 1 cut(s) 158
AoxI GGCC 1 cut(s) 411
ApeKI GCWGC 2 cut(s) 353, 420
AseI ATTAAT 1 cut(s) 459
AspS9I GGNCC 1 cut(s) 313
AsuHPI GGTGA 3 cut(s) 145, 258, 350
AsuII TTCGAA 1 cut(s) 18
AvaII GGWCC 1 cut(s) 313
BbvI GCAGC 2 cut(s) 365, 432
BccI CCATC 1 cut(s) 304
BfaI CTAG 1 cut(s) 350
BfmI CTRYAG 1 cut(s) 225
BfuAI ACCTGC 1 cut(s) 440
BisI GCNGC 2 cut(s) 354, 421
BlsI GCNGC 2 cut(s) 355, 422
Bme18I GGWCC 1 cut(s) 313
BmgT120I GGNCC 1 cut(s) 313
BmsI GCATC 3 cut(s) 63, 118, 214
Bpu14I TTCGAA 1 cut(s) 18
BsaJI CCNNGG 2 cut(s) 156, 209
Bsc4I CCNNNNNNNGG 1 cut(s) 162
Bse118I RCCGGY 1 cut(s) 142
BseDI CCNNGG 2 cut(s) 156, 209
BseGI GGATG 3 cut(s) 205, 315, 381
BseLI CCNNNNNNNGG 1 cut(s) 162
BseXI GCAGC 2 cut(s) 365, 432
BshFI GGCC 1 cut(s) 413
BsiSI CCGG 1 cut(s) 143
BslI CCNNNNNNNGG 1 cut(s) 162
BsnI GGCC 1 cut(s) 413
Bsp119I TTCGAA 1 cut(s) 18
Bsp19I CCATGG 2 cut(s) 156, 209
BspACI CCGC 1 cut(s) 92
BspANI GGCC 1 cut(s) 413
BspMI ACCTGC 1 cut(s) 440
BspT104I TTCGAA 1 cut(s) 18
BsrFI RCCGGY 1 cut(s) 142
BssAI RCCGGY 1 cut(s) 142
BssECI CCNNGG 2 cut(s) 156, 209
BssT1I CCWWGG 2 cut(s) 156, 209
Bst4CI ACNGT 2 cut(s) 226, 441
Bst6I CTCTTC 1 cut(s) 223
BstBI TTCGAA 1 cut(s) 18
BstC8I GCNNGC 1 cut(s) 94
BstDSI CCRYGG 2 cut(s) 156, 209
BstF5I GGATG 3 cut(s) 205, 315, 381
BstNSI RCATGY 1 cut(s) 150
BstSFI CTRYAG 1 cut(s) 225
BstV1I GCAGC 2 cut(s) 365, 432
BstXI CCANNNNNNTGG 1 cut(s) 409
BsuRI GGCC 1 cut(s) 413
BtgI CCRYGG 2 cut(s) 156, 209
BtsCI GGATG 3 cut(s) 205, 315, 381
BtsIMutI CAGTG 1 cut(s) 222
BveI ACCTGC 1 cut(s) 440
Cac8I GCNNGC 1 cut(s) 94
Cfr10I RCCGGY 1 cut(s) 142
Cfr13I GGNCC 1 cut(s) 313
Csp6I GTAC 2 cut(s) 238, 442
CviAII CATG 6 cut(s) 64, 147, 157, 210, 358, 430
CviJI RGCY 5 cut(s) 10, 72, 356, 413, 423
CviKI_1 RGCY 5 cut(s) 10, 72, 356, 413, 423
CviQI GTAC 2 cut(s) 238, 442
Eam1104I CTCTTC 1 cut(s) 223
EarI CTCTTC 1 cut(s) 223
Eco130I CCWWGG 2 cut(s) 156, 209
Eco47I GGWCC 1 cut(s) 313
Eco57I CTGAAG 1 cut(s) 125
EcoT14I CCWWGG 2 cut(s) 156, 209
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 156, 209
FaeI CATG 6 cut(s) 67, 150, 160, 213, 361, 433
FaiI YATR 8 cut(s) 47, 65, 148, 158, 211, 359, 410, 431
FalI AAGNNNNNCTT 2 cut(s) 12, 44
FatI CATG 6 cut(s) 63, 146, 156, 209, 357, 429
FauI CCCGC 1 cut(s) 85
Fnu4HI GCNGC 2 cut(s) 354, 421
FokI GGATG 3 cut(s) 192, 322, 388
Fsp4HI GCNGC 2 cut(s) 354, 421
FspBI CTAG 1 cut(s) 350
GluI GCNGC 2 cut(s) 354, 421
HaeIII GGCC 1 cut(s) 413
HapII CCGG 1 cut(s) 143
Hin1II CATG 6 cut(s) 67, 150, 160, 213, 361, 433
HinfI GANTC 1 cut(s) 379
HpaII CCGG 1 cut(s) 143
HphI GGTGA 3 cut(s) 145, 258, 350
Hpy166II GTNNAC 1 cut(s) 341
Hpy188I TCNGA 1 cut(s) 105
Hpy188III TCNNGA 2 cut(s) 76, 373
Hpy8I GTNNAC 1 cut(s) 341
HpyAV CCTTC 1 cut(s) 329
HpyCH4III ACNGT 2 cut(s) 226, 441
HpyCH4V TGCA 8 cut(s) 4, 54, 127, 176, 205, 326, 433, 449
Hsp92II CATG 6 cut(s) 67, 150, 160, 213, 361, 433
LpnPI CCDG 5 cut(s) 19, 43, 61, 156, 435
Lsp1109I GCAGC 2 cut(s) 365, 432
LweI GCATC 3 cut(s) 63, 118, 214
MaeI CTAG 1 cut(s) 350
MaeIII GTNAC 2 cut(s) 138, 387
MboII GAAGA 5 cut(s) 32, 92, 180, 210, 312
MluCI AATT 3 cut(s) 270, 456, 460
MnlI CCTC 2 cut(s) 83, 274
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 2 cut(s) 68, 459
MslI CAYNNNNRTG 1 cut(s) 158
MspI CCGG 1 cut(s) 143
NcoI CCATGG 2 cut(s) 156, 209
NlaIII CATG 6 cut(s) 67, 150, 160, 213, 361, 433
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 150
NspV TTCGAA 1 cut(s) 18
OliI CACNNNNGTG 1 cut(s) 158
PfeI GAWTC 1 cut(s) 379
PkrI GCNGC 2 cut(s) 355, 422
PshBI ATTAAT 1 cut(s) 459
PspPI GGNCC 1 cut(s) 313
RsaI GTAC 2 cut(s) 239, 443
RsaNI GTAC 2 cut(s) 238, 442
RseI CAYNNNNRTG 1 cut(s) 158
SaqAI TTAA 2 cut(s) 68, 459
SatI GCNGC 2 cut(s) 354, 421
Sau96I GGNCC 1 cut(s) 313
SetI ASST 2 cut(s) 340, 454
SfaNI GCATC 3 cut(s) 63, 118, 214
SfcI CTRYAG 1 cut(s) 225
SfuI TTCGAA 1 cut(s) 18
SinI GGWCC 1 cut(s) 313
SmiMI CAYNNNNRTG 1 cut(s) 158
Sse9I AATT 3 cut(s) 270, 456, 460
SsiI CCGC 1 cut(s) 92
SspI AATATT 2 cut(s) 293, 406
SspMI CTAG 1 cut(s) 350
StyI CCWWGG 2 cut(s) 156, 209
TaaI ACNGT 2 cut(s) 226, 441
TaqI TCGA 1 cut(s) 18
TaqII GACCGA 1 cut(s) 236
TasI AATT 3 cut(s) 270, 456, 460
TfiI GAWTC 1 cut(s) 379
Tru1I TTAA 2 cut(s) 68, 459
Tru9I TTAA 2 cut(s) 68, 459
TscAI CASTG 1 cut(s) 229
TseI GCWGC 2 cut(s) 353, 420
TspDTI ATGAA 3 cut(s) 52, 180, 374
TspRI CASTG 1 cut(s) 229
VpaK11BI GGWCC 1 cut(s) 313
VspI ATTAAT 1 cut(s) 459
XceI RCATGY 1 cut(s) 150
XspI CTAG 1 cut(s) 350
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.