pycom03g23530

Transmembrane protein 205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0003018
Physical Location & Seq
Forward (+)
23329130 .. 23329360
231 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g23530.1

Sequence Viewer

Length: 231 bp
ATGGCTTGGTTGACTCGATTTCTGGCAGCCGTGGCTTTCTTGGCCATCGGAGTGATATTCTCCCCGGAGACCTTTGGGTCGAAATCGGACGGTCTGAAATCGCCCACCCTCTCCACCTACCTAAAGCTGGCACATCTGCTCTGTTTCTCCACCGTTTTCGGCGCCGCTCTCTGGGTCACCTTCATAGGCGGCATCATCATGTTCAAGTATTCATTTTTTATCTTGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

77

Amino Acids

8.33

Weight (kDa)

9.43

Isoelectric Point (pI)

26.57

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 76
AccB1I GGYRCC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 167
AciI CCGC 2 cut(s) 165, 189
AcoI YGGCCR 1 cut(s) 42
AcyI GRCGYC 1 cut(s) 162
AfiI CCNNNNNNNGG 2 cut(s) 127, 171
AgsI TTSAA 1 cut(s) 205
AluBI AGCT 1 cut(s) 127
AluI AGCT 1 cut(s) 127
Alw26I GTCTC 1 cut(s) 62
AoxI GGCC 1 cut(s) 42
ApeKI GCWGC 1 cut(s) 26
AspLEI GCGC 1 cut(s) 164
AsuC2I CCSGG 1 cut(s) 65
AsuHPI GGTGA 1 cut(s) 169
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 161
BbvI GCAGC 1 cut(s) 38
BccI CCATC 1 cut(s) 53
BceAI ACGGC 1 cut(s) 14
BcnI CCSGG 1 cut(s) 65
BcoDI GTCTC 1 cut(s) 62
BfoI RGCGCY 1 cut(s) 165
BisI GCNGC 3 cut(s) 27, 165, 190
BlsI GCNGC 3 cut(s) 28, 166, 191
Bme1390I CCNGG 1 cut(s) 65
BmiI GGNNCC 1 cut(s) 163
BmrFI CCNGG 1 cut(s) 65
BmsI GCATC 1 cut(s) 201
BpuMI CCSGG 1 cut(s) 65
BsaHI GRCGYC 1 cut(s) 162
BsaI GGTCTC 1 cut(s) 62
BsaJI CCNNGG 2 cut(s) 30, 63
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 171
BseDI CCNNGG 2 cut(s) 30, 63
BseLI CCNNNNNNNGG 2 cut(s) 127, 171
BseXI GCAGC 1 cut(s) 38
BshFI GGCC 1 cut(s) 44
BshNI GGYRCC 1 cut(s) 161
BsiSI CCGG 1 cut(s) 65
BslI CCNNNNNNNGG 2 cut(s) 127, 171
BsmAI GTCTC 1 cut(s) 62
BsnI GGCC 1 cut(s) 44
Bso31I GGTCTC 1 cut(s) 62
BspACI CCGC 2 cut(s) 165, 189
BspANI GGCC 1 cut(s) 44
BspLI GGNNCC 1 cut(s) 163
BspT107I GGYRCC 1 cut(s) 161
BspTNI GGTCTC 1 cut(s) 62
BsrBI CCGCTC 1 cut(s) 167
BssECI CCNNGG 2 cut(s) 30, 63
BssNI GRCGYC 1 cut(s) 162
Bst4CI ACNGT 2 cut(s) 92, 154
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 1 cut(s) 129
BstDSI CCRYGG 1 cut(s) 30
BstEII GGTNACC 1 cut(s) 175
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstMAI GTCTC 1 cut(s) 62
BstMWI GCNNNNNNNGC 2 cut(s) 32, 41
BstPI GGTNACC 1 cut(s) 175
BstSCI CCNGG 1 cut(s) 63
BstV1I GCAGC 1 cut(s) 38
BsuRI GGCC 1 cut(s) 44
BtgI CCRYGG 1 cut(s) 30
Cac8I GCNNGC 1 cut(s) 129
CfoI GCGC 1 cut(s) 164
CviAII CATG 1 cut(s) 199
CviJI RGCY 5 cut(s) 5, 29, 35, 44, 127
CviKI_1 RGCY 5 cut(s) 5, 29, 35, 44, 127
DinI GGCGCC 1 cut(s) 163
DrdI GACNNNNNNGTC 1 cut(s) 76
DseDI GACNNNNNNGTC 1 cut(s) 76
EaeI YGGCCR 1 cut(s) 42
Eco31I GGTCTC 1 cut(s) 62
Eco91I GGTNACC 1 cut(s) 175
EcoO65I GGTNACC 1 cut(s) 175
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
FaeI CATG 1 cut(s) 202
FaiI YATR 2 cut(s) 185, 200
FatI CATG 1 cut(s) 198
Fnu4HI GCNGC 3 cut(s) 27, 165, 190
Fsp4HI GCNGC 3 cut(s) 27, 165, 190
GlaI GCGC 1 cut(s) 163
GluI GCNGC 3 cut(s) 27, 165, 190
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 1 cut(s) 44
HapII CCGG 1 cut(s) 65
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 1 cut(s) 202
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HincII GTYRAC 1 cut(s) 12
HindII GTYRAC 1 cut(s) 12
HinfI GANTC 1 cut(s) 13
HpaII CCGG 1 cut(s) 65
HphI GGTGA 1 cut(s) 169
Hpy166II GTNNAC 1 cut(s) 12
Hpy188I TCNGA 3 cut(s) 50, 88, 96
Hpy188III TCNNGA 1 cut(s) 228
Hpy8I GTNNAC 1 cut(s) 12
HpyAV CCTTC 1 cut(s) 190
HpyCH4III ACNGT 2 cut(s) 92, 154
HpyF10VI GCNNNNNNNGC 2 cut(s) 32, 41
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 1 cut(s) 202
HspAI GCGC 1 cut(s) 162
KasI GGCGCC 1 cut(s) 161
LpnPI CCDG 4 cut(s) 8, 78, 113, 157
Lsp1109I GCAGC 1 cut(s) 38
LweI GCATC 1 cut(s) 201
MaeIII GTNAC 1 cut(s) 175
MbiI CCGCTC 1 cut(s) 167
MlsI TGGCCA 1 cut(s) 44
MluNI TGGCCA 1 cut(s) 44
Mly113I GGCGCC 1 cut(s) 162
MlyI GAGTC 1 cut(s) 7
MnlI CCTC 1 cut(s) 119
Mox20I TGGCCA 1 cut(s) 44
MscI TGGCCA 1 cut(s) 44
MslI CAYNNNNRTG 2 cut(s) 50, 197
Msp20I TGGCCA 1 cut(s) 44
MspI CCGG 1 cut(s) 65
MspR9I CCNGG 1 cut(s) 65
MwoI GCNNNNNNNGC 2 cut(s) 32, 41
NarI GGCGCC 1 cut(s) 162
NciI CCSGG 1 cut(s) 65
NlaIII CATG 1 cut(s) 202
NlaIV GGNNCC 1 cut(s) 163
NmuCI GTSAC 1 cut(s) 175
PkrI GCNGC 3 cut(s) 28, 166, 191
PleI GAGTC 1 cut(s) 7
PluTI GGCGCC 1 cut(s) 165
PpsI GAGTC 1 cut(s) 7
PspEI GGTNACC 1 cut(s) 175
PspN4I GGNNCC 1 cut(s) 163
RseI CAYNNNNRTG 2 cut(s) 50, 197
SatI GCNGC 3 cut(s) 27, 165, 190
SchI GAGTC 1 cut(s) 7
ScrFI CCNGG 1 cut(s) 65
SetI ASST 5 cut(s) 74, 119, 123, 129, 182
SfaNI GCATC 1 cut(s) 201
SfoI GGCGCC 1 cut(s) 163
SmiMI CAYNNNNRTG 2 cut(s) 50, 197
SsiI CCGC 2 cut(s) 165, 189
SspDI GGCGCC 1 cut(s) 161
StyD4I CCNGG 1 cut(s) 63
TaaI ACNGT 2 cut(s) 92, 154
TaqI TCGA 2 cut(s) 16, 80
TauI GCSGC 2 cut(s) 167, 192
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 1 cut(s) 26
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 2 cut(s) 172, 201
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.