FvH4_5g39460

Transmembrane protein 205

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0003018
Physical Location & Seq
Forward (+)
29138987 .. 29140771
1785 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g39460.t1

Sequence Viewer

Length: 642 bp
ATGGGTTGGCTGACAAGATCCCTAGCAGCTGTGGCTTTCTTGGCCATCGGAGTTATTTTCTCGCCGGAGACTTTCGGATCGAAATCGGACGGTCCGAAGTCGCCGGCACTCTCCACCTACCTGAAGCTGGCTCATCTGCTCAGCTTCGCCACCGCGTTCGGCGCCGCTCTCTGGGTCACCTTCATCGGCGGCATCATCATGTTCAAGAATCTCCCGAGGCATCAGTTCGGTAATCTGCAGAGCAAGATGTTTCCGGCTTATTTCACCATGGTGGGGATTTGCTGTGCCATTTCAGTAGCTTCGTTCGGGTATTTGCATCCATGGAGCTCTTCCACTGTGGCAGACAAGTACCAGCTCGGCTTTTTGATCTCTTCCTTGGCCTTCAATCTCACCAATTTGTTCGTCTTCACACCCATGACTATTGAGATGATGAAGCAGAGGCATAAGATAGAGAAGGAACAGAACATTGGGGAAGAAGTTGGATGGTCCAAGAATGTGCAAGTTGCAAAGGTGAACCCGAAACTAGCAGCCATGAATAAGAAGTTCGGGATGATTCACGGGCTATCTTCTCTCGCCAATATTATGGCCTTTGGCAGCCTTGCCATGCACACATGGTACTTGGCAGGTAAACTTGATCTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4149

Protein Analysis

214

Amino Acids

23.4

Weight (kDa)

9.67

Isoelectric Point (pI)

40.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF4149 PF13664 47 - 149 9.3e-31 Domain of unknown function (DUF4149)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 614
AccB1I GGYRCC 1 cut(s) 161
AccBSI CCGCTC 1 cut(s) 167
AccII CGCG 1 cut(s) 155
AciI CCGC 3 cut(s) 153, 165, 189
AclWI GGATC 2 cut(s) 12, 85
AcoI YGGCCR 1 cut(s) 42
AcuI CTGAAG 1 cut(s) 143
AcyI GRCGYC 1 cut(s) 162
AfaI GTAC 2 cut(s) 350, 617
AfiI CCNNNNNNNGG 3 cut(s) 127, 171, 273
AgsI TTSAA 2 cut(s) 205, 385
AjuI GAANNNNNNNTTGG 2 cut(s) 450, 482
AleI CACNNNNGTG 1 cut(s) 269
AluBI AGCT 6 cut(s) 29, 127, 144, 299, 327, 355
AluI AGCT 6 cut(s) 29, 127, 144, 299, 327, 355
Alw21I GWGCWC 1 cut(s) 329
Alw26I GTCTC 1 cut(s) 62
AlwI GGATC 2 cut(s) 12, 85
Ama87I CYCGRG 1 cut(s) 214
AoxI GGCC 3 cut(s) 42, 378, 585
ApeKI GCWGC 3 cut(s) 26, 527, 594
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 2 cut(s) 92, 486
AsuHPI GGTGA 4 cut(s) 169, 256, 382, 523
AvaI CYCGRG 1 cut(s) 214
AvaII GGWCC 2 cut(s) 92, 486
BalI TGGCCA 1 cut(s) 44
BanI GGYRCC 1 cut(s) 161
BanII GRGCYC 1 cut(s) 329
BbsI GAAGAC 1 cut(s) 397
Bbv12I GWGCWC 1 cut(s) 329
BbvI GCAGC 3 cut(s) 38, 539, 606
BccI CCATC 2 cut(s) 53, 477
BcoDI GTCTC 1 cut(s) 62
BfaI CTAG 2 cut(s) 23, 524
BfmI CTRYAG 1 cut(s) 236
BfoI RGCGCY 1 cut(s) 165
BfuAI ACCTGC 1 cut(s) 614
BisI GCNGC 5 cut(s) 27, 165, 190, 528, 595
BlpI GCTNAGC 1 cut(s) 140
BlsI GCNGC 5 cut(s) 28, 166, 191, 529, 596
Bme18I GGWCC 2 cut(s) 92, 486
BmeT110I CYCGRG 1 cut(s) 214
BmgT120I GGNCC 2 cut(s) 92, 486
BmiI GGNNCC 1 cut(s) 163
BmsI GCATC 3 cut(s) 201, 229, 325
BpiI GAAGAC 1 cut(s) 397
Bpu1102I GCTNAGC 1 cut(s) 140
BsaBI GATNNNNATC 1 cut(s) 82
BsaHI GRCGYC 1 cut(s) 162
BsaJI CCNNGG 4 cut(s) 215, 267, 320, 375
Bsc4I CCNNNNNNNGG 3 cut(s) 127, 171, 273
Bse118I RCCGGY 1 cut(s) 103
Bse8I GATNNNNATC 1 cut(s) 82
BseDI CCNNGG 4 cut(s) 215, 267, 320, 375
BseGI GGATG 3 cut(s) 316, 488, 555
BseJI GATNNNNATC 1 cut(s) 82
BseLI CCNNNNNNNGG 3 cut(s) 127, 171, 273
BseMII CTCAG 1 cut(s) 154
BseXI GCAGC 3 cut(s) 38, 539, 606
Bsh1236I CGCG 1 cut(s) 155
BshFI GGCC 3 cut(s) 44, 380, 587
BshNI GGYRCC 1 cut(s) 161
BsiHKAI GWGCWC 1 cut(s) 329
BsiHKCI CYCGRG 1 cut(s) 214
BsiSI CCGG 3 cut(s) 65, 104, 254
BslI CCNNNNNNNGG 3 cut(s) 127, 171, 273
BsmAI GTCTC 1 cut(s) 62
BsnI GGCC 3 cut(s) 44, 380, 587
BsoBI CYCGRG 1 cut(s) 214
Bsp1286I GDGCHC 1 cut(s) 329
Bsp143I GATC 4 cut(s) 17, 77, 366, 634
Bsp1720I GCTNAGC 1 cut(s) 140
Bsp19I CCATGG 2 cut(s) 267, 320
BspACI CCGC 3 cut(s) 153, 165, 189
BspANI GGCC 3 cut(s) 44, 380, 587
BspCNI CTCAG 1 cut(s) 153
BspFNI CGCG 1 cut(s) 155
BspLI GGNNCC 1 cut(s) 163
BspMAI CTGCAG 1 cut(s) 240
BspMI ACCTGC 1 cut(s) 614
BspPI GGATC 2 cut(s) 12, 85
BspQI GCTCTTC 1 cut(s) 334
BspT107I GGYRCC 1 cut(s) 161
BsrBI CCGCTC 1 cut(s) 167
BsrFI RCCGGY 1 cut(s) 103
BssAI RCCGGY 1 cut(s) 103
BssECI CCNNGG 4 cut(s) 215, 267, 320, 375
BssMI GATC 4 cut(s) 17, 77, 366, 634
BssNI GRCGYC 1 cut(s) 162
BssT1I CCWWGG 3 cut(s) 267, 320, 375
Bst4CI ACNGT 2 cut(s) 92, 337
Bst6I CTCTTC 2 cut(s) 334, 376
BstACI GRCGYC 1 cut(s) 162
BstC8I GCNNGC 2 cut(s) 105, 129
BstDEI CTNAG 1 cut(s) 140
BstDSI CCRYGG 2 cut(s) 267, 320
BstEII GGTNACC 1 cut(s) 175
BstF5I GGATG 3 cut(s) 316, 488, 555
BstFNI CGCG 1 cut(s) 155
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstKTI GATC 4 cut(s) 20, 80, 369, 637
BstMAI GTCTC 1 cut(s) 62
BstMBI GATC 4 cut(s) 17, 77, 366, 634
BstMWI GCNNNNNNNGC 3 cut(s) 32, 41, 161
BstPI GGTNACC 1 cut(s) 175
BstSFI CTRYAG 1 cut(s) 236
BstUI CGCG 1 cut(s) 155
BstV1I GCAGC 3 cut(s) 38, 539, 606
BstV2I GAAGAC 1 cut(s) 397
BstX2I RGATCY 1 cut(s) 17
BstXI CCANNNNNNTGG 1 cut(s) 583
BstYI RGATCY 1 cut(s) 17
BsuRI GGCC 3 cut(s) 44, 380, 587
BtgI CCRYGG 2 cut(s) 267, 320
BtsCI GGATG 3 cut(s) 316, 488, 555
BtsIMutI CAGTG 1 cut(s) 333
BveI ACCTGC 1 cut(s) 614
Cac8I GCNNGC 2 cut(s) 105, 129
CfoI GCGC 1 cut(s) 164
Cfr10I RCCGGY 1 cut(s) 103
Cfr13I GGNCC 2 cut(s) 92, 486
CpoI CGGWCCG 1 cut(s) 92
Csp6I GTAC 2 cut(s) 349, 616
CspI CGGWCCG 1 cut(s) 92
CviAII CATG 7 cut(s) 199, 268, 321, 415, 532, 604, 612
CviQI GTAC 2 cut(s) 349, 616
DdeI CTNAG 1 cut(s) 140
DinI GGCGCC 1 cut(s) 163
DpnI GATC 4 cut(s) 19, 79, 368, 636
DpnII GATC 4 cut(s) 17, 77, 366, 634
EaeI YGGCCR 1 cut(s) 42
Eam1104I CTCTTC 2 cut(s) 334, 376
EarI CTCTTC 2 cut(s) 334, 376
Ecl136II GAGCTC 1 cut(s) 327
Eco130I CCWWGG 3 cut(s) 267, 320, 375
Eco24I GRGCYC 1 cut(s) 329
Eco47I GGWCC 2 cut(s) 92, 486
Eco53kI GAGCTC 1 cut(s) 327
Eco57I CTGAAG 1 cut(s) 143
Eco88I CYCGRG 1 cut(s) 214
Eco91I GGTNACC 1 cut(s) 175
EcoICRI GAGCTC 1 cut(s) 327
EcoO65I GGTNACC 1 cut(s) 175
EcoT14I CCWWGG 3 cut(s) 267, 320, 375
EcoT38I GRGCYC 1 cut(s) 329
EgeI GGCGCC 1 cut(s) 163
EheI GGCGCC 1 cut(s) 163
ErhI CCWWGG 3 cut(s) 267, 320, 375
FaeI CATG 7 cut(s) 202, 271, 324, 418, 535, 607, 615
FaiI YATR 9 cut(s) 200, 269, 322, 416, 444, 533, 584, 605, 613
FatI CATG 7 cut(s) 198, 267, 320, 414, 531, 603, 611
Fnu4HI GCNGC 5 cut(s) 27, 165, 190, 528, 595
FokI GGATG 3 cut(s) 303, 495, 562
FriOI GRGCYC 1 cut(s) 329
Fsp4HI GCNGC 5 cut(s) 27, 165, 190, 528, 595
FspBI CTAG 2 cut(s) 23, 524
GlaI GCGC 1 cut(s) 163
GluI GCNGC 5 cut(s) 27, 165, 190, 528, 595
HaeII RGCGCY 1 cut(s) 165
HaeIII GGCC 3 cut(s) 44, 380, 587
HapII CCGG 3 cut(s) 65, 104, 254
HhaI GCGC 1 cut(s) 164
Hin1I GRCGYC 1 cut(s) 162
Hin1II CATG 7 cut(s) 202, 271, 324, 418, 535, 607, 615
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HinfI GANTC 2 cut(s) 208, 553
HpaII CCGG 3 cut(s) 65, 104, 254
HphI GGTGA 4 cut(s) 169, 256, 382, 523
Hpy166II GTNNAC 2 cut(s) 514, 629
Hpy188I TCNGA 4 cut(s) 50, 77, 88, 96
Hpy188III TCNNGA 3 cut(s) 205, 214, 547
Hpy8I GTNNAC 2 cut(s) 514, 629
HpyAV CCTTC 3 cut(s) 190, 391, 448
HpyCH4III ACNGT 2 cut(s) 92, 337
HpyCH4V TGCA 5 cut(s) 238, 316, 499, 506, 607
HpyF10VI GCNNNNNNNGC 3 cut(s) 32, 41, 161
HpyF3I CTNAG 1 cut(s) 140
Hsp92I GRCGYC 1 cut(s) 162
Hsp92II CATG 7 cut(s) 202, 271, 324, 418, 535, 607, 615
HspAI GCGC 1 cut(s) 162
KasI GGCGCC 1 cut(s) 161
KroI GCCGGC 1 cut(s) 103
KroNI GCCGGC 1 cut(s) 105
Kzo9I GATC 4 cut(s) 17, 77, 366, 634
LguI GCTCTTC 1 cut(s) 334
LmnI GCTCC 1 cut(s) 324
LpnPI CCDG 8 cut(s) 78, 113, 117, 134, 157, 267, 365, 609
Lsp1109I GCAGC 3 cut(s) 38, 539, 606
LweI GCATC 3 cut(s) 201, 229, 325
MaeI CTAG 2 cut(s) 23, 524
MaeIII GTNAC 1 cut(s) 175
MalI GATC 4 cut(s) 19, 79, 368, 636
MbiI CCGCTC 1 cut(s) 167
MboI GATC 4 cut(s) 17, 77, 366, 634
MboII GAAGA 5 cut(s) 321, 363, 397, 485, 558
MflI RGATCY 1 cut(s) 17
MhlI GDGCHC 1 cut(s) 329
MlsI TGGCCA 1 cut(s) 44
MluCI AATT 1 cut(s) 394
MluNI TGGCCA 1 cut(s) 44
Mly113I GGCGCC 1 cut(s) 162
MmeI TCCRAC 1 cut(s) 460
MnlI CCTC 2 cut(s) 210, 432
Mox20I TGGCCA 1 cut(s) 44
MroNI GCCGGC 1 cut(s) 103
MscI TGGCCA 1 cut(s) 44
MslI CAYNNNNRTG 3 cut(s) 197, 269, 413
Msp20I TGGCCA 1 cut(s) 44
MspA1I CMGCKG 1 cut(s) 29
MspI CCGG 3 cut(s) 65, 104, 254
MvnI CGCG 1 cut(s) 155
MwoI GCNNNNNNNGC 3 cut(s) 32, 41, 161
NaeI GCCGGC 1 cut(s) 105
NarI GGCGCC 1 cut(s) 162
NcoI CCATGG 2 cut(s) 267, 320
NdeII GATC 4 cut(s) 17, 77, 366, 634
NgoMIV GCCGGC 1 cut(s) 103
NlaIII CATG 7 cut(s) 202, 271, 324, 418, 535, 607, 615
NlaIV GGNNCC 1 cut(s) 163
NmeAIII GCCGAG 1 cut(s) 336
NmuCI GTSAC 1 cut(s) 175
OliI CACNNNNGTG 1 cut(s) 269
PciSI GCTCTTC 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 92
PdiI GCCGGC 1 cut(s) 105
PfeI GAWTC 2 cut(s) 208, 553
PkrI GCNGC 5 cut(s) 28, 166, 191, 529, 596
PluTI GGCGCC 1 cut(s) 165
Psp124BI GAGCTC 1 cut(s) 329
PspEI GGTNACC 1 cut(s) 175
PspN4I GGNNCC 1 cut(s) 163
PspPI GGNCC 2 cut(s) 92, 486
PstI CTGCAG 1 cut(s) 240
PsuI RGATCY 1 cut(s) 17
PvuII CAGCTG 1 cut(s) 29
RsaI GTAC 2 cut(s) 350, 617
RsaNI GTAC 2 cut(s) 349, 616
RseI CAYNNNNRTG 3 cut(s) 197, 269, 413
Rsr2I CGGWCCG 1 cut(s) 92
RsrII CGGWCCG 1 cut(s) 92
SacI GAGCTC 1 cut(s) 329
SapI GCTCTTC 1 cut(s) 334
SatI GCNGC 5 cut(s) 27, 165, 190, 528, 595
Sau3AI GATC 4 cut(s) 17, 77, 366, 634
Sau96I GGNCC 2 cut(s) 92, 486
SduI GDGCHC 1 cut(s) 329
SfaNI GCATC 3 cut(s) 201, 229, 325
SfcI CTRYAG 1 cut(s) 236
SfoI GGCGCC 1 cut(s) 163
SinI GGWCC 2 cut(s) 92, 486
SmiMI CAYNNNNRTG 3 cut(s) 197, 269, 413
Sse9I AATT 1 cut(s) 394
SsiI CCGC 3 cut(s) 153, 165, 189
SspDI GGCGCC 1 cut(s) 161
SspI AATATT 1 cut(s) 580
SspMI CTAG 2 cut(s) 23, 524
SstI GAGCTC 1 cut(s) 329
StyI CCWWGG 3 cut(s) 267, 320, 375
TaaI ACNGT 2 cut(s) 92, 337
TaqI TCGA 1 cut(s) 80
TasI AATT 1 cut(s) 394
TauI GCSGC 2 cut(s) 167, 192
TfiI GAWTC 2 cut(s) 208, 553
TscAI CASTG 1 cut(s) 340
TseFI GTSAC 1 cut(s) 175
TseI GCWGC 3 cut(s) 26, 527, 594
Tsp45I GTSAC 1 cut(s) 175
TspDTI ATGAA 3 cut(s) 172, 446, 548
TspRI CASTG 1 cut(s) 340
VpaK11BI GGWCC 2 cut(s) 92, 486
XspI CTAG 2 cut(s) 23, 524
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.