AT3G62940

OTU-like cysteine protease

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
3
OG0002523
Physical Location & Seq
Reverse (-)
23262795 .. 23264731
1937 bp
Loading structure...
UTR
Exon/CDS
Intron
AT3G62940.5

Sequence Viewer

Length: 951 bp
ATGGATGCTTCTCAGGAGCAACAACATGAAACAGTTGAGGAGATGCTTGCTCGCCACAGGCAAGAGATTAAACAACTTCAAAACAAAGAGACAGAGATGAAGAAGGCAGCTGCCAAAGGTAGCAAAGCTGAACAAAAAGCTAAGAAGAAGCAAGTCGAAGATGACATTTCCAAACTCTCCACTAAGCTCAAGGACAAACAGTTGAAAGAACTCGCCTCTCAAGGTTTCAGCAGCAGCAGCAGCAACAACATTGCCAAGGACGAAACGACTGAGAAAAAAGGAGACATCGACACTCTAGTTAGAGCTATAGCTGGAGTCTCAGTCACTGCTCAGCAAGAGCACTCAAAGCCTAGCAAGAGCGTGAAGAGGCGCGAGAAACGGGCTAAGGAAGAAGCAGACAGAGAGCAGAGAATTAAAGAGGAGCAAAGCCACGTGAAAAGTGATCGTATGGTTGAGAACGCCAAGCTTGAGAAGAAGCTTAAACCATTGGGATTAACAGTCAGTGAGATTAAACCAGACGGACATTGCCTTTACCGTGCTGTTGAGAATCAGCTTGCCAACCGCTCGGGCGGTGCATCTCCTTACACATACCAGAATCTAAGAGAAATGGCTGCTTCGTACATGAGAGAGCACAAAACCGACTTTCTCCCGTTTTTCTTGTCAGAGACTGAGGGTGACTCCAACAGTGGATCTGCAGAAGAGCGGTTTGAGAAATACTGCAGAGAAGTCGAGTCAACCGCTGCTTGGGGCAGTCAATTGGAGCTTGGAGCATTAACACATTGCTTGAGGAAACACATAAAGGTGTATTCGGGATCGTTTCCGGATGTTGAGATGGGCAAAGAGTATAGATCCGGCGATGACTCGAGCCTCATGTTATCGTATCACAGGCATGCTTTTGGGCTCGGGGAGCATTATAACTCAGTGGTGCTCGTCAACAACATCACCGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
OTU

Protein Analysis

316

Amino Acids

35.67

Weight (kDa)

8.21

Isoelectric Point (pI)

48.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
OTU PF02338 171 - 305 7.5e-38 OTU-like cysteine protease
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 915
AccBSI CCGCTC 2 cut(s) 564, 703
AccII CGCG 1 cut(s) 372
AccIII TCCGGA 1 cut(s) 820
AciI CCGC 4 cut(s) 562, 570, 703, 738
AclWI GGATC 3 cut(s) 697, 820, 843
AcvI CACGTG 1 cut(s) 433
AfaI GTAC 1 cut(s) 620
AfiI CCNNNNNNNGG 1 cut(s) 744
AgsI TTSAA 2 cut(s) 80, 205
Alw21I GWGCWC 3 cut(s) 342, 633, 930
Alw26I GTCTC 4 cut(s) 83, 276, 322, 659
AlwI GGATC 3 cut(s) 697, 820, 843
AlwNI CAGNNNCTG 2 cut(s) 326, 668
Ama87I CYCGRG 3 cut(s) 565, 862, 902
Aor13HI TCCGGA 1 cut(s) 820
ApeKI GCWGC 8 cut(s) 107, 110, 231, 234, 237, 240, 611, 740
AspLEI GCGC 1 cut(s) 372
AsuHPI GGTGA 2 cut(s) 686, 934
AvaI CYCGRG 3 cut(s) 565, 862, 902
BanII GRGCYC 1 cut(s) 903
BbrPI CACGTG 1 cut(s) 433
Bbv12I GWGCWC 3 cut(s) 342, 633, 930
BbvI GCAGC 8 cut(s) 97, 119, 243, 246, 249, 252, 598, 727
BccI CCATC 1 cut(s) 826
BcgI CGANNNNNNTGC 2 cut(s) 709, 743
BcoDI GTCTC 4 cut(s) 83, 276, 322, 659
BfaI CTAG 2 cut(s) 296, 351
BfmI CTRYAG 3 cut(s) 306, 693, 718
BisI GCNGC 8 cut(s) 108, 111, 232, 235, 238, 241, 612, 741
BlpI GCTNAGC 1 cut(s) 330
BlsI GCNGC 8 cut(s) 109, 112, 233, 236, 239, 242, 613, 742
BmeT110I CYCGRG 3 cut(s) 565, 862, 902
BmsI GCATC 2 cut(s) 33, 584
BplI GAGNNNNNCTC 2 cut(s) 662, 694
BpmI CTGGAG 1 cut(s) 333
Bpu10I CCTNAGC 1 cut(s) 384
Bpu1102I GCTNAGC 1 cut(s) 330
BpuEI CTTGAG 4 cut(s) 173, 204, 488, 805
BsaAI YACGTR 1 cut(s) 433
BsaJI CCNNGG 1 cut(s) 255
BsaWI WCCGGW 2 cut(s) 820, 944
BsaXI ACNNNNNCTCC 2 cut(s) 306, 336
Bsc4I CCNNNNNNNGG 1 cut(s) 744
Bse3DI GCAATG 3 cut(s) 249, 523, 778
BseAI TCCGGA 1 cut(s) 820
BseDI CCNNGG 1 cut(s) 255
BseGI GGATG 2 cut(s) 10, 829
BseLI CCNNNNNNNGG 1 cut(s) 744
BseMI GCAATG 3 cut(s) 249, 523, 778
BseMII CTCAG 6 cut(s) 26, 261, 333, 344, 660, 933
BseRI GAGGAG 2 cut(s) 53, 434
BseXI GCAGC 8 cut(s) 97, 119, 243, 246, 249, 252, 598, 727
Bsh1236I CGCG 1 cut(s) 372
BsiHKAI GWGCWC 3 cut(s) 342, 633, 930
BsiHKCI CYCGRG 3 cut(s) 565, 862, 902
BsiSI CCGG 3 cut(s) 821, 852, 945
BslI CCNNNNNNNGG 1 cut(s) 744
BsmAI GTCTC 4 cut(s) 83, 276, 322, 659
BsoBI CYCGRG 3 cut(s) 565, 862, 902
Bsp1286I GDGCHC 4 cut(s) 342, 633, 903, 930
Bsp13I TCCGGA 1 cut(s) 820
Bsp143I GATC 4 cut(s) 442, 689, 812, 848
Bsp1720I GCTNAGC 1 cut(s) 330
BspACI CCGC 4 cut(s) 562, 570, 703, 738
BspCNI CTCAG 6 cut(s) 25, 262, 332, 343, 661, 932
BspEI TCCGGA 1 cut(s) 820
BspFNI CGCG 1 cut(s) 372
BspMAI CTGCAG 2 cut(s) 697, 722
BspPI GGATC 3 cut(s) 697, 820, 843
BspQI GCTCTTC 1 cut(s) 693
BsrBI CCGCTC 2 cut(s) 564, 703
BsrDI GCAATG 3 cut(s) 249, 523, 778
BssECI CCNNGG 1 cut(s) 255
BssMI GATC 4 cut(s) 442, 689, 812, 848
BssT1I CCWWGG 1 cut(s) 255
Bst4CI ACNGT 5 cut(s) 34, 201, 499, 536, 686
Bst6I CTCTTC 2 cut(s) 359, 693
BstBAI YACGTR 1 cut(s) 433
BstC8I GCNNGC 4 cut(s) 48, 52, 555, 891
BstF5I GGATG 2 cut(s) 10, 829
BstFNI CGCG 1 cut(s) 372
BstHHI GCGC 1 cut(s) 372
BstKTI GATC 4 cut(s) 445, 692, 815, 851
BstMAI GTCTC 4 cut(s) 83, 276, 322, 659
BstMBI GATC 4 cut(s) 442, 689, 812, 848
BstMWI GCNNNNNNNGC 4 cut(s) 237, 240, 346, 907
BstNSI RCATGY 1 cut(s) 893
BstSFI CTRYAG 3 cut(s) 306, 693, 718
BstUI CGCG 1 cut(s) 372
BstV1I GCAGC 8 cut(s) 97, 119, 243, 246, 249, 252, 598, 727
BstX2I RGATCY 2 cut(s) 689, 848
BstYI RGATCY 2 cut(s) 689, 848
BtgZI GCGATG 1 cut(s) 870
BtsCI GGATG 2 cut(s) 10, 829
BtsI GCAGTG 1 cut(s) 324
BtsIMutI CAGTG 4 cut(s) 324, 508, 691, 927
Cac8I GCNNGC 4 cut(s) 48, 52, 555, 891
CaiI CAGNNNCTG 2 cut(s) 326, 668
CfoI GCGC 1 cut(s) 372
Csp6I GTAC 1 cut(s) 619
CviAII CATG 4 cut(s) 26, 622, 871, 890
CviQI GTAC 1 cut(s) 619
DpnI GATC 4 cut(s) 444, 691, 814, 850
DpnII GATC 4 cut(s) 442, 689, 812, 848
Eam1104I CTCTTC 2 cut(s) 359, 693
EarI CTCTTC 2 cut(s) 359, 693
Eco130I CCWWGG 1 cut(s) 255
Eco24I GRGCYC 1 cut(s) 903
Eco72I CACGTG 1 cut(s) 433
Eco88I CYCGRG 3 cut(s) 565, 862, 902
EcoT14I CCWWGG 1 cut(s) 255
EcoT38I GRGCYC 1 cut(s) 903
ErhI CCWWGG 1 cut(s) 255
FaeI CATG 4 cut(s) 29, 625, 874, 893
FatI CATG 4 cut(s) 25, 621, 870, 889
Fnu4HI GCNGC 8 cut(s) 108, 111, 232, 235, 238, 241, 612, 741
FokI GGATG 2 cut(s) 17, 836
FriOI GRGCYC 1 cut(s) 903
Fsp4HI GCNGC 8 cut(s) 108, 111, 232, 235, 238, 241, 612, 741
FspBI CTAG 2 cut(s) 296, 351
GlaI GCGC 1 cut(s) 371
GluI GCNGC 8 cut(s) 108, 111, 232, 235, 238, 241, 612, 741
GsuI CTGGAG 1 cut(s) 333
HapII CCGG 3 cut(s) 821, 852, 945
HhaI GCGC 1 cut(s) 372
Hin1II CATG 4 cut(s) 29, 625, 874, 893
Hin6I GCGC 1 cut(s) 370
HinP1I GCGC 1 cut(s) 370
HincII GTYRAC 2 cut(s) 735, 934
HindII GTYRAC 2 cut(s) 735, 934
HindIII AAGCTT 2 cut(s) 464, 476
HinfI GANTC 6 cut(s) 315, 547, 595, 677, 731, 860
HpaII CCGG 3 cut(s) 821, 852, 945
HphI GGTGA 2 cut(s) 686, 934
Hpy166II GTNNAC 2 cut(s) 735, 934
Hpy188I TCNGA 1 cut(s) 664
Hpy188III TCNNGA 3 cut(s) 14, 810, 821
Hpy8I GTNNAC 2 cut(s) 735, 934
HpyAV CCTTC 1 cut(s) 97
HpyCH4III ACNGT 5 cut(s) 34, 201, 499, 536, 686
HpyCH4IV ACGT 1 cut(s) 432
HpyCH4V TGCA 3 cut(s) 575, 695, 720
HpyF10VI GCNNNNNNNGC 4 cut(s) 237, 240, 346, 907
HpySE526I ACGT 1 cut(s) 432
Hsp92II CATG 4 cut(s) 29, 625, 874, 893
HspAI GCGC 1 cut(s) 370
Kpn2I TCCGGA 1 cut(s) 820
Kzo9I GATC 4 cut(s) 442, 689, 812, 848
LguI GCTCTTC 1 cut(s) 693
LmnI GCTCC 5 cut(s) 16, 421, 760, 767, 907
LpnPI CCDG 7 cut(s) 43, 297, 528, 605, 834, 865, 871
Lsp1109I GCAGC 8 cut(s) 97, 119, 243, 246, 249, 252, 598, 727
LweI GCATC 2 cut(s) 33, 584
MaeI CTAG 2 cut(s) 296, 351
MaeII ACGT 1 cut(s) 432
MaeIII GTNAC 2 cut(s) 322, 674
MalI GATC 4 cut(s) 444, 691, 814, 850
MbiI CCGCTC 2 cut(s) 564, 703
MboI GATC 4 cut(s) 442, 689, 812, 848
MboII GAAGA 7 cut(s) 112, 157, 170, 376, 401, 484, 710
MfeI CAATTG 1 cut(s) 755
MflI RGATCY 2 cut(s) 689, 848
MhlI GDGCHC 4 cut(s) 342, 633, 903, 930
MluCI AATT 2 cut(s) 411, 755
MlyI GAGTC 4 cut(s) 324, 671, 740, 854
MmeI TCCRAC 1 cut(s) 705
MnlI CCTC 7 cut(s) 31, 226, 360, 412, 664, 780, 878
MroI TCCGGA 1 cut(s) 820
MseI TTAA 6 cut(s) 69, 414, 480, 494, 510, 773
MslI CAYNNNNRTG 2 cut(s) 800, 888
MspA1I CMGCKG 2 cut(s) 110, 740
MspI CCGG 3 cut(s) 821, 852, 945
MunI CAATTG 1 cut(s) 755
MvnI CGCG 1 cut(s) 372
MwoI GCNNNNNNNGC 4 cut(s) 237, 240, 346, 907
NdeII GATC 4 cut(s) 442, 689, 812, 848
NlaIII CATG 4 cut(s) 29, 625, 874, 893
NmuCI GTSAC 2 cut(s) 322, 674
NspI RCATGY 1 cut(s) 893
PaeI GCATGC 1 cut(s) 893
PaeR7I CTCGAG 1 cut(s) 862
PciSI GCTCTTC 1 cut(s) 693
PfeI GAWTC 2 cut(s) 547, 595
PkrI GCNGC 8 cut(s) 109, 112, 233, 236, 239, 242, 613, 742
PleI GAGTC 4 cut(s) 323, 671, 739, 854
PmaCI CACGTG 1 cut(s) 433
PmlI CACGTG 1 cut(s) 433
PpsI GAGTC 4 cut(s) 323, 671, 739, 854
Ppu21I YACGTR 1 cut(s) 433
PsiI TTATAA 1 cut(s) 915
PspCI CACGTG 1 cut(s) 433
PspXI VCTCGAGB 1 cut(s) 862
PstI CTGCAG 2 cut(s) 697, 722
PstNI CAGNNNCTG 2 cut(s) 326, 668
PsuI RGATCY 2 cut(s) 689, 848
PvuII CAGCTG 1 cut(s) 110
RsaI GTAC 1 cut(s) 620
RsaNI GTAC 1 cut(s) 619
RseI CAYNNNNRTG 2 cut(s) 800, 888
SapI GCTCTTC 1 cut(s) 693
SaqAI TTAA 6 cut(s) 69, 414, 480, 494, 510, 773
SatI GCNGC 8 cut(s) 108, 111, 232, 235, 238, 241, 612, 741
Sau3AI GATC 4 cut(s) 442, 689, 812, 848
SchI GAGTC 4 cut(s) 324, 671, 740, 854
SduI GDGCHC 4 cut(s) 342, 633, 903, 930
SfaNI GCATC 2 cut(s) 33, 584
SfcI CTRYAG 3 cut(s) 306, 693, 718
Sfr274I CTCGAG 1 cut(s) 862
SlaI CTCGAG 1 cut(s) 862
SmiMI CAYNNNNRTG 2 cut(s) 800, 888
SmlI CTYRAG 5 cut(s) 188, 219, 467, 784, 862
SmoI CTYRAG 5 cut(s) 188, 219, 467, 784, 862
SphI GCATGC 1 cut(s) 893
Sse9I AATT 2 cut(s) 411, 755
SsiI CCGC 4 cut(s) 562, 570, 703, 738
SspMI CTAG 2 cut(s) 296, 351
StyI CCWWGG 1 cut(s) 255
TaaI ACNGT 5 cut(s) 34, 201, 499, 536, 686
TaiI ACGT 1 cut(s) 435
TaqI TCGA 4 cut(s) 156, 288, 729, 863
TasI AATT 2 cut(s) 411, 755
TfiI GAWTC 2 cut(s) 547, 595
Tru1I TTAA 6 cut(s) 69, 414, 480, 494, 510, 773
Tru9I TTAA 6 cut(s) 69, 414, 480, 494, 510, 773
TscAI CASTG 4 cut(s) 331, 508, 691, 927
TseFI GTSAC 2 cut(s) 322, 674
TseI GCWGC 8 cut(s) 107, 110, 231, 234, 237, 240, 611, 740
Tsp45I GTSAC 2 cut(s) 322, 674
TspDTI ATGAA 2 cut(s) 42, 113
TspGWI ACGGA 1 cut(s) 534
TspRI CASTG 4 cut(s) 331, 508, 691, 927
XceI RCATGY 1 cut(s) 893
XhoI CTCGAG 1 cut(s) 862
XspI CTAG 2 cut(s) 296, 351
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.